AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0282870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282870 MtrunA17_Chr2g0298071 95.331 257 7 3 1 256 1 253 0.0 499
Msa0282870 MtrunA17_Chr4g0072101 39.303 201 80 4 57 256 4 163 7.97e-32 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282870 AT5G57340.1 34.545 275 118 10 1 255 1 233 1.25e-21 90.5
Msa0282870 AT5G57340.2 33.455 275 121 10 1 255 19 251 2.48e-21 90.1

Find 64 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATAGTATTAAACTTTA+TGG 0.093335 2_3:+19315076 Msa0282870:five_prime_UTR
ATAATTCCTCTTTCAAAATT+TGG 0.105021 2_3:-19317760 None:intergenic
AAGGCAAGCAAAATAAATTT+TGG 0.180508 2_3:+19317657 Msa0282870:CDS
TACATTTGATCAAGATAAAA+AGG 0.235098 2_3:+19318063 Msa0282870:CDS
TTTACTTTATTCCAATAAAA+TGG 0.251455 2_3:+19318243 Msa0282870:three_prime_UTR
GGACCACTCAATTTATTATA+TGG 0.252972 2_3:+19318186 Msa0282870:three_prime_UTR
CTTGGCAGTTCATATTCTTT+TGG 0.254987 2_3:-19314914 None:intergenic
TGTTGTTGAGGTAATAATTT+AGG 0.265185 2_3:-19314828 None:intergenic
GAAAGAAGTGGAAGTTGTTC+AGG 0.278248 2_3:+19317791 Msa0282870:CDS
AGTAACAAACATTGCAATAT+TGG 0.285715 2_3:+19316908 Msa0282870:CDS
TATCTGCATTGTCTTTCTAA+TGG 0.285749 2_3:-19316987 None:intergenic
TGAGTATGCTAGAGGATTTA+TGG 0.299297 2_3:-19316813 None:intergenic
TCATATTCTTGAGATGCTTT+AGG 0.334440 2_3:-19314577 None:intergenic
ATACCTATGCTACCATTCAT+AGG 0.347754 2_3:-19317683 None:intergenic
GAAAGAGGAATTATACATAA+AGG 0.351691 2_3:+19317769 Msa0282870:CDS
GGTCCATGTTTAGTGTAAAT+AGG 0.365817 2_3:-19318168 None:intergenic
CACTTGTGTTATATTAGTAT+TGG 0.386558 2_3:+19315053 Msa0282870:five_prime_UTR
TCACAATCACTTGCTGCTGC+TGG 0.396014 2_3:-19317857 None:intergenic
TCTTCAAATTCTTCAGTGCT+TGG 0.405096 2_3:+19314961 Msa0282870:five_prime_UTR
GTGGATTTGACTTTATGTAT+AGG 0.411271 2_3:+19317893 Msa0282870:CDS
GAGCTGCTCATTGGTGTAGT+GGG 0.415795 2_3:-19318034 None:intergenic
GTGTGGTTGGTGTTGATGCC+TGG 0.418366 2_3:+19316855 Msa0282870:CDS
GGTAATAATTTAGGTTTGAC+AGG 0.427224 2_3:-19314819 None:intergenic
CTGTCAAGTATCCTATCCAT+TGG 0.443227 2_3:-19316950 None:intergenic
GAAGGAGGCAAGGCAAAAGC+TGG 0.453555 2_3:-19314788 None:intergenic
TTTGTTGAACAAAATAAGGT+TGG 0.453849 2_3:-19317709 None:intergenic
CTCAAGAATATGAATACAAT+GGG 0.453958 2_3:+19314587 Msa0282870:five_prime_UTR
TACCTATGCTACCATTCATA+GGG 0.460339 2_3:-19317682 None:intergenic
AAGTCTTATATGCTTCCAAA+TGG 0.472702 2_3:+19317944 Msa0282870:CDS
GCACTGAAGAATTTGAAGAT+AGG 0.487276 2_3:-19314957 None:intergenic
AGAGCTGCTCATTGGTGTAG+TGG 0.503631 2_3:-19318035 None:intergenic
