AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1184690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184690 MtrunA17_Chr8g0350031 86.400 125 6 1 1 114 24 148 1.96e-74 217
Msa1184690 MtrunA17_Chr8g0350021 77.600 125 17 1 1 114 24 148 2.85e-68 201
Msa1184690 MtrunA17_Chr1g0147471 66.129 62 21 0 2 63 1 62 7.53e-28 97.1
Msa1184690 MtrunA17_Chr5g0435251 39.695 131 53 4 2 110 25 151 8.71e-26 94.4
Msa1184690 MtrunA17_Chr5g0435261 38.636 132 55 4 3 111 27 155 2.28e-24 90.9
Msa1184690 MtrunA17_Chr7g0246521 58.491 53 22 0 2 54 1 53 1.26e-20 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184690 AT1G63220.1 46.774 124 54 2 2 114 25 147 1.35e-32 111
Msa1184690 AT1G63220.2 44.444 126 52 3 6 114 9 133 1.45e-27 98.6
Msa1184690 AT3G55470.2 32.090 134 66 4 2 113 25 155 9.83e-15 66.2

Find 21 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATAATGATGATAATAT+TGG 0.281672 8_1:-20587979 Msa1184690:CDS
AGAATATTGTGGAGAGATTA+AGG 0.301540 8_1:-20587428 Msa1184690:CDS
TGAGCTCAATCTAAAGATAA+TGG 0.346296 8_1:-20588013 Msa1184690:CDS
ACAAGATTGTAAGAATTCTC+TGG 0.392769 8_1:+20587460 None:intergenic
GCTCCTGTGCTTTGTAACTA+AGG 0.446848 8_1:+20588213 None:intergenic
GAAAAGTCTCATTCCATTGA+GGG 0.461869 8_1:+20588061 None:intergenic
CAATCTAAAGATAATGGAAA+AGG 0.492449 8_1:-20588007 Msa1184690:CDS
GTGCTGGATCTAACCCTCAA+TGG 0.522113 8_1:-20588074 Msa1184690:CDS
AGAAAAGTCTCATTCCATTG+AGG 0.522164 8_1:+20588060 None:intergenic
AAGAGGAGGAGACTGGTGGA+TGG 0.524498 8_1:-20587259 Msa1184690:CDS
TACAATGAAGAGGAGGAGAC+TGG 0.545609 8_1:-20587266 Msa1184690:CDS
TTGTAACTAAGGATCACATA+AGG 0.555757 8_1:+20588224 None:intergenic
CACAAGAGTTCTGTGCAAGA+AGG 0.570141 8_1:-20588191 Msa1184690:intron
GTGAAGGATGAAGAATATTG+TGG 0.592520 8_1:-20587439 Msa1184690:CDS
GATCCTTAGTTACAAAGCAC+AGG 0.599886 8_1:-20588216 Msa1184690:CDS
TGATCAGAGTTACAATGAAG+AGG 0.605643 8_1:-20587276 Msa1184690:CDS
GAATTCTTACAATCTTGTGA+AGG 0.613624 8_1:-20587455 Msa1184690:CDS
TCAGAGTTACAATGAAGAGG+AGG 0.621822 8_1:-20587273 Msa1184690:CDS
AATGAAGAGGAGGAGACTGG+TGG 0.640663 8_1:-20587263 Msa1184690:CDS
GATTGTAAGAATTCTCTGGA+AGG 0.642476 8_1:+20587464 None:intergenic
ATATTGTGGAGAGATTAAGG+TGG 0.788819 8_1:-20587425 Msa1184690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAATGATGATAATAT+TGG - chr8_1:20587492-20587511 Msa1184690:intron 10.0%
