AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0569370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0569370 MtrunA17_Chr8g0384731 92.431 436 8 2 1 412 1 435 0.0 817
Msa0569370 MtrunA17_Chr3g0130741 67.290 428 100 4 24 412 19 445 0.0 576
Msa0569370 MtrunA17_Chr1g0159791 51.706 381 153 6 54 406 37 414 2.89e-129 379
Msa0569370 MtrunA17_Chr4g0010951 45.606 421 166 9 47 407 34 451 6.31e-116 345
Msa0569370 MtrunA17_Chr6g0484731 43.421 380 173 10 59 401 176 550 7.10e-90 282
Msa0569370 MtrunA17_Chr3g0078321 37.306 386 195 14 59 408 45 419 1.39e-75 241
Msa0569370 MtrunA17_Chr4g0010941 47.881 236 92 3 199 405 2 235 6.28e-72 225
Msa0569370 MtrunA17_Chr7g0221681 27.230 426 231 15 39 409 40 441 4.04e-29 118
Msa0569370 MtrunA17_Chr7g0249451 25.551 454 261 17 11 409 7 438 8.81e-28 114
Msa0569370 MtrunA17_Chr1g0179751 22.749 422 241 15 52 409 82 482 6.51e-27 112
Msa0569370 MtrunA17_Chr2g0320621 25.316 395 220 16 63 402 64 438 1.84e-26 110
Msa0569370 MtrunA17_Chr4g0053721 27.174 368 219 14 73 404 79 433 2.13e-26 111
Msa0569370 MtrunA17_Chr5g0401601 26.579 380 222 15 65 404 79 441 1.28e-25 109
Msa0569370 MtrunA17_Chr4g0027681 24.619 394 223 16 63 402 64 437 9.17e-25 105
Msa0569370 MtrunA17_Chr7g0265331 23.227 409 232 15 52 402 57 441 2.45e-24 104
Msa0569370 MtrunA17_Chr5g0406001 27.487 382 208 16 74 408 114 473 3.75e-24 104
Msa0569370 MtrunA17_Chr1g0189381 25.248 404 231 16 59 409 52 437 1.40e-22 99.4
Msa0569370 MtrunA17_Chr8g0371371 26.077 418 244 17 37 411 78 473 3.46e-22 98.6
Msa0569370 MtrunA17_Chr8g0352671 24.672 381 211 13 72 404 87 439 1.30e-21 96.3
Msa0569370 MtrunA17_Chr8g0386441 25.127 394 226 15 54 404 65 432 1.72e-21 95.9
Msa0569370 MtrunA17_Chr5g0425141 24.548 387 231 14 66 411 94 460 5.75e-21 95.1
Msa0569370 MtrunA17_Chr2g0333991 23.592 373 217 14 72 400 86 434 7.56e-20 90.9
Msa0569370 MtrunA17_Chr2g0311451 24.332 374 215 14 72 401 86 435 8.04e-20 90.9
Msa0569370 MtrunA17_Chr3g0124421 24.468 376 208 14 79 404 104 453 8.77e-19 88.2
Msa0569370 MtrunA17_Chr8g0371351 24.879 414 237 16 37 404 67 452 2.17e-18 87.0
Msa0569370 MtrunA17_Chr3g0130931 26.823 384 194 22 73 404 103 451 7.36e-18 85.1
Msa0569370 MtrunA17_Chr2g0307991 25.000 376 202 15 75 401 60 404 8.66e-18 84.7
Msa0569370 MtrunA17_Chr2g0310831 24.872 390 222 18 54 401 80 440 1.04e-17 84.7
Msa0569370 MtrunA17_Chr8g0352661 24.800 375 219 15 73 404 85 439 1.76e-17 84.0
Msa0569370 MtrunA17_Chr5g0419291 25.737 373 204 17 73 401 85 428 2.36e-17 83.6
Msa0569370 MtrunA17_Chr5g0395911 24.603 378 217 15 68 402 62 414 2.54e-15 77.4
Msa0569370 MtrunA17_Chr2g0308001 25.258 388 197 18 75 404 59 411 4.31e-15 76.6
Msa0569370 MtrunA17_Chr2g0310821 24.318 403 234 17 38 402 74 443 1.19e-14 75.5
Msa0569370 MtrunA17_Chr5g0442921 26.087 391 206 18 73 404 190 556 1.34e-14 75.5
Msa0569370 MtrunA17_Chr3g0099891 26.341 410 231 22 35 401 73 454 2.20e-14 74.7
Msa0569370 MtrunA17_Chr3g0110191 24.074 378 216 14 77 406 89 443 3.47e-14 73.9
Msa0569370 MtrunA17_Chr1g0179091 23.077 364 236 12 51 401 34 366 5.65e-14 72.8
Msa0569370 MtrunA17_Chr3g0087851 23.256 387 213 15 70 404 85 439 8.75e-14 72.8
Msa0569370 MtrunA17_Chr6g0484901 25.203 369 192 18 79 401 161 491 9.19e-14 72.8
Msa0569370 MtrunA17_Chr1g0165201 24.865 370 210 14 73 401 127 469 1.23e-13 72.4
Msa0569370 MtrunA17_Chr3g0099901 25.867 375 216 15 67 402 135 486 1.45e-13 72.4
Msa0569370 MtrunA17_Chr5g0434411 22.917 384 217 12 77 406 84 442 1.77e-13 71.6
Msa0569370 MtrunA17_Chr5g0415951 24.672 381 221 18 61 401 80 434 3.32e-13 70.9
Msa0569370 MtrunA17_Chr1g0212321 24.202 376 204 15 73 401 145 486 7.85e-13 70.1
Msa0569370 MtrunA17_Chr2g0291201 24.211 380 210 17 73 402 101 452 8.28e-13 69.7
Msa0569370 MtrunA17_Chr4g0023351 32.203 177 86 8 60 209 47 216 1.24e-12 68.2
Msa0569370 MtrunA17_Chr6g0486981 22.427 379 217 15 75 404 89 439 1.31e-12 68.9
Msa0569370 MtrunA17_Chr5g0395931 23.797 374 226 14 68 402 83 436 1.38e-12 68.9
Msa0569370 MtrunA17_Chr3g0095891 24.130 431 246 20 32 404 54 461 2.44e-12 68.2
Msa0569370 MtrunA17_Chr7g0275731 25.240 416 234 19 32 402 102 485 5.34e-12 67.4
Msa0569370 MtrunA17_Chr8g0390761 28.571 168 77 6 77 216 119 271 2.65e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0569370 AT4G33490.2 58.333 420 136 7 11 404 8 414 9.48e-168 477
Msa0569370 AT4G33490.1 58.418 392 124 7 11 376 5 383 1.13e-152 438
Msa0569370 AT1G44130.1 50.394 381 157 8 55 407 29 405 1.09e-131 385
Msa0569370 AT1G77480.2 47.558 389 176 5 47 408 39 426 2.10e-125 369
Msa0569370 AT1G77480.1 47.409 386 175 5 47 405 39 423 1.05e-123 366
Msa0569370 AT1G49050.1 42.636 387 173 13 59 401 187 568 2.47e-86 274
Msa0569370 AT1G49050.2 41.892 370 170 12 74 401 29 395 1.52e-80 254
Msa0569370 AT1G49050.3 40.659 273 121 9 59 292 187 457 1.82e-52 182
Msa0569370 AT1G65240.1 26.750 400 223 14 59 409 56 434 4.00e-33 130
Msa0569370 AT5G43100.2 28.307 378 210 15 65 404 66 420 1.90e-32 128
Msa0569370 AT2G36670.2 26.065 399 224 13 56 402 79 458 4.77e-32 127
Msa0569370 AT5G43100.1 28.307 378 210 15 65 404 66 420 8.42e-32 127
Msa0569370 AT3G02740.1 27.136 398 226 12 59 409 67 447 5.38e-31 124
Msa0569370 AT5G22850.1 25.735 408 234 13 48 403 52 442 5.34e-30 121
Msa0569370 AT2G36670.1 25.743 404 224 14 56 402 79 463 5.63e-30 121