TGAATACAATGGGATGTGAT+TGG 0.509342 2_3:+19314597 Msa0282870:five_prime_UTR
CCTCTTTCAAAATTTGGACA+TGG 0.511793 2_3:-19317754 None:intergenic
AAGTGATTGTGATGAAGATA+TGG 0.519183 2_3:+19317868 Msa0282870:CDS
TCCAAATGGAATGAAAACTA+AGG 0.522393 2_3:+19317958 Msa0282870:CDS
GTAACACTAGAGCTGCTCAT+TGG 0.525161 2_3:-19318043 None:intergenic
ATTCCTATTTACACTAAACA+TGG 0.531539 2_3:+19318165 Msa0282870:three_prime_UTR
TGGCTAAATGAAATTTGTGT+TGG 0.533437 2_3:-19317734 None:intergenic
TCTCAAGAATATGAATACAA+TGG 0.542189 2_3:+19314586 Msa0282870:five_prime_UTR
TAATAACTTGCAAAATCCAA+TGG 0.546440 2_3:+19316934 Msa0282870:CDS
ACTTGCAAAATCCAATGGAT+AGG 0.552356 2_3:+19316939 Msa0282870:CDS
GGGCCACATTGGCTTTCTCA+AGG 0.553022 2_3:+19318085 Msa0282870:CDS
CAATACTAATATAACACAAG+TGG 0.553056 2_3:-19315052 None:intergenic
GGCTGTGAAGGAAGGAGGCA+AGG 0.558019 2_3:-19314798 None:intergenic
GTTTGACAGGCTGTGAAGGA+AGG 0.558750 2_3:-19314806 None:intergenic
TTGTGATGAAGATATGGAAG+TGG 0.570304 2_3:+19317874 Msa0282870:CDS
TTTGTTCTGATAATGCTGCC+AGG 0.573507 2_3:-19316873 None:intergenic
CTGGAATAATGATGTTGTTG+AGG 0.583711 2_3:-19314840 None:intergenic
AAACATCAACAAAGCAACCT+AGG 0.585277 2_3:-19314887 None:intergenic
TGCAACAACTTCAGTGTGGT+TGG 0.585657 2_3:+19316842 Msa0282870:CDS
TTAGGTTTGACAGGCTGTGA+AGG 0.588272 2_3:-19314810 None:intergenic
AGCTGCTCATTGGTGTAGTG+GGG 0.600539 2_3:-19318033 None:intergenic
AAAGACAATGCAGATACAAG+AGG 0.602336 2_3:+19316994 Msa0282870:CDS
TTATACATAAAGGAAAGAAG+TGG 0.603251 2_3:+19317779 Msa0282870:CDS
CAATCTACTGAGTATGCTAG+AGG 0.610316 2_3:-19316821 None:intergenic
TGACAGGCTGTGAAGGAAGG+AGG 0.624890 2_3:-19314803 None:intergenic
GAACCATATAATAAATTGAG+TGG 0.626635 2_3:-19318189 None:intergenic
ATAGACAGCATGAAGACACT+TGG 0.628877 2_3:-19314932 None:intergenic
ACCCCTATGAATGGTAGCAT+AGG 0.633561 2_3:+19317680 Msa0282870:CDS
TGGCAGCATTATCAGAACAA+AGG 0.642212 2_3:+19316875 Msa0282870:CDS
AAACCTTGAGAAAGCCAATG+TGG 0.666219 2_3:-19318088 None:intergenic
ACCTATGCTACCATTCATAG+GGG 0.677238 2_3:-19317681 None:intergenic
GATACTTGACAGAAACAACA+TGG 0.687441 2_3:+19316961 Msa0282870:CDS
AGATTGCAACAACTTCAGTG+TGG 0.741905 2_3:+19316838 Msa0282870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAATAAAAGGA+CGG - chr2_3:19314993-19315012 None:intergenic 10.0%
!! TTGGAAAAAATAAAAATAAA+AGG - chr2_3:19314997-19315016 None:intergenic 10.0%
!! TTTAATAGTATTAAACTTTA+TGG + chr2_3:19315076-19315095 Msa0282870:five_prime_UTR 10.0%
!! TTGAAGAATAATAATAAAAT+AGG - chr2_3:19315963-19315982 None:intergenic 10.0%
!! AATTGAAAAAAATAAAATGA+TGG - chr2_3:19316385-19316404 None:intergenic 10.0%