!! TATAATAATGATGATAATAT+TGG - chr8_1:20587492-20587511 Msa1184690:intron 10.0%
!!! ATATTTTGATGAATAATATC+AGG - chr8_1:20587375-20587394 Msa1184690:intron 15.0%
!!! ATTGATAGTACATTTTAATA+GGG + chr8_1:20588155-20588174 None:intergenic 15.0%
!!! AATTGATAGTACATTTTAAT+AGG + chr8_1:20588156-20588175 None:intergenic 15.0%
!! TAAAATGTACTATCAATTAT+TGG - chr8_1:20588158-20588177 Msa1184690:intron 15.0%
!!! ATATTTTGATGAATAATATC+AGG - chr8_1:20587375-20587394 Msa1184690:intron 15.0%
!!! ATTGATAGTACATTTTAATA+GGG + chr8_1:20588155-20588174 None:intergenic 15.0%
!!! AATTGATAGTACATTTTAAT+AGG + chr8_1:20588156-20588175 None:intergenic 15.0%
!! TAAAATGTACTATCAATTAT+TGG - chr8_1:20588158-20588177 Msa1184690:intron 15.0%
!!! AAACATAAGTAGTATTGATT+TGG - chr8_1:20587315-20587334 Msa1184690:intron 20.0%
!!! TTTTTGCTTATATTTCATTC+TGG + chr8_1:20587643-20587662 None:intergenic 20.0%
!! AGAATGAAATATAAGCAAAA+AGG - chr8_1:20587642-20587661 Msa1184690:intron 20.0%
!! GAATGAAATATAAACAAAAG+TGG + chr8_1:20587719-20587738 None:intergenic 20.0%
!!! CTTTTGTTTATATTTCATTC+CGG - chr8_1:20587719-20587738 Msa1184690:intron 20.0%
!! GTTTAAAAATTTACACCTAA+AGG - chr8_1:20587798-20587817 Msa1184690:intron 20.0%
!!! AAACATAAGTAGTATTGATT+TGG - chr8_1:20587315-20587334 Msa1184690:intron 20.0%
!!! TTTTTGCTTATATTTCATTC+TGG + chr8_1:20587643-20587662 None:intergenic 20.0%
!! AGAATGAAATATAAGCAAAA+AGG - chr8_1:20587642-20587661 Msa1184690:intron 20.0%
!! GAATGAAATATAAACAAAAG+TGG + chr8_1:20587719-20587738 None:intergenic 20.0%
!!! CTTTTGTTTATATTTCATTC+CGG - chr8_1:20587719-20587738 Msa1184690:intron 20.0%
!! GTTTAAAAATTTACACCTAA+AGG - chr8_1:20587798-20587817 Msa1184690:intron 20.0%
!!! TTTGTTATTTTGTTCTCTAG+TGG - chr8_1:20587341-20587360 Msa1184690:intron 25.0%
!!! CAATCTAAAGATAATGGAAA+AGG - chr8_1:20587464-20587483 Msa1184690:CDS 25.0%
!! TGTCTTTGATTCATCTTTTT+AGG - chr8_1:20587554-20587573 Msa1184690:intron 25.0%
! TGAAATATAAGCAAAAAGGT+AGG - chr8_1:20587646-20587665 Msa1184690:intron 25.0%
! TAAAAATTTACACCTAAAGG+AGG - chr8_1:20587801-20587820 Msa1184690:intron 25.0%
! AAAAATTTACACCTAAAGGA+GGG - chr8_1:20587802-20587821 Msa1184690:intron 25.0%
!!! TTTGTTATTTTGTTCTCTAG+TGG - chr8_1:20587341-20587360 Msa1184690:intron 25.0%
!!! CAATCTAAAGATAATGGAAA+AGG - chr8_1:20587464-20587483 Msa1184690:CDS 25.0%
!! TGTCTTTGATTCATCTTTTT+AGG - chr8_1:20587554-20587573 Msa1184690:intron 25.0%
