AlfalfaGEDB | Msa0424050

M. sativa cultivar zhongmu-4 / Msa0424050

KEGG: K03875
GO: GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010286 GO:0010311 GO:0010498 GO:0016567 GO:0016740 GO:0019005 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0031146 GO:0031461 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048518 GO:0048519 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048581 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051603 GO:0051716 GO:0051781 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901332 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905392 GO:1905393 GO:1990234 GO:2000023 GO:2000026 GO:2000069 GO:2000280
PFAM: PF12937.10

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424050	XP_013461356.1	96.703	364	12	0	1	364	1	364	0.0	716

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424050	sp\|Q9LPL4\|SKP2A_ARATH	72.191	356	87	3	21	364	1	356	0.0	512

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424050	B7FL57	96.703	364	12	0	1	364	1	364	0.0	716

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000910	Msa0424050	0.814961	1.180181e-51	-8.615850e-47
Msa0036970	Msa0424050	0.807220	5.622194e-50	-8.615850e-47
Msa0126790	Msa0424050	0.826475	2.658919e-54	-8.615850e-47
Msa0171870	Msa0424050	0.814336	1.622810e-51	-8.615850e-47
Msa0217670	Msa0424050	0.806243	9.043910e-50	-8.615850e-47
Msa0236770	Msa0424050	0.802399	5.715498e-49	-8.615850e-47
Msa0395980	Msa0424050	0.807455	5.011628e-50	-8.615850e-47
Msa0424050	Msa0430520	0.806944	6.431155e-50	-8.615850e-47
Msa0424050	Msa0430700	0.800380	1.481050e-48	-8.615850e-47
Msa0424050	Msa0440510	0.801352	9.375982e-49	-8.615850e-47
Msa0424050	Msa0476010	0.803787	2.951150e-49	-8.615850e-47
Msa0424050	Msa0600450	0.809726	1.640718e-50	-8.615850e-47
Msa0424050	Msa0640940	0.812762	3.602045e-51	-8.615850e-47
Msa0424050	Msa0645530	0.807981	3.876106e-50	-8.615850e-47
Msa0424050	Msa0679360	0.806364	8.528640e-50	-8.615850e-47
Msa0424050	Msa0685090	0.802175	6.356345e-49	-8.615850e-47
Msa0424050	Msa0789560	0.826474	2.659412e-54	-8.615850e-47
Msa0424050	Msa0805420	0.810883	9.236367e-51	-8.615850e-47
Msa0424050	Msa0842770	0.813187	2.905616e-51	-8.615850e-47
Msa0424050	Msa0982440	0.821904	3.150534e-53	-8.615850e-47
Msa0424050	Msa1133590	0.811051	8.492980e-51	-8.615850e-47
Msa0424050	Msa1205590	0.819595	1.069245e-52	-8.615850e-47
Msa0424050	Msa1206100	0.841617	4.262741e-58	-8.615850e-47
Msa0424050	Msa1329550	0.842705	2.196288e-58	-8.615850e-47
Msa0424050	Msa1342230	0.814672	1.367334e-51	-8.615850e-47
Msa0424050	Msa1373850	0.815981	6.997364e-52	-8.615850e-47
Msa0424050	Msa1391720	0.801793	7.614402e-49	-8.615850e-47
Msa0424050	Msa1426910	0.801481	8.824714e-49	-8.615850e-47
Msa0424050	Msa1433700	0.808675	2.756937e-50	-8.615850e-47
Msa0424050	Msa1440190	0.801209	1.003243e-48	-8.615850e-47
Msa0304840	Msa0424050	0.801225	9.955334e-49	-8.615850e-47
Msa0341230	Msa0424050	0.807185	5.719730e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424050	MtrunA17_Chr3g0125671	96.703	364	12	0	1	364	1	364	0.0	716
Msa0424050	MtrunA17_Chr1g0159531	82.407	324	53	2	35	358	23	342	0.0	548
Msa0424050	MtrunA17_Chr1g0212071	27.523	327	203	8	47	365	25	325	7.67e-23	99.8
Msa0424050	MtrunA17_Chr1g0212071	30.415	217	133	5	114	328	146	346	1.01e-21	96.7
Msa0424050	MtrunA17_Chr4g0004901	33.735	166	107	2	117	282	395	557	1.88e-21	97.1
Msa0424050	MtrunA17_Chr4g0004901	32.500	160	104	4	123	282	453	608	3.23e-14	74.7
Msa0424050	MtrunA17_Chr4g0004901	32.624	141	92	3	115	255	495	632	5.70e-11	64.7
Msa0424050	MtrunA17_Chr2g0286991	29.767	215	134	2	114	328	306	503	1.04e-19	91.7
Msa0424050	MtrunA17_Chr2g0286991	29.304	273	132	8	138	373	124	372	5.09e-19	89.4
Msa0424050	MtrunA17_Chr2g0286991	28.431	204	129	3	123	326	393	579	1.10e-14	76.3
Msa0424050	MtrunA17_Chr2g0286991	25.769	260	154	7	113	372	229	449	1.20e-13	72.8
Msa0424050	MtrunA17_Chr2g0286991	23.944	213	135	3	77	264	374	584	3.00e-11	65.5
Msa0424050	MtrunA17_Chr1g0176031	34.320	169	107	4	115	282	174	339	2.41e-17	82.8
Msa0424050	MtrunA17_Chr1g0176031	29.231	195	111	4	114	283	121	313	8.76e-16	78.2
Msa0424050	MtrunA17_Chr1g0176031	26.556	241	148	6	133	356	84	312	4.61e-12	67.0
Msa0424050	MtrunA17_Chr4g0063841	30.412	194	109	2	115	282	193	386	2.88e-17	84.3
Msa0424050	MtrunA17_Chr7g0272131	34.437	151	97	2	127	277	387	535	4.97e-17	83.6
Msa0424050	MtrunA17_Chr7g0272131	34.731	167	106	3	117	283	403	566	1.43e-13	72.8
Msa0424050	MtrunA17_Chr7g0272131	26.766	269	161	8	115	376	273	512	3.94e-12	68.2
Msa0424050	MtrunA17_Chr1g0149351	25.589	297	160	6	76	313	161	455	3.99e-16	80.5
Msa0424050	MtrunA17_Chr1g0149351	25.097	259	175	4	123	376	184	428	3.69e-13	71.2
Msa0424050	MtrunA17_Chr1g0149351	28.324	173	119	5	113	282	464	634	6.37e-11	64.3
Msa0424050	MtrunA17_Chr3g0137771	25.926	216	107	3	111	273	196	411	7.93e-15	76.6
Msa0424050	MtrunA17_Chr3g0137771	31.868	91	62	0	192	282	172	262	9.36e-11	63.9
Msa0424050	MtrunA17_Chr4g0070541	31.892	185	121	4	92	274	468	649	1.03e-14	76.3
