AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0321650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321650 MtrunA17_Chr2g0298861 92.687 629 35 1 1 618 1 629 0.0 1186
Msa0321650 MtrunA17_Chr7g0223721 81.583 619 113 1 1 619 1 618 0.0 1034
Msa0321650 MtrunA17_Chr4g0040931 80.906 618 117 1 1 618 1 617 0.0 1015
Msa0321650 MtrunA17_Chr2g0298851 75.121 619 153 1 1 619 1 618 0.0 977
Msa0321650 MtrunA17_Chr7g0223851 73.258 617 164 1 1 617 10 625 0.0 935
Msa0321650 MtrunA17_Chr2g0293581 71.613 620 173 3 1 618 1 619 0.0 927
Msa0321650 MtrunA17_Chr5g0427901 71.815 628 164 3 1 617 1 626 0.0 920
Msa0321650 MtrunA17_Chr7g0225671 72.757 613 165 2 6 618 13 623 0.0 913
Msa0321650 MtrunA17_Chr7g0233311 71.729 619 173 2 1 618 1 618 0.0 911
Msa0321650 MtrunA17_Chr7g0221661 71.916 616 171 2 1 616 1 614 0.0 903
Msa0321650 MtrunA17_Chr7g0223801 71.870 615 169 4 1 615 1 611 0.0 903
Msa0321650 MtrunA17_Chr7g0223821 71.729 619 169 4 1 615 1 617 0.0 892
Msa0321650 MtrunA17_Chr7g0223821 47.222 108 46 2 519 615 596 703 4.16e-16 82.0
Msa0321650 MtrunA17_Chr4g0012541 69.854 617 176 2 1 617 1 607 0.0 882
Msa0321650 MtrunA17_Chr2g0287631 70.436 619 181 2 1 618 1 618 0.0 877
Msa0321650 MtrunA17_Chr7g0217971 70.315 603 152 4 1 603 1 576 0.0 858
Msa0321650 MtrunA17_Chr7g0223831 68.336 619 167 2 1 619 1 590 0.0 853
Msa0321650 MtrunA17_Chr7g0233301 68.013 619 165 2 1 618 1 587 0.0 850
Msa0321650 MtrunA17_Chr7g0215261 68.277 621 159 5 1 619 1 585 0.0 841
Msa0321650 MtrunA17_Chr7g0228281 67.528 619 153 6 1 618 62 633 0.0 830
Msa0321650 MtrunA17_Chr8g0390821 64.328 670 169 8 1 617 1 653 0.0 828
Msa0321650 MtrunA17_Chr7g0223181 65.105 619 210 5 1 617 1 615 0.0 810
Msa0321650 MtrunA17_Chr7g0223711 64.274 613 215 4 1 612 1 610 0.0 809
Msa0321650 MtrunA17_Chr7g0223781 64.878 615 212 3 1 615 1 611 0.0 806
Msa0321650 MtrunA17_Chr2g0296391 72.128 531 146 2 1 530 1 530 0.0 806
Msa0321650 MtrunA17_Chr8g0350731 65.139 611 210 3 1 611 29 636 0.0 805
Msa0321650 MtrunA17_Chr7g0223031 65.033 612 211 3 1 612 1 609 0.0 802
Msa0321650 MtrunA17_Chr4g0031301 64.459 619 214 5 1 617 1 615 0.0 802
Msa0321650 MtrunA17_Chr7g0223291 63.974 619 217 5 1 617 1 615 0.0 800
Msa0321650 MtrunA17_Chr7g0262821 64.136 619 216 5 1 617 1 615 0.0 798
Msa0321650 MtrunA17_Chr7g0223731 63.889 612 218 3 1 612 1 609 0.0 798
Msa0321650 MtrunA17_Chr4g0031321 64.297 619 215 5 1 617 1 615 0.0 797
Msa0321650 MtrunA17_Chr7g0223751 63.399 612 221 3 1 612 1 609 0.0 790
Msa0321650 MtrunA17_Chr4g0073741 63.328 619 220 6 1 617 1 614 0.0 788
Msa0321650 MtrunA17_Chr7g0223741 66.214 589 196 3 1 588 1 587 0.0 786
Msa0321650 MtrunA17_Chr7g0223621 63.072 612 223 3 1 612 1 609 0.0 784
Msa0321650 MtrunA17_Chr7g0248131 63.149 616 221 6 1 612 1 614 0.0 776
Msa0321650 MtrunA17_Chr7g0223631 64.542 612 200 5 1 612 1 595 0.0 774
Msa0321650 MtrunA17_Chr7g0248151 63.005 619 225 4 1 617 1 617 0.0 767
Msa0321650 MtrunA17_Chr7g0223701 61.452 620 229 5 1 612 1 618 0.0 764
Msa0321650 MtrunA17_Chr2g0293601 68.401 519 128 3 1 518 1 484 0.0 714
Msa0321650 MtrunA17_Chr1g0166551 61.463 615 123 5 6 619 2 503 0.0 712
Msa0321650 MtrunA17_Chr7g0223681 64.943 522 180 3 91 612 2 520 0.0 689
Msa0321650 MtrunA17_Chr7g0223541 62.752 545 199 4 74 617 31 572 0.0 686
Msa0321650 MtrunA17_Chr2g0331381 64.007 539 131 5 10 548 7 482 0.0 684
Msa0321650 MtrunA17_Chr6g0462601 57.290 583 243 5 1 581 1 579 0.0 660
Msa0321650 MtrunA17_Chr6g0462681 54.281 619 276 6 1 617 1 614 0.0 659
Msa0321650 MtrunA17_Chr2g0284681 54.045 618 258 8 5 614 4 603 0.0 635
Msa0321650 MtrunA17_Chr2g0293561 77.540 374 84 0 1 374 1 374 0.0 623
Msa0321650 MtrunA17_Chr6g0462711 53.247 616 277 7 9 617 10 621 0.0 620
Msa0321650 MtrunA17_Chr6g0462641 52.000 625 286 8 1 617 1 619 0.0 601
Msa0321650 MtrunA17_Chr8g0388761 51.946 591 271 9 7 592 36 618 0.0 599
Msa0321650 MtrunA17_Chr6g0462771 52.110 616 283 7 9 617 9 619 0.0 598
Msa0321650 MtrunA17_Chr8g0388671 51.864 590 273 8 7 592 36 618 0.0 597
Msa0321650 MtrunA17_Chr6g0462611 52.158 556 261 5 63 617 11 562 0.0 566
Msa0321650 MtrunA17_Chr2g0299821 64.796 392 134 3 1 390 1 390 1.24e-179 517
Msa0321650 MtrunA17_Chr2g0299821 48.077 104 45 3 444 538 379 482 6.02e-20 93.2
Msa0321650 MtrunA17_Chr2g0293271 60.641 437 101 4 21 457 43 408 3.55e-178 511
Msa0321650 MtrunA17_Chr2g0299921 68.020 394 122 2 3 396 1023 1412 3.87e-177 548
Msa0321650 MtrunA17_Chr2g0299921 67.095 389 123 3 3 390 1407 1791 4.80e-163 510
Msa0321650 MtrunA17_Chr7g0223761 70.185 379 69 4 241 618 3 338 5.63e-173 495
Msa0321650 MtrunA17_Chr2g0299791 66.324 389 127 2 1 389 1034 1418 2.83e-172 527
Msa0321650 MtrunA17_Chr2g0299851 65.385 390 131 2 1 390 1030 1415 4.24e-172 526
Msa0321650 MtrunA17_Chr1g0151991 76.632 291 68 0 1 291 1 291 7.12e-168 480
Msa0321650 MtrunA17_Chr2g0299881 64.194 391 132 3 3 389 1032 1418 1.72e-163 505
Msa0321650 MtrunA17_Chr2g0299881 58.904 73 28 1 180 250 1403 1475 4.90e-18 89.0
Msa0321650 MtrunA17_Chr6g0462861 44.623 623 311 11 6 617 6 605 3.67e-161 476
Msa0321650 MtrunA17_Chr2g0279901 47.576 557 268 12 10 560 57 595 5.91e-160 474
