AlfalfaGEDB | Msa0541580

M. sativa cultivar zhongmu-4 / Msa0541580

KEGG: K17785
GO: -
PFAM: PF09731.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0541580	XP_024629043.1	94.290	648	34	1	1	648	1	645	0.0	1211

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0541580	A0A396GPD9	94.290	648	34	1	1	648	1	645	0.0	1211

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006110	Msa0541580	0.820303	7.365061e-53	-8.615850e-47
Msa0027320	Msa0541580	0.800183	1.623897e-48	-8.615850e-47
Msa0105070	Msa0541580	0.804418	2.181367e-49	-8.615850e-47
Msa0109900	Msa0541580	0.801693	7.980975e-49	-8.615850e-47
Msa0225750	Msa0541580	0.801906	7.217024e-49	-8.615850e-47
Msa0244860	Msa0541580	0.802233	6.183775e-49	-8.615850e-47
Msa0244940	Msa0541580	0.800385	1.477318e-48	-8.615850e-47
Msa0245940	Msa0541580	0.813259	2.802069e-51	-8.615850e-47
Msa0248970	Msa0541580	0.802792	4.741879e-49	-8.615850e-47
Msa0283450	Msa0541580	0.812774	3.579707e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0541580	MtrunA17_Chr8g0382781	94.290	648	34	1	1	648	1	645	0.0	1211

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0541580	AT4G39690.1	46.188	682	301	16	1	648	1	650	3.41e-170	501

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Find 175 sgRNAs with CRISPR-Local

Find 375 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGAAAAGAAGTTGA+AGG	0.524724	4_1:-25560902	Msa0541580:CDS
AAAATGTTGCATTGTTAGTA+TGG	0.218900	4_1:-25557206	Msa0541580:three_prime_UTR
AAACAGGGCTATTTCTGAGC+AGG	0.515557	4_1:-25557514	Msa0541580:CDS
AAAGATAGAACTTGAACAAA+AGG	0.443411	4_1:-25560328	Msa0541580:intron
AAATAATCTCTCCAACCTTT+AGG	0.317282	4_1:+25557400	None:intergenic
AAATTTCAATAATATACCCG+CGG	0.709693	4_1:-25557183	Msa0541580:three_prime_UTR
AACAAGAAATAGAGGAAAGA+AGG	0.410076	4_1:-25561636	Msa0541580:CDS
AACCTTGTCTTGGTCGCCTG+TGG	0.539457	4_1:+25557160	None:intergenic
AAGAAACTGCAATGTTGGAT+AGG	0.496092	4_1:-25560849	Msa0541580:CDS
AAGCTGACCAATCAGGTGAT+GGG	0.584412	4_1:-25557671	Msa0541580:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCGAGATGAAAATTGCAAAA+TGG	+	chr4_1:25557387-25557406	None:intergenic	30.0%
CCTGATTACTAAAATATCTG+GGG	+	chr4_1:25557430-25557449	None:intergenic	30.0%
ACTTCAGAAGAGACATTTAA+AGG	+	chr4_1:25557682-25557701	None:intergenic	30.0%
ATGTGAAACTGACGAAAATT+TGG	+	chr4_1:25557811-25557830	None:intergenic	30.0%
CTTAGATAAATACGTTGAGA+AGG	-	chr4_1:25558060-25558079	Msa0541580:intron	30.0%
GAATCTTCTATTGCAATTTC+TGG	+	chr4_1:25558253-25558272	None:intergenic	30.0%
AACAAGAAATAGAGGAAAGA+AGG	-	chr4_1:25558551-25558570	Msa0541580:CDS	30.0%
ACAAGAAATAGAGGAAAGAA+GGG	-	chr4_1:25558552-25558571	Msa0541580:CDS	30.0%
TTAACTAATACATTCTGCCA+TGG	+	chr4_1:25558787-25558806	None:intergenic	30.0%
