AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0588880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0588880 MtrunA17_Chr4g0005151 92.982 114 8 0 1 114 1 114 6.61e-75 217
Msa0588880 MtrunA17_Chr7g0243451 80.392 102 20 0 1 102 1 102 6.38e-58 174
Msa0588880 MtrunA17_Chr4g0005161 56.863 102 44 0 1 102 1 102 2.16e-39 127
Msa0588880 MtrunA17_Chr7g0238011 44.660 103 57 0 1 103 1 103 1.22e-29 103
Msa0588880 MtrunA17_Chr4g0071771 46.078 102 55 0 1 102 1 102 2.73e-26 94.4
Msa0588880 MtrunA17_Chr4g0071761 41.748 103 60 0 1 103 1 103 1.02e-23 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0588880 AT3G51660.1 59.223 103 42 0 1 103 1 103 1.60e-41 133
Msa0588880 AT5G01650.2 55.882 102 45 0 1 102 1 102 4.26e-40 130
Msa0588880 AT5G01650.3 55.882 102 45 0 1 102 1 102 4.26e-40 130
Msa0588880 AT5G01650.4 55.882 102 45 0 1 102 1 102 7.40e-40 129
Msa0588880 AT5G01650.1 55.882 102 45 0 1 102 1 102 1.12e-39 128
Msa0588880 AT5G57170.2 46.078 102 55 0 1 102 1 102 2.61e-26 94.7
Msa0588880 AT5G57170.1 46.078 102 55 0 1 102 1 102 5.63e-26 94.0

Find 23 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACCAACGTTAATTTAGA+TGG 0.290910 4_2:+11351729 Msa0588880:CDS
CAGTACCATTGGCACAATAT+TGG 0.301904 4_2:+11353215 Msa0588880:CDS
ACTGCTGTTTCCACAATCAT+AGG 0.405897 4_2:+11351786 Msa0588880:CDS
TCAAAAGCATCACTACAGTT+TGG 0.409372 4_2:-11353316 None:intergenic
TCTCAAAGTCTTTGACATCA+TGG 0.431478 4_2:+11353272 Msa0588880:CDS
GAAGAGTGACATACCTTGTC+GGG 0.442658 4_2:-11351813 None:intergenic
GTTCTAGCTTGATCAAAGAT+AGG 0.446427 4_2:-11351765 None:intergenic
GTTTGTGATGGTTCTATTGA+AGG 0.461686 4_2:+11353092 Msa0588880:intron
TGTTCTTGGTATAGAGAGGT+TGG 0.461721 4_2:-11353241 None:intergenic
AACAAAGAACCAGCTGCATA+TGG 0.465591 4_2:+11353141 Msa0588880:CDS
TTTGTGATGGTTCTATTGAA+GGG 0.504158 4_2:+11353093 Msa0588880:CDS
TAACGTTGGTAGAGATGTAA+AGG 0.513197 4_2:-11351719 None:intergenic
ATATGGTGAACTAGTTTCAA+TGG 0.521191 4_2:+11353158 Msa0588880:CDS
AAGAATCTAATCAGTACCAT+TGG 0.529298 4_2:+11353204 Msa0588880:CDS
GGATTCCAATATTGTGCCAA+TGG 0.539625 4_2:-11353220 None:intergenic
ATCTTGTTCTTGGTATAGAG+AGG 0.571975 4_2:-11353245 None:intergenic
ACTAGTTCACCATATGCAGC+TGG 0.580662 4_2:-11353150 None:intergenic
TTGTGATGGTTCTATTGAAG+GGG 0.584227 4_2:+11353094 Msa0588880:CDS
TATGGTGAACTAGTTTCAAT+GGG 0.596371 4_2:+11353159 Msa0588880:CDS
AGAAGAGTGACATACCTTGT+CGG 0.618285 4_2:-11351814 None:intergenic
GGTGAACTAGTTTCAATGGG+TGG 0.635436 4_2:+11353162 Msa0588880:CDS