Msa0321650 MtrunA17_Chr7g0245521 77.656 273 61 0 1 273 1 273 5.14e-154 444
Msa0321650 MtrunA17_Chr4g0049501 43.922 617 309 17 10 614 54 645 5.39e-153 456
Msa0321650 MtrunA17_Chr2g0277021 44.643 560 288 11 8 561 70 613 7.66e-144 434
Msa0321650 MtrunA17_Chr7g0245531 66.454 313 103 2 305 617 2 312 2.11e-141 413
Msa0321650 MtrunA17_Chr1g0152001 67.213 305 97 3 315 619 1 302 1.82e-137 403
Msa0321650 MtrunA17_Chr1g0188611 60.606 297 109 2 322 618 1 289 2.91e-109 330
Msa0321650 MtrunA17_Chr1g0188601 78.534 191 41 0 92 282 1 191 1.77e-105 317
Msa0321650 MtrunA17_Chr7g0223671 75.510 196 44 1 74 265 8 203 8.51e-105 315
Msa0321650 MtrunA17_Chr6g0462631 51.603 312 108 3 100 410 22 291 4.18e-97 299
Msa0321650 MtrunA17_Chr6g0462751 47.262 347 123 4 67 410 12 301 3.63e-95 295
Msa0321650 MtrunA17_Chr7g0223771 86.928 153 20 0 93 245 1 153 1.24e-93 285
Msa0321650 MtrunA17_Chr5g0394391 32.681 511 322 12 9 505 29 531 1.42e-81 268
Msa0321650 MtrunA17_Chr7g0245541 61.386 202 77 1 418 619 1 201 1.47e-76 242
Msa0321650 MtrunA17_Chr4g0004331 32.965 452 283 7 10 445 4 451 1.80e-75 258
Msa0321650 MtrunA17_Chr4g0046881 79.433 141 26 1 135 275 14 151 2.13e-74 234
Msa0321650 MtrunA17_Chr7g0223861 83.969 131 21 0 78 208 4 134 4.20e-74 233
Msa0321650 MtrunA17_Chr2g0331901 32.045 440 283 6 10 437 4 439 1.83e-73 252
Msa0321650 MtrunA17_Chr6g0462791 61.714 175 66 1 109 283 2 175 4.30e-69 222
Msa0321650 MtrunA17_Chr3g0098761 31.591 421 281 6 10 429 4 418 2.19e-68 237
Msa0321650 MtrunA17_Chr5g0444001 31.935 429 283 6 10 433 4 428 1.10e-67 230
Msa0321650 MtrunA17_Chr4g0064621 32.115 520 320 15 9 504 140 650 7.18e-61 214
Msa0321650 MtrunA17_Chr4g0060241 30.859 512 304 18 10 497 21 506 2.85e-57 202
Msa0321650 MtrunA17_Chr3g0120101 26.886 517 340 11 12 495 29 540 9.96e-56 203
Msa0321650 MtrunA17_Chr5g0394221 37.658 316 176 8 280 581 40 348 5.31e-53 185
Msa0321650 MtrunA17_Chr6g0462701 53.801 171 55 1 100 270 22 168 2.58e-52 177
Msa0321650 MtrunA17_Chr6g0481391 68.033 122 36 1 38 159 13 131 1.70e-49 168
Msa0321650 MtrunA17_Chr5g0442171 31.307 329 206 8 170 488 1 319 1.30e-44 161
Msa0321650 MtrunA17_Chr4g0046871 73.786 103 26 1 336 438 6 107 2.37e-43 150
Msa0321650 MtrunA17_Chr6g0462691 52.448 143 66 2 322 463 1 142 2.77e-40 144
Msa0321650 MtrunA17_Chr7g0223871 57.031 128 55 0 492 619 7 134 1.48e-38 139
Msa0321650 MtrunA17_Chr5g0394231 37.004 227 92 4 74 299 5 181 7.17e-37 135
Msa0321650 MtrunA17_Chr7g0223791 50.000 132 57 1 442 573 58 180 9.46e-33 124
Msa0321650 MtrunA17_Chr7g0223791 62.195 82 23 3 56 137 18 91 8.63e-23 95.9
Msa0321650 MtrunA17_Chr6g0481381 74.324 74 19 0 176 249 1 74 2.65e-32 123
Msa0321650 MtrunA17_Chr6g0481381 58.333 96 40 0 516 611 75 170 1.63e-28 112
Msa0321650 MtrunA17_Chr1g0188591 75.342 73 18 0 1 73 1 73 2.86e-31 117
Msa0321650 MtrunA17_Chr7g0223691 69.863 73 22 0 1 73 1 73 1.20e-30 114
Msa0321650 MtrunA17_Chr7g0223881 57.317 82 34 1 281 362 2 82 6.30e-26 101
Msa0321650 MtrunA17_Chr6g0462621 63.014 73 25 1 1 73 1 71 6.96e-24 95.5
Msa0321650 MtrunA17_Chr7g0223661 48.333 120 18 2 315 434 1 76 5.23e-23 93.6
Msa0321650 MtrunA17_Chr7g0221651 48.193 83 43 0 215 297 1 83 6.28e-21 87.8
Msa0321650 MtrunA17_Chr6g0462801 62.121 66 20 1 322 382 1 66 5.72e-18 78.6
Msa0321650 MtrunA17_Chr5g0442161 31.373 153 98 4 9 157 28 177 1.43e-16 78.2
Msa0321650 MtrunA17_Chr2g0299891 71.111 45 13 0 315 359 1 45 5.20e-16 73.2
Msa0321650 MtrunA17_Chr4g0064651 41.935 93 52 2 397 488 29 120 7.41e-15 71.6
Msa0321650 MtrunA17_Chr2g0284661 51.190 84 39 2 368 450 61 143 1.07e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321650 AT3G12580.1 64.943 619 211 5 1 617 1 615 0.0 810
Msa0321650 AT5G02500.1 64.297 619 215 5 1 617 1 615 0.0 801
Msa0321650 AT5G02490.1 63.328 619 221 5 1 617 1 615 0.0 789
Msa0321650 AT3G09440.1 62.843 619 224 5 1 617 1 615 0.0 780
Msa0321650 AT3G09440.4 62.843 619 224 5 1 617 1 615 0.0 780
Msa0321650 AT3G09440.2 62.843 619 224 5 1 617 1 615 0.0 780
Msa0321650 AT3G09440.3 62.843 619 224 5 1 617 1 615 0.0 780
Msa0321650 AT1G16030.1 62.843 619 223 6 1 617 1 614 0.0 778
Msa0321650 AT1G56410.1 63.005 619 221 6 1 617 1 613 0.0 769
Msa0321650 AT5G28540.1 51.713 613 284 9 7 615 35 639 0.0 612
Msa0321650 AT5G42020.1 51.550 613 285 9 7 615 35 639 0.0 610
Msa0321650 AT5G42020.3 51.550 613 285 9 7 615 40 644 0.0 609
Msa0321650 AT5G02500.2 73.779 389 100 1 1 389 1 387 0.0 605
Msa0321650 AT1G09080.2 53.401 588 264 7 7 591 40 620 0.0 593
Msa0321650 AT1G09080.1 53.401 588 264 7 7 591 50 630 0.0 593
Msa0321650 AT5G42020.2 51.142 569 244 12 7 555 35 589 0.0 536
Msa0321650 AT5G09590.1 46.416 558 274 14 10 560 60 599 3.92e-149 447
Msa0321650 AT4G37910.1 45.601 557 280 11 10 560 55 594 8.23e-146 438
Msa0321650 AT4G37910.2 45.601 557 280 11 10 560 55 594 8.23e-146 438
Msa0321650 AT4G24280.1 44.286 560 290 11 8 561 79 622 2.57e-144 436
Msa0321650 AT5G49910.1 44.643 560 288 11 8 561 79 622 8.13e-144 434
Msa0321650 AT2G32120.1 33.463 514 309 12 9 508 30 524 7.89e-80 263
Msa0321650 AT2G32120.2 33.463 514 309 12 9 508 30 524 7.89e-80 263
Msa0321650 AT1G79930.2 32.522 452 285 6 10 445 4 451 3.29e-76 259
