AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0201920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0201920 MtrunA17_Chr2g0298071 95.331 257 7 3 1 256 1 253 0.0 499
Msa0201920 MtrunA17_Chr4g0072101 39.303 201 80 4 57 256 4 163 7.97e-32 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0201920 AT5G57340.1 34.545 275 118 10 1 255 1 233 1.25e-21 90.5
Msa0201920 AT5G57340.2 33.455 275 121 10 1 255 19 251 2.48e-21 90.1

Find 65 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATAGTATTAAACTTTA+TGG 0.093335 2_1:-23872930 Msa0201920:five_prime_UTR
ATAATTCCTCTTTCAAAATT+TGG 0.105021 2_1:+23870246 None:intergenic
GTGGATGATTAATATCTTTA+TGG 0.143012 2_1:+23872973 None:intergenic
AAGGCAAGCAAAATAAATTT+TGG 0.180508 2_1:-23870349 Msa0201920:CDS
TACATTTGATCAAGATAAAA+AGG 0.235098 2_1:-23869943 Msa0201920:CDS
TTTACTTTATTCCAATAAAA+TGG 0.251455 2_1:-23869763 Msa0201920:three_prime_UTR
GGACCACTCAATTTATTATA+TGG 0.252972 2_1:-23869820 Msa0201920:three_prime_UTR
CTTGGCAGTTCATATTCTTT+TGG 0.254987 2_1:+23873093 None:intergenic
TGTTGTTGAGGTAATAATTT+AGG 0.265185 2_1:+23873179 None:intergenic
GAAAGAAGTGGAAGTTGTTC+AGG 0.278248 2_1:-23870215 Msa0201920:CDS
AGTAACAAACATTGCAATAT+TGG 0.285715 2_1:-23871098 Msa0201920:CDS
TATCTGCATTGTCTTTCTAA+TGG 0.285749 2_1:+23871019 None:intergenic
TGAGTATGCTAGAGGATTTA+TGG 0.299297 2_1:+23871193 None:intergenic
TCATATTCTTGAGATGCTTT+AGG 0.334440 2_1:+23873430 None:intergenic
ATACCTATGCTACCATTCAT+AGG 0.347754 2_1:+23870323 None:intergenic
GAAAGAGGAATTATACATAA+AGG 0.351691 2_1:-23870237 Msa0201920:CDS
GGTCCATGTTTAGTGTAAAT+AGG 0.365817 2_1:+23869838 None:intergenic
CACTTGTGTTATATTAGTAT+TGG 0.386558 2_1:-23872953 Msa0201920:five_prime_UTR
TCACAATCACTTGCTGCTGC+TGG 0.396014 2_1:+23870149 None:intergenic
TCTTCAAATTCTTCAGTGCT+TGG 0.405096 2_1:-23873046 Msa0201920:five_prime_UTR
GTGGATTTGACTTTATGTAT+AGG 0.411271 2_1:-23870113 Msa0201920:CDS
GAGCTGCTCATTGGTGTAGT+GGG 0.415795 2_1:+23869972 None:intergenic
GTGTGGTTGGTGTTGATGCC+TGG 0.418366 2_1:-23871151 Msa0201920:CDS
GGTAATAATTTAGGTTTGAC+AGG 0.427224 2_1:+23873188 None:intergenic
CTGTCAAGTATCCTATCCAT+TGG 0.443227 2_1:+23871056 None:intergenic
GAAGGAGGCAAGGCAAAAGC+TGG 0.453555 2_1:+23873219 None:intergenic
TTTGTTGAACAAAATAAGGT+TGG 0.453849 2_1:+23870297 None:intergenic
CTCAAGAATATGAATACAAT+GGG 0.453958 2_1:-23873420 Msa0201920:five_prime_UTR
TACCTATGCTACCATTCATA+GGG 0.460339 2_1:+23870324 None:intergenic
AAGTCTTATATGCTTCCAAA+TGG 0.472702 2_1:-23870062 Msa0201920:CDS
GCACTGAAGAATTTGAAGAT+AGG 0.487276 2_1:+23873050 None:intergenic
AGAGCTGCTCATTGGTGTAG+TGG 0.503631 2_1:+23869971 None:intergenic