Msa0569370 AT1G05840.1 26.410 390 213 12 73 409 78 446 6.75e-30 121
Msa0569370 AT3G02740.2 26.684 386 221 11 69 409 4 372 2.96e-29 118
Msa0569370 AT2G36670.4 25.587 383 208 12 74 402 33 392 3.22e-29 118
Msa0569370 AT2G36670.3 25.587 383 208 12 74 402 33 392 3.22e-29 118
Msa0569370 AT5G36260.1 25.182 413 246 11 46 411 47 443 1.97e-28 117
Msa0569370 AT1G08210.1 25.432 405 226 13 52 402 59 441 5.58e-28 115
Msa0569370 AT1G08210.3 25.432 405 226 13 52 402 107 489 6.48e-28 115
Msa0569370 AT1G08210.4 25.432 405 226 13 52 402 93 475 6.52e-28 115
Msa0569370 AT3G50050.1 27.224 371 225 14 65 404 83 439 2.28e-25 108
Msa0569370 AT1G08210.2 25.231 432 220 17 52 402 59 468 1.14e-23 103
Msa0569370 AT3G25700.1 25.455 385 214 16 73 401 82 449 1.09e-21 97.1
Msa0569370 AT2G17760.1 24.752 404 242 15 37 401 69 449 2.07e-21 96.7
Msa0569370 AT5G10080.1 25.946 370 209 12 79 402 104 454 2.46e-21 96.7
Msa0569370 AT3G51330.3 26.260 377 212 17 72 402 98 454 3.60e-20 92.4
Msa0569370 AT3G51350.1 27.200 375 203 17 74 401 101 452 3.81e-20 92.8
Msa0569370 AT4G35880.1 27.105 380 214 17 69 407 100 457 3.91e-20 92.8
Msa0569370 AT3G51330.1 26.330 376 211 17 72 401 98 453 5.42e-20 92.4
Msa0569370 AT2G28010.1 26.371 383 227 18 47 404 42 394 6.34e-20 91.3
Msa0569370 AT2G28030.1 23.467 375 236 15 54 404 43 390 1.03e-17 84.7
Msa0569370 AT2G28040.2 25.000 380 226 16 57 404 32 384 1.30e-16 81.3
Msa0569370 AT2G28040.1 25.000 380 226 16 57 404 41 393 1.84e-16 80.9
Msa0569370 AT3G51340.1 26.121 379 213 16 72 401 99 459 1.86e-16 81.6
Msa0569370 AT3G51340.2 25.606 371 213 14 72 401 99 447 7.21e-16 79.7
Msa0569370 AT2G03200.1 23.798 416 222 20 45 404 85 461 1.90e-15 78.2
Msa0569370 AT1G66180.1 22.572 381 217 14 77 404 74 429 3.34e-15 77.4
Msa0569370 AT1G01300.1 27.005 374 199 17 73 401 140 484 2.02e-14 75.5
Msa0569370 AT4G35880.2 26.648 349 193 17 69 376 100 426 2.97e-14 74.7
Msa0569370 AT3G51330.2 25.641 351 195 17 72 376 98 428 9.71e-14 73.2
Msa0569370 AT3G51360.7 22.164 379 219 16 72 402 2 352 2.92e-13 71.2
Msa0569370 AT3G51360.9 22.951 366 226 14 74 402 100 446 3.74e-13 71.2
Msa0569370 AT3G51360.1 22.951 366 226 14 74 402 90 436 5.18e-13 70.9
Msa0569370 AT3G18490.1 23.925 372 209 19 73 401 160 500 1.31e-12 69.7
Msa0569370 AT3G20015.1 24.865 370 209 16 73 401 129 470 2.51e-12 68.9
Msa0569370 AT2G42980.1 24.742 388 218 14 73 404 158 527 5.32e-12 67.8
Msa0569370 AT3G59080.1 23.389 419 243 15 44 404 137 535 1.08e-11 67.0
Msa0569370 AT3G51340.4 26.523 279 157 11 79 316 107 378 1.90e-11 65.9
Msa0569370 AT3G51340.3 26.820 261 148 9 79 300 107 363 1.93e-11 65.9

Find 147 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTGTGTTCCCTATTTA+TGG 0.060827 4_1:+71223591 Msa0569370:CDS
AAACTGAAATCTCTGGATTT+TGG 0.144007 4_1:-71227138 None:intergenic
AGATAGGTAGGGTGGGATTA+AGG 0.217206 4_1:+71223418 Msa0569370:CDS
GGATAAACGTTGCCATAAAT+AGG 0.257987 4_1:-71223603 None:intergenic
AACGAATCGATTGATGAAAT+TGG 0.264253 4_1:-71225554 None:intergenic
TTTCCAAATGCAGCTGAATA+AGG 0.273485 4_1:+71226393 Msa0569370:intron
ATTTCATAGTCACATTGATT+AGG 0.289901 4_1:-71224738 None:intergenic
CACCCTTTAGATGGAATACT+TGG 0.291237 4_1:+71225386 Msa0569370:CDS
TTACCAAGCAATGATTTCTT+TGG 0.294065 4_1:+71225797 Msa0569370:CDS
AGTGTCAACATCAAGAAAAT+AGG 0.297686 4_1:-71224268 None:intergenic
TTTCATAGTCACATTGATTA+GGG 0.312831 4_1:-71224737 None:intergenic
AGCATTGTGTAGAGTATATT+GGG 0.314014 4_1:+71227314 Msa0569370:three_prime_UTR
TCAACTTACCACCCTTTAGA+TGG 0.325308 4_1:+71225377 Msa0569370:CDS
CTAAATTCTCACCTCCAATT+AGG 0.327781 4_1:-71226774 None:intergenic
AAGCATTGTGTAGAGTATAT+TGG 0.329838 4_1:+71227313 Msa0569370:three_prime_UTR
TTGGGTGAACTGAACCTAAT+TGG 0.334057 4_1:+71226760 Msa0569370:CDS
TCTTTAAAAGTACCTAATTA+AGG 0.339236 4_1:+71227165 Msa0569370:three_prime_UTR
TTTAAATTATTGATTCTTGT+TGG 0.351690 4_1:-71223258 None:intergenic
GTCATATGCATGCCTTAATT+AGG 0.358204 4_1:-71227177 None:intergenic
GATAGGTAGGGTGGGATTAA+GGG 0.398450 4_1:+71223419 Msa0569370:CDS
AGATCATAAACTGAAATCTC+TGG 0.399104 4_1:-71227145 None:intergenic
AGTTCACCCAATCCTACTTC+AGG 0.414059 4_1:-71226748 None:intergenic
GGAGCAGGAAAGAAGTTTCC+AGG 0.414558 4_1:+71222937 None:intergenic
AAGTTGAAGGTGAAAATATC+TGG 0.417456 4_1:-71225361 None:intergenic
GACAACGAGAAGCAGTTGAT+TGG 0.418942 4_1:+71227087 Msa0569370:CDS
GTGCCATTCTAACTTTAAGT+TGG 0.419171 4_1:-71224836 None:intergenic
AGAAGAAGATGAAGATAGGT+AGG 0.420924 4_1:+71223406 Msa0569370:exon
ATGGCAACGTTTATCCTGTT+GGG 0.427167 4_1:+71223610 Msa0569370:CDS
GATAAACGTTGCCATAAATA+GGG 0.428708 4_1:-71223602 None:intergenic
GTGTCAACATCAAGAAAATA+GGG 0.431013 4_1:-71224267 None:intergenic
ATAGGTAAAGTCTGGTCATC+AGG 0.433183 4_1:-71226445 None:intergenic
AACAAGAAGAAGATGAAGAT+AGG 0.439947 4_1:+71223402 Msa0569370:exon
TTAAATATTATCTTTGACAC+TGG 0.441413 4_1:+71225744 Msa0569370:CDS
ACTTGTCTTCTTGTTCAGCT+TGG 0.441843 4_1:+71223475 Msa0569370:CDS
GAAGATGAAGATAGGTAGGG+TGG 0.442669 4_1:+71223410 Msa0569370:exon
CTGAGAGTTGAAGTATGTAT+AGG 0.443665 4_1:-71225772 None:intergenic
GATATTATAAGATAAGCTTC+AGG 0.447328 4_1:-71226583 None:intergenic
TGAAAGAAATTGAAGGATAA+TGG 0.452938 4_1:-71223299 None:intergenic
GATATCACAGTTGAATAGTC+AGG 0.453876 4_1:+71225433 Msa0569370:CDS