TTGGTAGAGATGTAAAGGCA+AGG 0.650566 4_2:-11351714 None:intergenic
AATCATAGGAAAACCCGACA+AGG 0.692062 4_2:+11351800 Msa0588880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAAACTAATTTCAAT+GGG + chr4_2:11352318-11352337 Msa0588880:intron 10.0%
!! TTAAATAAACTAATTTCAAT+GGG + chr4_2:11352318-11352337 Msa0588880:intron 10.0%
!!! CACAAAATTAATATGAATTT+TGG + chr4_2:11351909-11351928 Msa0588880:intron 15.0%
!!! ATTTATTTCTCAAAATCAAA+TGG + chr4_2:11352219-11352238 Msa0588880:intron 15.0%
!! GTTAAATAAACTAATTTCAA+TGG + chr4_2:11352317-11352336 Msa0588880:intron 15.0%
!! AAATAAAAAAGAATTTCACT+TGG - chr4_2:11352653-11352672 None:intergenic 15.0%
!!! CACAAAATTAATATGAATTT+TGG + chr4_2:11351909-11351928 Msa0588880:intron 15.0%
!!! ATTTATTTCTCAAAATCAAA+TGG + chr4_2:11352219-11352238 Msa0588880:intron 15.0%
!! GTTAAATAAACTAATTTCAA+TGG + chr4_2:11352317-11352336 Msa0588880:intron 15.0%
!! AAATAAAAAAGAATTTCACT+TGG - chr4_2:11352653-11352672 None:intergenic 15.0%
!!! ATTTTGGATTTTGTTGATTA+TGG + chr4_2:11351925-11351944 Msa0588880:intron 20.0%
!!! TTGTTATTTTCAAACGATAT+TGG - chr4_2:11353126-11353145 None:intergenic 20.0%
!!! ATTTTGGATTTTGTTGATTA+TGG + chr4_2:11351925-11351944 Msa0588880:intron 20.0%
!!! TTGTTATTTTCAAACGATAT+TGG - chr4_2:11353126-11353145 None:intergenic 20.0%
!! TTTTCCATCTAAATTAACGT+TGG - chr4_2:11351736-11351755 None:intergenic 25.0%
!! TGTTTTACAAATAAAGCTTG+TGG + chr4_2:11351846-11351865 Msa0588880:intron 25.0%
!! ACTGATTCATAATGTTTGAT+TGG + chr4_2:11351994-11352013 Msa0588880:intron 25.0%
! AAGTAAGTAAATAGCCAAAT+TGG + chr4_2:11352764-11352783 Msa0588880:intron 25.0%
! CCTCTAATGATAAAAATTGA+TGG - chr4_2:11352883-11352902 None:intergenic 25.0%
!! CCATCAATTTTTATCATTAG+AGG + chr4_2:11352880-11352899 Msa0588880:intron 25.0%
! ACATACATAGTGATATTTCA+AGG + chr4_2:11352913-11352932 Msa0588880:intron 25.0%
!!! ATACCATGCAATTTAGTTTT+GGG - chr4_2:11352964-11352983 None:intergenic 25.0%
!!! TATACCATGCAATTTAGTTT+TGG - chr4_2:11352965-11352984 None:intergenic 25.0%
! GCATGGTATAAAAATTTATG+AGG + chr4_2:11352975-11352994 Msa0588880:intron 25.0%
! TTGAGAAAAAATCTTGTTCT+TGG - chr4_2:11353258-11353277 None:intergenic 25.0%
!! TTTTCCATCTAAATTAACGT+TGG - chr4_2:11351736-11351755 None:intergenic 25.0%
!! TGTTTTACAAATAAAGCTTG+TGG + chr4_2:11351846-11351865 Msa0588880:intron 25.0%
!! ACTGATTCATAATGTTTGAT+TGG + chr4_2:11351994-11352013 Msa0588880:intron 25.0%
! AAGTAAGTAAATAGCCAAAT+TGG + chr4_2:11352764-11352783 Msa0588880:intron 25.0%
