AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0206130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0206130 MtrunA17_Chr2g0304721 82.687 335 39 4 1 316 183 517 0.0 537
Msa0206130 MtrunA17_Chr2g0305211 68.047 338 86 2 1 316 183 520 8.74e-166 471
Msa0206130 MtrunA17_Chr2g0305231 68.047 338 86 2 1 316 183 520 8.74e-166 471
Msa0206130 MtrunA17_Chr2g0304651 67.160 338 87 3 1 316 183 518 1.14e-157 451
Msa0206130 MtrunA17_Chr4g0070101 38.855 332 178 8 1 310 191 519 3.03e-62 206
Msa0206130 MtrunA17_Chr2g0301661 38.253 332 181 5 1 310 190 519 4.49e-59 197
Msa0206130 MtrunA17_Chr2g0301471 38.253 332 181 5 1 310 190 519 8.50e-56 189
Msa0206130 MtrunA17_Chr4g0070111 36.299 281 157 5 51 311 1 279 9.63e-54 177
Msa0206130 MtrunA17_Chr2g0286671 40.206 97 57 1 214 310 5 100 5.80e-18 78.2
Msa0206130 MtrunA17_Chr2g0286681 40.476 84 49 1 106 189 19 101 1.96e-17 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0206130 AT1G19250.1 38.623 334 177 7 1 310 189 518 4.67e-67 219
Msa0206130 AT5G45180.1 33.123 317 142 9 1 291 187 459 6.92e-43 154

Find 80 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACTGTCAATGTAATTT+TGG 0.131877 2_1:+34810628 None:intergenic
TCTCCCAGATTTCAACATTC+TGG 0.210934 2_1:-34811244 Msa0206130:CDS
AATAACTACATCTGTTTCTA+AGG 0.224263 2_1:+34810710 None:intergenic
CCCGGAGAACACTTCCTTCT+TGG 0.233331 2_1:-34811165 Msa0206130:CDS
TGGAGAAGGATGTTAAATTA+TGG 0.235292 2_1:-34809714 Msa0206130:CDS
ACACTTCCTTCTTGGCCTTT+TGG 0.237410 2_1:-34811157 Msa0206130:CDS
ATATAGATGCTTATGGCATT+AGG 0.278308 2_1:+34811961 None:intergenic
AGGTAAGTTTATGCATTCTA+TGG 0.279876 2_1:-34811568 Msa0206130:exon
GAAATATCTGCATGTCAAAT+TGG 0.294249 2_1:-34810846 Msa0206130:CDS
CAAAGTTTGTAAGGACATAT+TGG 0.315462 2_1:+34811849 None:intergenic
GAAGTTGAGTTTGCACTCTT+AGG 0.320195 2_1:+34811917 None:intergenic
GACTGAATGTTTACAATAAC+AGG 0.322466 2_1:-34811688 Msa0206130:five_prime_UTR
TATAGATGCTTATGGCATTA+GGG 0.324531 2_1:+34811962 None:intergenic
CTCCCAGATTTCAACATTCT+GGG 0.325483 2_1:-34811243 Msa0206130:CDS
GCGGAGCTATTTGAACCATA+TGG 0.339270 2_1:-34809562 Msa0206130:CDS
AAAATTACATTGACAGTTCA+AGG 0.349499 2_1:-34810626 Msa0206130:CDS
ATGCCCAGAATGTTGAAATC+TGG 0.349568 2_1:+34811240 None:intergenic
ACCATTTGCATTTGCGCATT+CGG 0.352274 2_1:+34811449 None:intergenic
TCTTGAAGAAAACTATGATT+AGG 0.356221 2_1:+34810867 None:intergenic
CTTTGCATTGGGAAATATAG+TGG 0.362241 2_1:-34811642 Msa0206130:five_prime_UTR
ATTCTTGCCACAGGATATAA+AGG 0.366577 2_1:-34810690 Msa0206130:intron
AATAAGAGAGTTACAGTAGT+AGG 0.371604 2_1:-34811501 Msa0206130:CDS