AAGTTGAAAAGTTGTAGAAA+AGG 0.460593 4_1:-71223069 None:intergenic
TTGGTGAAACTAAGTGTTAG+TGG 0.461386 4_1:-71226526 None:intergenic
AGTCCAAGTATTCCATCTAA+AGG 0.461510 4_1:-71225389 None:intergenic
GGTGGGATTAAGGGTGATGA+TGG 0.463114 4_1:+71223428 Msa0569370:CDS
AACTTTAAGTTGGACTCCAT+TGG 0.463263 4_1:-71224826 None:intergenic
TATGGCAACGTTTATCCTGT+TGG 0.466319 4_1:+71223609 Msa0569370:CDS
GTTATTTGTTGCAAGAATAT+GGG 0.467337 4_1:+71226697 Msa0569370:intron
TCCTCTATGATCTATAGTAA+TGG 0.469339 4_1:-71223377 None:intergenic
TTAGATGGAATACTTGGACT+TGG 0.471121 4_1:+71225392 Msa0569370:CDS
TGTACTAGTTCATGAAGAAA+AGG 0.472388 4_1:-71223224 None:intergenic
GAACGGCCCTGAAGTAGGAT+TGG 0.472405 4_1:+71226741 Msa0569370:CDS
TGATTGATTAGATTATTTCC+TGG 0.472792 4_1:-71222955 None:intergenic
GGAATACTATTGCAATCTGC+AGG 0.473909 4_1:-71227117 None:intergenic
GTCCAAGTATTCCATCTAAA+GGG 0.477058 4_1:-71225388 None:intergenic
ATTAATGCATATTATAGAGC+AGG 0.483123 4_1:+71227226 Msa0569370:three_prime_UTR
ATCGTATCACGTTAATTTGA+CGG 0.486448 4_1:+71227401 Msa0569370:three_prime_UTR
ACCTCAGAACATTGGGTACA+AGG 0.488228 4_1:-71224324 None:intergenic
ACATCAAGAAAATAGGGCCT+TGG 0.492843 4_1:-71224261 None:intergenic
AATGAAATATCATACCCAAC+AGG 0.493100 4_1:-71223624 None:intergenic
GGAGGAAGGAAGACCGGGGT+TGG 0.493414 4_1:+71225717 Msa0569370:CDS
TAATGTTACCTCAGAACATT+GGG 0.497109 4_1:-71224331 None:intergenic
CTTAAAGTTAGAATGGCACT+TGG 0.502205 4_1:+71224840 Msa0569370:CDS
GCCAGCACATAGGTAAAGTC+TGG 0.502564 4_1:-71226453 None:intergenic
AAGATGAAGATAGGTAGGGT+GGG 0.502567 4_1:+71223411 Msa0569370:exon
AACGGCCCTGAAGTAGGATT+GGG 0.503963 4_1:+71226742 Msa0569370:CDS
GTTCACCCAATCCTACTTCA+GGG 0.504181 4_1:-71226747 None:intergenic
ATGATTCTTCTCGAATGAGT+TGG 0.504399 4_1:+71225528 Msa0569370:CDS
TACCATCACTTTGTCTAGCA+TGG 0.505530 4_1:-71227061 None:intergenic
ACAGTTGAATAGTCAGGGCT+TGG 0.506595 4_1:+71225439 Msa0569370:CDS
ATTGGGGTAAGCTATTATGC+AGG 0.506850 4_1:+71227331 Msa0569370:three_prime_UTR
AGGTTTAACTCTTCCACCAT+TGG 0.508559 4_1:-71226545 None:intergenic
AGTCCAACTTAAAGTTAGAA+TGG 0.510476 4_1:+71224833 Msa0569370:CDS
TCAAGAAAATAGGGCCTTGG+TGG 0.511612 4_1:-71224258 None:intergenic
TACCTTCTCAACTTCACCAA+TGG 0.512014 4_1:+71224810 Msa0569370:CDS
CATACCTTGTGAGTAAGAAA+AGG 0.514476 4_1:+71223140 Msa0569370:five_prime_UTR
ACGAGAAGCAGTTGATTGGA+TGG 0.515480 4_1:+71227091 Msa0569370:CDS
CTATACATACTTCAACTCTC+AGG 0.516576 4_1:+71225773 Msa0569370:CDS
TGTTTAAGCTCGCGAGGAGG+GGG 0.519451 4_1:+71225482 Msa0569370:CDS
TGGGAGAGCGTGTGAATGAA+TGG 0.519940 4_1:-71223006 None:intergenic
ATGGAATACTTGGACTTGGA+AGG 0.520338 4_1:+71225396 Msa0569370:CDS
TTTGATAATAAACGCAAAAG+TGG 0.521006 4_1:+71223498 Msa0569370:CDS
ATTCTGAACGGCCCTGAAGT+AGG 0.523129 4_1:+71226736 Msa0569370:CDS
TGGTGAATGAGCAAGTGTTA+AGG 0.528403 4_1:-71222986 None:intergenic
CTTGAAGACATGGCTTCACC+TGG 0.531998 4_1:-71223537 None:intergenic
TCTAAAGGGTGGTAAGTTGA+AGG 0.532635 4_1:-71225374 None:intergenic
TGGAATACTTGGACTTGGAA+GGG 0.533058 4_1:+71225397 Msa0569370:CDS
TTCACATGTGTAGTCATCGG+TGG 0.533364 4_1:-71224712 None:intergenic
GAAAATAGGGCCTTGGTGGT+TGG 0.533650 4_1:-71224254 None:intergenic
GGGCTTGGTGCGAAATGTGA+TGG 0.536228 4_1:+71225454 Msa0569370:CDS
CGAGAAGCAGTTGATTGGAT+GGG 0.543766 4_1:+71227092 Msa0569370:CDS
AGGGGAAAACATTACTCTGC+AGG 0.544182 4_1:+71225675 Msa0569370:intron
TGATTCTTGTTGGAAAGAGT+TGG 0.546273 4_1:-71223248 None:intergenic
AATATGGGAAATGTTTGTCT+AGG 0.546632 4_1:+71226712 Msa0569370:CDS
ACCAGACTTTACCTATGTGC+TGG 0.546884 4_1:+71226452 Msa0569370:CDS
CTCCATTGGTGAAGTTGAGA+AGG 0.547745 4_1:-71224812 None:intergenic
ATAAACTCAACATTAGAAAG+TGG 0.548243 4_1:-71223195 None:intergenic
GGAATACTTGGACTTGGAAG+GGG 0.550065 4_1:+71225398 Msa0569370:CDS
AGAAATTGAAGGATAATGGT+GGG 0.551649 4_1:-71223295 None:intergenic
ACACTTAGTTTCACCAATGG+TGG 0.552466 4_1:+71226532 Msa0569370:CDS
GTAATGTTACCTCAGAACAT+TGG 0.553528 4_1:-71224332 None:intergenic
AGTCTCATGAACCATGCTGC+TGG 0.557344 4_1:+71223564 Msa0569370:CDS
TCAGGAGGGATCTCAAACTG+AGG 0.558793 4_1:-71226565 None:intergenic
ATAAATAGGGAACACAATTG+AGG 0.560664 4_1:-71223589 None:intergenic
GAAGAAGATGAAGATAGGTA+GGG 0.561089 4_1:+71223407 Msa0569370:exon
GTCTTCACATGTGTAGTCAT+CGG 0.562506 4_1:-71224715 None:intergenic
GCTGATCAATACTCAACCCT+TGG 0.562559 4_1:+71224768 Msa0569370:CDS
TGACCAAAGAAATCATTGCT+TGG 0.564667 4_1:-71225800 None:intergenic
AAGAAATTGAAGGATAATGG+TGG 0.566068 4_1:-71223296 None:intergenic
ACTTCATTTATGCTTCTGAA+AGG 0.566258 4_1:-71226487 None:intergenic
ATATCACAGTTGAATAGTCA+GGG 0.567767 4_1:+71225434 Msa0569370:CDS
GAGACTTGGTCTTGAAGACA+TGG 0.568180 4_1:-71223547 None:intergenic
GTATGGATGAAAGAAATTGA+AGG 0.568953 4_1:-71223306 None:intergenic
ATTGTTTAAGCTCGCGAGGA+GGG 0.569291 4_1:+71225480 Msa0569370:CDS
TCACATGTGTAGTCATCGGT+GGG 0.576365 4_1:-71224711 None:intergenic
TCCATTACTATAGATCATAG+AGG 0.586543 4_1:+71223376 Msa0569370:five_prime_UTR
TATAATGTTACTCTCAACAT+AGG 0.586798 4_1:+71224231 Msa0569370:CDS
GCAGCATGGTTCATGAGACT+TGG 0.589743 4_1:-71223561 None:intergenic
CATTGTTTAAGCTCGCGAGG+AGG 0.594028 4_1:+71225479 Msa0569370:CDS
GAGAAGCAGTTGATTGGATG+GGG 0.594642 4_1:+71227093 Msa0569370:CDS
GGCTTGGTGCGAAATGTGAT+GGG 0.595102 4_1:+71225455 Msa0569370:CDS
