AlfalfaGEDB | Msa0094420

M. sativa cultivar zhongmu-4 / Msa0094420

KEGG: -
GO: -
PFAM: PF14226.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0094420	XP_004513523.1	72.727	374	98	2	1	374	2	371	0.0	566

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0094420	sp\|Q9LTH7\|ACH12_ARATH	53.168	363	160	6	13	371	5	361	3.92e-138	403

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0094420	A0A1S2Z1X3	72.727	374	98	2	1	374	2	371	0.0	566

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0094420	0.815869	7.410639e-52	-8.615850e-47
Msa0001460	Msa0094420	0.828125	1.069524e-54	-8.615850e-47
Msa0001950	Msa0094420	0.817089	3.950546e-52	-8.615850e-47
Msa0003470	Msa0094420	0.899579	1.722928e-77	-8.615850e-47
Msa0004350	Msa0094420	0.845192	4.732664e-59	-8.615850e-47
Msa0005710	Msa0094420	0.802415	5.670926e-49	-8.615850e-47
Msa0006700	Msa0094420	0.822113	2.817434e-53	-8.615850e-47
Msa0006820	Msa0094420	0.819677	1.024149e-52	-8.615850e-47
Msa0007160	Msa0094420	0.825547	4.418017e-54	-8.615850e-47
Msa0007230	Msa0094420	0.808190	3.497767e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0094420	MtrunA17_Chr8g0364471	65.775	374	125	2	1	374	1	371	0.0	514
Msa0094420	MtrunA17_Chr7g0235951	63.369	374	134	2	1	374	1	371	5.88e-178	502
Msa0094420	MtrunA17_Chr8g0335411	65.241	374	127	2	1	374	1	371	1.03e-175	496
Msa0094420	MtrunA17_Chr7g0236011	64.706	374	129	2	1	374	1	371	2.49e-170	483
Msa0094420	MtrunA17_Chr7g0247991	64.171	374	131	2	1	374	1	371	1.54e-168	478
Msa0094420	MtrunA17_Chr1g0187821	63.807	373	123	7	8	374	2	368	4.33e-167	474
Msa0094420	MtrunA17_Chr4g0066991	65.067	375	106	4	1	374	1	351	1.08e-165	470
Msa0094420	MtrunA17_Chr1g0187811	59.103	379	139	7	8	374	117	491	2.00e-157	454
Msa0094420	MtrunA17_Chr2g0311701	58.355	377	150	5	1	374	1	373	3.82e-157	449
Msa0094420	MtrunA17_Chr1g0187831	58.378	370	143	6	11	374	8	372	6.70e-155	444

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0094420	AT5G59540.1	53.168	363	160	6	13	371	5	361	3.99e-139	403
Msa0094420	AT5G59530.1	53.951	367	156	8	13	374	4	362	1.03e-136	397
Msa0094420	AT1G04350.1	52.514	358	159	4	17	371	6	355	6.81e-136	395
Msa0094420	AT1G06620.1	52.793	358	162	4	15	371	9	360	1.13e-135	394
Msa0094420	AT2G30840.1	52.893	363	165	2	14	374	2	360	8.75e-131	382
Msa0094420	AT1G06650.2	49.591	367	173	4	14	374	7	367	1.17e-128	377
Msa0094420	AT1G06640.1	50.546	366	169	4	16	375	9	368	3.10e-126	370
Msa0094420	AT5G43450.1	50.000	362	170	4	13	371	4	357	1.61e-125	369
Msa0094420	AT2G30840.3	51.791	363	163	3	14	374	2	354	5.90e-125	367
Msa0094420	AT1G03410.2	50.712	351	163	6	16	362	3	347	3.69e-124	365

