AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0485700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485700 MtrunA17_Chr3g0109731 98.625 582 7 1 1 581 1 582 0.0 1196
Msa0485700 MtrunA17_Chr8g0370701 50.450 555 259 4 1 553 43 583 0.0 599
Msa0485700 MtrunA17_Chr5g0433241 53.286 563 233 8 1 551 1 545 0.0 589
Msa0485700 MtrunA17_Chr5g0396311 50.271 553 261 3 1 553 1 539 0.0 587
Msa0485700 MtrunA17_Chr4g0068151 51.792 558 254 8 1 551 7 556 0.0 575
Msa0485700 MtrunA17_Chr2g0301671 51.075 558 258 7 1 551 7 556 0.0 571
Msa0485700 MtrunA17_Chr5g0433231 51.792 558 250 6 1 551 1 546 0.0 555
Msa0485700 MtrunA17_Chr5g0396331 47.653 554 268 5 1 551 1 535 0.0 554
Msa0485700 MtrunA17_Chr5g0433221 50.538 558 257 7 1 551 1 546 0.0 544
Msa0485700 MtrunA17_Chr6g0471311 49.468 564 256 7 1 551 1 548 0.0 531
Msa0485700 MtrunA17_Chr8g0370731 49.284 489 232 4 67 553 20 494 5.82e-176 508
Msa0485700 MtrunA17_Chr5g0406681 41.367 556 301 11 3 551 29 566 3.05e-140 419
Msa0485700 MtrunA17_Chr4g0028411 26.642 548 359 12 14 552 27 540 1.14e-46 172
Msa0485700 MtrunA17_Chr5g0395431 28.388 546 341 16 18 549 73 582 1.08e-44 167
Msa0485700 MtrunA17_Chr2g0327341 27.848 553 343 15 14 552 27 537 1.02e-43 163
Msa0485700 MtrunA17_Chr6g0471921 28.804 552 337 15 15 554 5 512 1.95e-41 157
Msa0485700 MtrunA17_Chr1g0157401 26.577 523 336 13 37 546 65 552 6.71e-41 156
Msa0485700 MtrunA17_Chr1g0180541 27.007 548 350 16 14 546 28 540 1.73e-40 154
Msa0485700 MtrunA17_Chr3g0102411 26.486 555 333 17 19 549 33 536 2.05e-40 154
Msa0485700 MtrunA17_Chr4g0039791 26.975 519 314 14 49 547 81 554 5.29e-39 150
Msa0485700 MtrunA17_Chr4g0000461 28.131 551 335 17 25 556 36 544 1.18e-38 149
Msa0485700 MtrunA17_Chr8g0353341 27.143 490 297 14 37 501 62 516 1.77e-38 149
Msa0485700 MtrunA17_Chr4g0072561 26.354 554 341 16 18 549 35 543 2.78e-37 145
Msa0485700 MtrunA17_Chr1g0180551 26.259 556 348 16 14 546 97 613 3.06e-36 143
Msa0485700 MtrunA17_Chr5g0409791 26.214 515 333 14 49 547 78 561 1.02e-35 141
Msa0485700 MtrunA17_Chr5g0409771 26.565 527 330 16 36 547 64 548 3.03e-35 139
Msa0485700 MtrunA17_Chr1g0197391 25.849 530 334 16 43 556 82 568 6.50e-34 136
Msa0485700 MtrunA17_Chr2g0334131 33.448 290 172 8 266 549 5 279 4.08e-32 125
Msa0485700 MtrunA17_Chr3g0116251 27.989 368 223 8 194 546 208 548 7.47e-32 129
Msa0485700 MtrunA17_Chr3g0116271 27.671 365 231 7 194 546 207 550 1.23e-31 129
Msa0485700 MtrunA17_Chr1g0186071 24.470 519 335 9 39 549 82 551 2.02e-31 128
Msa0485700 MtrunA17_Chr2g0292471 25.000 552 343 20 39 547 27 550 1.99e-29 122
Msa0485700 MtrunA17_Chr4g0004101 28.980 245 155 7 306 547 96 324 1.42e-22 99.0
Msa0485700 MtrunA17_Chr4g0004841 29.528 254 146 9 306 547 101 333 2.24e-21 95.9
Msa0485700 MtrunA17_Chr7g0252691 23.723 548 351 18 40 550 206 723 4.91e-20 94.4
Msa0485700 MtrunA17_Chr1g0194371 24.644 491 283 18 41 469 78 543 1.73e-19 92.4
Msa0485700 MtrunA17_Chr8g0388081 23.636 550 297 23 41 508 19 527 3.38e-18 88.2
Msa0485700 MtrunA17_Chr1g0157431 26.667 285 185 7 37 312 65 334 1.07e-14 77.0
Msa0485700 MtrunA17_Chr5g0396691 21.689 521 291 18 41 480 80 564 4.50e-14 75.5
Msa0485700 MtrunA17_Chr7g0270221 29.439 214 137 6 259 469 301 503 1.08e-13 73.9
Msa0485700 MtrunA17_Chr3g0124631 33.000 200 100 8 306 484 426 612 4.18e-13 72.4
Msa0485700 MtrunA17_Chr5g0396711 25.588 340 171 14 191 469 226 544 7.94e-13 71.2
Msa0485700 MtrunA17_Chr1g0162621 20.492 488 307 14 40 469 79 543 2.33e-12 69.7
Msa0485700 MtrunA17_Chr1g0160041 32.948 173 94 6 306 469 424 583 7.37e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485700 AT1G65880.1 61.754 570 201 5 1 560 1 563 0.0 715
Msa0485700 AT1G65890.1 60.900 578 213 5 1 570 1 573 0.0 714
Msa0485700 AT1G66120.1 59.036 581 223 4 1 575 1 572 0.0 704
Msa0485700 AT1G68270.1 53.509 570 217 7 1 560 1 532 0.0 583
Msa0485700 AT1G20560.1 50.265 567 264 4 1 563 3 555 0.0 579
Msa0485700 AT3G16910.1 52.330 558 252 8 1 551 9 559 0.0 565
Msa0485700 AT5G16340.1 48.925 558 262 6 1 551 1 542 0.0 558
Msa0485700 AT5G16370.1 48.387 558 265 5 1 551 1 542 0.0 553
Msa0485700 AT2G17650.1 49.464 560 263 4 1 550 48 597 0.0 552
Msa0485700 AT1G21530.2 50.000 550 260 7 9 553 9 548 0.0 551
Msa0485700 AT1G21530.1 50.000 550 258 8 9 553 9 546 0.0 544
Msa0485700 AT1G21540.1 49.278 554 259 8 9 553 9 549 0.0 538
Msa0485700 AT1G75960.1 48.474 557 266 5 1 551 1 542 0.0 532
Msa0485700 AT1G77240.1 47.283 552 268 8 9 553 9 544 0.0 526
Msa0485700 AT1G20560.2 50.102 491 227 4 77 563 1 477 1.14e-171 496
Msa0485700 AT1G76290.1 45.324 556 278 8 1 551 1 535 7.01e-155 455
