AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0216830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0216830 MtrunA17_Chr2g0318421 97.087 103 3 0 1 103 1 103 5.34e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAATTCAGTTATTTGTTT+AGG 0.384801 2_1:-63010435 Msa0216830:three_prime_UTR
CGCGGCAGACTTCTCATTTG+CGG 0.410034 2_1:+63010612 None:intergenic
TCTACGAATAAATAACTTGC+TGG 0.413180 2_1:-63010537 Msa0216830:exon
GGAGCTAAAATCAAGGTTGC+TGG 0.433275 2_1:-63012592 Msa0216830:CDS
GCAATGGATACATCAGAATC+AGG 0.468029 2_1:-63012613 Msa0216830:CDS
GAATATACCTTGAGGTGCTT+CGG 0.481198 2_1:+63012783 None:intergenic
CGGTGAAGCGGCGCAGAGAA+AGG 0.493829 2_1:+63012896 None:intergenic
CTTCAATCTTAAATAACACT+TGG 0.499964 2_1:+63010412 None:intergenic
GTCGGTTATGTCCATCGAAA+CGG 0.525995 2_1:+63012816 None:intergenic
CACCGTCCGCTCCAATCCAA+TGG 0.550931 2_1:-63012878 Msa0216830:exon
AACAGGAAGGTGAAGCAGAA+TGG 0.552894 2_1:-63010694 Msa0216830:intron
CGCCATTGGATTGGAGCGGA+CGG 0.556534 2_1:+63012876 None:intergenic
TTCAGACTTGGAAACTTTCG+CGG 0.568580 2_1:+63010594 None:intergenic
CGAATAAATAACTTGCTGGA+TGG 0.589204 2_1:-63010533 Msa0216830:exon
GATTGGAGCGGACGGTGAAG+CGG 0.593944 2_1:+63012884 None:intergenic
AGAATCAGGAGCTAAAATCA+AGG 0.594733 2_1:-63012599 Msa0216830:CDS
TCGTCTAAACTAAATTACAA+AGG 0.595938 2_1:+63010454 None:intergenic
GGAAGGTGAAGCAGAATGGA+AGG 0.604858 2_1:-63010690 Msa0216830:CDS
ATCGTTTCCGAAGCACCTCA+AGG 0.606213 2_1:-63012790 Msa0216830:intron
AAGTATCAAAATTTAATGAG+TGG 0.607047 2_1:+63010501 None:intergenic
GCTAAAATCAAGGTTGCTGG+TGG 0.607057 2_1:-63012589 Msa0216830:CDS
TGCTTCGGAAACGATGGTGT+CGG 0.636322 2_1:+63012798 None:intergenic
TTGAGGTGCTTCGGAAACGA+TGG 0.645225 2_1:+63012792 None:intergenic
GTAAACCTAAAACAGAGCTG+TGG 0.697872 2_1:+63010310 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTATAATTTTTTAAAGA+AGG - chr2_1:63011579-63011598 Msa0216830:intron 10.0%
!!! TTCCTTTTTTTTTTTTTTTG+TGG - chr2_1:63010622-63010641 Msa0216830:CDS 15.0%
!! AACCACAAAAAAAAAAAAAA+AGG + chr2_1:63010627-63010646 None:intergenic 15.0%
!! AATAAAGAAAACTTACAAAA+TGG + chr2_1:63010731-63010750 None:intergenic 15.0%
!!! TTATAATTTTTTAAAGAAGG+CGG - chr2_1:63011582-63011601 Msa0216830:intron 15.0%
!! AAAAACAAGAAGAACAAAAA+CGG - chr2_1:63010448-63010467 Msa0216830:three_prime_UTR 20.0%
!!! TTAATTTGTTTATCCAAACA+AGG + chr2_1:63010925-63010944 None:intergenic 20.0%
!! AATCAAACTTATTGGATTAA+GGG + chr2_1:63011063-63011082 None:intergenic 20.0%