TGAATACAATGGGATGTGAT+TGG 0.509342 2_1:-23873410 Msa0201920:five_prime_UTR
CCTCTTTCAAAATTTGGACA+TGG 0.511793 2_1:+23870252 None:intergenic
AAGTGATTGTGATGAAGATA+TGG 0.519183 2_1:-23870138 Msa0201920:CDS
TCCAAATGGAATGAAAACTA+AGG 0.522393 2_1:-23870048 Msa0201920:CDS
GTAACACTAGAGCTGCTCAT+TGG 0.525161 2_1:+23869963 None:intergenic
ATTCCTATTTACACTAAACA+TGG 0.531539 2_1:-23869841 Msa0201920:three_prime_UTR
TGGCTAAATGAAATTTGTGT+TGG 0.533437 2_1:+23870272 None:intergenic
TCTCAAGAATATGAATACAA+TGG 0.542189 2_1:-23873421 Msa0201920:five_prime_UTR
TAATAACTTGCAAAATCCAA+TGG 0.546440 2_1:-23871072 Msa0201920:CDS
ACTTGCAAAATCCAATGGAT+AGG 0.552356 2_1:-23871067 Msa0201920:CDS
GGGCCACATTGGCTTTCTCA+AGG 0.553022 2_1:-23869921 Msa0201920:CDS
CAATACTAATATAACACAAG+TGG 0.553056 2_1:+23872954 None:intergenic
GGCTGTGAAGGAAGGAGGCA+AGG 0.558019 2_1:+23873209 None:intergenic
GTTTGACAGGCTGTGAAGGA+AGG 0.558750 2_1:+23873201 None:intergenic
TTGTGATGAAGATATGGAAG+TGG 0.570304 2_1:-23870132 Msa0201920:CDS
TTTGTTCTGATAATGCTGCC+AGG 0.573507 2_1:+23871133 None:intergenic
CTGGAATAATGATGTTGTTG+AGG 0.583711 2_1:+23873167 None:intergenic
AAACATCAACAAAGCAACCT+AGG 0.585277 2_1:+23873120 None:intergenic
TGCAACAACTTCAGTGTGGT+TGG 0.585657 2_1:-23871164 Msa0201920:CDS
TTAGGTTTGACAGGCTGTGA+AGG 0.588272 2_1:+23873197 None:intergenic
AGCTGCTCATTGGTGTAGTG+GGG 0.600539 2_1:+23869973 None:intergenic
AAAGACAATGCAGATACAAG+AGG 0.602336 2_1:-23871012 Msa0201920:CDS
TTATACATAAAGGAAAGAAG+TGG 0.603251 2_1:-23870227 Msa0201920:CDS
CAATCTACTGAGTATGCTAG+AGG 0.610316 2_1:+23871185 None:intergenic
TGACAGGCTGTGAAGGAAGG+AGG 0.624890 2_1:+23873204 None:intergenic
GAACCATATAATAAATTGAG+TGG 0.626635 2_1:+23869817 None:intergenic
ATAGACAGCATGAAGACACT+TGG 0.628877 2_1:+23873075 None:intergenic
ACCCCTATGAATGGTAGCAT+AGG 0.633561 2_1:-23870326 Msa0201920:CDS
TGGCAGCATTATCAGAACAA+AGG 0.642212 2_1:-23871131 Msa0201920:CDS
AAACCTTGAGAAAGCCAATG+TGG 0.666219 2_1:+23869918 None:intergenic
ACCTATGCTACCATTCATAG+GGG 0.677238 2_1:+23870325 None:intergenic
GATACTTGACAGAAACAACA+TGG 0.687441 2_1:-23871045 Msa0201920:CDS
AGATTGCAACAACTTCAGTG+TGG 0.741905 2_1:-23871168 Msa0201920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAATAAAAGGA+CGG + chr2_1:23870072-23870091 None:intergenic 10.0%
!! TTGGAAAAAATAAAAATAAA+AGG + chr2_1:23870076-23870095 None:intergenic 10.0%
!! TTTAATAGTATTAAACTTTA+TGG - chr2_1:23870156-23870175 Msa0201920:CDS 10.0%
!! TTGAAGAATAATAATAAAAT+AGG + chr2_1:23871043-23871062 None:intergenic 10.0%
!! AATTGAAAAAAATAAAATGA+TGG + chr2_1:23871465-23871484 None:intergenic 10.0%