! TGAAATATAAGCAAAAAGGT+AGG - chr8_1:20587646-20587665 Msa1184690:intron 25.0%
! TAAAAATTTACACCTAAAGG+AGG - chr8_1:20587801-20587820 Msa1184690:intron 25.0%
! AAAAATTTACACCTAAAGGA+GGG - chr8_1:20587802-20587821 Msa1184690:intron 25.0%
TTGTAACTAAGGATCACATA+AGG + chr8_1:20587250-20587269 None:intergenic 30.0%
TGAGCTCAATCTAAAGATAA+TGG - chr8_1:20587458-20587477 Msa1184690:CDS 30.0%
TTGCTTATATTTCATTCTGG+AGG + chr8_1:20587640-20587659 None:intergenic 30.0%
ACTTTAACACTTACTTTCTC+CGG + chr8_1:20587741-20587760 None:intergenic 30.0%
GAGAAAGTAAGTGTTAAAGT+TGG - chr8_1:20587741-20587760 Msa1184690:intron 30.0%
GTTGGATTCTCATTGAAAAT+TGG - chr8_1:20587759-20587778 Msa1184690:intron 30.0%
GTTTGACTATGTAACTTCAA+AGG + chr8_1:20587892-20587911 None:intergenic 30.0%
ACAAGATTGTAAGAATTCTC+TGG + chr8_1:20588014-20588033 None:intergenic 30.0%
GAATTCTTACAATCTTGTGA+AGG - chr8_1:20588016-20588035 Msa1184690:CDS 30.0%
AGAATATTGTGGAGAGATTA+AGG - chr8_1:20588043-20588062 Msa1184690:CDS 30.0%
!! TTATATAGCCTTATTACCTC+AGG + chr8_1:20588086-20588105 None:intergenic 30.0%
TTGTAACTAAGGATCACATA+AGG + chr8_1:20587250-20587269 None:intergenic 30.0%
TGAGCTCAATCTAAAGATAA+TGG - chr8_1:20587458-20587477 Msa1184690:CDS 30.0%
TTGCTTATATTTCATTCTGG+AGG + chr8_1:20587640-20587659 None:intergenic 30.0%
ACTTTAACACTTACTTTCTC+CGG + chr8_1:20587741-20587760 None:intergenic 30.0%
GAGAAAGTAAGTGTTAAAGT+TGG - chr8_1:20587741-20587760 Msa1184690:intron 30.0%
GTTGGATTCTCATTGAAAAT+TGG - chr8_1:20587759-20587778 Msa1184690:intron 30.0%
GTTTGACTATGTAACTTCAA+AGG + chr8_1:20587892-20587911 None:intergenic 30.0%
ACAAGATTGTAAGAATTCTC+TGG + chr8_1:20588014-20588033 None:intergenic 30.0%
GAATTCTTACAATCTTGTGA+AGG - chr8_1:20588016-20588035 Msa1184690:CDS 30.0%
AGAATATTGTGGAGAGATTA+AGG - chr8_1:20588043-20588062 Msa1184690:CDS 30.0%
!! TTATATAGCCTTATTACCTC+AGG + chr8_1:20588086-20588105 None:intergenic 30.0%
TGATGAATAATATCAGGTGC+TGG - chr8_1:20587381-20587400 Msa1184690:intron 35.0%
GAAAAGTCTCATTCCATTGA+GGG + chr8_1:20587413-20587432 None:intergenic 35.0%
AGAAAAGTCTCATTCCATTG+AGG + chr8_1:20587414-20587433 None:intergenic 35.0%
AAAATTGAACGAGATGTCAG+TGG + chr8_1:20587920-20587939 None:intergenic 35.0%
TCTTCAAACACTGCTTCTAA+AGG + chr8_1:20587981-20588000 None:intergenic 35.0%
TTTAGAAGCAGTGTTTGAAG+AGG - chr8_1:20587980-20587999 Msa1184690:CDS 35.0%
