AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610520


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610520 MtrunA17_Chr4g0043521 100.000 154 0 0 1 154 1 154 2.72e-115 325
Msa0610520 MtrunA17_Chr2g0291651 90.132 152 15 0 3 154 5 156 6.31e-105 299
Msa0610520 MtrunA17_Chr4g0010271 79.333 150 31 0 5 154 3 152 8.12e-93 268
Msa0610520 MtrunA17_Chr1g0169861 79.333 150 31 0 5 154 53 202 1.10e-91 267
Msa0610520 MtrunA17_Chr7g0237561 76.667 150 35 0 5 154 3 152 9.13e-88 255
Msa0610520 MtrunA17_Chr7g0237521 70.000 150 45 0 5 154 3 152 1.43e-80 237
Msa0610520 MtrunA17_Chr2g0296021 68.000 150 48 0 4 153 2 151 1.91e-78 232
Msa0610520 MtrunA17_Chr1g0210521 54.118 170 59 2 4 154 2 171 2.87e-68 207
Msa0610520 MtrunA17_Chr4g0010211 79.775 89 18 0 66 154 3 91 3.90e-49 154
Msa0610520 MtrunA17_Chr1g0169851 69.608 102 31 0 59 160 8 109 4.44e-49 155
Msa0610520 MtrunA17_Chr5g0441741 37.162 148 90 3 8 154 3 148 2.69e-29 107
Msa0610520 MtrunA17_Chr4g0026121 32.000 150 97 4 7 154 2 148 2.04e-27 102
Msa0610520 MtrunA17_Chr4g0064371 31.579 152 97 3 8 154 3 152 5.20e-23 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610520 AT1G04760.2 80.667 150 29 0 5 154 3 152 2.36e-92 267
Msa0610520 AT1G04760.1 80.667 150 29 0 5 154 3 152 2.36e-92 267
Msa0610520 AT2G32670.1 80.000 150 30 0 5 154 68 217 2.91e-91 267
Msa0610520 AT2G33120.1 80.000 150 30 0 5 154 3 152 3.46e-91 265
Msa0610520 AT1G04750.1 78.667 150 32 0 5 154 3 152 2.56e-90 262
Msa0610520 AT2G33120.2 75.316 158 31 1 5 154 3 160 1.15e-87 256
Msa0610520 AT2G33110.2 70.000 150 45 0 5 154 3 152 1.13e-79 234
Msa0610520 AT2G33110.1 70.000 150 45 0 5 154 3 152 7.73e-79 233
Msa0610520 AT4G15780.1 69.333 150 45 1 5 153 3 152 4.05e-77 228
Msa0610520 AT3G54300.3 56.140 171 54 2 4 154 2 171 5.25e-68 206
Msa0610520 AT3G54300.2 56.140 171 54 2 4 154 2 171 5.25e-68 206
Msa0610520 AT3G54300.1 56.140 171 54 2 4 154 2 171 5.25e-68 206
Msa0610520 AT1G04750.2 82.432 74 13 0 81 154 1 74 3.64e-40 132
Msa0610520 AT1G04750.4 82.432 74 13 0 81 154 1 74 3.64e-40 132
Msa0610520 AT1G04750.3 82.432 74 13 0 81 154 1 74 3.64e-40 132
Msa0610520 AT4G32150.1 37.584 149 90 3 7 154 2 148 3.50e-30 109
Msa0610520 AT5G11150.1 38.000 150 89 3 7 154 2 149 5.51e-30 108
Msa0610520 AT5G11150.2 38.000 150 89 3 7 154 2 149 9.74e-30 108
Msa0610520 AT5G22360.1 34.437 151 93 4 7 154 2 149 2.76e-27 102
Msa0610520 AT2G25340.1 33.557 149 96 3 7 154 2 148 2.78e-25 97.1