Msa0321650 AT1G79930.1 32.522 452 285 6 10 445 4 451 6.98e-76 259
Msa0321650 AT1G79920.3 32.522 452 285 6 10 445 4 451 4.17e-75 254
Msa0321650 AT1G79920.2 32.522 452 285 6 10 445 4 451 4.17e-75 254
Msa0321650 AT1G79920.4 32.522 452 285 6 10 445 4 451 4.17e-75 254
Msa0321650 AT1G79920.1 32.522 452 285 6 10 445 4 451 4.67e-75 254
Msa0321650 AT1G11660.4 34.321 405 258 6 10 411 4 403 5.45e-72 247
Msa0321650 AT1G11660.3 34.321 405 258 6 10 411 4 403 5.45e-72 247
Msa0321650 AT1G11660.1 34.321 405 258 6 10 411 4 403 5.45e-72 247
Msa0321650 AT1G11660.2 34.688 369 235 4 46 411 1 366 1.23e-65 229
Msa0321650 AT4G16660.1 27.290 524 340 13 6 495 23 539 4.49e-58 210
Msa0321650 AT4G16660.2 27.290 524 340 13 6 495 47 563 6.45e-58 209

Find 149 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAATAGTTCCTGCACTTT+TGG 0.163788 2_4:+23181991 None:intergenic
GGAGATTGCAGAGGCTTATT+TGG 0.170727 2_4:-23182643 Msa0321650:CDS
ATGGATGCTGGTGCCATTGC+TGG 0.234093 2_4:-23182552 Msa0321650:CDS
CCTGATGAGGCTGTTGCTTA+TGG 0.243454 2_4:-23181928 Msa0321650:CDS
GTGTCTCTCCTCACAATTAA+AGG 0.259274 2_4:-23182402 Msa0321650:CDS
TCAGATGCTAAGAGGTTAAT+TGG 0.263493 2_4:-23182828 Msa0321650:intron
TTTGAAGTTAAAGCCACTTC+TGG 0.288192 2_4:-23182372 Msa0321650:CDS
AAGGACACTCTCTTTCAAAT+TGG 0.288998 2_4:-23182208 Msa0321650:CDS
ACTTTGTGCAAGAATTTAAT+AGG 0.318140 2_4:-23182302 Msa0321650:CDS
ATGACTGCATTTACAAGTTA+AGG 0.328913 2_4:-23181396 Msa0321650:CDS
AAGGAATGCTCTAAAGAATA+AGG 0.330434 2_4:-23181377 Msa0321650:CDS
TATCTTTAGAAGTAAGATAT+TGG 0.330640 2_4:+23181743 None:intergenic
TCTTCATACACTCATTAAAA+AGG 0.345773 2_4:+23182097 None:intergenic
GGATAACCTTTCTTTGTCAT+TGG 0.352083 2_4:+23181513 None:intergenic
AACGCACAAAACACCGTCTT+TGG 0.355144 2_4:-23183688 Msa0321650:intron
TGGCCGTTCAAGGTTATTGC+TGG 0.368855 2_4:-23182762 Msa0321650:CDS
TACATGATATTGTTCTTGTC+GGG 0.378162 2_4:-23182029 Msa0321650:CDS
CTGATGAGGCTGTTGCTTAT+GGG 0.384178 2_4:-23181927 Msa0321650:CDS
CTACAATCAAGGCACTATAT+TGG 0.386508 2_4:+23181719 None:intergenic
CATATTCATGTGTTGGTGTT+TGG 0.387460 2_4:-23183837 Msa0321650:CDS
CTCAACCTCCTCAAGGCTCT+TGG 0.389032 2_4:+23182252 None:intergenic
ACGTCTCGCAGCACCAAGTT+TGG 0.390269 2_4:+23181859 None:intergenic
CCATAAGCAACAGCCTCATC+AGG 0.390857 2_4:+23181928 None:intergenic
CATTAAGAATGTTCCAAACT+TGG 0.396244 2_4:-23181872 Msa0321650:CDS
CCCATTGTCACTTGGTATCT+CGG 0.399514 2_4:-23181833 Msa0321650:CDS
TGAGATTGATTATTTATATG+AGG 0.407076 2_4:-23182175 Msa0321650:CDS
GTACATGATATTGTTCTTGT+CGG 0.419451 2_4:-23182030 Msa0321650:CDS
TTTAAATTTGGTCTATGCTA+TGG 0.420325 2_4:+23184125 None:intergenic
TCATTACCTTGGCCTTCCTT+AGG 0.431184 2_4:+23181428 None:intergenic
TATATTGTTGTGGCCGTTCA+AGG 0.432647 2_4:-23182772 Msa0321650:CDS
CCGAGATACCAAGTGACAAT+GGG 0.444390 2_4:+23181833 None:intergenic
CTATTGCGTAACTTCTTCAA+TGG 0.452833 2_4:-23181976 Msa0321650:CDS
TAAATAATCAATCTCAATAG+TGG 0.454560 2_4:+23182182 None:intergenic
TTGTCTTTGATCTTGGTGGT+GGG 0.456314 2_4:-23182434 Msa0321650:CDS
TTTAAATTTGGTCTATGCTA+CGG 0.456880 2_4:+23184185 None:intergenic
TGATTATCCTCACAACGTTA+AGG 0.457564 2_4:+23182529 None:intergenic
TTTGTCTTTGATCTTGGTGG+TGG 0.458128 2_4:-23182435 Msa0321650:CDS
GTTGAGAACTTCTTGTGAAA+AGG 0.462126 2_4:-23182235 Msa0321650:CDS
ACTGCTGCAGCTATTGCATA+TGG 0.476452 2_4:-23182498 Msa0321650:CDS
CTTCTGGAAACACTCACCTC+GGG 0.477285 2_4:-23182356 Msa0321650:CDS
TGTTCCTCGTGGCCATCCCT+TGG 0.477913 2_4:-23181623 Msa0321650:CDS
TTTAGAAGTAAGATATTGGC+TGG 0.480975 2_4:+23181747 None:intergenic
TATTGCGTAACTTCTTCAAT+GGG 0.481757 2_4:-23181975 Msa0321650:CDS
CAAGATTGGCATGGTAATGT+TGG 0.483132 2_4:+23181036 None:intergenic
TTCTTGAGGATCATCTGAAA+GGG 0.487611 2_4:-23181249 Msa0321650:CDS
ATCTAATGCATTCATTACCT+TGG 0.490089 2_4:+23181417 None:intergenic
TTATGAGTGTGGTGATTCCT+AGG 0.490594 2_4:-23181795 Msa0321650:CDS
CTTCTTGTGAAAAGGCGAAA+AGG 0.494676 2_4:-23182227 Msa0321650:CDS
TGAGAATCGTTAAAATAAGC+AGG 0.500441 2_4:+23182588 None:intergenic
GGATGGCCACGAGGAACACT+TGG 0.501644 2_4:+23181628 None:intergenic
TTCATGTGTTGGTGTTTGGT+TGG 0.501933 2_4:-23183833 Msa0321650:CDS
CGAAAAGCCACCATGGATGC+TGG 0.502548 2_4:-23182564 Msa0321650:CDS
TTTCACTGATGCTCAAAGGT+TGG 0.504490 2_4:-23183743 Msa0321650:CDS
TATTGGCTGGTCTTCTTGAA+AGG 0.508102 2_4:+23181760 None:intergenic
ACGAGGAACACTTGGAAGAC+CGG 0.508128 2_4:+23181636 None:intergenic
GTTGGTCGGTGGTGCTGCTA+AGG 0.508209 2_4:-23183725 Msa0321650:CDS
GAGATTATCCACAACGATGA+AGG 0.511349 2_4:-23183793 Msa0321650:CDS
GCCTTGATTGTAGTTTGTGA+GGG 0.515295 2_4:-23181709 Msa0321650:CDS
TGATGAGGCTGTTGCTTATG+GGG 0.522303 2_4:-23181926 Msa0321650:CDS
AAAATTGATTCAAGAAGCTA+AGG 0.522479 2_4:-23181476 Msa0321650:CDS
TGCTTTGCTATCGATGAAAA+CGG 0.522627 2_4:-23181595 Msa0321650:CDS
TTGCTTTCACTGATGCTCAA+AGG 0.524988 2_4:-23183747 Msa0321650:CDS
ATGCTAAGAGGTTAATTGGT+AGG 0.525498 2_4:-23182824 Msa0321650:CDS
CGATAACCAATGACAAAGAA+AGG 0.526916 2_4:-23181519 Msa0321650:CDS
GTCTAGGATTCCAAAAGTGC+AGG 0.534926 2_4:-23182001 Msa0321650:CDS
GAACATCTACTTCATTCCCC+TGG 0.535646 2_4:+23181269 None:intergenic
ACTAGCCAGTCTTGATTACA+AGG 0.537318 2_4:+23181206 None:intergenic