TATAAGCTGTTTATCCAAAC+GGG 0.388963 2_1:-34811941 Msa0206130:five_prime_UTR
GGGAGGAACTCAGGAATGTT+TGG 0.394469 2_1:+34811615 None:intergenic
GGAGAAGGATGTTAAATTAT+GGG 0.394999 2_1:-34809713 Msa0206130:CDS
TTGCACTCTTAGGCCCCGTT+TGG 0.404935 2_1:+34811927 None:intergenic
GCCAAGAAGGAAGTGTTCTC+CGG 0.407386 2_1:+34811164 None:intergenic
ACAGCTTATATAGATGCTTA+TGG 0.409130 2_1:+34811954 None:intergenic
AAGTTGCCACTGAAGAAGTA+TGG 0.417922 2_1:-34810897 Msa0206130:CDS
CGGAGCTATTTGAACCATAT+GGG 0.422701 2_1:-34809561 Msa0206130:CDS
TTGTTGAAACATATCTGAAA+TGG 0.425780 2_1:-34810920 Msa0206130:CDS
AGCTTTAGCTTCTGCAAAGA+AGG 0.430511 2_1:-34810759 Msa0206130:CDS
TTATAATCAGTGGGCCCATA+TGG 0.433595 2_1:+34809547 None:intergenic
ATATAAGCTGTTTATCCAAA+CGG 0.433607 2_1:-34811942 Msa0206130:five_prime_UTR
AAGTTAAAGCAAAGTTTGTA+AGG 0.436012 2_1:+34811840 None:intergenic
GCATTGTTACTTGTGGCATA+TGG 0.438868 2_1:-34809645 Msa0206130:CDS
AATTATTGTTGTATTGCAAA+GGG 0.439126 2_1:+34811718 None:intergenic
AGTTTGTTATTCTTTGCATT+GGG 0.451815 2_1:-34811653 Msa0206130:five_prime_UTR
GTTCAAGGATTTCTCTCTAC+AGG 0.451911 2_1:-34810611 Msa0206130:intron
GGCCACTTTACTTTCACCAT+TGG 0.455226 2_1:-34811136 Msa0206130:intron
GAAAATTGAATATATTCTGC+GGG 0.461372 2_1:+34811874 None:intergenic
GAGTTTGTTATTCTTTGCAT+TGG 0.462117 2_1:-34811654 Msa0206130:five_prime_UTR
TTCTATGGATTACTCTGCCT+TGG 0.462649 2_1:-34811553 Msa0206130:CDS
TTGTGATTTCTTTATCTCGA+TGG 0.463038 2_1:+34810782 None:intergenic
AACATTCCTGAGTTCCTCCC+TGG 0.466304 2_1:-34811612 Msa0206130:five_prime_UTR
CCTGAGTTCCTCCCTGGAAA+AGG 0.469202 2_1:-34811606 Msa0206130:five_prime_UTR
TGCCCAGAATGTTGAAATCT+GGG 0.478870 2_1:+34811241 None:intergenic
TATGGGCCCACTGATTATAA+TGG 0.482729 2_1:-34809544 Msa0206130:CDS
AAATTATTGTTGTATTGCAA+AGG 0.484351 2_1:+34811717 None:intergenic
AACAAGACCATTATAATCAG+TGG 0.502752 2_1:+34809537 None:intergenic
TCCGAATGCGCAAATGCAAA+TGG 0.502988 2_1:-34811450 Msa0206130:intron
AGAGTTACAGTAGTAGGCTC+AGG 0.504894 2_1:-34811495 Msa0206130:CDS
AAAAGACCCTTCTTTCTCCT+CGG 0.506769 2_1:+34809586 None:intergenic
AACATTCTGGGCATTCGTCT+CGG 0.507223 2_1:-34811231 Msa0206130:CDS
CTAGAAAATGTGCTAACCAT+TGG 0.509768 2_1:+34809770 None:intergenic
GTGAAAGTAAAGTGGCCAAA+AGG 0.517743 2_1:+34811142 None:intergenic
AAGTGGCCAAAAGGCCAAGA+AGG 0.525815 2_1:+34811151 None:intergenic
TCGGATTACACTTCATGTCT+CGG 0.532826 2_1:+34809605 None:intergenic
GTTCTATTTATAATCGTGCA+AGG 0.542558 2_1:+34811279 None:intergenic
CCAAGAAGGAAGTGTTCTCC+GGG 0.546254 2_1:+34811165 None:intergenic
ATAACTACATCTGTTTCTAA+GGG 0.546830 2_1:+34810711 None:intergenic
AGCAGCAGTTTCTTTGTCCA+AGG 0.547408 2_1:+34811536 None:intergenic