TTAGAAGCAGTGTTTGAAGA+GGG - chr8_1:20587981-20588000 Msa1184690:CDS 35.0%
GATTGTAAGAATTCTCTGGA+AGG + chr8_1:20588010-20588029 None:intergenic 35.0%
GTGAAGGATGAAGAATATTG+TGG - chr8_1:20588032-20588051 Msa1184690:CDS 35.0%
ATATTGTGGAGAGATTAAGG+TGG - chr8_1:20588046-20588065 Msa1184690:CDS 35.0%
! CTTTTACACCTGAGGTAATA+AGG - chr8_1:20588075-20588094 Msa1184690:CDS 35.0%
TGATCAGAGTTACAATGAAG+AGG - chr8_1:20588195-20588214 Msa1184690:CDS 35.0%
TGATGAATAATATCAGGTGC+TGG - chr8_1:20587381-20587400 Msa1184690:intron 35.0%
GAAAAGTCTCATTCCATTGA+GGG + chr8_1:20587413-20587432 None:intergenic 35.0%
AGAAAAGTCTCATTCCATTG+AGG + chr8_1:20587414-20587433 None:intergenic 35.0%
AAAATTGAACGAGATGTCAG+TGG + chr8_1:20587920-20587939 None:intergenic 35.0%
TCTTCAAACACTGCTTCTAA+AGG + chr8_1:20587981-20588000 None:intergenic 35.0%
TTTAGAAGCAGTGTTTGAAG+AGG - chr8_1:20587980-20587999 Msa1184690:CDS 35.0%
TTAGAAGCAGTGTTTGAAGA+GGG - chr8_1:20587981-20588000 Msa1184690:CDS 35.0%
GATTGTAAGAATTCTCTGGA+AGG + chr8_1:20588010-20588029 None:intergenic 35.0%
GTGAAGGATGAAGAATATTG+TGG - chr8_1:20588032-20588051 Msa1184690:CDS 35.0%
ATATTGTGGAGAGATTAAGG+TGG - chr8_1:20588046-20588065 Msa1184690:CDS 35.0%
! CTTTTACACCTGAGGTAATA+AGG - chr8_1:20588075-20588094 Msa1184690:CDS 35.0%
TGATCAGAGTTACAATGAAG+AGG - chr8_1:20588195-20588214 Msa1184690:CDS 35.0%
GATCCTTAGTTACAAAGCAC+AGG - chr8_1:20587255-20587274 Msa1184690:CDS 40.0%
TCAGAGTTACAATGAAGAGG+AGG - chr8_1:20588198-20588217 Msa1184690:CDS 40.0%
GATCCTTAGTTACAAAGCAC+AGG - chr8_1:20587255-20587274 Msa1184690:CDS 40.0%
TCAGAGTTACAATGAAGAGG+AGG - chr8_1:20588198-20588217 Msa1184690:CDS 40.0%
GCTCCTGTGCTTTGTAACTA+AGG + chr8_1:20587261-20587280 None:intergenic 45.0%
CACAAGAGTTCTGTGCAAGA+AGG - chr8_1:20587280-20587299 Msa1184690:CDS 45.0%
AGTTGCAAAGTCGACGTATG+TGG + chr8_1:20587695-20587714 None:intergenic 45.0%
TTTCCTTTGTGCCCTCCTTT+AGG + chr8_1:20587816-20587835 None:intergenic 45.0%
TACAATGAAGAGGAGGAGAC+TGG - chr8_1:20588205-20588224 Msa1184690:CDS 45.0%
GCTCCTGTGCTTTGTAACTA+AGG + chr8_1:20587261-20587280 None:intergenic 45.0%
CACAAGAGTTCTGTGCAAGA+AGG - chr8_1:20587280-20587299 Msa1184690:CDS 45.0%
AGTTGCAAAGTCGACGTATG+TGG + chr8_1:20587695-20587714 None:intergenic 45.0%
TTTCCTTTGTGCCCTCCTTT+AGG + chr8_1:20587816-20587835 None:intergenic 45.0%