Find 37 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATATGTTCCTTCAATTT+TGG 0.127814 4_2:+58317333 None:intergenic
AACAGCTTGAATAAGGAATT+TGG 0.135464 4_2:-58317445 Msa0610520:CDS
TTCAATTACCTCGTCGATAA+TGG 0.314519 4_2:-58317869 Msa0610520:CDS
TGCACCTAACAGCTTGAATA+AGG 0.338054 4_2:-58317452 Msa0610520:CDS
ACAGCTTGAATAAGGAATTT+GGG 0.392757 4_2:-58317444 Msa0610520:intron
GCAAGCTTACTCACCTCCTC+CGG 0.394354 4_2:+58317294 None:intergenic
GTTGAAGTTTCCGCTGAATT+CGG 0.415620 4_2:+58317967 None:intergenic
AATTCCTTATTCAAGCTGTT+AGG 0.425518 4_2:+58317448 None:intergenic
TTGTGGCAGGCCAAAATTGA+AGG 0.466510 4_2:-58317343 Msa0610520:intron
CATGATGGAGAATATTGAAA+AGG 0.507884 4_2:-58317235 Msa0610520:CDS
TTGTGTTGTTGCGGATGAAA+CGG 0.512801 4_2:-58317560 Msa0610520:CDS
GCTTGCTAAGGTGAAGGCTC+AGG 0.546240 4_2:-58317277 Msa0610520:CDS
CAAGCTTACTCACCTCCTCC+GGG 0.549863 4_2:+58317295 None:intergenic
GTGAATTTGTTGTTAGACGC+TGG 0.551142 4_2:+58317914 None:intergenic
AACGGTTGGAAGACAAGTGC+CGG 0.560243 4_2:-58317542 Msa0610520:CDS
TTTGTTGCGAAATATGGTGG+TGG 0.569761 4_2:-58317490 Msa0610520:CDS
CTGAAGGCATTGAAAAGCGA+TGG 0.572755 4_2:+58317943 None:intergenic
ATTATCGACGAGGTAATTGA+AGG 0.574959 4_2:+58317871 None:intergenic
CTCTTAGGTTAAAGTAGAAG+AGG 0.575526 4_2:+58317209 None:intergenic
AAATTCACTTACAATTGTGA+TGG 0.582315 4_2:-58317899 Msa0610520:CDS
TTGTGTTGATCACCCGGAGG+AGG 0.583937 4_2:-58317307 Msa0610520:CDS
GCAGTATTGTGTTGATCACC+CGG 0.590880 4_2:-58317313 Msa0610520:CDS
TAGACGCTGGAAGCTTCTGA+AGG 0.592334 4_2:+58317927 None:intergenic
GAGTAAGCTTGCTAAGGTGA+AGG 0.597551 4_2:-58317283 Msa0610520:CDS
TTGTTGCGAAATATGGTGGT+GGG 0.599780 4_2:-58317489 Msa0610520:CDS
TGAAGTGAAAGGTGTCATGA+TGG 0.602006 4_2:-58317250 Msa0610520:CDS
GGAGGTGAGTAAGCTTGCTA+AGG 0.602293 4_2:-58317289 Msa0610520:CDS
GTTGTTGCGGATGAAACGGT+TGG 0.620353 4_2:-58317556 Msa0610520:CDS
GCTCAGGTGTCTGAAGTGAA+AGG 0.620623 4_2:-58317261 Msa0610520:CDS
TGTAGCGTATTGTGTTGTTG+CGG 0.643271 4_2:-58317569 Msa0610520:intron
TGCGAAATATGGTGGTGGGA+AGG 0.648574 4_2:-58317485 Msa0610520:CDS
TCTGAATACACCGAATTCAG+CGG 0.660683 4_2:-58317977 Msa0610520:CDS
ATCTACGCTTTCGTTTCTCG+CGG 0.661937 4_2:-58318013 Msa0610520:CDS
GGTTGGAAGACAAGTGCCGG+TGG 0.663656 4_2:-58317539 Msa0610520:CDS
ACACGTTCCAGAAAAGCCAC+CGG 0.669559 4_2:+58317523 None:intergenic
AACTGTAGCCATTATCGACG+AGG 0.726012 4_2:+58317861 None:intergenic