! CCTCTAATGATAAAAATTGA+TGG - chr4_2:11352883-11352902 None:intergenic 25.0%
!! CCATCAATTTTTATCATTAG+AGG + chr4_2:11352880-11352899 Msa0588880:intron 25.0%
! ACATACATAGTGATATTTCA+AGG + chr4_2:11352913-11352932 Msa0588880:intron 25.0%
!!! ATACCATGCAATTTAGTTTT+GGG - chr4_2:11352964-11352983 None:intergenic 25.0%
!!! TATACCATGCAATTTAGTTT+TGG - chr4_2:11352965-11352984 None:intergenic 25.0%
! GCATGGTATAAAAATTTATG+AGG + chr4_2:11352975-11352994 Msa0588880:intron 25.0%
! TTGAGAAAAAATCTTGTTCT+TGG - chr4_2:11353258-11353277 None:intergenic 25.0%
TCTACCAACGTTAATTTAGA+TGG + chr4_2:11351729-11351748 Msa0588880:CDS 30.0%
CAAATAAAGCTTGTGGAATT+TGG + chr4_2:11351853-11351872 Msa0588880:intron 30.0%
GTTGTGTGAAAATCCTTTAA+GGG + chr4_2:11351882-11351901 Msa0588880:intron 30.0%
! AATTTTGTGTCAACCCTTAA+AGG - chr4_2:11351898-11351917 None:intergenic 30.0%
!! CATAATGTTTGATTGGTTTC+TGG + chr4_2:11352001-11352020 Msa0588880:intron 30.0%
!! TGATTGGTTTCTGGTATAAT+TGG + chr4_2:11352010-11352029 Msa0588880:intron 30.0%
! TGACAAGTTTTCTACAGAAA+AGG - chr4_2:11352149-11352168 None:intergenic 30.0%
! TGCTCTTTGATGCAATTTTT+AGG + chr4_2:11352426-11352445 Msa0588880:intron 30.0%
! GCTCTTTGATGCAATTTTTA+GGG + chr4_2:11352427-11352446 Msa0588880:intron 30.0%
!!! CAATTTAGTTTTGGGTTATG+TGG - chr4_2:11352956-11352975 None:intergenic 30.0%
TAACCCAAAACTAAATTGCA+TGG + chr4_2:11352958-11352977 Msa0588880:intron 30.0%
! GTAATTTTTGCAGTTTGTGA+TGG + chr4_2:11353080-11353099 Msa0588880:intron 30.0%
! TTTGTGATGGTTCTATTGAA+GGG + chr4_2:11353093-11353112 Msa0588880:CDS 30.0%
! ATATGGTGAACTAGTTTCAA+TGG + chr4_2:11353158-11353177 Msa0588880:CDS 30.0%
! TATGGTGAACTAGTTTCAAT+GGG + chr4_2:11353159-11353178 Msa0588880:CDS 30.0%
AAGAATCTAATCAGTACCAT+TGG + chr4_2:11353204-11353223 Msa0588880:CDS 30.0%
TCTACCAACGTTAATTTAGA+TGG + chr4_2:11351729-11351748 Msa0588880:CDS 30.0%
CAAATAAAGCTTGTGGAATT+TGG + chr4_2:11351853-11351872 Msa0588880:intron 30.0%
GTTGTGTGAAAATCCTTTAA+GGG + chr4_2:11351882-11351901 Msa0588880:intron 30.0%
! AATTTTGTGTCAACCCTTAA+AGG - chr4_2:11351898-11351917 None:intergenic 30.0%
!! CATAATGTTTGATTGGTTTC+TGG + chr4_2:11352001-11352020 Msa0588880:intron 30.0%
!! TGATTGGTTTCTGGTATAAT+TGG + chr4_2:11352010-11352029 Msa0588880:intron 30.0%
! TGACAAGTTTTCTACAGAAA+AGG - chr4_2:11352149-11352168 None:intergenic 30.0%
! TGCTCTTTGATGCAATTTTT+AGG + chr4_2:11352426-11352445 Msa0588880:intron 30.0%
