AlfalfaGEDB | Msa1440190

M. sativa cultivar zhongmu-4 / Msa1440190

KEGG: K00122
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0009507 GO:0009536 GO:0009579 GO:0010035 GO:0010038 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
PFAM: PF00389.33

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1440190	sp\|Q9SXP2\|FDH1_ORYSJ	78.042	378	48	3	1	353	1	368	0.0	582
Msa1440190	sp\|Q9SXP2\|FDH1_ORYSJ	80.737	353	43	2	358	685	24	376	0.0	575

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1440190	A0A2R6RYX7	74.533	750	106	5	21	685	19	768	0.0	1107

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004840	Msa1440190	0.821589	3.725137e-53	-8.615850e-47
Msa0015090	Msa1440190	0.820012	8.586357e-53	-8.615850e-47
Msa0016510	Msa1440190	0.860795	1.610208e-63	-8.615850e-47
Msa0020390	Msa1440190	0.836011	1.201512e-56	-8.615850e-47
Msa0038870	Msa1440190	0.808515	2.982537e-50	-8.615850e-47
Msa0047460	Msa1440190	0.970182	3.134765e-131	-8.615850e-47
Msa0049750	Msa1440190	0.825743	3.969202e-54	-8.615850e-47
Msa0065950	Msa1440190	0.833520	5.087760e-56	-8.615850e-47
Msa0074750	Msa1440190	0.840230	9.852604e-58	-8.615850e-47
Msa0081650	Msa1440190	0.800361	1.494227e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1440190	MtrunA17_Chr6g0450811	92.593	378	3	2	1	353	1	378	0.0	704
Msa1440190	MtrunA17_Chr6g0450811	85.873	361	26	2	350	685	26	386	0.0	634
Msa1440190	MtrunA17_Chr6g0450821	86.158	354	5	3	357	685	3	337	0.0	592
Msa1440190	MtrunA17_Chr6g0450821	79.310	348	28	3	31	353	1	329	0.0	539
Msa1440190	MtrunA17_Chr3g0077771	28.235	255	148	5	116	335	112	366	6.35e-18	86.3
Msa1440190	MtrunA17_Chr4g0000511	27.642	246	149	7	122	342	152	393	1.11e-16	84.0
Msa1440190	MtrunA17_Chr4g0000511	26.531	245	153	5	446	666	152	393	1.85e-16	83.2
Msa1440190	MtrunA17_Chr1g0208141	29.268	246	145	7	122	342	119	360	1.00e-15	80.9
Msa1440190	MtrunA17_Chr1g0208141	28.163	245	149	6	446	666	119	360	2.37e-15	79.7
Msa1440190	MtrunA17_Chr8g0351111	26.482	253	158	6	111	339	65	313	1.89e-15	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1440190	AT5G14780.2	76.423	369	60	3	342	685	4	370	0.0	539
Msa1440190	AT5G14780.2	77.746	346	52	2	33	353	17	362	0.0	521
Msa1440190	AT5G14780.1	77.716	359	55	2	352	685	26	384	0.0	538
Msa1440190	AT5G14780.1	74.142	379	69	4	1	353	1	376	0.0	525
Msa1440190	AT5G14780.3	78.592	355	51	2	356	685	4	358	0.0	538
Msa1440190	AT5G14780.3	77.746	346	52	2	33	353	5	350	0.0	521
Msa1440190	AT1G72190.1	30.213	235	132	5	116	320	110	342	1.35e-18	88.6
Msa1440190	AT1G72190.1	27.208	283	167	8	392	644	69	342	3.69e-18	87.4
Msa1440190	AT1G17745.1	27.599	279	170	9	90	342	115	387	2.74e-17	86.3
Msa1440190	AT1G17745.1	27.626	257	158	7	435	666	134	387	4.02e-17	85.9

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Find 178 sgRNAs with CRISPR-Local