GAGGTGAGTGTTTCCAGAAG+TGG 0.537456 2_4:+23182359 None:intergenic
AAAGGTACAGAAGTGTTCCT+AGG 0.538214 2_4:+23181778 None:intergenic
ACCCTCACAAACTACAATCA+AGG 0.539796 2_4:+23181708 None:intergenic
AGCAATGGCACCAGCATCCA+TGG 0.539868 2_4:+23182554 None:intergenic
TGCCTTGATTGTAGTTTGTG+AGG 0.542190 2_4:-23181710 Msa0321650:CDS
GACGTTACCCCATTGTCACT+TGG 0.546373 2_4:-23181841 Msa0321650:CDS
TCAGTGAAAGCAACAAAAGA+AGG 0.546424 2_4:+23183757 None:intergenic
GTGAGTGACAACAACATGCT+TGG 0.547808 2_4:-23181673 Msa0321650:CDS
CGTGCAGTGGGAATCGACCT+TGG 0.550120 2_4:-23183865 Msa0321650:CDS
TTGTAGTTTGTGAGGGTGAG+AGG 0.551491 2_4:-23181702 Msa0321650:CDS
AGAGGCTTATTTGGAATCAC+CGG 0.553942 2_4:-23182634 Msa0321650:CDS
AAACAACCCCTCGAAAACTT+CGG 0.555908 2_4:-23184220 None:intergenic
GTTCTTGAGGATCATCTGAA+AGG 0.557323 2_4:-23181250 Msa0321650:CDS
CTTGAAAGACACACAAACTT+CGG 0.559044 2_4:-23184160 Msa0321650:five_prime_UTR
GATCTCATTCTTACTTCCAG+TGG 0.560329 2_4:+23181543 None:intergenic
TCTGCAGAGGAAAGATCCAC+TGG 0.563089 2_4:-23181559 Msa0321650:CDS
ACATGATATTGTTCTTGTCG+GGG 0.563522 2_4:-23182028 Msa0321650:CDS
GAAAGGGCTTGAGAGTATGT+TGG 0.565506 2_4:-23181233 Msa0321650:CDS
GAACACCTTGTAATCAAGAC+TGG 0.565696 2_4:-23181211 Msa0321650:CDS
AAACATGAAGGTCGTGCAGT+GGG 0.567739 2_4:-23183877 Msa0321650:CDS
GATTGTTGAAAGTTGTCTTG+TGG 0.568361 2_4:-23182076 Msa0321650:CDS
CAATCTCCTTCATGTTTGTG+AGG 0.570747 2_4:+23182658 None:intergenic
GCGTAACTTCTTCAATGGGA+AGG 0.571597 2_4:-23181971 Msa0321650:CDS
TACGATTAGTGTTAAGTACA+AGG 0.571844 2_4:-23182724 Msa0321650:CDS
GGAATTTCTCATCTTCAGCA+TGG 0.578307 2_4:+23181449 None:intergenic
TGTAGTTTGTGAGGGTGAGA+GGG 0.579578 2_4:-23181701 Msa0321650:CDS
CATGATATTGTTCTTGTCGG+GGG 0.579632 2_4:-23182027 Msa0321650:CDS
TAGTGTTAAGTACAAGGGTG+AGG 0.581461 2_4:-23182718 Msa0321650:CDS
ACTGATGCTCAAAGGTTGGT+CGG 0.583319 2_4:-23183739 Msa0321650:CDS
ACTTCTGGAAACACTCACCT+CGG 0.583908 2_4:-23182357 Msa0321650:CDS
TTGCTGGCCTTAACGTTGTG+AGG 0.589688 2_4:-23182536 Msa0321650:CDS
GAATAGAAATATACCAAAGA+CGG 0.590465 2_4:+23183675 None:intergenic
ACCGAGATACCAAGTGACAA+TGG 0.590955 2_4:+23181832 None:intergenic
TTGTACTTAACACTAATCGT+AGG 0.591476 2_4:+23182726 None:intergenic
GGCTCTTGTGATTGAACGAG+AGG 0.591760 2_4:+23184047 None:intergenic
CTGAAGATGAGAAATTCCTA+AGG 0.592200 2_4:-23181444 Msa0321650:CDS
ATATGCAATAGCTGCAGCAG+TGG 0.594040 2_4:+23182500 None:intergenic
CATCTTAACTGTTTCTGCAG+AGG 0.599860 2_4:-23181572 Msa0321650:CDS
CTTCATGTTTGTGAGGACAA+TGG 0.600128 2_4:+23182665 None:intergenic
AGAACAAAGTGGATATCACT+AGG 0.600332 2_4:-23182278 Msa0321650:CDS
TATGCAATAGCTGCAGCAGT+GGG 0.602359 2_4:+23182501 None:intergenic
GACAATGGATGAGATTTCCT+CGG 0.602552 2_4:+23182680 None:intergenic
AGATGAGAAATTCCTAAGGA+AGG 0.602756 2_4:-23181440 Msa0321650:CDS
CACATGAATATGTTGTTCCA+AGG 0.603031 2_4:+23183848 None:intergenic
CATTGTCCTCACAAACATGA+AGG 0.603324 2_4:-23182664 Msa0321650:CDS
TTGCTTCATAAGAATAACCA+GGG 0.605023 2_4:-23181286 Msa0321650:CDS
GATAGCAAAGCATACATCCA+AGG 0.607442 2_4:+23181606 None:intergenic
CACAACGTTAAGGCCAGCAA+TGG 0.608818 2_4:+23182539 None:intergenic
ATTTAATAGGAAGAACAAAG+TGG 0.609345 2_4:-23182289 Msa0321650:CDS
GGAACAACATATTCATGTGT+TGG 0.609685 2_4:-23183844 Msa0321650:CDS
TTTGCTTCATAAGAATAACC+AGG 0.610100 2_4:-23181287 Msa0321650:CDS
AGACATCACCTTTAATTGTG+AGG 0.611395 2_4:+23182394 None:intergenic
ACACCAGCAATAACCTTGAA+CGG 0.614534 2_4:+23182759 None:intergenic
GAATGAAGTAGATGTTCTTG+AGG 0.618161 2_4:-23181263 Msa0321650:CDS
ATAGCAAAGCATACATCCAA+GGG 0.619096 2_4:+23181607 None:intergenic
TTCTCAGCGAAAAGCCACCA+TGG 0.620029 2_4:-23182571 Msa0321650:CDS
AAAACATGAAGGTCGTGCAG+TGG 0.622170 2_4:-23183878 Msa0321650:CDS
TTCTGGAAACACTCACCTCG+GGG 0.622900 2_4:-23182355 Msa0321650:CDS
CAAAGCATACATCCAAGGGA+TGG 0.629656 2_4:+23181611 None:intergenic
CAAGTTTGAAGAAATCAACA+TGG 0.636916 2_4:-23182121 Msa0321650:CDS
CATAAGCAACAGCCTCATCA+GGG 0.640869 2_4:+23181929 None:intergenic
AGGTTGGACAAAATTTGCAG+TGG 0.644500 2_4:-23181098 Msa0321650:three_prime_UTR
AGAAGTTCTCAACCTCCTCA+AGG 0.646239 2_4:+23182245 None:intergenic
GAGCCAGCACAAGAAAACAA+AGG 0.647648 2_4:-23184029 Msa0321650:intron
GGAGAAGCTCCTTTGTGCCG+AGG 0.650523 2_4:-23182697 Msa0321650:CDS
GACAAAAGAAGTAATTGTGA+TGG 0.650690 2_4:-23182471 Msa0321650:CDS
ATGAGATTTCCTCGGCACAA+AGG 0.653122 2_4:+23182688 None:intergenic
CTAGGAATCCAAGAGCCTTG+AGG 0.655232 2_4:-23182260 Msa0321650:CDS
CAAGAGCATCAACCCTGATG+AGG 0.657948 2_4:-23181941 Msa0321650:CDS
GAAATTCCTAAGGAAGGCCA+AGG 0.662766 2_4:-23181434 Msa0321650:CDS
GGAATCCAAGAGCCTTGAGG+AGG 0.663076 2_4:-23182257 Msa0321650:CDS
GATCAATCGTGCAATTACTG+TGG 0.674376 2_4:-23181317 Msa0321650:CDS
ACGATTAGTGTTAAGTACAA+GGG 0.676520 2_4:-23182723 Msa0321650:CDS
TAATGATCGTATTATGAGTG+TGG 0.677159 2_4:-23181806 Msa0321650:CDS
AAACATGAAGGAGATTGCAG+AGG 0.695775 2_4:-23182652 Msa0321650:CDS
ACATCCAAGGGATGGCCACG+AGG 0.704531 2_4:+23181619 None:intergenic
GATGCTCAAAGGTTGGTCGG+TGG 0.706549 2_4:-23183736 Msa0321650:CDS
CGAGATACCAAGTGACAATG+GGG 0.706870 2_4:+23181834 None:intergenic
GGTCTTCCAAGTGTTCCTCG+TGG 0.707924 2_4:-23181634 Msa0321650:CDS
