AlfalfaGEDB | Msa0328550

M. sativa cultivar zhongmu-4 / Msa0328550

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328550	XP_003595952.1	88.278	273	12	1	1	253	1	273	3.07e-176	498

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328550	sp\|A4VCM0\|NAC45_ARATH	67.296	159	32	1	1	139	1	159	9.90e-76	240

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0328550	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0169860	Msa0328550	0.806202	9.224833e-50	-8.615850e-47
Msa0188830	Msa0328550	0.803957	2.720580e-49	-8.615850e-47
Msa0191250	Msa0328550	0.812950	3.275160e-51	-8.615850e-47
Msa0201070	Msa0328550	0.802015	6.856434e-49	-8.615850e-47
Msa0212990	Msa0328550	0.811396	7.148093e-51	-8.615850e-47
Msa0243710	Msa0328550	0.804222	2.395415e-49	-8.615850e-47
Msa0311860	Msa0328550	0.805621	1.221881e-49	-8.615850e-47
Msa0328550	Msa0480980	0.800909	1.155027e-48	-8.615850e-47
Msa0328550	Msa0484780	0.803707	3.065587e-49	-8.615850e-47
Msa0328550	Msa0648880	0.800847	1.189289e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328550	MtrunA17_Chr2g0309891	88.278	273	12	1	1	253	1	273	2.82e-180	498
Msa0328550	MtrunA17_Chr3g0109821	67.857	168	34	1	1	148	1	168	4.12e-78	248
Msa0328550	MtrunA17_Chr5g0433781	61.905	189	52	2	1	169	1	189	4.48e-78	248
Msa0328550	MtrunA17_Chr6g0481091	67.308	156	32	1	1	137	1	156	2.01e-75	229
Msa0328550	MtrunA17_Chr5g0400461	45.089	224	92	3	1	201	1	216	3.24e-61	196
Msa0328550	MtrunA17_Chr4g0054791	55.346	159	49	2	1	137	1	159	1.01e-56	184
Msa0328550	MtrunA17_Chr4g0056541	50.307	163	63	2	6	150	7	169	9.54e-55	180
Msa0328550	MtrunA17_Chr8g0372121	48.485	165	67	2	1	147	1	165	2.54e-54	177
Msa0328550	MtrunA17_Chr4g0018391	51.316	152	56	2	6	139	7	158	1.60e-53	176
Msa0328550	MtrunA17_Chr1g0200561	54.000	150	51	2	6	137	19	168	2.24e-53	176

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328550	AT1G54330.1	72.500	160	28	1	1	144	1	160	1.14e-80	244
Msa0328550	AT3G17730.1	71.154	156	26	1	1	137	1	156	1.19e-78	238
Msa0328550	AT1G65910.1	65.143	175	39	2	1	156	1	172	3.13e-77	245
Msa0328550	AT3G03200.1	67.296	159	32	1	1	139	1	159	1.01e-76	240
Msa0328550	AT5G17260.1	66.038	159	34	1	1	139	1	159	1.54e-75	237
Msa0328550	AT4G17980.1	62.319	138	48	2	1	134	1	138	6.54e-60	190
Msa0328550	AT4G17980.2	62.319	138	48	2	1	134	1	138	1.18e-59	191
Msa0328550	AT5G46590.2	61.481	135	49	2	1	132	1	135	1.23e-58	187
Msa0328550	AT5G46590.1	61.481	135	49	2	1	132	1	135	1.58e-58	188
Msa0328550	AT5G66300.1	53.704	162	56	3	5	147	11	172	1.16e-55	180

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Find 81 sgRNAs with CRISPR-Local