!!! TTGTAAGTTTTTTATTTTTT+CGG + chr2_3:19316718-19316737 Msa0282870:intron 10.0%
!! ATTAGTATTTGAAAATTTAT+AGG + chr2_3:19317574-19317593 Msa0282870:intron 10.0%
!!! AAAAGAATTTTTTTCTTACA+TGG + chr2_3:19315375-19315394 Msa0282870:intron 15.0%
!! TTCTATTAATATTTAGTGTT+TGG + chr2_3:19316121-19316140 Msa0282870:intron 15.0%
!!! CAAATTTTAATTGTATTAGT+TGG + chr2_3:19317134-19317153 Msa0282870:intron 15.0%
!! CAAATGTAAAAAAAAATTGT+TGG + chr2_3:19317219-19317238 Msa0282870:intron 15.0%
!! TTTGTACAATTTATTCAATT+GGG - chr2_3:19317434-19317453 None:intergenic 15.0%
!!! TTTTGTACAATTTATTCAAT+TGG - chr2_3:19317435-19317454 None:intergenic 15.0%
!! ATTTGAAAATTTATAGGTAA+AGG + chr2_3:19317580-19317599 Msa0282870:intron 15.0%
!! TTTACTTTATTCCAATAAAA+TGG + chr2_3:19318243-19318262 Msa0282870:three_prime_UTR 15.0%
!!! ATATAAGTTATCCATTTTAT+TGG - chr2_3:19318257-19318276 None:intergenic 15.0%
!! ATAAAAAAAATTGTTCACCT+AGG + chr2_3:19314870-19314889 Msa0282870:five_prime_UTR 20.0%
!! AACTTTATGGATAAAAATGT+TGG + chr2_3:19315089-19315108 Msa0282870:five_prime_UTR 20.0%
!! AAGGATTATGAATTATGATT+AGG - chr2_3:19315222-19315241 None:intergenic 20.0%
!! ATACTCAAGAAAAGAAAAAA+AGG - chr2_3:19315241-19315260 None:intergenic 20.0%
!! TTAAGTGTATATATATGTGA+AGG + chr2_3:19315548-19315567 Msa0282870:intron 20.0%
!! TAAGTGTATATATATGTGAA+GGG + chr2_3:19315549-19315568 Msa0282870:intron 20.0%
!!! AAGATATTATTTTCAGATCA+TGG + chr2_3:19316054-19316073 Msa0282870:intron 20.0%
!! GTCAAAATATTCTTAGAATA+AGG + chr2_3:19316097-19316116 Msa0282870:intron 20.0%
!! TATGTTATTAAGTTAGTTAG+AGG - chr2_3:19316471-19316490 None:intergenic 20.0%
!! TAAACTAGATATTGACAATT+TGG - chr2_3:19316659-19316678 None:intergenic 20.0%
!!! GCTTCTTTAGTTTTAAATTA+AGG + chr2_3:19317156-19317175 Msa0282870:intron 20.0%
!!! TGCACAAGTATTAAAAATTT+TGG + chr2_3:19317287-19317306 Msa0282870:intron 20.0%
!!! GCACAAGTATTAAAAATTTT+GGG + chr2_3:19317288-19317307 Msa0282870:intron 20.0%
!! TACCCTTAAATTAAATGATT+TGG + chr2_3:19317347-19317366 Msa0282870:intron 20.0%
!! ACCCTTAAATTAAATGATTT+GGG + chr2_3:19317348-19317367 Msa0282870:intron 20.0%
!!! ACCCAAATCATTTAATTTAA+GGG - chr2_3:19317352-19317371 None:intergenic 20.0%
!!! AACCCAAATCATTTAATTTA+AGG - chr2_3:19317353-19317372 None:intergenic 20.0%
!! TTGTACAATTTATTCAATTG+GGG - chr2_3:19317433-19317452 None:intergenic 20.0%
!! ATAATTCCTCTTTCAAAATT+TGG - chr2_3:19317763-19317782 None:intergenic 20.0%
!! TACATTTGATCAAGATAAAA+AGG + chr2_3:19318063-19318082 Msa0282870:CDS 20.0%
!! ACATTTGATCAAGATAAAAA+GGG + chr2_3:19318064-19318083 Msa0282870:CDS 20.0%
!! ATAAAGTAAAAAACTCATCT+AGG - chr2_3:19318233-19318252 None:intergenic 20.0%
