AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0604350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604350 MtrunA17_Chr3g0131781 79.861 144 7 2 1 122 1 144 2.60e-68 206
Msa0604350 MtrunA17_Chr8g0385941 42.442 172 42 9 1 121 1 166 1.05e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTCGATCTTGGTCTTT+TGG 0.187350 4_2:-49889278 None:intergenic
TGGTAGCTGGAAAGTGAAAA+AGG 0.224871 4_2:+49889252 Msa0604350:CDS
TCGTTGCTCCGCCGTTTATT+TGG 0.233205 4_2:-49888987 None:intergenic
AACACCGACGGTGTAATTAT+TGG 0.331686 4_2:+49889232 Msa0604350:CDS
CAAAAGACCAAGATCGAATT+GGG 0.359194 4_2:+49889279 Msa0604350:CDS
CCAAAAGACCAAGATCGAAT+TGG 0.364115 4_2:+49889278 Msa0604350:CDS
AAATCATGGCGATTCGGCTT+CGG 0.384660 4_2:+49889126 Msa0604350:CDS
GGTCTCCGATGTCGCCTAAT+CGG 0.413080 4_2:-49889071 None:intergenic
CCGCCGTTTATTTGGACCTC+CGG 0.440203 4_2:-49888979 None:intergenic
TTCGGCTTCGGCAACGTCTC+AGG 0.441191 4_2:+49889138 Msa0604350:CDS
CCACCGGAGGTCCAAATAAA+CGG 0.460030 4_2:+49888976 Msa0604350:CDS
GAAGAAACCCAATTCGATCT+TGG 0.472216 4_2:-49889286 None:intergenic
TTCGATCCGTGAGGTTCTGA+AGG 0.475990 4_2:-49889027 None:intergenic
AGTTCAAAATCATGGCGATT+CGG 0.480998 4_2:+49889120 Msa0604350:CDS
CGCCTAATCGGACGCTGGGT+CGG 0.497118 4_2:-49889059 None:intergenic
GCGGCGAGGGTGGGAATCAG+AGG 0.498456 4_2:-49889097 None:intergenic
CTCGCCGCAGTTCAAAATCA+TGG 0.500043 4_2:+49889112 Msa0604350:CDS
AATAATTACACCGTCGGTGT+TGG 0.505458 4_2:-49889230 None:intergenic
TTTATTTGGACCTCCGGTGG+CGG 0.518429 4_2:-49888973 None:intergenic
GTTTCTTCGCGAATTGAAGA+AGG 0.522515 4_2:+49889301 Msa0604350:CDS
GATGTCGCCTAATCGGACGC+TGG 0.529379 4_2:-49889064 None:intergenic
ACGGTGTAATTATTGGTAGC+TGG 0.534282 4_2:+49889239 Msa0604350:CDS
ATGTCGCCTAATCGGACGCT+GGG 0.537206 4_2:-49889063 None:intergenic
AATGGAAGAGGTTCCGCCAC+CGG 0.544579 4_2:+49888960 None:intergenic
TCGATCCGTGAGGTTCTGAA+GGG 0.559702 4_2:-49889026 None:intergenic
CACCGACCCAGCGTCCGATT+AGG 0.565703 4_2:+49889057 Msa0604350:CDS
CCGTTTATTTGGACCTCCGG+TGG 0.575464 4_2:-49888976 None:intergenic
CGGCGAGGGTGGGAATCAGA+GGG 0.591797 4_2:-49889096 None:intergenic
GAGGGTGGGAATCAGAGGGT+TGG 0.602586 4_2:-49889092 None:intergenic
GAAACGGAAGAAAATGGAAG+AGG 0.606203 4_2:+49888948 None:intergenic
GGAAGAGGTTCCGCCACCGG+AGG 0.617458 4_2:+49888963 Msa0604350:CDS
CGATCCGTGAGGTTCTGAAG+GGG 0.626502 4_2:-49889025 None:intergenic
AGCGTCCGATTAGGCGACAT+CGG 0.639876 4_2:+49889066 Msa0604350:CDS
GATCCGTGAGGTTCTGAAGG+GGG 0.654909 4_2:-49889024 None:intergenic
GCTACCAATAATTACACCGT+CGG 0.667969 4_2:-49889236 None:intergenic
GACGTAGAAACCAACACCGA+CGG 0.675808 4_2:+49889220 Msa0604350:intron
CAGAACCTCACGGATCGAAG+CGG 0.686667 4_2:+49889031 Msa0604350:CDS
TGTTGCCGCTTCGATCCGTG+AGG 0.714492 4_2:-49889036 None:intergenic
CCGGAGGTCCAAATAAACGG+CGG 0.738172 4_2:+49888979 Msa0604350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTGAAGGTGATAATT+CGG + chr4_2:49889357-49889376 Msa0604350:CDS 25.0%
! AACCAGTTCAAAAAAAAATC+TGG + chr4_2:49889389-49889408 Msa0604350:CDS 25.0%
!!! AACCAGATTTTTTTTTGAAC+TGG - chr4_2:49889394-49889413 None:intergenic 25.0%
! CTGGTTAAAAATAAGTAAAC+CGG + chr4_2:49889408-49889427 Msa0604350:CDS 25.0%
! TCCTTTGACTAATTTGACTT+CGG + chr4_2:49889179-49889198 Msa0604350:intron 30.0%