Msa0485700 AT3G21240.1 29.063 523 327 13 37 549 61 549 1.87e-49 180
Msa0485700 AT1G20510.1 27.687 549 329 20 18 546 36 536 1.52e-44 166
Msa0485700 AT1G51680.1 29.197 548 337 16 19 549 43 556 2.01e-44 166
Msa0485700 AT4G05160.1 26.895 554 346 14 17 552 30 542 4.84e-44 165
Msa0485700 AT4G19010.1 26.829 533 335 14 31 547 62 555 5.76e-43 162
Msa0485700 AT1G20500.1 25.046 543 351 14 18 546 42 542 1.25e-42 161
Msa0485700 AT1G20480.1 27.481 524 328 16 37 546 72 557 2.05e-42 160
Msa0485700 AT3G21230.1 28.653 527 328 12 37 549 71 563 1.14e-41 159
Msa0485700 AT1G65060.1 27.920 548 339 19 18 549 52 559 4.79e-41 157
Msa0485700 AT5G63380.1 26.190 546 326 16 17 546 66 550 2.01e-40 155
Msa0485700 AT5G38120.1 26.972 545 337 17 18 546 43 542 2.14e-38 149
Msa0485700 AT1G51680.3 28.627 517 318 16 19 518 43 525 3.15e-38 148
Msa0485700 AT3G48990.1 28.023 521 310 13 40 543 31 503 3.56e-38 148
Msa0485700 AT3G21240.2 27.872 470 300 13 19 476 41 483 1.41e-37 145
Msa0485700 AT3G21240.3 27.872 470 300 13 19 476 41 483 1.41e-37 145
Msa0485700 AT1G20510.2 28.033 478 283 18 18 475 36 472 1.10e-36 143
Msa0485700 AT5G38120.3 26.886 517 317 17 18 518 43 514 1.13e-33 135
Msa0485700 AT1G30520.2 25.225 555 371 15 20 549 13 548 9.66e-33 132
Msa0485700 AT1G30520.1 25.225 555 371 15 20 549 13 548 9.66e-33 132
Msa0485700 AT1G62940.1 26.071 560 340 16 14 549 26 535 1.72e-32 132
Msa0485700 AT1G51680.2 28.632 475 295 15 19 476 43 490 2.22e-32 130
Msa0485700 AT3G21230.3 27.419 496 312 12 37 518 71 532 8.15e-32 130
Msa0485700 AT1G20510.3 27.778 450 264 18 18 447 36 444 2.42e-30 124
Msa0485700 AT5G38120.4 26.160 474 296 13 18 475 43 478 6.27e-29 120
Msa0485700 AT5G38120.2 26.160 474 296 13 18 475 43 478 6.27e-29 120
Msa0485700 AT3G21230.2 27.533 454 288 11 37 476 71 497 3.60e-27 115
Msa0485700 AT1G65060.2 27.578 446 274 17 18 447 52 464 4.38e-27 115
Msa0485700 AT3G16170.1 25.000 388 240 11 189 547 236 601 3.10e-24 107
Msa0485700 AT1G20490.2 25.728 412 263 15 37 436 61 441 9.52e-23 101
Msa0485700 AT1G20490.1 25.728 412 263 15 37 436 61 441 9.52e-23 101
Msa0485700 AT1G64400.1 25.097 514 285 22 28 472 65 547 1.13e-21 99.8
Msa0485700 AT1G30520.3 23.881 469 319 12 20 471 13 460 2.62e-21 97.8
Msa0485700 AT1G30520.4 23.881 469 319 12 20 471 13 460 2.62e-21 97.8
Msa0485700 AT4G23850.1 24.517 518 280 20 41 480 81 565 4.54e-20 94.7
Msa0485700 AT5G36880.6 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0485700 AT5G36880.3 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0485700 AT5G36880.5 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0485700 AT5G36880.1 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0485700 AT5G36880.2 23.623 563 369 17 25 550 177 715 2.36e-18 89.4
Msa0485700 AT4G14070.1 21.658 591 321 23 20 501 102 659 7.43e-13 72.0
Msa0485700 AT4G11030.1 30.808 198 109 10 281 472 372 547 8.53e-12 68.6
Msa0485700 AT1G77590.2 32.558 172 96 6 306 469 274 433 1.31e-11 67.8
Msa0485700 AT1G77590.1 32.558 172 96 6 306 469 420 579 1.76e-11 67.4
Msa0485700 AT2G04350.2 30.418 263 138 13 306 551 449 683 5.51e-11 65.9
Msa0485700 AT2G04350.1 30.418 263 138 13 306 551 449 683 5.51e-11 65.9

Find 169 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAATTTACCACACTTTA+TGG 0.126746 3_4:+3435754 Msa0485700:CDS
TCTAAAGATGTGATTATTTC+AGG 0.185792 3_4:+3435536 Msa0485700:CDS
TAAAATAATGTTGAAAATAA+TGG 0.193559 3_4:-3435057 None:intergenic
CAAGGACTTTCACCCCATTT+TGG 0.195490 3_4:-3435647 None:intergenic
TGTCACATTATAGAGATTTA+TGG 0.205886 3_4:-3435018 None:intergenic
TATACATAAAGATGGTTATT+TGG 0.207931 3_4:+3435499 Msa0485700:CDS
ATTTCTTCAGGAAGGTAATT+TGG 0.214710 3_4:-3434735 None:intergenic
TATAACGTGGCCTTCAATTT+TGG 0.215714 3_4:+3433697 Msa0485700:five_prime_UTR
ATCTTACCTGTTAAAGTCTT+TGG 0.233560 3_4:-3435809 None:intergenic
TTATGATTGACTTGATTATT+AGG 0.237452 3_4:-3435899 None:intergenic
GCTCTCATCAAGAAAGTTAA+AGG 0.239728 3_4:-3433877 None:intergenic
CATGAGGCAAACACAAAATA+TGG 0.251810 3_4:-3436246 None:intergenic
AAGCGACGGCGAAAGCTTTC+GGG 0.254708 3_4:+3435444 Msa0485700:CDS
AAATGACTCAAATTAAATTA+TGG 0.260323 3_4:-3436203 None:intergenic
TATAGAAAATTTCATGTGAT+TGG 0.280157 3_4:-3433722 None:intergenic
GATTTACCAAAGACTTTAAC+AGG 0.288395 3_4:+3435803 Msa0485700:CDS
CTTCATAGTCACTAAATTAA+TGG 0.289137 3_4:+3433658 Msa0485700:five_prime_UTR
TTGCAATGCCACATCCAAAA+TGG 0.293682 3_4:+3435633 Msa0485700:CDS
AATAAAACAGGTATCAGTTT+TGG 0.306205 3_4:+3434398 Msa0485700:intron
TCATAATAATGACCAGATTA+TGG 0.314754 3_4:+3435916 Msa0485700:CDS
TTTCATGTAACACATGCTTA+TGG 0.320342 3_4:+3435185 Msa0485700:CDS
GTATAGTTTGCTCGACATTT+TGG 0.327519 3_4:-3433844 None:intergenic