! TCTCAAGAATATGAATACAA+TGG + chr2_3:19314586-19314605 Msa0282870:five_prime_UTR 25.0%
! CTCAAGAATATGAATACAAT+GGG + chr2_3:19314587-19314606 Msa0282870:five_prime_UTR 25.0%
! TGTTGTTGAGGTAATAATTT+AGG - chr2_3:19314831-19314850 None:intergenic 25.0%
!! TTTATGTTTTCCTTGAATCA+TGG - chr2_3:19315020-19315039 None:intergenic 25.0%
! CAATACTAATATAACACAAG+TGG - chr2_3:19315055-19315074 None:intergenic 25.0%
! CACTTGTGTTATATTAGTAT+TGG + chr2_3:19315053-19315072 Msa0282870:five_prime_UTR 25.0%
! TACTAAACCAAAAATAGAAG+AGG - chr2_3:19315890-19315909 None:intergenic 25.0%
! TTGAATCTATGCATACAAAA+AGG + chr2_3:19316014-19316033 Msa0282870:intron 25.0%
!!! TAAGTATTGATTTGCATTTC+AGG + chr2_3:19316767-19316786 Msa0282870:intron 25.0%
! AGTAACAAACATTGCAATAT+TGG + chr2_3:19316908-19316927 Msa0282870:CDS 25.0%
!! TAATAACTTGCAAAATCCAA+TGG + chr2_3:19316934-19316953 Msa0282870:CDS 25.0%
! TTGCTTAACATATGTCTTTA+GGG + chr2_3:19317311-19317330 Msa0282870:intron 25.0%
!!! ATTAAATGATTTGGGTTGAA+TGG + chr2_3:19317356-19317375 Msa0282870:intron 25.0%
! GCATCAAATAAAAACATAGT+TGG - chr2_3:19317508-19317527 None:intergenic 25.0%
!! AAGGCAAGCAAAATAAATTT+TGG + chr2_3:19317657-19317676 Msa0282870:CDS 25.0%
! TTTGTTGAACAAAATAAGGT+TGG - chr2_3:19317712-19317731 None:intergenic 25.0%
!! GGTTTTTGTTGAACAAAATA+AGG - chr2_3:19317716-19317735 None:intergenic 25.0%
! GAAAGAGGAATTATACATAA+AGG + chr2_3:19317769-19317788 Msa0282870:CDS 25.0%
! TTATACATAAAGGAAAGAAG+TGG + chr2_3:19317779-19317798 Msa0282870:CDS 25.0%
!! TCTTTTCAATCAGATAGAAT+TGG + chr2_3:19317998-19318017 Msa0282870:CDS 25.0%
! CATTTGATCAAGATAAAAAG+GGG + chr2_3:19318065-19318084 Msa0282870:CDS 25.0%
! ATTCCTATTTACACTAAACA+TGG + chr2_3:19318165-19318184 Msa0282870:three_prime_UTR 25.0%
! GAACCATATAATAAATTGAG+TGG - chr2_3:19318192-19318211 None:intergenic 25.0%
TCATATTCTTGAGATGCTTT+AGG - chr2_3:19314580-19314599 None:intergenic 30.0%
! GGTAATAATTTAGGTTTGAC+AGG - chr2_3:19314822-19314841 None:intergenic 30.0%
!!! TTTTTTCCAACCATGATTCA+AGG + chr2_3:19315007-19315026 Msa0282870:five_prime_UTR 30.0%
!! ATTTTGCTACTTCTTACAAC+AGG + chr2_3:19315158-19315177 Msa0282870:five_prime_UTR 30.0%
TCTTCACTCAAATAACATGA+AGG + chr2_3:19315268-19315287 Msa0282870:intron 30.0%
!! TGCATAGTTTCTAGTAATAG+TGG + chr2_3:19315722-19315741 Msa0282870:intron 30.0%
TCATTCATCATCAAAGTTTC+TGG + chr2_3:19315808-19315827 Msa0282870:intron 30.0%
! ACTAGTTCCTCTTCTATTTT+TGG + chr2_3:19315880-19315899 Msa0282870:intron 30.0%
GAGTAAATTTGTCAAAACAG+AGG - chr2_3:19315919-19315938 None:intergenic 30.0%