!!! TTGTAAGTTTTTTATTTTTT+CGG - chr2_1:23871798-23871817 Msa0201920:intron 10.0%
!! ATTAGTATTTGAAAATTTAT+AGG - chr2_1:23872654-23872673 Msa0201920:intron 10.0%
!!! AAAAGAATTTTTTTCTTACA+TGG - chr2_1:23870455-23870474 Msa0201920:intron 15.0%
!! TTCTATTAATATTTAGTGTT+TGG - chr2_1:23871201-23871220 Msa0201920:CDS 15.0%
!!! CAAATTTTAATTGTATTAGT+TGG - chr2_1:23872214-23872233 Msa0201920:intron 15.0%
!! CAAATGTAAAAAAAAATTGT+TGG - chr2_1:23872299-23872318 Msa0201920:intron 15.0%
!! TTTGTACAATTTATTCAATT+GGG + chr2_1:23872514-23872533 None:intergenic 15.0%
!!! TTTTGTACAATTTATTCAAT+TGG + chr2_1:23872515-23872534 None:intergenic 15.0%
!! ATTTGAAAATTTATAGGTAA+AGG - chr2_1:23872660-23872679 Msa0201920:intron 15.0%
!! TTTACTTTATTCCAATAAAA+TGG - chr2_1:23873323-23873342 Msa0201920:five_prime_UTR 15.0%
!!! ATATAAGTTATCCATTTTAT+TGG + chr2_1:23873337-23873356 None:intergenic 15.0%
!! ATAAAAAAAATTGTTCACCT+AGG - chr2_1:23869949-23869968 Msa0201920:CDS 20.0%
!! AACTTTATGGATAAAAATGT+TGG - chr2_1:23870169-23870188 Msa0201920:CDS 20.0%
!! AAGGATTATGAATTATGATT+AGG + chr2_1:23870302-23870321 None:intergenic 20.0%
!! ATACTCAAGAAAAGAAAAAA+AGG + chr2_1:23870321-23870340 None:intergenic 20.0%
!! TTAAGTGTATATATATGTGA+AGG - chr2_1:23870628-23870647 Msa0201920:intron 20.0%
!! TAAGTGTATATATATGTGAA+GGG - chr2_1:23870629-23870648 Msa0201920:intron 20.0%
!!! AAGATATTATTTTCAGATCA+TGG - chr2_1:23871134-23871153 Msa0201920:CDS 20.0%
!! GTCAAAATATTCTTAGAATA+AGG - chr2_1:23871177-23871196 Msa0201920:CDS 20.0%
!! TATGTTATTAAGTTAGTTAG+AGG + chr2_1:23871551-23871570 None:intergenic 20.0%
!! TAAACTAGATATTGACAATT+TGG + chr2_1:23871739-23871758 None:intergenic 20.0%
!!! GCTTCTTTAGTTTTAAATTA+AGG - chr2_1:23872236-23872255 Msa0201920:intron 20.0%
!!! TGCACAAGTATTAAAAATTT+TGG - chr2_1:23872367-23872386 Msa0201920:intron 20.0%
!!! GCACAAGTATTAAAAATTTT+GGG - chr2_1:23872368-23872387 Msa0201920:intron 20.0%
!! TACCCTTAAATTAAATGATT+TGG - chr2_1:23872427-23872446 Msa0201920:intron 20.0%
!! ACCCTTAAATTAAATGATTT+GGG - chr2_1:23872428-23872447 Msa0201920:intron 20.0%
!!! ACCCAAATCATTTAATTTAA+GGG + chr2_1:23872432-23872451 None:intergenic 20.0%
!!! AACCCAAATCATTTAATTTA+AGG + chr2_1:23872433-23872452 None:intergenic 20.0%
!! TTGTACAATTTATTCAATTG+GGG + chr2_1:23872513-23872532 None:intergenic 20.0%
!! ATAATTCCTCTTTCAAAATT+TGG + chr2_1:23872843-23872862 None:intergenic 20.0%
!! TACATTTGATCAAGATAAAA+AGG - chr2_1:23873143-23873162 Msa0201920:five_prime_UTR 20.0%
!! ACATTTGATCAAGATAAAAA+GGG - chr2_1:23873144-23873163 Msa0201920:five_prime_UTR 20.0%
!! ATAAAGTAAAAAACTCATCT+AGG + chr2_1:23873313-23873332 None:intergenic 20.0%
