AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0689330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689330 MtrunA17_Chr3g0109731 98.625 582 7 1 1 581 1 582 0.0 1196
Msa0689330 MtrunA17_Chr8g0370701 50.450 555 259 4 1 553 43 583 0.0 599
Msa0689330 MtrunA17_Chr5g0433241 53.286 563 233 8 1 551 1 545 0.0 589
Msa0689330 MtrunA17_Chr5g0396311 50.271 553 261 3 1 553 1 539 0.0 587
Msa0689330 MtrunA17_Chr4g0068151 51.792 558 254 8 1 551 7 556 0.0 575
Msa0689330 MtrunA17_Chr2g0301671 51.075 558 258 7 1 551 7 556 0.0 571
Msa0689330 MtrunA17_Chr5g0433231 51.792 558 250 6 1 551 1 546 0.0 555
Msa0689330 MtrunA17_Chr5g0396331 47.653 554 268 5 1 551 1 535 0.0 554
Msa0689330 MtrunA17_Chr5g0433221 50.538 558 257 7 1 551 1 546 0.0 544
Msa0689330 MtrunA17_Chr6g0471311 49.468 564 256 7 1 551 1 548 0.0 531
Msa0689330 MtrunA17_Chr8g0370731 49.284 489 232 4 67 553 20 494 5.82e-176 508
Msa0689330 MtrunA17_Chr5g0406681 41.367 556 301 11 3 551 29 566 3.05e-140 419
Msa0689330 MtrunA17_Chr4g0028411 26.642 548 359 12 14 552 27 540 1.14e-46 172
Msa0689330 MtrunA17_Chr5g0395431 28.388 546 341 16 18 549 73 582 1.08e-44 167
Msa0689330 MtrunA17_Chr2g0327341 27.848 553 343 15 14 552 27 537 1.02e-43 163
Msa0689330 MtrunA17_Chr6g0471921 28.804 552 337 15 15 554 5 512 1.95e-41 157
Msa0689330 MtrunA17_Chr1g0157401 26.577 523 336 13 37 546 65 552 6.71e-41 156
Msa0689330 MtrunA17_Chr1g0180541 27.007 548 350 16 14 546 28 540 1.73e-40 154
Msa0689330 MtrunA17_Chr3g0102411 26.486 555 333 17 19 549 33 536 2.05e-40 154
Msa0689330 MtrunA17_Chr4g0039791 26.975 519 314 14 49 547 81 554 5.29e-39 150
Msa0689330 MtrunA17_Chr4g0000461 28.131 551 335 17 25 556 36 544 1.18e-38 149
Msa0689330 MtrunA17_Chr8g0353341 27.143 490 297 14 37 501 62 516 1.77e-38 149
Msa0689330 MtrunA17_Chr4g0072561 26.354 554 341 16 18 549 35 543 2.78e-37 145
Msa0689330 MtrunA17_Chr1g0180551 26.259 556 348 16 14 546 97 613 3.06e-36 143
Msa0689330 MtrunA17_Chr5g0409791 26.214 515 333 14 49 547 78 561 1.02e-35 141
Msa0689330 MtrunA17_Chr5g0409771 26.565 527 330 16 36 547 64 548 3.03e-35 139
Msa0689330 MtrunA17_Chr1g0197391 25.849 530 334 16 43 556 82 568 6.50e-34 136
Msa0689330 MtrunA17_Chr2g0334131 33.448 290 172 8 266 549 5 279 4.08e-32 125
Msa0689330 MtrunA17_Chr3g0116251 27.989 368 223 8 194 546 208 548 7.47e-32 129
Msa0689330 MtrunA17_Chr3g0116271 27.671 365 231 7 194 546 207 550 1.23e-31 129
Msa0689330 MtrunA17_Chr1g0186071 24.470 519 335 9 39 549 82 551 2.02e-31 128
Msa0689330 MtrunA17_Chr2g0292471 25.000 552 343 20 39 547 27 550 1.99e-29 122
Msa0689330 MtrunA17_Chr4g0004101 28.980 245 155 7 306 547 96 324 1.42e-22 99.0
Msa0689330 MtrunA17_Chr4g0004841 29.528 254 146 9 306 547 101 333 2.24e-21 95.9
Msa0689330 MtrunA17_Chr7g0252691 23.723 548 351 18 40 550 206 723 4.91e-20 94.4
Msa0689330 MtrunA17_Chr1g0194371 24.644 491 283 18 41 469 78 543 1.73e-19 92.4
Msa0689330 MtrunA17_Chr8g0388081 23.636 550 297 23 41 508 19 527 3.38e-18 88.2
Msa0689330 MtrunA17_Chr1g0157431 26.667 285 185 7 37 312 65 334 1.07e-14 77.0
Msa0689330 MtrunA17_Chr5g0396691 21.689 521 291 18 41 480 80 564 4.50e-14 75.5
Msa0689330 MtrunA17_Chr7g0270221 29.439 214 137 6 259 469 301 503 1.08e-13 73.9
Msa0689330 MtrunA17_Chr3g0124631 33.000 200 100 8 306 484 426 612 4.18e-13 72.4
Msa0689330 MtrunA17_Chr5g0396711 25.588 340 171 14 191 469 226 544 7.94e-13 71.2
Msa0689330 MtrunA17_Chr1g0162621 20.492 488 307 14 40 469 79 543 2.33e-12 69.7
Msa0689330 MtrunA17_Chr1g0160041 32.948 173 94 6 306 469 424 583 7.37e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689330 AT1G65880.1 61.754 570 201 5 1 560 1 563 0.0 715
Msa0689330 AT1G65890.1 60.900 578 213 5 1 570 1 573 0.0 714
Msa0689330 AT1G66120.1 59.036 581 223 4 1 575 1 572 0.0 704
Msa0689330 AT1G68270.1 53.509 570 217 7 1 560 1 532 0.0 583
Msa0689330 AT1G20560.1 50.265 567 264 4 1 563 3 555 0.0 579
Msa0689330 AT3G16910.1 52.330 558 252 8 1 551 9 559 0.0 565
Msa0689330 AT5G16340.1 48.925 558 262 6 1 551 1 542 0.0 558
Msa0689330 AT5G16370.1 48.387 558 265 5 1 551 1 542 0.0 553
Msa0689330 AT2G17650.1 49.464 560 263 4 1 550 48 597 0.0 552
Msa0689330 AT1G21530.2 50.000 550 260 7 9 553 9 548 0.0 551
Msa0689330 AT1G21530.1 50.000 550 258 8 9 553 9 546 0.0 544
Msa0689330 AT1G21540.1 49.278 554 259 8 9 553 9 549 0.0 538
Msa0689330 AT1G75960.1 48.474 557 266 5 1 551 1 542 0.0 532
Msa0689330 AT1G77240.1 47.283 552 268 8 9 553 9 544 0.0 526
Msa0689330 AT1G20560.2 50.102 491 227 4 77 563 1 477 1.14e-171 496
Msa0689330 AT1G76290.1 45.324 556 278 8 1 551 1 535 7.01e-155 455
Msa0689330 AT3G21240.1 29.063 523 327 13 37 549 61 549 1.87e-49 180
Msa0689330 AT1G20510.1 27.687 549 329 20 18 546 36 536 1.52e-44 166