GTGTAATCCGAGGAGAAAGA+AGG 0.551929 2_1:-34809593 Msa0206130:CDS
ATTGCCTAGTATAAAAGAGA+TGG 0.565732 2_1:-34809734 Msa0206130:CDS
TACCAATGGTGAAAGTAAAG+TGG 0.578127 2_1:+34811134 None:intergenic
TGAAAATTGAATATATTCTG+CGG 0.584707 2_1:+34811873 None:intergenic
AAGATATTAACATGCAATCA+CGG 0.599410 2_1:-34811753 Msa0206130:five_prime_UTR
GGCAATGATAGGATATGCTG+AGG 0.603017 2_1:-34809827 Msa0206130:intron
AGATCATGCATTGTTACTTG+TGG 0.603581 2_1:-34809652 Msa0206130:CDS
TTCTATTTATAATCGTGCAA+GGG 0.618260 2_1:+34811280 None:intergenic
ACAAGACCATTATAATCAGT+GGG 0.632491 2_1:+34809538 None:intergenic
TAGTATAAAAGAGATGGAGA+AGG 0.639486 2_1:-34809728 Msa0206130:CDS
TGTAATCCGAGGAGAAAGAA+GGG 0.645189 2_1:-34809592 Msa0206130:CDS
ACTAGTCCATACTTCTTCAG+TGG 0.645916 2_1:+34810891 None:intergenic
CTAATGAAATGAGATGCCAA+TGG 0.660576 2_1:-34809786 Msa0206130:CDS
GAGACATGAAGTGTAATCCG+AGG 0.661308 2_1:-34809603 Msa0206130:CDS
TTATAAATAGAACTTCACAC+TGG 0.667245 2_1:-34811269 Msa0206130:CDS
AAAATTGAATATATTCTGCG+GGG 0.669539 2_1:+34811875 None:intergenic
GCAATGATAGGATATGCTGA+GGG 0.702690 2_1:-34809826 Msa0206130:intron
ATAAGCTGTTTATCCAAACG+GGG 0.708505 2_1:-34811940 Msa0206130:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATT+TGG - chr2_1:34811084-34811103 Msa0206130:intron 0.0%
!! ATATATATATATATATATTT+GGG - chr2_1:34811085-34811104 Msa0206130:intron 0.0%
!! TCTTACAAAAATATATAAAT+TGG + chr2_1:34810535-34810554 None:intergenic 10.0%
!! ATCTTTCAAATATATATATA+GGG + chr2_1:34811231-34811250 None:intergenic 10.0%
!! AATCTTTCAAATATATATAT+AGG + chr2_1:34811232-34811251 None:intergenic 10.0%
!! ATATATATTTGAAAGATTAA+CGG - chr2_1:34811234-34811253 Msa0206130:CDS 10.0%
!!! TATTTTTGATATGTATTTGT+AGG + chr2_1:34811015-34811034 None:intergenic 15.0%
!!! AATATTCCAGAAAATTTTAT+TGG - chr2_1:34811868-34811887 Msa0206130:five_prime_UTR 15.0%
!! TGAAAATTGAATATATTCTG+CGG + chr2_1:34809673-34809692 None:intergenic 20.0%
!! AATTATTGTTGTATTGCAAA+GGG + chr2_1:34809828-34809847 None:intergenic 20.0%
!! AAATTATTGTTGTATTGCAA+AGG + chr2_1:34809829-34809848 None:intergenic 20.0%
!!! TGAATATGCTTTTAAGTTTT+TGG + chr2_1:34810882-34810901 None:intergenic 20.0%
!!! TCAATGTAATTTTGGAATAT+TGG + chr2_1:34810910-34810929 None:intergenic 20.0%
!! CAAATCATACATAAGAAAAT+AGG + chr2_1:34810990-34811009 None:intergenic 20.0%
!!! CTATTTTCTTATGTATGATT+TGG - chr2_1:34810988-34811007 Msa0206130:intron 20.0%
! ATATAAGCTGTTTATCCAAA+CGG - chr2_1:34809601-34809620 Msa0206130:CDS 25.0%
! AAAATTGAATATATTCTGCG+GGG + chr2_1:34809671-34809690 None:intergenic 25.0%