! TCTCAAGAATATGAATACAA+TGG - chr2_1:23869665-23869684 Msa0201920:three_prime_UTR 25.0%
! CTCAAGAATATGAATACAAT+GGG - chr2_1:23869666-23869685 Msa0201920:three_prime_UTR 25.0%
! TGTTGTTGAGGTAATAATTT+AGG + chr2_1:23869910-23869929 None:intergenic 25.0%
! GTGGATGATTAATATCTTTA+TGG + chr2_1:23870116-23870135 None:intergenic 25.0%
! CAATACTAATATAACACAAG+TGG + chr2_1:23870135-23870154 None:intergenic 25.0%
! CACTTGTGTTATATTAGTAT+TGG - chr2_1:23870133-23870152 Msa0201920:CDS 25.0%
! TACTAAACCAAAAATAGAAG+AGG + chr2_1:23870970-23870989 None:intergenic 25.0%
! TTGAATCTATGCATACAAAA+AGG - chr2_1:23871094-23871113 Msa0201920:CDS 25.0%
!!! TAAGTATTGATTTGCATTTC+AGG - chr2_1:23871847-23871866 Msa0201920:intron 25.0%
! AGTAACAAACATTGCAATAT+TGG - chr2_1:23871988-23872007 Msa0201920:intron 25.0%
!! TAATAACTTGCAAAATCCAA+TGG - chr2_1:23872014-23872033 Msa0201920:intron 25.0%
! TTGCTTAACATATGTCTTTA+GGG - chr2_1:23872391-23872410 Msa0201920:intron 25.0%
!!! ATTAAATGATTTGGGTTGAA+TGG - chr2_1:23872436-23872455 Msa0201920:intron 25.0%
! GCATCAAATAAAAACATAGT+TGG + chr2_1:23872588-23872607 None:intergenic 25.0%
!! AAGGCAAGCAAAATAAATTT+TGG - chr2_1:23872737-23872756 Msa0201920:intron 25.0%
! TTTGTTGAACAAAATAAGGT+TGG + chr2_1:23872792-23872811 None:intergenic 25.0%
!! GGTTTTTGTTGAACAAAATA+AGG + chr2_1:23872796-23872815 None:intergenic 25.0%
! GAAAGAGGAATTATACATAA+AGG - chr2_1:23872849-23872868 Msa0201920:five_prime_UTR 25.0%
! TTATACATAAAGGAAAGAAG+TGG - chr2_1:23872859-23872878 Msa0201920:five_prime_UTR 25.0%
!! TCTTTTCAATCAGATAGAAT+TGG - chr2_1:23873078-23873097 Msa0201920:five_prime_UTR 25.0%
! CATTTGATCAAGATAAAAAG+GGG - chr2_1:23873145-23873164 Msa0201920:five_prime_UTR 25.0%
! ATTCCTATTTACACTAAACA+TGG - chr2_1:23873245-23873264 Msa0201920:five_prime_UTR 25.0%
! GAACCATATAATAAATTGAG+TGG + chr2_1:23873272-23873291 None:intergenic 25.0%
TCATATTCTTGAGATGCTTT+AGG + chr2_1:23869659-23869678 None:intergenic 30.0%
! GGTAATAATTTAGGTTTGAC+AGG + chr2_1:23869901-23869920 None:intergenic 30.0%
!!! TTTTTTCCAACCATGATTCA+AGG - chr2_1:23870086-23870105 Msa0201920:CDS 30.0%
! TTATGGTTTTCCTTGAATCA+TGG + chr2_1:23870099-23870118 None:intergenic 30.0%
!! ATTTTGCTACTTCTTACAAC+AGG - chr2_1:23870238-23870257 Msa0201920:CDS 30.0%
TCTTCACTCAAATAACATGA+AGG - chr2_1:23870348-23870367 Msa0201920:CDS 30.0%
!! TGCATAGTTTCTAGTAATAG+TGG - chr2_1:23870802-23870821 Msa0201920:intron 30.0%
TCATTCATCATCAAAGTTTC+TGG - chr2_1:23870888-23870907 Msa0201920:intron 30.0%
! ACTAGTTCCTCTTCTATTTT+TGG - chr2_1:23870960-23870979 Msa0201920:intron 30.0%
GAGTAAATTTGTCAAAACAG+AGG + chr2_1:23870999-23871018 None:intergenic 30.0%