GAAAGTTAAAGGAGTGAGTA+TGG 0.331539 3_4:-3433866 None:intergenic
CATTCATCTTGAATTTCTTC+AGG 0.339619 3_4:-3434747 None:intergenic
GAAGCGACGGCGAAAGCTTT+CGG 0.344503 3_4:+3435443 Msa0485700:CDS
TTTAATCCTTGGTTGGGAAA+TGG 0.345052 3_4:+3434863 Msa0485700:CDS
TGTGGTTAAGAATGAGATTA+GGG 0.348530 3_4:-3433755 None:intergenic
TGGCTATAAACAACACCTTT+TGG 0.356699 3_4:-3434813 None:intergenic
TTCTTCCATGAGTAATCTTA+AGG 0.361126 3_4:-3434592 None:intergenic
GGTAATGTCCATAGATAAAC+TGG 0.364652 3_4:-3434894 None:intergenic
GTTAGTTGTTTCCACTTAGT+AGG 0.368641 3_4:+3436157 Msa0485700:three_prime_UTR
AAAATAATGTTGAAAATAAT+GGG 0.371892 3_4:-3435056 None:intergenic
CGCATTGAGACGGTGGTTGA+TGG 0.387285 3_4:-3434487 None:intergenic
TCATACATTGCTGGAATGTT+TGG 0.387598 3_4:-3434423 None:intergenic
ATAGAAAATTTCATGTGATT+GGG 0.396517 3_4:-3433721 None:intergenic
AAGTGAAACGGGTTCCTTCA+TGG 0.397922 3_4:-3433933 None:intergenic
TACCACAAAACACTTAGCTT+TGG 0.397967 3_4:-3435852 None:intergenic
TATGGTCTGACTGAGGCAAC+AGG 0.406278 3_4:+3435203 Msa0485700:CDS
TGGTAAATTCTTCCTACAAT+AGG 0.411253 3_4:-3435744 None:intergenic
GTAAGAGATGGGAAAACAAT+GGG 0.423096 3_4:+3435368 Msa0485700:CDS
GATGTGTAATTCAATGTAAT+TGG 0.423792 3_4:-3434774 None:intergenic
TTTGAGTTTAATCCTTGGTT+GGG 0.424831 3_4:+3434857 Msa0485700:CDS
TTAATCCTTGGTTGGGAAAT+GGG 0.425386 3_4:+3434864 Msa0485700:CDS
TTTGTGAGTGGCAGAAGAAA+TGG 0.427336 3_4:+3435237 Msa0485700:CDS
TTACCTATGTTCCATTGTAA+TGG 0.427373 3_4:+3434912 Msa0485700:CDS
GAGCTCGCCTAGCCGGATTC+CGG 0.428306 3_4:-3434682 None:intergenic
CTTCATGGATGACTGAGGTT+CGG 0.429624 3_4:-3433918 None:intergenic
TTGTGGTTAAGAATGAGATT+AGG 0.431591 3_4:-3433756 None:intergenic
AAATGCATCTCATACATTGC+TGG 0.433221 3_4:-3434432 None:intergenic
GTTATTTGGAAATTAAAGAT+AGG 0.433555 3_4:+3435513 Msa0485700:CDS
GACGGTGGTTGATGGTGTTT+AGG 0.435440 3_4:-3434479 None:intergenic
GACGAGATAGAGCCATAATC+TGG 0.444504 3_4:-3435928 None:intergenic
GTAGTGAACCAGTTTATCTA+TGG 0.450766 3_4:+3434886 Msa0485700:CDS
TGCAATGCCACATCCAAAAT+GGG 0.459922 3_4:+3435634 Msa0485700:CDS
GGGGATGGTTGGTTTAGAAC+TGG 0.460142 3_4:+3435464 Msa0485700:CDS
ACACTTTATGGTACCTAAGA+TGG 0.462947 3_4:+3435766 Msa0485700:CDS
GAGATTATGTTGAAGGGAAG+TGG 0.465461 3_4:+3435392 Msa0485700:CDS
AGTTTGTGACCAAGTGAAAC+GGG 0.471117 3_4:-3433944 None:intergenic
CTGAAGAAATTCAAGATGAA+TGG 0.471358 3_4:+3434748 Msa0485700:CDS
ATGGAGAGTGTGGTAAGAGA+TGG 0.472523 3_4:+3435356 Msa0485700:CDS
GAAACAACTAACAAGAAAGT+TGG 0.473458 3_4:-3436146 None:intergenic
TAAACACTCCCACCGGAATC+CGG 0.473541 3_4:+3434670 Msa0485700:CDS
CTATGTTCCATTGTAATGGT+TGG 0.477230 3_4:+3434916 Msa0485700:CDS
CTTTGAGTTTAATCCTTGGT+TGG 0.482972 3_4:+3434856 Msa0485700:CDS
CGAAAGCTTTCGGGGATGGT+TGG 0.487803 3_4:+3435453 Msa0485700:CDS
CGCCTCAGAGTGTCGAAGAA+TGG 0.487931 3_4:-3434523 None:intergenic
ACAGCAGCTTCTAAAACCTT+AGG 0.490308 3_4:-3435608 None:intergenic
AAGTTTGTGACCAAGTGAAA+CGG 0.494196 3_4:-3433945 None:intergenic
TACTATCAAAGCAACTTCCT+TGG 0.494935 3_4:+3433600 None:intergenic
ACGGCGAAAGCTTTCGGGGA+TGG 0.495228 3_4:+3435449 Msa0485700:CDS
TTCACACTTCAACAATTTGA+GGG 0.495504 3_4:+3436036 Msa0485700:three_prime_UTR
TGCCACTCACAAACAAGTGC+TGG 0.499001 3_4:-3435227 None:intergenic
TGGTTTAGAACTGGTGATGT+TGG 0.499794 3_4:+3435473 Msa0485700:CDS
TCTCCGGTGAATATACTAAC+CGG 0.501022 3_4:+3435113 Msa0485700:CDS
ATGAGATAATTACCTATTGT+AGG 0.504104 3_4:+3435732 Msa0485700:CDS
TGGAGAGTGTGGTAAGAGAT+GGG 0.505965 3_4:+3435357 Msa0485700:CDS
ATTCGTATCAAAAGCAAAAG+AGG 0.506420 3_4:+3434569 Msa0485700:CDS
AAGGGAAGTGGAATCATGAT+GGG 0.506638 3_4:+3435404 Msa0485700:CDS
GTTCACACTTCAACAATTTG+AGG 0.509906 3_4:+3436035 Msa0485700:three_prime_UTR
TGCAATATTGAGAGCACGGA+GGG 0.514867 3_4:-3433995 None:intergenic
CATCTTGAATTTCTTCAGGA+AGG 0.517226 3_4:-3434743 None:intergenic
AGGAAGCGAGGCGGCGACAC+CGG 0.518047 3_4:-3433972 None:intergenic
AGAGGCCTTAAGATTACTCA+TGG 0.518068 3_4:+3434587 Msa0485700:CDS
CGTATTCGAGCTCGCCTAGC+CGG 0.519450 3_4:-3434689 None:intergenic
CTTGAGAAAATCGAGCCACT+TGG 0.519790 3_4:+3435161 Msa0485700:CDS
ATGAAGGAACCCGTTTCACT+TGG 0.520277 3_4:+3433935 Msa0485700:CDS
ATCTGCTTCAGCTTGTTATG+TGG 0.520395 3_4:+3436086 Msa0485700:three_prime_UTR
AGTACTTTGAGTTTAATCCT+TGG 0.521867 3_4:+3434852 Msa0485700:CDS
CTCGCCTAGCCGGATTCCGG+TGG 0.524552 3_4:-3434679 None:intergenic
ATTACATTGAATTACACATC+AGG 0.525480 3_4:+3434777 Msa0485700:CDS
CGAGCTCGAATACGAGCAAA+TGG 0.527362 3_4:+3434698 Msa0485700:CDS
TGTTCTTTATAGTCATCCTA+AGG 0.533616 3_4:+3435592 Msa0485700:CDS
GTGATTGGGACCAAAATTGA+AGG 0.536205 3_4:-3433707 None:intergenic