TGCTTCATAAGAATAACCAG+GGG 0.713870 2_4:-23181285 Msa0321650:CDS
TCTGGAAACACTCACCTCGG+GGG 0.720115 2_4:-23182354 Msa0321650:CDS
AATGGCACCAGCATCCATGG+TGG 0.736459 2_4:+23182557 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACATTTCTTAAAT+AGG + chr2_4:23181297-23181316 None:intergenic 10.0%
!!! ATTTAAAAATTATTTGAGTT+AGG - chr2_4:23181690-23181709 Msa0321650:CDS 10.0%
!!! TATATATATTAGTATTTTGA+AGG - chr2_4:23184105-23184124 Msa0321650:five_prime_UTR 10.0%
!! ATGAGTAAATATTAAAAGAT+TGG + chr2_4:23181575-23181594 None:intergenic 15.0%
!! TAAAGAAAAACAATTTCTTT+GGG + chr2_4:23181905-23181924 None:intergenic 15.0%
!! ATAGATGGATAAATAAATAA+AGG - chr2_4:23182100-23182119 Msa0321650:CDS 15.0%
!! ATGAATTAATATATATGTAG+CGG - chr2_4:23182226-23182245 Msa0321650:CDS 15.0%
!!! TGATTCTTTATTTTCAAATG+TGG - chr2_4:23181729-23181748 Msa0321650:CDS 20.0%
!! GTAAAGAAAAACAATTTCTT+TGG + chr2_4:23181906-23181925 None:intergenic 20.0%
!!! TTAAAGTTGATCATAATAGA+TGG - chr2_4:23182085-23182104 Msa0321650:CDS 20.0%
!!! AATATATCTTTTTGAACAAC+GGG + chr2_4:23182437-23182456 None:intergenic 20.0%
!!! CAATATATCTTTTTGAACAA+CGG + chr2_4:23182438-23182457 None:intergenic 20.0%
!! TTCAAAAAGATATATTGTTG+TGG - chr2_4:23182441-23182460 Msa0321650:CDS 20.0%
!!! AATATTTTTGTCTTTGATCT+TGG - chr2_4:23182782-23182801 Msa0321650:CDS 20.0%
!! TAAATAATCAATCTCAATAG+TGG + chr2_4:23183044-23183063 None:intergenic 20.0%
!! TGAGATTGATTATTTATATG+AGG - chr2_4:23183048-23183067 Msa0321650:intron 20.0%
!!! TATCTTTAGAAGTAAGATAT+TGG + chr2_4:23183483-23183502 None:intergenic 20.0%
!!! TTTTATTCTTTTGAGAAGAA+AGG + chr2_4:23183885-23183904 None:intergenic 20.0%
!!! TATATTAGTATTTTGAAGGT+TGG - chr2_4:23184109-23184128 Msa0321650:five_prime_UTR 20.0%
! TTTAAATTTGGTCTATGCTA+CGG + chr2_4:23181041-23181060 None:intergenic 25.0%
!! GTCTTTCAAGCTTTTAAATT+TGG + chr2_4:23181053-23181072 None:intergenic 25.0%
! TTTAAATTTGGTCTATGCTA+TGG + chr2_4:23181041-23181060 None:intergenic 25.0%
!! GTCTTTCAAGCTTTTAAATT+TGG + chr2_4:23181053-23181072 None:intergenic 25.0%
! TTTGTATACCTGAAAGATTA+TGG - chr2_4:23181318-23181337 Msa0321650:CDS 25.0%
!!! TTTTTTTGCCATAATCTTTC+AGG + chr2_4:23181329-23181348 None:intergenic 25.0%
! ATTATGGCAAAAAAACATGA+AGG - chr2_4:23181334-23181353 Msa0321650:CDS 25.0%
! GAATAGAAATATACCAAAGA+CGG + chr2_4:23181551-23181570 None:intergenic 25.0%
! ATTTACTCATATTGTAGCTT+AGG - chr2_4:23181585-23181604 Msa0321650:CDS 25.0%
!! ATATATCTTTTTGAACAACG+GGG + chr2_4:23182436-23182455 None:intergenic 25.0%
! ACTTTGTGCAAGAATTTAAT+AGG - chr2_4:23182921-23182940 Msa0321650:intron 25.0%
! ATTTAATAGGAAGAACAAAG+TGG - chr2_4:23182934-23182953 Msa0321650:intron 25.0%
! TCTTCATACACTCATTAAAA+AGG + chr2_4:23183129-23183148 None:intergenic 25.0%
!! AAAATTGATTCAAGAAGCTA+AGG - chr2_4:23183747-23183766 Msa0321650:CDS 25.0%
!!! ATAAAGTTTTGTTGTGACAG+TGG - chr2_4:23181758-23181777 Msa0321650:CDS 30.0%
CTGATTGTATAACACATAAG+AGG - chr2_4:23181949-23181968 Msa0321650:CDS 30.0%
!!! ACTTTTGAAACTACGATATG+TGG + chr2_4:23182022-23182041 None:intergenic 30.0%
ATAGGTCGTAAGTTAGATTA+TGG - chr2_4:23182286-23182305 Msa0321650:CDS 30.0%
TATGACCATGTTAAAGAAAC+AGG - chr2_4:23182336-23182355 Msa0321650:CDS 30.0%
! ATTCACCTGTTTCTTTAACA+TGG + chr2_4:23182344-23182363 None:intergenic 30.0%
!!! TTTTTTCTTCAGATGCTAAG+AGG - chr2_4:23182387-23182406 Msa0321650:CDS 30.0%
TTGTACTTAACACTAATCGT+AGG + chr2_4:23182500-23182519 None:intergenic 30.0%
TACGATTAGTGTTAAGTACA+AGG - chr2_4:23182499-23182518 Msa0321650:CDS 30.0%
ACGATTAGTGTTAAGTACAA+GGG - chr2_4:23182500-23182519 Msa0321650:CDS 30.0%
TGAGAATCGTTAAAATAAGC+AGG + chr2_4:23182638-23182657 None:intergenic 30.0%
! CACAATTACTTCTTTTGTCA+AGG + chr2_4:23182751-23182770 None:intergenic 30.0%
GACAAAAGAAGTAATTGTGA+TGG - chr2_4:23182752-23182771 Msa0321650:CDS 30.0%
! ATTTTTGTCTTTGATCTTGG+TGG - chr2_4:23182785-23182804 Msa0321650:CDS 30.0%
CAAGTTTGAAGAAATCAACA+TGG - chr2_4:23183102-23183121 Msa0321650:intron 30.0%
GTACATGATATTGTTCTTGT+CGG - chr2_4:23183193-23183212 Msa0321650:intron 30.0%
TACATGATATTGTTCTTGTC+GGG - chr2_4:23183194-23183213 Msa0321650:intron 30.0%
TATTGCGTAACTTCTTCAAT+GGG - chr2_4:23183248-23183267 Msa0321650:intron 30.0%
CATTAAGAATGTTCCAAACT+TGG - chr2_4:23183351-23183370 Msa0321650:intron 30.0%
TAATGATCGTATTATGAGTG+TGG - chr2_4:23183417-23183436 Msa0321650:intron 30.0%
TTTAGAAGTAAGATATTGGC+TGG + chr2_4:23183479-23183498 None:intergenic 30.0%
!! CAATTTTTCAATTTCGACTC+TGG + chr2_4:23183734-23183753 None:intergenic 30.0%
ATCTAATGCATTCATTACCT+TGG + chr2_4:23183809-23183828 None:intergenic 30.0%
ATGACTGCATTTACAAGTTA+AGG - chr2_4:23183827-23183846 Msa0321650:CDS 30.0%
!! AAGGAATGCTCTAAAGAATA+AGG - chr2_4:23183846-23183865 Msa0321650:CDS 30.0%
TTTGCTTCATAAGAATAACC+AGG - chr2_4:23183936-23183955 Msa0321650:intron 30.0%
TTGCTTCATAAGAATAACCA+GGG - chr2_4:23183937-23183956 Msa0321650:intron 30.0%
CTTGAAAGACACACAAACTT+CGG - chr2_4:23181063-23181082 Msa0321650:three_prime_UTR 35.0%
CACATGAATATGTTGTTCCA+AGG + chr2_4:23181378-23181397 None:intergenic 35.0%
GGAACAACATATTCATGTGT+TGG - chr2_4:23181379-23181398 Msa0321650:CDS 35.0%