TACAATGAAGAGGAGGAGAC+TGG - chr8_1:20588205-20588224 Msa1184690:CDS 45.0%
GTGCTGGATCTAACCCTCAA+TGG - chr8_1:20587397-20587416 Msa1184690:intron 50.0%
! ACACCTAAAGGAGGGCACAA+AGG - chr8_1:20587810-20587829 Msa1184690:intron 50.0%
!! GGCTCTCACTTTTACACCTG+AGG - chr8_1:20588067-20588086 Msa1184690:CDS 50.0%
AATGAAGAGGAGGAGACTGG+TGG - chr8_1:20588208-20588227 Msa1184690:CDS 50.0%
GTGCTGGATCTAACCCTCAA+TGG - chr8_1:20587397-20587416 Msa1184690:intron 50.0%
! ACACCTAAAGGAGGGCACAA+AGG - chr8_1:20587810-20587829 Msa1184690:intron 50.0%
!! GGCTCTCACTTTTACACCTG+AGG - chr8_1:20588067-20588086 Msa1184690:CDS 50.0%
AATGAAGAGGAGGAGACTGG+TGG - chr8_1:20588208-20588227 Msa1184690:CDS 50.0%
AAGAGGAGGAGACTGGTGGA+TGG - chr8_1:20588212-20588231 Msa1184690:CDS 55.0%
AAGAGGAGGAGACTGGTGGA+TGG - chr8_1:20588212-20588231 Msa1184690:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 20587238 20588255 20587238 ID=Msa1184690;Name=Msa1184690
chr8_1 mRNA 20587238 20588255 20587238 ID=Msa1184690-mRNA-1;Parent=Msa1184690;Name=Msa1184690-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|0.66|0.25|4|0|114
chr8_1 exon 20588192 20588255 20588192 ID=Msa1184690-mRNA-1:exon:7920;Parent=Msa1184690-mRNA-1
chr8_1 exon 20587970 20588096 20587970 ID=Msa1184690-mRNA-1:exon:7919;Parent=Msa1184690-mRNA-1
chr8_1 exon 20587405 20587486 20587405 ID=Msa1184690-mRNA-1:exon:7918;Parent=Msa1184690-mRNA-1
chr8_1 exon 20587238 20587306 20587238 ID=Msa1184690-mRNA-1:exon:7917;Parent=Msa1184690-mRNA-1
chr8_1 CDS 20588192 20588255 20588192 ID=Msa1184690-mRNA-1:cds;Parent=Msa1184690-mRNA-1
chr8_1 CDS 20587970 20588096 20587970 ID=Msa1184690-mRNA-1:cds;Parent=Msa1184690-mRNA-1
chr8_1 CDS 20587405 20587486 20587405 ID=Msa1184690-mRNA-1:cds;Parent=Msa1184690-mRNA-1
chr8_1 CDS 20587238 20587306 20587238 ID=Msa1184690-mRNA-1:cds;Parent=Msa1184690-mRNA-1
Gene Sequence

>Msa1184690

AGCATAGATCCTTATGTGATCCTTAGTTACAAAGCACAGGAGCACAAGAGTTCTGTGCAAGAAGGTGCTGGATCTAACCCTCAATGGAATGAGACTTTTCTTTTCACTGTTTCTGACACTGCTTCTGAGCTCAATCTAAAGATAATGGAAAAGGACAACTATAATAATGATGATAATATTGGAGAAGCAATCCTTCCAGAGAATTCTTACAATCTTGTGAAGGATGAAGAATATTGTGGAGAGATTAAGGTGGCTCTCACTTTTACACCTGAGAGAAATGATGATCAGAGTTACAATGAAGAGGAGGAGACTGGTGGATGGAAACAATCAGCTAGTGACTTT

Protein sequence

>Msa1184690

SIDPYVILSYKAQEHKSSVQEGAGSNPQWNETFLFTVSDTASELNLKIMEKDNYNNDDNIGEAILPENSYNLVKDEEYCGEIKVALTFTPERNDDQSYNEEEETGGWKQSASDF