AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172400 MtrunA17_Chr1g0190641 97.468 79 2 0 1 79 8 86 8.52e-55 170
Msa0172400 MtrunA17_Chr1g0190711 62.264 159 6 3 81 185 95 253 4.81e-54 172
Msa0172400 MtrunA17_Chr1g0187921 50.318 157 25 2 81 185 81 236 2.52e-42 141
Msa0172400 MtrunA17_Chr1g0184791 50.943 159 24 2 81 185 87 245 9.96e-42 140
Msa0172400 MtrunA17_Chr7g0260641 45.860 157 30 2 81 185 97 250 2.21e-33 119
Msa0172400 MtrunA17_Chr7g0274181 48.819 127 49 4 18 144 70 180 1.22e-32 115
Msa0172400 MtrunA17_Chr1g0190661 56.383 94 37 2 2 91 10 103 1.77e-32 114
Msa0172400 MtrunA17_Chr1g0190651 60.000 85 30 2 2 82 10 94 1.94e-31 111
Msa0172400 MtrunA17_Chr5g0428141 43.590 156 35 2 81 185 194 347 5.41e-31 115
Msa0172400 MtrunA17_Chr5g0411501 41.520 171 44 3 69 185 167 335 6.79e-31 114
Msa0172400 MtrunA17_Chr1g0190671 54.255 94 39 2 2 91 10 103 1.13e-30 109
Msa0172400 MtrunA17_Chr6g0486121 42.138 159 35 3 81 185 99 254 1.26e-29 109
Msa0172400 MtrunA17_Chr8g0366881 42.138 159 35 3 81 185 193 348 3.16e-28 107
Msa0172400 MtrunA17_Chr2g0331411 40.994 161 35 4 81 185 170 326 3.41e-27 104
Msa0172400 MtrunA17_Chr8g0390281 39.375 160 38 3 81 185 138 293 8.58e-25 97.8
Msa0172400 MtrunA17_Chr7g0260621 41.353 133 28 2 81 174 81 202 8.54e-24 93.2
Msa0172400 MtrunA17_Chr6g0484021 50.000 86 36 2 1 79 30 115 6.60e-23 95.1
Msa0172400 MtrunA17_Chr1g0184801 40.602 133 28 2 81 174 82 202 5.24e-22 88.6
Msa0172400 MtrunA17_Chr1g0166011 39.098 133 28 3 81 174 65 183 2.10e-21 86.7
Msa0172400 MtrunA17_Chr1g0187961 38.636 132 28 3 81 173 61 178 2.48e-20 83.6
Msa0172400 MtrunA17_Chr6g0483971 48.148 81 39 2 4 83 13 91 2.03e-19 83.6
Msa0172400 MtrunA17_Chr6g0484031 46.250 80 38 2 1 79 10 85 1.40e-16 76.6
Msa0172400 MtrunA17_Chr4g0004181 36.957 138 31 5 80 177 51 172 1.45e-14 68.2
Msa0172400 MtrunA17_Chr8g0340731 42.683 82 35 1 84 153 108 189 1.69e-14 69.7
Msa0172400 MtrunA17_Chr6g0484041 41.860 86 41 3 1 79 22 105 4.50e-14 69.7
Msa0172400 MtrunA17_Chr2g0330951 41.860 86 35 2 84 154 97 182 2.99e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172400 AT4G14550.1 50.955 157 23 2 81 185 74 228 3.33e-41 139
Msa0172400 AT3G23050.1 38.696 230 57 5 15 185 39 243 7.96e-36 125
Msa0172400 AT3G04730.1 45.562 169 28 3 81 185 68 236 3.85e-35 123
Msa0172400 AT3G04720.1 47.407 135 55 4 15 146 74 195 2.94e-34 120
Msa0172400 AT4G14550.2 47.945 146 22 2 81 174 74 217 1.20e-33 119
Msa0172400 AT1G04250.1 44.654 159 25 3 81 185 80 229 1.27e-33 119
Msa0172400 AT4G14550.4 68.919 74 21 1 81 154 74 145 1.51e-28 105
Msa0172400 AT4G14550.3 52.830 106 33 2 81 171 74 177 3.96e-28 104
Msa0172400 AT1G04240.1 44.776 134 27 3 80 174 61 186 1.18e-26 100
Msa0172400 AT2G22670.3 40.506 158 38 3 81 184 163 318 1.85e-26 103
Msa0172400 AT2G22670.1 40.506 158 38 3 81 184 163 318 1.85e-26 103
Msa0172400 AT2G22670.4 40.506 158 38 3 81 184 180 335 2.16e-26 103
Msa0172400 AT1G04240.2 44.776 134 27 3 80 174 161 286 3.50e-26 101
Msa0172400 AT5G43700.1 44.361 133 25 3 81 174 60 182 6.84e-26 98.6
Msa0172400 AT3G23050.3 41.379 145 54 3 15 153 39 158 2.24e-24 94.0
Msa0172400 AT3G23050.2 37.079 178 66 5 15 171 39 191 1.99e-23 92.8
Msa0172400 AT2G22670.5 39.241 158 38 4 81 184 163 316 2.36e-23 94.7
Msa0172400 AT2G22670.2 39.241 158 38 4 81 184 163 316 2.36e-23 94.7
Msa0172400 AT4G29080.1 35.106 188 60 5 59 185 119 305 9.80e-23 92.8
Msa0172400 AT5G65670.2 58.108 74 26 2 81 153 181 250 1.42e-18 82.0
Msa0172400 AT5G65670.1 58.108 74 26 2 81 153 181 250 1.48e-18 82.0
Msa0172400 AT3G23030.1 38.636 132 23 2 81 173 58 170 4.13e-18 77.8
Msa0172400 AT3G23030.2 38.636 132 23 2 81 173 145 257 1.05e-17 78.6
Msa0172400 AT3G15540.1 37.857 140 37 4 81 180 68 197 9.58e-17 74.7
Msa0172400 AT1G15580.1 35.385 130 33 3 75 168 45 159 2.20e-14 67.8