Msa0689330 AT1G51680.1 29.197 548 337 16 19 549 43 556 2.01e-44 166
Msa0689330 AT4G05160.1 26.895 554 346 14 17 552 30 542 4.84e-44 165
Msa0689330 AT4G19010.1 26.829 533 335 14 31 547 62 555 5.76e-43 162
Msa0689330 AT1G20500.1 25.046 543 351 14 18 546 42 542 1.25e-42 161
Msa0689330 AT1G20480.1 27.481 524 328 16 37 546 72 557 2.05e-42 160
Msa0689330 AT3G21230.1 28.653 527 328 12 37 549 71 563 1.14e-41 159
Msa0689330 AT1G65060.1 27.920 548 339 19 18 549 52 559 4.79e-41 157
Msa0689330 AT5G63380.1 26.190 546 326 16 17 546 66 550 2.01e-40 155
Msa0689330 AT5G38120.1 26.972 545 337 17 18 546 43 542 2.14e-38 149
Msa0689330 AT1G51680.3 28.627 517 318 16 19 518 43 525 3.15e-38 148
Msa0689330 AT3G48990.1 28.023 521 310 13 40 543 31 503 3.56e-38 148
Msa0689330 AT3G21240.2 27.872 470 300 13 19 476 41 483 1.41e-37 145
Msa0689330 AT3G21240.3 27.872 470 300 13 19 476 41 483 1.41e-37 145
Msa0689330 AT1G20510.2 28.033 478 283 18 18 475 36 472 1.10e-36 143
Msa0689330 AT5G38120.3 26.886 517 317 17 18 518 43 514 1.13e-33 135
Msa0689330 AT1G30520.2 25.225 555 371 15 20 549 13 548 9.66e-33 132
Msa0689330 AT1G30520.1 25.225 555 371 15 20 549 13 548 9.66e-33 132
Msa0689330 AT1G62940.1 26.071 560 340 16 14 549 26 535 1.72e-32 132
Msa0689330 AT1G51680.2 28.632 475 295 15 19 476 43 490 2.22e-32 130
Msa0689330 AT3G21230.3 27.419 496 312 12 37 518 71 532 8.15e-32 130
Msa0689330 AT1G20510.3 27.778 450 264 18 18 447 36 444 2.42e-30 124
Msa0689330 AT5G38120.4 26.160 474 296 13 18 475 43 478 6.27e-29 120
Msa0689330 AT5G38120.2 26.160 474 296 13 18 475 43 478 6.27e-29 120
Msa0689330 AT3G21230.2 27.533 454 288 11 37 476 71 497 3.60e-27 115
Msa0689330 AT1G65060.2 27.578 446 274 17 18 447 52 464 4.38e-27 115
Msa0689330 AT3G16170.1 25.000 388 240 11 189 547 236 601 3.10e-24 107
Msa0689330 AT1G20490.2 25.728 412 263 15 37 436 61 441 9.52e-23 101
Msa0689330 AT1G20490.1 25.728 412 263 15 37 436 61 441 9.52e-23 101
Msa0689330 AT1G64400.1 25.097 514 285 22 28 472 65 547 1.13e-21 99.8
Msa0689330 AT1G30520.3 23.881 469 319 12 20 471 13 460 2.62e-21 97.8
Msa0689330 AT1G30520.4 23.881 469 319 12 20 471 13 460 2.62e-21 97.8
Msa0689330 AT4G23850.1 24.517 518 280 20 41 480 81 565 4.54e-20 94.7
Msa0689330 AT5G36880.6 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0689330 AT5G36880.3 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0689330 AT5G36880.5 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0689330 AT5G36880.1 23.623 563 369 17 25 550 127 665 1.89e-18 89.7
Msa0689330 AT5G36880.2 23.623 563 369 17 25 550 177 715 2.36e-18 89.4
Msa0689330 AT4G14070.1 21.658 591 321 23 20 501 102 659 7.43e-13 72.0
Msa0689330 AT4G11030.1 30.808 198 109 10 281 472 372 547 8.53e-12 68.6
Msa0689330 AT1G77590.2 32.558 172 96 6 306 469 274 433 1.31e-11 67.8
Msa0689330 AT1G77590.1 32.558 172 96 6 306 469 420 579 1.76e-11 67.4
Msa0689330 AT2G04350.2 30.418 263 138 13 306 551 449 683 5.51e-11 65.9
Msa0689330 AT2G04350.1 30.418 263 138 13 306 551 449 683 5.51e-11 65.9

Find 169 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAATTTACCACACTTTA+TGG 0.126746 4_4:-56363996 Msa0689330:CDS
TCTAAAGATGTGATTATTTC+AGG 0.185792 4_4:-56364214 Msa0689330:CDS
TAAAATAATGTTGAAAATAA+TGG 0.193559 4_4:+56364693 None:intergenic
CAAGGACTTTCACCCCATTT+TGG 0.195490 4_4:+56364103 None:intergenic
TGTCACATTATAGAGATTTA+TGG 0.205886 4_4:+56364732 None:intergenic
TATACATAAAGATGGTTATT+TGG 0.207931 4_4:-56364251 Msa0689330:CDS
ATTTCTTCAGGAAGGTAATT+TGG 0.214710 4_4:+56365015 None:intergenic
TATAACGTGGCCTTCAATTT+TGG 0.215714 4_4:-56366053 Msa0689330:five_prime_UTR
ATCTTACCTGTTAAAGTCTT+TGG 0.233560 4_4:+56363941 None:intergenic
TTATGATTGACTTGATTATT+AGG 0.237452 4_4:+56363851 None:intergenic
GCTCTCATCAAGAAAGTTAA+AGG 0.239728 4_4:+56365873 None:intergenic
CATGAGGCAAACACAAAATA+TGG 0.251810 4_4:+56363506 None:intergenic
AAGCGACGGCGAAAGCTTTC+GGG 0.254708 4_4:-56364306 Msa0689330:CDS
AAATGACTCAAATTAAATTA+TGG 0.260323 4_4:+56363549 None:intergenic
TATAGAAAATTTCATGTGAT+TGG 0.280157 4_4:+56366028 None:intergenic
GATTTACCAAAGACTTTAAC+AGG 0.288395 4_4:-56363947 Msa0689330:CDS
CTTCATAGTCACTAAATTAA+TGG 0.289137 4_4:-56366092 Msa0689330:five_prime_UTR
TTGCAATGCCACATCCAAAA+TGG 0.293682 4_4:-56364117 Msa0689330:CDS
AATAAAACAGGTATCAGTTT+TGG 0.306205 4_4:-56365352 Msa0689330:intron
TCATAATAATGACCAGATTA+TGG 0.314754 4_4:-56363834 Msa0689330:CDS
TTTCATGTAACACATGCTTA+TGG 0.320342 4_4:-56364565 Msa0689330:CDS
GTATAGTTTGCTCGACATTT+TGG 0.327519 4_4:+56365906 None:intergenic
GAAAGTTAAAGGAGTGAGTA+TGG 0.331539 4_4:+56365884 None:intergenic
CATTCATCTTGAATTTCTTC+AGG 0.339619 4_4:+56365003 None:intergenic