! CATATTCATGTGTTGGTGTT+TGG - chr2_4:23181386-23181405 Msa0321650:CDS 35.0%
TCAGTGAAAGCAACAAAAGA+AGG + chr2_4:23181469-23181488 None:intergenic 35.0%
AAGGTCCTAGATTCAAACAT+GGG - chr2_4:23181648-23181667 Msa0321650:CDS 35.0%
TTCTTTACTTCGACACTTCT+TGG - chr2_4:23181918-23181937 Msa0321650:CDS 35.0%
TGTGTTATACAATCAGACAC+CGG + chr2_4:23181945-23181964 None:intergenic 35.0%
GGACAATTAAGACAAATGTC+TGG - chr2_4:23181978-23181997 Msa0321650:CDS 35.0%
TCAGATGCTAAGAGGTTAAT+TGG - chr2_4:23182395-23182414 Msa0321650:CDS 35.0%
ATGCTAAGAGGTTAATTGGT+AGG - chr2_4:23182399-23182418 Msa0321650:CDS 35.0%
! GTGACAACTGCATTTTTAAC+CGG + chr2_4:23182611-23182630 None:intergenic 35.0%
TGATTATCCTCACAACGTTA+AGG + chr2_4:23182697-23182716 None:intergenic 35.0%
AGACATCACCTTTAATTGTG+AGG + chr2_4:23182832-23182851 None:intergenic 35.0%
! TTTGAAGTTAAAGCCACTTC+TGG - chr2_4:23182851-23182870 Msa0321650:intron 35.0%
AGAACAAAGTGGATATCACT+AGG - chr2_4:23182945-23182964 Msa0321650:intron 35.0%
GTTGAGAACTTCTTGTGAAA+AGG - chr2_4:23182988-23183007 Msa0321650:intron 35.0%
AAGGACACTCTCTTTCAAAT+TGG - chr2_4:23183015-23183034 Msa0321650:intron 35.0%
GATTGTTGAAAGTTGTCTTG+TGG - chr2_4:23183147-23183166 Msa0321650:intron 35.0%
ACATGATATTGTTCTTGTCG+GGG - chr2_4:23183195-23183214 Msa0321650:intron 35.0%
CTATTGCGTAACTTCTTCAA+TGG - chr2_4:23183247-23183266 Msa0321650:intron 35.0%
! CTACAATCAAGGCACTATAT+TGG + chr2_4:23183507-23183526 None:intergenic 35.0%
!!! CTTGGTTTTTTCAGTCTTTC+CGG - chr2_4:23183568-23183587 Msa0321650:intron 35.0%
ATAGCAAAGCATACATCCAA+GGG + chr2_4:23183619-23183638 None:intergenic 35.0%
! TGCTTTGCTATCGATGAAAA+CGG - chr2_4:23183628-23183647 Msa0321650:intron 35.0%
CGATAACCAATGACAAAGAA+AGG - chr2_4:23183704-23183723 Msa0321650:CDS 35.0%
GGATAACCTTTCTTTGTCAT+TGG + chr2_4:23183713-23183732 None:intergenic 35.0%
CTGAAGATGAGAAATTCCTA+AGG - chr2_4:23183779-23183798 Msa0321650:CDS 35.0%
AGATGAGAAATTCCTAAGGA+AGG - chr2_4:23183783-23183802 Msa0321650:CDS 35.0%
TGCTTCATAAGAATAACCAG+GGG - chr2_4:23183938-23183957 Msa0321650:intron 35.0%
GAATGAAGTAGATGTTCTTG+AGG - chr2_4:23183960-23183979 Msa0321650:intron 35.0%
TTCTTGAGGATCATCTGAAA+GGG - chr2_4:23183974-23183993 Msa0321650:intron 35.0%
! ACAGTCTTTTAGCACAAGAT+TGG + chr2_4:23184204-23184223 None:intergenic 35.0%
!!! CTACCTTTGTTTTCTTGTGC+TGG + chr2_4:23181200-23181219 None:intergenic 40.0%
! TTCATGTGTTGGTGTTTGGT+TGG - chr2_4:23181390-23181409 Msa0321650:CDS 40.0%
GAGATTATCCACAACGATGA+AGG - chr2_4:23181430-23181449 Msa0321650:CDS 40.0%
! TTTTGTTGCCTTCATCGTTG+TGG + chr2_4:23181441-23181460 None:intergenic 40.0%
TTGCTTTCACTGATGCTCAA+AGG - chr2_4:23181476-23181495 Msa0321650:CDS 40.0%
TTTCACTGATGCTCAAAGGT+TGG - chr2_4:23181480-23181499 Msa0321650:CDS 40.0%
CAAGGTCCTAGATTCAAACA+TGG - chr2_4:23181647-23181666 Msa0321650:CDS 40.0%
CTTCACCCATGTTTGAATCT+AGG + chr2_4:23181656-23181675 None:intergenic 40.0%
GCTTCATAGGTTACGACATT+GGG + chr2_4:23181796-23181815 None:intergenic 40.0%
TGCTTCATAGGTTACGACAT+TGG + chr2_4:23181797-23181816 None:intergenic 40.0%
!! TAGAGGTGTCTATGCTTCAT+AGG + chr2_4:23181809-23181828 None:intergenic 40.0%
TATGAAGCATAGACACCTCT+AGG - chr2_4:23181808-23181827 Msa0321650:CDS 40.0%
ATAACACATAAGAGGCGAGT+TGG - chr2_4:23181957-23181976 Msa0321650:CDS 40.0%
TCAAGAAACGTTGATCAACG+AGG - chr2_4:23182052-23182071 Msa0321650:CDS 40.0%
CAAGAAACGTTGATCAACGA+GGG - chr2_4:23182053-23182072 Msa0321650:CDS 40.0%
AACTTACGACCTATGAAGCA+TGG + chr2_4:23182280-23182299 None:intergenic 40.0%
TATATTGTTGTGGCCGTTCA+AGG - chr2_4:23182451-23182470 Msa0321650:CDS 40.0%
ACACCAGCAATAACCTTGAA+CGG + chr2_4:23182467-23182486 None:intergenic 40.0%
TAGTGTTAAGTACAAGGGTG+AGG - chr2_4:23182505-23182524 Msa0321650:CDS 40.0%
GACAATGGATGAGATTTCCT+CGG + chr2_4:23182546-23182565 None:intergenic 40.0%
CTTCATGTTTGTGAGGACAA+TGG + chr2_4:23182561-23182580 None:intergenic 40.0%
CATTGTCCTCACAAACATGA+AGG - chr2_4:23182559-23182578 Msa0321650:CDS 40.0%
CAATCTCCTTCATGTTTGTG+AGG + chr2_4:23182568-23182587 None:intergenic 40.0%
AAACATGAAGGAGATTGCAG+AGG - chr2_4:23182571-23182590 Msa0321650:CDS 40.0%
! AGAGGCTTATTTGGAATCAC+CGG - chr2_4:23182589-23182608 Msa0321650:CDS 40.0%
TTTGTCTTTGATCTTGGTGG+TGG - chr2_4:23182788-23182807 Msa0321650:CDS 40.0%
! TTGTCTTTGATCTTGGTGGT+GGG - chr2_4:23182789-23182808 Msa0321650:CDS 40.0%
GTGTCTCTCCTCACAATTAA+AGG - chr2_4:23182821-23182840 Msa0321650:CDS 40.0%
CTTCTTGTGAAAAGGCGAAA+AGG - chr2_4:23182996-23183015 Msa0321650:intron 40.0%
CATGATATTGTTCTTGTCGG+GGG - chr2_4:23183196-23183215 Msa0321650:intron 40.0%
TTATGAGTGTGGTGATTCCT+AGG - chr2_4:23183428-23183447 Msa0321650:intron 40.0%
!! AAAGGTACAGAAGTGTTCCT+AGG + chr2_4:23183448-23183467 None:intergenic 40.0%
!! TATTGGCTGGTCTTCTTGAA+AGG + chr2_4:23183466-23183485 None:intergenic 40.0%
! TGCCTTGATTGTAGTTTGTG+AGG - chr2_4:23183513-23183532 Msa0321650:intron 40.0%
! GCCTTGATTGTAGTTTGTGA+GGG - chr2_4:23183514-23183533 Msa0321650:intron 40.0%
ACCCTCACAAACTACAATCA+AGG + chr2_4:23183518-23183537 None:intergenic 40.0%
GATAGCAAAGCATACATCCA+AGG + chr2_4:23183620-23183639 None:intergenic 40.0%