CATATTATAGAGCAGGGTGA+TGG 0.595503 4_1:+71227233 Msa0569370:three_prime_UTR
ATTCAACTGTGATATCAAGC+TGG 0.597014 4_1:-71225426 None:intergenic
TTAATGCATATTATAGAGCA+GGG 0.602995 4_1:+71227227 Msa0569370:three_prime_UTR
ACTTGGACTTGGAAGGGGAA+AGG 0.604303 4_1:+71225403 Msa0569370:CDS
TTGGGGTAAGCTATTATGCA+GGG 0.608982 4_1:+71227332 Msa0569370:three_prime_UTR
CTAACACTTAGTTTCACCAA+TGG 0.609380 4_1:+71226529 Msa0569370:CDS
ACTTTACCTATGTGCTGGCA+TGG 0.609706 4_1:+71226457 Msa0569370:CDS
TTGTTTAAGCTCGCGAGGAG+GGG 0.611048 4_1:+71225481 Msa0569370:CDS
ATCATTTAGAAGTACACCAA+GGG 0.613371 4_1:-71224784 None:intergenic
GGACATTGTTTAAGCTCGCG+AGG 0.614419 4_1:+71225476 Msa0569370:CDS
AATATTTAAACTTCCAACCC+CGG 0.615370 4_1:-71225730 None:intergenic
TTAAAGTTAGAATGGCACTT+GGG 0.620505 4_1:+71224841 Msa0569370:CDS
ATTATAAGATAAGCTTCAGG+AGG 0.624826 4_1:-71226580 None:intergenic
GAAATTGAAGGATAATGGTG+GGG 0.633191 4_1:-71223294 None:intergenic
TCCTTGTACCCAATGTTCTG+AGG 0.637225 4_1:+71224323 Msa0569370:CDS
ATCCATGCTAGACAAAGTGA+TGG 0.638414 4_1:+71227059 Msa0569370:CDS
ACACTGGTAGCGAACTCACA+TGG 0.642611 4_1:+71224286 Msa0569370:CDS
ATCTTTGCAAGATTCATGCG+AGG 0.643613 4_1:+71223116 Msa0569370:five_prime_UTR
GGTGAACTGAACCTAATTGG+AGG 0.647086 4_1:+71226763 Msa0569370:CDS
CAAGTATTCCATCTAAAGGG+TGG 0.648915 4_1:-71225385 None:intergenic
GGGTACAAGGAGCATCACAT+TGG 0.654731 4_1:-71224311 None:intergenic
GTTTGTCTAGGCATTCTGAA+CGG 0.654989 4_1:+71226724 Msa0569370:CDS
CATCATTTAGAAGTACACCA+AGG 0.656080 4_1:-71224785 None:intergenic
AATTTGACGGCATTAGCACG+AGG 0.660806 4_1:+71227414 Msa0569370:three_prime_UTR
CAATTGAGGAACCAGCAGCA+TGG 0.669395 4_1:-71223575 None:intergenic
GCATTGTGTAGAGTATATTG+GGG 0.685659 4_1:+71227315 Msa0569370:three_prime_UTR
TCAACATAGGCCAACCACCA+AGG 0.689202 4_1:+71224244 Msa0569370:CDS
TTATAAGATAAGCTTCAGGA+GGG 0.705622 4_1:-71226579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAATAATAATAAAT+GGG - chr4_1:71223028-71223047 None:intergenic 0.0%
!! ATAATAATAATAATAATAAA+TGG - chr4_1:71223029-71223048 None:intergenic 0.0%
!!! TTTATTTTTACTATAATGTA+TGG - chr4_1:71223326-71223345 None:intergenic 10.0%
!!! AATAATGTTTTTCTTTTTTA+AGG + chr4_1:71223695-71223714 Msa0569370:intron 10.0%
!! ATTAAAATTCAATTCATAAA+AGG - chr4_1:71223770-71223789 None:intergenic 10.0%
!!! TGAATTGAATTTTAATTTTT+TGG + chr4_1:71223774-71223793 Msa0569370:intron 10.0%
!!! AAATTGATATTCATTTTTAT+TGG + chr4_1:71224032-71224051 Msa0569370:intron 10.0%
!!! ATTTATATTATTTTCTAGAA+TGG - chr4_1:71226125-71226144 None:intergenic 10.0%
!!! ATTAATAATTTCTAGTATAA+AGG - chr4_1:71226341-71226360 None:intergenic 10.0%
!! TTATACTAGAAATTATTAAT+TGG + chr4_1:71226341-71226360 Msa0569370:intron 10.0%
!!! TTTAAATTATTGATTCTTGT+TGG - chr4_1:71223261-71223280 None:intergenic 15.0%
!! ATATTATATGCAATTTATGT+AGG - chr4_1:71224579-71224598 None:intergenic 15.0%
!! ATGATTTATATAAGTGAATT+AGG + chr4_1:71225006-71225025 Msa0569370:intron 15.0%
!! ATTAACATAAGATATAATCA+AGG + chr4_1:71226058-71226077 Msa0569370:intron 15.0%
!!! AATATTTTGTGATTTCAAAA+TGG + chr4_1:71226150-71226169 Msa0569370:intron 15.0%
!! TAAGTATTTAATGATGAAAT+TGG - chr4_1:71226277-71226296 None:intergenic 15.0%
!! TTGTTAACTGTTATAATAAA+TGG + chr4_1:71226638-71226657 Msa0569370:intron 15.0%
!! ATGATATTTCATTTCTTTCT+AGG + chr4_1:71223634-71223653 Msa0569370:intron 20.0%
!!! TTTCTTGTTTATCAATTCTT+AGG + chr4_1:71223883-71223902 Msa0569370:intron 20.0%
!!! GGTTTTCTTTTATTTAATTG+TGG - chr4_1:71223988-71224007 None:intergenic 20.0%
!!! ACAGACAAATGTATTTTTTT+GGG + chr4_1:71224055-71224074 Msa0569370:intron 20.0%
!! ATTAGAATATCAATTTCATG+AGG - chr4_1:71224176-71224195 None:intergenic 20.0%
!! TTCACTTATATAAATCATTC+TGG - chr4_1:71225004-71225023 None:intergenic 20.0%
!! AATAGATATGACATAAATTG+TGG - chr4_1:71225277-71225296 None:intergenic 20.0%
!! TTAAATATTATCTTTGACAC+TGG + chr4_1:71225744-71225763 Msa0569370:CDS 20.0%
!!! GTATTTTTAGTTAACTAGTT+TGG + chr4_1:71225992-71226011 Msa0569370:intron 20.0%
!!! TTGTTTATTTATTTCTGTCA+TGG - chr4_1:71226307-71226326 None:intergenic 20.0%
!! ATATTATGTTATGTAAGTGT+TGG + chr4_1:71227015-71227034 Msa0569370:intron 20.0%
!!! TCTTTAAAAGTACCTAATTA+AGG + chr4_1:71227165-71227184 Msa0569370:three_prime_UTR 20.0%
!!! GTTTTATTACTTTATCTTCA+TGG + chr4_1:71227265-71227284 Msa0569370:three_prime_UTR 20.0%
! TGATTGATTAGATTATTTCC+TGG - chr4_1:71222958-71222977 None:intergenic 25.0%
! AAGTTGAAAAGTTGTAGAAA+AGG - chr4_1:71223072-71223091 None:intergenic 25.0%
! ATAAACTCAACATTAGAAAG+TGG - chr4_1:71223198-71223217 None:intergenic 25.0%
! TGAAAGAAATTGAAGGATAA+TGG - chr4_1:71223302-71223321 None:intergenic 25.0%
!! TTTGATAATAAACGCAAAAG+TGG + chr4_1:71223498-71223517 Msa0569370:CDS 25.0%
!!! GACAGACAAATGTATTTTTT+TGG + chr4_1:71224054-71224073 Msa0569370:intron 25.0%
!!! TGTATTTTTTTGGGACATTT+TGG + chr4_1:71224064-71224083 Msa0569370:intron 25.0%
! TATAATGTTACTCTCAACAT+AGG + chr4_1:71224231-71224250 Msa0569370:CDS 25.0%
!!! ATGTGCTGATTATAATTGAT+TGG + chr4_1:71224504-71224523 Msa0569370:intron 25.0%
! CAGCAAAAAAAAAATTACTC+TGG + chr4_1:71224534-71224553 Msa0569370:intron 25.0%
! CAAAAAAAAAATTACTCTGG+TGG + chr4_1:71224537-71224556 Msa0569370:intron 25.0%
!! TTTCATAGTCACATTGATTA+GGG - chr4_1:71224740-71224759 None:intergenic 25.0%