Msa0172400 AT3G16500.1 40.449 89 41 1 78 154 98 186 1.08e-13 67.8
Msa0172400 AT3G16500.2 40.449 89 41 1 78 154 98 186 1.08e-13 67.8
Msa0172400 AT5G25890.1 33.333 132 51 2 78 176 43 170 6.59e-13 64.3
Msa0172400 AT4G14560.1 37.984 129 24 2 81 170 53 164 1.74e-12 62.8
Msa0172400 AT4G28640.1 29.747 158 44 4 82 174 87 242 1.33e-11 61.6
Msa0172400 AT4G28640.3 29.487 156 43 4 82 172 87 240 2.02e-11 61.2
Msa0172400 AT4G28640.2 29.221 154 46 3 82 172 87 240 3.79e-11 60.8

Find 68 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAACTCTTGTACAATTTA+AGG 0.136546 1_4:+57455658 None:intergenic
ACTGCCTTCTGTGGACCTGC+TGG 0.294447 1_4:-57457633 Msa0172400:CDS
TTGTCATGGCGTAGCAAATA+TGG 0.300884 1_4:-57457660 Msa0172400:CDS
CTCGAGCCAACTTGCTTCCT+TGG 0.317287 1_4:+57451779 None:intergenic
AAATTAGGTGGAAATTCATT+AGG 0.319576 1_4:-57451642 Msa0172400:three_prime_UTR
ACTGTTCTGTGTTCTTGTTT+TGG 0.321644 1_4:-57457799 Msa0172400:CDS
ATGTTTCCAGTTATCATTGC+TGG 0.325654 1_4:-57451508 Msa0172400:three_prime_UTR
ACAACCCTGAGCAACATAAC+TGG 0.370802 1_4:-57457734 Msa0172400:CDS
TTCTTGTGGCCGTTGCTTGA+GGG 0.373798 1_4:-57457595 Msa0172400:intron
TTCTTTCTAAAGGATCTAAC+TGG 0.382249 1_4:+57455801 None:intergenic
TTATATATGAATGAGAAATT+AGG 0.392785 1_4:-57451657 Msa0172400:three_prime_UTR
ATTTACTATGTTCTTTCTAA+AGG 0.395708 1_4:+57455791 None:intergenic
TCGAGCCAACTTGCTTCCTT+GGG 0.403910 1_4:+57451780 None:intergenic
ACAAATCCAGCAATGATAAC+TGG 0.406835 1_4:+57451502 None:intergenic
AGGTCCACCTTACGTAGATA+TGG 0.411796 1_4:+57455678 None:intergenic
ATGAAAGGTTCCGAGGCAAT+TGG 0.416704 1_4:-57452004 Msa0172400:CDS
CAATAAATATGATACGTATA+AGG 0.420782 1_4:+57451423 None:intergenic
CTTCTTGTGGCCGTTGCTTG+AGG 0.421339 1_4:-57457596 Msa0172400:CDS
CTGCCTTCTGTGGACCTGCT+GGG 0.422750 1_4:-57457632 Msa0172400:CDS
AAGTCCCAGTTATGTTGCTC+AGG 0.425651 1_4:+57457729 None:intergenic
AATTAGGTGGAAATTCATTA+GGG 0.434858 1_4:-57451641 Msa0172400:three_prime_UTR
AGTAAGGTCGAGGCTAATAT+AGG 0.437617 1_4:-57451758 Msa0172400:three_prime_UTR
TGTGGACCTGCTGGGCCTCA+AGG 0.438565 1_4:-57457624 Msa0172400:CDS
GCACATACTCTGTCACGTAT+TGG 0.454043 1_4:+57451457 None:intergenic
AGCACAAGTGGTGGTGGGAA+TGG 0.463834 1_4:-57455732 Msa0172400:CDS
GCTTGGCCTTGAGGCCCAGC+AGG 0.467802 1_4:+57457618 None:intergenic
CCAGCGCATCTGACAGCTCT+TGG 0.472063 1_4:+57455637 None:intergenic
TGAAAGGTTCCGAGGCAATT+GGG 0.476415 1_4:-57452003 Msa0172400:CDS
AATTTGTAGGGCACAAGTTG+TGG 0.477874 1_4:-57455832 Msa0172400:intron
CCAAGAGCTGTCAGATGCGC+TGG 0.479602 1_4:-57455637 Msa0172400:CDS
GATAAGAGCACAAGTGGTGG+TGG 0.485348 1_4:-57455738 Msa0172400:CDS
GAAGCAAGTTGGCTCGAGTA+AGG 0.490808 1_4:-57451774 Msa0172400:three_prime_UTR
AGCAACGGCCACAAGAAGCT+TGG 0.504478 1_4:+57457601 None:intergenic
TGCTACGCCATGACAAAGGC+TGG 0.514008 1_4:+57457667 None:intergenic
GGTTCCGAGGCAATTGGGCT+AGG 0.522558 1_4:-57451998 Msa0172400:intron
TTTACCTAGCCCAATTGCCT+CGG 0.542690 1_4:+57451994 None:intergenic
GCAGATTTATCAACCAGAAG+TGG 0.545785 1_4:+57451805 None:intergenic
TGGGAATGGAGCATTTGTTA+AGG 0.549372 1_4:-57455718 Msa0172400:CDS
ATTTGTAGGGCACAAGTTGT+GGG 0.551340 1_4:-57455831 Msa0172400:intron
CCTCAAGGCCAAGCTTCTTG+TGG 0.552466 1_4:-57457609 Msa0172400:CDS
CAACCCTGAGCAACATAACT+GGG 0.553138 1_4:-57457733 Msa0172400:CDS
AGGCCCAGCAGGTCCACAGA+AGG 0.557934 1_4:+57457629 None:intergenic
TATATGAATGAGAAATTAGG+TGG 0.560071 1_4:-57451654 Msa0172400:three_prime_UTR
ATGCAGACCAGCCTTTGTCA+TGG 0.566521 1_4:-57457674 Msa0172400:CDS
TTTGTTAAGGTGAGCATGGA+TGG 0.571730 1_4:-57455705 Msa0172400:CDS
CATGGCGTAGCAAATATGGC+TGG 0.575280 1_4:-57457656 Msa0172400:CDS
TATGGCTGGACTGCCTTCTG+TGG 0.577035 1_4:-57457642 Msa0172400:CDS
CCACAAGAAGCTTGGCCTTG+AGG 0.579744 1_4:+57457609 None:intergenic