!! AAATCAAACTTATTGGATTA+AGG + chr2_1:63011064-63011083 None:intergenic 20.0%
!! TTAATCCAATAAGTTTGATT+TGG - chr2_1:63011063-63011082 Msa0216830:intron 20.0%
!! TTTGCATAATAATAGTTGTA+TGG - chr2_1:63012255-63012274 Msa0216830:intron 20.0%
!! TTGCATAATAATAGTTGTAT+GGG - chr2_1:63012256-63012275 Msa0216830:intron 20.0%
!!! CATAAAGTTTTAAACAATGA+TGG + chr2_1:63012409-63012428 None:intergenic 20.0%
!!! CATCATTGTTTAAAACTTTA+TGG - chr2_1:63012407-63012426 Msa0216830:intron 20.0%
!! AAGTATCAAAATTTAATGAG+TGG + chr2_1:63012791-63012810 None:intergenic 20.0%
!! ATAAAGATGCAACAAAAATA+AGG + chr2_1:63012905-63012924 None:intergenic 20.0%
! TAGAGAATCACAAGTTAAAT+GGG - chr2_1:63010797-63010816 Msa0216830:intron 25.0%
!! ACCAAAGCATTAAAAAACTA+GGG + chr2_1:63010853-63010872 None:intergenic 25.0%
!! AACCAAAGCATTAAAAAACT+AGG + chr2_1:63010854-63010873 None:intergenic 25.0%
!!! TTTTTTAATGCTTTGGTTGA+TGG - chr2_1:63010856-63010875 Msa0216830:intron 25.0%
! AAATACCAACTTAGAATATG+AGG + chr2_1:63011172-63011191 None:intergenic 25.0%
!! TTTAAAACATATATGTGCGT+AGG + chr2_1:63011617-63011636 None:intergenic 25.0%
!!! AAAAAGTGTCATCAAAATGA+GGG + chr2_1:63011905-63011924 None:intergenic 25.0%
!!! AAAAAAGTGTCATCAAAATG+AGG + chr2_1:63011906-63011925 None:intergenic 25.0%
! ACAGTTAACATATAACTCAT+AGG + chr2_1:63011983-63012002 None:intergenic 25.0%
! TTCTATCAAACAATCATTCA+AGG + chr2_1:63012068-63012087 None:intergenic 25.0%
! AATGATTGTTTGATAGAACA+AGG - chr2_1:63012070-63012089 Msa0216830:intron 25.0%
! AGTCTATCAATAGGAAAATT+TGG - chr2_1:63012220-63012239 Msa0216830:intron 25.0%
!! TATTATGTTTTGCAAGTAGA+TGG + chr2_1:63012482-63012501 None:intergenic 25.0%
!!! ACTTTTTTTGTTTTCAGACT+TGG + chr2_1:63012710-63012729 None:intergenic 25.0%
! TCGTCTAAACTAAATTACAA+AGG + chr2_1:63012838-63012857 None:intergenic 25.0%
! ACGAATTCAGTTATTTGTTT+AGG - chr2_1:63012854-63012873 Msa0216830:CDS 25.0%
! CTTCAATCTTAAATAACACT+TGG + chr2_1:63012880-63012899 None:intergenic 25.0%
!! TTTTCTCAAATATCGACTGA+TGG + chr2_1:63010351-63010370 None:intergenic 30.0%
!! CTTTTGTTTATTTGCAGCAA+TGG - chr2_1:63010660-63010679 Msa0216830:CDS 30.0%
GACGAAGGCTAAAAATATAA+GGG + chr2_1:63010755-63010774 None:intergenic 30.0%
GTAGAGAATCACAAGTTAAA+TGG - chr2_1:63010796-63010815 Msa0216830:intron 30.0%
AGAGAATCACAAGTTAAATG+GGG - chr2_1:63010798-63010817 Msa0216830:intron 30.0%
!!! ACCCTAGTTTTTTAATGCTT+TGG - chr2_1:63010849-63010868 Msa0216830:intron 30.0%