! GAAAATTGAATATATTCTGC+GGG + chr2_1:34809672-34809691 None:intergenic 25.0%
! AAGTTAAAGCAAAGTTTGTA+AGG + chr2_1:34809706-34809725 None:intergenic 25.0%
! AAGATATTAACATGCAATCA+CGG - chr2_1:34809790-34809809 Msa0206130:CDS 25.0%
!!! AGTTTGTTATTCTTTGCATT+GGG - chr2_1:34809890-34809909 Msa0206130:intron 25.0%
! TTCAATCCATTAATTTCTAC+AGG - chr2_1:34810231-34810250 Msa0206130:intron 25.0%
! TTCTATTTATAATCGTGCAA+GGG + chr2_1:34810266-34810285 None:intergenic 25.0%
! TTATAAATAGAACTTCACAC+TGG - chr2_1:34810274-34810293 Msa0206130:intron 25.0%
!! CAATCTAAATTTTCAGAGAT+GGG - chr2_1:34810588-34810607 Msa0206130:intron 25.0%
!! AATCTAAATTTTCAGAGATG+GGG - chr2_1:34810589-34810608 Msa0206130:intron 25.0%
! TTGTTGAAACATATCTGAAA+TGG - chr2_1:34810623-34810642 Msa0206130:CDS 25.0%
! TCTTGAAGAAAACTATGATT+AGG + chr2_1:34810679-34810698 None:intergenic 25.0%
!! TTCTTTTACTTGGTCAAAAA+AGG + chr2_1:34810737-34810756 None:intergenic 25.0%
!!! TTTTTTGACCAAGTAAAAGA+AGG - chr2_1:34810736-34810755 Msa0206130:CDS 25.0%
!!! ATAACTACATCTGTTTCTAA+GGG + chr2_1:34810835-34810854 None:intergenic 25.0%
!!! AATAACTACATCTGTTTCTA+AGG + chr2_1:34810836-34810855 None:intergenic 25.0%
!! TTGAACTGTCAATGTAATTT+TGG + chr2_1:34810918-34810937 None:intergenic 25.0%
! AAAATTACATTGACAGTTCA+AGG - chr2_1:34810917-34810936 Msa0206130:CDS 25.0%
! AAATTCTCTATACTAATCCA+TGG - chr2_1:34811651-34811670 Msa0206130:five_prime_UTR 25.0%
!! TGATCTCCAATAAAATTTTC+TGG + chr2_1:34811877-34811896 None:intergenic 25.0%
TATAGATGCTTATGGCATTA+GGG + chr2_1:34809584-34809603 None:intergenic 30.0%
ATATAGATGCTTATGGCATT+AGG + chr2_1:34809585-34809604 None:intergenic 30.0%
ACAGCTTATATAGATGCTTA+TGG + chr2_1:34809592-34809611 None:intergenic 30.0%
TATAAGCTGTTTATCCAAAC+GGG - chr2_1:34809602-34809621 Msa0206130:CDS 30.0%
CAAAGTTTGTAAGGACATAT+TGG + chr2_1:34809697-34809716 None:intergenic 30.0%
GACTGAATGTTTACAATAAC+AGG - chr2_1:34809855-34809874 Msa0206130:intron 30.0%
!! GAGTTTGTTATTCTTTGCAT+TGG - chr2_1:34809889-34809908 Msa0206130:intron 30.0%
ACTTACCTGTAAAAACTTCT+GGG + chr2_1:34809963-34809982 None:intergenic 30.0%
AACTTACCTGTAAAAACTTC+TGG + chr2_1:34809964-34809983 None:intergenic 30.0%
AGGTAAGTTTATGCATTCTA+TGG - chr2_1:34809975-34809994 Msa0206130:intron 30.0%
AATAAGAGAGTTACAGTAGT+AGG - chr2_1:34810042-34810061 Msa0206130:intron 30.0%
GTGATTCCTGTAGAAATTAA+TGG + chr2_1:34810240-34810259 None:intergenic 30.0%
GTTCTATTTATAATCGTGCA+AGG + chr2_1:34810267-34810286 None:intergenic 30.0%
GTATCTTACATTCTTACCAA+TGG + chr2_1:34810426-34810445 None:intergenic 30.0%
! GCAATCTAAATTTTCAGAGA+TGG - chr2_1:34810587-34810606 Msa0206130:intron 30.0%