GTTAGTACAGTTGCTATATA+TGG - chr2_1:23871070-23871089 Msa0201920:CDS 30.0%
ATGGTTTGAATTTCTGTTGT+TGG - chr2_1:23871153-23871172 Msa0201920:CDS 30.0%
TCTTAAGCACAAACAGAAAA+GGG - chr2_1:23871279-23871298 Msa0201920:intron 30.0%
TAGAGACTAACAAGTCTAAT+AGG + chr2_1:23871328-23871347 None:intergenic 30.0%
TGGAATATATCTACGTATGT+TGG + chr2_1:23871445-23871464 None:intergenic 30.0%
!! ATTGATTTGCATTTCAGGAA+TGG - chr2_1:23871852-23871871 Msa0201920:intron 30.0%
!! TTGATTTGCATTTCAGGAAT+GGG - chr2_1:23871853-23871872 Msa0201920:intron 30.0%
TATCTGCATTGTCTTTCTAA+TGG + chr2_1:23872070-23872089 None:intergenic 30.0%
TCCATTCAATTTGTACCATA+AGG - chr2_1:23872158-23872177 Msa0201920:intron 30.0%
ACCTTATGGTACAAATTGAA+TGG + chr2_1:23872162-23872181 None:intergenic 30.0%
! ATAGCATGACCAATTTTCAT+AGG - chr2_1:23872185-23872204 Msa0201920:intron 30.0%
TTGATCCATCCTATGAAAAT+TGG + chr2_1:23872197-23872216 None:intergenic 30.0%
CTCGTTAACTTATGTCTTAA+GGG + chr2_1:23872278-23872297 None:intergenic 30.0%
ACTCGTTAACTTATGTCTTA+AGG + chr2_1:23872279-23872298 None:intergenic 30.0%
GTTGCTTAACATATGTCTTT+AGG - chr2_1:23872390-23872409 Msa0201920:intron 30.0%
AATGAGAATAATGGCTTTCT+TGG + chr2_1:23872469-23872488 None:intergenic 30.0%
GTTTGGCATAATGAGAATAA+TGG + chr2_1:23872478-23872497 None:intergenic 30.0%
! CATAGTTGGTATATGACAAT+AGG + chr2_1:23872574-23872593 None:intergenic 30.0%
CAATGTGCAAAAAATGCTAA+TGG - chr2_1:23872699-23872718 Msa0201920:intron 30.0%
ATGGATGAACACAAAAATGA+AGG - chr2_1:23872718-23872737 Msa0201920:intron 30.0%
TGGCTAAATGAAATTTGTGT+TGG + chr2_1:23872817-23872836 None:intergenic 30.0%
AAGTGATTGTGATGAAGATA+TGG - chr2_1:23872948-23872967 Msa0201920:five_prime_UTR 30.0%
! GTGGATTTGACTTTATGTAT+AGG - chr2_1:23872973-23872992 Msa0201920:five_prime_UTR 30.0%
! AAGTCTTATATGCTTCCAAA+TGG - chr2_1:23873024-23873043 Msa0201920:five_prime_UTR 30.0%
TCCAAATGGAATGAAAACTA+AGG - chr2_1:23873038-23873057 Msa0201920:five_prime_UTR 30.0%
!! TCCTTAGTTTTCATTCCATT+TGG + chr2_1:23873042-23873061 None:intergenic 30.0%
GGACCACTCAATTTATTATA+TGG - chr2_1:23873266-23873285 Msa0201920:five_prime_UTR 30.0%
TGAATACAATGGGATGTGAT+TGG - chr2_1:23869676-23869695 Msa0201920:three_prime_UTR 35.0%
CTGGAATAATGATGTTGTTG+AGG + chr2_1:23869922-23869941 None:intergenic 35.0%
AAACATCAACAAAGCAACCT+AGG + chr2_1:23869969-23869988 None:intergenic 35.0%
! CTTGGCAGTTCATATTCTTT+TGG + chr2_1:23869996-23870015 None:intergenic 35.0%
GCACTGAAGAATTTGAAGAT+AGG + chr2_1:23870039-23870058 None:intergenic 35.0%
TCTTCAAATTCTTCAGTGCT+TGG - chr2_1:23870040-23870059 Msa0201920:CDS 35.0%
TAAGCTTAACCTATGCAACA+CGG - chr2_1:23870493-23870512 Msa0201920:intron 35.0%