GAAGCGACGGCGAAAGCTTT+CGG 0.344503 4_4:-56364307 Msa0689330:CDS
TTTAATCCTTGGTTGGGAAA+TGG 0.345052 4_4:-56364887 Msa0689330:CDS
TGTGGTTAAGAATGAGATTA+GGG 0.348530 4_4:+56365995 None:intergenic
TGGCTATAAACAACACCTTT+TGG 0.356699 4_4:+56364937 None:intergenic
TTCTTCCATGAGTAATCTTA+AGG 0.361126 4_4:+56365158 None:intergenic
GGTAATGTCCATAGATAAAC+TGG 0.364652 4_4:+56364856 None:intergenic
GTTAGTTGTTTCCACTTAGT+AGG 0.368641 4_4:-56363595 Msa0689330:three_prime_UTR
AAAATAATGTTGAAAATAAT+GGG 0.371892 4_4:+56364694 None:intergenic
CGCATTGAGACGGTGGTTGA+TGG 0.387285 4_4:+56365263 None:intergenic
TCATACATTGCTGGAATGTT+TGG 0.387598 4_4:+56365327 None:intergenic
ATAGAAAATTTCATGTGATT+GGG 0.396517 4_4:+56366029 None:intergenic
AAGTGAAACGGGTTCCTTCA+TGG 0.397922 4_4:+56365817 None:intergenic
TACCACAAAACACTTAGCTT+TGG 0.397967 4_4:+56363898 None:intergenic
TATGGTCTGACTGAGGCAAC+AGG 0.406278 4_4:-56364547 Msa0689330:CDS
TGGTAAATTCTTCCTACAAT+AGG 0.411253 4_4:+56364006 None:intergenic
GTAAGAGATGGGAAAACAAT+GGG 0.423096 4_4:-56364382 Msa0689330:CDS
GATGTGTAATTCAATGTAAT+TGG 0.423792 4_4:+56364976 None:intergenic
TTTGAGTTTAATCCTTGGTT+GGG 0.424831 4_4:-56364893 Msa0689330:CDS
TTAATCCTTGGTTGGGAAAT+GGG 0.425386 4_4:-56364886 Msa0689330:CDS
TTTGTGAGTGGCAGAAGAAA+TGG 0.427336 4_4:-56364513 Msa0689330:CDS
TTACCTATGTTCCATTGTAA+TGG 0.427373 4_4:-56364838 Msa0689330:CDS
GAGCTCGCCTAGCCGGATTC+CGG 0.428306 4_4:+56365068 None:intergenic
CTTCATGGATGACTGAGGTT+CGG 0.429624 4_4:+56365832 None:intergenic
TTGTGGTTAAGAATGAGATT+AGG 0.431591 4_4:+56365994 None:intergenic
AAATGCATCTCATACATTGC+TGG 0.433221 4_4:+56365318 None:intergenic
GTTATTTGGAAATTAAAGAT+AGG 0.433555 4_4:-56364237 Msa0689330:CDS
GACGGTGGTTGATGGTGTTT+AGG 0.435440 4_4:+56365271 None:intergenic
GACGAGATAGAGCCATAATC+TGG 0.444504 4_4:+56363822 None:intergenic
GTAGTGAACCAGTTTATCTA+TGG 0.450766 4_4:-56364864 Msa0689330:CDS
TGCAATGCCACATCCAAAAT+GGG 0.459922 4_4:-56364116 Msa0689330:CDS
GGGGATGGTTGGTTTAGAAC+TGG 0.460142 4_4:-56364286 Msa0689330:CDS
ACACTTTATGGTACCTAAGA+TGG 0.462947 4_4:-56363984 Msa0689330:CDS
GAGATTATGTTGAAGGGAAG+TGG 0.465461 4_4:-56364358 Msa0689330:CDS
AGTTTGTGACCAAGTGAAAC+GGG 0.471117 4_4:+56365806 None:intergenic
CTGAAGAAATTCAAGATGAA+TGG 0.471358 4_4:-56365002 Msa0689330:CDS
ATGGAGAGTGTGGTAAGAGA+TGG 0.472523 4_4:-56364394 Msa0689330:CDS
GAAACAACTAACAAGAAAGT+TGG 0.473458 4_4:+56363606 None:intergenic
TAAACACTCCCACCGGAATC+CGG 0.473541 4_4:-56365080 Msa0689330:CDS
CTATGTTCCATTGTAATGGT+TGG 0.477230 4_4:-56364834 Msa0689330:CDS
CTTTGAGTTTAATCCTTGGT+TGG 0.482972 4_4:-56364894 Msa0689330:CDS
CGAAAGCTTTCGGGGATGGT+TGG 0.487803 4_4:-56364297 Msa0689330:CDS
CGCCTCAGAGTGTCGAAGAA+TGG 0.487931 4_4:+56365227 None:intergenic
ACAGCAGCTTCTAAAACCTT+AGG 0.490308 4_4:+56364142 None:intergenic
AAGTTTGTGACCAAGTGAAA+CGG 0.494196 4_4:+56365805 None:intergenic
TACTATCAAAGCAACTTCCT+TGG 0.494935 4_4:-56366150 None:intergenic
ACGGCGAAAGCTTTCGGGGA+TGG 0.495228 4_4:-56364301 Msa0689330:CDS
TTCACACTTCAACAATTTGA+GGG 0.495504 4_4:-56363714 Msa0689330:three_prime_UTR
TGCCACTCACAAACAAGTGC+TGG 0.499001 4_4:+56364523 None:intergenic
TGGTTTAGAACTGGTGATGT+TGG 0.499794 4_4:-56364277 Msa0689330:CDS
TCTCCGGTGAATATACTAAC+CGG 0.501022 4_4:-56364637 Msa0689330:CDS
ATGAGATAATTACCTATTGT+AGG 0.504104 4_4:-56364018 Msa0689330:CDS
TGGAGAGTGTGGTAAGAGAT+GGG 0.505965 4_4:-56364393 Msa0689330:CDS
ATTCGTATCAAAAGCAAAAG+AGG 0.506420 4_4:-56365181 Msa0689330:CDS
AAGGGAAGTGGAATCATGAT+GGG 0.506638 4_4:-56364346 Msa0689330:CDS
GTTCACACTTCAACAATTTG+AGG 0.509906 4_4:-56363715 Msa0689330:three_prime_UTR
TGCAATATTGAGAGCACGGA+GGG 0.514867 4_4:+56365755 None:intergenic
CATCTTGAATTTCTTCAGGA+AGG 0.517226 4_4:+56365007 None:intergenic
AGGAAGCGAGGCGGCGACAC+CGG 0.518047 4_4:+56365778 None:intergenic
AGAGGCCTTAAGATTACTCA+TGG 0.518068 4_4:-56365163 Msa0689330:CDS
CGTATTCGAGCTCGCCTAGC+CGG 0.519450 4_4:+56365061 None:intergenic
CTTGAGAAAATCGAGCCACT+TGG 0.519790 4_4:-56364589 Msa0689330:CDS
ATGAAGGAACCCGTTTCACT+TGG 0.520277 4_4:-56365815 Msa0689330:CDS
ATCTGCTTCAGCTTGTTATG+TGG 0.520395 4_4:-56363664 Msa0689330:three_prime_UTR
AGTACTTTGAGTTTAATCCT+TGG 0.521867 4_4:-56364898 Msa0689330:CDS
CTCGCCTAGCCGGATTCCGG+TGG 0.524552 4_4:+56365071 None:intergenic
ATTACATTGAATTACACATC+AGG 0.525480 4_4:-56364973 Msa0689330:CDS
CGAGCTCGAATACGAGCAAA+TGG 0.527362 4_4:-56365052 Msa0689330:CDS
TGTTCTTTATAGTCATCCTA+AGG 0.533616 4_4:-56364158 Msa0689330:CDS
GTGATTGGGACCAAAATTGA+AGG 0.536205 4_4:+56366043 None:intergenic
AACCGGTGGAGCACCACCCC+CGG 0.538649 4_4:-56364620 Msa0689330:CDS
TGTGACACTAAGGAAGAATG+AGG 0.538924 4_4:-56364074 Msa0689330:CDS
GAAGGGAAGTGGAATCATGA+TGG 0.539328 4_4:-56364347 Msa0689330:CDS