! GCTCTTTGATGCAATTTTTA+GGG + chr4_2:11352427-11352446 Msa0588880:intron 30.0%
!!! CAATTTAGTTTTGGGTTATG+TGG - chr4_2:11352956-11352975 None:intergenic 30.0%
TAACCCAAAACTAAATTGCA+TGG + chr4_2:11352958-11352977 Msa0588880:intron 30.0%
! GTAATTTTTGCAGTTTGTGA+TGG + chr4_2:11353080-11353099 Msa0588880:intron 30.0%
! TTTGTGATGGTTCTATTGAA+GGG + chr4_2:11353093-11353112 Msa0588880:CDS 30.0%
! ATATGGTGAACTAGTTTCAA+TGG + chr4_2:11353158-11353177 Msa0588880:CDS 30.0%
! TATGGTGAACTAGTTTCAAT+GGG + chr4_2:11353159-11353178 Msa0588880:CDS 30.0%
AAGAATCTAATCAGTACCAT+TGG + chr4_2:11353204-11353223 Msa0588880:CDS 30.0%
! TAACGTTGGTAGAGATGTAA+AGG - chr4_2:11351722-11351741 None:intergenic 35.0%
!! GTTCTAGCTTGATCAAAGAT+AGG - chr4_2:11351768-11351787 None:intergenic 35.0%
! AGCTTGTGGAATTTGGTTTA+AGG + chr4_2:11351860-11351879 Msa0588880:intron 35.0%
GGTTGTGTGAAAATCCTTTA+AGG + chr4_2:11351881-11351900 Msa0588880:intron 35.0%
AGAAAAGGCAGAGAGTAAAA+GGG - chr4_2:11352134-11352153 None:intergenic 35.0%
AAATAAGACAGCAGACTAGT+TGG + chr4_2:11352365-11352384 Msa0588880:intron 35.0%
CTTGGAACAAAGTACAACAT+GGG - chr4_2:11352635-11352654 None:intergenic 35.0%
ACTTGGAACAAAGTACAACA+TGG - chr4_2:11352636-11352655 None:intergenic 35.0%
TCTTTGTGCGCAAAGTTAAT+AGG - chr4_2:11352735-11352754 None:intergenic 35.0%
! TTTGCGCACAAAGAATTTTG+CGG + chr4_2:11352741-11352760 Msa0588880:intron 35.0%
!!! CACAGTTTTGAGTACCAATT+TGG - chr4_2:11352781-11352800 None:intergenic 35.0%
! GTTTGTGATGGTTCTATTGA+AGG + chr4_2:11353092-11353111 Msa0588880:intron 35.0%
! TTGTGATGGTTCTATTGAAG+GGG + chr4_2:11353094-11353113 Msa0588880:CDS 35.0%
ATCTTGTTCTTGGTATAGAG+AGG - chr4_2:11353248-11353267 None:intergenic 35.0%
TCTCAAAGTCTTTGACATCA+TGG + chr4_2:11353272-11353291 Msa0588880:CDS 35.0%
! TAACGTTGGTAGAGATGTAA+AGG - chr4_2:11351722-11351741 None:intergenic 35.0%
!! GTTCTAGCTTGATCAAAGAT+AGG - chr4_2:11351768-11351787 None:intergenic 35.0%
! AGCTTGTGGAATTTGGTTTA+AGG + chr4_2:11351860-11351879 Msa0588880:intron 35.0%
GGTTGTGTGAAAATCCTTTA+AGG + chr4_2:11351881-11351900 Msa0588880:intron 35.0%
AGAAAAGGCAGAGAGTAAAA+GGG - chr4_2:11352134-11352153 None:intergenic 35.0%
AAATAAGACAGCAGACTAGT+TGG + chr4_2:11352365-11352384 Msa0588880:intron 35.0%
CTTGGAACAAAGTACAACAT+GGG - chr4_2:11352635-11352654 None:intergenic 35.0%
ACTTGGAACAAAGTACAACA+TGG - chr4_2:11352636-11352655 None:intergenic 35.0%
TCTTTGTGCGCAAAGTTAAT+AGG - chr4_2:11352735-11352754 None:intergenic 35.0%
! TTTGCGCACAAAGAATTTTG+CGG + chr4_2:11352741-11352760 Msa0588880:intron 35.0%