GAAGCAGATAAGAGCACAAG+TGG 0.581742 1_4:-57455744 Msa0172400:CDS
AGTCCCAGTTATGTTGCTCA+GGG 0.592637 1_4:+57457730 None:intergenic
AAATATGATACGTATAAGGT+AGG 0.598381 1_4:+57451427 None:intergenic
GTAGGGCACAAGTTGTGGGA+TGG 0.599917 1_4:-57455827 Msa0172400:intron
TGCATCCCAAGGAAGCAAGT+TGG 0.600502 1_4:-57451785 Msa0172400:three_prime_UTR
AGCATTTGTTAAGGTGAGCA+TGG 0.602441 1_4:-57455709 Msa0172400:CDS
AGTTGGCTCGAGTAAGGTCG+AGG 0.607074 1_4:-57451768 Msa0172400:three_prime_UTR
AGGGTTGTATAAATTAAATG+TGG 0.608521 1_4:+57457749 None:intergenic
TGGTGCACCATATCTACGTA+AGG 0.613344 1_4:-57455685 Msa0172400:CDS
TTTCTAAAGGATCTAACTGG+TGG 0.617257 1_4:+57455804 None:intergenic
GCAGATAAGAGCACAAGTGG+TGG 0.620410 1_4:-57455741 Msa0172400:CDS
AAGCGTCTTCGCATAATGAA+AGG 0.624465 1_4:-57452019 Msa0172400:CDS
TATTTGCTACGCCATGACAA+AGG 0.626209 1_4:+57457663 None:intergenic
ATAAGAGCACAAGTGGTGGT+GGG 0.631680 1_4:-57455737 Msa0172400:CDS
GTTGATAAATCTGCATCCCA+AGG 0.636946 1_4:-57451796 Msa0172400:three_prime_UTR
CCAAGCATACCCTCAAGCAA+CGG 0.639130 1_4:+57457586 None:intergenic
ATTATAAGTCAAGCACACGC+TGG 0.650658 1_4:+57451538 None:intergenic
TCGCATAATGAAAGGTTCCG+AGG 0.668062 1_4:-57452011 Msa0172400:CDS
TGCACCATATCTACGTAAGG+TGG 0.669757 1_4:-57455682 Msa0172400:CDS
ATTAAATGTGGACTGCACAG+TGG 0.717942 1_4:+57457761 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATTTATATATTGGA+TGG - chr1_4:57451694-57451713 Msa0172400:three_prime_UTR 10.0%
!!! CTTATAAAAATGTTTTAATT+AGG - chr1_4:57451857-57451876 Msa0172400:CDS 10.0%
!!! AATTTTATTCAAAAAATTTG+AGG + chr1_4:57452502-57452521 None:intergenic 10.0%
!!! AATTTTTAAAAGAATAAACA+TGG - chr1_4:57452655-57452674 Msa0172400:intron 10.0%
!!! AATAATAGTATTATTTTAGT+TGG - chr1_4:57452825-57452844 Msa0172400:intron 10.0%
!!! AAATAAATAGATTTTATAGT+GGG + chr1_4:57453852-57453871 None:intergenic 10.0%
!! TATAAAATCTATTTATTTGT+GGG - chr1_4:57453854-57453873 Msa0172400:intron 10.0%
!! AAGAAAAAAATTATAATATG+GGG + chr1_4:57453890-57453909 None:intergenic 10.0%
!!! AAAATGTTTATAGAAATTTT+GGG - chr1_4:57456681-57456700 Msa0172400:intron 10.0%
!! ATATTATTGTTTAACAAAAC+AGG + chr1_4:57452254-57452273 None:intergenic 15.0%
!!! AAGTTTAATGTTAGTTTTTA+TGG + chr1_4:57452311-57452330 None:intergenic 15.0%
!! ATTAAAATTCATGTCAATAT+AGG + chr1_4:57452528-57452547 None:intergenic 15.0%
!!! TGTAATAAAATTGATTTTGA+AGG + chr1_4:57452596-57452615 None:intergenic 15.0%
!!! AAAAAAAATAAAGTATTGGA+TGG - chr1_4:57452774-57452793 Msa0172400:intron 15.0%
!! AATTAACAATATTATATGAG+TGG + chr1_4:57452934-57452953 None:intergenic 15.0%
!! TTAATGAATATTGAATTTGT+AGG - chr1_4:57453345-57453364 Msa0172400:intron 15.0%
!! TAATGAATATTGAATTTGTA+GGG - chr1_4:57453346-57453365 Msa0172400:intron 15.0%
!!! AAATAATTCTTTTCAATTAG+GGG + chr1_4:57453820-57453839 None:intergenic 15.0%
!!! CAAATAATTCTTTTCAATTA+GGG + chr1_4:57453821-57453840 None:intergenic 15.0%
!! CTAATTGAAAAGAATTATTT+GGG - chr1_4:57453820-57453839 Msa0172400:intron 15.0%
!! TAATTGAAAAGAATTATTTG+GGG - chr1_4:57453821-57453840 Msa0172400:intron 15.0%
!!! CAAATAAATAGATTTTATAG+TGG + chr1_4:57453853-57453872 None:intergenic 15.0%
!! CTATAAAATCTATTTATTTG+TGG - chr1_4:57453853-57453872 Msa0172400:intron 15.0%
!!! GAAAATGTTTATAGAAATTT+TGG - chr1_4:57456680-57456699 Msa0172400:intron 15.0%
!!! AAATGTTTATAGAAATTTTG+GGG - chr1_4:57456682-57456701 Msa0172400:intron 15.0%
!!! ACGATGTAATAAATTTTTAT+TGG + chr1_4:57456968-57456987 None:intergenic 15.0%
!! TCAAATGTATTTAACAAATT+AGG - chr1_4:57457245-57457264 Msa0172400:intron 15.0%
!! TTATATATGAATGAGAAATT+AGG - chr1_4:57457533-57457552 Msa0172400:intron 15.0%
!! TTGGACAAAATAAAAATAAT+GGG + chr1_4:57457717-57457736 None:intergenic 15.0%
!! ATTGGACAAAATAAAAATAA+TGG + chr1_4:57457718-57457737 None:intergenic 15.0%