GATGAATGAACATTATGTGC+AGG + chr2_1:23870877-23870896 None:intergenic 35.0%
CTCTTAAGCACAAACAGAAA+AGG - chr2_1:23871278-23871297 Msa0201920:intron 35.0%
GATCACAAGACTTTAAGTGT+AGG + chr2_1:23871636-23871655 None:intergenic 35.0%
CTTCAATCAGATTGAAGTGT+AGG + chr2_1:23871660-23871679 None:intergenic 35.0%
AACATATGTCGATGTCATGT+CGG + chr2_1:23871702-23871721 None:intergenic 35.0%
TGAGTATGCTAGAGGATTTA+TGG + chr2_1:23871896-23871915 None:intergenic 35.0%
ACTTGCAAAATCCAATGGAT+AGG - chr2_1:23872019-23872038 Msa0201920:intron 35.0%
GATACTTGACAGAAACAACA+TGG - chr2_1:23872041-23872060 Msa0201920:intron 35.0%
AAAGACAATGCAGATACAAG+AGG - chr2_1:23872074-23872093 Msa0201920:intron 35.0%
AGAGGATATCTTCAAAAACC+AGG - chr2_1:23872092-23872111 Msa0201920:intron 35.0%
TACTACATTGACATGATACC+TGG + chr2_1:23872113-23872132 None:intergenic 35.0%
! CATGACCAATTTTCATAGGA+TGG - chr2_1:23872189-23872208 Msa0201920:intron 35.0%
TTATTCAATTGGGGACTTGA+AGG + chr2_1:23872504-23872523 None:intergenic 35.0%
! AAATTTTGGACCCCTATGAA+TGG - chr2_1:23872751-23872770 Msa0201920:intron 35.0%
TACCTATGCTACCATTCATA+GGG + chr2_1:23872765-23872784 None:intergenic 35.0%
ATACCTATGCTACCATTCAT+AGG + chr2_1:23872766-23872785 None:intergenic 35.0%
CCTCTTTCAAAATTTGGACA+TGG + chr2_1:23872837-23872856 None:intergenic 35.0%
!! CCATGTCCAAATTTTGAAAG+AGG - chr2_1:23872834-23872853 Msa0201920:intron 35.0%
TTGTGATGAAGATATGGAAG+TGG - chr2_1:23872954-23872973 Msa0201920:five_prime_UTR 35.0%
GGTCCATGTTTAGTGTAAAT+AGG + chr2_1:23873251-23873270 None:intergenic 35.0%
ATAGACAGCATGAAGACACT+TGG + chr2_1:23870014-23870033 None:intergenic 40.0%
! GGTTTTCCTTGAATCATGGT+TGG + chr2_1:23870095-23870114 None:intergenic 40.0%
AACACGGACACTTCTCATAT+TGG - chr2_1:23870509-23870528 Msa0201920:intron 40.0%
ACACGGACACTTCTCATATT+GGG - chr2_1:23870510-23870529 Msa0201920:intron 40.0%
CACTTAAGCTCTATGAAGCA+TGG + chr2_1:23870615-23870634 None:intergenic 40.0%
GTGGATGAAGACTACTGTAA+TGG - chr2_1:23870821-23870840 Msa0201920:intron 40.0%
! TGTGTTTCTGTCACAAGACT+AGG + chr2_1:23870848-23870867 None:intergenic 40.0%
! ACGTCTTCTATCTGAAGTGT+CGG + chr2_1:23871390-23871409 None:intergenic 40.0%
CTTCAGATAGAAGACGTGTA+TGG - chr2_1:23871393-23871412 Msa0201920:intron 40.0%
GTATGGTGACTAACACGTAT+TGG - chr2_1:23871410-23871429 Msa0201920:intron 40.0%
! TGACTAACACGTATTGGTGT+TGG - chr2_1:23871416-23871435 Msa0201920:intron 40.0%
! AAGTTAGTTAGAGGAAGCAC+CGG + chr2_1:23871542-23871561 None:intergenic 40.0%
AGTGTAGGAGATTGAAGTGT+AGG + chr2_1:23871621-23871640 None:intergenic 40.0%
AATCTGATTGAAGTCGTGTC+TGG - chr2_1:23871667-23871686 Msa0201920:intron 40.0%