CTTATTCATAACCACTCATA+TGG + chr2_1:63010961-63010980 None:intergenic 30.0%
TTGCACCAAATCAAACTTAT+TGG + chr2_1:63011071-63011090 None:intergenic 30.0%
! ATTGGATGTTGTTGTTGAAT+TGG - chr2_1:63011121-63011140 Msa0216830:intron 30.0%
! TTGGATGTTGTTGTTGAATT+GGG - chr2_1:63011122-63011141 Msa0216830:intron 30.0%
ACTTAGAATATGAGGAGATA+TGG + chr2_1:63011164-63011183 None:intergenic 30.0%
TATCTCCTCATATTCTAAGT+TGG - chr2_1:63011164-63011183 Msa0216830:intron 30.0%
ATCACTTAACTGTGTGATAT+AGG - chr2_1:63011249-63011268 Msa0216830:intron 30.0%
!!! GCAATGTAGTTTTTTTTGCA+TGG - chr2_1:63011348-63011367 Msa0216830:intron 30.0%
!! AATCACATTTACTTTTGCAC+TGG - chr2_1:63011390-63011409 Msa0216830:intron 30.0%
!! TATTCTTTAAAGCATGCACT+TGG - chr2_1:63011470-63011489 Msa0216830:intron 30.0%
! ATAGTAGTTGCCTTGTATTT+TGG - chr2_1:63011520-63011539 Msa0216830:intron 30.0%
! TAGTAGTTGCCTTGTATTTT+GGG - chr2_1:63011521-63011540 Msa0216830:intron 30.0%
TGTTCGTATTCTAATTACAC+AGG + chr2_1:63011752-63011771 None:intergenic 30.0%
GAATACGAACAATTCACTAA+TGG - chr2_1:63011761-63011780 Msa0216830:intron 30.0%
CTTACAAAATTGATCAGCAT+TGG + chr2_1:63011796-63011815 None:intergenic 30.0%
CATGTTATAATTGAGGACAT+TGG + chr2_1:63012015-63012034 None:intergenic 30.0%
ATTCAAGGCAAAAATTCTGA+TGG + chr2_1:63012053-63012072 None:intergenic 30.0%
ATCATAGACTCGATTGATAT+CGG + chr2_1:63012100-63012119 None:intergenic 30.0%
ATATCAATCGAGTCTATGAT+AGG - chr2_1:63012100-63012119 Msa0216830:intron 30.0%
AGCCAAATAGAGAGTTAAAT+AGG - chr2_1:63012127-63012146 Msa0216830:intron 30.0%
GCCAAATAGAGAGTTAAATA+GGG - chr2_1:63012128-63012147 Msa0216830:intron 30.0%
CCAAATAGAGAGTTAAATAG+GGG - chr2_1:63012129-63012148 Msa0216830:intron 30.0%
ATAACTGACAATAGTTAGCT+AGG - chr2_1:63012180-63012199 Msa0216830:intron 30.0%
!! TGTATGGGATATTTGTTATC+AGG - chr2_1:63012271-63012290 Msa0216830:intron 30.0%
AAAACTGATGAACAATTCAC+AGG + chr2_1:63012309-63012328 None:intergenic 30.0%
AATATTCTATACTTTCGCAG+AGG - chr2_1:63012334-63012353 Msa0216830:intron 30.0%
TCACGAAGTATATTACAGAA+GGG + chr2_1:63012366-63012385 None:intergenic 30.0%
!! ACACTTTTTGATCTCTTCAT+GGG + chr2_1:63012625-63012644 None:intergenic 30.0%
!! AACACTTTTTGATCTCTTCA+TGG + chr2_1:63012626-63012645 None:intergenic 30.0%
! TCTACGAATAAATAACTTGC+TGG - chr2_1:63012752-63012771 Msa0216830:intron 30.0%
TGAAAAGCGAATATACCTTG+AGG + chr2_1:63010517-63010536 None:intergenic 35.0%
AGAATCAGGAGCTAAAATCA+AGG - chr2_1:63010690-63010709 Msa0216830:CDS 35.0%