AACCGGTGGAGCACCACCCC+CGG 0.538649 3_4:+3435130 Msa0485700:CDS
TGTGACACTAAGGAAGAATG+AGG 0.538924 3_4:+3435676 Msa0485700:CDS
GAAGGGAAGTGGAATCATGA+TGG 0.539328 3_4:+3435403 Msa0485700:CDS
GTTGGTGTTATACATAAAGA+TGG 0.545404 3_4:+3435491 Msa0485700:CDS
AAAGAGAGTCATAAAATCTC+CGG 0.548039 3_4:+3435097 Msa0485700:CDS
TCGAAGAATGGTGGCTATGT+TGG 0.554863 3_4:-3434511 None:intergenic
CAACCATTACAATGGAACAT+AGG 0.559310 3_4:-3434915 None:intergenic
CACTACCCATTTCCCAACCA+AGG 0.560736 3_4:-3434869 None:intergenic
CACGGAGGGAGGAAGCGAGG+CGG 0.564152 3_4:-3433981 None:intergenic
GGTACAACTTCAGAACCAAA+AGG 0.565976 3_4:+3434798 Msa0485700:CDS
TCTTTGCAATATTGAGAGCA+CGG 0.566149 3_4:-3433999 None:intergenic
CGATGCTGAAAGCGCGACAA+GGG 0.572970 3_4:+3435285 Msa0485700:CDS
TACGAGCAAATGGTTCATCA+TGG 0.574224 3_4:+3434708 Msa0485700:CDS
GTAATGGTTGGACATTCACT+TGG 0.575202 3_4:+3434928 Msa0485700:CDS
CTTGGTCACAAACTTATGAC+CGG 0.576665 3_4:+3433953 Msa0485700:CDS
CATCTATCACAATCACTAGT+GGG 0.580127 3_4:-3434644 None:intergenic
TGGTTAAGAATGAGATTAGG+GGG 0.581716 3_4:-3433753 None:intergenic
TCGATGCTGAAAGCGCGACA+AGG 0.582751 3_4:+3435284 Msa0485700:CDS
GATGGTGTTTAGGACAGCAC+CGG 0.583797 3_4:-3434469 None:intergenic
TAATGGTTGGACATTCACTT+GGG 0.583819 3_4:+3434929 Msa0485700:CDS
GTAACTACAACTAATTCCAA+TGG 0.584509 3_4:+3433813 Msa0485700:exon
TTTAGGACAGCACCGGCCAT+AGG 0.587665 3_4:-3434462 None:intergenic
GAGCACGGAGGGAGGAAGCG+AGG 0.588763 3_4:-3433984 None:intergenic
GTTGTAGTTACCTAAGTGAG+AGG 0.592569 3_4:-3433801 None:intergenic
TCTTGAAACAATGGAGAGTG+TGG 0.595422 3_4:+3435346 Msa0485700:CDS
GGCCAGCACTTGTTTGTGAG+TGG 0.595693 3_4:+3435225 Msa0485700:CDS
TCATCTATCACAATCACTAG+TGG 0.596057 3_4:-3434645 None:intergenic
TGTGAAGAATCTTGAAACAA+TGG 0.596126 3_4:+3435337 Msa0485700:CDS
AAATTATGGAAGATATAACT+AGG 0.597180 3_4:-3436189 None:intergenic
ACTCCCACCGGAATCCGGCT+AGG 0.597247 3_4:+3434675 Msa0485700:CDS
AGCGACGGCGAAAGCTTTCG+GGG 0.597981 3_4:+3435445 Msa0485700:CDS
CGAACCTCAGTCATCCATGA+AGG 0.598539 3_4:+3433919 Msa0485700:CDS
AAGAGATGGGAAAACAATGG+GGG 0.600740 3_4:+3435370 Msa0485700:CDS
TTTCTCAAGTAGGGACGCCG+GGG 0.601501 3_4:-3435147 None:intergenic
CTATGTTGGCCGCATTGAGA+CGG 0.602111 3_4:-3434497 None:intergenic
GGACTGCTCAACCTACTAAG+TGG 0.602551 3_4:-3436168 None:intergenic
CTCAGAGTGTCGAAGAATGG+TGG 0.608683 3_4:-3434520 None:intergenic
TTGTAGTTACCTAAGTGAGA+GGG 0.608900 3_4:-3433800 None:intergenic
ACGTTATATGTGAAGAGGGT+GGG 0.610172 3_4:-3433682 None:intergenic
TGAATGTCCAACCATTACAA+TGG 0.613092 3_4:-3434923 None:intergenic
CCACCGGTTAGTATATTCAC+CGG 0.615355 3_4:-3435116 None:intergenic
GGCCACGTTATATGTGAAGA+GGG 0.615450 3_4:-3433686 None:intergenic
CACGTTATATGTGAAGAGGG+TGG 0.618052 3_4:-3433683 None:intergenic
GGTAAGAGATGGGAAAACAA+TGG 0.619269 3_4:+3435367 Msa0485700:CDS
ACTTGGGGTGTAGCTGCAAG+AGG 0.619778 3_4:+3434945 Msa0485700:CDS
AGGCCACGTTATATGTGAAG+AGG 0.621771 3_4:-3433687 None:intergenic
TAAGAGATGGGAAAACAATG+GGG 0.626561 3_4:+3435369 Msa0485700:CDS
GATGCTGAAAGCGCGACAAG+GGG 0.626984 3_4:+3435286 Msa0485700:CDS
TCGCCTAGCCGGATTCCGGT+GGG 0.628633 3_4:-3434678 None:intergenic
GATGCAGTGAAACTTGTATG+TGG 0.628757 3_4:-3433636 None:intergenic
AATATTGAGAGCACGGAGGG+AGG 0.632194 3_4:-3433992 None:intergenic
GATGACATAAACACTCCCAC+CGG 0.634917 3_4:+3434663 Msa0485700:CDS
CTTAGTGTCACAAAAGCACA+AGG 0.636960 3_4:-3435665 None:intergenic
TTCTCAAGTAGGGACGCCGG+GGG 0.637975 3_4:-3435146 None:intergenic
TGTTGGCCGCATTGAGACGG+TGG 0.638475 3_4:-3434494 None:intergenic
CACCATTCTTCGACACTCTG+AGG 0.640216 3_4:+3434521 Msa0485700:CDS
AATGGTTGGACATTCACTTG+GGG 0.644019 3_4:+3434930 Msa0485700:CDS
CACCCTCTTCACATATAACG+TGG 0.645497 3_4:+3433684 Msa0485700:five_prime_UTR
AAAGATGTGATTATTTCAGG+AGG 0.649768 3_4:+3435539 Msa0485700:CDS
TACTTAAATAAGCACCTCTG+TGG 0.656289 3_4:-3434833 None:intergenic
ATGGTCTGACTGAGGCAACA+GGG 0.656587 3_4:+3435204 Msa0485700:CDS
ATCTTAGGTACCATAAAGTG+TGG 0.661614 3_4:-3435764 None:intergenic
CCGGTGAATATACTAACCGG+TGG 0.668788 3_4:+3435116 Msa0485700:CDS
GTGGAAGTTATAGTATACCA+AGG 0.670695 3_4:-3433617 None:intergenic
AGGGAAGTGGAATCATGATG+GGG 0.670807 3_4:+3435405 Msa0485700:CDS
GTGGTTAAGAATGAGATTAG+GGG 0.674481 3_4:-3433754 None:intergenic
GACACTAAGGAAGAATGAGG+AGG 0.677688 3_4:+3435679 Msa0485700:CDS
TGGGGTGTAGCTGCAAGAGG+AGG 0.680125 3_4:+3434948 Msa0485700:CDS
GGTTAAGAATGAGATTAGGG+GGG 0.681147 3_4:-3433752 None:intergenic
GGTTCCTTCATGGATGACTG+AGG 0.686748 3_4:-3433923 None:intergenic
TGTGTTACATGAAAACCAAG+TGG 0.709272 3_4:-3435176 None:intergenic
GGTGTTGTTTATAGCCACAG+AGG 0.712162 3_4:+3434819 Msa0485700:CDS