CAATCTACTGAGTATGCTAG+AGG + chr2_1:23871904-23871923 None:intergenic 40.0%
AGATTGCAACAACTTCAGTG+TGG - chr2_1:23871918-23871937 Msa0201920:intron 40.0%
! TTTGTTCTGATAATGCTGCC+AGG + chr2_1:23871956-23871975 None:intergenic 40.0%
TGGCAGCATTATCAGAACAA+AGG - chr2_1:23871955-23871974 Msa0201920:intron 40.0%
CTGTCAAGTATCCTATCCAT+TGG + chr2_1:23872033-23872052 None:intergenic 40.0%
ACCTATGCTACCATTCATAG+GGG + chr2_1:23872764-23872783 None:intergenic 40.0%
GAAAGAAGTGGAAGTTGTTC+AGG - chr2_1:23872871-23872890 Msa0201920:five_prime_UTR 40.0%
AAGATAAAAAGGGGCCACAT+TGG - chr2_1:23873154-23873173 Msa0201920:five_prime_UTR 40.0%
AAACCTTGAGAAAGCCAATG+TGG + chr2_1:23873171-23873190 None:intergenic 40.0%
TTTGTGTGAGAGAGAGAGAG+AGG + chr2_1:23869806-23869825 None:intergenic 45.0%
TTAGGTTTGACAGGCTGTGA+AGG + chr2_1:23869892-23869911 None:intergenic 45.0%
CACGGACACTTCTCATATTG+GGG - chr2_1:23870511-23870530 Msa0201920:intron 45.0%
GTCAGTGTATGTGTTGTGTC+TGG - chr2_1:23870574-23870593 Msa0201920:intron 45.0%
TCCAATATCCGTGTCTTTGC+CGG - chr2_1:23871520-23871539 Msa0201920:intron 45.0%
TGCAACAACTTCAGTGTGGT+TGG - chr2_1:23871922-23871941 Msa0201920:intron 45.0%
GGTCATGCTATGCAACCTTA+TGG + chr2_1:23872176-23872195 None:intergenic 45.0%
ACCCCTATGAATGGTAGCAT+AGG - chr2_1:23872760-23872779 Msa0201920:intron 45.0%
GTAACACTAGAGCTGCTCAT+TGG + chr2_1:23873126-23873145 None:intergenic 45.0%
!!! AATTTTTTTTATATAAAAAC+TGG + chr2_1:23869941-23869960 None:intergenic 5.0%
!!! TTATTTTTTTCAATTATTAT+TGG - chr2_1:23871470-23871489 Msa0201920:intron 5.0%
GTTTGACAGGCTGTGAAGGA+AGG + chr2_1:23869888-23869907 None:intergenic 50.0%
!! GAGAAGTGTCCGTGTTGCAT+AGG + chr2_1:23870505-23870524 None:intergenic 50.0%
! CTTCTCATATTGGGGTGTCC+TGG - chr2_1:23870519-23870538 Msa0201920:intron 50.0%
! ATATTGGGGTGTCCTGGTGT+CGG - chr2_1:23870525-23870544 Msa0201920:intron 50.0%
ATACACTGACATGCCGACAC+CGG + chr2_1:23870564-23870583 None:intergenic 50.0%
ACCGGCAAAGACACGGATAT+TGG + chr2_1:23871524-23871543 None:intergenic 50.0%
!! TGGGGACTTGAAGGAGTGTT+TGG + chr2_1:23872495-23872514 None:intergenic 50.0%
! TCACAATCACTTGCTGCTGC+TGG + chr2_1:23872940-23872959 None:intergenic 50.0%
AGCTGCTCATTGGTGTAGTG+GGG + chr2_1:23873116-23873135 None:intergenic 50.0%
GAGCTGCTCATTGGTGTAGT+GGG + chr2_1:23873117-23873136 None:intergenic 50.0%
AGAGCTGCTCATTGGTGTAG+TGG + chr2_1:23873118-23873137 None:intergenic 50.0%
GAAGGAGGCAAGGCAAAAGC+TGG + chr2_1:23869870-23869889 None:intergenic 55.0%
TGACAGGCTGTGAAGGAAGG+AGG + chr2_1:23869885-23869904 None:intergenic 55.0%
TGTCGGATACGTGTCGTGTC+CGG - chr2_1:23870542-23870561 Msa0201920:intron 55.0%