GTTGGTGTTATACATAAAGA+TGG 0.545404 4_4:-56364259 Msa0689330:CDS
AAAGAGAGTCATAAAATCTC+CGG 0.548039 4_4:-56364653 Msa0689330:CDS
TCGAAGAATGGTGGCTATGT+TGG 0.554863 4_4:+56365239 None:intergenic
CAACCATTACAATGGAACAT+AGG 0.559310 4_4:+56364835 None:intergenic
CACTACCCATTTCCCAACCA+AGG 0.560736 4_4:+56364881 None:intergenic
CACGGAGGGAGGAAGCGAGG+CGG 0.564152 4_4:+56365769 None:intergenic
GGTACAACTTCAGAACCAAA+AGG 0.565976 4_4:-56364952 Msa0689330:CDS
TCTTTGCAATATTGAGAGCA+CGG 0.566149 4_4:+56365751 None:intergenic
CGATGCTGAAAGCGCGACAA+GGG 0.572970 4_4:-56364465 Msa0689330:CDS
TACGAGCAAATGGTTCATCA+TGG 0.574224 4_4:-56365042 Msa0689330:CDS
GTAATGGTTGGACATTCACT+TGG 0.575202 4_4:-56364822 Msa0689330:CDS
CTTGGTCACAAACTTATGAC+CGG 0.576665 4_4:-56365797 Msa0689330:CDS
CATCTATCACAATCACTAGT+GGG 0.580127 4_4:+56365106 None:intergenic
TGGTTAAGAATGAGATTAGG+GGG 0.581716 4_4:+56365997 None:intergenic
TCGATGCTGAAAGCGCGACA+AGG 0.582751 4_4:-56364466 Msa0689330:CDS
GATGGTGTTTAGGACAGCAC+CGG 0.583797 4_4:+56365281 None:intergenic
TAATGGTTGGACATTCACTT+GGG 0.583819 4_4:-56364821 Msa0689330:CDS
GTAACTACAACTAATTCCAA+TGG 0.584509 4_4:-56365937 Msa0689330:exon
TTTAGGACAGCACCGGCCAT+AGG 0.587665 4_4:+56365288 None:intergenic
GAGCACGGAGGGAGGAAGCG+AGG 0.588763 4_4:+56365766 None:intergenic
GTTGTAGTTACCTAAGTGAG+AGG 0.592569 4_4:+56365949 None:intergenic
TCTTGAAACAATGGAGAGTG+TGG 0.595422 4_4:-56364404 Msa0689330:CDS
GGCCAGCACTTGTTTGTGAG+TGG 0.595693 4_4:-56364525 Msa0689330:CDS
TCATCTATCACAATCACTAG+TGG 0.596057 4_4:+56365105 None:intergenic
TGTGAAGAATCTTGAAACAA+TGG 0.596126 4_4:-56364413 Msa0689330:CDS
AAATTATGGAAGATATAACT+AGG 0.597180 4_4:+56363563 None:intergenic
ACTCCCACCGGAATCCGGCT+AGG 0.597247 4_4:-56365075 Msa0689330:CDS
AGCGACGGCGAAAGCTTTCG+GGG 0.597981 4_4:-56364305 Msa0689330:CDS
CGAACCTCAGTCATCCATGA+AGG 0.598539 4_4:-56365831 Msa0689330:CDS
AAGAGATGGGAAAACAATGG+GGG 0.600740 4_4:-56364380 Msa0689330:CDS
TTTCTCAAGTAGGGACGCCG+GGG 0.601501 4_4:+56364603 None:intergenic
CTATGTTGGCCGCATTGAGA+CGG 0.602111 4_4:+56365253 None:intergenic
GGACTGCTCAACCTACTAAG+TGG 0.602551 4_4:+56363584 None:intergenic
CTCAGAGTGTCGAAGAATGG+TGG 0.608683 4_4:+56365230 None:intergenic
TTGTAGTTACCTAAGTGAGA+GGG 0.608900 4_4:+56365950 None:intergenic
ACGTTATATGTGAAGAGGGT+GGG 0.610172 4_4:+56366068 None:intergenic
TGAATGTCCAACCATTACAA+TGG 0.613092 4_4:+56364827 None:intergenic
CCACCGGTTAGTATATTCAC+CGG 0.615355 4_4:+56364634 None:intergenic
GGCCACGTTATATGTGAAGA+GGG 0.615450 4_4:+56366064 None:intergenic
CACGTTATATGTGAAGAGGG+TGG 0.618052 4_4:+56366067 None:intergenic
GGTAAGAGATGGGAAAACAA+TGG 0.619269 4_4:-56364383 Msa0689330:CDS
ACTTGGGGTGTAGCTGCAAG+AGG 0.619778 4_4:-56364805 Msa0689330:CDS
AGGCCACGTTATATGTGAAG+AGG 0.621771 4_4:+56366063 None:intergenic
TAAGAGATGGGAAAACAATG+GGG 0.626561 4_4:-56364381 Msa0689330:CDS
GATGCTGAAAGCGCGACAAG+GGG 0.626984 4_4:-56364464 Msa0689330:CDS
TCGCCTAGCCGGATTCCGGT+GGG 0.628633 4_4:+56365072 None:intergenic
GATGCAGTGAAACTTGTATG+TGG 0.628757 4_4:+56366114 None:intergenic
AATATTGAGAGCACGGAGGG+AGG 0.632194 4_4:+56365758 None:intergenic
GATGACATAAACACTCCCAC+CGG 0.634917 4_4:-56365087 Msa0689330:CDS
CTTAGTGTCACAAAAGCACA+AGG 0.636960 4_4:+56364085 None:intergenic
TTCTCAAGTAGGGACGCCGG+GGG 0.637975 4_4:+56364604 None:intergenic
TGTTGGCCGCATTGAGACGG+TGG 0.638475 4_4:+56365256 None:intergenic
CACCATTCTTCGACACTCTG+AGG 0.640216 4_4:-56365229 Msa0689330:CDS
AATGGTTGGACATTCACTTG+GGG 0.644019 4_4:-56364820 Msa0689330:CDS
CACCCTCTTCACATATAACG+TGG 0.645497 4_4:-56366066 Msa0689330:five_prime_UTR
AAAGATGTGATTATTTCAGG+AGG 0.649768 4_4:-56364211 Msa0689330:CDS
TACTTAAATAAGCACCTCTG+TGG 0.656289 4_4:+56364917 None:intergenic
ATGGTCTGACTGAGGCAACA+GGG 0.656587 4_4:-56364546 Msa0689330:CDS
ATCTTAGGTACCATAAAGTG+TGG 0.661614 4_4:+56363986 None:intergenic
CCGGTGAATATACTAACCGG+TGG 0.668788 4_4:-56364634 Msa0689330:CDS
GTGGAAGTTATAGTATACCA+AGG 0.670695 4_4:+56366133 None:intergenic
AGGGAAGTGGAATCATGATG+GGG 0.670807 4_4:-56364345 Msa0689330:CDS
GTGGTTAAGAATGAGATTAG+GGG 0.674481 4_4:+56365996 None:intergenic
GACACTAAGGAAGAATGAGG+AGG 0.677688 4_4:-56364071 Msa0689330:CDS
TGGGGTGTAGCTGCAAGAGG+AGG 0.680125 4_4:-56364802 Msa0689330:CDS
GGTTAAGAATGAGATTAGGG+GGG 0.681147 4_4:+56365998 None:intergenic
GGTTCCTTCATGGATGACTG+AGG 0.686748 4_4:+56365827 None:intergenic
TGTGTTACATGAAAACCAAG+TGG 0.709272 4_4:+56364574 None:intergenic
GGTGTTGTTTATAGCCACAG+AGG 0.712162 4_4:-56364931 Msa0689330:CDS
ACATGCTTATGGTCTGACTG+AGG 0.729298 4_4:-56364554 Msa0689330:CDS
TTGCAATATTGAGAGCACGG+AGG 0.733139 4_4:+56365754 None:intergenic
CATCAACCACCGTCTCAATG+CGG 0.733801 4_4:-56365262 Msa0689330:CDS