GTTAGTACAGTTGCTATATA+TGG + chr2_3:19315990-19316009 Msa0282870:intron 30.0%
ATGGTTTGAATTTCTGTTGT+TGG + chr2_3:19316073-19316092 Msa0282870:intron 30.0%
TCTTAAGCACAAACAGAAAA+GGG + chr2_3:19316199-19316218 Msa0282870:intron 30.0%
TAGAGACTAACAAGTCTAAT+AGG - chr2_3:19316248-19316267 None:intergenic 30.0%
TGGAATATATCTACGTATGT+TGG - chr2_3:19316365-19316384 None:intergenic 30.0%
!! ATTGATTTGCATTTCAGGAA+TGG + chr2_3:19316772-19316791 Msa0282870:intron 30.0%
!! TTGATTTGCATTTCAGGAAT+GGG + chr2_3:19316773-19316792 Msa0282870:intron 30.0%
TATCTGCATTGTCTTTCTAA+TGG - chr2_3:19316990-19317009 None:intergenic 30.0%
TCCATTCAATTTGTACCATA+AGG + chr2_3:19317078-19317097 Msa0282870:intron 30.0%
ACCTTATGGTACAAATTGAA+TGG - chr2_3:19317082-19317101 None:intergenic 30.0%
! ATAGCATGACCAATTTTCAT+AGG + chr2_3:19317105-19317124 Msa0282870:intron 30.0%
TTGATCCATCCTATGAAAAT+TGG - chr2_3:19317117-19317136 None:intergenic 30.0%
CTCGTTAACTTATGTCTTAA+GGG - chr2_3:19317198-19317217 None:intergenic 30.0%
ACTCGTTAACTTATGTCTTA+AGG - chr2_3:19317199-19317218 None:intergenic 30.0%
GTTGCTTAACATATGTCTTT+AGG + chr2_3:19317310-19317329 Msa0282870:intron 30.0%
AATGAGAATAATGGCTTTCT+TGG - chr2_3:19317389-19317408 None:intergenic 30.0%
GTTTGGCATAATGAGAATAA+TGG - chr2_3:19317398-19317417 None:intergenic 30.0%
! CATAGTTGGTATATGACAAT+AGG - chr2_3:19317494-19317513 None:intergenic 30.0%
CAATGTGCAAAAAATGCTAA+TGG + chr2_3:19317619-19317638 Msa0282870:CDS 30.0%
ATGGATGAACACAAAAATGA+AGG + chr2_3:19317638-19317657 Msa0282870:CDS 30.0%
TGGCTAAATGAAATTTGTGT+TGG - chr2_3:19317737-19317756 None:intergenic 30.0%
AAGTGATTGTGATGAAGATA+TGG + chr2_3:19317868-19317887 Msa0282870:CDS 30.0%
! GTGGATTTGACTTTATGTAT+AGG + chr2_3:19317893-19317912 Msa0282870:CDS 30.0%
! AAGTCTTATATGCTTCCAAA+TGG + chr2_3:19317944-19317963 Msa0282870:CDS 30.0%
TCCAAATGGAATGAAAACTA+AGG + chr2_3:19317958-19317977 Msa0282870:CDS 30.0%
!! TCCTTAGTTTTCATTCCATT+TGG - chr2_3:19317962-19317981 None:intergenic 30.0%
GGACCACTCAATTTATTATA+TGG + chr2_3:19318186-19318205 Msa0282870:three_prime_UTR 30.0%
TGAATACAATGGGATGTGAT+TGG + chr2_3:19314597-19314616 Msa0282870:five_prime_UTR 35.0%
CTGGAATAATGATGTTGTTG+AGG - chr2_3:19314843-19314862 None:intergenic 35.0%
AAACATCAACAAAGCAACCT+AGG - chr2_3:19314890-19314909 None:intergenic 35.0%
! CTTGGCAGTTCATATTCTTT+TGG - chr2_3:19314917-19314936 None:intergenic 35.0%
GCACTGAAGAATTTGAAGAT+AGG - chr2_3:19314960-19314979 None:intergenic 35.0%
TCTTCAAATTCTTCAGTGCT+TGG + chr2_3:19314961-19314980 Msa0282870:five_prime_UTR 35.0%
! TGTTTTCCTTGAATCATGGT+TGG - chr2_3:19315016-19315035 None:intergenic 35.0%