ATTGCAAAAGACAATAACAC+AGG	-	chr4_1:25559440-25559459	Msa0541580:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	25557041	25563168	25557041	ID=Msa0541580;Name=Msa0541580
chr4_1	mRNA	25557041	25563168	25557041	ID=Msa0541580-mRNA-1;Parent=Msa0541580;Name=Msa0541580-mRNA-1;_AED=0.06;_eAED=0.06;_QI=169\|0.90\|0.91\|1\|1\|1\|12\|426\|648
chr4_1	exon	25557041	25557700	25557041	ID=Msa0541580-mRNA-1:exon:6885;Parent=Msa0541580-mRNA-1
chr4_1	exon	25557905	25558006	25557905	ID=Msa0541580-mRNA-1:exon:6884;Parent=Msa0541580-mRNA-1
chr4_1	exon	25558429	25558617	25558429	ID=Msa0541580-mRNA-1:exon:6883;Parent=Msa0541580-mRNA-1
chr4_1	exon	25558710	25558787	25558710	ID=Msa0541580-mRNA-1:exon:6882;Parent=Msa0541580-mRNA-1
chr4_1	exon	25560103	25560222	25560103	ID=Msa0541580-mRNA-1:exon:6881;Parent=Msa0541580-mRNA-1
chr4_1	exon	25560329	25560421	25560329	ID=Msa0541580-mRNA-1:exon:6880;Parent=Msa0541580-mRNA-1
chr4_1	exon	25560849	25560926	25560849	ID=Msa0541580-mRNA-1:exon:6879;Parent=Msa0541580-mRNA-1
chr4_1	exon	25561040	25561192	25561040	ID=Msa0541580-mRNA-1:exon:6878;Parent=Msa0541580-mRNA-1
chr4_1	exon	25561291	25561416	25561291	ID=Msa0541580-mRNA-1:exon:6877;Parent=Msa0541580-mRNA-1
chr4_1	exon	25561633	25562328	25561633	ID=Msa0541580-mRNA-1:exon:6876;Parent=Msa0541580-mRNA-1
chr4_1	exon	25562761	25562827	25562761	ID=Msa0541580-mRNA-1:exon:6875;Parent=Msa0541580-mRNA-1
chr4_1	exon	25562989	25563168	25562989	ID=Msa0541580-mRNA-1:exon:6874;Parent=Msa0541580-mRNA-1
chr4_1	five_prime_UTR	25563000	25563168	25563000	ID=Msa0541580-mRNA-1:five_prime_utr;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25562989	25562999	25562989	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25562761	25562827	25562761	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25561633	25562328	25561633	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25561291	25561416	25561291	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25561040	25561192	25561040	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25560849	25560926	25560849	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25560329	25560421	25560329	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25560103	25560222	25560103	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25558710	25558787	25558710	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25558429	25558617	25558429	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25557905	25558006	25557905	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	CDS	25557467	25557700	25557467	ID=Msa0541580-mRNA-1:cds;Parent=Msa0541580-mRNA-1
chr4_1	three_prime_UTR	25557041	25557466	25557041	ID=Msa0541580-mRNA-1:three_prime_utr;Parent=Msa0541580-mRNA-1