!! ATTTCATAGTCACATTGATT+AGG - chr4_1:71224741-71224760 None:intergenic 25.0%
! CATGTCATTATATTGTTGAA+AGG - chr4_1:71225235-71225254 None:intergenic 25.0%
!! TGATTACATTACTTCTAAGA+GGG - chr4_1:71225850-71225869 None:intergenic 25.0%
! TTGATTACATTACTTCTAAG+AGG - chr4_1:71225851-71225870 None:intergenic 25.0%
! GTAATGTAATCAAGTAGTTA+TGG + chr4_1:71225858-71225877 Msa0569370:intron 25.0%
! TCAAGTAGTTATGGTTATTA+TGG + chr4_1:71225867-71225886 Msa0569370:intron 25.0%
! TTTCAATTTCAGTATATCCA+AGG + chr4_1:71226089-71226108 Msa0569370:intron 25.0%
! TTTATGTGTATTACACAACA+GGG - chr4_1:71226192-71226211 None:intergenic 25.0%
! ATTTATGTGTATTACACAAC+AGG - chr4_1:71226193-71226212 None:intergenic 25.0%
! ACTAGAAATTATTAATTGGC+AGG + chr4_1:71226345-71226364 Msa0569370:intron 25.0%
! GCATTTGGAAAATTAACAAA+AGG - chr4_1:71226384-71226403 None:intergenic 25.0%
! GATATTATAAGATAAGCTTC+AGG - chr4_1:71226586-71226605 None:intergenic 25.0%
!! TGTTATTTGTTGCAAGAATA+TGG + chr4_1:71226696-71226715 Msa0569370:intron 25.0%
!! GTTATTTGTTGCAAGAATAT+GGG + chr4_1:71226697-71226716 Msa0569370:intron 25.0%
! ATTAATGCATATTATAGAGC+AGG + chr4_1:71227226-71227245 Msa0569370:three_prime_UTR 25.0%
! TTAATGCATATTATAGAGCA+GGG + chr4_1:71227227-71227246 Msa0569370:three_prime_UTR 25.0%
! TTATCCTTTTCTTACTCACA+AGG - chr4_1:71223147-71223166 None:intergenic 30.0%
TGTACTAGTTCATGAAGAAA+AGG - chr4_1:71223227-71223246 None:intergenic 30.0%
! AGAAATTGAAGGATAATGGT+GGG - chr4_1:71223298-71223317 None:intergenic 30.0%
! AAGAAATTGAAGGATAATGG+TGG - chr4_1:71223299-71223318 None:intergenic 30.0%
GTATGGATGAAAGAAATTGA+AGG - chr4_1:71223309-71223328 None:intergenic 30.0%
TCCATTACTATAGATCATAG+AGG + chr4_1:71223376-71223395 Msa0569370:five_prime_UTR 30.0%
TCCTCTATGATCTATAGTAA+TGG - chr4_1:71223380-71223399 None:intergenic 30.0%
AACAAGAAGAAGATGAAGAT+AGG + chr4_1:71223402-71223421 Msa0569370:exon 30.0%
ATAAATAGGGAACACAATTG+AGG - chr4_1:71223592-71223611 None:intergenic 30.0%
TCAATTGTGTTCCCTATTTA+TGG + chr4_1:71223591-71223610 Msa0569370:CDS 30.0%
GATAAACGTTGCCATAAATA+GGG - chr4_1:71223605-71223624 None:intergenic 30.0%
AATGAAATATCATACCCAAC+AGG - chr4_1:71223627-71223646 None:intergenic 30.0%
AATTTCCAAGCAACAGAAAA+GGG - chr4_1:71223909-71223928 None:intergenic 30.0%
AAATTTCCAAGCAACAGAAA+AGG - chr4_1:71223910-71223929 None:intergenic 30.0%
AGTTTAAGGGGTTAAAAGAT+CGG - chr4_1:71224132-71224151 None:intergenic 30.0%
CTGCAAAAGTTGAAATTTAC+GGG - chr4_1:71224207-71224226 None:intergenic 30.0%
CCTGCAAAAGTTGAAATTTA+CGG - chr4_1:71224208-71224227 None:intergenic 30.0%
GTGTCAACATCAAGAAAATA+GGG - chr4_1:71224270-71224289 None:intergenic 30.0%
AGTGTCAACATCAAGAAAAT+AGG - chr4_1:71224271-71224290 None:intergenic 30.0%
! TATTTTCTTGATGTTGACAC+TGG + chr4_1:71224270-71224289 Msa0569370:CDS 30.0%
TAATGTTACCTCAGAACATT+GGG - chr4_1:71224334-71224353 None:intergenic 30.0%
! ATCATTTAGAAGTACACCAA+GGG - chr4_1:71224787-71224806 None:intergenic 30.0%
AGTCCAACTTAAAGTTAGAA+TGG + chr4_1:71224833-71224852 Msa0569370:CDS 30.0%
TTAAAGTTAGAATGGCACTT+GGG + chr4_1:71224841-71224860 Msa0569370:CDS 30.0%
ATATTTCAAGAGAACATGCA+AGG - chr4_1:71224879-71224898 None:intergenic 30.0%
TTTGTGGAATGGTAAAACTT+AGG - chr4_1:71225166-71225185 None:intergenic 30.0%
TCAAATAGTAGATCATGCAT+TGG - chr4_1:71225191-71225210 None:intergenic 30.0%
TTGATTCCAATATACAGATG+TGG + chr4_1:71225332-71225351 Msa0569370:intron 30.0%
TCATATCCACATCTGTATAT+TGG - chr4_1:71225341-71225360 None:intergenic 30.0%
AAGTTGAAGGTGAAAATATC+TGG - chr4_1:71225364-71225383 None:intergenic 30.0%
ATATCACAGTTGAATAGTCA+GGG + chr4_1:71225434-71225453 Msa0569370:CDS 30.0%
AACGAATCGATTGATGAAAT+TGG - chr4_1:71225557-71225576 None:intergenic 30.0%
!! TCTTTTATGTTGTTTGTGTC+AGG + chr4_1:71225655-71225674 Msa0569370:intron 30.0%
!! CTTTTATGTTGTTTGTGTCA+GGG + chr4_1:71225656-71225675 Msa0569370:intron 30.0%
!! TTTTATGTTGTTTGTGTCAG+GGG + chr4_1:71225657-71225676 Msa0569370:intron 30.0%
AATATTTAAACTTCCAACCC+CGG - chr4_1:71225733-71225752 None:intergenic 30.0%
TTACCAAGCAATGATTTCTT+TGG + chr4_1:71225797-71225816 Msa0569370:CDS 30.0%
! ATTACATTACTTCTAAGAGG+GGG - chr4_1:71225848-71225867 None:intergenic 30.0%
! GATTACATTACTTCTAAGAG+GGG - chr4_1:71225849-71225868 None:intergenic 30.0%
ACTTCATTTATGCTTCTGAA+AGG - chr4_1:71226490-71226509 None:intergenic 30.0%
TTATAAGATAAGCTTCAGGA+GGG - chr4_1:71226582-71226601 None:intergenic 30.0%
ATTATAAGATAAGCTTCAGG+AGG - chr4_1:71226583-71226602 None:intergenic 30.0%
AATATGGGAAATGTTTGTCT+AGG + chr4_1:71226712-71226731 Msa0569370:CDS 30.0%
! TTAGCTACATTGTCGTATTT+TGG + chr4_1:71226793-71226812 Msa0569370:intron 30.0%
! AAACTGAAATCTCTGGATTT+TGG - chr4_1:71227141-71227160 None:intergenic 30.0%
AGATCATAAACTGAAATCTC+TGG - chr4_1:71227148-71227167 None:intergenic 30.0%
! AAGCATTGTGTAGAGTATAT+TGG + chr4_1:71227313-71227332 Msa0569370:three_prime_UTR 30.0%
AGCATTGTGTAGAGTATATT+GGG + chr4_1:71227314-71227333 Msa0569370:three_prime_UTR 30.0%
! ATCGTATCACGTTAATTTGA+CGG + chr4_1:71227401-71227420 Msa0569370:three_prime_UTR 30.0%
CATACCTTGTGAGTAAGAAA+AGG + chr4_1:71223140-71223159 Msa0569370:five_prime_UTR 35.0%
TGATTCTTGTTGGAAAGAGT+TGG - chr4_1:71223251-71223270 None:intergenic 35.0%
