AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1306960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306960 MtrunA17_Chr8g0345171 96.269 134 4 1 1 134 1 133 6.33e-94 266
Msa1306960 MtrunA17_Chr7g0276621 55.469 128 43 6 1 123 23 141 1.01e-37 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306960 AT3G14280.1 39.815 108 57 3 21 128 21 120 2.84e-15 68.2

Find 29 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTTGAATTGCAATAAAA+TGG 0.221285 8_4:+1919672 Msa1306960:exon
TTCTTGAAGAATTAGCTTAA+TGG 0.238281 8_4:+1918637 Msa1306960:exon
GAATTATCATATTTCTGAAT+TGG 0.275390 8_4:-1918689 None:intergenic
AAGAAGAAGATGAGATAATA+AGG 0.331139 8_4:-1918600 None:intergenic
AATTATCATATTTCTGAATT+GGG 0.345181 8_4:-1918688 None:intergenic
TTGTCTCACTCATAGCTTTG+AGG 0.351441 8_4:+1919725 Msa1306960:three_prime_UTR
AGTTGGTTTCAAATTGCAAA+AGG 0.405294 8_4:+1919602 Msa1306960:CDS
CTTTGGAGCATAGCATCGTT+TGG 0.411384 8_4:-1918744 None:intergenic
TGAATCTTCTAAAACAAAGT+TGG 0.441522 8_4:+1919585 Msa1306960:CDS
CTTGAAATTTGTCTATAAGA+TGG 0.456782 8_4:-1919264 None:intergenic
TCAAATAGCACATGTGTAGT+AGG 0.464126 8_4:+1919794 Msa1306960:three_prime_UTR
TTATTTCTCCACTCTCATCT+TGG 0.480024 8_4:-1919218 None:intergenic
GTTGGTTTCAAATTGCAAAA+GGG 0.500062 8_4:+1919603 Msa1306960:CDS
ATTTGTGAAGGGTATGATGA+AGG 0.501766 8_4:+1919240 Msa1306960:CDS
GACCAACGGTTCATCACAAA+AGG 0.504204 8_4:+1919286 Msa1306960:CDS
GAAATTGATGATGCAATTGA+AGG 0.528412 8_4:+1919629 Msa1306960:CDS
GTGGAGAAATAATTTGTGAA+GGG 0.550320 8_4:+1919229 Msa1306960:CDS
AGTGGAGAAATAATTTGTGA+AGG 0.561503 8_4:+1919228 Msa1306960:CDS
TTTGTGAAGGGTATGATGAA+GGG 0.566441 8_4:+1919241 Msa1306960:CDS
CACAGCATCCATCAAGAACA+AGG 0.570428 8_4:+1918802 Msa1306960:CDS
ATTCTTCAAGAACAAGAAAG+AGG 0.573168 8_4:-1918626 None:intergenic
AAGAAGATGAGATAATAAGG+AGG 0.575986 8_4:-1918597 None:intergenic
TAGACAAATTTCAAGACCAA+CGG 0.578411 8_4:+1919272 Msa1306960:CDS
TGAAGGTGGTCGTTTGCAAG+AGG 0.580771 8_4:+1919646 Msa1306960:CDS
AAAGTCAACAGTTGGTCCAG+TGG 0.602548 8_4:+1918769 Msa1306960:CDS
CAAAGCTATGAGTGAGACAA+AGG 0.622287 8_4:-1919722 None:intergenic
ATTTGCTACCAAGATGAGAG+TGG 0.633835 8_4:+1919210 Msa1306960:CDS
CTTCAAGAACAAGAAAGAGG+TGG 0.639571 8_4:-1918623 None:intergenic
ATTGATGATGCAATTGAAGG+TGG 0.706142 8_4:+1919632 Msa1306960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATCATATTTCTGAATT+GGG - chr8_4:1918691-1918710 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG - chr8_4:1918835-1918854 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG - chr8_4:1918878-1918897 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG + chr8_4:1918924-1918943 Msa1306960:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG + chr8_4:1919044-1919063 Msa1306960:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG - chr8_4:1919370-1919389 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG + chr8_4:1919371-1919390 Msa1306960:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG + chr8_4:1919672-1919691 Msa1306960:exon 15.0%
!!! AATTATCATATTTCTGAATT+GGG - chr8_4:1918691-1918710 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG - chr8_4:1918835-1918854 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG - chr8_4:1918878-1918897 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG + chr8_4:1918924-1918943 Msa1306960:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG + chr8_4:1919044-1919063 Msa1306960:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG - chr8_4:1919370-1919389 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG + chr8_4:1919371-1919390 Msa1306960:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG + chr8_4:1919672-1919691 Msa1306960:exon 15.0%