TAAGCTTAACCTATGCAACA+CGG + chr2_3:19315413-19315432 Msa0282870:intron 35.0%
GATGAATGAACATTATGTGC+AGG - chr2_3:19315797-19315816 None:intergenic 35.0%
CTCTTAAGCACAAACAGAAA+AGG + chr2_3:19316198-19316217 Msa0282870:intron 35.0%
GATCACAAGACTTTAAGTGT+AGG - chr2_3:19316556-19316575 None:intergenic 35.0%
CTTCAATCAGATTGAAGTGT+AGG - chr2_3:19316580-19316599 None:intergenic 35.0%
AACATATGTCGATGTCATGT+CGG - chr2_3:19316622-19316641 None:intergenic 35.0%
TGAGTATGCTAGAGGATTTA+TGG - chr2_3:19316816-19316835 None:intergenic 35.0%
ACTTGCAAAATCCAATGGAT+AGG + chr2_3:19316939-19316958 Msa0282870:CDS 35.0%
GATACTTGACAGAAACAACA+TGG + chr2_3:19316961-19316980 Msa0282870:CDS 35.0%
AAAGACAATGCAGATACAAG+AGG + chr2_3:19316994-19317013 Msa0282870:CDS 35.0%
AGAGGATATCTTCAAAAACC+AGG + chr2_3:19317012-19317031 Msa0282870:CDS 35.0%
TACTACATTGACATGATACC+TGG - chr2_3:19317033-19317052 None:intergenic 35.0%
! CATGACCAATTTTCATAGGA+TGG + chr2_3:19317109-19317128 Msa0282870:intron 35.0%
TTATTCAATTGGGGACTTGA+AGG - chr2_3:19317424-19317443 None:intergenic 35.0%
! AAATTTTGGACCCCTATGAA+TGG + chr2_3:19317671-19317690 Msa0282870:CDS 35.0%
TACCTATGCTACCATTCATA+GGG - chr2_3:19317685-19317704 None:intergenic 35.0%
ATACCTATGCTACCATTCAT+AGG - chr2_3:19317686-19317705 None:intergenic 35.0%
CCTCTTTCAAAATTTGGACA+TGG - chr2_3:19317757-19317776 None:intergenic 35.0%
!! CCATGTCCAAATTTTGAAAG+AGG + chr2_3:19317754-19317773 Msa0282870:CDS 35.0%
TTGTGATGAAGATATGGAAG+TGG + chr2_3:19317874-19317893 Msa0282870:CDS 35.0%
GGTCCATGTTTAGTGTAAAT+AGG - chr2_3:19318171-19318190 None:intergenic 35.0%
ATAGACAGCATGAAGACACT+TGG - chr2_3:19314935-19314954 None:intergenic 40.0%
AACACGGACACTTCTCATAT+TGG + chr2_3:19315429-19315448 Msa0282870:intron 40.0%
ACACGGACACTTCTCATATT+GGG + chr2_3:19315430-19315449 Msa0282870:intron 40.0%
CACTTAAGCTCTATGAAGCA+TGG - chr2_3:19315535-19315554 None:intergenic 40.0%
GTGGATGAAGACTACTGTAA+TGG + chr2_3:19315741-19315760 Msa0282870:intron 40.0%
! TGTGTTTCTGTCACAAGACT+AGG - chr2_3:19315768-19315787 None:intergenic 40.0%
! ACGTCTTCTATCTGAAGTGT+CGG - chr2_3:19316310-19316329 None:intergenic 40.0%
CTTCAGATAGAAGACGTGTA+TGG + chr2_3:19316313-19316332 Msa0282870:intron 40.0%
GTATGGTGACTAACACGTAT+TGG + chr2_3:19316330-19316349 Msa0282870:intron 40.0%
! TGACTAACACGTATTGGTGT+TGG + chr2_3:19316336-19316355 Msa0282870:intron 40.0%
! AAGTTAGTTAGAGGAAGCAC+CGG - chr2_3:19316462-19316481 None:intergenic 40.0%
AGTGTAGGAGATTGAAGTGT+AGG - chr2_3:19316541-19316560 None:intergenic 40.0%
AATCTGATTGAAGTCGTGTC+TGG + chr2_3:19316587-19316606 Msa0282870:intron 40.0%