! GAAATTGAAGGATAATGGTG+GGG - chr4_1:71223297-71223316 None:intergenic 35.0%
AGAAGAAGATGAAGATAGGT+AGG + chr4_1:71223406-71223425 Msa0569370:exon 35.0%
GAAGAAGATGAAGATAGGTA+GGG + chr4_1:71223407-71223426 Msa0569370:exon 35.0%
! GGAAGAAACTCAATTTTACC+AGG + chr4_1:71223519-71223538 Msa0569370:CDS 35.0%
GGATAAACGTTGCCATAAAT+AGG - chr4_1:71223606-71223625 None:intergenic 35.0%
TGAGTATCCAAATTCATACC+AGG + chr4_1:71223847-71223866 Msa0569370:intron 35.0%
AGAAAGTAATATTCCAGTCC+TGG - chr4_1:71223868-71223887 None:intergenic 35.0%
AAAACCTCTTCTGACATTGA+TGG + chr4_1:71224002-71224021 Msa0569370:intron 35.0%
AAACCTCTTCTGACATTGAT+GGG + chr4_1:71224003-71224022 Msa0569370:intron 35.0%
TTAACCCCTTAAACTCAAAC+CGG + chr4_1:71224137-71224156 Msa0569370:intron 35.0%
!! TTCAACCGGTTTGAGTTTAA+GGG - chr4_1:71224145-71224164 None:intergenic 35.0%
CAAAAGTTGAAATTTACGGG+CGG - chr4_1:71224204-71224223 None:intergenic 35.0%
! CCGTAAATTTCAACTTTTGC+AGG + chr4_1:71224205-71224224 Msa0569370:intron 35.0%
GTAATGTTACCTCAGAACAT+TGG - chr4_1:71224335-71224354 None:intergenic 35.0%
ATGTATCTATAGGCAAGGAT+GGG - chr4_1:71224430-71224449 None:intergenic 35.0%
TATGTATCTATAGGCAAGGA+TGG - chr4_1:71224431-71224450 None:intergenic 35.0%
TCAACGCGTTATGTATCTAT+AGG - chr4_1:71224440-71224459 None:intergenic 35.0%
CAGGGTATAAAATCATTGCT+AGG - chr4_1:71224663-71224682 None:intergenic 35.0%
! CATCATTTAGAAGTACACCA+AGG - chr4_1:71224788-71224807 None:intergenic 35.0%
!! AACTTTAAGTTGGACTCCAT+TGG - chr4_1:71224829-71224848 None:intergenic 35.0%
! GTGCCATTCTAACTTTAAGT+TGG - chr4_1:71224839-71224858 None:intergenic 35.0%
CTTAAAGTTAGAATGGCACT+TGG + chr4_1:71224840-71224859 Msa0569370:CDS 35.0%
GTCCAAGTATTCCATCTAAA+GGG - chr4_1:71225391-71225410 None:intergenic 35.0%
AGTCCAAGTATTCCATCTAA+AGG - chr4_1:71225392-71225411 None:intergenic 35.0%
TTAGATGGAATACTTGGACT+TGG + chr4_1:71225392-71225411 Msa0569370:CDS 35.0%
ATTCAACTGTGATATCAAGC+TGG - chr4_1:71225429-71225448 None:intergenic 35.0%
GATATCACAGTTGAATAGTC+AGG + chr4_1:71225433-71225452 Msa0569370:CDS 35.0%
ATGATTCTTCTCGAATGAGT+TGG + chr4_1:71225528-71225547 Msa0569370:CDS 35.0%
CTGAGAGTTGAAGTATGTAT+AGG - chr4_1:71225775-71225794 None:intergenic 35.0%
CTATACATACTTCAACTCTC+AGG + chr4_1:71225773-71225792 Msa0569370:CDS 35.0%
TGACCAAAGAAATCATTGCT+TGG - chr4_1:71225803-71225822 None:intergenic 35.0%
GTGTTTCTCGTTAGTTAGTT+TGG + chr4_1:71225961-71225980 Msa0569370:intron 35.0%
TTTCCAAATGCAGCTGAATA+AGG + chr4_1:71226393-71226412 Msa0569370:intron 35.0%
ATTCCTTATTCAGCTGCATT+TGG - chr4_1:71226399-71226418 None:intergenic 35.0%
TTGGTGAAACTAAGTGTTAG+TGG - chr4_1:71226529-71226548 None:intergenic 35.0%
CTAACACTTAGTTTCACCAA+TGG + chr4_1:71226529-71226548 Msa0569370:CDS 35.0%
CGTAGAAGAGTTTGATAAGT+TGG - chr4_1:71226617-71226636 None:intergenic 35.0%
CTAAATTCTCACCTCCAATT+AGG - chr4_1:71226777-71226796 None:intergenic 35.0%
!! TGGTGCTACATAGATAGTAA+TGG + chr4_1:71226939-71226958 Msa0569370:intron 35.0%
GTCATATGCATGCCTTAATT+AGG - chr4_1:71227180-71227199 None:intergenic 35.0%
GCATTGTGTAGAGTATATTG+GGG + chr4_1:71227315-71227334 Msa0569370:three_prime_UTR 35.0%
TGGTGAATGAGCAAGTGTTA+AGG - chr4_1:71222989-71223008 None:intergenic 40.0%
ATCTTTGCAAGATTCATGCG+AGG + chr4_1:71223116-71223135 Msa0569370:five_prime_UTR 40.0%
AAGATGAAGATAGGTAGGGT+GGG + chr4_1:71223411-71223430 Msa0569370:exon 40.0%
ACTTGTCTTCTTGTTCAGCT+TGG + chr4_1:71223475-71223494 Msa0569370:CDS 40.0%
TATGGCAACGTTTATCCTGT+TGG + chr4_1:71223609-71223628 Msa0569370:CDS 40.0%
ATGGCAACGTTTATCCTGTT+GGG + chr4_1:71223610-71223629 Msa0569370:CDS 40.0%
ATCCAAATTCATACCAGGAC+TGG + chr4_1:71223852-71223871 Msa0569370:intron 40.0%
TTCCAGTCCTGGTATGAATT+TGG - chr4_1:71223857-71223876 None:intergenic 40.0%
AACCTCTTCTGACATTGATG+GGG + chr4_1:71224004-71224023 Msa0569370:intron 40.0%
!! TCAACCGGTTTGAGTTTAAG+GGG - chr4_1:71224144-71224163 None:intergenic 40.0%
!! CTTCAACCGGTTTGAGTTTA+AGG - chr4_1:71224146-71224165 None:intergenic 40.0%
ACATCAAGAAAATAGGGCCT+TGG - chr4_1:71224264-71224283 None:intergenic 40.0%
GCGTTATGTATCTATAGGCA+AGG - chr4_1:71224435-71224454 None:intergenic 40.0%
GTCTTCACATGTGTAGTCAT+CGG - chr4_1:71224718-71224737 None:intergenic 40.0%
TACCTTCTCAACTTCACCAA+TGG + chr4_1:71224810-71224829 Msa0569370:CDS 40.0%
TGCTATTGTCTCTATGTGTG+AGG - chr4_1:71225138-71225157 None:intergenic 40.0%
GACATAAATTGTGGTCATCG+TGG - chr4_1:71225268-71225287 None:intergenic 40.0%
! TCTAAAGGGTGGTAAGTTGA+AGG - chr4_1:71225377-71225396 None:intergenic 40.0%
TCAACTTACCACCCTTTAGA+TGG + chr4_1:71225377-71225396 Msa0569370:CDS 40.0%
CAAGTATTCCATCTAAAGGG+TGG - chr4_1:71225388-71225407 None:intergenic 40.0%
CACCCTTTAGATGGAATACT+TGG + chr4_1:71225386-71225405 Msa0569370:CDS 40.0%
ATGGAATACTTGGACTTGGA+AGG + chr4_1:71225396-71225415 Msa0569370:CDS 40.0%
TGGAATACTTGGACTTGGAA+GGG + chr4_1:71225397-71225416 Msa0569370:CDS 40.0%
!!! CTGAACTTGTTTTTGGAGGA+AGG + chr4_1:71225703-71225722 Msa0569370:CDS 40.0%
AGAATGGACACTATAGTCCT+TGG - chr4_1:71226109-71226128 None:intergenic 40.0%
!!! TCATCAGGTGCTGCTTTTAT+TGG - chr4_1:71226433-71226452 None:intergenic 40.0%
ATAGGTAAAGTCTGGTCATC+AGG - chr4_1:71226448-71226467 None:intergenic 40.0%
ACACTTAGTTTCACCAATGG+TGG + chr4_1:71226532-71226551 Msa0569370:CDS 40.0%