CGACGAAGGCTAAAAATATA+AGG + chr2_1:63010756-63010775 None:intergenic 35.0%
AAGTTAAATGGGGTTTAACG+CGG - chr2_1:63010808-63010827 Msa0216830:intron 35.0%
GCAACGATTTGAGAAAATGT+TGG + chr2_1:63010894-63010913 None:intergenic 35.0%
TTCTCAAATCGTTGCAATTC+AGG - chr2_1:63010899-63010918 Msa0216830:intron 35.0%
AATCTCACATGTGAATATGC+TGG - chr2_1:63010995-63011014 Msa0216830:intron 35.0%
TAATCAGTTGAGTAACTGGA+CGG + chr2_1:63011102-63011121 None:intergenic 35.0%
CCAATAATCAGTTGAGTAAC+TGG + chr2_1:63011106-63011125 None:intergenic 35.0%
CCAGTTACTCAACTGATTAT+TGG - chr2_1:63011103-63011122 Msa0216830:intron 35.0%
AGTCATCATTCTTTGTCGAA+CGG - chr2_1:63011221-63011240 Msa0216830:intron 35.0%
ACTGTGTGATATAGGAAATC+TGG - chr2_1:63011257-63011276 Msa0216830:intron 35.0%
CTGTGTGATATAGGAAATCT+GGG - chr2_1:63011258-63011277 Msa0216830:intron 35.0%
AGTTGAACGCAATTATAGGA+GGG + chr2_1:63011291-63011310 None:intergenic 35.0%
GTACAGTTGAACGCAATTAT+AGG + chr2_1:63011295-63011314 None:intergenic 35.0%
CCATTGCTACAATGAAGAAA+TGG + chr2_1:63011329-63011348 None:intergenic 35.0%
CCATTTCTTCATTGTAGCAA+TGG - chr2_1:63011326-63011345 Msa0216830:intron 35.0%
!! TTACTTTTGCACTGGATGTT+AGG - chr2_1:63011398-63011417 Msa0216830:intron 35.0%
ATCTGTTGAGTTCTTGAAGA+AGG - chr2_1:63011446-63011465 Msa0216830:intron 35.0%
TCTGTTGAGTTCTTGAAGAA+GGG - chr2_1:63011447-63011466 Msa0216830:intron 35.0%
CAAAGCAAACCCAAAATACA+AGG + chr2_1:63011533-63011552 None:intergenic 35.0%
! ATAATGTTTTCTGCTCTGCT+GGG + chr2_1:63011711-63011730 None:intergenic 35.0%
TTACACAGGCAATTTAGTGA+TGG + chr2_1:63011738-63011757 None:intergenic 35.0%
! TTTGCTCTTCTGTTTTGTCA+TGG - chr2_1:63011952-63011971 Msa0216830:intron 35.0%
! TTGCTCTTCTGTTTTGTCAT+GGG - chr2_1:63011953-63011972 Msa0216830:intron 35.0%
TCATAGACTCGATTGATATC+GGG + chr2_1:63012099-63012118 None:intergenic 35.0%
CCCCTATTTAACTCTCTATT+TGG + chr2_1:63012132-63012151 None:intergenic 35.0%
CTCACGAAGTATATTACAGA+AGG + chr2_1:63012367-63012386 None:intergenic 35.0%
TTTGCAAGTAGATGGAAATC+AGG + chr2_1:63012474-63012493 None:intergenic 35.0%
!! CGAATAAATAACTTGCTGGA+TGG - chr2_1:63012756-63012775 Msa0216830:intron 35.0%
! AAAAACCACAGCTCTGTTTT+AGG - chr2_1:63012974-63012993 Msa0216830:five_prime_UTR 35.0%
!! TTGTTCCTGTTTTTCGCCAT+TGG + chr2_1:63010430-63010449 None:intergenic 40.0%
! GAATATACCTTGAGGTGCTT+CGG + chr2_1:63010509-63010528 None:intergenic 40.0%
GCAATGGATACATCAGAATC+AGG - chr2_1:63010676-63010695 Msa0216830:CDS 40.0%