!!! ATGACTTAAATACTTTTAGA+TGG + chr1_4:57451670-57451689 None:intergenic 20.0%
!!! AATGTTTTAATTAGGTTAGA+AGG - chr1_4:57451865-57451884 Msa0172400:CDS 20.0%
!!! TTTTTTGAACATGACAATTT+TGG - chr1_4:57451975-57451994 Msa0172400:intron 20.0%
!! ATTTGTTTGTGAAAAACTAT+TGG - chr1_4:57452031-57452050 Msa0172400:CDS 20.0%
!!! TTTATTTATCCTTTTGTTTG+GGG + chr1_4:57452958-57452977 None:intergenic 20.0%
!!! CTTTATTTATCCTTTTGTTT+GGG + chr1_4:57452959-57452978 None:intergenic 20.0%
!!! ACTTTATTTATCCTTTTGTT+TGG + chr1_4:57452960-57452979 None:intergenic 20.0%
!! AAGGATAAATAAAGTTGAAA+AGG - chr1_4:57452965-57452984 Msa0172400:intron 20.0%
!! GTAATTTATGCATGAATTAT+TGG + chr1_4:57453052-57453071 None:intergenic 20.0%
!! ATTTGTGTTTAATTGGAAAT+TGG - chr1_4:57453093-57453112 Msa0172400:intron 20.0%
!! ATCTGTTTAATAAGATTAGT+TGG - chr1_4:57453297-57453316 Msa0172400:intron 20.0%
!! ATTTACTATGTTCTTTCTAA+AGG + chr1_4:57453402-57453421 None:intergenic 20.0%
!!! AATAATTCTTTTCAATTAGG+GGG + chr1_4:57453819-57453838 None:intergenic 20.0%
!!! CCAAATAATTCTTTTCAATT+AGG + chr1_4:57453822-57453841 None:intergenic 20.0%
!! CCTAATTGAAAAGAATTATT+TGG - chr1_4:57453819-57453838 Msa0172400:intron 20.0%
!! TTATGCAAAACAACTTATTA+CGG - chr1_4:57453962-57453981 Msa0172400:intron 20.0%
!! TAAAAATCCACAAGATTAAA+AGG + chr1_4:57456755-57456774 None:intergenic 20.0%
!! AAATTAAACAATGTAATGTG+AGG + chr1_4:57456864-57456883 None:intergenic 20.0%
!! TGGACAAAATAAAAATAATG+GGG + chr1_4:57457716-57457735 None:intergenic 20.0%
!! CAATAAATATGATACGTATA+AGG + chr1_4:57457770-57457789 None:intergenic 20.0%
! AGGGTTGTATAAATTAAATG+TGG + chr1_4:57451444-57451463 None:intergenic 25.0%
! AAAATAAAGTTTACACGAGT+TGG - chr1_4:57451729-57451748 Msa0172400:three_prime_UTR 25.0%
!! GTTTCTTGTTCCATAATTTT+TGG - chr1_4:57451923-57451942 Msa0172400:intron 25.0%
! ACGAAATCTTATAAGCATAT+TGG + chr1_4:57452288-57452307 None:intergenic 25.0%
!! ATAATCTGGATGACATTTTT+AGG + chr1_4:57452727-57452746 None:intergenic 25.0%
!! AATTTTTCTTGCACATAATC+TGG + chr1_4:57452741-57452760 None:intergenic 25.0%
!!! AAAAATAAAGTATTGGATGG+TGG - chr1_4:57452777-57452796 Msa0172400:intron 25.0%
!!! TTTTAGTTGGATTTTTTCTG+CGG - chr1_4:57452838-57452857 Msa0172400:intron 25.0%
!!! TTATTTATCCTTTTGTTTGG+GGG + chr1_4:57452957-57452976 None:intergenic 25.0%
! AGACTATATAACAATGTGAT+AGG + chr1_4:57453025-57453044 None:intergenic 25.0%
! CCAATTAAACACAAATTATC+TGG + chr1_4:57453089-57453108 None:intergenic 25.0%
!! CCAGATAATTTGTGTTTAAT+TGG - chr1_4:57453086-57453105 Msa0172400:intron 25.0%
! TGAACATCAATGATCTTAAT+CGG + chr1_4:57453173-57453192 None:intergenic 25.0%
!! AAATTGATTGACCTTTTGAA+CGG - chr1_4:57453647-57453666 Msa0172400:intron 25.0%
!!! TTATAATATGGGGCTTTATT+AGG + chr1_4:57453880-57453899 None:intergenic 25.0%
!! AAATTAGGTTTAGAACCATA+TGG - chr1_4:57457260-57457279 Msa0172400:intron 25.0%
! ATAAGATAAGATGTACCATA+TGG + chr1_4:57457278-57457297 None:intergenic 25.0%
! TATATGAATGAGAAATTAGG+TGG - chr1_4:57457536-57457555 Msa0172400:intron 25.0%
! AAATTAGGTGGAAATTCATT+AGG - chr1_4:57457548-57457567 Msa0172400:intron 25.0%
! AATTAGGTGGAAATTCATTA+GGG - chr1_4:57457549-57457568 Msa0172400:intron 25.0%
!!! ACCCAGTTTTTGTTATTATA+AGG - chr1_4:57457602-57457621 Msa0172400:CDS 25.0%
! CCCTTATAATAACAAAAACT+GGG + chr1_4:57457606-57457625 None:intergenic 25.0%
!!! CCCAGTTTTTGTTATTATAA+GGG - chr1_4:57457603-57457622 Msa0172400:CDS 25.0%
!!! CCAGTTTTTGTTATTATAAG+GGG - chr1_4:57457604-57457623 Msa0172400:CDS 25.0%
!! GACAAAATAAAAATAATGGG+GGG + chr1_4:57457714-57457733 None:intergenic 25.0%
! GGACAAAATAAAAATAATGG+GGG + chr1_4:57457715-57457734 None:intergenic 25.0%
! AAATATGATACGTATAAGGT+AGG + chr1_4:57457766-57457785 None:intergenic 25.0%
GTTTGTGATTAAAGATCACA+CGG - chr1_4:57451890-57451909 Msa0172400:intron 30.0%