CAATCTACTGAGTATGCTAG+AGG - chr2_3:19316824-19316843 None:intergenic 40.0%
AGATTGCAACAACTTCAGTG+TGG + chr2_3:19316838-19316857 Msa0282870:CDS 40.0%
! TTTGTTCTGATAATGCTGCC+AGG - chr2_3:19316876-19316895 None:intergenic 40.0%
TGGCAGCATTATCAGAACAA+AGG + chr2_3:19316875-19316894 Msa0282870:CDS 40.0%
CTGTCAAGTATCCTATCCAT+TGG - chr2_3:19316953-19316972 None:intergenic 40.0%
ACCTATGCTACCATTCATAG+GGG - chr2_3:19317684-19317703 None:intergenic 40.0%
GAAAGAAGTGGAAGTTGTTC+AGG + chr2_3:19317791-19317810 Msa0282870:CDS 40.0%
AAGATAAAAAGGGGCCACAT+TGG + chr2_3:19318074-19318093 Msa0282870:CDS 40.0%
AAACCTTGAGAAAGCCAATG+TGG - chr2_3:19318091-19318110 None:intergenic 40.0%
TTTGTGTGAGAGAGAGAGAG+AGG - chr2_3:19314727-19314746 None:intergenic 45.0%
TTAGGTTTGACAGGCTGTGA+AGG - chr2_3:19314813-19314832 None:intergenic 45.0%
CACGGACACTTCTCATATTG+GGG + chr2_3:19315431-19315450 Msa0282870:intron 45.0%
GTCAGTGTATGTGTTGTGTC+TGG + chr2_3:19315494-19315513 Msa0282870:intron 45.0%
TCCAATATCCGTGTCTTTGC+CGG + chr2_3:19316440-19316459 Msa0282870:intron 45.0%
TGCAACAACTTCAGTGTGGT+TGG + chr2_3:19316842-19316861 Msa0282870:CDS 45.0%
GGTCATGCTATGCAACCTTA+TGG - chr2_3:19317096-19317115 None:intergenic 45.0%
ACCCCTATGAATGGTAGCAT+AGG + chr2_3:19317680-19317699 Msa0282870:CDS 45.0%
GTAACACTAGAGCTGCTCAT+TGG - chr2_3:19318046-19318065 None:intergenic 45.0%
!!! AATTTTTTTTATATAAAAAC+TGG - chr2_3:19314862-19314881 None:intergenic 5.0%
!!! TTATTTTTTTCAATTATTAT+TGG + chr2_3:19316390-19316409 Msa0282870:intron 5.0%
GTTTGACAGGCTGTGAAGGA+AGG - chr2_3:19314809-19314828 None:intergenic 50.0%
!! GAGAAGTGTCCGTGTTGCAT+AGG - chr2_3:19315425-19315444 None:intergenic 50.0%
! CTTCTCATATTGGGGTGTCC+TGG + chr2_3:19315439-19315458 Msa0282870:intron 50.0%
! ATATTGGGGTGTCCTGGTGT+CGG + chr2_3:19315445-19315464 Msa0282870:intron 50.0%
ATACACTGACATGCCGACAC+CGG - chr2_3:19315484-19315503 None:intergenic 50.0%
ACCGGCAAAGACACGGATAT+TGG - chr2_3:19316444-19316463 None:intergenic 50.0%
!! TGGGGACTTGAAGGAGTGTT+TGG - chr2_3:19317415-19317434 None:intergenic 50.0%
! TCACAATCACTTGCTGCTGC+TGG - chr2_3:19317860-19317879 None:intergenic 50.0%
AGCTGCTCATTGGTGTAGTG+GGG - chr2_3:19318036-19318055 None:intergenic 50.0%
GAGCTGCTCATTGGTGTAGT+GGG - chr2_3:19318037-19318056 None:intergenic 50.0%
AGAGCTGCTCATTGGTGTAG+TGG - chr2_3:19318038-19318057 None:intergenic 50.0%
GAAGGAGGCAAGGCAAAAGC+TGG - chr2_3:19314791-19314810 None:intergenic 55.0%
TGACAGGCTGTGAAGGAAGG+AGG - chr2_3:19314806-19314825 None:intergenic 55.0%
TGTCGGATACGTGTCGTGTC+CGG + chr2_3:19315462-19315481 Msa0282870:intron 55.0%