GCAATGCCACATCCAAAATG+GGG 0.748056 4_4:-56364115 Msa0689330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATGTTGAAAATAAT+GGG + chr4_4:56364962-56364981 None:intergenic 10.0%
!! TAAAATAATGTTGAAAATAA+TGG + chr4_4:56364963-56364982 None:intergenic 10.0%
!!! ATTATTTTCAACATTATTTT+AGG - chr4_4:56364962-56364981 Msa0689330:CDS 10.0%
!! GAGATTTATAAAAACTAAAT+TGG - chr4_4:56364269-56364288 Msa0689330:CDS 15.0%
!! AAAAAGTTCTTTATATATTG+TGG + chr4_4:56366024-56366043 None:intergenic 15.0%
!! AAATGACTCAAATTAAATTA+TGG + chr4_4:56366107-56366126 None:intergenic 15.0%
!! ATAGAAAATTTCATGTGATT+GGG + chr4_4:56363627-56363646 None:intergenic 20.0%
!! TATAGAAAATTTCATGTGAT+TGG + chr4_4:56363628-56363647 None:intergenic 20.0%
!! TATTACAATATTTGATACTG+CGG + chr4_4:56364188-56364207 None:intergenic 20.0%
!!! TGGTTTTTGTTTAATAAAAC+AGG - chr4_4:56364289-56364308 Msa0689330:CDS 20.0%
!! TATACATAAAGATGGTTATT+TGG - chr4_4:56365402-56365421 Msa0689330:intron 20.0%
!! GTTATTTGGAAATTAAAGAT+AGG - chr4_4:56365416-56365435 Msa0689330:intron 20.0%
!!! TTATGATTGACTTGATTATT+AGG + chr4_4:56365805-56365824 None:intergenic 20.0%
!! AAATTATGGAAGATATAACT+AGG + chr4_4:56366093-56366112 None:intergenic 20.0%
! CTTCATAGTCACTAAATTAA+TGG - chr4_4:56363561-56363580 Msa0689330:three_prime_UTR 25.0%
!! AAATGAGAAATTTTGTGTTG+TGG + chr4_4:56363679-56363698 None:intergenic 25.0%
! CAAAATGCAGTATCAAATAT+TGG + chr4_4:56364164-56364183 None:intergenic 25.0%
!! AATAAAACAGGTATCAGTTT+TGG - chr4_4:56364301-56364320 Msa0689330:CDS 25.0%
! GATGTGTAATTCAATGTAAT+TGG + chr4_4:56364680-56364699 None:intergenic 25.0%
! ATTACATTGAATTACACATC+AGG - chr4_4:56364680-56364699 Msa0689330:CDS 25.0%
! TGTCACATTATAGAGATTTA+TGG + chr4_4:56364924-56364943 None:intergenic 25.0%
!!! TTTTAAAGATAAAGAAGCGA+CGG - chr4_4:56365333-56365352 Msa0689330:CDS 25.0%
! TCTAAAGATGTGATTATTTC+AGG - chr4_4:56365439-56365458 Msa0689330:intron 25.0%
! ATGAGATAATTACCTATTGT+AGG - chr4_4:56365635-56365654 Msa0689330:intron 25.0%
! TCATAATAATGACCAGATTA+TGG - chr4_4:56365819-56365838 Msa0689330:CDS 25.0%
TGTGGTTAAGAATGAGATTA+GGG + chr4_4:56363661-56363680 None:intergenic 30.0%
TTGTGGTTAAGAATGAGATT+AGG + chr4_4:56363662-56363681 None:intergenic 30.0%
GTAACTACAACTAATTCCAA+TGG - chr4_4:56363716-56363735 Msa0689330:three_prime_UTR 30.0%
TGATATCATTTCCTAGATTG+CGG + chr4_4:56364126-56364145 None:intergenic 30.0%
ATTCGTATCAAAAGCAAAAG+AGG - chr4_4:56364472-56364491 Msa0689330:CDS 30.0%
TTCTTCCATGAGTAATCTTA+AGG + chr4_4:56364498-56364517 None:intergenic 30.0%
ATTTCTTCAGGAAGGTAATT+TGG + chr4_4:56364641-56364660 None:intergenic 30.0%
CATTCATCTTGAATTTCTTC+AGG + chr4_4:56364653-56364672 None:intergenic 30.0%
CTGAAGAAATTCAAGATGAA+TGG - chr4_4:56364651-56364670 Msa0689330:CDS 30.0%
! AGTACTTTGAGTTTAATCCT+TGG - chr4_4:56364755-56364774 Msa0689330:CDS 30.0%
! TTTGAGTTTAATCCTTGGTT+GGG - chr4_4:56364760-56364779 Msa0689330:CDS 30.0%
TTACCTATGTTCCATTGTAA+TGG - chr4_4:56364815-56364834 Msa0689330:CDS 30.0%
AAAGAGAGTCATAAAATCTC+CGG - chr4_4:56365000-56365019 Msa0689330:CDS 30.0%
TTTCATGTAACACATGCTTA+TGG - chr4_4:56365088-56365107 Msa0689330:CDS 30.0%
TGTGAAGAATCTTGAAACAA+TGG - chr4_4:56365240-56365259 Msa0689330:CDS 30.0%
!! GTTGGTGTTATACATAAAGA+TGG - chr4_4:56365394-56365413 Msa0689330:intron 30.0%
AAAGATGTGATTATTTCAGG+AGG - chr4_4:56365442-56365461 Msa0689330:intron 30.0%
TGTTCTTTATAGTCATCCTA+AGG - chr4_4:56365495-56365514 Msa0689330:intron 30.0%
!! TGATTTTTGTTGTGTTACTG+AGG - chr4_4:56365612-56365631 Msa0689330:intron 30.0%
TGGTAAATTCTTCCTACAAT+AGG + chr4_4:56365650-56365669 None:intergenic 30.0%
GAAGAATTTACCACACTTTA+TGG - chr4_4:56365657-56365676 Msa0689330:intron 30.0%
!!! ACCTAAGATGGTTGTTTTTA+TGG - chr4_4:56365681-56365700 Msa0689330:intron 30.0%
TCCATAAAAACAACCATCTT+AGG + chr4_4:56365685-56365704 None:intergenic 30.0%
!!! TAAGATGGTTGTTTTTATGG+AGG - chr4_4:56365684-56365703 Msa0689330:intron 30.0%
GATTTACCAAAGACTTTAAC+AGG - chr4_4:56365706-56365725 Msa0689330:intron 30.0%
ATCTTACCTGTTAAAGTCTT+TGG + chr4_4:56365715-56365734 None:intergenic 30.0%
TTCACACTTCAACAATTTGA+GGG - chr4_4:56365939-56365958 Msa0689330:exon 30.0%
GAAACAACTAACAAGAAAGT+TGG + chr4_4:56366050-56366069 None:intergenic 30.0%
GTGGAAGTTATAGTATACCA+AGG + chr4_4:56363523-56363542 None:intergenic 35.0%
! TATAACGTGGCCTTCAATTT+TGG - chr4_4:56363600-56363619 Msa0689330:three_prime_UTR 35.0%
TGGTTAAGAATGAGATTAGG+GGG + chr4_4:56363659-56363678 None:intergenic 35.0%
GTGGTTAAGAATGAGATTAG+GGG + chr4_4:56363660-56363679 None:intergenic 35.0%
!!! CATTTTTTTCCCTCTCACTT+AGG - chr4_4:56363694-56363713 Msa0689330:three_prime_UTR 35.0%
TTGTAGTTACCTAAGTGAGA+GGG + chr4_4:56363706-56363725 None:intergenic 35.0%