GAAATATCTGCATGTCAAAT+TGG - chr2_1:34810697-34810716 Msa0206130:intron 30.0%
TTGTGATTTCTTTATCTCGA+TGG + chr2_1:34810764-34810783 None:intergenic 30.0%
GCAAAGTTACAACTAAAAAG+TGG + chr2_1:34811171-34811190 None:intergenic 30.0%
CTAAAAGACACCTTCATAAA+AGG - chr2_1:34811190-34811209 Msa0206130:CDS 30.0%
AATTTGAGAGTGACTTACAA+AGG + chr2_1:34811296-34811315 None:intergenic 30.0%
TAAATTAAGTCTCTCGTCAT+GGG + chr2_1:34811506-34811525 None:intergenic 30.0%
AAATATGTAAACCACCAAAC+TGG + chr2_1:34811607-34811626 None:intergenic 30.0%
! TGGTGGTTTACATATTTTCT+AGG - chr2_1:34811610-34811629 Msa0206130:five_prime_UTR 30.0%
ATTGCCTAGTATAAAAGAGA+TGG - chr2_1:34811809-34811828 Msa0206130:intron 30.0%
! TTCTCCATCTCTTTTATACT+AGG + chr2_1:34811816-34811835 None:intergenic 30.0%
TAGTATAAAAGAGATGGAGA+AGG - chr2_1:34811815-34811834 Msa0206130:intron 30.0%
TGGAGAAGGATGTTAAATTA+TGG - chr2_1:34811829-34811848 Msa0206130:intron 30.0%
GGAGAAGGATGTTAAATTAT+GGG - chr2_1:34811830-34811849 Msa0206130:intron 30.0%
ACAAGACCATTATAATCAGT+GGG + chr2_1:34812008-34812027 None:intergenic 30.0%
AACAAGACCATTATAATCAG+TGG + chr2_1:34812009-34812028 None:intergenic 30.0%
ATAAGCTGTTTATCCAAACG+GGG - chr2_1:34809603-34809622 Msa0206130:CDS 35.0%
CTTTGCATTGGGAAATATAG+TGG - chr2_1:34809901-34809920 Msa0206130:intron 35.0%
TACCAATGGTGAAAGTAAAG+TGG + chr2_1:34810412-34810431 None:intergenic 35.0%
CTTGGTCAAAAAAGGAATCA+GGG + chr2_1:34810729-34810748 None:intergenic 35.0%
ACTTGGTCAAAAAAGGAATC+AGG + chr2_1:34810730-34810749 None:intergenic 35.0%
ATTCTTGCCACAGGATATAA+AGG - chr2_1:34810853-34810872 Msa0206130:CDS 35.0%
TTGCTAAATGACACCTTCAT+GGG + chr2_1:34811126-34811145 None:intergenic 35.0%
ATTGCTAAATGACACCTTCA+TGG + chr2_1:34811127-34811146 None:intergenic 35.0%
! CTAGAAGACACCTTTTATGA+AGG + chr2_1:34811203-34811222 None:intergenic 35.0%
! GGTGGTTTACTCAAGTATTT+TGG + chr2_1:34811260-34811279 None:intergenic 35.0%
CTAAATTAAGTCTCTCGTCA+TGG + chr2_1:34811507-34811526 None:intergenic 35.0%
TTTCTTGTAGTGGTTTACCA+TGG + chr2_1:34811671-34811690 None:intergenic 35.0%
GGATGTAGTATTTCTTGTAG+TGG + chr2_1:34811681-34811700 None:intergenic 35.0%
ATCCTATCATTGCCAATTGT+GGG + chr2_1:34811710-34811729 None:intergenic 35.0%
TATCCTATCATTGCCAATTG+TGG + chr2_1:34811711-34811730 None:intergenic 35.0%
CTAATGAAATGAGATGCCAA+TGG - chr2_1:34811757-34811776 Msa0206130:five_prime_UTR 35.0%
CTAGAAAATGTGCTAACCAT+TGG + chr2_1:34811776-34811795 None:intergenic 35.0%
! TGGTTAGCACATTTTCTAGA+TGG - chr2_1:34811777-34811796 Msa0206130:five_prime_UTR 35.0%
! AGATCATGCATTGTTACTTG+TGG - chr2_1:34811891-34811910 Msa0206130:five_prime_UTR 35.0%