GCAAATTCTGCCAAAAATTA+TGG + chr1_4:57451936-57451955 None:intergenic 30.0%
! TTTGAACATGACAATTTTGG+TGG - chr1_4:57451978-57451997 Msa0172400:intron 30.0%
! ATTGGCAATTTGAGTTTGAT+TGG - chr1_4:57452049-57452068 Msa0172400:intron 30.0%
AAAGAAAAAGACAAGACACT+TGG - chr1_4:57452888-57452907 Msa0172400:intron 30.0%
! TTCTTTCTAAAGGATCTAAC+TGG + chr1_4:57453392-57453411 None:intergenic 30.0%
GGTAACTCTTGTACAATTTA+AGG + chr1_4:57453535-57453554 None:intergenic 30.0%
!! TTATGTGGGCTTTGAAATTT+GGG + chr1_4:57453765-57453784 None:intergenic 30.0%
!! TTTATGTGGGCTTTGAAATT+TGG + chr1_4:57453766-57453785 None:intergenic 30.0%
!! TTTAATTTAGTGGGTTTGGT+GGG + chr1_4:57453795-57453814 None:intergenic 30.0%
!!! AATAGATTTTATAGTGGGCT+TGG + chr1_4:57453847-57453866 None:intergenic 30.0%
AACAGCTTATTTGTGCAAAA+CGG + chr1_4:57454162-57454181 None:intergenic 30.0%
!!! TTATAGAAATTTTGGGGCTA+AGG - chr1_4:57456688-57456707 Msa0172400:intron 30.0%
!!! TATAGAAATTTTGGGGCTAA+GGG - chr1_4:57456689-57456708 Msa0172400:intron 30.0%
AATGTGAGGTGAAAAAAAGA+GGG + chr1_4:57456850-57456869 None:intergenic 30.0%
TAATGTGAGGTGAAAAAAAG+AGG + chr1_4:57456851-57456870 None:intergenic 30.0%
! TAATCTGATGATGAATGGAT+TGG + chr1_4:57456929-57456948 None:intergenic 30.0%
AAAAATTGCGTAGATTGGAT+TGG + chr1_4:57457010-57457029 None:intergenic 30.0%
CCCCTTATAATAACAAAAAC+TGG + chr1_4:57457607-57457626 None:intergenic 30.0%
!! ACTGTTCTGTGTTCTTGTTT+TGG - chr1_4:57451391-57451410 Msa0172400:three_prime_UTR 35.0%
AAGATCACACGGTTACAATT+AGG - chr1_4:57451901-57451920 Msa0172400:intron 35.0%
GAATAAACATGGACAACGTA+TGG - chr1_4:57452666-57452685 Msa0172400:intron 35.0%
ACAACGTATGGTAATCAATC+AGG - chr1_4:57452678-57452697 Msa0172400:intron 35.0%
CAACGTATGGTAATCAATCA+GGG - chr1_4:57452679-57452698 Msa0172400:intron 35.0%
TGTTAATTCCCCCAAACAAA+AGG - chr1_4:57452946-57452965 Msa0172400:intron 35.0%
CATCAATGATCTTAATCGGA+TGG + chr1_4:57453169-57453188 None:intergenic 35.0%
! TTTCTAAAGGATCTAACTGG+TGG + chr1_4:57453389-57453408 None:intergenic 35.0%
TCCTTCACAATTGACAATTG+TGG - chr1_4:57453590-57453609 Msa0172400:intron 35.0%
CCTTCACAATTGACAATTGT+GGG - chr1_4:57453591-57453610 Msa0172400:intron 35.0%
! CATAAAGTCCTTGGTAACTT+GGG + chr1_4:57453618-57453637 None:intergenic 35.0%
! TCATAAAGTCCTTGGTAACT+TGG + chr1_4:57453619-57453638 None:intergenic 35.0%
GCTTTCATTCATAAAGTCCT+TGG + chr1_4:57453627-57453646 None:intergenic 35.0%
CATAATCAGAACCGTTCAAA+AGG + chr1_4:57453661-57453680 None:intergenic 35.0%
CCATCTTTGTCTTCATATGT+TGG + chr1_4:57453686-57453705 None:intergenic 35.0%
CCAACATATGAAGACAAAGA+TGG - chr1_4:57453683-57453702 Msa0172400:intron 35.0%
ATGAAGACAAAGATGGTGAT+TGG - chr1_4:57453690-57453709 Msa0172400:intron 35.0%
!! TATGTGGGCTTTGAAATTTG+GGG + chr1_4:57453764-57453783 None:intergenic 35.0%
!! TTAATTTAGTGGGTTTGGTG+GGG + chr1_4:57453794-57453813 None:intergenic 35.0%
!! CTTTAATTTAGTGGGTTTGG+TGG + chr1_4:57453796-57453815 None:intergenic 35.0%
! TTAGGGGGCTTTAATTTAGT+GGG + chr1_4:57453804-57453823 None:intergenic 35.0%
! ATTAGGGGGCTTTAATTTAG+TGG + chr1_4:57453805-57453824 None:intergenic 35.0%
ATGTGAGGTGAAAAAAAGAG+GGG + chr1_4:57456849-57456868 None:intergenic 35.0%
!! TGATGATGAATGGATTGGTT+CGG + chr1_4:57456924-57456943 None:intergenic 35.0%
! CGACTTAATCTGATGATGAA+TGG + chr1_4:57456934-57456953 None:intergenic 35.0%
TCTGCAAAAATTGCGTAGAT+TGG + chr1_4:57457015-57457034 None:intergenic 35.0%
!! TTGCATTTTTCAACTGCTCT+TGG + chr1_4:57457326-57457345 None:intergenic 35.0%
! ATTTTTGTCGGAACACAACA+AGG + chr1_4:57457365-57457384 None:intergenic 35.0%
!! AACCAGAAGTGGATTTTTGT+CGG + chr1_4:57457377-57457396 None:intergenic 35.0%
ATGTTTCCAGTTATCATTGC+TGG - chr1_4:57457682-57457701 Msa0172400:CDS 35.0%
ACAAATCCAGCAATGATAAC+TGG + chr1_4:57457691-57457710 None:intergenic 35.0%