!! CATCTTAACTGTTTCTGCAG+AGG - chr2_4:23183651-23183670 Msa0321650:intron 40.0%
GATCTCATTCTTACTTCCAG+TGG + chr2_4:23183683-23183702 None:intergenic 40.0%
GGAATTTCTCATCTTCAGCA+TGG + chr2_4:23183777-23183796 None:intergenic 40.0%
GATCAATCGTGCAATTACTG+TGG - chr2_4:23183906-23183925 Msa0321650:intron 40.0%
GTTCTTGAGGATCATCTGAA+AGG - chr2_4:23183973-23183992 Msa0321650:intron 40.0%
GAACACCTTGTAATCAAGAC+TGG - chr2_4:23184012-23184031 Msa0321650:intron 40.0%
ACTAGCCAGTCTTGATTACA+AGG + chr2_4:23184020-23184039 None:intergenic 40.0%
!!! GACTGGCTAGTTTTCTTCAT+CGG - chr2_4:23184029-23184048 Msa0321650:intron 40.0%
AGGTTGGACAAAATTTGCAG+TGG - chr2_4:23184125-23184144 Msa0321650:five_prime_UTR 40.0%
! CAAGATTGGCATGGTAATGT+TGG + chr2_4:23184190-23184209 None:intergenic 40.0%
! CTTTTAGCACAAGATTGGCA+TGG + chr2_4:23184199-23184218 None:intergenic 40.0%
GAGCCAGCACAAGAAAACAA+AGG - chr2_4:23181194-23181213 Msa0321650:exon 45.0%
AAAACATGAAGGTCGTGCAG+TGG - chr2_4:23181345-23181364 Msa0321650:CDS 45.0%
AAACATGAAGGTCGTGCAGT+GGG - chr2_4:23181346-23181365 Msa0321650:CDS 45.0%
! ACTGATGCTCAAAGGTTGGT+CGG - chr2_4:23181484-23181503 Msa0321650:CDS 45.0%
AACGCACAAAACACCGTCTT+TGG - chr2_4:23181535-23181554 Msa0321650:CDS 45.0%
! GTTTGAATCTAGGACCTTGC+AGG + chr2_4:23181646-23181665 None:intergenic 45.0%
ACGTGTCATGCGAATGTCAT+AGG + chr2_4:23181860-23181879 None:intergenic 45.0%
TACTTCGACACTTCTTGGAC+CGG - chr2_4:23181923-23181942 Msa0321650:CDS 45.0%
TTCGTGTCAAGTGTTTGCGT+TGG - chr2_4:23182251-23182270 Msa0321650:CDS 45.0%
ATGAGATTTCCTCGGCACAA+AGG + chr2_4:23182538-23182557 None:intergenic 45.0%
GGAGATTGCAGAGGCTTATT+TGG - chr2_4:23182580-23182599 Msa0321650:CDS 45.0%
TATGCAATAGCTGCAGCAGT+GGG + chr2_4:23182725-23182744 None:intergenic 45.0%
ATATGCAATAGCTGCAGCAG+TGG + chr2_4:23182726-23182745 None:intergenic 45.0%
ACTGCTGCAGCTATTGCATA+TGG - chr2_4:23182725-23182744 Msa0321650:CDS 45.0%
! ACTTCTGGAAACACTCACCT+CGG - chr2_4:23182866-23182885 Msa0321650:intron 45.0%
AGAAGTTCTCAACCTCCTCA+AGG + chr2_4:23182981-23183000 None:intergenic 45.0%
! GTCTAGGATTCCAAAAGTGC+AGG - chr2_4:23183222-23183241 Msa0321650:intron 45.0%
! CGCAATAGTTCCTGCACTTT+TGG + chr2_4:23183235-23183254 None:intergenic 45.0%
GCGTAACTTCTTCAATGGGA+AGG - chr2_4:23183252-23183271 Msa0321650:intron 45.0%
CATAAGCAACAGCCTCATCA+GGG + chr2_4:23183297-23183316 None:intergenic 45.0%
! CTGATGAGGCTGTTGCTTAT+GGG - chr2_4:23183296-23183315 Msa0321650:intron 45.0%
! TGATGAGGCTGTTGCTTATG+GGG - chr2_4:23183297-23183316 Msa0321650:intron 45.0%
CGAGATACCAAGTGACAATG+GGG + chr2_4:23183392-23183411 None:intergenic 45.0%
CCGAGATACCAAGTGACAAT+GGG + chr2_4:23183393-23183412 None:intergenic 45.0%
! CCCATTGTCACTTGGTATCT+CGG - chr2_4:23183390-23183409 Msa0321650:intron 45.0%
ACCGAGATACCAAGTGACAA+TGG + chr2_4:23183394-23183413 None:intergenic 45.0%
! TTGTAGTTTGTGAGGGTGAG+AGG - chr2_4:23183521-23183540 Msa0321650:intron 45.0%
! TGTAGTTTGTGAGGGTGAGA+GGG - chr2_4:23183522-23183541 Msa0321650:intron 45.0%
GTGAGTGACAACAACATGCT+TGG - chr2_4:23183550-23183569 Msa0321650:intron 45.0%
CAAAGCATACATCCAAGGGA+TGG + chr2_4:23183615-23183634 None:intergenic 45.0%
GAAATTCCTAAGGAAGGCCA+AGG - chr2_4:23183789-23183808 Msa0321650:CDS 45.0%
! TCATTACCTTGGCCTTCCTT+AGG + chr2_4:23183798-23183817 None:intergenic 45.0%
GAACATCTACTTCATTCCCC+TGG + chr2_4:23183957-23183976 None:intergenic 45.0%
! GAAAGGGCTTGAGAGTATGT+TGG - chr2_4:23183990-23184009 Msa0321650:intron 45.0%
!!! TAATTTTTAAATTGTTAAAT+TGG + chr2_4:23181682-23181701 None:intergenic 5.0%
GGCTCTTGTGATTGAACGAG+AGG + chr2_4:23181179-23181198 None:intergenic 50.0%
!! ACCTCTAGGACTACGTGTGT+CGG - chr2_4:23181822-23181841 Msa0321650:CDS 50.0%
CGTTGGAGTCCATGCTTCAT+AGG - chr2_4:23182268-23182287 Msa0321650:CDS 50.0%
TGGCCGTTCAAGGTTATTGC+TGG - chr2_4:23182461-23182480 Msa0321650:CDS 50.0%
TTCTCAGCGAAAAGCCACCA+TGG - chr2_4:23182652-23182671 Msa0321650:CDS 50.0%
CACAACGTTAAGGCCAGCAA+TGG + chr2_4:23182687-23182706 None:intergenic 50.0%
!! TTGCTGGCCTTAACGTTGTG+AGG - chr2_4:23182687-23182706 Msa0321650:CDS 50.0%
GAGGTGAGTGTTTCCAGAAG+TGG + chr2_4:23182867-23182886 None:intergenic 50.0%
! CTTCTGGAAACACTCACCTC+GGG - chr2_4:23182867-23182886 Msa0321650:intron 50.0%
! TTCTGGAAACACTCACCTCG+GGG - chr2_4:23182868-23182887 Msa0321650:intron 50.0%
CTAGGAATCCAAGAGCCTTG+AGG - chr2_4:23182963-23182982 Msa0321650:intron 50.0%
!! CAAGAGCATCAACCCTGATG+AGG - chr2_4:23183282-23183301 Msa0321650:intron 50.0%
CCATAAGCAACAGCCTCATC+AGG + chr2_4:23183298-23183317 None:intergenic 50.0%
! CCTGATGAGGCTGTTGCTTA+TGG - chr2_4:23183295-23183314 Msa0321650:intron 50.0%
GACGTTACCCCATTGTCACT+TGG - chr2_4:23183382-23183401 Msa0321650:intron 50.0%
ACGAGGAACACTTGGAAGAC+CGG + chr2_4:23183590-23183609 None:intergenic 50.0%
TCTGCAGAGGAAAGATCCAC+TGG - chr2_4:23183664-23183683 Msa0321650:intron 50.0%
! GATGCTCAAAGGTTGGTCGG+TGG - chr2_4:23181487-23181506 Msa0321650:CDS 55.0%
!! CCTCTAGGACTACGTGTGTC+GGG - chr2_4:23181823-23181842 Msa0321650:CDS 55.0%
CGAAAAGCCACCATGGATGC+TGG - chr2_4:23182659-23182678 Msa0321650:CDS 55.0%
AATGGCACCAGCATCCATGG+TGG + chr2_4:23182669-23182688 None:intergenic 55.0%