Find 348 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGAAAGCTTCTTCTT+TGG	0.243238	2_4:+41149090	None:intergenic
AACTCTCAAAAGAAACAAGT+TGG	0.494236	2_4:-41154808	Msa0328550:CDS
AAGACACTTGTTTATTACAA+AGG	0.562015	2_4:-41154778	Msa0328550:CDS
AAGAGAAGTAATGCAAACTA+TGG	0.358125	2_4:-41153361	Msa0328550:CDS
AAGATCATTTCTTCCTAGCA+AGG	0.548567	2_4:-41154944	Msa0328550:CDS
AATCCAGGTGGAAGAGTCAT+TGG	0.438753	2_4:+41155222	None:intergenic
AATGTACCTGGTAAATCCCA+AGG	0.593393	2_4:+41155087	None:intergenic
AATGTATAACTGACTTATTT+AGG	0.328652	2_4:+41150113	None:intergenic
ACAAAGGTAGAGCACCTCAT+GGG	0.636035	2_4:-41154762	Msa0328550:CDS
ACAAATAGAGCAACAAGAGG+TGG	0.614366	2_4:-41154865	Msa0328550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTCTATAAATGTGAACCT+TGG	-	chr2_4:41148303-41148322	Msa0328550:three_prime_UTR	30.0%
TCTCTATAAATGTGAACCTT+GGG	-	chr2_4:41148304-41148323	Msa0328550:three_prime_UTR	30.0%
TTTGGATACTTTCTATCTCT+TGG	+	chr2_4:41148512-41148531	None:intergenic	30.0%
AACTCTCAAAAGAAACAAGT+TGG	-	chr2_4:41148599-41148618	Msa0328550:intron	30.0%
ATATTCATGCATAACCCAAT+TGG	+	chr2_4:41148678-41148697	None:intergenic	30.0%
CTTAATATGTACCTTCAAAG+AGG	+	chr2_4:41148735-41148754	None:intergenic	30.0%
TAAGAAGGAATATGTTAGCT+TGG	-	chr2_4:41148894-41148913	Msa0328550:intron	30.0%
CATTAACTCCTAATTAGAAG+GGG	+	chr2_4:41149187-41149206	None:intergenic	30.0%
TTAAAGCAAGCACTAAACAA+CGG	+	chr2_4:41149406-41149425	None:intergenic	30.0%
ACAAGATTGGAAAAGGAAAA+GGG	+	chr2_4:41149708-41149727	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	41148179	41155250	41148179	ID=Msa0328550;Name=Msa0328550
chr2_4	mRNA	41148179	41155250	41148179	ID=Msa0328550-mRNA-1;Parent=Msa0328550;Name=Msa0328550-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0\|0.62\|0.66\|1\|0.37\|0.33\|9\|758\|254
chr2_4	exon	41148179	41148449	41148179	ID=Msa0328550-mRNA-1:exon:12664;Parent=Msa0328550-mRNA-1
chr2_4	exon	41149035	41149181	41149035	ID=Msa0328550-mRNA-1:exon:12663;Parent=Msa0328550-mRNA-1
chr2_4	exon	41149349	41149417	41149349	ID=Msa0328550-mRNA-1:exon:12662;Parent=Msa0328550-mRNA-1
chr2_4	exon	41149854	41149908	41149854	ID=Msa0328550-mRNA-1:exon:12661;Parent=Msa0328550-mRNA-1
chr2_4	exon	41150068	41150247	41150068	ID=Msa0328550-mRNA-1:exon:12660;Parent=Msa0328550-mRNA-1
chr2_4	exon	41150727	41150768	41150727	ID=Msa0328550-mRNA-1:exon:12659;Parent=Msa0328550-mRNA-1
chr2_4	exon	41153155	41153535	41153155	ID=Msa0328550-mRNA-1:exon:12658;Parent=Msa0328550-mRNA-1
chr2_4	exon	41154747	41154967	41154747	ID=Msa0328550-mRNA-1:exon:12657;Parent=Msa0328550-mRNA-1
chr2_4	exon	41155094	41155250	41155094	ID=Msa0328550-mRNA-1:exon:12656;Parent=Msa0328550-mRNA-1
chr2_4	CDS	41155094	41155250	41155094	ID=Msa0328550-mRNA-1:cds;Parent=Msa0328550-mRNA-1
chr2_4	CDS	41154747	41154967	41154747	ID=Msa0328550-mRNA-1:cds;Parent=Msa0328550-mRNA-1
chr2_4	CDS	41153155	41153535	41153155	ID=Msa0328550-mRNA-1:cds;Parent=Msa0328550-mRNA-1
chr2_4	CDS	41150763	41150768	41150763	ID=Msa0328550-mRNA-1:cds;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41150727	41150762	41150727	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41150068	41150247	41150068	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41149854	41149908	41149854	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41149349	41149417	41149349	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41149035	41149181	41149035	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1
chr2_4	three_prime_UTR	41148179	41148449	41148179	ID=Msa0328550-mRNA-1:three_prime_utr;Parent=Msa0328550-mRNA-1

Gene Sequence

>Msa0328550

ATGGCTCCAATGACTCTTCCACCTGGATTCAGGTTCCACCCTACAGATGAAGAACTTGTTGCTTACTACTTAGAAAGAAAAATAACAGGAAGAACTATAGAGCTTGATATCATTGCAGAAGTTGATCTCTATAAATGTGAACCTTGGGATTTACCAGAAAGATCATTTCTTCCTAGCAAGGATATGGAGTGGTATTTTTATAGTCCAAGAGATAGAAAGTATCCAAATGGGTCAAGAACAAATAGAGCAACAAGAGGTGGTTATTGGAAAGCAACTGGAAAAGATAGGGCAGTGAACTCTCAAAAGAAACAAGTTGGAATGAAGAAGACACTTGTTTATTACAAAGGTAGAGCACCTCATGGGATTAGAACCAATTGGGATTCCTATTCATTGTGTCGAATTTTCAAGAAGACAATTCAAATTCCAACAAAAAATAATGAAGAGGAACAAGTTAAGGATACAAAGAAGGAAATGATGGTCTTAGAAGAACAAGTGCAAGGGGAAGACTCAAGTGGTGGCACAGAGATTTCAAGAGAAGTAATGCAAACTATGGATGAAAAAATTCTTAATCAAGATAAACATCCAAAATTTCCATGTGATGCTTCTTCATCTGAGGTCACTCAAGGAACATGTACACCAACAGATACATGTAATAATATACCCACAGAGGATTTTCATGCACAATTTGCCTATGAAGAAGAAAATAGTTCAGCTATTTCTTATCCAATGGGAATAAGCTACCCTTCAAATTTATTTCAGGTGTAA

Protein sequence

>Msa0328550

MAPMTLPPGFRFHPTDEELVAYYLERKITGRTIELDIIAEVDLYKCEPWDLPERSFLPSKDMEWYFYSPRDRKYPNGSRTNRATRGGYWKATGKDRAVNSQKKQVGMKKTLVYYKGRAPHGIRTNWDSYSLCRIFKKTIQIPTKNNEEEQVKDTKKEMMVLEEQVQGEDSSGGTEISREVMQTMDEKILNQDKHPKFPCDASSSEVTQGTCTPTDTCNNIPTEDFHAQFAYEEENSSAISYPMGISYPSNLFQV*