GAAGTTGAGTTTGCACTCTT+AGG + chr2_1:34809629-34809648 None:intergenic 40.0%
! AAAGGCCCAGAAGTTTTTAC+AGG - chr2_1:34809955-34809974 Msa0206130:intron 40.0%
! TTCTATGGATTACTCTGCCT+TGG - chr2_1:34809990-34810009 Msa0206130:intron 40.0%
ACCATTTGCATTTGCGCATT+CGG + chr2_1:34810097-34810116 None:intergenic 40.0%
TCTCCCAGATTTCAACATTC+TGG - chr2_1:34810299-34810318 Msa0206130:intron 40.0%
CTCCCAGATTTCAACATTCT+GGG - chr2_1:34810300-34810319 Msa0206130:intron 40.0%
TGCCCAGAATGTTGAAATCT+GGG + chr2_1:34810305-34810324 None:intergenic 40.0%
ATGCCCAGAATGTTGAAATC+TGG + chr2_1:34810306-34810325 None:intergenic 40.0%
!!! GAACTTTTAGTTCACAAGCC+CGG - chr2_1:34810360-34810379 Msa0206130:intron 40.0%
GTGAAAGTAAAGTGGCCAAA+AGG + chr2_1:34810404-34810423 None:intergenic 40.0%
AAGTTGCCACTGAAGAAGTA+TGG - chr2_1:34810646-34810665 Msa0206130:CDS 40.0%
ACTAGTCCATACTTCTTCAG+TGG + chr2_1:34810655-34810674 None:intergenic 40.0%
! CGATGGATCCTTCTTTTACT+TGG + chr2_1:34810747-34810766 None:intergenic 40.0%
! AGCTTTAGCTTCTGCAAAGA+AGG - chr2_1:34810784-34810803 Msa0206130:CDS 40.0%
GATGTAGTTATTCTTGCCAC+AGG - chr2_1:34810844-34810863 Msa0206130:CDS 40.0%
TTGGTCACCTTTATATCCTG+TGG + chr2_1:34810863-34810882 None:intergenic 40.0%
GTTCAAGGATTTCTCTCTAC+AGG - chr2_1:34810932-34810951 Msa0206130:CDS 40.0%
ATTTAGCAATCCACACCTGA+AGG - chr2_1:34811137-34811156 Msa0206130:CDS 40.0%
AAAGTGGAAATCACACCTTC+AGG + chr2_1:34811155-34811174 None:intergenic 40.0%
TACAAAGGCAGGGATCAAAT+TGG + chr2_1:34811281-34811300 None:intergenic 40.0%
TATGTTTGTGTCACATGACG+AGG - chr2_1:34811389-34811408 Msa0206130:intron 40.0%
ATCCCACAATTGGCAATGAT+AGG - chr2_1:34811705-34811724 Msa0206130:five_prime_UTR 40.0%
GCAATGATAGGATATGCTGA+GGG - chr2_1:34811717-34811736 Msa0206130:five_prime_UTR 40.0%
! GCATTGTTACTTGTGGCATA+TGG - chr2_1:34811898-34811917 Msa0206130:five_prime_UTR 40.0%
TCGGATTACACTTCATGTCT+CGG + chr2_1:34811941-34811960 None:intergenic 40.0%
TGTAATCCGAGGAGAAAGAA+GGG - chr2_1:34811951-34811970 Msa0206130:five_prime_UTR 40.0%
AAAAGACCCTTCTTTCTCCT+CGG + chr2_1:34811960-34811979 None:intergenic 40.0%
! GAGAAAGAAGGGTCTTTTTG+CGG - chr2_1:34811962-34811981 Msa0206130:five_prime_UTR 40.0%
CGGAGCTATTTGAACCATAT+GGG - chr2_1:34811982-34812001 Msa0206130:five_prime_UTR 40.0%
TTATAATCAGTGGGCCCATA+TGG + chr2_1:34811999-34812018 None:intergenic 40.0%
! TATGGGCCCACTGATTATAA+TGG - chr2_1:34811999-34812018 Msa0206130:five_prime_UTR 40.0%
! AAACTTCTGGGCCTTTTCCA+GGG + chr2_1:34809951-34809970 None:intergenic 45.0%
! AAAACTTCTGGGCCTTTTCC+AGG + chr2_1:34809952-34809971 None:intergenic 45.0%