!! CATTTTGGTAGGTTATCCAT+TGG + chr4_4:56363735-56363754 None:intergenic 35.0%
!!! GTATAGTTTGCTCGACATTT+TGG + chr4_4:56363750-56363769 None:intergenic 35.0%
GAAAGTTAAAGGAGTGAGTA+TGG + chr4_4:56363772-56363791 None:intergenic 35.0%
GCTCTCATCAAGAAAGTTAA+AGG + chr4_4:56363783-56363802 None:intergenic 35.0%
AAGTTTGTGACCAAGTGAAA+CGG + chr4_4:56363851-56363870 None:intergenic 35.0%
TCTTTGCAATATTGAGAGCA+CGG + chr4_4:56363905-56363924 None:intergenic 35.0%
TCATACATTGCTGGAATGTT+TGG + chr4_4:56364329-56364348 None:intergenic 35.0%
AAATGCATCTCATACATTGC+TGG + chr4_4:56364338-56364357 None:intergenic 35.0%
CATCTATCACAATCACTAGT+GGG + chr4_4:56364550-56364569 None:intergenic 35.0%
TCATCTATCACAATCACTAG+TGG + chr4_4:56364551-56364570 None:intergenic 35.0%
CATCTTGAATTTCTTCAGGA+AGG + chr4_4:56364649-56364668 None:intergenic 35.0%
! TGGCTATAAACAACACCTTT+TGG + chr4_4:56364719-56364738 None:intergenic 35.0%
TACTTAAATAAGCACCTCTG+TGG + chr4_4:56364739-56364758 None:intergenic 35.0%
! CTTTGAGTTTAATCCTTGGT+TGG - chr4_4:56364759-56364778 Msa0689330:CDS 35.0%
TTTAATCCTTGGTTGGGAAA+TGG - chr4_4:56364766-56364785 Msa0689330:CDS 35.0%
TTAATCCTTGGTTGGGAAAT+GGG - chr4_4:56364767-56364786 Msa0689330:CDS 35.0%
GTAGTGAACCAGTTTATCTA+TGG - chr4_4:56364789-56364808 Msa0689330:CDS 35.0%
GGTAATGTCCATAGATAAAC+TGG + chr4_4:56364800-56364819 None:intergenic 35.0%
CAACCATTACAATGGAACAT+AGG + chr4_4:56364821-56364840 None:intergenic 35.0%
CTATGTTCCATTGTAATGGT+TGG - chr4_4:56364819-56364838 Msa0689330:CDS 35.0%
TGAATGTCCAACCATTACAA+TGG + chr4_4:56364829-56364848 None:intergenic 35.0%
TAATGGTTGGACATTCACTT+GGG - chr4_4:56364832-56364851 Msa0689330:CDS 35.0%
! TTTATGACTCTCTTTTCGCT+TGG + chr4_4:56364995-56365014 None:intergenic 35.0%
TGTGTTACATGAAAACCAAG+TGG + chr4_4:56365082-56365101 None:intergenic 35.0%
GTAAGAGATGGGAAAACAAT+GGG - chr4_4:56365271-56365290 Msa0689330:CDS 35.0%
TAAGAGATGGGAAAACAATG+GGG - chr4_4:56365272-56365291 Msa0689330:CDS 35.0%
ATCTTAGGTACCATAAAGTG+TGG + chr4_4:56365670-56365689 None:intergenic 35.0%
ACACTTTATGGTACCTAAGA+TGG - chr4_4:56365669-56365688 Msa0689330:intron 35.0%
TACCACAAAACACTTAGCTT+TGG + chr4_4:56365758-56365777 None:intergenic 35.0%
GTTCACACTTCAACAATTTG+AGG - chr4_4:56365938-56365957 Msa0689330:exon 35.0%
! GTTAGTTGTTTCCACTTAGT+AGG - chr4_4:56366058-56366077 Msa0689330:five_prime_UTR 35.0%
GATGCAGTGAAACTTGTATG+TGG + chr4_4:56363542-56363561 None:intergenic 40.0%
ACGTTATATGTGAAGAGGGT+GGG + chr4_4:56363588-56363607 None:intergenic 40.0%
GTGATTGGGACCAAAATTGA+AGG + chr4_4:56363613-56363632 None:intergenic 40.0%
GGTTAAGAATGAGATTAGGG+GGG + chr4_4:56363658-56363677 None:intergenic 40.0%
GTTGTAGTTACCTAAGTGAG+AGG + chr4_4:56363707-56363726 None:intergenic 40.0%
!! AGTTTGCTCGACATTTTGGT+AGG + chr4_4:56363746-56363765 None:intergenic 40.0%
AGTTTGTGACCAAGTGAAAC+GGG + chr4_4:56363850-56363869 None:intergenic 40.0%
CTTGGTCACAAACTTATGAC+CGG - chr4_4:56363856-56363875 Msa0689330:CDS 40.0%
! GATGCATTTTGCAGTACCTA+TGG - chr4_4:56364349-56364368 Msa0689330:CDS 40.0%
AGAGGCCTTAAGATTACTCA+TGG - chr4_4:56364490-56364509 Msa0689330:CDS 40.0%
TACGAGCAAATGGTTCATCA+TGG - chr4_4:56364611-56364630 Msa0689330:CDS 40.0%
GGTACAACTTCAGAACCAAA+AGG - chr4_4:56364701-56364720 Msa0689330:CDS 40.0%
GTAATGGTTGGACATTCACT+TGG - chr4_4:56364831-56364850 Msa0689330:CDS 40.0%
AATGGTTGGACATTCACTTG+GGG - chr4_4:56364833-56364852 Msa0689330:CDS 40.0%
TCTCCGGTGAATATACTAAC+CGG - chr4_4:56365016-56365035 Msa0689330:CDS 40.0%
! TGGCTCGATTTTCTCAAGTA+GGG + chr4_4:56365062-56365081 None:intergenic 40.0%
TTTGTGAGTGGCAGAAGAAA+TGG - chr4_4:56365140-56365159 Msa0689330:CDS 40.0%
!!! AGCATCGATTGCTCTCTTTT+CGG + chr4_4:56365175-56365194 None:intergenic 40.0%
TCTTGAAACAATGGAGAGTG+TGG - chr4_4:56365249-56365268 Msa0689330:CDS 40.0%
GGTAAGAGATGGGAAAACAA+TGG - chr4_4:56365270-56365289 Msa0689330:CDS 40.0%
AAGAGATGGGAAAACAATGG+GGG - chr4_4:56365273-56365292 Msa0689330:CDS 40.0%
AATGGGGGAGATTATGTTGA+AGG - chr4_4:56365288-56365307 Msa0689330:CDS 40.0%
ATGGGGGAGATTATGTTGAA+GGG - chr4_4:56365289-56365308 Msa0689330:CDS 40.0%
GAGATTATGTTGAAGGGAAG+TGG - chr4_4:56365295-56365314 Msa0689330:CDS 40.0%
AAGGGAAGTGGAATCATGAT+GGG - chr4_4:56365307-56365326 Msa0689330:CDS 40.0%
! TGGTTTAGAACTGGTGATGT+TGG - chr4_4:56365376-56365395 Msa0689330:intron 40.0%
!! ACAGCAGCTTCTAAAACCTT+AGG + chr4_4:56365514-56365533 None:intergenic 40.0%
TTGCAATGCCACATCCAAAA+TGG - chr4_4:56365536-56365555 Msa0689330:intron 40.0%
TGCAATGCCACATCCAAAAT+GGG - chr4_4:56365537-56365556 Msa0689330:intron 40.0%
! CTTAGTGTCACAAAAGCACA+AGG + chr4_4:56365571-56365590 None:intergenic 40.0%