Msa0424050	MtrunA17_Chr4g0073771	23.077	286	181	7	31	278	1	285	2.11e-14	73.6
Msa0424050	MtrunA17_Chr8g0347711	24.161	298	156	7	96	346	83	357	6.49e-13	70.1
Msa0424050	MtrunA17_Chr1g0152951	32.086	187	120	5	77	261	257	438	8.39e-13	70.1
Msa0424050	MtrunA17_Chr4g0070521	29.952	207	130	5	92	286	99	302	9.24e-13	68.9
Msa0424050	MtrunA17_Chr4g0017381	29.146	199	126	6	69	264	113	299	2.45e-12	67.8
Msa0424050	MtrunA17_Chr4g0008501	27.547	265	168	8	76	324	302	558	9.73e-12	67.0
Msa0424050	MtrunA17_Chr2g0300611	28.750	160	111	2	128	286	509	666	2.26e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424050	AT1G21410.2	72.191	356	87	3	21	364	1	356	0.0	512
Msa0424050	AT1G21410.1	72.191	356	87	3	21	364	1	356	0.0	512
Msa0424050	AT1G77000.2	69.553	358	96	3	10	364	9	356	1.40e-178	503
Msa0424050	AT1G77000.1	69.553	358	96	3	10	364	9	356	1.40e-178	503
Msa0424050	AT1G77000.3	68.908	238	64	1	10	247	9	236	7.47e-114	334
Msa0424050	AT1G77000.3	28.191	188	114	3	149	330	54	226	2.53e-12	67.0
Msa0424050	AT1G77000.4	68.908	238	64	1	10	247	9	236	7.47e-114	334
Msa0424050	AT1G77000.4	28.191	188	114	3	149	330	54	226	2.53e-12	67.0
Msa0424050	AT5G23340.1	30.292	274	167	7	114	371	136	401	1.32e-25	108
Msa0424050	AT5G23340.1	30.928	194	115	3	166	347	84	270	1.77e-18	87.4
Msa0424050	AT4G15475.1	29.870	231	141	5	98	328	372	581	2.64e-21	97.1
Msa0424050	AT4G15475.1	28.372	215	137	3	114	328	306	503	2.97e-20	93.6
Msa0424050	AT4G15475.1	27.670	206	146	3	77	282	193	395	7.42e-16	80.1
Msa0424050	AT4G15475.1	28.455	246	130	7	137	345	123	359	1.63e-14	75.9
Msa0424050	AT4G15475.1	24.766	214	132	4	77	264	374	584	3.71e-12	68.6
Msa0424050	AT3G58530.1	28.085	235	153	6	114	336	123	353	5.01e-19	88.2
Msa0424050	AT3G58530.1	25.962	208	134	4	141	332	94	297	2.94e-14	74.3
Msa0424050	AT5G01720.1	28.696	230	137	3	77	282	280	506	3.54e-18	87.4
Msa0424050	AT5G01720.1	26.848	257	136	7	113	342	83	314	2.80e-11	65.9
Msa0424050	AT5G27920.1	30.435	161	110	2	122	282	345	503	2.12e-15	79.0
Msa0424050	AT1G47056.1	33.537	164	103	4	111	272	267	426	5.62e-14	74.3
Msa0424050	AT5G25350.1	28.000	200	117	2	113	285	178	377	2.88e-13	72.0
Msa0424050	AT5G25350.1	28.384	229	142	6	130	341	143	366	6.75e-13	70.9
Msa0424050	AT2G25490.1	24.675	308	179	9	115	377	190	489	3.88e-13	71.6
Msa0424050	AT2G25490.1	33.766	154	93	4	135	280	124	276	6.37e-12	67.8
Msa0424050	AT2G25490.1	37.079	89	56	0	195	283	165	253	9.06e-11	64.3
Msa0424050	AT4G07400.1	31.515	165	108	3	122	284	310	471	4.55e-12	68.2
Msa0424050	AT2G06040.1	27.184	206	145	4	71	274	532	734	1.19e-11	67.4
Msa0424050	AT5G67140.1	25.862	232	113	9	42	267	10	188	1.34e-11	64.7
Msa0424050	AT5G57900.1	28.387	155	91	3	117	253	101	253	3.75e-11	64.3

Find 101 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATTAAAGGCAAAGAATT+TGG	0.188931	3_2:+74248305	Msa0424050:five_prime_UTR
TTGAACATTCGTGACTAAAT+TGG	0.199905	3_2:-74252422	None:intergenic
AAATTCTTTGCCTTTAATTT+AGG	0.201568	3_2:-74248303	None:intergenic
CTACAAAGAACCTAAATTAA+AGG	0.269996	3_2:+74248293	Msa0424050:five_prime_UTR
CTCACCAGAGGTTGCTAATC+AGG	0.282570	3_2:+74252396	Msa0424050:CDS
AATGCTCTGGCCTACCTTGC+TGG	0.303145	3_2:+74250440	Msa0424050:CDS
CAGACGGTGATTCGAGCTTC+TGG	0.354879	3_2:+74249151	Msa0424050:CDS
ATTTGGCTTTATCTTCTAAA+CGG	0.360436	3_2:+74248322	Msa0424050:five_prime_UTR
TTGCTCGTTTATCACTTTCA+TGG	0.360596	3_2:+74249212	Msa0424050:CDS
ATCTGCATCAAAAGCTCAAC+AGG	0.369088	3_2:-74249109	None:intergenic
GAGAATCTTATGATGGTTGC+TGG	0.376471	3_2:+74249031	Msa0424050:CDS
CCTGCTCTCCACACATGCTC+AGG	0.394593	3_2:+74251166	Msa0424050:CDS
CTGAATGAATAACAGGCTAT+TGG	0.394963	3_2:+74250616	Msa0424050:intron
CAGTTGCAGTCTTTGAACCT+TGG	0.398849	3_2:+74250652	Msa0424050:CDS
AAATGGAGAACACGATTCAT+TGG	0.398862	3_2:-74252367	None:intergenic
AGCTTCTGATACTGCACTTC+AGG	0.401548	3_2:+74250511	Msa0424050:CDS
ACTCACTCCTTCAGCTGTTC+AGG	0.403041	3_2:+74251126	Msa0424050:CDS
TGCCCTCACCTGAGGTCCCT+TGG	0.403174	3_2:+74250962	Msa0424050:CDS
TTCGAGGAAGTGCCCTTTGA+AGG	0.410954	3_2:+74251268	Msa0424050:CDS
TACTATCACTTTGCTTGTAA+AGG	0.414014	3_2:-74252490	None:intergenic
TGGAGAGATTCTATTTACTT+TGG	0.419180	3_2:+74249187	Msa0424050:CDS
CACAAAGCAAAGTGAAGAAC+AGG	0.424932	3_2:+74251032	Msa0424050:CDS
TGTGGCTGTGTCTATATAAC+AGG	0.428750	3_2:+74250751	Msa0424050:CDS
CTTCTGGTGTTTGTCGTGGT+TGG	0.432543	3_2:+74249167	Msa0424050:CDS
TTTGGCTTTATCTTCTAAAC+GGG	0.438350	3_2:+74248323	Msa0424050:five_prime_UTR
CAAACTTGGGAACAAGAGAT+AGG	0.439501	3_2:-74250211	None:intergenic
ACACGATTCATTGGGTGGAT+TGG	0.441007	3_2:-74252358	None:intergenic
CAACTGGACGACAATGTTGT+TGG	0.451784	3_2:+74250272	Msa0424050:CDS
CCTGAGCATGTGTGGAGAGC+AGG	0.456485	3_2:-74251166	None:intergenic
AGACAATGCCATGTATTCAT+TGG	0.463323	3_2:+74251009	Msa0424050:CDS
TGCTGAGGTCAAGAATCTGC+AGG	0.470249	3_2:-74250319	None:intergenic
TCGTCAAGATAAGCCACAAC+TGG	0.470692	3_2:+74250256	Msa0424050:CDS
TTGGTGAAGGGAAACTGAAA+TGG	0.472951	3_2:+74248964	Msa0424050:exon
AGCATTTAGTGACAATGCTC+TGG	0.474697	3_2:+74250427	Msa0424050:CDS
TCACTGTATGCTATTGCTCA+TGG	0.475331	3_2:+74250365	Msa0424050:CDS