!!! GAATTATCATATTTCTGAAT+TGG - chr8_4:1918692-1918711 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG + chr8_4:1918934-1918953 Msa1306960:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG - chr8_4:1919012-1919031 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG + chr8_4:1919064-1919083 Msa1306960:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG + chr8_4:1919334-1919353 Msa1306960:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG + chr8_4:1919529-1919548 Msa1306960:intron 20.0%
!!! GAATTATCATATTTCTGAAT+TGG - chr8_4:1918692-1918711 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG + chr8_4:1918934-1918953 Msa1306960:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG - chr8_4:1919012-1919031 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG + chr8_4:1919064-1919083 Msa1306960:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG + chr8_4:1919334-1919353 Msa1306960:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG + chr8_4:1919529-1919548 Msa1306960:intron 20.0%
! AATAAGGAGGAAAAATGAAA+TGG - chr8_4:1918587-1918606 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG - chr8_4:1918603-1918622 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG + chr8_4:1918637-1918656 Msa1306960:exon 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG + chr8_4:1918942-1918961 Msa1306960:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG + chr8_4:1918981-1919000 Msa1306960:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG - chr8_4:1919013-1919032 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG + chr8_4:1919100-1919119 Msa1306960:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG + chr8_4:1919158-1919177 Msa1306960:intron 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG + chr8_4:1919170-1919189 Msa1306960:intron 25.0%
! CTTGAAATTTGTCTATAAGA+TGG - chr8_4:1919267-1919286 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG + chr8_4:1919585-1919604 Msa1306960:CDS 25.0%
! AATAAGGAGGAAAAATGAAA+TGG - chr8_4:1918587-1918606 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG - chr8_4:1918603-1918622 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG + chr8_4:1918637-1918656 Msa1306960:exon 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG + chr8_4:1918942-1918961 Msa1306960:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG + chr8_4:1918981-1919000 Msa1306960:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG - chr8_4:1919013-1919032 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG + chr8_4:1919100-1919119 Msa1306960:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG + chr8_4:1919158-1919177 Msa1306960:intron 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG + chr8_4:1919170-1919189 Msa1306960:intron 25.0%
! CTTGAAATTTGTCTATAAGA+TGG - chr8_4:1919267-1919286 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG + chr8_4:1919585-1919604 Msa1306960:CDS 25.0%
AAGAAGATGAGATAATAAGG+AGG - chr8_4:1918600-1918619 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG - chr8_4:1918629-1918648 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG - chr8_4:1918813-1918832 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG + chr8_4:1919007-1919026 Msa1306960:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG + chr8_4:1919021-1919040 Msa1306960:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG + chr8_4:1919228-1919247 Msa1306960:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG + chr8_4:1919229-1919248 Msa1306960:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG + chr8_4:1919272-1919291 Msa1306960:CDS 30.0%
! ATGACAATTTTATTCTTCGG+CGG + chr8_4:1919374-1919393 Msa1306960:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG + chr8_4:1919402-1919421 Msa1306960:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG + chr8_4:1919430-1919449 Msa1306960:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG + chr8_4:1919441-1919460 Msa1306960:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG + chr8_4:1919467-1919486 Msa1306960:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG - chr8_4:1919548-1919567 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG + chr8_4:1919549-1919568 Msa1306960:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG + chr8_4:1919550-1919569 Msa1306960:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG + chr8_4:1919602-1919621 Msa1306960:CDS 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG + chr8_4:1919603-1919622 Msa1306960:CDS 30.0%