! CTTGTGCTTTTGTGACACTA+AGG - chr4_4:56365569-56365588 Msa0689330:intron 40.0%
TGTGACACTAAGGAAGAATG+AGG - chr4_4:56365579-56365598 Msa0689330:intron 40.0%
! AGCCAAAGCTAAGTGTTTTG+TGG - chr4_4:56365753-56365772 Msa0689330:CDS 40.0%
!! ATCTGCTTCAGCTTGTTATG+TGG - chr4_4:56365989-56366008 Msa0689330:five_prime_UTR 40.0%
CACGTTATATGTGAAGAGGG+TGG + chr4_4:56363589-56363608 None:intergenic 45.0%
CACCCTCTTCACATATAACG+TGG - chr4_4:56363587-56363606 Msa0689330:three_prime_UTR 45.0%
GGCCACGTTATATGTGAAGA+GGG + chr4_4:56363592-56363611 None:intergenic 45.0%
AGGCCACGTTATATGTGAAG+AGG + chr4_4:56363593-56363612 None:intergenic 45.0%
CTTCATGGATGACTGAGGTT+CGG + chr4_4:56363824-56363843 None:intergenic 45.0%
! AAGTGAAACGGGTTCCTTCA+TGG + chr4_4:56363839-56363858 None:intergenic 45.0%
ATGAAGGAACCCGTTTCACT+TGG - chr4_4:56363838-56363857 Msa0689330:CDS 45.0%
! TGCAATATTGAGAGCACGGA+GGG + chr4_4:56363901-56363920 None:intergenic 45.0%
! TTGCAATATTGAGAGCACGG+AGG + chr4_4:56363902-56363921 None:intergenic 45.0%
! CATTTTGCAGTACCTATGGC+CGG - chr4_4:56364353-56364372 Msa0689330:CDS 45.0%
TCGAAGAATGGTGGCTATGT+TGG + chr4_4:56364417-56364436 None:intergenic 45.0%
GATGACATAAACACTCCCAC+CGG - chr4_4:56364566-56364585 Msa0689330:CDS 45.0%
!! GGTGTTGTTTATAGCCACAG+AGG - chr4_4:56364722-56364741 Msa0689330:CDS 45.0%
! CCACCGGTTAGTATATTCAC+CGG + chr4_4:56365022-56365041 None:intergenic 45.0%
! ATTTTCTCAAGTAGGGACGC+CGG + chr4_4:56365055-56365074 None:intergenic 45.0%
! GTGGCTCGATTTTCTCAAGT+AGG + chr4_4:56365063-56365082 None:intergenic 45.0%
CTTGAGAAAATCGAGCCACT+TGG - chr4_4:56365064-56365083 Msa0689330:CDS 45.0%
ACATGCTTATGGTCTGACTG+AGG - chr4_4:56365099-56365118 Msa0689330:CDS 45.0%
! ATGGAGAGTGTGGTAAGAGA+TGG - chr4_4:56365259-56365278 Msa0689330:CDS 45.0%
! TGGAGAGTGTGGTAAGAGAT+GGG - chr4_4:56365260-56365279 Msa0689330:CDS 45.0%
GAAGGGAAGTGGAATCATGA+TGG - chr4_4:56365306-56365325 Msa0689330:CDS 45.0%
AGGGAAGTGGAATCATGATG+GGG - chr4_4:56365308-56365327 Msa0689330:CDS 45.0%
GCAATGCCACATCCAAAATG+GGG - chr4_4:56365538-56365557 Msa0689330:intron 45.0%
! CTTTCACCCCATTTTGGATG+TGG + chr4_4:56365547-56365566 None:intergenic 45.0%
! CAAGGACTTTCACCCCATTT+TGG + chr4_4:56365553-56365572 None:intergenic 45.0%
GACACTAAGGAAGAATGAGG+AGG - chr4_4:56365582-56365601 Msa0689330:intron 45.0%
GACGAGATAGAGCCATAATC+TGG + chr4_4:56365834-56365853 None:intergenic 45.0%
CGAACCTCAGTCATCCATGA+AGG - chr4_4:56363822-56363841 Msa0689330:CDS 50.0%
GGTTCCTTCATGGATGACTG+AGG + chr4_4:56363829-56363848 None:intergenic 50.0%
! AATATTGAGAGCACGGAGGG+AGG + chr4_4:56363898-56363917 None:intergenic 50.0%
TAGATTGCGGTCGCTGATCT+AGG + chr4_4:56364113-56364132 None:intergenic 50.0%
TAGATCAGCGACCGCAATCT+AGG - chr4_4:56364112-56364131 Msa0689330:CDS 50.0%
! GATGGTGTTTAGGACAGCAC+CGG + chr4_4:56364375-56364394 None:intergenic 50.0%
!! GACGGTGGTTGATGGTGTTT+AGG + chr4_4:56364385-56364404 None:intergenic 50.0%
CATCAACCACCGTCTCAATG+CGG - chr4_4:56364391-56364410 Msa0689330:CDS 50.0%
CTATGTTGGCCGCATTGAGA+CGG + chr4_4:56364403-56364422 None:intergenic 50.0%
!! CTCAGAGTGTCGAAGAATGG+TGG + chr4_4:56364426-56364445 None:intergenic 50.0%
CACCATTCTTCGACACTCTG+AGG - chr4_4:56364424-56364443 Msa0689330:CDS 50.0%
TAAACACTCCCACCGGAATC+CGG - chr4_4:56364573-56364592 Msa0689330:CDS 50.0%
CGAGCTCGAATACGAGCAAA+TGG - chr4_4:56364601-56364620 Msa0689330:CDS 50.0%
CACTACCCATTTCCCAACCA+AGG + chr4_4:56364775-56364794 None:intergenic 50.0%
CCGGTGAATATACTAACCGG+TGG - chr4_4:56365019-56365038 Msa0689330:CDS 50.0%
! TTTTCTCAAGTAGGGACGCC+GGG + chr4_4:56365054-56365073 None:intergenic 50.0%
TATGGTCTGACTGAGGCAAC+AGG - chr4_4:56365106-56365125 Msa0689330:CDS 50.0%
ATGGTCTGACTGAGGCAACA+GGG - chr4_4:56365107-56365126 Msa0689330:CDS 50.0%
TGCCACTCACAAACAAGTGC+TGG + chr4_4:56365133-56365152 None:intergenic 50.0%
!! GGGGATGGTTGGTTTAGAAC+TGG - chr4_4:56365367-56365386 Msa0689330:intron 50.0%
GGACTGCTCAACCTACTAAG+TGG + chr4_4:56366072-56366091 None:intergenic 50.0%
!! TTTAGGACAGCACCGGCCAT+AGG + chr4_4:56364368-56364387 None:intergenic 55.0%
CGCATTGAGACGGTGGTTGA+TGG + chr4_4:56364393-56364412 None:intergenic 55.0%
!! CGCCTCAGAGTGTCGAAGAA+TGG + chr4_4:56364429-56364448 None:intergenic 55.0%
ACTTGGGGTGTAGCTGCAAG+AGG - chr4_4:56364848-56364867 Msa0689330:CDS 55.0%
TTTCTCAAGTAGGGACGCCG+GGG + chr4_4:56365053-56365072 None:intergenic 55.0%
GGCCAGCACTTGTTTGTGAG+TGG - chr4_4:56365128-56365147 Msa0689330:CDS 55.0%
! TCGATGCTGAAAGCGCGACA+AGG - chr4_4:56365187-56365206 Msa0689330:CDS 55.0%
! CGATGCTGAAAGCGCGACAA+GGG - chr4_4:56365188-56365207 Msa0689330:CDS 55.0%
! GATGCTGAAAGCGCGACAAG+GGG - chr4_4:56365189-56365208 Msa0689330:CDS 55.0%
GAAGCGACGGCGAAAGCTTT+CGG - chr4_4:56365346-56365365 Msa0689330:intron 55.0%