CACTTCTCCGAGCCCTTCAA+AGG	0.480132	3_2:-74251281	None:intergenic
TAATAAACCACATGACTTCG+AGG	0.483879	3_2:+74251252	Msa0424050:CDS
GATGCAGATTCTGTCTCTTG+TGG	0.484308	3_2:+74249123	Msa0424050:CDS
TGTGACAAAGTTGGTGATGT+TGG	0.489189	3_2:+74250679	Msa0424050:CDS
AAATCAACTGTTCTTAGATC+AGG	0.489805	3_2:-74250727	None:intergenic
TGCAGTCTTTGAACCTTGGA+TGG	0.491087	3_2:+74250656	Msa0424050:CDS
AAGCGGCTCATGAATGTAGT+AGG	0.493290	3_2:+74252455	Msa0424050:CDS
AGTACAGTCCAAGGGACCTC+AGG	0.495892	3_2:-74250970	None:intergenic
AGATGATAGTGTGATTGCTT+TGG	0.499364	3_2:+74250931	Msa0424050:intron
ATGGTTGCTGGTGGTGAGAA+AGG	0.513155	3_2:+74249043	Msa0424050:CDS
AATGGAGAACACGATTCATT+GGG	0.519569	3_2:-74252366	None:intergenic
GGAACAAGCTTTGCAAAACA+AGG	0.520896	3_2:-74252337	None:intergenic
AGTGTGATTGCTTTGGCAAA+CGG	0.522528	3_2:+74250938	Msa0424050:CDS
GCAAAGTGAAGAACAGGATG+TGG	0.523649	3_2:+74251038	Msa0424050:CDS
GAAGCCTGATTAGCAACCTC+TGG	0.523928	3_2:-74252400	None:intergenic
GAAATGATGAAGATGGACTA+AGG	0.526857	3_2:+74251077	Msa0424050:CDS
CACTCCTTCAGCTGTTCAGG+CGG	0.526906	3_2:+74251129	Msa0424050:CDS
GAAGGGAAACTGAAATGGTT+GGG	0.529142	3_2:+74248969	Msa0424050:exon
CAACTTTGTCACACCATCCA+AGG	0.529983	3_2:-74250669	None:intergenic
CTGCTCTCCACACATGCTCA+GGG	0.533137	3_2:+74251167	Msa0424050:CDS
AAAGGAGGGAACATGAAGGT+TGG	0.533533	3_2:+74249061	Msa0424050:CDS
TCGAGGAAGTGCCCTTTGAA+GGG	0.540167	3_2:+74251269	Msa0424050:CDS
GTCCAAGGGACCTCAGGTGA+GGG	0.542888	3_2:-74250964	None:intergenic
GTCTCTTGTGGATGATCAGA+CGG	0.544667	3_2:+74249135	Msa0424050:CDS
GCTTTGTGCCAATGAATACA+TGG	0.550039	3_2:-74251017	None:intergenic
CGAGCTTCTGGTGTTTGTCG+TGG	0.551970	3_2:+74249163	Msa0424050:CDS
ACAACATTGTCGTCCAGTTG+TGG	0.553257	3_2:-74250269	None:intergenic
CTGAAAGTTCTAAATCTATG+TGG	0.556057	3_2:+74250476	Msa0424050:CDS
ACTTCTCCGAGCCCTTCAAA+GGG	0.556622	3_2:-74251280	None:intergenic
TCTACAGAAACCAGCAAGGT+AGG	0.559137	3_2:-74250450	None:intergenic
TGAAGGGAAACTGAAATGGT+TGG	0.561758	3_2:+74248968	Msa0424050:exon
GTTGCTGGTGGTGAGAAAGG+AGG	0.562599	3_2:+74249046	Msa0424050:CDS
AAAGGAGGAAATGATGAAGA+TGG	0.563283	3_2:+74251070	Msa0424050:CDS
CAAAGTGAAGAACAGGATGT+GGG	0.569923	3_2:+74251039	Msa0424050:CDS
GAAGGATAATTTAAAGACTG+AGG	0.570173	3_2:+74248991	Msa0424050:CDS
AAGGAGTGAGTGAAGTGCAC+TGG	0.570780	3_2:-74251114	None:intergenic
GGAAACTGAAATGGTTGGGA+AGG	0.572501	3_2:+74248973	Msa0424050:exon
GGCACTTCCTCGAAGTCATG+TGG	0.591410	3_2:-74251259	None:intergenic
GGAGAACACGATTCATTGGG+TGG	0.593210	3_2:-74252363	None:intergenic
AGGATGTGGGGATCTGTGAA+AGG	0.597813	3_2:+74251052	Msa0424050:CDS
TCTTGCAGAAGTACAGTCCA+AGG	0.602542	3_2:-74250979	None:intergenic
TTGCTGGTGGTGAGAAAGGA+GGG	0.605825	3_2:+74249047	Msa0424050:CDS
TGTTCAATTGAATGAGAAAG+CGG	0.608063	3_2:+74252438	Msa0424050:CDS
GGAGTGATGAGTCTAGCATA+TGG	0.608969	3_2:+74250700	Msa0424050:CDS
GAAGTGCCCTTTGAAGGGCT+CGG	0.610683	3_2:+74251274	Msa0424050:CDS
AGCACAAGCACAATGGACTT+CGG	0.611633	3_2:-74251227	None:intergenic
AGTCCAAGGGACCTCAGGTG+AGG	0.614611	3_2:-74250965	None:intergenic
CAAACGGATGCCCTCACCTG+AGG	0.618431	3_2:+74250954	Msa0424050:CDS
AATGCCTCCCTGAGCATGTG+TGG	0.621155	3_2:-74251174	None:intergenic
TGAGAAAGGAGGGAACATGA+AGG	0.625459	3_2:+74249057	Msa0424050:CDS
CTGACAAAACTAAACATCAG+TGG	0.630947	3_2:+74250398	Msa0424050:CDS
CTGATTAGCAACCTCTGGTG+AGG	0.635281	3_2:-74252395	None:intergenic
GTTGTGGCTTATCTTGACGA+AGG	0.635647	3_2:-74250253	None:intergenic
TGGTGTGATCACTGAATGGA+AGG	0.636114	3_2:+74249081	Msa0424050:CDS
AGGTTGGTGTGATCACTGAA+TGG	0.638216	3_2:+74249077	Msa0424050:CDS
TAACAAGAACATGAACAACT+TGG	0.639202	3_2:+74250187	Msa0424050:CDS
CAAACCGCCTGAACAGCTGA+AGG	0.645204	3_2:-74251133	None:intergenic
CTAAGAACAGTTGATTTGTG+TGG	0.647210	3_2:+74250733	Msa0424050:CDS
AGGCATTCACTCATCATGAG+TGG	0.653984	3_2:+74251190	Msa0424050:CDS
CTTGGATGGTGTGACAAAGT+TGG	0.660586	3_2:+74250670	Msa0424050:CDS
ATGTGGGGATCTGTGAAAGG+AGG	0.661005	3_2:+74251055	Msa0424050:CDS
TATTAATAGCACAAGCACAA+TGG	0.668791	3_2:-74251234	None:intergenic
CTCTCCACACATGCTCAGGG+AGG	0.670816	3_2:+74251170	Msa0424050:CDS
AAAGTGAAGAACAGGATGTG+GGG	0.676311	3_2:+74251040	Msa0424050:CDS
CTTGCAGAAGTACAGTCCAA+GGG	0.678760	3_2:-74250978	None:intergenic
AATCTTATGATGGTTGCTGG+TGG	0.732364	3_2:+74249034	Msa0424050:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTAATTGATTAAAGTTT+TGG	+	chr3_2:74248876-74248895	Msa0424050:intron	10.0%
!!!	TTTTTTGTTTAAATTGATTT+TGG	+	chr3_2:74249334-74249353	Msa0424050:intron	10.0%
!	GGGGGGGGGGGGGGGGGGGG+AGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!	GGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251322-74251341	None:intergenic	100.0%
!!	AAAACAAAAAATGATAGTTA+AGG	-	chr3_2:74248361-74248380	None:intergenic	15.0%
!!!	AAAACTTTATCATTTTTTTC+TGG	-	chr3_2:74248441-74248460	None:intergenic	15.0%
!!!	ATATTATTCATCAATTCTTT+TGG	+	chr3_2:74249391-74249410	Msa0424050:intron	15.0%
!!	AATATTACAGAAACTTAAAA+AGG	-	chr3_2:74251645-74251664	None:intergenic	15.0%
!!!	TTAAGTTTCTGTAATATTTT+TGG	+	chr3_2:74251647-74251666	Msa0424050:intron	15.0%