!! GAAATTGATGATGCAATTGA+AGG + chr8_4:1919629-1919648 Msa1306960:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG - chr8_4:1919826-1919845 None:intergenic 30.0%
AAGAAGATGAGATAATAAGG+AGG - chr8_4:1918600-1918619 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG - chr8_4:1918629-1918648 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG - chr8_4:1918813-1918832 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG + chr8_4:1919007-1919026 Msa1306960:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG + chr8_4:1919021-1919040 Msa1306960:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG + chr8_4:1919228-1919247 Msa1306960:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG + chr8_4:1919229-1919248 Msa1306960:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG + chr8_4:1919272-1919291 Msa1306960:CDS 30.0%
! ATGACAATTTTATTCTTCGG+CGG + chr8_4:1919374-1919393 Msa1306960:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG + chr8_4:1919402-1919421 Msa1306960:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG + chr8_4:1919430-1919449 Msa1306960:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG + chr8_4:1919441-1919460 Msa1306960:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG + chr8_4:1919467-1919486 Msa1306960:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG - chr8_4:1919548-1919567 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG + chr8_4:1919549-1919568 Msa1306960:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG + chr8_4:1919550-1919569 Msa1306960:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG + chr8_4:1919602-1919621 Msa1306960:CDS 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG + chr8_4:1919603-1919622 Msa1306960:CDS 30.0%
!! GAAATTGATGATGCAATTGA+AGG + chr8_4:1919629-1919648 Msa1306960:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG - chr8_4:1919826-1919845 None:intergenic 30.0%
!!! CCAACTGTTGACTTTTTCTT+TGG - chr8_4:1918764-1918783 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG + chr8_4:1918761-1918780 Msa1306960:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG - chr8_4:1919108-1919127 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG + chr8_4:1919105-1919124 Msa1306960:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG + chr8_4:1919183-1919202 Msa1306960:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG - chr8_4:1919221-1919240 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG + chr8_4:1919240-1919259 Msa1306960:CDS 35.0%
! TTTGTGAAGGGTATGATGAA+GGG + chr8_4:1919241-1919260 Msa1306960:CDS 35.0%
TACCAGTTAAAATCTTTCGG+CGG + chr8_4:1919405-1919424 Msa1306960:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG + chr8_4:1919632-1919651 Msa1306960:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG + chr8_4:1919794-1919813 Msa1306960:three_prime_UTR 35.0%
!!! CCAACTGTTGACTTTTTCTT+TGG - chr8_4:1918764-1918783 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG + chr8_4:1918761-1918780 Msa1306960:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG - chr8_4:1919108-1919127 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG + chr8_4:1919105-1919124 Msa1306960:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG + chr8_4:1919183-1919202 Msa1306960:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG - chr8_4:1919221-1919240 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG + chr8_4:1919240-1919259 Msa1306960:CDS 35.0%
! TTTGTGAAGGGTATGATGAA+GGG + chr8_4:1919241-1919260 Msa1306960:CDS 35.0%