ACATGCTTATGGTCTGACTG+AGG 0.729298 3_4:+3435196 Msa0485700:CDS
TTGCAATATTGAGAGCACGG+AGG 0.733139 3_4:-3433996 None:intergenic
CATCAACCACCGTCTCAATG+CGG 0.733801 3_4:+3434488 Msa0485700:CDS
GCAATGCCACATCCAAAATG+GGG 0.748056 3_4:+3435635 Msa0485700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATGTTGAAAATAAT+GGG - chr3_4:3435059-3435078 None:intergenic 10.0%
!! TAAAATAATGTTGAAAATAA+TGG - chr3_4:3435060-3435079 None:intergenic 10.0%
!!! ATTATTTTCAACATTATTTT+AGG + chr3_4:3435059-3435078 Msa0485700:CDS 10.0%
!! GAGATTTATAAAAACTAAAT+TGG + chr3_4:3434366-3434385 Msa0485700:intron 15.0%
!! AAAAAGTTCTTTATATATTG+TGG - chr3_4:3436121-3436140 None:intergenic 15.0%
!! AAATGACTCAAATTAAATTA+TGG - chr3_4:3436206-3436225 None:intergenic 15.0%
!! ATAGAAAATTTCATGTGATT+GGG - chr3_4:3433724-3433743 None:intergenic 20.0%
!! TATAGAAAATTTCATGTGAT+TGG - chr3_4:3433725-3433744 None:intergenic 20.0%
!! TATTACAATATTTGATACTG+CGG - chr3_4:3434285-3434304 None:intergenic 20.0%
!!! TGGTTTTTGTTTAATAAAAC+AGG + chr3_4:3434386-3434405 Msa0485700:intron 20.0%
!! TATACATAAAGATGGTTATT+TGG + chr3_4:3435499-3435518 Msa0485700:CDS 20.0%
!! GTTATTTGGAAATTAAAGAT+AGG + chr3_4:3435513-3435532 Msa0485700:CDS 20.0%
!!! TTATGATTGACTTGATTATT+AGG - chr3_4:3435902-3435921 None:intergenic 20.0%
!! AAATTATGGAAGATATAACT+AGG - chr3_4:3436192-3436211 None:intergenic 20.0%
! CTTCATAGTCACTAAATTAA+TGG + chr3_4:3433658-3433677 Msa0485700:five_prime_UTR 25.0%
!! AAATGAGAAATTTTGTGTTG+TGG - chr3_4:3433776-3433795 None:intergenic 25.0%
! CAAAATGCAGTATCAAATAT+TGG - chr3_4:3434261-3434280 None:intergenic 25.0%
!! AATAAAACAGGTATCAGTTT+TGG + chr3_4:3434398-3434417 Msa0485700:intron 25.0%
! GATGTGTAATTCAATGTAAT+TGG - chr3_4:3434777-3434796 None:intergenic 25.0%
! ATTACATTGAATTACACATC+AGG + chr3_4:3434777-3434796 Msa0485700:CDS 25.0%
! TGTCACATTATAGAGATTTA+TGG - chr3_4:3435021-3435040 None:intergenic 25.0%
!!! TTTTAAAGATAAAGAAGCGA+CGG + chr3_4:3435430-3435449 Msa0485700:CDS 25.0%
! TCTAAAGATGTGATTATTTC+AGG + chr3_4:3435536-3435555 Msa0485700:CDS 25.0%
! ATGAGATAATTACCTATTGT+AGG + chr3_4:3435732-3435751 Msa0485700:CDS 25.0%
! TCATAATAATGACCAGATTA+TGG + chr3_4:3435916-3435935 Msa0485700:CDS 25.0%
TGTGGTTAAGAATGAGATTA+GGG - chr3_4:3433758-3433777 None:intergenic 30.0%
TTGTGGTTAAGAATGAGATT+AGG - chr3_4:3433759-3433778 None:intergenic 30.0%
GTAACTACAACTAATTCCAA+TGG + chr3_4:3433813-3433832 Msa0485700:exon 30.0%
TGATATCATTTCCTAGATTG+CGG - chr3_4:3434223-3434242 None:intergenic 30.0%
ATTCGTATCAAAAGCAAAAG+AGG + chr3_4:3434569-3434588 Msa0485700:CDS 30.0%
TTCTTCCATGAGTAATCTTA+AGG - chr3_4:3434595-3434614 None:intergenic 30.0%
ATTTCTTCAGGAAGGTAATT+TGG - chr3_4:3434738-3434757 None:intergenic 30.0%
CATTCATCTTGAATTTCTTC+AGG - chr3_4:3434750-3434769 None:intergenic 30.0%
CTGAAGAAATTCAAGATGAA+TGG + chr3_4:3434748-3434767 Msa0485700:CDS 30.0%
! AGTACTTTGAGTTTAATCCT+TGG + chr3_4:3434852-3434871 Msa0485700:CDS 30.0%
! TTTGAGTTTAATCCTTGGTT+GGG + chr3_4:3434857-3434876 Msa0485700:CDS 30.0%
TTACCTATGTTCCATTGTAA+TGG + chr3_4:3434912-3434931 Msa0485700:CDS 30.0%
AAAGAGAGTCATAAAATCTC+CGG + chr3_4:3435097-3435116 Msa0485700:CDS 30.0%
TTTCATGTAACACATGCTTA+TGG + chr3_4:3435185-3435204 Msa0485700:CDS 30.0%
TGTGAAGAATCTTGAAACAA+TGG + chr3_4:3435337-3435356 Msa0485700:CDS 30.0%
!! GTTGGTGTTATACATAAAGA+TGG + chr3_4:3435491-3435510 Msa0485700:CDS 30.0%
AAAGATGTGATTATTTCAGG+AGG + chr3_4:3435539-3435558 Msa0485700:CDS 30.0%
TGTTCTTTATAGTCATCCTA+AGG + chr3_4:3435592-3435611 Msa0485700:CDS 30.0%
!! TGATTTTTGTTGTGTTACTG+AGG + chr3_4:3435709-3435728 Msa0485700:CDS 30.0%
TGGTAAATTCTTCCTACAAT+AGG - chr3_4:3435747-3435766 None:intergenic 30.0%
GAAGAATTTACCACACTTTA+TGG + chr3_4:3435754-3435773 Msa0485700:CDS 30.0%
!!! ACCTAAGATGGTTGTTTTTA+TGG + chr3_4:3435778-3435797 Msa0485700:CDS 30.0%
TCCATAAAAACAACCATCTT+AGG - chr3_4:3435782-3435801 None:intergenic 30.0%
!!! TAAGATGGTTGTTTTTATGG+AGG + chr3_4:3435781-3435800 Msa0485700:CDS 30.0%
GATTTACCAAAGACTTTAAC+AGG + chr3_4:3435803-3435822 Msa0485700:CDS 30.0%
ATCTTACCTGTTAAAGTCTT+TGG - chr3_4:3435812-3435831 None:intergenic 30.0%
TTCACACTTCAACAATTTGA+GGG + chr3_4:3436036-3436055 Msa0485700:three_prime_UTR 30.0%
GAAACAACTAACAAGAAAGT+TGG - chr3_4:3436149-3436168 None:intergenic 30.0%
GTGGAAGTTATAGTATACCA+AGG - chr3_4:3433620-3433639 None:intergenic 35.0%
! TATAACGTGGCCTTCAATTT+TGG + chr3_4:3433697-3433716 Msa0485700:five_prime_UTR 35.0%
TGGTTAAGAATGAGATTAGG+GGG - chr3_4:3433756-3433775 None:intergenic 35.0%
GTGGTTAAGAATGAGATTAG+GGG - chr3_4:3433757-3433776 None:intergenic 35.0%
!!! CATTTTTTTCCCTCTCACTT+AGG + chr3_4:3433791-3433810 Msa0485700:five_prime_UTR 35.0%