!!	AATTTAACCAAATTTAACAA+AGG	-	chr3_2:74251960-74251979	None:intergenic	15.0%
!!!	GTTCTTTGTAGTAATTTTTA+GGG	-	chr3_2:74248284-74248303	None:intergenic	20.0%
!!!	AAATTCTTTGCCTTTAATTT+AGG	-	chr3_2:74248306-74248325	None:intergenic	20.0%
!!	TAAATTAAAGGCAAAGAATT+TGG	+	chr3_2:74248305-74248324	Msa0424050:five_prime_UTR	20.0%
!!!	CACTTTTTTTTAATCAAACT+AGG	-	chr3_2:74248556-74248575	None:intergenic	20.0%
!!!	ATAAAGGGTCTATTTTTTTA+AGG	-	chr3_2:74248735-74248754	None:intergenic	20.0%
!!	GGGATAATTACAATTATAAT+TGG	-	chr3_2:74248787-74248806	None:intergenic	20.0%
!!!	TGAAACTATTTTTCTCAAAT+TGG	+	chr3_2:74249284-74249303	Msa0424050:intron	20.0%
!!	GATGAATAATATTGAAAACA+TGG	-	chr3_2:74249383-74249402	None:intergenic	20.0%
!!	ATTAAACTGTATATTCAAAC+AGG	+	chr3_2:74249741-74249760	Msa0424050:intron	20.0%
!!	TTAAACTGTATATTCAAACA+GGG	+	chr3_2:74249742-74249761	Msa0424050:intron	20.0%
!!	TAAGAAATTGAATTCAACAT+TGG	-	chr3_2:74250027-74250046	None:intergenic	20.0%
!!	TGTTCATAAAACTATAAAAG+TGG	+	chr3_2:74250090-74250109	Msa0424050:intron	20.0%
!!	CAATCATATTAAAACTAAGA+TGG	+	chr3_2:74250564-74250583	Msa0424050:intron	20.0%
!!	ATTGTTATATGTTTATAACG+AGG	+	chr3_2:74250888-74250907	Msa0424050:intron	20.0%
!!	TTGTTATATGTTTATAACGA+GGG	+	chr3_2:74250889-74250908	Msa0424050:intron	20.0%
!!	TTTAATTAATACCTCAACTA+TGG	+	chr3_2:74251453-74251472	Msa0424050:intron	20.0%
!!	ATAAATTTATACAGCTAAAC+AGG	-	chr3_2:74251517-74251536	None:intergenic	20.0%
!!	GTTTAGCTGTATAAATTTAT+TGG	+	chr3_2:74251517-74251536	Msa0424050:intron	20.0%
!!	TATAAATTTATTGGAAGACA+TGG	+	chr3_2:74251526-74251545	Msa0424050:intron	20.0%
!!	GTTTAATTGAGATTCAAATT+CGG	+	chr3_2:74251679-74251698	Msa0424050:intron	20.0%
!!	TTTAATTGAGATTCAAATTC+GGG	+	chr3_2:74251680-74251699	Msa0424050:intron	20.0%
!!!	CAATTTTCATTAACTATTTG+AGG	-	chr3_2:74251744-74251763	None:intergenic	20.0%
!!	AAATTGAACTCATTAACTAT+CGG	+	chr3_2:74251758-74251777	Msa0424050:intron	20.0%
!!!	TTAAGGAAGAAATTTTGTTT+CGG	+	chr3_2:74251800-74251819	Msa0424050:intron	20.0%
!!!	AGGATTCTAAAATAGAAAAT+TGG	-	chr3_2:74251886-74251905	None:intergenic	20.0%
!!!	TTCTTCACATTTTACAAAAA+CGG	+	chr3_2:74251984-74252003	Msa0424050:intron	20.0%
!!	CTTAGTACAATAATAAATCA+TGG	+	chr3_2:74252032-74252051	Msa0424050:intron	20.0%
!!	GGTTCTTTGTAGTAATTTTT+AGG	-	chr3_2:74248285-74248304	None:intergenic	25.0%
!	CTACAAAGAACCTAAATTAA+AGG	+	chr3_2:74248293-74248312	Msa0424050:five_prime_UTR	25.0%
!!!	ATTTGGCTTTATCTTCTAAA+CGG	+	chr3_2:74248322-74248341	Msa0424050:five_prime_UTR	25.0%
!	AAAAATGATAGTTAAGGAAG+TGG	-	chr3_2:74248355-74248374	None:intergenic	25.0%
!	AATCATCAAGAAATGAAAGT+GGG	-	chr3_2:74248477-74248496	None:intergenic	25.0%
!	AAATCATCAAGAAATGAAAG+TGG	-	chr3_2:74248478-74248497	None:intergenic	25.0%
!	ACAAAGCCAAAATATTAAGA+GGG	-	chr3_2:74248508-74248527	None:intergenic	25.0%
!	TGTAATTGAGAAATTTGTGA+TGG	+	chr3_2:74248685-74248704	Msa0424050:intron	25.0%
!	GTAATTGAGAAATTTGTGAT+GGG	+	chr3_2:74248686-74248705	Msa0424050:intron	25.0%
!!!	AAAGTTTTGGAATTTTGCTA+TGG	+	chr3_2:74248889-74248908	Msa0424050:intron	25.0%
!!!	GTTTTTTGAGAATCTTATGA+TGG	+	chr3_2:74249024-74249043	Msa0424050:CDS	25.0%
!!	TCAAATTGGTGTATTTTTCT+TGG	+	chr3_2:74249298-74249317	Msa0424050:intron	25.0%
!!!	ATGAATTGCTTACTTTTGAA+GGG	+	chr3_2:74249458-74249477	Msa0424050:intron	25.0%
!!!	ATATGTGAGTTTTATTGACA+TGG	-	chr3_2:74249510-74249529	None:intergenic	25.0%
!!	TTTGATGTTTTCAAAATGTG+TGG	+	chr3_2:74249530-74249549	Msa0424050:intron	25.0%
!	TAAAGTAAACAAAAGCTACA+GGG	-	chr3_2:74249847-74249866	None:intergenic	25.0%
!!	ATTATGTAATTCCTCTTTTC+AGG	+	chr3_2:74250162-74250181	Msa0424050:intron	25.0%
!	ATATGTTTGTGACTTTATGT+TGG	+	chr3_2:74251378-74251397	Msa0424050:intron	25.0%
!!	AAAGGTAGTAGTGATTTTAT+AGG	-	chr3_2:74251627-74251646	None:intergenic	25.0%
!	ATCACTTTGTTAACTATGAT+TGG	+	chr3_2:74251718-74251737	Msa0424050:intron	25.0%
!	AATTGAACTCATTAACTATC+GGG	+	chr3_2:74251759-74251778	Msa0424050:intron	25.0%
!	GAAACAAAATTTCTTCCTTA+AGG	-	chr3_2:74251801-74251820	None:intergenic	25.0%
!!!	TAAGGAAGAAATTTTGTTTC+GGG	+	chr3_2:74251801-74251820	Msa0424050:intron	25.0%
!	CTTGTTAACAAATGCATTTA+TGG	-	chr3_2:74251918-74251937	None:intergenic	25.0%
!!!	AATTGTTACTTTGTTATGAC+TGG	+	chr3_2:74252110-74252129	Msa0424050:intron	25.0%
!!!	GTAGTAATTTTTAGGGTGTT+TGG	-	chr3_2:74248277-74248296	None:intergenic	30.0%
!!	TTTGGCTTTATCTTCTAAAC+GGG	+	chr3_2:74248323-74248342	Msa0424050:five_prime_UTR	30.0%
	CACAAAGCCAAAATATTAAG+AGG	-	chr3_2:74248509-74248528	None:intergenic	30.0%
!!!	AGTTTTAAACACAGAACAAG+TGG	-	chr3_2:74248633-74248652	None:intergenic	30.0%
!!	CATCAGAATTCTTTGATTTG+TGG	+	chr3_2:74248843-74248862	Msa0424050:intron	30.0%
!!!	CTAGGAAAGTTGATGATTTT+TGG	+	chr3_2:74248945-74248964	Msa0424050:five_prime_UTR	30.0%
!!!	AAGTTGATGATTTTTGGTGA+AGG	+	chr3_2:74248951-74248970	Msa0424050:five_prime_UTR	30.0%
!!!	AGTTGATGATTTTTGGTGAA+GGG	+	chr3_2:74248952-74248971	Msa0424050:five_prime_UTR	30.0%
	GAAGGATAATTTAAAGACTG+AGG	+	chr3_2:74248991-74249010	Msa0424050:CDS	30.0%