GTATTGTGTTGATCACCCGG+AGG 0.776651 4_2:-58317310 Msa0610520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATTAAAAGTGATTTT+AGG - chr4_2:58317553-58317572 Msa0610520:CDS 10.0%
!!! TTATTATTAAAAGTGATTTT+AGG - chr4_2:58317553-58317572 Msa0610520:CDS 10.0%
!! TACTGATATAACTAATTTCT+AGG + chr4_2:58317514-58317533 None:intergenic 20.0%
!!! ATTAAAAGTGATTTTAGGTT+AGG - chr4_2:58317558-58317577 Msa0610520:CDS 20.0%
!!! TTAAAAGTGATTTTAGGTTA+GGG - chr4_2:58317559-58317578 Msa0610520:CDS 20.0%
!! TGTGATTTGATATTTAATTG+TGG - chr4_2:58317889-58317908 Msa0610520:CDS 20.0%
!! TACTGATATAACTAATTTCT+AGG + chr4_2:58317514-58317533 None:intergenic 20.0%
!!! ATTAAAAGTGATTTTAGGTT+AGG - chr4_2:58317558-58317577 Msa0610520:CDS 20.0%
!!! TTAAAAGTGATTTTAGGTTA+GGG - chr4_2:58317559-58317578 Msa0610520:CDS 20.0%
!! TGTGATTTGATATTTAATTG+TGG - chr4_2:58317889-58317908 Msa0610520:CDS 20.0%
! AAATTCACTTACAATTGTGA+TGG - chr4_2:58317350-58317369 Msa0610520:intron 25.0%
!! TGAGTTTATGATGATGATTT+AGG - chr4_2:58317831-58317850 Msa0610520:intron 25.0%
!! GAGTTTATGATGATGATTTA+GGG - chr4_2:58317832-58317851 Msa0610520:intron 25.0%
! ATTTGATATTTAATTGTGGC+AGG - chr4_2:58317893-58317912 Msa0610520:CDS 25.0%
! AAATTCACTTACAATTGTGA+TGG - chr4_2:58317350-58317369 Msa0610520:intron 25.0%
!! TGAGTTTATGATGATGATTT+AGG - chr4_2:58317831-58317850 Msa0610520:intron 25.0%
!! GAGTTTATGATGATGATTTA+GGG - chr4_2:58317832-58317851 Msa0610520:intron 25.0%
! ATTTGATATTTAATTGTGGC+AGG - chr4_2:58317893-58317912 Msa0610520:CDS 25.0%
! GATGATTTTGTTGCGAAATA+TGG - chr4_2:58317753-58317772 Msa0610520:intron 30.0%
AATTCCTTATTCAAGCTGTT+AGG + chr4_2:58317804-58317823 None:intergenic 30.0%
AACAGCTTGAATAAGGAATT+TGG - chr4_2:58317804-58317823 Msa0610520:intron 30.0%
ACAGCTTGAATAAGGAATTT+GGG - chr4_2:58317805-58317824 Msa0610520:intron 30.0%
!!! TTGCGATTTTTGTTGTTGAT+GGG - chr4_2:58317859-58317878 Msa0610520:intron 30.0%
! TGCATATGTTCCTTCAATTT+TGG + chr4_2:58317919-58317938 None:intergenic 30.0%
! CATGATGGAGAATATTGAAA+AGG - chr4_2:58318014-58318033 Msa0610520:CDS 30.0%
! GATGATTTTGTTGCGAAATA+TGG - chr4_2:58317753-58317772 Msa0610520:intron 30.0%
AATTCCTTATTCAAGCTGTT+AGG + chr4_2:58317804-58317823 None:intergenic 30.0%
AACAGCTTGAATAAGGAATT+TGG - chr4_2:58317804-58317823 Msa0610520:intron 30.0%
ACAGCTTGAATAAGGAATTT+GGG - chr4_2:58317805-58317824 Msa0610520:intron 30.0%
!!! TTGCGATTTTTGTTGTTGAT+GGG - chr4_2:58317859-58317878 Msa0610520:intron 30.0%