AGGTTTAACTCTTCCACCAT+TGG - chr4_1:71226548-71226567 None:intergenic 40.0%
GTTTGTCTAGGCATTCTGAA+CGG + chr4_1:71226724-71226743 Msa0569370:CDS 40.0%
TTGGGTGAACTGAACCTAAT+TGG + chr4_1:71226760-71226779 Msa0569370:CDS 40.0%
! ACACCTTCAGTTTGAAGTGT+TGG + chr4_1:71226919-71226938 Msa0569370:intron 40.0%
ATCCATGCTAGACAAAGTGA+TGG + chr4_1:71227059-71227078 Msa0569370:CDS 40.0%
TACCATCACTTTGTCTAGCA+TGG - chr4_1:71227064-71227083 None:intergenic 40.0%
GGAATACTATTGCAATCTGC+AGG - chr4_1:71227120-71227139 None:intergenic 40.0%
CATATTATAGAGCAGGGTGA+TGG + chr4_1:71227233-71227252 Msa0569370:three_prime_UTR 40.0%
ATTGGGGTAAGCTATTATGC+AGG + chr4_1:71227331-71227350 Msa0569370:three_prime_UTR 40.0%
TTGGGGTAAGCTATTATGCA+GGG + chr4_1:71227332-71227351 Msa0569370:three_prime_UTR 40.0%
GAAGATGAAGATAGGTAGGG+TGG + chr4_1:71223410-71223429 Msa0569370:exon 45.0%
AGATAGGTAGGGTGGGATTA+AGG + chr4_1:71223418-71223437 Msa0569370:CDS 45.0%
GATAGGTAGGGTGGGATTAA+GGG + chr4_1:71223419-71223438 Msa0569370:CDS 45.0%
!! GAGACTTGGTCTTGAAGACA+TGG - chr4_1:71223550-71223569 None:intergenic 45.0%
! TTAGGCCCTTTTCTGTTGCT+TGG + chr4_1:71223901-71223920 Msa0569370:intron 45.0%
ACCTCTTCTGACATTGATGG+GGG + chr4_1:71224005-71224024 Msa0569370:intron 45.0%
ATGAGGTTCGCTACTTCAAC+CGG - chr4_1:71224159-71224178 None:intergenic 45.0%
TCAAGAAAATAGGGCCTTGG+TGG - chr4_1:71224261-71224280 None:intergenic 45.0%
TCCTTGTACCCAATGTTCTG+AGG + chr4_1:71224323-71224342 Msa0569370:CDS 45.0%
ACCTCAGAACATTGGGTACA+AGG - chr4_1:71224327-71224346 None:intergenic 45.0%
TCATTGCTAGGCTTGTAGAG+TGG - chr4_1:71224651-71224670 None:intergenic 45.0%
GCACAAAGTGGATCTTTGCA+GGG - chr4_1:71224681-71224700 None:intergenic 45.0%
TCACATGTGTAGTCATCGGT+GGG - chr4_1:71224714-71224733 None:intergenic 45.0%
TTCACATGTGTAGTCATCGG+TGG - chr4_1:71224715-71224734 None:intergenic 45.0%
GCTGATCAATACTCAACCCT+TGG + chr4_1:71224768-71224787 Msa0569370:CDS 45.0%
CTCCATTGGTGAAGTTGAGA+AGG - chr4_1:71224815-71224834 None:intergenic 45.0%
ATGCCTAGACGACTACACAA+TGG + chr4_1:71225074-71225093 Msa0569370:intron 45.0%
! ATGCATTGGCGTTTGTGGAA+TGG - chr4_1:71225177-71225196 None:intergenic 45.0%
! AGATCATGCATTGGCGTTTG+TGG - chr4_1:71225182-71225201 None:intergenic 45.0%
GGAATACTTGGACTTGGAAG+GGG + chr4_1:71225398-71225417 Msa0569370:CDS 45.0%
ACAGTTGAATAGTCAGGGCT+TGG + chr4_1:71225439-71225458 Msa0569370:CDS 45.0%
ATTGTTTAAGCTCGCGAGGA+GGG + chr4_1:71225480-71225499 Msa0569370:CDS 45.0%
AGGGGAAAACATTACTCTGC+AGG + chr4_1:71225675-71225694 Msa0569370:intron 45.0%
!! GGACCAGCTGAACTTGTTTT+TGG + chr4_1:71225696-71225715 Msa0569370:CDS 45.0%
CCTCCAAAAACAAGTTCAGC+TGG - chr4_1:71225702-71225721 None:intergenic 45.0%
!!! CCAGCTGAACTTGTTTTTGG+AGG + chr4_1:71225699-71225718 Msa0569370:CDS 45.0%
!! GTTTTTGGAGGAAGGAAGAC+CGG + chr4_1:71225711-71225730 Msa0569370:CDS 45.0%
!! TTTTTGGAGGAAGGAAGACC+GGG + chr4_1:71225712-71225731 Msa0569370:CDS 45.0%
GCTGACGCTTAACACTTTCA+AGG - chr4_1:71225919-71225938 None:intergenic 45.0%
! TGATGCGCCATACTGACTAA+CGG - chr4_1:71226034-71226053 None:intergenic 45.0%
ACCAGACTTTACCTATGTGC+TGG + chr4_1:71226452-71226471 Msa0569370:CDS 45.0%
! ACTTTACCTATGTGCTGGCA+TGG + chr4_1:71226457-71226476 Msa0569370:CDS 45.0%
! CTTTTACCATGCCAGCACAT+AGG - chr4_1:71226466-71226485 None:intergenic 45.0%
GTTCACCCAATCCTACTTCA+GGG - chr4_1:71226750-71226769 None:intergenic 45.0%
AGTTCACCCAATCCTACTTC+AGG - chr4_1:71226751-71226770 None:intergenic 45.0%
GGTGAACTGAACCTAATTGG+AGG + chr4_1:71226763-71226782 Msa0569370:CDS 45.0%
! CTTCAAACTGAAGGTGTGTC+TGG - chr4_1:71226916-71226935 None:intergenic 45.0%
GCACCAACACTTCAAACTGA+AGG - chr4_1:71226925-71226944 None:intergenic 45.0%
GACAACGAGAAGCAGTTGAT+TGG + chr4_1:71227087-71227106 Msa0569370:CDS 45.0%
ACGAGAAGCAGTTGATTGGA+TGG + chr4_1:71227091-71227110 Msa0569370:CDS 45.0%
CGAGAAGCAGTTGATTGGAT+GGG + chr4_1:71227092-71227111 Msa0569370:CDS 45.0%
GAGAAGCAGTTGATTGGATG+GGG + chr4_1:71227093-71227112 Msa0569370:CDS 45.0%
! TGGGAGAGCGTGTGAATGAA+TGG - chr4_1:71223009-71223028 None:intergenic 50.0%
GGTGGGATTAAGGGTGATGA+TGG + chr4_1:71223428-71223447 Msa0569370:CDS 50.0%
CTTGAAGACATGGCTTCACC+TGG - chr4_1:71223540-71223559 None:intergenic 50.0%
GCAGCATGGTTCATGAGACT+TGG - chr4_1:71223564-71223583 None:intergenic 50.0%
AGTCTCATGAACCATGCTGC+TGG + chr4_1:71223564-71223583 Msa0569370:CDS 50.0%
CAATTGAGGAACCAGCAGCA+TGG - chr4_1:71223578-71223597 None:intergenic 50.0%
TCCCCCATCAATGTCAGAAG+AGG - chr4_1:71224009-71224028 None:intergenic 50.0%
CTTCTGACATTGATGGGGGA+AGG + chr4_1:71224009-71224028 Msa0569370:intron 50.0%
TCAACATAGGCCAACCACCA+AGG + chr4_1:71224244-71224263 Msa0569370:CDS 50.0%
! GAAAATAGGGCCTTGGTGGT+TGG - chr4_1:71224257-71224276 None:intergenic 50.0%
! ACACTGGTAGCGAACTCACA+TGG + chr4_1:71224286-71224305 Msa0569370:CDS 50.0%
!! GGGTACAAGGAGCATCACAT+TGG - chr4_1:71224314-71224333 None:intergenic 50.0%
CTAGGCTTGTAGAGTGGATG+GGG - chr4_1:71224645-71224664 None:intergenic 50.0%
GCTAGGCTTGTAGAGTGGAT+GGG - chr4_1:71224646-71224665 None:intergenic 50.0%
TGCTAGGCTTGTAGAGTGGA+TGG - chr4_1:71224647-71224666 None:intergenic 50.0%
CGCACAAAGTGGATCTTTGC+AGG - chr4_1:71224682-71224701 None:intergenic 50.0%
CGACCATTGTGTAGTCGTCT+AGG - chr4_1:71225080-71225099 None:intergenic 50.0%