ATACGTGTCGTGTCCGGTGT+CGG + chr2_3:19315468-19315487 Msa0282870:intron 55.0%
!! AGGAAGCACCGGCAAAGACA+CGG - chr2_3:19316451-19316470 None:intergenic 55.0%
!! GTGTGGTTGGTGTTGATGCC+TGG + chr2_3:19316855-19316874 Msa0282870:CDS 55.0%
!! GGGCCACATTGGCTTTCTCA+AGG + chr2_3:19318085-19318104 Msa0282870:CDS 55.0%
GGCTGTGAAGGAAGGAGGCA+AGG - chr2_3:19314801-19314820 None:intergenic 60.0%
CACGACACGTATCCGACACC+AGG - chr2_3:19315460-19315479 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 19314570 19318379 19314570 ID=Msa0282870;Name=Msa0282870
chr2_3 mRNA 19314570 19318379 19314570 ID=Msa0282870-mRNA-1;Parent=Msa0282870;Name=Msa0282870-mRNA-1;_AED=0.03;_eAED=0.03;_QI=612|1|1|1|0.5|0.66|3|256|256
chr2_3 exon 19314570 19315179 19314570 ID=Msa0282870-mRNA-1:exon:7801;Parent=Msa0282870-mRNA-1
chr2_3 exon 19316789 19317033 19316789 ID=Msa0282870-mRNA-1:exon:7802;Parent=Msa0282870-mRNA-1
chr2_3 exon 19317596 19318379 19317596 ID=Msa0282870-mRNA-1:exon:7803;Parent=Msa0282870-mRNA-1
chr2_3 five_prime_UTR 19314570 19315179 19314570 ID=Msa0282870-mRNA-1:five_prime_utr;Parent=Msa0282870-mRNA-1
chr2_3 five_prime_UTR 19316789 19316790 19316789 ID=Msa0282870-mRNA-1:five_prime_utr;Parent=Msa0282870-mRNA-1
chr2_3 CDS 19316791 19317033 19316791 ID=Msa0282870-mRNA-1:cds;Parent=Msa0282870-mRNA-1
chr2_3 CDS 19317596 19318123 19317596 ID=Msa0282870-mRNA-1:cds;Parent=Msa0282870-mRNA-1
chr2_3 three_prime_UTR 19318124 19318379 19318124 ID=Msa0282870-mRNA-1:three_prime_utr;Parent=Msa0282870-mRNA-1
Gene Sequence

>Msa0282870

ATGGGAACAAAAATTGAATATTCCATAAATCCTCTAGCATACTCAGTAGATTGCAACAACTTCAGTGTGGTTGGTGTTGATGCCTGGCAGCATTATCAGAACAAAGGATTGAAAGTGAGTAACAAACATTGCAATATTGGAGTTAATAACTTGCAAAATCCAATGGATAGGATACTTGACAGAAACAACATGGAATCCATTAGAAAGACAATGCAGATACAAGAGGATATCTTCAAAAACCAGGTAAAGGAATTACATAGAGTATACAATGTGCAAAAAATGCTAATGGATGAACACAAAAATGAAGGCAAGCAAAATAAATTTTGGACCCCTATGAATGGTAGCATAGGTATAAACCAACCTTATTTTGTTCAACAAAAACCAACACAAATTTCATTTAGCCATGTCCAAATTTTGAAAGAGGAATTATACATAAAGGAAAGAAGTGGAAGTTGTTCAGGAGAAATAATCAAGAAAAGACAAAAAGATTTTGATCTTGAAAAGCCAGCAGCAGCAAGTGATTGTGATGAAGATATGGAAGTGGATTTGACTTTATGTATAGGAAGCAATGAAAGTAAGAATAAGAAGAAAAAGTCTTATATGCTTCCAAATGGAATGAAAACTAAGGAGTTAAATTCTTATCTCTCTTTTCAATCAGATAGAATTGGAGATTATAGTGACCCCACTACACCAATGAGCAGCTCTAGTGTTACATTTGATCAAGATAAAAAGGGGCCACATTGGCTTTCTCAAGGTTTAAAGCTTAAATAG
Protein sequence

>Msa0282870

MGTKIEYSINPLAYSVDCNNFSVVGVDAWQHYQNKGLKVSNKHCNIGVNNLQNPMDRILDRNNMESIRKTMQIQEDIFKNQVKELHRVYNVQKMLMDEHKNEGKQNKFWTPMNGSIGINQPYFVQQKPTQISFSHVQILKEELYIKERSGSCSGEIIKKRQKDFDLEKPAAASDCDEDMEVDLTLCIGSNESKNKKKKSYMLPNGMKTKELNSYLSFQSDRIGDYSDPTTPMSSSSVTFDQDKKGPHWLSQGLKLK*