! TGCATATGTTCCTTCAATTT+TGG + chr4_2:58317919-58317938 None:intergenic 30.0%
! CATGATGGAGAATATTGAAA+AGG - chr4_2:58318014-58318033 Msa0610520:CDS 30.0%
! AAGCGTAGATCAGAGATTTT+TGG + chr4_2:58317226-58317245 None:intergenic 35.0%
ATTATCGACGAGGTAATTGA+AGG + chr4_2:58317381-58317400 None:intergenic 35.0%
TTCAATTACCTCGTCGATAA+TGG - chr4_2:58317380-58317399 Msa0610520:intron 35.0%
! GATTTTGTTGCGAAATATGG+TGG - chr4_2:58317756-58317775 Msa0610520:intron 35.0%
!!! GTTGCGATTTTTGTTGTTGA+TGG - chr4_2:58317858-58317877 Msa0610520:intron 35.0%
! AAGCGTAGATCAGAGATTTT+TGG + chr4_2:58317226-58317245 None:intergenic 35.0%
ATTATCGACGAGGTAATTGA+AGG + chr4_2:58317381-58317400 None:intergenic 35.0%
TTCAATTACCTCGTCGATAA+TGG - chr4_2:58317380-58317399 Msa0610520:intron 35.0%
! GATTTTGTTGCGAAATATGG+TGG - chr4_2:58317756-58317775 Msa0610520:intron 35.0%
!!! GTTGCGATTTTTGTTGTTGA+TGG - chr4_2:58317858-58317877 Msa0610520:intron 35.0%
TCTGAATACACCGAATTCAG+CGG - chr4_2:58317272-58317291 Msa0610520:CDS 40.0%
GTTGAAGTTTCCGCTGAATT+CGG + chr4_2:58317285-58317304 None:intergenic 40.0%
!! GTGAATTTGTTGTTAGACGC+TGG + chr4_2:58317338-58317357 None:intergenic 40.0%
GAACTACGATTACGCTAGAT+CGG + chr4_2:58317462-58317481 None:intergenic 40.0%
! TGTAGCGTATTGTGTTGTTG+CGG - chr4_2:58317680-58317699 Msa0610520:intron 40.0%
! TTGTGTTGTTGCGGATGAAA+CGG - chr4_2:58317689-58317708 Msa0610520:intron 40.0%
TTTGTTGCGAAATATGGTGG+TGG - chr4_2:58317759-58317778 Msa0610520:intron 40.0%
TTGTTGCGAAATATGGTGGT+GGG - chr4_2:58317760-58317779 Msa0610520:intron 40.0%
TGCACCTAACAGCTTGAATA+AGG - chr4_2:58317797-58317816 Msa0610520:intron 40.0%
!! TGAAGTGAAAGGTGTCATGA+TGG - chr4_2:58317999-58318018 Msa0610520:CDS 40.0%
TCTGAATACACCGAATTCAG+CGG - chr4_2:58317272-58317291 Msa0610520:CDS 40.0%
GTTGAAGTTTCCGCTGAATT+CGG + chr4_2:58317285-58317304 None:intergenic 40.0%
!! GTGAATTTGTTGTTAGACGC+TGG + chr4_2:58317338-58317357 None:intergenic 40.0%
GAACTACGATTACGCTAGAT+CGG + chr4_2:58317462-58317481 None:intergenic 40.0%
! TGTAGCGTATTGTGTTGTTG+CGG - chr4_2:58317680-58317699 Msa0610520:intron 40.0%
! TTGTGTTGTTGCGGATGAAA+CGG - chr4_2:58317689-58317708 Msa0610520:intron 40.0%
TTTGTTGCGAAATATGGTGG+TGG - chr4_2:58317759-58317778 Msa0610520:intron 40.0%
TTGTTGCGAAATATGGTGGT+GGG - chr4_2:58317760-58317779 Msa0610520:intron 40.0%
TGCACCTAACAGCTTGAATA+AGG - chr4_2:58317797-58317816 Msa0610520:intron 40.0%