! ATTAAATGTGGACTGCACAG+TGG + chr1_4:57451432-57451451 None:intergenic 40.0%
! TATTTGCTACGCCATGACAA+AGG + chr1_4:57451530-57451549 None:intergenic 40.0%
TTGTCATGGCGTAGCAAATA+TGG - chr1_4:57451530-57451549 Msa0172400:three_prime_UTR 40.0%
TATGGTAATCAATCAGGGCA+TGG - chr1_4:57452684-57452703 Msa0172400:intron 40.0%
!!! GATTTTTTCTGCGGATAGCA+TGG - chr1_4:57452847-57452866 Msa0172400:intron 40.0%
TCAACGACGATAGAGAATCA+TGG - chr1_4:57453223-57453242 Msa0172400:intron 40.0%
AATTTGTAGGGCACAAGTTG+TGG - chr1_4:57453358-57453377 Msa0172400:intron 40.0%
ATTTGTAGGGCACAAGTTGT+GGG - chr1_4:57453359-57453378 Msa0172400:intron 40.0%
! TGGGAATGGAGCATTTGTTA+AGG - chr1_4:57453472-57453491 Msa0172400:intron 40.0%
! AGCATTTGTTAAGGTGAGCA+TGG - chr1_4:57453481-57453500 Msa0172400:intron 40.0%
TTTGTTAAGGTGAGCATGGA+TGG - chr1_4:57453485-57453504 Msa0172400:intron 40.0%
CCCACAATTGTCAATTGTGA+AGG + chr1_4:57453594-57453613 None:intergenic 40.0%
!! TAATTTAGTGGGTTTGGTGG+GGG + chr1_4:57453793-57453812 None:intergenic 40.0%
!! GGGCTTTAATTTAGTGGGTT+TGG + chr1_4:57453799-57453818 None:intergenic 40.0%
!!! CTTGAGGGCTTTTGATTTGT+GGG - chr1_4:57456713-57456732 Msa0172400:intron 40.0%
! ATGTGGGCCTTTTAATCTTG+TGG - chr1_4:57456745-57456764 Msa0172400:intron 40.0%
! TTTTAAGCCCCATTGCTATG+TGG - chr1_4:57456770-57456789 Msa0172400:intron 40.0%
TTTAAGCCCCATTGCTATGT+GGG - chr1_4:57456771-57456790 Msa0172400:intron 40.0%
!! CTAGAGGTGTCAATATCATG+GGG + chr1_4:57456817-57456836 None:intergenic 40.0%
!! CCTAGAGGTGTCAATATCAT+GGG + chr1_4:57456818-57456837 None:intergenic 40.0%
!! TCCTAGAGGTGTCAATATCA+TGG + chr1_4:57456819-57456838 None:intergenic 40.0%
!! GATGATGAATGGATTGGTTC+GGG + chr1_4:57456923-57456942 None:intergenic 40.0%
AAGCGTCTTCGCATAATGAA+AGG - chr1_4:57457171-57457190 Msa0172400:intron 40.0%
TTCCGACAAAAATCCACTTC+TGG - chr1_4:57457372-57457391 Msa0172400:intron 40.0%
GCAGATTTATCAACCAGAAG+TGG + chr1_4:57457388-57457407 None:intergenic 40.0%
! GTTGATAAATCTGCATCCCA+AGG - chr1_4:57457394-57457413 Msa0172400:intron 40.0%
AGTAAGGTCGAGGCTAATAT+AGG - chr1_4:57457432-57457451 Msa0172400:intron 40.0%
ATTATAAGTCAAGCACACGC+TGG + chr1_4:57457655-57457674 None:intergenic 40.0%
ACAACCCTGAGCAACATAAC+TGG - chr1_4:57451456-57451475 Msa0172400:three_prime_UTR 45.0%
CAACCCTGAGCAACATAACT+GGG - chr1_4:57451457-57451476 Msa0172400:three_prime_UTR 45.0%
AGTCCCAGTTATGTTGCTCA+GGG + chr1_4:57451463-57451482 None:intergenic 45.0%
AAGTCCCAGTTATGTTGCTC+AGG + chr1_4:57451464-57451483 None:intergenic 45.0%
! TGAGTGCATTTTGCGCAACA+TGG - chr1_4:57451492-57451511 Msa0172400:three_prime_UTR 45.0%
! GAGTGCATTTTGCGCAACAT+GGG - chr1_4:57451493-57451512 Msa0172400:three_prime_UTR 45.0%
!! AAGTATTGGATGGTGGCTGA+AGG - chr1_4:57452784-57452803 Msa0172400:intron 45.0%
! TTTTCTGCGGATAGCATGGT+TGG - chr1_4:57452851-57452870 Msa0172400:intron 45.0%
GCATAAATTCGTGCAGTCGA+TGG - chr1_4:57453262-57453281 Msa0172400:intron 45.0%
! GAAGCAGATAAGAGCACAAG+TGG - chr1_4:57453446-57453465 Msa0172400:intron 45.0%
! ATAAGAGCACAAGTGGTGGT+GGG - chr1_4:57453453-57453472 Msa0172400:intron 45.0%
TGGTGCACCATATCTACGTA+AGG - chr1_4:57453505-57453524 Msa0172400:intron 45.0%
TGCACCATATCTACGTAAGG+TGG - chr1_4:57453508-57453527 Msa0172400:intron 45.0%
AGGTCCACCTTACGTAGATA+TGG + chr1_4:57453515-57453534 None:intergenic 45.0%
GATGGTGATTGGATGCTTGT+TGG - chr1_4:57453701-57453720 Msa0172400:intron 45.0%
!! TGCTTGTTGGTGATGTTCCT+TGG - chr1_4:57453714-57453733 Msa0172400:intron 45.0%
!! GCTTGTTGGTGATGTTCCTT+GGG - chr1_4:57453715-57453734 Msa0172400:intron 45.0%
!!! TGGTGGGGGCTTTTTTATGT+GGG + chr1_4:57453779-57453798 None:intergenic 45.0%
!!! TTGGTGGGGGCTTTTTTATG+TGG + chr1_4:57453780-57453799 None:intergenic 45.0%
CCACAAATCAAAAGCCCTCA+AGG + chr1_4:57456715-57456734 None:intergenic 45.0%