AGCAGCAGTTTCTTTGTCCA+AGG + chr2_1:34810010-34810029 None:intergenic 45.0%
AGAGTTACAGTAGTAGGCTC+AGG - chr2_1:34810048-34810067 Msa0206130:intron 45.0%
TCCGAATGCGCAAATGCAAA+TGG - chr2_1:34810093-34810112 Msa0206130:intron 45.0%
AACATTCTGGGCATTCGTCT+CGG - chr2_1:34810312-34810331 Msa0206130:intron 45.0%
!! ACACTTCCTTCTTGGCCTTT+TGG - chr2_1:34810386-34810405 Msa0206130:intron 45.0%
! GGCCACTTTACTTTCACCAT+TGG - chr2_1:34810407-34810426 Msa0206130:intron 45.0%
CTAAATGACACCTTCATGGG+TGG + chr2_1:34811123-34811142 None:intergenic 45.0%
AAAGGCAGGGATCAAATTGG+TGG + chr2_1:34811278-34811297 None:intergenic 45.0%
GAGAGTGACTTACAAAGGCA+GGG + chr2_1:34811291-34811310 None:intergenic 45.0%
TGAGAGTGACTTACAAAGGC+AGG + chr2_1:34811292-34811311 None:intergenic 45.0%
TGCAAAGACAGAGACCAGTT+TGG - chr2_1:34811590-34811609 Msa0206130:five_prime_UTR 45.0%
AAAGACAGAGACCAGTTTGG+TGG - chr2_1:34811593-34811612 Msa0206130:five_prime_UTR 45.0%
GGCAATGATAGGATATGCTG+AGG - chr2_1:34811716-34811735 Msa0206130:five_prime_UTR 45.0%
GAGACATGAAGTGTAATCCG+AGG - chr2_1:34811940-34811959 Msa0206130:five_prime_UTR 45.0%
GTGTAATCCGAGGAGAAAGA+AGG - chr2_1:34811950-34811969 Msa0206130:five_prime_UTR 45.0%
GCGGAGCTATTTGAACCATA+TGG - chr2_1:34811981-34812000 Msa0206130:five_prime_UTR 45.0%
GGGAGGAACTCAGGAATGTT+TGG + chr2_1:34809931-34809950 None:intergenic 50.0%
AACATTCCTGAGTTCCTCCC+TGG - chr2_1:34809931-34809950 Msa0206130:intron 50.0%
!! CCAAGAAGGAAGTGTTCTCC+GGG + chr2_1:34810381-34810400 None:intergenic 50.0%
!! GCCAAGAAGGAAGTGTTCTC+CGG + chr2_1:34810382-34810401 None:intergenic 50.0%
AAGTGGCCAAAAGGCCAAGA+AGG + chr2_1:34810395-34810414 None:intergenic 50.0%
TTGCTAGAAGCCACCCATGA+AGG - chr2_1:34811110-34811129 Msa0206130:intron 50.0%
GGAAATCACACCTTCAGGTG+TGG + chr2_1:34811150-34811169 None:intergenic 50.0%
ACATCCTCGCATCCCACAAT+TGG - chr2_1:34811695-34811714 Msa0206130:five_prime_UTR 50.0%
ATTGCCAATTGTGGGATGCG+AGG + chr2_1:34811702-34811721 None:intergenic 50.0%
TTGCACTCTTAGGCCCCGTT+TGG + chr2_1:34809619-34809638 None:intergenic 55.0%
! CCTTTTCCAGGGAGGAACTC+AGG + chr2_1:34809940-34809959 None:intergenic 55.0%
CCTGAGTTCCTCCCTGGAAA+AGG - chr2_1:34809937-34809956 Msa0206130:intron 55.0%
CCCGGAGAACACTTCCTTCT+TGG - chr2_1:34810378-34810397 Msa0206130:intron 55.0%
! CTTCTGGGCCTTTTCCAGGG+AGG + chr2_1:34809948-34809967 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 34809528 34812037 34809528 ID=Msa0206130;Name=Msa0206130
chr2_1 mRNA 34809528 34812037 34809528 ID=Msa0206130-mRNA-1;Parent=Msa0206130;Name=Msa0206130-mRNA-1;_AED=0.15;_eAED=0.15;_QI=429|0.6|0.5|1|0.4|0.33|6|0|316