TACCAGTTAAAATCTTTCGG+CGG + chr8_4:1919405-1919424 Msa1306960:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG + chr8_4:1919632-1919651 Msa1306960:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG + chr8_4:1919794-1919813 Msa1306960:three_prime_UTR 35.0%
CTTCAAGAACAAGAAAGAGG+TGG - chr8_4:1918626-1918645 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG - chr8_4:1918788-1918807 None:intergenic 40.0%
! ATTTGCTACCAAGATGAGAG+TGG + chr8_4:1919210-1919229 Msa1306960:CDS 40.0%
! TACCTTTTGTGATGAACCGT+TGG - chr8_4:1919291-1919310 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG - chr8_4:1919410-1919429 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG - chr8_4:1919725-1919744 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG + chr8_4:1919725-1919744 Msa1306960:three_prime_UTR 40.0%
CTTCAAGAACAAGAAAGAGG+TGG - chr8_4:1918626-1918645 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG - chr8_4:1918788-1918807 None:intergenic 40.0%
! ATTTGCTACCAAGATGAGAG+TGG + chr8_4:1919210-1919229 Msa1306960:CDS 40.0%
! TACCTTTTGTGATGAACCGT+TGG - chr8_4:1919291-1919310 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG - chr8_4:1919410-1919429 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG - chr8_4:1919725-1919744 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG + chr8_4:1919725-1919744 Msa1306960:three_prime_UTR 40.0%
!! CTTTGGAGCATAGCATCGTT+TGG - chr8_4:1918747-1918766 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG + chr8_4:1918769-1918788 Msa1306960:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG + chr8_4:1918802-1918821 Msa1306960:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG + chr8_4:1919286-1919305 Msa1306960:CDS 45.0%
!! CTTTGGAGCATAGCATCGTT+TGG - chr8_4:1918747-1918766 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG + chr8_4:1918769-1918788 Msa1306960:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG + chr8_4:1918802-1918821 Msa1306960:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG + chr8_4:1919286-1919305 Msa1306960:CDS 45.0%
TCGGCGGTTAAATGTCGATG+AGG + chr8_4:1919421-1919440 Msa1306960:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG + chr8_4:1919646-1919665 Msa1306960:CDS 50.0%
TCGGCGGTTAAATGTCGATG+AGG + chr8_4:1919421-1919440 Msa1306960:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG + chr8_4:1919646-1919665 Msa1306960:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 1918583 1919853 1918583 ID=Msa1306960;Name=Msa1306960
chr8_4 mRNA 1918583 1919853 1918583 ID=Msa1306960-mRNA-1;Parent=Msa1306960;Name=Msa1306960-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|1|1|1|1|1|3|174|134
chr8_4 exon 1918583 1918823 1918583 ID=Msa1306960-mRNA-1:exon:642;Parent=Msa1306960-mRNA-1
chr8_4 exon 1919180 1919307 1919180 ID=Msa1306960-mRNA-1:exon:643;Parent=Msa1306960-mRNA-1
chr8_4 exon 1919571 1919853 1919571 ID=Msa1306960-mRNA-1:exon:644;Parent=Msa1306960-mRNA-1
chr8_4 five_prime_UTR 1918583 1918655 1918583 ID=Msa1306960-mRNA-1:five_prime_utr;Parent=Msa1306960-mRNA-1
chr8_4 CDS 1918656 1918823 1918656 ID=Msa1306960-mRNA-1:cds;Parent=Msa1306960-mRNA-1
chr8_4 CDS 1919180 1919307 1919180 ID=Msa1306960-mRNA-1:cds;Parent=Msa1306960-mRNA-1
chr8_4 CDS 1919571 1919679 1919571 ID=Msa1306960-mRNA-1:cds;Parent=Msa1306960-mRNA-1
chr8_4 three_prime_UTR 1919680 1919853 1919680 ID=Msa1306960-mRNA-1:three_prime_utr;Parent=Msa1306960-mRNA-1
Gene Sequence

>Msa1306960

ATGGCTGTTCACTGCAATTCTTTAGCTCAAATCCCAATTCAGAAATATGATAATTCATTTGTGAAATATTCAAACAATTTGTTGATGTCCAAACGATGCTATGCTCCAAAGAAAAAGTCAACAGTTGGTCCAGTGGTATTGAAAATCACAGCATCCATCAAGAACAAGGTTTTTTATGAGGATGAGTCTCAAGGTATAATTTGCTACCAAGATGAGAGTGGAGAAATAATTTGTGAAGGGTATGATGAAGGGCCATCTTATAGACAAATTTCAAGACCAACGGTTCATCACAAAAGGGATGTAGAAATTGTGAATCTTCTAAAACAAAGTTGGTTTCAAATTGCAAAAGGGAAAGAAATTGATGATGCAATTGAAGGTGGTCGTTTGCAAGAGGATTTAATTTGA

Protein sequence

>Msa1306960

MAVHCNSLAQIPIQKYDNSFVKYSNNLLMSKRCYAPKKKSTVGPVVLKITASIKNKVFYEDESQGIICYQDESGEIICEGYDEGPSYRQISRPTVHHKRDVEIVNLLKQSWFQIAKGKEIDDAIEGGRLQEDLI*