	TGGAGAGATTCTATTTACTT+TGG	+	chr3_2:74249187-74249206	Msa0424050:CDS	30.0%
!!	GATGAATTGCTTACTTTTGA+AGG	+	chr3_2:74249457-74249476	Msa0424050:intron	30.0%
!!	TGAATTGCTTACTTTTGAAG+GGG	+	chr3_2:74249459-74249478	Msa0424050:intron	30.0%
!	TTAAGTTGTTCATCCAAACA+GGG	+	chr3_2:74249705-74249724	Msa0424050:intron	30.0%
	GTAAAGTAAACAAAAGCTAC+AGG	-	chr3_2:74249848-74249867	None:intergenic	30.0%
	CCAACTTCAGAAATTATAAG+AGG	-	chr3_2:74249904-74249923	None:intergenic	30.0%
!!	CCTCTTATAATTTCTGAAGT+TGG	+	chr3_2:74249901-74249920	Msa0424050:intron	30.0%
	GTTGACGTTATAATGTTTCT+TGG	+	chr3_2:74249982-74250001	Msa0424050:intron	30.0%
	ATTGAATTCAACATTGGAAC+CGG	-	chr3_2:74250021-74250040	None:intergenic	30.0%
	TAACAAGAACATGAACAACT+TGG	+	chr3_2:74250187-74250206	Msa0424050:CDS	30.0%
!!!	TGTTTAGTTTTGTCAGATCA+CGG	-	chr3_2:74250394-74250413	None:intergenic	30.0%
!!	CTGAAAGTTCTAAATCTATG+TGG	+	chr3_2:74250476-74250495	Msa0424050:CDS	30.0%
	TTCATTCAGTAATGTCTCAT+GGG	-	chr3_2:74250605-74250624	None:intergenic	30.0%
	ATTCATTCAGTAATGTCTCA+TGG	-	chr3_2:74250606-74250625	None:intergenic	30.0%
	GACATTACTGAATGAATAAC+AGG	+	chr3_2:74250609-74250628	Msa0424050:intron	30.0%
	AAATCAACTGTTCTTAGATC+AGG	-	chr3_2:74250730-74250749	None:intergenic	30.0%
	GTTTATAACGAGGGTAATTT+CGG	+	chr3_2:74250898-74250917	Msa0424050:intron	30.0%
	TATTAATAGCACAAGCACAA+TGG	-	chr3_2:74251237-74251256	None:intergenic	30.0%
	ATCTTAAAAGTCCATAGTTG+AGG	-	chr3_2:74251467-74251486	None:intergenic	30.0%
	AATTTAACAAAGGCCTCAAA+TGG	-	chr3_2:74251950-74251969	None:intergenic	30.0%
!	TTTGAGGCCTTTGTTAAATT+TGG	+	chr3_2:74251950-74251969	Msa0424050:intron	30.0%
	TAAATCATGGTTAACTCACA+TGG	+	chr3_2:74252045-74252064	Msa0424050:intron	30.0%
	AAATCATGGTTAACTCACAT+GGG	+	chr3_2:74252046-74252065	Msa0424050:intron	30.0%
	AATCACTAATGTTGAATGTC+TGG	-	chr3_2:74252155-74252174	None:intergenic	30.0%
	TCTTACTTTATTCCATGACT+TGG	+	chr3_2:74252236-74252255	Msa0424050:intron	30.0%
	TTGAACATTCGTGACTAAAT+TGG	-	chr3_2:74252425-74252444	None:intergenic	30.0%
	TGTTCAATTGAATGAGAAAG+CGG	+	chr3_2:74252438-74252457	Msa0424050:CDS	30.0%
!	CACTTGCCCTCTTAATATTT+TGG	+	chr3_2:74248499-74248518	Msa0424050:intron	35.0%
	TTGCTCGTTTATCACTTTCA+TGG	+	chr3_2:74249212-74249231	Msa0424050:CDS	35.0%
	AATATTGAAAACATGGCACC+AGG	-	chr3_2:74249376-74249395	None:intergenic	35.0%
	CAGAAAATGTATAGCTGAGA+AGG	+	chr3_2:74249570-74249589	Msa0424050:intron	35.0%
!	ATAGCTGAGAAGGATTTGTT+TGG	+	chr3_2:74249580-74249599	Msa0424050:intron	35.0%
!	TGAGAAGGATTTGTTTGGAT+TGG	+	chr3_2:74249585-74249604	Msa0424050:intron	35.0%
!	AGGATTTGTTTGGATTGGAT+TGG	+	chr3_2:74249590-74249609	Msa0424050:intron	35.0%
!	CTTAAGTTGTTCATCCAAAC+AGG	+	chr3_2:74249704-74249723	Msa0424050:intron	35.0%
	TTCCACAGCAAAAATACAAC+AGG	-	chr3_2:74249789-74249808	None:intergenic	35.0%
	AGATTTATTCCAGGAATGCA+GGG	-	chr3_2:74249931-74249950	None:intergenic	35.0%
	AAGACTTGCAGATTTATTCC+AGG	-	chr3_2:74249940-74249959	None:intergenic	35.0%
	AAAAGATGGGGGAAAATCAA+CGG	-	chr3_2:74250054-74250073	None:intergenic	35.0%
	TTCTTGTTACACCTGAAAAG+AGG	-	chr3_2:74250176-74250195	None:intergenic	35.0%
!	GTTTGCAGTTTTGCAAACTT+GGG	-	chr3_2:74250227-74250246	None:intergenic	35.0%
!	AGTTTGCAGTTTTGCAAACT+TGG	-	chr3_2:74250228-74250247	None:intergenic	35.0%
	CTGACAAAACTAAACATCAG+TGG	+	chr3_2:74250398-74250417	Msa0424050:CDS	35.0%
	ACTGCACTTCAGGTATTATA+TGG	+	chr3_2:74250521-74250540	Msa0424050:intron	35.0%
	TCATTCAGTAATGTCTCATG+GGG	-	chr3_2:74250604-74250623	None:intergenic	35.0%
	CTGAATGAATAACAGGCTAT+TGG	+	chr3_2:74250616-74250635	Msa0424050:intron	35.0%
!!	CTAAGAACAGTTGATTTGTG+TGG	+	chr3_2:74250733-74250752	Msa0424050:CDS	35.0%
!	AGATGATAGTGTGATTGCTT+TGG	+	chr3_2:74250931-74250950	Msa0424050:intron	35.0%
	AGACAATGCCATGTATTCAT+TGG	+	chr3_2:74251009-74251028	Msa0424050:CDS	35.0%
	AAAGGAGGAAATGATGAAGA+TGG	+	chr3_2:74251070-74251089	Msa0424050:CDS	35.0%
!!	GAAATGATGAAGATGGACTA+AGG	+	chr3_2:74251077-74251096	Msa0424050:CDS	35.0%
	TAATAAACCACATGACTTCG+AGG	+	chr3_2:74251252-74251271	Msa0424050:CDS	35.0%
	TCACAAACATATCTACACCA+GGG	-	chr3_2:74251370-74251389	None:intergenic	35.0%
	AGTATGAGCACAACAACAAA+TGG	-	chr3_2:74251427-74251446	None:intergenic	35.0%
	TTTATTGGAAGACATGGACT+TGG	+	chr3_2:74251532-74251551	Msa0424050:intron	35.0%
	GACTTCTTCTAAACGTTGTT+TGG	+	chr3_2:74251554-74251573	Msa0424050:intron	35.0%
!!!	TCTAAACGTTGTTTGGCTTT+TGG	+	chr3_2:74251561-74251580	Msa0424050:intron	35.0%
!!!	TTTGGCTTTTGGAAGAATGT+GGG	+	chr3_2:74251572-74251591	Msa0424050:intron	35.0%
	AATGCTAACAAGTGTCCTTA+AGG	+	chr3_2:74251783-74251802	Msa0424050:intron	35.0%
	AATCATGGTTAACTCACATG+GGG	+	chr3_2:74252047-74252066	Msa0424050:intron	35.0%
	CTAATGTTGAATGTCTGGAA+GGG	-	chr3_2:74252150-74252169	None:intergenic	35.0%
	ACTAATGTTGAATGTCTGGA+AGG	-	chr3_2:74252151-74252170	None:intergenic	35.0%
	TTCCACCTAGCTAAGAAATT+CGG	+	chr3_2:74252295-74252314	Msa0424050:intron	35.0%
	AATGGAGAACACGATTCATT+GGG	-	chr3_2:74252369-74252388	None:intergenic	35.0%
	AAATGGAGAACACGATTCAT+TGG	-	chr3_2:74252370-74252389	None:intergenic	35.0%