AGCAATGGCACCAGCATCCA+TGG + chr2_4:23182672-23182691 None:intergenic 55.0%
!! ATGGATGCTGGTGCCATTGC+TGG - chr2_4:23182671-23182690 Msa0321650:CDS 55.0%
TCTGGAAACACTCACCTCGG+GGG - chr2_4:23182869-23182888 Msa0321650:intron 55.0%
TATCAAAGTCCTCTCCCCCG+AGG + chr2_4:23182886-23182905 None:intergenic 55.0%
GGAATCCAAGAGCCTTGAGG+AGG - chr2_4:23182966-23182985 Msa0321650:intron 55.0%
CTCAACCTCCTCAAGGCTCT+TGG + chr2_4:23182974-23182993 None:intergenic 55.0%
TTCTTGTCGGGGGATCGTCT+AGG - chr2_4:23183206-23183225 Msa0321650:intron 55.0%
ACGTCTCGCAGCACCAAGTT+TGG + chr2_4:23183367-23183386 None:intergenic 55.0%
GGTCTTCCAAGTGTTCCTCG+TGG - chr2_4:23183589-23183608 Msa0321650:intron 55.0%
CGTGCAGTGGGAATCGACCT+TGG - chr2_4:23181358-23181377 Msa0321650:CDS 60.0%
!! GTTGGTCGGTGGTGCTGCTA+AGG - chr2_4:23181498-23181517 Msa0321650:CDS 60.0%
GTGCGCGTGTGAGTGTGAGA+TGG + chr2_4:23181624-23181643 None:intergenic 60.0%
CCCGACACACGTAGTCCTAG+AGG + chr2_4:23181826-23181845 None:intergenic 60.0%
GGAGAAGCTCCTTTGTGCCG+AGG - chr2_4:23182526-23182545 Msa0321650:CDS 60.0%
AAACACTCACCTCGGGGGAG+AGG - chr2_4:23182874-23182893 Msa0321650:intron 60.0%
GGATGGCCACGAGGAACACT+TGG + chr2_4:23183598-23183617 None:intergenic 60.0%
TGTTCCTCGTGGCCATCCCT+TGG - chr2_4:23183600-23183619 Msa0321650:intron 60.0%
ACATCCAAGGGATGGCCACG+AGG + chr2_4:23183607-23183626 None:intergenic 60.0%
CACTCACACGCGCACCTGCA+AGG - chr2_4:23181629-23181648 Msa0321650:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 23181017 23184228 23181017 ID=Msa0321650;Name=Msa0321650
chr2_4 mRNA 23181017 23184228 23181017 ID=Msa0321650-mRNA-1;Parent=Msa0321650;Name=Msa0321650-mRNA-1;_AED=0.04;_eAED=0.04;_QI=202|1|1|1|0.5|0.66|3|190|619
chr2_4 exon 23181017 23182846 23181017 ID=Msa0321650-mRNA-1:exon:8459;Parent=Msa0321650-mRNA-1
chr2_4 exon 23183689 23183911 23183689 ID=Msa0321650-mRNA-1:exon:8458;Parent=Msa0321650-mRNA-1
chr2_4 exon 23184030 23184228 23184030 ID=Msa0321650-mRNA-1:exon:8457;Parent=Msa0321650-mRNA-1
chr2_4 five_prime_UTR 23184030 23184228 23184030 ID=Msa0321650-mRNA-1:five_prime_utr;Parent=Msa0321650-mRNA-1
chr2_4 five_prime_UTR 23183909 23183911 23183909 ID=Msa0321650-mRNA-1:five_prime_utr;Parent=Msa0321650-mRNA-1
chr2_4 CDS 23183689 23183908 23183689 ID=Msa0321650-mRNA-1:cds;Parent=Msa0321650-mRNA-1
chr2_4 CDS 23181207 23182846 23181207 ID=Msa0321650-mRNA-1:cds;Parent=Msa0321650-mRNA-1
chr2_4 three_prime_UTR 23181017 23181206 23181017 ID=Msa0321650-mRNA-1:three_prime_utr;Parent=Msa0321650-mRNA-1
Gene Sequence

>Msa0321650

ATGGCAAAAAAACATGAAGGTCGTGCAGTGGGAATCGACCTTGGAACAACATATTCATGTGTTGGTGTTTGGTTGGAAGAAAAAAACAGAGTTGAGATTATCCACAACGATGAAGGCAACAAAATTACACCTTCTTTTGTTGCTTTCACTGATGCTCAAAGGTTGGTCGGTGGTGCTGCTAAGGATCAAGCTGCTATCAACGCACAAAACACCGTCTTTGATGCTAAGAGGTTAATTGGTAGGAAATTTAGTGACCCCGTTGTTCAAAAAGATATATTGTTGTGGCCGTTCAAGGTTATTGCTGGTGTTAATGACAAACCTACGATTAGTGTTAAGTACAAGGGTGAGGAGAAGCTCCTTTGTGCCGAGGAAATCTCATCCATTGTCCTCACAAACATGAAGGAGATTGCAGAGGCTTATTTGGAATCACCGGTTAAAAATGCAGTTGTCACTGTTCCTGCTTATTTTAACGATTCTCAGCGAAAAGCCACCATGGATGCTGGTGCCATTGCTGGCCTTAACGTTGTGAGGATAATCAATGAACCCACTGCTGCAGCTATTGCATATGGCCTTGACAAAAGAAGTAATTGTGATGGTGAAAGAAATATTTTTGTCTTTGATCTTGGTGGTGGGACTTTTGATGTGTCTCTCCTCACAATTAAAGGTGATGTCTTTGAAGTTAAAGCCACTTCTGGAAACACTCACCTCGGGGGAGAGGACTTTGATAACAGAATGCTGAACTACTTTGTGCAAGAATTTAATAGGAAGAACAAAGTGGATATCACTAGGAATCCAAGAGCCTTGAGGAGGTTGAGAACTTCTTGTGAAAAGGCGAAAAGGACACTCTCTTTCAAATTGGTCACCACTATTGAGATTGATTATTTATATGAGGACATTGACTTCTCTACATCAATCACTCGTGTCAAGTTTGAAGAAATCAACATGGACCTTTTTAATGAGTGTATGAAGATTGTTGAAAGTTGTCTTGTGGATTCAAAGATCGACAAGAGCAGTGTACATGATATTGTTCTTGTCGGGGGATCGTCTAGGATTCCAAAAGTGCAGGAACTATTGCGTAACTTCTTCAATGGGAAGGAGTTGTGCAAGAGCATCAACCCTGATGAGGCTGTTGCTTATGGGGCAGCTGTGCAAGCTGCTGTGTTGAGTGAAGACATTAAGAATGTTCCAAACTTGGTGCTGCGAGACGTTACCCCATTGTCACTTGGTATCTCGGTACATAATGATCGTATTATGAGTGTGGTGATTCCTAGGAACACTTCTGTACCTTTCAAGAAGACCAGCCAATATCTTACTTCTAAAGATAACCAATATAGTGCCTTGATTGTAGTTTGTGAGGGTGAGAGGGCAAGAGTGAGTGACAACAACATGCTTGGTTTTTTCAGTCTTTCCGGTCTTCCAAGTGTTCCTCGTGGCCATCCCTTGGATGTATGCTTTGCTATCGATGAAAACGGCATCTTAACTGTTTCTGCAGAGGAAAGATCCACTGGAAGTAAGAATGAGATCACGATAACCAATGACAAAGAAAGGTTATCCAGAGTCGAAATTGAAAAATTGATTCAAGAAGCTAAGGAGTACCATGCTGAAGATGAGAAATTCCTAAGGAAGGCCAAGGTAATGAATGCATTAGATGACTGCATTTACAAGTTAAGGAATGCTCTAAAGAATAAGGATGTCAAATTAAACCTTTCTTCTCAAAAGAATAAAAAGATCAATCGTGCAATTACTGTGGCTAAAGATTTGCTTCATAAGAATAACCAGGGGAATGAAGTAGATGTTCTTGAGGATCATCTGAAAGGGCTTGAGAGTATGTTGGAACACCTTGTAATCAAGACTGGCTAG
Protein sequence

>Msa0321650

MAKKHEGRAVGIDLGTTYSCVGVWLEEKNRVEIIHNDEGNKITPSFVAFTDAQRLVGGAAKDQAAINAQNTVFDAKRLIGRKFSDPVVQKDILLWPFKVIAGVNDKPTISVKYKGEEKLLCAEEISSIVLTNMKEIAEAYLESPVKNAVVTVPAYFNDSQRKATMDAGAIAGLNVVRIINEPTAAAIAYGLDKRSNCDGERNIFVFDLGGGTFDVSLLTIKGDVFEVKATSGNTHLGGEDFDNRMLNYFVQEFNRKNKVDITRNPRALRRLRTSCEKAKRTLSFKLVTTIEIDYLYEDIDFSTSITRVKFEEINMDLFNECMKIVESCLVDSKIDKSSVHDIVLVGGSSRIPKVQELLRNFFNGKELCKSINPDEAVAYGAAVQAAVLSEDIKNVPNLVLRDVTPLSLGISVHNDRIMSVVIPRNTSVPFKKTSQYLTSKDNQYSALIVVCEGERARVSDNNMLGFFSLSGLPSVPRGHPLDVCFAIDENGILTVSAEERSTGSKNEITITNDKERLSRVEIEKLIQEAKEYHAEDEKFLRKAKVMNALDDCIYKLRNALKNKDVKLNLSSQKNKKINRAITVAKDLLHKNNQGNEVDVLEDHLKGLESMLEHLVIKTG*