TTGTAGTTACCTAAGTGAGA+GGG - chr3_4:3433803-3433822 None:intergenic 35.0%
!! CATTTTGGTAGGTTATCCAT+TGG - chr3_4:3433832-3433851 None:intergenic 35.0%
!!! GTATAGTTTGCTCGACATTT+TGG - chr3_4:3433847-3433866 None:intergenic 35.0%
GAAAGTTAAAGGAGTGAGTA+TGG - chr3_4:3433869-3433888 None:intergenic 35.0%
GCTCTCATCAAGAAAGTTAA+AGG - chr3_4:3433880-3433899 None:intergenic 35.0%
AAGTTTGTGACCAAGTGAAA+CGG - chr3_4:3433948-3433967 None:intergenic 35.0%
TCTTTGCAATATTGAGAGCA+CGG - chr3_4:3434002-3434021 None:intergenic 35.0%
TCATACATTGCTGGAATGTT+TGG - chr3_4:3434426-3434445 None:intergenic 35.0%
AAATGCATCTCATACATTGC+TGG - chr3_4:3434435-3434454 None:intergenic 35.0%
CATCTATCACAATCACTAGT+GGG - chr3_4:3434647-3434666 None:intergenic 35.0%
TCATCTATCACAATCACTAG+TGG - chr3_4:3434648-3434667 None:intergenic 35.0%
CATCTTGAATTTCTTCAGGA+AGG - chr3_4:3434746-3434765 None:intergenic 35.0%
! TGGCTATAAACAACACCTTT+TGG - chr3_4:3434816-3434835 None:intergenic 35.0%
TACTTAAATAAGCACCTCTG+TGG - chr3_4:3434836-3434855 None:intergenic 35.0%
! CTTTGAGTTTAATCCTTGGT+TGG + chr3_4:3434856-3434875 Msa0485700:CDS 35.0%
TTTAATCCTTGGTTGGGAAA+TGG + chr3_4:3434863-3434882 Msa0485700:CDS 35.0%
TTAATCCTTGGTTGGGAAAT+GGG + chr3_4:3434864-3434883 Msa0485700:CDS 35.0%
GTAGTGAACCAGTTTATCTA+TGG + chr3_4:3434886-3434905 Msa0485700:CDS 35.0%
GGTAATGTCCATAGATAAAC+TGG - chr3_4:3434897-3434916 None:intergenic 35.0%
CAACCATTACAATGGAACAT+AGG - chr3_4:3434918-3434937 None:intergenic 35.0%
CTATGTTCCATTGTAATGGT+TGG + chr3_4:3434916-3434935 Msa0485700:CDS 35.0%
TGAATGTCCAACCATTACAA+TGG - chr3_4:3434926-3434945 None:intergenic 35.0%
TAATGGTTGGACATTCACTT+GGG + chr3_4:3434929-3434948 Msa0485700:CDS 35.0%
! TTTATGACTCTCTTTTCGCT+TGG - chr3_4:3435092-3435111 None:intergenic 35.0%
TGTGTTACATGAAAACCAAG+TGG - chr3_4:3435179-3435198 None:intergenic 35.0%
GTAAGAGATGGGAAAACAAT+GGG + chr3_4:3435368-3435387 Msa0485700:CDS 35.0%
TAAGAGATGGGAAAACAATG+GGG + chr3_4:3435369-3435388 Msa0485700:CDS 35.0%
ATCTTAGGTACCATAAAGTG+TGG - chr3_4:3435767-3435786 None:intergenic 35.0%
ACACTTTATGGTACCTAAGA+TGG + chr3_4:3435766-3435785 Msa0485700:CDS 35.0%
TACCACAAAACACTTAGCTT+TGG - chr3_4:3435855-3435874 None:intergenic 35.0%
GTTCACACTTCAACAATTTG+AGG + chr3_4:3436035-3436054 Msa0485700:three_prime_UTR 35.0%
! GTTAGTTGTTTCCACTTAGT+AGG + chr3_4:3436157-3436176 Msa0485700:three_prime_UTR 35.0%
GATGCAGTGAAACTTGTATG+TGG - chr3_4:3433639-3433658 None:intergenic 40.0%
ACGTTATATGTGAAGAGGGT+GGG - chr3_4:3433685-3433704 None:intergenic 40.0%
GTGATTGGGACCAAAATTGA+AGG - chr3_4:3433710-3433729 None:intergenic 40.0%
GGTTAAGAATGAGATTAGGG+GGG - chr3_4:3433755-3433774 None:intergenic 40.0%
GTTGTAGTTACCTAAGTGAG+AGG - chr3_4:3433804-3433823 None:intergenic 40.0%
!! AGTTTGCTCGACATTTTGGT+AGG - chr3_4:3433843-3433862 None:intergenic 40.0%
AGTTTGTGACCAAGTGAAAC+GGG - chr3_4:3433947-3433966 None:intergenic 40.0%
CTTGGTCACAAACTTATGAC+CGG + chr3_4:3433953-3433972 Msa0485700:CDS 40.0%
! GATGCATTTTGCAGTACCTA+TGG + chr3_4:3434446-3434465 Msa0485700:CDS 40.0%
AGAGGCCTTAAGATTACTCA+TGG + chr3_4:3434587-3434606 Msa0485700:CDS 40.0%
TACGAGCAAATGGTTCATCA+TGG + chr3_4:3434708-3434727 Msa0485700:CDS 40.0%
GGTACAACTTCAGAACCAAA+AGG + chr3_4:3434798-3434817 Msa0485700:CDS 40.0%
GTAATGGTTGGACATTCACT+TGG + chr3_4:3434928-3434947 Msa0485700:CDS 40.0%
AATGGTTGGACATTCACTTG+GGG + chr3_4:3434930-3434949 Msa0485700:CDS 40.0%
TCTCCGGTGAATATACTAAC+CGG + chr3_4:3435113-3435132 Msa0485700:CDS 40.0%
! TGGCTCGATTTTCTCAAGTA+GGG - chr3_4:3435159-3435178 None:intergenic 40.0%
TTTGTGAGTGGCAGAAGAAA+TGG + chr3_4:3435237-3435256 Msa0485700:CDS 40.0%
!!! AGCATCGATTGCTCTCTTTT+CGG - chr3_4:3435272-3435291 None:intergenic 40.0%
TCTTGAAACAATGGAGAGTG+TGG + chr3_4:3435346-3435365 Msa0485700:CDS 40.0%
GGTAAGAGATGGGAAAACAA+TGG + chr3_4:3435367-3435386 Msa0485700:CDS 40.0%
AAGAGATGGGAAAACAATGG+GGG + chr3_4:3435370-3435389 Msa0485700:CDS 40.0%
AATGGGGGAGATTATGTTGA+AGG + chr3_4:3435385-3435404 Msa0485700:CDS 40.0%
ATGGGGGAGATTATGTTGAA+GGG + chr3_4:3435386-3435405 Msa0485700:CDS 40.0%
GAGATTATGTTGAAGGGAAG+TGG + chr3_4:3435392-3435411 Msa0485700:CDS 40.0%
AAGGGAAGTGGAATCATGAT+GGG + chr3_4:3435404-3435423 Msa0485700:CDS 40.0%
! TGGTTTAGAACTGGTGATGT+TGG + chr3_4:3435473-3435492 Msa0485700:CDS 40.0%
!! ACAGCAGCTTCTAAAACCTT+AGG - chr3_4:3435611-3435630 None:intergenic 40.0%
TTGCAATGCCACATCCAAAA+TGG + chr3_4:3435633-3435652 Msa0485700:CDS 40.0%
TGCAATGCCACATCCAAAAT+GGG + chr3_4:3435634-3435653 Msa0485700:CDS 40.0%
! CTTAGTGTCACAAAAGCACA+AGG - chr3_4:3435668-3435687 None:intergenic 40.0%