	GGCATGCACATACACAAAAT+AGG	-	chr3_2:74248535-74248554	None:intergenic	40.0%
	GCAATTTGCAGAAAGCAACA+TGG	-	chr3_2:74248816-74248835	None:intergenic	40.0%
!	CTTATCCTTTTGTGACAGCT+AGG	+	chr3_2:74248927-74248946	Msa0424050:intron	40.0%
	ACTTTCCTAGCTGTCACAAA+AGG	-	chr3_2:74248935-74248954	None:intergenic	40.0%
	TTGGTGAAGGGAAACTGAAA+TGG	+	chr3_2:74248964-74248983	Msa0424050:exon	40.0%
	TGAAGGGAAACTGAAATGGT+TGG	+	chr3_2:74248968-74248987	Msa0424050:exon	40.0%
	GAAGGGAAACTGAAATGGTT+GGG	+	chr3_2:74248969-74248988	Msa0424050:exon	40.0%
	GAGAATCTTATGATGGTTGC+TGG	+	chr3_2:74249031-74249050	Msa0424050:CDS	40.0%
	AATCTTATGATGGTTGCTGG+TGG	+	chr3_2:74249034-74249053	Msa0424050:CDS	40.0%
	ATCTGCATCAAAAGCTCAAC+AGG	-	chr3_2:74249112-74249131	None:intergenic	40.0%
!!	GTCCTGTTGTATTTTTGCTG+TGG	+	chr3_2:74249784-74249803	Msa0424050:intron	40.0%
	GATTTATTCCAGGAATGCAG+GGG	-	chr3_2:74249930-74249949	None:intergenic	40.0%
	CAGATTTATTCCAGGAATGC+AGG	-	chr3_2:74249932-74249951	None:intergenic	40.0%
	TGAGATGAGTGCAAAAGATG+GGG	-	chr3_2:74250066-74250085	None:intergenic	40.0%
	CTGAGATGAGTGCAAAAGAT+GGG	-	chr3_2:74250067-74250086	None:intergenic	40.0%
!!	ATTGTGAAGTGCATTGACTG+TGG	+	chr3_2:74250129-74250148	Msa0424050:intron	40.0%
	CAAACTTGGGAACAAGAGAT+AGG	-	chr3_2:74250214-74250233	None:intergenic	40.0%
!	TGAGCTTGAAACTTTTGCTG+AGG	-	chr3_2:74250337-74250356	None:intergenic	40.0%
	TCACTGTATGCTATTGCTCA+TGG	+	chr3_2:74250365-74250384	Msa0424050:CDS	40.0%
	AGCATTTAGTGACAATGCTC+TGG	+	chr3_2:74250427-74250446	Msa0424050:CDS	40.0%
!	GTTTTCTACAGAAACCAGCA+AGG	-	chr3_2:74250457-74250476	None:intergenic	40.0%
	CATTCAGTAATGTCTCATGG+GGG	-	chr3_2:74250603-74250622	None:intergenic	40.0%
!!	TGTGACAAAGTTGGTGATGT+TGG	+	chr3_2:74250679-74250698	Msa0424050:CDS	40.0%
	TGTGGCTGTGTCTATATAAC+AGG	+	chr3_2:74250751-74250770	Msa0424050:CDS	40.0%
	ATATGTTGTGCTCTGTAGCA+CGG	+	chr3_2:74250815-74250834	Msa0424050:intron	40.0%
	AGTGTGATTGCTTTGGCAAA+CGG	+	chr3_2:74250938-74250957	Msa0424050:CDS	40.0%
	GCTTTGTGCCAATGAATACA+TGG	-	chr3_2:74251020-74251039	None:intergenic	40.0%
	CACAAAGCAAAGTGAAGAAC+AGG	+	chr3_2:74251032-74251051	Msa0424050:CDS	40.0%
	CAAAGTGAAGAACAGGATGT+GGG	+	chr3_2:74251039-74251058	Msa0424050:CDS	40.0%
	AAAGTGAAGAACAGGATGTG+GGG	+	chr3_2:74251040-74251059	Msa0424050:CDS	40.0%
	CACAAACATATCTACACCAG+GGG	-	chr3_2:74251369-74251388	None:intergenic	40.0%
	GTCACAAACATATCTACACC+AGG	-	chr3_2:74251371-74251390	None:intergenic	40.0%
!!!	GTTTGGCTTTTGGAAGAATG+TGG	+	chr3_2:74251571-74251590	Msa0424050:intron	40.0%
!	GAAGAATGTGGGTTGAACAA+TGG	+	chr3_2:74251583-74251602	Msa0424050:intron	40.0%
	GGGTTGAACAATGGTTAAAC+AGG	+	chr3_2:74251592-74251611	Msa0424050:intron	40.0%
	TGCATTTATGGCACTCGTTA+AGG	-	chr3_2:74251906-74251925	None:intergenic	40.0%
	ACAAAGGCCTCAAATGGTTA+GGG	-	chr3_2:74251944-74251963	None:intergenic	40.0%
	AACAAAGGCCTCAAATGGTT+AGG	-	chr3_2:74251945-74251964	None:intergenic	40.0%
	ATCATGGTTAACTCACATGG+GGG	+	chr3_2:74252048-74252067	Msa0424050:intron	40.0%
	GTACTTAAGTGTCCTACTCT+AGG	-	chr3_2:74252077-74252096	None:intergenic	40.0%
	GTTAGGAACATACCAAGTCA+TGG	-	chr3_2:74252251-74252270	None:intergenic	40.0%
	AGCGTTCTGTTCACTATGTT+AGG	-	chr3_2:74252268-74252287	None:intergenic	40.0%
	AACCGAATTTCTTAGCTAGG+TGG	-	chr3_2:74252300-74252319	None:intergenic	40.0%
	AGCAACCGAATTTCTTAGCT+AGG	-	chr3_2:74252303-74252322	None:intergenic	40.0%
	AGCTAAGAAATTCGGTTGCT+TGG	+	chr3_2:74252303-74252322	Msa0424050:intron	40.0%
	GGAACAAGCTTTGCAAAACA+AGG	-	chr3_2:74252340-74252359	None:intergenic	40.0%
	AGAAAGCAACATGGCTCATG+GGG	-	chr3_2:74248807-74248826	None:intergenic	45.0%
	CAGAAAGCAACATGGCTCAT+GGG	-	chr3_2:74248808-74248827	None:intergenic	45.0%
	GGAAACTGAAATGGTTGGGA+AGG	+	chr3_2:74248973-74248992	Msa0424050:exon	45.0%
	TGAGAAAGGAGGGAACATGA+AGG	+	chr3_2:74249057-74249076	Msa0424050:CDS	45.0%
	AAAGGAGGGAACATGAAGGT+TGG	+	chr3_2:74249061-74249080	Msa0424050:CDS	45.0%
!!	AGGTTGGTGTGATCACTGAA+TGG	+	chr3_2:74249077-74249096	Msa0424050:CDS	45.0%
	TGGTGTGATCACTGAATGGA+AGG	+	chr3_2:74249081-74249100	Msa0424050:CDS	45.0%
	GATGCAGATTCTGTCTCTTG+TGG	+	chr3_2:74249123-74249142	Msa0424050:CDS	45.0%
	GTCTCTTGTGGATGATCAGA+CGG	+	chr3_2:74249135-74249154	Msa0424050:CDS	45.0%
	GGTTCTTCAATTCGTGATCC+TGG	+	chr3_2:74249355-74249374	Msa0424050:intron	45.0%
	GCTTATGACTTAGCCCTGTT+TGG	-	chr3_2:74249721-74249740	None:intergenic	45.0%
	TCTTGGAGATCATAGCACAC+CGG	+	chr3_2:74249999-74250018	Msa0424050:intron	45.0%
	GAGATGAGTGCAAAAGATGG+GGG	-	chr3_2:74250065-74250084	None:intergenic	45.0%
	CCTGAGATGAGTGCAAAAGA+TGG	-	chr3_2:74250068-74250087	None:intergenic	45.0%
!	CCATCTTTTGCACTCATCTC+AGG	+	chr3_2:74250065-74250084	Msa0424050:intron	45.0%
	GTTGTGGCTTATCTTGACGA+AGG	-	chr3_2:74250256-74250275	None:intergenic	45.0%
	TCGTCAAGATAAGCCACAAC+TGG	+	chr3_2:74250256-74250275	Msa0424050:CDS	45.0%
	ACAACATTGTCGTCCAGTTG+TGG	-	chr3_2:74250272-74250291	None:intergenic	45.0%
	CAACTGGACGACAATGTTGT+TGG	+	chr3_2:74250272-74250291	Msa0424050:CDS	45.0%