ACTTGGACTTGGAAGGGGAA+AGG + chr4_1:71225403-71225422 Msa0569370:CDS 50.0%
!! GGCTTGGTGCGAAATGTGAT+GGG + chr4_1:71225455-71225474 Msa0569370:CDS 50.0%
GGACATTGTTTAAGCTCGCG+AGG + chr4_1:71225476-71225495 Msa0569370:CDS 50.0%
CATTGTTTAAGCTCGCGAGG+AGG + chr4_1:71225479-71225498 Msa0569370:CDS 50.0%
TTGTTTAAGCTCGCGAGGAG+GGG + chr4_1:71225481-71225500 Msa0569370:CDS 50.0%
GGAGGGGGATACATCTTCTT+TGG + chr4_1:71225497-71225516 Msa0569370:CDS 50.0%
!! TTTTGGAGGAAGGAAGACCG+GGG + chr4_1:71225713-71225732 Msa0569370:CDS 50.0%
GTGCCTTCCGTTAGTCAGTA+TGG + chr4_1:71226024-71226043 Msa0569370:intron 50.0%
GCCAGCACATAGGTAAAGTC+TGG - chr4_1:71226456-71226475 None:intergenic 50.0%
TCAGGAGGGATCTCAAACTG+AGG - chr4_1:71226568-71226587 None:intergenic 50.0%
ATTCTGAACGGCCCTGAAGT+AGG + chr4_1:71226736-71226755 Msa0569370:CDS 50.0%
AACGGCCCTGAAGTAGGATT+GGG + chr4_1:71226742-71226761 Msa0569370:CDS 50.0%
!! GGGCTTGGTGCGAAATGTGA+TGG + chr4_1:71225454-71225473 Msa0569370:CDS 55.0%
TGTTTAAGCTCGCGAGGAGG+GGG + chr4_1:71225482-71225501 Msa0569370:CDS 55.0%
! GCGCCATACTGACTAACGGA+AGG - chr4_1:71226030-71226049 None:intergenic 55.0%
GAACGGCCCTGAAGTAGGAT+TGG + chr4_1:71226741-71226760 Msa0569370:CDS 55.0%
TCTGGTGTGCGAGACATGTC+TGG - chr4_1:71226898-71226917 None:intergenic 55.0%
GGCTGCAAGGACGCACAAAG+TGG - chr4_1:71224693-71224712 None:intergenic 60.0%
!! GTAGTCATCGGTGGGCTGCA+AGG - chr4_1:71224706-71224725 None:intergenic 60.0%
GGAGGAAGGAAGACCGGGGT+TGG + chr4_1:71225717-71225736 Msa0569370:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 71222942 71227433 71222942 ID=Msa0569370;Name=Msa0569370
chr4_1 mRNA 71222942 71227433 71222942 ID=Msa0569370-mRNA-1;Parent=Msa0569370;Name=Msa0569370-mRNA-1;_AED=0.05;_eAED=0.05;_QI=472|0.85|0.87|1|1|1|8|269|412
chr4_1 exon 71222942 71223631 71222942 ID=Msa0569370-mRNA-1:exon:24120;Parent=Msa0569370-mRNA-1
chr4_1 exon 71224227 71224344 71224227 ID=Msa0569370-mRNA-1:exon:24121;Parent=Msa0569370-mRNA-1
chr4_1 exon 71224711 71224862 71224711 ID=Msa0569370-mRNA-1:exon:24122;Parent=Msa0569370-mRNA-1
chr4_1 exon 71225349 71225573 71225349 ID=Msa0569370-mRNA-1:exon:24123;Parent=Msa0569370-mRNA-1
chr4_1 exon 71225677 71225818 71225677 ID=Msa0569370-mRNA-1:exon:24124;Parent=Msa0569370-mRNA-1
chr4_1 exon 71226406 71226606 71226406 ID=Msa0569370-mRNA-1:exon:24125;Parent=Msa0569370-mRNA-1
chr4_1 exon 71226712 71226784 71226712 ID=Msa0569370-mRNA-1:exon:24126;Parent=Msa0569370-mRNA-1
chr4_1 exon 71227055 71227433 71227055 ID=Msa0569370-mRNA-1:exon:24127;Parent=Msa0569370-mRNA-1
chr4_1 five_prime_UTR 71222942 71223413 71222942 ID=Msa0569370-mRNA-1:five_prime_utr;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71223414 71223631 71223414 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71224227 71224344 71224227 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71224711 71224862 71224711 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71225349 71225573 71225349 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71225677 71225818 71225677 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71226406 71226606 71226406 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71226712 71226784 71226712 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 CDS 71227055 71227164 71227055 ID=Msa0569370-mRNA-1:cds;Parent=Msa0569370-mRNA-1
chr4_1 three_prime_UTR 71227165 71227433 71227165 ID=Msa0569370-mRNA-1:three_prime_utr;Parent=Msa0569370-mRNA-1
Gene Sequence

>Msa0569370

ATGAAGATAGGTAGGGTGGGATTAAGGGTGATGATGGTTGCATTGATGATGATGATGTTAAACTTGTCTTCTTGTTCAGCTTGGTTTGATAATAAACGCAAAAGTGGAAGAAACTCAATTTTACCAGGTGAAGCCATGTCTTCAAGACCAAGTCTCATGAACCATGCTGCTGGTTCCTCAATTGTGTTCCCTATTTATGGCAACGTTTATCCTGTTGGGTTCTATAATGTTACTCTCAACATAGGCCAACCACCAAGGCCCTATTTTCTTGATGTTGACACTGGTAGCGAACTCACATGGCTCCAATGTGATGCTCCTTGTACCCAATGTTCTGAGCCCACCGATGACTACACATGTGAAGACCCTAATCAATGTGACTATGAAATTAAGTATGCTGATCAATACTCAACCCTTGGTGTACTTCTAAATGATGTTTACCTTCTCAACTTCACCAATGGAGTCCAACTTAAAGTTAGAATGGCACTTGGATGTGGATATGACCAGATATTTTCACCTTCAACTTACCACCCTTTAGATGGAATACTTGGACTTGGAAGGGGAAAGGCCAGCTTGATATCACAGTTGAATAGTCAGGGCTTGGTGCGAAATGTGATGGGACATTGTTTAAGCTCGCGAGGAGGGGGATACATCTTCTTTGGAAATGCTTATGATTCTTCTCGAATGAGTTGGACTCCAATTTCATCAATCGATTCGGGAAAACATTACTCTGCAGGACCAGCTGAACTTGTTTTTGGAGGAAGGAAGACCGGGGTTGGAAGTTTAAATATTATCTTTGACACTGGAAGTTCCTATACATACTTCAACTCTCAGGCTTACCAAGCAATGATTTCTTTGCTGAATAAGGAATTACATAGAAAGCCAATAAAAGCAGCACCTGATGACCAGACTTTACCTATGTGCTGGCATGGTAAAAGACCTTTCAGAAGCATAAATGAAGTGAAGAAATACTTCAAGCCACTAACACTTAGTTTCACCAATGGTGGAAGAGTTAAACCTCAGTTTGAGATCCCTCCTGAAGCTTATCTTATAATATCAAATATGGGAAATGTTTGTCTAGGCATTCTGAACGGCCCTGAAGTAGGATTGGGTGAACTGAACCTAATTGGAGACATATCCATGCTAGACAAAGTGATGGTATTCGACAACGAGAAGCAGTTGATTGGATGGGGACCTGCAGATTGCAATAGTATTCCAAAATCCAGAGATTTCAGTTTATGA
Protein sequence

>Msa0569370

MKIGRVGLRVMMVALMMMMLNLSSCSAWFDNKRKSGRNSILPGEAMSSRPSLMNHAAGSSIVFPIYGNVYPVGFYNVTLNIGQPPRPYFLDVDTGSELTWLQCDAPCTQCSEPTDDYTCEDPNQCDYEIKYADQYSTLGVLLNDVYLLNFTNGVQLKVRMALGCGYDQIFSPSTYHPLDGILGLGRGKASLISQLNSQGLVRNVMGHCLSSRGGGYIFFGNAYDSSRMSWTPISSIDSGKHYSAGPAELVFGGRKTGVGSLNIIFDTGSSYTYFNSQAYQAMISLLNKELHRKPIKAAPDDQTLPMCWHGKRPFRSINEVKKYFKPLTLSFTNGGRVKPQFEIPPEAYLIISNMGNVCLGILNGPEVGLGELNLIGDISMLDKVMVFDNEKQLIGWGPADCNSIPKSRDFSL*