Gene Sequence

>Msa0541580

ATGTTCCGGAGGTCCATTTTGCAATTTTCATCTCGAAAACAATCCTTTAGAACAAACCCCAGATATTTTAGTAATCAGAAGATACCTTCACATCTATCTTCTCGAAAGAAATTCTCAAATGCATCGAAACCTGGTGGTGCCTCAGCTCCTTATTCTACAGGAAAGCCGCCCGAGTCTCATGGTTCTAAGTCAAAGTCTTTTTTAATTGGAGGTGCTGGTGTAAGTGCTGTTCTTCTGGCAGCTTATCAATTTGGTTTCTTAGATAAATACGTTGAGAAGGAGAAGCTTAGTATTCCCCAAGAAGCTCAGATAGATGGTACCGTTGGAGATTTGGAGAGTGGACAACATTCAATAGAGGAGTTAATTTCACTCACTAGTGAGAAATCTAACAATGAAAATCCTGTTGTAGAGCATGCTGAGCAGAAGGTTGATACCCATCTTTCACAGCCAGAAATTGCAATAGAAGATTCAAGTGATAAACCGATTCCTGTGCAAGACACTTCTGATATTGCTGAAGATCATAATGCTGGTGCAAAAGAAAACCAGTTTCCCGAATATCCTCAAAGTAGTCTATCATCTGATGATCCTAGTAAAGAGAGTGTGGTGCAATCTGATGGAACTGTTGGCATAAAAAGCACCGAAGCAGATGTTACCCTAAAACCGGAAGAGGCAATTCAACATACATCGCCATCTACACAGGACAATACATTTCTAGATGAGAAGGGGACAGAAAATATTCAACCAAAGCAACAAGAAATAGAGGAAAGAAGGGAGAATGTATTAGTTAAAGATATAGAACAACCACCTACCCTCCTTGAGGAATATCACATAAGGAATAAGTCGGAAGGAACCGCTCCTATTGGGCTTACCGAGAACAGTCATTTTCCTGAAGAAAAAGAGGCATTTAGTGGTGCAACGGAGGACTTGAAAGATGGTTATGTGACTAAGGATGGAAAAGTGGTTCTTGATTTCGTACAAGCCATCCATGCTGCGGAGAAAAGGCAAGCTGGAATAGATGCACATGCTTTTAATGAAGAGAAGAAAGCATTGAAGGAAAAATATGAAAAGAAGTTGAAGGATGCAGCTGCCAAAGAACTAATGCTTGCTGAAGAAACTGCAATGTTGGATAGGGAGCTTAAGAGAGAGAGGGCAAAAGCAGCTCTTGCTATTAAGTCACTTCAAGAAAAAATGGACGAGAAACTAAAGATAGAACTTGAACAAAAGAAAATCGAAGCAGAAGAAAGTTTGAAGAAAAATCAGGAGTTAGCACAGGCAGAGTTAAATGCAGCCATAGCAAAGGAGAAGGCAGCCCAGCTTGAAAAAATGTCTGAAGCAAATATTAATATAAATGCCTTGTGCATGGCATTTTATGCTCGATCTGAAGAAGCTCGTCAAAGTCATGCTGCACAAAGTTTTGCTTTGAGAGCACTTGCACTTGAAGATGCACTGTCAAAAGGATTGCCAATTCAGACAGAATTAGAATCTTTGCAGTCTTATCTTGAAGGCACAGATAAAGATTCAGTGTTGGATTTGGTTCTAGCGTCCCTTCTTGAAGAGACAAGAAACAACGGCACGGACACACATCTGCAATTAAAGCAGAAGGCAAGCCGTTTTGATATCATAAAAGGTAATGTACGACACTTCGTCTTCTTCCCACCAGGAGGGGGAGGTATGTTGGCACATTCTTTGGCGCGTATAGCATCCTTGTTGAAGGTTAAGGAAGCTGACCAATCAGGTGATGGGATTGAATCTGTTATTAACAAAGTTGAGAGTTACTTGGCAGAAGGAAAACTTGCTGAAGCAGCAGATTGTCTAGAAGAAAGTGTGAGGGATACCCAAGCTGCAGAAATTGTTGCAGGTTGGGTGAAGCAAGCAAGAAACAGGGCTATTTCTGAGCAGGCTGTAGTTTTTCTTCAATCCTATGCCAATTCCATTAGCCTTACATGA

Protein sequence

>Msa0541580

MFRRSILQFSSRKQSFRTNPRYFSNQKIPSHLSSRKKFSNASKPGGASAPYSTGKPPESHGSKSKSFLIGGAGVSAVLLAAYQFGFLDKYVEKEKLSIPQEAQIDGTVGDLESGQHSIEELISLTSEKSNNENPVVEHAEQKVDTHLSQPEIAIEDSSDKPIPVQDTSDIAEDHNAGAKENQFPEYPQSSLSSDDPSKESVVQSDGTVGIKSTEADVTLKPEEAIQHTSPSTQDNTFLDEKGTENIQPKQQEIEERRENVLVKDIEQPPTLLEEYHIRNKSEGTAPIGLTENSHFPEEKEAFSGATEDLKDGYVTKDGKVVLDFVQAIHAAEKRQAGIDAHAFNEEKKALKEKYEKKLKDAAAKELMLAEETAMLDRELKRERAKAALAIKSLQEKMDEKLKIELEQKKIEAEESLKKNQELAQAELNAAIAKEKAAQLEKMSEANININALCMAFYARSEEARQSHAAQSFALRALALEDALSKGLPIQTELESLQSYLEGTDKDSVLDLVLASLLEETRNNGTDTHLQLKQKASRFDIIKGNVRHFVFFPPGGGGMLAHSLARIASLLKVKEADQSGDGIESVINKVESYLAEGKLAEAADCLEESVRDTQAAEIVAGWVKQARNRAISEQAVVFLQSYANSISLT*