	TCTACAGAAACCAGCAAGGT+AGG	-	chr3_2:74250453-74250472	None:intergenic	45.0%
	AGCTTCTGATACTGCACTTC+AGG	+	chr3_2:74250511-74250530	Msa0424050:CDS	45.0%
	CAGTTGCAGTCTTTGAACCT+TGG	+	chr3_2:74250652-74250671	Msa0424050:CDS	45.0%
	TGCAGTCTTTGAACCTTGGA+TGG	+	chr3_2:74250656-74250675	Msa0424050:CDS	45.0%
	CAACTTTGTCACACCATCCA+AGG	-	chr3_2:74250672-74250691	None:intergenic	45.0%
	CTTGGATGGTGTGACAAAGT+TGG	+	chr3_2:74250670-74250689	Msa0424050:CDS	45.0%
	GGAGTGATGAGTCTAGCATA+TGG	+	chr3_2:74250700-74250719	Msa0424050:CDS	45.0%
!	CTTGCAGAAGTACAGTCCAA+GGG	-	chr3_2:74250981-74251000	None:intergenic	45.0%
!	TCTTGCAGAAGTACAGTCCA+AGG	-	chr3_2:74250982-74251001	None:intergenic	45.0%
	GCAAAGTGAAGAACAGGATG+TGG	+	chr3_2:74251038-74251057	Msa0424050:CDS	45.0%
	AGGCATTCACTCATCATGAG+TGG	+	chr3_2:74251190-74251209	Msa0424050:CDS	45.0%
!	AGCACAAGCACAATGGACTT+CGG	-	chr3_2:74251230-74251249	None:intergenic	45.0%
	ATCTACACCAGGGGAAAGAA+GGG	-	chr3_2:74251360-74251379	None:intergenic	45.0%
	TATCTACACCAGGGGAAAGA+AGG	-	chr3_2:74251361-74251380	None:intergenic	45.0%
	AAAGGCCTCAAATGGTTAGG+GGG	-	chr3_2:74251942-74251961	None:intergenic	45.0%
	CAAAGGCCTCAAATGGTTAG+GGG	-	chr3_2:74251943-74251962	None:intergenic	45.0%
	ACACGATTCATTGGGTGGAT+TGG	-	chr3_2:74252361-74252380	None:intergenic	45.0%
	AAGCGGCTCATGAATGTAGT+AGG	+	chr3_2:74252455-74252474	Msa0424050:CDS	45.0%
!!	AAATAAAAAACAAAAATAAA+GGG	-	chr3_2:74248750-74248769	None:intergenic	5.0%
!!	AAAATAAAAAACAAAAATAA+AGG	-	chr3_2:74248751-74248770	None:intergenic	5.0%
	GCAGAAAGCAACATGGCTCA+TGG	-	chr3_2:74248809-74248828	None:intergenic	50.0%
!	ATGGTTGCTGGTGGTGAGAA+AGG	+	chr3_2:74249043-74249062	Msa0424050:CDS	50.0%
!	TTGCTGGTGGTGAGAAAGGA+GGG	+	chr3_2:74249047-74249066	Msa0424050:CDS	50.0%
	CTTCTGGTGTTTGTCGTGGT+TGG	+	chr3_2:74249167-74249186	Msa0424050:CDS	50.0%
	TGCTGAGGTCAAGAATCTGC+AGG	-	chr3_2:74250322-74250341	None:intergenic	50.0%
	AGGATGTGGGGATCTGTGAA+AGG	+	chr3_2:74251052-74251071	Msa0424050:CDS	50.0%
	ATGTGGGGATCTGTGAAAGG+AGG	+	chr3_2:74251055-74251074	Msa0424050:CDS	50.0%
	AAGGAGTGAGTGAAGTGCAC+TGG	-	chr3_2:74251117-74251136	None:intergenic	50.0%
	ACTCACTCCTTCAGCTGTTC+AGG	+	chr3_2:74251126-74251145	Msa0424050:CDS	50.0%
!!	TTCGAGGAAGTGCCCTTTGA+AGG	+	chr3_2:74251268-74251287	Msa0424050:CDS	50.0%
!!	TCGAGGAAGTGCCCTTTGAA+GGG	+	chr3_2:74251269-74251288	Msa0424050:CDS	50.0%
	ACTTCTCCGAGCCCTTCAAA+GGG	-	chr3_2:74251283-74251302	None:intergenic	50.0%
	TCTACACCAGGGGAAAGAAG+GGG	-	chr3_2:74251359-74251378	None:intergenic	50.0%
	CAAGACCCCCTAACCATTTG+AGG	+	chr3_2:74251934-74251953	Msa0424050:intron	50.0%
	ACATGGGGGTAACCTAGAGT+AGG	+	chr3_2:74252062-74252081	Msa0424050:intron	50.0%
!	GTTATGACTGGTCAAGCTCC+CGG	+	chr3_2:74252122-74252141	Msa0424050:intron	50.0%
!	TTATGACTGGTCAAGCTCCC+GGG	+	chr3_2:74252123-74252142	Msa0424050:intron	50.0%
	TTGAATGTCTGGAAGGGTCC+CGG	-	chr3_2:74252144-74252163	None:intergenic	50.0%
	GGAGAACACGATTCATTGGG+TGG	-	chr3_2:74252366-74252385	None:intergenic	50.0%
	CCTCTGGTGAGGAGCAAAAA+TGG	-	chr3_2:74252387-74252406	None:intergenic	50.0%
!!	CCATTTTTGCTCCTCACCAG+AGG	+	chr3_2:74252384-74252403	Msa0424050:CDS	50.0%
	CTGATTAGCAACCTCTGGTG+AGG	-	chr3_2:74252398-74252417	None:intergenic	50.0%
	CTCACCAGAGGTTGCTAATC+AGG	+	chr3_2:74252396-74252415	Msa0424050:CDS	50.0%
	GAAGCCTGATTAGCAACCTC+TGG	-	chr3_2:74252403-74252422	None:intergenic	50.0%
!	GTTGCTGGTGGTGAGAAAGG+AGG	+	chr3_2:74249046-74249065	Msa0424050:CDS	55.0%
	CAGACGGTGATTCGAGCTTC+TGG	+	chr3_2:74249151-74249170	Msa0424050:CDS	55.0%
	CGAGCTTCTGGTGTTTGTCG+TGG	+	chr3_2:74249163-74249182	Msa0424050:CDS	55.0%
!	GTTGGTTTCCCCTGCATTCC+TGG	+	chr3_2:74249919-74249938	Msa0424050:intron	55.0%
	AATGCTCTGGCCTACCTTGC+TGG	+	chr3_2:74250440-74250459	Msa0424050:CDS	55.0%
	TGTGCTCTGTAGCACGGACA+CGG	+	chr3_2:74250821-74250840	Msa0424050:intron	55.0%
	AGTACAGTCCAAGGGACCTC+AGG	-	chr3_2:74250973-74250992	None:intergenic	55.0%
	CACTCCTTCAGCTGTTCAGG+CGG	+	chr3_2:74251129-74251148	Msa0424050:CDS	55.0%
	CAAACCGCCTGAACAGCTGA+AGG	-	chr3_2:74251136-74251155	None:intergenic	55.0%
	CTGCTCTCCACACATGCTCA+GGG	+	chr3_2:74251167-74251186	Msa0424050:CDS	55.0%
	AATGCCTCCCTGAGCATGTG+TGG	-	chr3_2:74251177-74251196	None:intergenic	55.0%
	GGCACTTCCTCGAAGTCATG+TGG	-	chr3_2:74251262-74251281	None:intergenic	55.0%
!	GAAGTGCCCTTTGAAGGGCT+CGG	+	chr3_2:74251274-74251293	Msa0424050:CDS	55.0%
	CACTTCTCCGAGCCCTTCAA+AGG	-	chr3_2:74251284-74251303	None:intergenic	55.0%
	TACACCAGGGGAAAGAAGGG+GGG	-	chr3_2:74251357-74251376	None:intergenic	55.0%
	CTACACCAGGGGAAAGAAGG+GGG	-	chr3_2:74251358-74251377	None:intergenic	55.0%
	TGAATGTCTGGAAGGGTCCC+GGG	-	chr3_2:74252143-74252162	None:intergenic	55.0%
	CAAACGGATGCCCTCACCTG+AGG	+	chr3_2:74250954-74250973	Msa0424050:CDS	60.0%
	GTCCAAGGGACCTCAGGTGA+GGG	-	chr3_2:74250967-74250986	None:intergenic	60.0%
	AGTCCAAGGGACCTCAGGTG+AGG	-	chr3_2:74250968-74250987	None:intergenic	60.0%
	CCTGAGCATGTGTGGAGAGC+AGG	-	chr3_2:74251169-74251188	None:intergenic	60.0%
	CCTGCTCTCCACACATGCTC+AGG	+	chr3_2:74251166-74251185	Msa0424050:CDS	60.0%