! CTTGTGCTTTTGTGACACTA+AGG + chr3_4:3435666-3435685 Msa0485700:CDS 40.0%
TGTGACACTAAGGAAGAATG+AGG + chr3_4:3435676-3435695 Msa0485700:CDS 40.0%
! AGCCAAAGCTAAGTGTTTTG+TGG + chr3_4:3435850-3435869 Msa0485700:CDS 40.0%
!! ATCTGCTTCAGCTTGTTATG+TGG + chr3_4:3436086-3436105 Msa0485700:three_prime_UTR 40.0%
CACGTTATATGTGAAGAGGG+TGG - chr3_4:3433686-3433705 None:intergenic 45.0%
CACCCTCTTCACATATAACG+TGG + chr3_4:3433684-3433703 Msa0485700:five_prime_UTR 45.0%
GGCCACGTTATATGTGAAGA+GGG - chr3_4:3433689-3433708 None:intergenic 45.0%
AGGCCACGTTATATGTGAAG+AGG - chr3_4:3433690-3433709 None:intergenic 45.0%
CTTCATGGATGACTGAGGTT+CGG - chr3_4:3433921-3433940 None:intergenic 45.0%
! AAGTGAAACGGGTTCCTTCA+TGG - chr3_4:3433936-3433955 None:intergenic 45.0%
ATGAAGGAACCCGTTTCACT+TGG + chr3_4:3433935-3433954 Msa0485700:CDS 45.0%
! TGCAATATTGAGAGCACGGA+GGG - chr3_4:3433998-3434017 None:intergenic 45.0%
! TTGCAATATTGAGAGCACGG+AGG - chr3_4:3433999-3434018 None:intergenic 45.0%
! CATTTTGCAGTACCTATGGC+CGG + chr3_4:3434450-3434469 Msa0485700:CDS 45.0%
TCGAAGAATGGTGGCTATGT+TGG - chr3_4:3434514-3434533 None:intergenic 45.0%
GATGACATAAACACTCCCAC+CGG + chr3_4:3434663-3434682 Msa0485700:CDS 45.0%
!! GGTGTTGTTTATAGCCACAG+AGG + chr3_4:3434819-3434838 Msa0485700:CDS 45.0%
! CCACCGGTTAGTATATTCAC+CGG - chr3_4:3435119-3435138 None:intergenic 45.0%
! ATTTTCTCAAGTAGGGACGC+CGG - chr3_4:3435152-3435171 None:intergenic 45.0%
! GTGGCTCGATTTTCTCAAGT+AGG - chr3_4:3435160-3435179 None:intergenic 45.0%
CTTGAGAAAATCGAGCCACT+TGG + chr3_4:3435161-3435180 Msa0485700:CDS 45.0%
ACATGCTTATGGTCTGACTG+AGG + chr3_4:3435196-3435215 Msa0485700:CDS 45.0%
! ATGGAGAGTGTGGTAAGAGA+TGG + chr3_4:3435356-3435375 Msa0485700:CDS 45.0%
! TGGAGAGTGTGGTAAGAGAT+GGG + chr3_4:3435357-3435376 Msa0485700:CDS 45.0%
GAAGGGAAGTGGAATCATGA+TGG + chr3_4:3435403-3435422 Msa0485700:CDS 45.0%
AGGGAAGTGGAATCATGATG+GGG + chr3_4:3435405-3435424 Msa0485700:CDS 45.0%
GCAATGCCACATCCAAAATG+GGG + chr3_4:3435635-3435654 Msa0485700:CDS 45.0%
! CTTTCACCCCATTTTGGATG+TGG - chr3_4:3435644-3435663 None:intergenic 45.0%
! CAAGGACTTTCACCCCATTT+TGG - chr3_4:3435650-3435669 None:intergenic 45.0%
GACACTAAGGAAGAATGAGG+AGG + chr3_4:3435679-3435698 Msa0485700:CDS 45.0%
GACGAGATAGAGCCATAATC+TGG - chr3_4:3435931-3435950 None:intergenic 45.0%
CGAACCTCAGTCATCCATGA+AGG + chr3_4:3433919-3433938 Msa0485700:CDS 50.0%
GGTTCCTTCATGGATGACTG+AGG - chr3_4:3433926-3433945 None:intergenic 50.0%
! AATATTGAGAGCACGGAGGG+AGG - chr3_4:3433995-3434014 None:intergenic 50.0%
TAGATTGCGGTCGCTGATCT+AGG - chr3_4:3434210-3434229 None:intergenic 50.0%
TAGATCAGCGACCGCAATCT+AGG + chr3_4:3434209-3434228 Msa0485700:intron 50.0%
! GATGGTGTTTAGGACAGCAC+CGG - chr3_4:3434472-3434491 None:intergenic 50.0%
!! GACGGTGGTTGATGGTGTTT+AGG - chr3_4:3434482-3434501 None:intergenic 50.0%
CATCAACCACCGTCTCAATG+CGG + chr3_4:3434488-3434507 Msa0485700:CDS 50.0%
CTATGTTGGCCGCATTGAGA+CGG - chr3_4:3434500-3434519 None:intergenic 50.0%
!! CTCAGAGTGTCGAAGAATGG+TGG - chr3_4:3434523-3434542 None:intergenic 50.0%
CACCATTCTTCGACACTCTG+AGG + chr3_4:3434521-3434540 Msa0485700:CDS 50.0%
TAAACACTCCCACCGGAATC+CGG + chr3_4:3434670-3434689 Msa0485700:CDS 50.0%
CGAGCTCGAATACGAGCAAA+TGG + chr3_4:3434698-3434717 Msa0485700:CDS 50.0%
CACTACCCATTTCCCAACCA+AGG - chr3_4:3434872-3434891 None:intergenic 50.0%
CCGGTGAATATACTAACCGG+TGG + chr3_4:3435116-3435135 Msa0485700:CDS 50.0%
! TTTTCTCAAGTAGGGACGCC+GGG - chr3_4:3435151-3435170 None:intergenic 50.0%
TATGGTCTGACTGAGGCAAC+AGG + chr3_4:3435203-3435222 Msa0485700:CDS 50.0%
ATGGTCTGACTGAGGCAACA+GGG + chr3_4:3435204-3435223 Msa0485700:CDS 50.0%
TGCCACTCACAAACAAGTGC+TGG - chr3_4:3435230-3435249 None:intergenic 50.0%
!! GGGGATGGTTGGTTTAGAAC+TGG + chr3_4:3435464-3435483 Msa0485700:CDS 50.0%
GGACTGCTCAACCTACTAAG+TGG - chr3_4:3436171-3436190 None:intergenic 50.0%
!! TTTAGGACAGCACCGGCCAT+AGG - chr3_4:3434465-3434484 None:intergenic 55.0%
CGCATTGAGACGGTGGTTGA+TGG - chr3_4:3434490-3434509 None:intergenic 55.0%
!! CGCCTCAGAGTGTCGAAGAA+TGG - chr3_4:3434526-3434545 None:intergenic 55.0%
ACTTGGGGTGTAGCTGCAAG+AGG + chr3_4:3434945-3434964 Msa0485700:CDS 55.0%
TTTCTCAAGTAGGGACGCCG+GGG - chr3_4:3435150-3435169 None:intergenic 55.0%
GGCCAGCACTTGTTTGTGAG+TGG + chr3_4:3435225-3435244 Msa0485700:CDS 55.0%
! TCGATGCTGAAAGCGCGACA+AGG + chr3_4:3435284-3435303 Msa0485700:CDS 55.0%
! CGATGCTGAAAGCGCGACAA+GGG + chr3_4:3435285-3435304 Msa0485700:CDS 55.0%
! GATGCTGAAAGCGCGACAAG+GGG + chr3_4:3435286-3435305 Msa0485700:CDS 55.0%
GAAGCGACGGCGAAAGCTTT+CGG + chr3_4:3435443-3435462 Msa0485700:CDS 55.0%