!! TGAAGTGAAAGGTGTCATGA+TGG - chr4_2:58317999-58318018 Msa0610520:CDS 40.0%
ATCTACGCTTTCGTTTCTCG+CGG - chr4_2:58317236-58317255 Msa0610520:CDS 45.0%
!! CTGAAGGCATTGAAAAGCGA+TGG + chr4_2:58317309-58317328 None:intergenic 45.0%
AACTGTAGCCATTATCGACG+AGG + chr4_2:58317391-58317410 None:intergenic 45.0%
TTGTGGCAGGCCAAAATTGA+AGG - chr4_2:58317906-58317925 Msa0610520:CDS 45.0%
! GCAGTATTGTGTTGATCACC+CGG - chr4_2:58317936-58317955 Msa0610520:CDS 45.0%
!! GAGTAAGCTTGCTAAGGTGA+AGG - chr4_2:58317966-58317985 Msa0610520:CDS 45.0%
ATCTACGCTTTCGTTTCTCG+CGG - chr4_2:58317236-58317255 Msa0610520:CDS 45.0%
!! CTGAAGGCATTGAAAAGCGA+TGG + chr4_2:58317309-58317328 None:intergenic 45.0%
AACTGTAGCCATTATCGACG+AGG + chr4_2:58317391-58317410 None:intergenic 45.0%
TTGTGGCAGGCCAAAATTGA+AGG - chr4_2:58317906-58317925 Msa0610520:CDS 45.0%
! GCAGTATTGTGTTGATCACC+CGG - chr4_2:58317936-58317955 Msa0610520:CDS 45.0%
!! GAGTAAGCTTGCTAAGGTGA+AGG - chr4_2:58317966-58317985 Msa0610520:CDS 45.0%
TAGACGCTGGAAGCTTCTGA+AGG + chr4_2:58317325-58317344 None:intergenic 50.0%
GTTGTTGCGGATGAAACGGT+TGG - chr4_2:58317693-58317712 Msa0610520:intron 50.0%
AACGGTTGGAAGACAAGTGC+CGG - chr4_2:58317707-58317726 Msa0610520:intron 50.0%
ACACGTTCCAGAAAAGCCAC+CGG + chr4_2:58317729-58317748 None:intergenic 50.0%
!!! GGCTTTTCTGGAACGTGTGA+AGG - chr4_2:58317731-58317750 Msa0610520:intron 50.0%
!! TGCGAAATATGGTGGTGGGA+AGG - chr4_2:58317764-58317783 Msa0610520:intron 50.0%
! GTATTGTGTTGATCACCCGG+AGG - chr4_2:58317939-58317958 Msa0610520:CDS 50.0%
!! GGAGGTGAGTAAGCTTGCTA+AGG - chr4_2:58317960-58317979 Msa0610520:CDS 50.0%
! GCTCAGGTGTCTGAAGTGAA+AGG - chr4_2:58317988-58318007 Msa0610520:CDS 50.0%
TAGACGCTGGAAGCTTCTGA+AGG + chr4_2:58317325-58317344 None:intergenic 50.0%
GTTGTTGCGGATGAAACGGT+TGG - chr4_2:58317693-58317712 Msa0610520:intron 50.0%
AACGGTTGGAAGACAAGTGC+CGG - chr4_2:58317707-58317726 Msa0610520:intron 50.0%
ACACGTTCCAGAAAAGCCAC+CGG + chr4_2:58317729-58317748 None:intergenic 50.0%
!!! GGCTTTTCTGGAACGTGTGA+AGG - chr4_2:58317731-58317750 Msa0610520:intron 50.0%
!! TGCGAAATATGGTGGTGGGA+AGG - chr4_2:58317764-58317783 Msa0610520:intron 50.0%
! GTATTGTGTTGATCACCCGG+AGG - chr4_2:58317939-58317958 Msa0610520:CDS 50.0%
!! GGAGGTGAGTAAGCTTGCTA+AGG - chr4_2:58317960-58317979 Msa0610520:CDS 50.0%
! GCTCAGGTGTCTGAAGTGAA+AGG - chr4_2:58317988-58318007 Msa0610520:CDS 50.0%
!!! ACAAGTGCCGGTGGCTTTTC+TGG - chr4_2:58317719-58317738 Msa0610520:intron 55.0%