!!! CCTTGAGGGCTTTTGATTTG+TGG - chr1_4:57456712-57456731 Msa0172400:intron 45.0%
TTTGTGGGCTTGCATGAATG+TGG - chr1_4:57456728-57456747 Msa0172400:intron 45.0%
TTGTGGGCTTGCATGAATGT+GGG - chr1_4:57456729-57456748 Msa0172400:intron 45.0%
!! ATCATGGGGCCTTCAAGTTT+TGG + chr1_4:57456803-57456822 None:intergenic 45.0%
CCCATGATATTGACACCTCT+AGG - chr1_4:57456815-57456834 Msa0172400:intron 45.0%
!! ATTGGTTCGGGTCAGATCAA+CGG + chr1_4:57456911-57456930 None:intergenic 45.0%
TCGCATAATGAAAGGTTCCG+AGG - chr1_4:57457179-57457198 Msa0172400:intron 45.0%
ATGAAAGGTTCCGAGGCAAT+TGG - chr1_4:57457186-57457205 Msa0172400:intron 45.0%
TGAAAGGTTCCGAGGCAATT+GGG - chr1_4:57457187-57457206 Msa0172400:intron 45.0%
TTTACCTAGCCCAATTGCCT+CGG + chr1_4:57457199-57457218 None:intergenic 45.0%
GCACATACTCTGTCACGTAT+TGG + chr1_4:57457736-57457755 None:intergenic 45.0%
!!! CTATTTTAAAATTTATATAT+TGG - chr1_4:57451690-57451709 Msa0172400:three_prime_UTR 5.0%
!! AAATAAAAAAAATAAAGTAT+TGG - chr1_4:57452770-57452789 Msa0172400:intron 5.0%
!! TAAGAAAAAAATTATAATAT+GGG + chr1_4:57453891-57453910 None:intergenic 5.0%
!! TTAAGAAAAAAATTATAATA+TGG + chr1_4:57453892-57453911 None:intergenic 5.0%
!!! GTTATTTTTTAAAATTAAAT+CGG + chr1_4:57454486-57454505 None:intergenic 5.0%
!!! GTTATTTTTAAAAATTAAAT+CGG - chr1_4:57456344-57456363 Msa0172400:intron 5.0%
ATGCAGACCAGCCTTTGTCA+TGG - chr1_4:57451516-57451535 Msa0172400:three_prime_UTR 50.0%
CATGGCGTAGCAAATATGGC+TGG - chr1_4:57451534-57451553 Msa0172400:three_prime_UTR 50.0%
TTCTTGTGGCCGTTGCTTGA+GGG - chr1_4:57451595-57451614 Msa0172400:three_prime_UTR 50.0%
CCAAGCATACCCTCAAGCAA+CGG + chr1_4:57451607-57451626 None:intergenic 50.0%
GGTAATCAATCAGGGCATGG+TGG - chr1_4:57452687-57452706 Msa0172400:intron 50.0%
AATCAATCAGGGCATGGTGG+AGG - chr1_4:57452690-57452709 Msa0172400:intron 50.0%
! TGGATGGTGGCTGAAGGAAT+AGG - chr1_4:57452790-57452809 Msa0172400:intron 50.0%
TGGTGGCTGAAGGAATAGGT+TGG - chr1_4:57452794-57452813 Msa0172400:intron 50.0%
! CGGATAGCATGGTTGGTTGT+CGG - chr1_4:57452858-57452877 Msa0172400:intron 50.0%
! GGATAGCATGGTTGGTTGTC+GGG - chr1_4:57452859-57452878 Msa0172400:intron 50.0%
GTGACACGCACACAAGACAA+GGG + chr1_4:57453003-57453022 None:intergenic 50.0%
TGAATGCGTGCAGTCGTTGA+CGG + chr1_4:57453127-57453146 None:intergenic 50.0%
! GCAGATAAGAGCACAAGTGG+TGG - chr1_4:57453449-57453468 Msa0172400:intron 50.0%
! GATAAGAGCACAAGTGGTGG+TGG - chr1_4:57453452-57453471 Msa0172400:intron 50.0%
TTGTGGGTCCCAAGTTACCA+AGG - chr1_4:57453607-57453626 Msa0172400:intron 50.0%
TGTTCCTTGGGAGTGAGTAG+AGG - chr1_4:57453727-57453746 Msa0172400:intron 50.0%
TGCATCCCAAGGAAGCAAGT+TGG - chr1_4:57457405-57457424 Msa0172400:intron 50.0%
! TCGAGCCAACTTGCTTCCTT+GGG + chr1_4:57457413-57457432 None:intergenic 50.0%
!! GAAGCAAGTTGGCTCGAGTA+AGG - chr1_4:57457416-57457435 Msa0172400:intron 50.0%
TGCTACGCCATGACAAAGGC+TGG + chr1_4:57451526-57451545 None:intergenic 55.0%
TATGGCTGGACTGCCTTCTG+TGG - chr1_4:57451548-57451567 Msa0172400:three_prime_UTR 55.0%
!! CCACAAGAAGCTTGGCCTTG+AGG + chr1_4:57451584-57451603 None:intergenic 55.0%
CCTCAAGGCCAAGCTTCTTG+TGG - chr1_4:57451581-57451600 Msa0172400:three_prime_UTR 55.0%
AGCAACGGCCACAAGAAGCT+TGG + chr1_4:57451592-57451611 None:intergenic 55.0%
CTTCTTGTGGCCGTTGCTTG+AGG - chr1_4:57451594-57451613 Msa0172400:three_prime_UTR 55.0%
! CCGTTGCTTGAGGGTATGCT+TGG - chr1_4:57451604-57451623 Msa0172400:three_prime_UTR 55.0%
GGTGACACGCACACAAGACA+AGG + chr1_4:57453004-57453023 None:intergenic 55.0%
GTAGGGCACAAGTTGTGGGA+TGG - chr1_4:57453363-57453382 Msa0172400:intron 55.0%
!! AGCACAAGTGGTGGTGGGAA+TGG - chr1_4:57453458-57453477 Msa0172400:intron 55.0%
GACACCTCTACTCACTCCCA+AGG + chr1_4:57453734-57453753 None:intergenic 55.0%