!!! CACAGTTTTGAGTACCAATT+TGG - chr4_2:11352781-11352800 None:intergenic 35.0%
! GTTTGTGATGGTTCTATTGA+AGG + chr4_2:11353092-11353111 Msa0588880:intron 35.0%
! TTGTGATGGTTCTATTGAAG+GGG + chr4_2:11353094-11353113 Msa0588880:CDS 35.0%
ATCTTGTTCTTGGTATAGAG+AGG - chr4_2:11353248-11353267 None:intergenic 35.0%
TCTCAAAGTCTTTGACATCA+TGG + chr4_2:11353272-11353291 Msa0588880:CDS 35.0%
TTGGTAGAGATGTAAAGGCA+AGG - chr4_2:11351717-11351736 None:intergenic 40.0%
!! ACTGCTGTTTCCACAATCAT+AGG + chr4_2:11351786-11351805 Msa0588880:CDS 40.0%
AATCATAGGAAAACCCGACA+AGG + chr4_2:11351800-11351819 Msa0588880:CDS 40.0%
AGAAGAGTGACATACCTTGT+CGG - chr4_2:11351817-11351836 None:intergenic 40.0%
GAAGTGTACACTTCTCTGTT+TGG + chr4_2:11352079-11352098 Msa0588880:intron 40.0%
CAGAAAAGGCAGAGAGTAAA+AGG - chr4_2:11352135-11352154 None:intergenic 40.0%
GATAACTTCTATGTGCTGGA+CGG - chr4_2:11352690-11352709 None:intergenic 40.0%
CCATGATAACTTCTATGTGC+TGG - chr4_2:11352694-11352713 None:intergenic 40.0%
CCAGCACATAGAAGTTATCA+TGG + chr4_2:11352691-11352710 Msa0588880:intron 40.0%
! CGATGTGATAGTCTTAGAGT+TGG + chr4_2:11353025-11353044 Msa0588880:intron 40.0%
AACAAAGAACCAGCTGCATA+TGG + chr4_2:11353141-11353160 Msa0588880:CDS 40.0%
CAGTACCATTGGCACAATAT+TGG + chr4_2:11353215-11353234 Msa0588880:CDS 40.0%
GGATTCCAATATTGTGCCAA+TGG - chr4_2:11353223-11353242 None:intergenic 40.0%
TGTTCTTGGTATAGAGAGGT+TGG - chr4_2:11353244-11353263 None:intergenic 40.0%
TTGGTAGAGATGTAAAGGCA+AGG - chr4_2:11351717-11351736 None:intergenic 40.0%
!! ACTGCTGTTTCCACAATCAT+AGG + chr4_2:11351786-11351805 Msa0588880:CDS 40.0%
AATCATAGGAAAACCCGACA+AGG + chr4_2:11351800-11351819 Msa0588880:CDS 40.0%
AGAAGAGTGACATACCTTGT+CGG - chr4_2:11351817-11351836 None:intergenic 40.0%
GAAGTGTACACTTCTCTGTT+TGG + chr4_2:11352079-11352098 Msa0588880:intron 40.0%
CAGAAAAGGCAGAGAGTAAA+AGG - chr4_2:11352135-11352154 None:intergenic 40.0%
GATAACTTCTATGTGCTGGA+CGG - chr4_2:11352690-11352709 None:intergenic 40.0%
CCATGATAACTTCTATGTGC+TGG - chr4_2:11352694-11352713 None:intergenic 40.0%
CCAGCACATAGAAGTTATCA+TGG + chr4_2:11352691-11352710 Msa0588880:intron 40.0%
! CGATGTGATAGTCTTAGAGT+TGG + chr4_2:11353025-11353044 Msa0588880:intron 40.0%
AACAAAGAACCAGCTGCATA+TGG + chr4_2:11353141-11353160 Msa0588880:CDS 40.0%
CAGTACCATTGGCACAATAT+TGG + chr4_2:11353215-11353234 Msa0588880:CDS 40.0%
GGATTCCAATATTGTGCCAA+TGG - chr4_2:11353223-11353242 None:intergenic 40.0%