TCACATGTGAATATGCTGGT+AGG - chr2_1:63010999-63011018 Msa0216830:intron 40.0%
CAGTTGAACGCAATTATAGG+AGG + chr2_1:63011292-63011311 None:intergenic 40.0%
!! TGGATGTTAGGTTCTAGACT+TGG - chr2_1:63011410-63011429 Msa0216830:intron 40.0%
! GATAATGTTTTCTGCTCTGC+TGG + chr2_1:63011712-63011731 None:intergenic 40.0%
AGTTCAGTTCGCATAGTTGA+TGG - chr2_1:63011851-63011870 Msa0216830:intron 40.0%
TCCTCAATTATAACATGCGC+TGG - chr2_1:63012018-63012037 Msa0216830:intron 40.0%
TCCAGCGCATGTTATAATTG+AGG + chr2_1:63012022-63012041 None:intergenic 40.0%
AGTTAGCTAGGAAGAGTAGA+TGG - chr2_1:63012192-63012211 Msa0216830:intron 40.0%
ATGGATGGGAGTCTATCAAT+AGG - chr2_1:63012211-63012230 Msa0216830:intron 40.0%
ACTGATGAACAATTCACAGG+AGG + chr2_1:63012306-63012325 None:intergenic 40.0%
GTGTTTACTCCACGACATAT+TGG + chr2_1:63012438-63012457 None:intergenic 40.0%
TTCAGACTTGGAAACTTTCG+CGG + chr2_1:63012698-63012717 None:intergenic 40.0%
GTAAACCTAAAACAGAGCTG+TGG + chr2_1:63012982-63013001 None:intergenic 40.0%
!! TTTTCGCCATTGGATTGGAG+CGG + chr2_1:63010420-63010439 None:intergenic 45.0%
!! CCTGTTTTTCGCCATTGGAT+TGG + chr2_1:63010425-63010444 None:intergenic 45.0%
CCAATCCAATGGCGAAAAAC+AGG - chr2_1:63010422-63010441 Msa0216830:three_prime_UTR 45.0%
GTCGGTTATGTCCATCGAAA+CGG + chr2_1:63010476-63010495 None:intergenic 45.0%
GGAGCTAAAATCAAGGTTGC+TGG - chr2_1:63010697-63010716 Msa0216830:intron 45.0%
GCTAAAATCAAGGTTGCTGG+TGG - chr2_1:63010700-63010719 Msa0216830:intron 45.0%
! ATTATCAAGCATCGCGACGA+AGG + chr2_1:63010770-63010789 None:intergenic 45.0%
!! GTTGCAATTCAGGCCTTGTT+TGG - chr2_1:63010909-63010928 Msa0216830:intron 45.0%
TGCAGCTTCTACCATATGAG+TGG - chr2_1:63010947-63010966 Msa0216830:intron 45.0%
CGCAATTATAGGAGGGAAGA+GGG + chr2_1:63011284-63011303 None:intergenic 45.0%
ACGCAATTATAGGAGGGAAG+AGG + chr2_1:63011285-63011304 None:intergenic 45.0%
AGCTAGGAAGAGTAGATGGA+TGG - chr2_1:63012196-63012215 Msa0216830:intron 45.0%
GCTAGGAAGAGTAGATGGAT+GGG - chr2_1:63012197-63012216 Msa0216830:intron 45.0%
AACAATTCACAGGAGGTTCG+AGG + chr2_1:63012299-63012318 None:intergenic 45.0%
GTAGATGGAAATCAGGAAGC+AGG + chr2_1:63012467-63012486 None:intergenic 45.0%
!!! GCATGCTTTTGCTCTGAAAC+AGG - chr2_1:63012578-63012597 Msa0216830:CDS 45.0%
!!! GCTTTTGCTCTGAAACAGGA+AGG - chr2_1:63012582-63012601 Msa0216830:CDS 45.0%
AACAGGAAGGTGAAGCAGAA+TGG - chr2_1:63012595-63012614 Msa0216830:CDS 45.0%