chr2_1 exon 34809528 34809838 34809528 ID=Msa0206130-mRNA-1:exon:11617;Parent=Msa0206130-mRNA-1
chr2_1 exon 34810612 34810699 34810612 ID=Msa0206130-mRNA-1:exon:11616;Parent=Msa0206130-mRNA-1
chr2_1 exon 34810716 34810961 34810716 ID=Msa0206130-mRNA-1:exon:11615;Parent=Msa0206130-mRNA-1
chr2_1 exon 34811137 34811312 34811137 ID=Msa0206130-mRNA-1:exon:11614;Parent=Msa0206130-mRNA-1
chr2_1 exon 34811451 34811810 34811451 ID=Msa0206130-mRNA-1:exon:11613;Parent=Msa0206130-mRNA-1
chr2_1 exon 34811839 34812037 34811839 ID=Msa0206130-mRNA-1:exon:11612;Parent=Msa0206130-mRNA-1
chr2_1 five_prime_UTR 34811839 34812037 34811839 ID=Msa0206130-mRNA-1:five_prime_utr;Parent=Msa0206130-mRNA-1
chr2_1 five_prime_UTR 34811581 34811810 34811581 ID=Msa0206130-mRNA-1:five_prime_utr;Parent=Msa0206130-mRNA-1
chr2_1 CDS 34811451 34811580 34811451 ID=Msa0206130-mRNA-1:cds;Parent=Msa0206130-mRNA-1
chr2_1 CDS 34811137 34811312 34811137 ID=Msa0206130-mRNA-1:cds;Parent=Msa0206130-mRNA-1
chr2_1 CDS 34810716 34810961 34810716 ID=Msa0206130-mRNA-1:cds;Parent=Msa0206130-mRNA-1
chr2_1 CDS 34810612 34810699 34810612 ID=Msa0206130-mRNA-1:cds;Parent=Msa0206130-mRNA-1
chr2_1 CDS 34809528 34809838 34809528 ID=Msa0206130-mRNA-1:cds;Parent=Msa0206130-mRNA-1
Gene Sequence

>Msa0206130

ATGCATTCTATGGATTACTCTGCCTTGGACAAAGAAACTGCTGCTAAATTGATAAAAAATAAGAGAGTTACAGTAGTAGGCTCAGGAAAATCTGCTCTTGATATAGCTTCCGAATGCGCAAATGCAAATGGAATCACTCACCCTTGCACGATTATAAATAGAACTTCACACTGGTTTCTCCCAGATTTCAACATTCTGGGCATTCGTCTCGGATACTTGTATTTCAATCGTTTCGCAGAACTTTTAGTTCACAAGCCCGGAGAACACTTCCTTCTTGGCCTTTTGGCCACTTTACTTTCACCATTGAGATGGGGAATTTCTAAACTTGTTGAAACATATCTGAAATGGAAGTTGCCACTGAAGAAGTATGGACTAGTACCTAATCATAGTTTTCTTCAAGAAATATCTGCATGTCAAATTGGAATTCTCCCTGATTCCTTTTTTGACCAAGTAAAAGAAGGATCCATCGAGATAAAGAAATCACAAAGCTTTAGCTTCTGCAAAGAAGGTTTGATCATCAATGAAGAAGCTAAGCCCTTAGAAACAGATGTAGATATAAAGGTGACCAAAAACTTAAAAGCATATTCAAATCTCCAATATTCCAAAATTACATTGACAGTTCAAGGATTTCTCTCTACAGGATATGCTGAGGGAATATCAAATATTGTTTCTAATGAAATGAGATGCCAATGGTTAGCACATTTTCTAGATGGAAACATTGAATTGCCTAGTATAAAAGAGATGGAGAAGGATGTTAAATTATGGGAAGACAACATGAAGCAATATTCCAGAAAATTTTATTGGAGATCATGCATTGTTACTTGTGGCATATGGTATAATGATCAATTGTGCCGAGACATGAAGTGTAATCCGAGGAGAAAGAAGGGTCTTTTTGCGGAGCTATTTGAACCATATGGGCCCACTGATTATAATGGTCTTGTTCGTAAATGA
Protein sequence

>Msa0206130

MHSMDYSALDKETAAKLIKNKRVTVVGSGKSALDIASECANANGITHPCTIINRTSHWFLPDFNILGIRLGYLYFNRFAELLVHKPGEHFLLGLLATLLSPLRWGISKLVETYLKWKLPLKKYGLVPNHSFLQEISACQIGILPDSFFDQVKEGSIEIKKSQSFSFCKEGLIINEEAKPLETDVDIKVTKNLKAYSNLQYSKITLTVQGFLSTGYAEGISNIVSNEMRCQWLAHFLDGNIELPSIKEMEKDVKLWEDNMKQYSRKFYWRSCIVTCGIWYNDQLCRDMKCNPRRKKGLFAELFEPYGPTDYNGLVRK*