ATACGTGTCGTGTCCGGTGT+CGG - chr2_1:23870548-23870567 Msa0201920:intron 55.0%
!! AGGAAGCACCGGCAAAGACA+CGG + chr2_1:23871531-23871550 None:intergenic 55.0%
!! GTGTGGTTGGTGTTGATGCC+TGG - chr2_1:23871935-23871954 Msa0201920:intron 55.0%
!! GGGCCACATTGGCTTTCTCA+AGG - chr2_1:23873165-23873184 Msa0201920:five_prime_UTR 55.0%
GGCTGTGAAGGAAGGAGGCA+AGG + chr2_1:23869880-23869899 None:intergenic 60.0%
CACGACACGTATCCGACACC+AGG + chr2_1:23870540-23870559 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 23869649 23873459 23869649 ID=Msa0201920;Name=Msa0201920
chr2_1 mRNA 23869649 23873459 23869649 ID=Msa0201920-mRNA-1;Parent=Msa0201920;Name=Msa0201920-mRNA-1;_AED=0.03;_eAED=0.03;_QI=613|1|1|1|0.5|0.66|3|256|256
chr2_1 exon 23869649 23870432 23869649 ID=Msa0201920-mRNA-1:exon:9197;Parent=Msa0201920-mRNA-1
chr2_1 exon 23870995 23871239 23870995 ID=Msa0201920-mRNA-1:exon:9196;Parent=Msa0201920-mRNA-1
chr2_1 exon 23872849 23873459 23872849 ID=Msa0201920-mRNA-1:exon:9195;Parent=Msa0201920-mRNA-1
chr2_1 five_prime_UTR 23872849 23873459 23872849 ID=Msa0201920-mRNA-1:five_prime_utr;Parent=Msa0201920-mRNA-1
chr2_1 five_prime_UTR 23871238 23871239 23871238 ID=Msa0201920-mRNA-1:five_prime_utr;Parent=Msa0201920-mRNA-1
chr2_1 CDS 23870995 23871237 23870995 ID=Msa0201920-mRNA-1:cds;Parent=Msa0201920-mRNA-1
chr2_1 CDS 23869905 23870432 23869905 ID=Msa0201920-mRNA-1:cds;Parent=Msa0201920-mRNA-1
chr2_1 three_prime_UTR 23869649 23869904 23869649 ID=Msa0201920-mRNA-1:three_prime_utr;Parent=Msa0201920-mRNA-1
Gene Sequence

>Msa0201920

ATGGGAACAAAAATTGAATATTCCATAAATCCTCTAGCATACTCAGTAGATTGCAACAACTTCAGTGTGGTTGGTGTTGATGCCTGGCAGCATTATCAGAACAAAGGATTGAAAGTGAGTAACAAACATTGCAATATTGGAGTTAATAACTTGCAAAATCCAATGGATAGGATACTTGACAGAAACAACATGGAATCCATTAGAAAGACAATGCAGATACAAGAGGATATCTTCAAAAACCAGGTAAAGGAATTACATAGAGTATACAATGTGCAAAAAATGCTAATGGATGAACACAAAAATGAAGGCAAGCAAAATAAATTTTGGACCCCTATGAATGGTAGCATAGGTATAAACCAACCTTATTTTGTTCAACAAAAACCAACACAAATTTCATTTAGCCATGTCCAAATTTTGAAAGAGGAATTATACATAAAGGAAAGAAGTGGAAGTTGTTCAGGAGAAATAATCAAGAAAAGACAAAAAGATTTTGATCTTGAAAAGCCAGCAGCAGCAAGTGATTGTGATGAAGATATGGAAGTGGATTTGACTTTATGTATAGGAAGCAATGAAAGTAAGAATAAGAAGAAAAAGTCTTATATGCTTCCAAATGGAATGAAAACTAAGGAGTTAAATTCTTATCTCTCTTTTCAATCAGATAGAATTGGAGATTATAGTGACCCCACTACACCAATGAGCAGCTCTAGTGTTACATTTGATCAAGATAAAAAGGGGCCACATTGGCTTTCTCAAGGTTTAAAGCTTAAATAG
Protein sequence

>Msa0201920

MGTKIEYSINPLAYSVDCNNFSVVGVDAWQHYQNKGLKVSNKHCNIGVNNLQNPMDRILDRNNMESIRKTMQIQEDIFKNQVKELHRVYNVQKMLMDEHKNEGKQNKFWTPMNGSIGINQPYFVQQKPTQISFSHVQILKEELYIKERSGSCSGEIIKKRQKDFDLEKPAAASDCDEDMEVDLTLCIGSNESKNKKKKSYMLPNGMKTKELNSYLSFQSDRIGDYSDPTTPMSSSSVTFDQDKKGPHWLSQGLKLK*