!! CAAAAACGGTGCCGTTTCGA+TGG - chr2_1:63010462-63010481 Msa0216830:three_prime_UTR 50.0%
TGCTTCGGAAACGATGGTGT+CGG + chr2_1:63010494-63010513 None:intergenic 50.0%
! TTGAGGTGCTTCGGAAACGA+TGG + chr2_1:63010500-63010519 None:intergenic 50.0%
! ATCGTTTCCGAAGCACCTCA+AGG - chr2_1:63010499-63010518 Msa0216830:three_prime_UTR 50.0%
!! ACTTGGTGCACTGCAAGATG+TGG - chr2_1:63011487-63011506 Msa0216830:intron 50.0%
ATGGTGCGTCCAATATGTCG+TGG - chr2_1:63012426-63012445 Msa0216830:intron 50.0%
GGAAGGTGAAGCAGAATGGA+AGG - chr2_1:63012599-63012618 Msa0216830:CDS 50.0%
CGCGGCAGACTTCTCATTTG+CGG + chr2_1:63012680-63012699 None:intergenic 55.0%
GATTGGAGCGGACGGTGAAG+CGG + chr2_1:63010408-63010427 None:intergenic 60.0%
CACCGTCCGCTCCAATCCAA+TGG - chr2_1:63010411-63010430 Msa0216830:three_prime_UTR 60.0%
! CGCCATTGGATTGGAGCGGA+CGG + chr2_1:63010416-63010435 None:intergenic 60.0%
CGGTGAAGCGGCGCAGAGAA+AGG + chr2_1:63010396-63010415 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 63010309 63013002 63010309 ID=Msa0216830;Name=Msa0216830
chr2_1 mRNA 63010309 63013002 63010309 ID=Msa0216830-mRNA-1;Parent=Msa0216830;Name=Msa0216830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=121|1|1|1|1|1|3|240|103
chr2_1 exon 63010309 63010711 63010309 ID=Msa0216830-mRNA-1:exon:17505;Parent=Msa0216830-mRNA-1
chr2_1 exon 63012577 63012634 63012577 ID=Msa0216830-mRNA-1:exon:17504;Parent=Msa0216830-mRNA-1
chr2_1 exon 63012791 63013002 63012791 ID=Msa0216830-mRNA-1:exon:17503;Parent=Msa0216830-mRNA-1
chr2_1 five_prime_UTR 63012882 63013002 63012882 ID=Msa0216830-mRNA-1:five_prime_utr;Parent=Msa0216830-mRNA-1
chr2_1 CDS 63012791 63012881 63012791 ID=Msa0216830-mRNA-1:cds;Parent=Msa0216830-mRNA-1
chr2_1 CDS 63012577 63012634 63012577 ID=Msa0216830-mRNA-1:cds;Parent=Msa0216830-mRNA-1
chr2_1 CDS 63010549 63010711 63010549 ID=Msa0216830-mRNA-1:cds;Parent=Msa0216830-mRNA-1
chr2_1 three_prime_UTR 63010309 63010548 63010309 ID=Msa0216830-mRNA-1:three_prime_utr;Parent=Msa0216830-mRNA-1
Gene Sequence

>Msa0216830

ATGGCGAAAAACAGGAACAAAAACAAGAAGAACAAAAACGGTGCCGTTTCGATGGACATAACCGACACCATCGTTTCCGAAGCACCTCAAGCAATGGATACATCAGAATCAGGAGCTAAAATCAAGGTTGCTGGTGGTGCTACCATTTTGAAGGTGAAGCAGAATGGAAGGCCCATGAAGAGATCAAAAAGTGTTCGAAAGATGAAGAAAATAGCAAAAGCTATATCCGCAAATGAGAAGTCTGCCGCGAAAGTTTCCAAGTCTGAAAACAAAAAAAGTAGAGTTCAATCTGCGAAAACACTCTACGAATAA

Protein sequence

>Msa0216830

MAKNRNKNKKNKNGAVSMDITDTIVSEAPQAMDTSESGAKIKVAGGATILKVKQNGRPMKRSKSVRKMKKIAKAISANEKSAAKVSKSENKKSRVQSAKTLYE*