!!! TTTTGGGGCTAAGGGCCTTG+AGG - chr1_4:57456697-57456716 Msa0172400:intron 55.0%
! TTTGGGGCTAAGGGCCTTGA+GGG - chr1_4:57456698-57456717 Msa0172400:intron 55.0%
!! AGAGGGGTACTCACTCCTAG+AGG + chr1_4:57456833-57456852 None:intergenic 55.0%
! CTCGAGCCAACTTGCTTCCT+TGG + chr1_4:57457414-57457433 None:intergenic 55.0%
!! AGTTGGCTCGAGTAAGGTCG+AGG - chr1_4:57457422-57457441 Msa0172400:intron 55.0%
ACTGCCTTCTGTGGACCTGC+TGG - chr1_4:57451557-57451576 Msa0172400:three_prime_UTR 60.0%
CTGCCTTCTGTGGACCTGCT+GGG - chr1_4:57451558-57451577 Msa0172400:three_prime_UTR 60.0%
! GCACACAAGACAAGGGCGTG+TGG + chr1_4:57452996-57453015 None:intergenic 60.0%
CCAGCGCATCTGACAGCTCT+TGG + chr1_4:57453556-57453575 None:intergenic 60.0%
CCAAGAGCTGTCAGATGCGC+TGG - chr1_4:57453553-57453572 Msa0172400:intron 60.0%
AGCCCCATTGCTATGTGGGC+TGG - chr1_4:57456775-57456794 Msa0172400:intron 60.0%
GCCCCATTGCTATGTGGGCT+GGG - chr1_4:57456776-57456795 Msa0172400:intron 60.0%
CCCCAGCCCACATAGCAATG+GGG + chr1_4:57456780-57456799 None:intergenic 60.0%
! CCCCATTGCTATGTGGGCTG+GGG - chr1_4:57456777-57456796 Msa0172400:intron 60.0%
GCCCCAGCCCACATAGCAAT+GGG + chr1_4:57456781-57456800 None:intergenic 60.0%
GGGCTGGGGCCAAAACTTGA+AGG - chr1_4:57456791-57456810 Msa0172400:intron 60.0%
GGTTCCGAGGCAATTGGGCT+AGG - chr1_4:57457192-57457211 Msa0172400:intron 60.0%
AGGCCCAGCAGGTCCACAGA+AGG + chr1_4:57451564-57451583 None:intergenic 65.0%
TGTGGACCTGCTGGGCCTCA+AGG - chr1_4:57451566-57451585 Msa0172400:three_prime_UTR 65.0%
GGCCCCAGCCCACATAGCAA+TGG + chr1_4:57456782-57456801 None:intergenic 65.0%
!! GCTTGGCCTTGAGGCCCAGC+AGG + chr1_4:57451575-57451594 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 57451383 57457829 57451383 ID=Msa0172400;Name=Msa0172400
chr1_4 mRNA 57451383 57457829 57451383 ID=Msa0172400-mRNA-1;Parent=Msa0172400;Name=Msa0172400-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0.25|0.4|1|1|1|5|471|185
chr1_4 exon 57451383 57451891 57451383 ID=Msa0172400-mRNA-1:exon:17586;Parent=Msa0172400-mRNA-1
chr1_4 exon 57451999 57452060 57451999 ID=Msa0172400-mRNA-1:exon:17585;Parent=Msa0172400-mRNA-1
chr1_4 exon 57455630 57455845 57455630 ID=Msa0172400-mRNA-1:exon:17584;Parent=Msa0172400-mRNA-1
chr1_4 exon 57457326 57457333 57457326 ID=Msa0172400-mRNA-1:exon:17583;Parent=Msa0172400-mRNA-1
chr1_4 exon 57457596 57457829 57457596 ID=Msa0172400-mRNA-1:exon:17582;Parent=Msa0172400-mRNA-1
chr1_4 CDS 57457596 57457829 57457596 ID=Msa0172400-mRNA-1:cds;Parent=Msa0172400-mRNA-1
chr1_4 CDS 57457326 57457333 57457326 ID=Msa0172400-mRNA-1:cds;Parent=Msa0172400-mRNA-1
chr1_4 CDS 57455630 57455845 57455630 ID=Msa0172400-mRNA-1:cds;Parent=Msa0172400-mRNA-1
chr1_4 CDS 57451999 57452060 57451999 ID=Msa0172400-mRNA-1:cds;Parent=Msa0172400-mRNA-1
chr1_4 CDS 57451854 57451891 57451854 ID=Msa0172400-mRNA-1:cds;Parent=Msa0172400-mRNA-1
chr1_4 three_prime_UTR 57451383 57451853 57451383 ID=Msa0172400-mRNA-1:three_prime_utr;Parent=Msa0172400-mRNA-1
Gene Sequence

>Msa0172400

ATGTTGTCACTGTTCTGTGTTCTTGTTTTGGCAACTGCTCAAAGTGCCACTGTGCAGTCCACATTTAATTTATACAACCCTGAGCAACATAACTGGGACTTACGTGCAGTGAGTGCATTTTGCGCAACATGGGATGCAGACCAGCCTTTGTCATGGCGTAGCAAATATGGCTGGACTGCCTTCTGTGGACCTGCTGGGCCTCAAGGCCAAGCTTCTTGTGGCCGTTGCTTGAGGGTTAGAAGGGCACAAGTTGTGGGATGGCCACCAGTTAGATCCTTTAGAAAGAACATAGTAAATGTTCAAAAGAGCAATAATGAATCAGAAGCAGATAAGAGCACAAGTGGTGGTGGGAATGGAGCATTTGTTAAGGTGAGCATGGATGGTGCACCATATCTACGTAAGGTGGACCTTAAATTGTACAAGAGTTACCAAGAGCTGTCAGATGCGCTGGCTAAAATAATGTTTGTTCAATCGTGCAAGCGTCTTCGCATAATGAAAGGTTCCGAGGCAATTGGGCTAGCACCAAGAGCAGTTGAAAAATGCAAGAACAGAAGCTGA
Protein sequence

>Msa0172400

MLSLFCVLVLATAQSATVQSTFNLYNPEQHNWDLRAVSAFCATWDADQPLSWRSKYGWTAFCGPAGPQGQASCGRCLRVRRAQVVGWPPVRSFRKNIVNVQKSNNESEADKSTSGGGNGAFVKVSMDGAPYLRKVDLKLYKSYQELSDALAKIMFVQSCKRLRIMKGSEAIGLAPRAVEKCKNRS*