Find 484 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATGAGGAGTCATCGCA+TGG	0.646699	tig0029037:+25614	None:intergenic
AACTACCTGTGCATCAATGG+TGG	0.632641	tig0029037:+30027	None:intergenic
AATATATGTAAAATTCTTAT+TGG	0.179143	tig0029037:+33540	None:intergenic
AATCTTCTTCTTTCCACCAG+AGG	0.597365	tig0029037:+31421	None:intergenic
AATGTGATCAGAACCAATGC+CGG	0.563596	tig0029037:+30954	None:intergenic
AATTCTTTCCGCAGTGACAT+AGG	0.516858	tig0029037:+31011	None:intergenic
AATTCTTTCCTCGGTGACAT+AGG	0.513371	tig0029037:+26574	None:intergenic
AATTGGAGCTCAGTTTGAGG+AGG	0.645215	tig0029037:-30617	Msa1440190:CDS
ACAGAACTACATTGTGAAGG+AGG	0.610756	tig0029037:-29370	Msa1440190:CDS
ACAGCATTACATTGTGAAGC+AGG	0.492555	tig0029037:-24907	Msa1440190:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGATTGAGCTTACATGA+AGG	+	tig0029037:25363-25382	None:intergenic	30.0%
TAAGAACTCAAAAGAAGCTT+TGG	-	tig0029037:25507-25526	Msa1440190:intron	30.0%
TTCCACAAAATTTAAAAGGG+GGG	+	tig0029037:26241-26260	None:intergenic	30.0%
TTGTGGAATAAAATTGGTGT+AGG	-	tig0029037:26253-26272	Msa1440190:CDS	30.0%
TGTGGAATAAAATTGGTGTA+GGG	-	tig0029037:26254-26273	Msa1440190:CDS	30.0%
ATTTCATTGGGTGAAAAAAC+TGG	+	tig0029037:26607-26626	None:intergenic	30.0%
GAAATGTTAGATGAAACTGA+CGG	+	tig0029037:26659-26678	None:intergenic	30.0%
TCAACACATCCAACAAAATT+GGG	+	tig0029037:27200-27219	None:intergenic	30.0%
TTCAACACATCCAACAAAAT+TGG	+	tig0029037:27201-27220	None:intergenic	30.0%
ATATCACGATAGACTTAAGA+TGG	-	tig0029037:27894-27913	Msa1440190:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0029037	gene	24881	33694	24881	ID=Msa1440190;Name=Msa1440190
tig0029037	mRNA	24881	33694	24881	ID=Msa1440190-mRNA-1;Parent=Msa1440190;Name=Msa1440190-mRNA-1;_AED=0.15;_eAED=0.16;_QI=263\|0.35\|0.33\|1\|0.5\|0.46\|15\|0\|685
tig0029037	exon	24881	24994	24881	ID=Msa1440190-mRNA-1:exon:12161;Parent=Msa1440190-mRNA-1
tig0029037	exon	25591	25703	25591	ID=Msa1440190-mRNA-1:exon:12160;Parent=Msa1440190-mRNA-1
tig0029037	exon	25864	25988	25864	ID=Msa1440190-mRNA-1:exon:12159;Parent=Msa1440190-mRNA-1
tig0029037	exon	26158	26384	26158	ID=Msa1440190-mRNA-1:exon:12158;Parent=Msa1440190-mRNA-1
tig0029037	exon	26415	26653	26415	ID=Msa1440190-mRNA-1:exon:12157;Parent=Msa1440190-mRNA-1
tig0029037	exon	26741	26912	26741	ID=Msa1440190-mRNA-1:exon:12156;Parent=Msa1440190-mRNA-1
tig0029037	exon	29148	29155	29148	ID=Msa1440190-mRNA-1:exon:12155;Parent=Msa1440190-mRNA-1
tig0029037	exon	29370	29457	29370	ID=Msa1440190-mRNA-1:exon:12154;Parent=Msa1440190-mRNA-1
tig0029037	exon	30033	30145	30033	ID=Msa1440190-mRNA-1:exon:12153;Parent=Msa1440190-mRNA-1
tig0029037	exon	30237	30361	30237	ID=Msa1440190-mRNA-1:exon:12152;Parent=Msa1440190-mRNA-1
tig0029037	exon	30595	30821	30595	ID=Msa1440190-mRNA-1:exon:12151;Parent=Msa1440190-mRNA-1
tig0029037	exon	30852	31090	30852	ID=Msa1440190-mRNA-1:exon:12150;Parent=Msa1440190-mRNA-1
tig0029037	exon	31273	31444	31273	ID=Msa1440190-mRNA-1:exon:12149;Parent=Msa1440190-mRNA-1
tig0029037	exon	33209	33340	33209	ID=Msa1440190-mRNA-1:exon:12148;Parent=Msa1440190-mRNA-1
tig0029037	exon	33468	33694	33468	ID=Msa1440190-mRNA-1:exon:12147;Parent=Msa1440190-mRNA-1
tig0029037	five_prime_UTR	33468	33694	33468	ID=Msa1440190-mRNA-1:five_prime_utr;Parent=Msa1440190-mRNA-1
tig0029037	five_prime_UTR	33305	33340	33305	ID=Msa1440190-mRNA-1:five_prime_utr;Parent=Msa1440190-mRNA-1
tig0029037	CDS	33209	33304	33209	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	31273	31444	31273	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	30852	31090	30852	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	30595	30821	30595	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	30237	30361	30237	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	30033	30145	30033	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	29370	29457	29370	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	29148	29155	29148	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	26741	26912	26741	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	26415	26653	26415	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	26158	26384	26158	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	25864	25988	25864	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	25591	25703	25591	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1
tig0029037	CDS	24881	24994	24881	ID=Msa1440190-mRNA-1:cds;Parent=Msa1440190-mRNA-1