TGTTCTTGGTATAGAGAGGT+TGG - chr4_2:11353244-11353263 None:intergenic 40.0%
! GTCGGGTTTTCCTATGATTG+TGG - chr4_2:11351799-11351818 None:intergenic 45.0%
GAAGAGTGACATACCTTGTC+GGG - chr4_2:11351816-11351835 None:intergenic 45.0%
! TAAGACAGCAGACTAGTTGG+AGG + chr4_2:11352368-11352387 Msa0588880:intron 45.0%
! TTGGAGGCTTTTCTGTCTGT+AGG + chr4_2:11352384-11352403 Msa0588880:intron 45.0%
GGGTTATGTGGCATTGTTGT+AGG - chr4_2:11352944-11352963 None:intergenic 45.0%
ACTAGTTCACCATATGCAGC+TGG - chr4_2:11353153-11353172 None:intergenic 45.0%
! GGTGAACTAGTTTCAATGGG+TGG + chr4_2:11353162-11353181 Msa0588880:CDS 45.0%
! GTCGGGTTTTCCTATGATTG+TGG - chr4_2:11351799-11351818 None:intergenic 45.0%
GAAGAGTGACATACCTTGTC+GGG - chr4_2:11351816-11351835 None:intergenic 45.0%
! TAAGACAGCAGACTAGTTGG+AGG + chr4_2:11352368-11352387 Msa0588880:intron 45.0%
! TTGGAGGCTTTTCTGTCTGT+AGG + chr4_2:11352384-11352403 Msa0588880:intron 45.0%
GGGTTATGTGGCATTGTTGT+AGG - chr4_2:11352944-11352963 None:intergenic 45.0%
ACTAGTTCACCATATGCAGC+TGG - chr4_2:11353153-11353172 None:intergenic 45.0%
! GGTGAACTAGTTTCAATGGG+TGG + chr4_2:11353162-11353181 Msa0588880:CDS 45.0%
!!! TTTTTATTGTATAAATATAA+TGG + chr4_2:11352292-11352311 Msa0588880:intron 5.0%
!!! TTTTTATTGTATAAATATAA+TGG + chr4_2:11352292-11352311 Msa0588880:intron 5.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 11351711 11353326 11351711 ID=Msa0588880;Name=Msa0588880
chr4_2 mRNA 11351711 11353326 11351711 ID=Msa0588880-mRNA-1;Parent=Msa0588880;Name=Msa0588880-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|1|0|1|1|1|2|0|114
chr4_2 exon 11351711 11351821 11351711 ID=Msa0588880-mRNA-1:exon:3289;Parent=Msa0588880-mRNA-1
chr4_2 exon 11353093 11353326 11353093 ID=Msa0588880-mRNA-1:exon:3290;Parent=Msa0588880-mRNA-1
chr4_2 CDS 11351711 11351821 11351711 ID=Msa0588880-mRNA-1:cds;Parent=Msa0588880-mRNA-1
chr4_2 CDS 11353093 11353326 11353093 ID=Msa0588880-mRNA-1:cds;Parent=Msa0588880-mRNA-1
Gene Sequence

>Msa0588880

ATGCCTTGCCTTTACATCTCTACCAACGTTAATTTAGATGGAAAAAACATCGATCCTATCTTTGATCAAGCTAGAACTGCTGTTTCCACAATCATAGGAAAACCCGACAAGTTTGTGATGGTTCTATTGAAGGGGTCAGTTCCAATATCGTTTGAAAATAACAAAGAACCAGCTGCATATGGTGAACTAGTTTCAATGGGTGGCATTAATTCAGAAGTGAAGAAGAATCTAATCAGTACCATTGGCACAATATTGGAATCCAACCTCTCTATACCAAGAACAAGATTTTTTCTCAAAGTCTTTGACATCATGGTTCTCAAAATTGAAAATAGCTCCAAACTGTAG

Protein sequence

>Msa0588880

MPCLYISTNVNLDGKNIDPIFDQARTAVSTIIGKPDKFVMVLLKGSVPISFENNKEPAAYGELVSMGGINSEVKKNLISTIGTILESNLSIPRTRFFLKVFDIMVLKIENSSKL*