	CTCTCCACACATGCTCAGGG+AGG	+	chr3_2:74251170-74251189	Msa0424050:CDS	60.0%
	ACACCAGGGGAAAGAAGGGG+GGG	-	chr3_2:74251356-74251375	None:intergenic	60.0%
	TGCCCTCACCTGAGGTCCCT+TGG	+	chr3_2:74250962-74250981	Msa0424050:CDS	65.0%
	ACCAGGGGAAAGAAGGGGGG+GGG	-	chr3_2:74251354-74251373	None:intergenic	65.0%
	CACCAGGGGAAAGAAGGGGG+GGG	-	chr3_2:74251355-74251374	None:intergenic	65.0%
	AGGGGAAAGAAGGGGGGGGG+GGG	-	chr3_2:74251351-74251370	None:intergenic	70.0%
	CAGGGGAAAGAAGGGGGGGG+GGG	-	chr3_2:74251352-74251371	None:intergenic	70.0%
	CCAGGGGAAAGAAGGGGGGG+GGG	-	chr3_2:74251353-74251372	None:intergenic	70.0%
	AAAGAAGGGGGGGGGGGGGG+GGG	-	chr3_2:74251346-74251365	None:intergenic	75.0%
	GAAAGAAGGGGGGGGGGGGG+GGG	-	chr3_2:74251347-74251366	None:intergenic	75.0%
	GGAAAGAAGGGGGGGGGGGG+GGG	-	chr3_2:74251348-74251367	None:intergenic	75.0%
	GGGAAAGAAGGGGGGGGGGG+GGG	-	chr3_2:74251349-74251368	None:intergenic	75.0%
	GGGGAAAGAAGGGGGGGGGG+GGG	-	chr3_2:74251350-74251369	None:intergenic	75.0%
	CCCCCCCCCCTTCTTTCCCC+TGG	+	chr3_2:74251350-74251369	Msa0424050:intron	75.0%
!	AAGAAGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251345-74251364	None:intergenic	80.0%
!	AGAAGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251344-74251363	None:intergenic	85.0%
!	AAGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251342-74251361	None:intergenic	90.0%
!	GAAGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251343-74251362	None:intergenic	90.0%
!	GGGGGGGGGGGGGGGGGGAG+GGG	-	chr3_2:74251320-74251339	None:intergenic	95.0%
!	GGGGGGGGGGGGGGGGGGGA+GGG	-	chr3_2:74251321-74251340	None:intergenic	95.0%
!	AGGGGGGGGGGGGGGGGGGG+GGG	-	chr3_2:74251341-74251360	None:intergenic	95.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	74248260	74252507	74248260	ID=Msa0424050;Name=Msa0424050
chr3_2	mRNA	74248260	74252507	74248260	ID=Msa0424050-mRNA-1;Parent=Msa0424050;Name=Msa0424050-mRNA-1;_AED=0.08;_eAED=0.08;_QI=123\|0.8\|0.66\|0.83\|0.8\|0.66\|6\|0\|426
chr3_2	exon	74248260	74248344	74248260	ID=Msa0424050-mRNA-1:exon:18668;Parent=Msa0424050-mRNA-1
chr3_2	exon	74248945	74249233	74248945	ID=Msa0424050-mRNA-1:exon:18669;Parent=Msa0424050-mRNA-1
chr3_2	exon	74250184	74250532	74250184	ID=Msa0424050-mRNA-1:exon:18670;Parent=Msa0424050-mRNA-1
chr3_2	exon	74250631	74250772	74250631	ID=Msa0424050-mRNA-1:exon:18671;Parent=Msa0424050-mRNA-1
chr3_2	exon	74250933	74251300	74250933	ID=Msa0424050-mRNA-1:exon:18672;Parent=Msa0424050-mRNA-1
chr3_2	exon	74252337	74252507	74252337	ID=Msa0424050-mRNA-1:exon:18673;Parent=Msa0424050-mRNA-1
chr3_2	five_prime_UTR	74248260	74248344	74248260	ID=Msa0424050-mRNA-1:five_prime_utr;Parent=Msa0424050-mRNA-1
chr3_2	five_prime_UTR	74248945	74248982	74248945	ID=Msa0424050-mRNA-1:five_prime_utr;Parent=Msa0424050-mRNA-1
chr3_2	CDS	74248983	74249233	74248983	ID=Msa0424050-mRNA-1:cds;Parent=Msa0424050-mRNA-1
chr3_2	CDS	74250184	74250532	74250184	ID=Msa0424050-mRNA-1:cds;Parent=Msa0424050-mRNA-1
chr3_2	CDS	74250631	74250772	74250631	ID=Msa0424050-mRNA-1:cds;Parent=Msa0424050-mRNA-1
chr3_2	CDS	74250933	74251300	74250933	ID=Msa0424050-mRNA-1:cds;Parent=Msa0424050-mRNA-1
chr3_2	CDS	74252337	74252507	74252337	ID=Msa0424050-mRNA-1:cds;Parent=Msa0424050-mRNA-1

Gene Sequence

>Msa0424050

ATGGTTGGGAAGGATAATTTAAAGACTGAGGATTTGAATTTGTTTTTTGAGAATCTTATGATGGTTGCTGGTGGTGAGAAAGGAGGGAACATGAAGGTTGGTGTGATCACTGAATGGAAGGATATTCCTGTTGAGCTTTTGATGCAGATTCTGTCTCTTGTGGATGATCAGACGGTGATTCGAGCTTCTGGTGTTTGTCGTGGTTGGAGAGATTCTATTTACTTTGGTCTTGCTCGTTTATCACTTTCATGGTGTAACAAGAACATGAACAACTTGGTCCTATCTCTTGTTCCCAAGTTTGCAAAACTGCAAACTCTAATCCTTCGTCAAGATAAGCCACAACTGGACGACAATGTTGTTGGCACTATTGCAAATTTCTGTCATGACCTGCAGATTCTTGACCTCAGCAAAAGTTTCAAGCTCACTGATTGTTCACTGTATGCTATTGCTCATGGTTGCCGTGATCTGACAAAACTAAACATCAGTGGATGTTCAGCATTTAGTGACAATGCTCTGGCCTACCTTGCTGGTTTCTGTAGAAAACTGAAAGTTCTAAATCTATGTGGATGTGTTAGAGCAGCTTCTGATACTGCACTTCAGGCTATTGGACACTACTGCAATCAGTTGCAGTCTTTGAACCTTGGATGGTGTGACAAAGTTGGTGATGTTGGAGTGATGAGTCTAGCATATGGTTGTCCTGATCTAAGAACAGTTGATTTGTGTGGCTGTGTCTATATAACAGATGATAGTGTGATTGCTTTGGCAAACGGATGCCCTCACCTGAGGTCCCTTGGACTGTACTTCTGCAAGAACATAACAGACAATGCCATGTATTCATTGGCACAAAGCAAAGTGAAGAACAGGATGTGGGGATCTGTGAAAGGAGGAAATGATGAAGATGGACTAAGGACATTGAACATCAGCCAGTGCACTTCACTCACTCCTTCAGCTGTTCAGGCGGTTTGTGACTCATCTCCTGCTCTCCACACATGCTCAGGGAGGCATTCACTCATCATGAGTGGCTGTCTCAATTTGACCGAAGTCCATTGTGCTTGTGCTATTAATAAACCACATGACTTCGAGGAAGTGCCCTTTGAAGGGCTCGGAGAACCTTGTTTTGCAAAGCTTGTTCCAATCCACCCAATGAATCGTGTTCTCCATTTTTGCTCCTCACCAGAGGTTGCTAATCAGGCTTCCAATTTAGTCACGAATGTTCAATTGAATGAGAAAGCGGCTCATGAATGTAGTAGGTTAATGATAACTCCTTTACAAGCAAAGTGA

Protein sequence

>Msa0424050

MVGKDNLKTEDLNLFFENLMMVAGGEKGGNMKVGVITEWKDIPVELLMQILSLVDDQTVIRASGVCRGWRDSIYFGLARLSLSWCNKNMNNLVLSLVPKFAKLQTLILRQDKPQLDDNVVGTIANFCHDLQILDLSKSFKLTDCSLYAIAHGCRDLTKLNISGCSAFSDNALAYLAGFCRKLKVLNLCGCVRAASDTALQAIGHYCNQLQSLNLGWCDKVGDVGVMSLAYGCPDLRTVDLCGCVYITDDSVIALANGCPHLRSLGLYFCKNITDNAMYSLAQSKVKNRMWGSVKGGNDEDGLRTLNISQCTSLTPSAVQAVCDSSPALHTCSGRHSLIMSGCLNLTEVHCACAINKPHDFEEVPFEGLGEPCFAKLVPIHPMNRVLHFCSSPEVANQASNLVTNVQLNEKAAHECSRLMITPLQAK*