AAGCGACGGCGAAAGCTTTC+GGG + chr3_4:3435444-3435463 Msa0485700:CDS 55.0%
CGAAAGCTTTCGGGGATGGT+TGG + chr3_4:3435453-3435472 Msa0485700:CDS 55.0%
TGTTGGCCGCATTGAGACGG+TGG - chr3_4:3434497-3434516 None:intergenic 60.0%
CGTATTCGAGCTCGCCTAGC+CGG - chr3_4:3434692-3434711 None:intergenic 60.0%
TGGGGTGTAGCTGCAAGAGG+AGG + chr3_4:3434948-3434967 Msa0485700:CDS 60.0%
TTCTCAAGTAGGGACGCCGG+GGG - chr3_4:3435149-3435168 None:intergenic 60.0%
AGCGACGGCGAAAGCTTTCG+GGG + chr3_4:3435445-3435464 Msa0485700:CDS 60.0%
ACGGCGAAAGCTTTCGGGGA+TGG + chr3_4:3435449-3435468 Msa0485700:CDS 60.0%
ACTCCCACCGGAATCCGGCT+AGG + chr3_4:3434675-3434694 Msa0485700:CDS 65.0%
TCGCCTAGCCGGATTCCGGT+GGG - chr3_4:3434681-3434700 None:intergenic 65.0%
GAGCTCGCCTAGCCGGATTC+CGG - chr3_4:3434685-3434704 None:intergenic 65.0%
AGGAAGCGAGGCGGCGACAC+CGG - chr3_4:3433975-3433994 None:intergenic 70.0%
CACGGAGGGAGGAAGCGAGG+CGG - chr3_4:3433984-3434003 None:intergenic 70.0%
GAGCACGGAGGGAGGAAGCG+AGG - chr3_4:3433987-3434006 None:intergenic 70.0%
CTCGCCTAGCCGGATTCCGG+TGG - chr3_4:3434682-3434701 None:intergenic 70.0%
! AACCGGTGGAGCACCACCCC+CGG + chr3_4:3435130-3435149 Msa0485700:CDS 70.0%
TCAAGTAGGGACGCCGGGGG+TGG - chr3_4:3435146-3435165 None:intergenic 70.0%
! CGCCGGGGGTGGTGCTCCAC+CGG - chr3_4:3435135-3435154 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 3433610 3436261 3433610 ID=Msa0485700;Name=Msa0485700
chr3_4 mRNA 3433610 3436261 3433610 ID=Msa0485700-mRNA-1;Parent=Msa0485700;Name=Msa0485700-mRNA-1;_AED=0.01;_eAED=0.01;_QI=222|1|1|1|1|1|2|306|581
chr3_4 exon 3433610 3434029 3433610 ID=Msa0485700-mRNA-1:exon:1231;Parent=Msa0485700-mRNA-1
chr3_4 exon 3434408 3436261 3434408 ID=Msa0485700-mRNA-1:exon:1232;Parent=Msa0485700-mRNA-1
chr3_4 five_prime_UTR 3433610 3433831 3433610 ID=Msa0485700-mRNA-1:five_prime_utr;Parent=Msa0485700-mRNA-1
chr3_4 CDS 3433832 3434029 3433832 ID=Msa0485700-mRNA-1:cds;Parent=Msa0485700-mRNA-1
chr3_4 CDS 3434408 3435955 3434408 ID=Msa0485700-mRNA-1:cds;Parent=Msa0485700-mRNA-1
chr3_4 three_prime_UTR 3435956 3436261 3435956 ID=Msa0485700-mRNA-1:three_prime_utr;Parent=Msa0485700-mRNA-1
Gene Sequence

>Msa0485700

ATGGATAACCTACCAAAATGTCGAGCAAACTATACCATACTCACTCCTTTAACTTTCTTGATGAGAGCTTCTGCATCTTATGCAAACCGAACCTCAGTCATCCATGAAGGAACCCGTTTCACTTGGTCACAAACTTATGACCGGTGTCGCCGCCTCGCTTCCTCCCTCCGTGCTCTCAATATTGCAAAGAATGATGTTGTATCAGTTTTGGCACCAAACATTCCAGCAATGTATGAGATGCATTTTGCAGTACCTATGGCCGGTGCTGTCCTAAACACCATCAACCACCGTCTCAATGCGGCCAACATAGCCACCATTCTTCGACACTCTGAGGCGAAAGTCTTCTTCGTAGACTACGAATTCGTATCAAAAGCAAAAGAGGCCTTAAGATTACTCATGGAAGAAAAAGATCAAAAAGAACAATACTCATCACTCCCACTAGTGATTGTGATAGATGACATAAACACTCCCACCGGAATCCGGCTAGGCGAGCTCGAATACGAGCAAATGGTTCATCATGGTAATCCAAATTACCTTCCTGAAGAAATTCAAGATGAATGGTCACCAATTACATTGAATTACACATCAGGTACAACTTCAGAACCAAAAGGTGTTGTTTATAGCCACAGAGGTGCTTATTTAAGTACTTTGAGTTTAATCCTTGGTTGGGAAATGGGTAGTGAACCAGTTTATCTATGGACATTACCTATGTTCCATTGTAATGGTTGGACATTCACTTGGGGTGTAGCTGCAAGAGGAGGTACTAATATTTGTATCAGAAACACTGCAGCATCTGATATATATAGAGCCATAAATCTCTATAATGTGACACACATGTGTTGCGCACCCATTATTTTCAACATTATTTTAGGCGCGAAACCAAGCGAAAAGAGAGTCATAAAATCTCCGGTGAATATACTAACCGGTGGAGCACCACCCCCGGCGTCCCTACTTGAGAAAATCGAGCCACTTGGTTTTCATGTAACACATGCTTATGGTCTGACTGAGGCAACAGGGCCAGCACTTGTTTGTGAGTGGCAGAAGAAATGGAATGTGTTGCCGAAAAGAGAGCAATCGATGCTGAAAGCGCGACAAGGGGTTAGTGTTTTGACACTTGCTGATGTTGATGTGAAGAATCTTGAAACAATGGAGAGTGTGGTAAGAGATGGGAAAACAATGGGGGAGATTATGTTGAAGGGAAGTGGAATCATGATGGGGTATTTTAAAGATAAAGAAGCGACGGCGAAAGCTTTCGGGGATGGTTGGTTTAGAACTGGTGATGTTGGTGTTATACATAAAGATGGTTATTTGGAAATTAAAGATAGGTCTAAAGATGTGATTATTTCAGGAGGTGAAAATATTAGTAGTGTTGAAGTTGAAAATGTTCTTTATAGTCATCCTAAGGTTTTAGAAGCTGCTGTTGTTGCAATGCCACATCCAAAATGGGGTGAAAGTCCTTGTGCTTTTGTGACACTAAGGAAGAATGAGGAGGAAAAAAGTGATTTTTGTTGTGTTACTGAGGATGAGATAATTACCTATTGTAGGAAGAATTTACCACACTTTATGGTACCTAAGATGGTTGTTTTTATGGAGGATTTACCAAAGACTTTAACAGGTAAGATTCAGAAATTTGAATTAAGAGCCAAAGCTAAGTGTTTTGTGGTAAATGATGAGAAGAATAAGAAGAAGCCTAATAATCAAGTCAATCATAATAATGACCAGATTATGGCTCTATCTCGTCTTTGA
Protein sequence

>Msa0485700

MDNLPKCRANYTILTPLTFLMRASASYANRTSVIHEGTRFTWSQTYDRCRRLASSLRALNIAKNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINHRLNAANIATILRHSEAKVFFVDYEFVSKAKEALRLLMEEKDQKEQYSSLPLVIVIDDINTPTGIRLGELEYEQMVHHGNPNYLPEEIQDEWSPITLNYTSGTTSEPKGVVYSHRGAYLSTLSLILGWEMGSEPVYLWTLPMFHCNGWTFTWGVAARGGTNICIRNTAASDIYRAINLYNVTHMCCAPIIFNIILGAKPSEKRVIKSPVNILTGGAPPPASLLEKIEPLGFHVTHAYGLTEATGPALVCEWQKKWNVLPKREQSMLKARQGVSVLTLADVDVKNLETMESVVRDGKTMGEIMLKGSGIMMGYFKDKEATAKAFGDGWFRTGDVGVIHKDGYLEIKDRSKDVIISGGENISSVEVENVLYSHPKVLEAAVVAMPHPKWGESPCAFVTLRKNEEEKSDFCCVTEDEIITYCRKNLPHFMVPKMVVFMEDLPKTLTGKIQKFELRAKAKCFVVNDEKNKKKPNNQVNHNNDQIMALSRL*