AAGCGACGGCGAAAGCTTTC+GGG - chr4_4:56365347-56365366 Msa0689330:intron 55.0%
CGAAAGCTTTCGGGGATGGT+TGG - chr4_4:56365356-56365375 Msa0689330:intron 55.0%
TGTTGGCCGCATTGAGACGG+TGG + chr4_4:56364400-56364419 None:intergenic 60.0%
CGTATTCGAGCTCGCCTAGC+CGG + chr4_4:56364595-56364614 None:intergenic 60.0%
TGGGGTGTAGCTGCAAGAGG+AGG - chr4_4:56364851-56364870 Msa0689330:CDS 60.0%
TTCTCAAGTAGGGACGCCGG+GGG + chr4_4:56365052-56365071 None:intergenic 60.0%
AGCGACGGCGAAAGCTTTCG+GGG - chr4_4:56365348-56365367 Msa0689330:intron 60.0%
ACGGCGAAAGCTTTCGGGGA+TGG - chr4_4:56365352-56365371 Msa0689330:intron 60.0%
ACTCCCACCGGAATCCGGCT+AGG - chr4_4:56364578-56364597 Msa0689330:CDS 65.0%
TCGCCTAGCCGGATTCCGGT+GGG + chr4_4:56364584-56364603 None:intergenic 65.0%
GAGCTCGCCTAGCCGGATTC+CGG + chr4_4:56364588-56364607 None:intergenic 65.0%
AGGAAGCGAGGCGGCGACAC+CGG + chr4_4:56363878-56363897 None:intergenic 70.0%
CACGGAGGGAGGAAGCGAGG+CGG + chr4_4:56363887-56363906 None:intergenic 70.0%
GAGCACGGAGGGAGGAAGCG+AGG + chr4_4:56363890-56363909 None:intergenic 70.0%
CTCGCCTAGCCGGATTCCGG+TGG + chr4_4:56364585-56364604 None:intergenic 70.0%
! AACCGGTGGAGCACCACCCC+CGG - chr4_4:56365033-56365052 Msa0689330:CDS 70.0%
TCAAGTAGGGACGCCGGGGG+TGG + chr4_4:56365049-56365068 None:intergenic 70.0%
! CGCCGGGGGTGGTGCTCCAC+CGG + chr4_4:56365038-56365057 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 56363513 56366162 56363513 ID=Msa0689330;Name=Msa0689330
chr4_4 mRNA 56363513 56366162 56363513 ID=Msa0689330-mRNA-1;Parent=Msa0689330;Name=Msa0689330-mRNA-1;_AED=0.01;_eAED=0.01;_QI=222|1|1|1|1|1|2|304|581
chr4_4 exon 56363513 56365364 56363513 ID=Msa0689330-mRNA-1:exon:14843;Parent=Msa0689330-mRNA-1
chr4_4 exon 56365743 56366162 56365743 ID=Msa0689330-mRNA-1:exon:14842;Parent=Msa0689330-mRNA-1
chr4_4 five_prime_UTR 56365941 56366162 56365941 ID=Msa0689330-mRNA-1:five_prime_utr;Parent=Msa0689330-mRNA-1
chr4_4 CDS 56365743 56365940 56365743 ID=Msa0689330-mRNA-1:cds;Parent=Msa0689330-mRNA-1
chr4_4 CDS 56363817 56365364 56363817 ID=Msa0689330-mRNA-1:cds;Parent=Msa0689330-mRNA-1
chr4_4 three_prime_UTR 56363513 56363816 56363513 ID=Msa0689330-mRNA-1:three_prime_utr;Parent=Msa0689330-mRNA-1
Gene Sequence

>Msa0689330

ATGGATAACCTACCAAAATGTCGAGCAAACTATACCATACTCACTCCTTTAACTTTCTTGATGAGAGCTTCTGCATCTTATGCAAACCGAACCTCAGTCATCCATGAAGGAACCCGTTTCACTTGGTCACAAACTTATGACCGGTGTCGCCGCCTCGCTTCCTCCCTCCGTGCTCTCAATATTGCAAAGAATGATGTTGTATCAGTTTTGGCACCAAACATTCCAGCAATGTATGAGATGCATTTTGCAGTACCTATGGCCGGTGCTGTCCTAAACACCATCAACCACCGTCTCAATGCGGCCAACATAGCCACCATTCTTCGACACTCTGAGGCGAAAGTCTTCTTCGTAGACTACGAATTCGTATCAAAAGCAAAAGAGGCCTTAAGATTACTCATGGAAGAAAAAGATCAAAAAGAACAATACTCATCACTCCCACTAGTGATTGTGATAGATGACATAAACACTCCCACCGGAATCCGGCTAGGCGAGCTCGAATACGAGCAAATGGTTCATCATGGTAATCCAAATTACCTTCCTGAAGAAATTCAAGATGAATGGTCACCAATTACATTGAATTACACATCAGGTACAACTTCAGAACCAAAAGGTGTTGTTTATAGCCACAGAGGTGCTTATTTAAGTACTTTGAGTTTAATCCTTGGTTGGGAAATGGGTAGTGAACCAGTTTATCTATGGACATTACCTATGTTCCATTGTAATGGTTGGACATTCACTTGGGGTGTAGCTGCAAGAGGAGGTACTAATATTTGTATCAGAAACACTGCAGCATCTGATATATATAGAGCCATAAATCTCTATAATGTGACACACATGTGTTGCGCACCCATTATTTTCAACATTATTTTAGGCGCGAAACCAAGCGAAAAGAGAGTCATAAAATCTCCGGTGAATATACTAACCGGTGGAGCACCACCCCCGGCGTCCCTACTTGAGAAAATCGAGCCACTTGGTTTTCATGTAACACATGCTTATGGTCTGACTGAGGCAACAGGGCCAGCACTTGTTTGTGAGTGGCAGAAGAAATGGAATGTGTTGCCGAAAAGAGAGCAATCGATGCTGAAAGCGCGACAAGGGGTTAGTGTTTTGACACTTGCTGATGTTGATGTGAAGAATCTTGAAACAATGGAGAGTGTGGTAAGAGATGGGAAAACAATGGGGGAGATTATGTTGAAGGGAAGTGGAATCATGATGGGGTATTTTAAAGATAAAGAAGCGACGGCGAAAGCTTTCGGGGATGGTTGGTTTAGAACTGGTGATGTTGGTGTTATACATAAAGATGGTTATTTGGAAATTAAAGATAGGTCTAAAGATGTGATTATTTCAGGAGGTGAAAATATTAGTAGTGTTGAAGTTGAAAATGTTCTTTATAGTCATCCTAAGGTTTTAGAAGCTGCTGTTGTTGCAATGCCACATCCAAAATGGGGTGAAAGTCCTTGTGCTTTTGTGACACTAAGGAAGAATGAGGAGGAAAAAAGTGATTTTTGTTGTGTTACTGAGGATGAGATAATTACCTATTGTAGGAAGAATTTACCACACTTTATGGTACCTAAGATGGTTGTTTTTATGGAGGATTTACCAAAGACTTTAACAGGTAAGATTCAGAAATTTGAATTAAGAGCCAAAGCTAAGTGTTTTGTGGTAAATGATGAGAAGAATAAGAAGAAGCCTAATAATCAAGTCAATCATAATAATGACCAGATTATGGCTCTATCTCGTCTTTGA
Protein sequence

>Msa0689330

MDNLPKCRANYTILTPLTFLMRASASYANRTSVIHEGTRFTWSQTYDRCRRLASSLRALNIAKNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINHRLNAANIATILRHSEAKVFFVDYEFVSKAKEALRLLMEEKDQKEQYSSLPLVIVIDDINTPTGIRLGELEYEQMVHHGNPNYLPEEIQDEWSPITLNYTSGTTSEPKGVVYSHRGAYLSTLSLILGWEMGSEPVYLWTLPMFHCNGWTFTWGVAARGGTNICIRNTAASDIYRAINLYNVTHMCCAPIIFNIILGAKPSEKRVIKSPVNILTGGAPPPASLLEKIEPLGFHVTHAYGLTEATGPALVCEWQKKWNVLPKREQSMLKARQGVSVLTLADVDVKNLETMESVVRDGKTMGEIMLKGSGIMMGYFKDKEATAKAFGDGWFRTGDVGVIHKDGYLEIKDRSKDVIISGGENISSVEVENVLYSHPKVLEAAVVAMPHPKWGESPCAFVTLRKNEEEKSDFCCVTEDEIITYCRKNLPHFMVPKMVVFMEDLPKTLTGKIQKFELRAKAKCFVVNDEKNKKKPNNQVNHNNDQIMALSRL*