! TTGTGTTGATCACCCGGAGG+AGG - chr4_2:58317942-58317961 Msa0610520:CDS 55.0%
CAAGCTTACTCACCTCCTCC+GGG + chr4_2:58317957-58317976 None:intergenic 55.0%
GCAAGCTTACTCACCTCCTC+CGG + chr4_2:58317958-58317977 None:intergenic 55.0%
! GCTTGCTAAGGTGAAGGCTC+AGG - chr4_2:58317972-58317991 Msa0610520:CDS 55.0%
!!! ACAAGTGCCGGTGGCTTTTC+TGG - chr4_2:58317719-58317738 Msa0610520:intron 55.0%
! TTGTGTTGATCACCCGGAGG+AGG - chr4_2:58317942-58317961 Msa0610520:CDS 55.0%
CAAGCTTACTCACCTCCTCC+GGG + chr4_2:58317957-58317976 None:intergenic 55.0%
GCAAGCTTACTCACCTCCTC+CGG + chr4_2:58317958-58317977 None:intergenic 55.0%
! GCTTGCTAAGGTGAAGGCTC+AGG - chr4_2:58317972-58317991 Msa0610520:CDS 55.0%
!! GGTTGGAAGACAAGTGCCGG+TGG - chr4_2:58317710-58317729 Msa0610520:intron 60.0%
!! GGTTGGAAGACAAGTGCCGG+TGG - chr4_2:58317710-58317729 Msa0610520:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58317212 58318059 58317212 ID=Msa0610520;Name=Msa0610520
chr4_2 mRNA 58317212 58318059 58317212 ID=Msa0610520-mRNA-1;Parent=Msa0610520;Name=Msa0610520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|3|0|160
chr4_2 exon 58317864 58318059 58317864 ID=Msa0610520-mRNA-1:exon:15294;Parent=Msa0610520-mRNA-1
chr4_2 exon 58317445 58317586 58317445 ID=Msa0610520-mRNA-1:exon:15293;Parent=Msa0610520-mRNA-1
chr4_2 exon 58317212 58317356 58317212 ID=Msa0610520-mRNA-1:exon:15292;Parent=Msa0610520-mRNA-1
chr4_2 CDS 58317864 58318059 58317864 ID=Msa0610520-mRNA-1:cds;Parent=Msa0610520-mRNA-1
chr4_2 CDS 58317445 58317586 58317445 ID=Msa0610520-mRNA-1:cds;Parent=Msa0610520-mRNA-1
chr4_2 CDS 58317212 58317356 58317212 ID=Msa0610520-mRNA-1:cds;Parent=Msa0610520-mRNA-1
Gene Sequence

>Msa0610520

ATGGGACAGAACCAAAAATCTCTGATCTACGCTTTCGTTTCTCGCGGAAGCGTGATTCTCTCTGAATACACCGAATTCAGCGGAAACTTCAACTCCATCGCTTTTCAATGCCTTCAGAAGCTTCCAGCGTCTAACAACAAATTCACTTACAATTGTGATGGTCACACCTTCAATTACCTCGTCGATAATGGCTACACGTATTGTGTTGTTGCGGATGAAACGGTTGGAAGACAAGTGCCGGTGGCTTTTCTGGAACGTGTGAAGGATGATTTTGTTGCGAAATATGGTGGTGGGAAGGCGTCTACAGCTGCACCTAACAGCTTGAATAAGGAATTTGGGCCAAAATTGAAGGAACATATGCAGTATTGTGTTGATCACCCGGAGGAGGTGAGTAAGCTTGCTAAGGTGAAGGCTCAGGTGTCTGAAGTGAAAGGTGTCATGATGGAGAATATTGAAAAGGTATCCTCTTCTACTTTAACCTAA

Protein sequence

>Msa0610520

MGQNQKSLIYAFVSRGSVILSEYTEFSGNFNSIAFQCLQKLPASNNKFTYNCDGHTFNYLVDNGYTYCVVADETVGRQVPVAFLERVKDDFVAKYGGGKASTAAPNSLNKEFGPKLKEHMQYCVDHPEEVSKLAKVKAQVSEVKGVMMENIEKVSSSTLT*