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Find 83 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCATGCAAAGATTGG+GGG	0.645357	1_2:+77618792	Msa0094420:CDS
AAACTAGCTACCGAAACTCT+TGG	0.537755	1_2:-77620082	None:intergenic
AAAGAATTGAGTTGTGCTGA+AGG	0.563547	1_2:+77619477	Msa0094420:CDS
AAAGATTTATGGTCCTATCA+AGG	0.521209	1_2:+77620141	Msa0094420:CDS
AAATAATACCTAAATACTTG+AGG	0.619921	1_2:-77619049	None:intergenic
AACCACAGTGATCCGCATGA+CGG	0.679578	1_2:-77620919	None:intergenic
AACCTATTTGCTCCGTCATG+CGG	0.535468	1_2:+77620907	Msa0094420:CDS
AACTCTGGTTCAGGACATGG+CGG	0.690066	1_2:-77619525	None:intergenic
AAGAAAATCCACCAGTCTAT+AGG	0.322522	1_2:+77620176	Msa0094420:CDS
AAGAATTGCCTCAAGTATTT+AGG	0.231549	1_2:+77619041	Msa0094420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATTCCATATTGTGTTAG+AGG	+	chr1_2:77618456-77618475	Msa0094420:five_prime_UTR	30.0%
AAAAATGCATGCAAAGATTG+GGG	+	chr1_2:77618791-77618810	Msa0094420:CDS	30.0%
TGGAATTCCTATTAATGTCT+TGG	+	chr1_2:77618835-77618854	Msa0094420:CDS	30.0%
ATCTCATCCAAGACATTAAT+AGG	-	chr1_2:77618845-77618864	None:intergenic	30.0%
AGATGATTAATGGAATTCGT+AGG	+	chr1_2:77618861-77618880	Msa0094420:CDS	30.0%
AAGAATTGCCTCAAGTATTT+AGG	+	chr1_2:77619041-77619060	Msa0094420:CDS	30.0%
TTGATATCTTAAGAGCTCTA+GGG	-	chr1_2:77619214-77619233	None:intergenic	30.0%
TTTGATATCTTAAGAGCTCT+AGG	-	chr1_2:77619215-77619234	None:intergenic	30.0%
TCCAAGAAAATAATGTCACT+CGG	+	chr1_2:77619399-77619418	Msa0094420:CDS	30.0%
AAGTTCTTCATGAGAATCAA+TGG	+	chr1_2:77619622-77619641	Msa0094420:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	77618382	77621013	77618382	ID=Msa0094420;Name=Msa0094420
chr1_2	mRNA	77618382	77621013	77618382	ID=Msa0094420-mRNA-1;Parent=Msa0094420;Name=Msa0094420-mRNA-1;_AED=0.22;_eAED=0.22;_QI=136\|0.66\|0.5\|1\|1\|1\|4\|0\|444
chr1_2	exon	77618382	77619062	77618382	ID=Msa0094420-mRNA-1:exon:27527;Parent=Msa0094420-mRNA-1
chr1_2	exon	77619380	77619701	77619380	ID=Msa0094420-mRNA-1:exon:27528;Parent=Msa0094420-mRNA-1
chr1_2	exon	77620019	77620275	77620019	ID=Msa0094420-mRNA-1:exon:27529;Parent=Msa0094420-mRNA-1
chr1_2	exon	77620803	77621013	77620803	ID=Msa0094420-mRNA-1:exon:27530;Parent=Msa0094420-mRNA-1
chr1_2	five_prime_UTR	77618382	77618517	77618382	ID=Msa0094420-mRNA-1:five_prime_utr;Parent=Msa0094420-mRNA-1
chr1_2	CDS	77618518	77619062	77618518	ID=Msa0094420-mRNA-1:cds;Parent=Msa0094420-mRNA-1
chr1_2	CDS	77619380	77619701	77619380	ID=Msa0094420-mRNA-1:cds;Parent=Msa0094420-mRNA-1
chr1_2	CDS	77620019	77620275	77620019	ID=Msa0094420-mRNA-1:cds;Parent=Msa0094420-mRNA-1
chr1_2	CDS	77620803	77621013	77620803	ID=Msa0094420-mRNA-1:cds;Parent=Msa0094420-mRNA-1