Gene Sequence

>Msa1440190

ATGGCTATGAAACGTGCTGCTTCAACCTTGATCACTGCTTCTTCAAAGATTTCATCACTTTCTTCACCTTCTTCCATCATCACCAGGGACCTTCATGCCTCTGGTGGAAAGAAGAAGATTGTAGGTGTGTTCTACAAAGGCGGTGAATATGCTTCCTTGAATCCCAATTTTGTTGGATGTGTTGAAGGAGCATTAGGTATACGCGAGTGGTTGGAATCACAAGGACATGAGTACATTGTCACTGATGACAAAGAAGGACTCAATTCTGAACTCGAGAAGCACATTCCTGATCTCAATGTCCTGATATCCACACCATTTCACCCTGCCTATGTCACTGCGGAAAGAATTAAGAAAGCTAAGAATTTAGAGCTGCTTCTAACCGCCGGCATTGGTTCTGATCACATTGATCTCAATGCTGCGGCTGCTGCTGGTTTAACTGTCGCAGAGGTCACAGGAAGCAACACTGTGTCGGTTGCTGAGGATGAGCTCATGAGAATTCTCATTCTTGCTATTACCGGAGAATGGAATGTTGCTGGCATTGCACATAGAGCCTATGATCTTGAAGGAAAGACAGTTGGAACGGTCGGTGCTGGACGAATTGGGAAACTTTTACTCCAAAGGCTGAAACCCTTTAACTGTAACCTTTTATATCACGATAGACTTAAGATGGCGCCTGAATTGGAGAAAGAAATTGGAGCTCAGTTTGAGGAGGATCTTGATGCAATGCTTCCGAAAGGATTGTTTGACAAAAATAGAATTGCTAAGTTGAAGAAAGGTGTCTTGATTGTCAACAATGCTCGTGGAGCACTTATGGATACACAAGCAATTGCTGATGCTTGTTCTAGTGGACACGTTGCAGGTTACAGTGGTGATGTTTGGTTCCCACAACCAGCTCCAAAAGATCATCCATGGCGCTACATGCCTAATCATGCTATGACTCCTCATATTTCTGGAACCACCATTGATGCACAGTTACGTTATGCTGCTGGAGTCAAAGATATGCTTGAGAGGCACTTTAAGGGTGAAGACTTTCCTGAACAGAACTACATTGTGAAGGAGGTAATTAGTGCATCTGGAGGAAAGAAGAAAATTGTAGGTGTGTTCTACAAAGGCAATGAGAATGCTGCATCCAACCCCAATTTTGTTGGATGTGTTGAAGGAGCATTAGGTATACGCGAGTGGCTTGAATCACAAGGACATGAATACATTGTCACAGATGACAAAGAAGGACCTGATTCTGAACTCGAGAAGCACATTCCCGATCTGCATGTCCTGATATCTACACCATTTCACCCTGCCTATGTCACCGAGGAAAGAATTAAGAAAGCGAAGAATTTACAGTTGCTTTTAACTGCTGGCATTGGTTCCGATCACATTGATCTCAACGCTGCGGCTGCTGCTGGTTTAACCGTCGCAGAGGTCACAGGAAGCAACGTAGTATCGGTTGCTGAGGATGAGCTTATGAGAATTCTCATTCTCGCTGTTGCCGGAGAATGGAATGTTGCTGGTATTGCACATAGAGCCTATGATCTTGAAGGAAAGACGGTTGGAACAGTTGGTGCTGGACGAATCGGGAAGCTTTTACTCCAAAGGCTGAAACCCTTTAACTGTAACCTTTTATATCATGATAGACTTAAGATGGAGCCTGAATTGGAGAAAGAAATTGGAGCTCAGTTTGAGGACGACCTTGATACAATGCTACCAAAAGGATTGTTTGACAAAAATAGAATCGCTAAGTTGAAGAAAGGCGTCTTGATTGTTAACAATGCTCGTGGGGCGATTATGGACACACAAGCAGTTGCTGATGCTTGTACTAGTGGACACATTGCAGGTTACAGTGGTGATGTTTGGTTCCCACAACCAGCTCCAAAAGATCATCCATGGCGCTACATGCCAAACCATGCGATGACTCCTCATATTTCTGGAACTACCATTGATGCACAGTTACGTTATGCTGCTGGAGTCAAAGATATGCTTGAGAGGCACTTTAAGGGTGAGGACTTTCCTGAACAGCATTACATTGTGAAGCAGGGCCAATTGGCAAGCCAATACCGATGA

Protein sequence

>Msa1440190

MAMKRAASTLITASSKISSLSSPSSIITRDLHASGGKKKIVGVFYKGGEYASLNPNFVGCVEGALGIREWLESQGHEYIVTDDKEGLNSELEKHIPDLNVLISTPFHPAYVTAERIKKAKNLELLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNTVSVAEDELMRILILAITGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKMAPELEKEIGAQFEEDLDAMLPKGLFDKNRIAKLKKGVLIVNNARGALMDTQAIADACSSGHVAGYSGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLERHFKGEDFPEQNYIVKEVISASGGKKKIVGVFYKGNENAASNPNFVGCVEGALGIREWLESQGHEYIVTDDKEGPDSELEKHIPDLHVLISTPFHPAYVTEERIKKAKNLQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILAVAGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKMEPELEKEIGAQFEDDLDTMLPKGLFDKNRIAKLKKGVLIVNNARGAIMDTQAVADACTSGHIAGYSGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLERHFKGEDFPEQHYIVKQGQLASQYR*