Gene Sequence

>Msa0094420

ATGATGGTGAAACCAACAAACCAATTAGAGGAAGCTAATGATTCCAGTTATGATAGAAAAGTTGAAGTGACAACCTTTGATGAATCGAAAGCTGGTGTAAGAGGTCTTATAGAGTCCGGTGTAACAAAGATTCCACGCATGTTCTACCGTGACACATTGAACAACACTGAACATTTAGTCAATCGATCCAACTTGAGTATTCCGGTCATAGATCTCAAAGACATACACAACAATATCAATCATGCTATGCGTATTGAGGTAATCGGCCAACTTAAAAATGCATGCAAAGATTGGGGGTTCTTCCAGGTGATTAATCATGGAATTCCTATTAATGTCTTGGATGAGATGATTAATGGAATTCGTAGGTTTCATGAACAAGACGTTGTTGAAAGGAAAAAATTTTACAACCGTGATTTGACAAAGAAAATTATTTATTATTCCAATATTGGTTTGTATGTAGAAAAATCTGCTATTTGGAGAGACTCTTTTGGATGTACCATGGCTCCTAATCCACCAAAACCAGAAGAATTGCCTCAAGTATTTAGAGACATTATAATAGAATATTCCAAGAAAATAATGTCACTCGGATCTACACTTTTTGAGTTATTGTCAGAGGCTCTTGGCTTGAATTCATCTTACCTAAAAGAATTGAGTTGTGCTGAAGGACTTTTTATTCAAGGTCATTATTATCCGCCATGTCCTGAACCAGAGTTAACTATGGGTACAGACAAGCACGCTGATTCTAGCTTCATGACAATACTTTTACAAGACCAAATTGGTGGTCTTCAAGTTCTTCATGAGAATCAATGGGTTAATGTTCCTCCTCTACATGGAGCTCTTGTTGTCAACATAGGAGATCTTTTACAACTTATAACAAATGACAATTTCGTCAGTGTTTATCATCGGGTCTTGCCACCAAGTCATAAAGGACCAAGAGTTTCGGTAGCTAGTTTCTTTGCAAATTCTCATGATCCAACTGAAGTTTCATCAAAGATTTATGGTCCTATCAAGGAACTGACATCAGAAGAAAATCCACCAGTCTATAGGGACATAAAAGTTGCAGAATACATGGACAATCGTTTTAAGAAGGGGCATGATGGAAGCTCTAAGTTGGAGCCTTTCAACTCTCTAGTGGGTGTTGAAGATTGTGGTCTCGTTTTGTTACTGACACAAGGTGGTGTACACTTTCAGCGTCACCTTCTGAGACTACTTCAAGTGGCCGTCGACGAACCTATTTGCTCCGTCATGCGGATCACTGTGGTTCTTTTAAGATATGTGGTTTCTCGACCCATATATGTGGTTTCTAGTTACGAAGGTTGGAGTGAGTTCAGTTGA

Protein sequence

>Msa0094420

MMVKPTNQLEEANDSSYDRKVEVTTFDESKAGVRGLIESGVTKIPRMFYRDTLNNTEHLVNRSNLSIPVIDLKDIHNNINHAMRIEVIGQLKNACKDWGFFQVINHGIPINVLDEMINGIRRFHEQDVVERKKFYNRDLTKKIIYYSNIGLYVEKSAIWRDSFGCTMAPNPPKPEELPQVFRDIIIEYSKKIMSLGSTLFELLSEALGLNSSYLKELSCAEGLFIQGHYYPPCPEPELTMGTDKHADSSFMTILLQDQIGGLQVLHENQWVNVPPLHGALVVNIGDLLQLITNDNFVSVYHRVLPPSHKGPRVSVASFFANSHDPTEVSSKIYGPIKELTSEENPPVYRDIKVAEYMDNRFKKGHDGSSKLEPFNSLVGVEDCGLVLLLTQGGVHFQRHLLRLLQVAVDEPICSVMRITVVLLRYVVSRPIYVVSSYEGWSEFS*