! GATTTGTATCGAGATTTTGA+AGG + chr4_2:49889346-49889365 Msa0604350:CDS 30.0%
!! GATTTTGAAGGTGATAATTC+GGG + chr4_2:49889358-49889377 Msa0604350:CDS 30.0%
AGTTCAAAATCATGGCGATT+CGG + chr4_2:49889120-49889139 Msa0604350:CDS 35.0%
ACCGAAGTCAAATTAGTCAA+AGG - chr4_2:49889183-49889202 None:intergenic 35.0%
CAAAAGACCAAGATCGAATT+GGG + chr4_2:49889279-49889298 Msa0604350:CDS 35.0%
! ATCGCCATGATTTTGAACTG+CGG - chr4_2:49889119-49889138 None:intergenic 40.0%
TCTACGTCTTCCATCGAAAT+CGG - chr4_2:49889208-49889227 None:intergenic 40.0%
AATAATTACACCGTCGGTGT+TGG - chr4_2:49889233-49889252 None:intergenic 40.0%
AACACCGACGGTGTAATTAT+TGG + chr4_2:49889232-49889251 Msa0604350:CDS 40.0%
GCTACCAATAATTACACCGT+CGG - chr4_2:49889239-49889258 None:intergenic 40.0%
! ACGGTGTAATTATTGGTAGC+TGG + chr4_2:49889239-49889258 Msa0604350:CDS 40.0%
TGGTAGCTGGAAAGTGAAAA+AGG + chr4_2:49889252-49889271 Msa0604350:CDS 40.0%
GGTAGCTGGAAAGTGAAAAA+GGG + chr4_2:49889253-49889272 Msa0604350:CDS 40.0%
!! CCAATTCGATCTTGGTCTTT+TGG - chr4_2:49889281-49889300 None:intergenic 40.0%
CCAAAAGACCAAGATCGAAT+TGG + chr4_2:49889278-49889297 Msa0604350:CDS 40.0%
GAAGAAACCCAATTCGATCT+TGG - chr4_2:49889289-49889308 None:intergenic 40.0%
GTTTCTTCGCGAATTGAAGA+AGG + chr4_2:49889301-49889320 Msa0604350:CDS 40.0%
! ATGATTTTGAACTGCGGCGA+GGG - chr4_2:49889113-49889132 None:intergenic 45.0%
! AAATCATGGCGATTCGGCTT+CGG + chr4_2:49889126-49889145 Msa0604350:CDS 45.0%
ACTTCGGTTTCCGATTTCGA+TGG + chr4_2:49889195-49889214 Msa0604350:intron 45.0%
TTTATTTGGACCTCCGGTGG+CGG - chr4_2:49888976-49888995 None:intergenic 50.0%
CCACCGGAGGTCCAAATAAA+CGG + chr4_2:49888976-49888995 Msa0604350:CDS 50.0%
TCGTTGCTCCGCCGTTTATT+TGG - chr4_2:49888990-49889009 None:intergenic 50.0%
!! TCGATCCGTGAGGTTCTGAA+GGG - chr4_2:49889029-49889048 None:intergenic 50.0%
! TTCGATCCGTGAGGTTCTGA+AGG - chr4_2:49889030-49889049 None:intergenic 50.0%
! CATGATTTTGAACTGCGGCG+AGG - chr4_2:49889114-49889133 None:intergenic 50.0%
CTCGCCGCAGTTCAAAATCA+TGG + chr4_2:49889112-49889131 Msa0604350:CDS 50.0%
GTCAAAGGACGAGTCTTCGA+GGG - chr4_2:49889168-49889187 None:intergenic 50.0%
AGTCAAAGGACGAGTCTTCG+AGG - chr4_2:49889169-49889188 None:intergenic 50.0%
GACGTAGAAACCAACACCGA+CGG + chr4_2:49889220-49889239 Msa0604350:intron 50.0%
CCGTTTATTTGGACCTCCGG+TGG - chr4_2:49888979-49888998 None:intergenic 55.0%
CCGCCGTTTATTTGGACCTC+CGG - chr4_2:49888982-49889001 None:intergenic 55.0%
CCGGAGGTCCAAATAAACGG+CGG + chr4_2:49888979-49888998 Msa0604350:CDS 55.0%
CTACCCCCTTCAGAACCTCA+CGG + chr4_2:49889021-49889040 Msa0604350:CDS 55.0%
!! GATCCGTGAGGTTCTGAAGG+GGG - chr4_2:49889027-49889046 None:intergenic 55.0%
!! CGATCCGTGAGGTTCTGAAG+GGG - chr4_2:49889028-49889047 None:intergenic 55.0%
CAGAACCTCACGGATCGAAG+CGG + chr4_2:49889031-49889050 Msa0604350:CDS 55.0%
ATGTCGCCTAATCGGACGCT+GGG - chr4_2:49889066-49889085 None:intergenic 55.0%
AGCGTCCGATTAGGCGACAT+CGG + chr4_2:49889066-49889085 Msa0604350:CDS 55.0%
GGTCTCCGATGTCGCCTAAT+CGG - chr4_2:49889074-49889093 None:intergenic 55.0%
! TTTTGAACTGCGGCGAGGGT+GGG - chr4_2:49889109-49889128 None:intergenic 55.0%
! ATTTTGAACTGCGGCGAGGG+TGG - chr4_2:49889110-49889129 None:intergenic 55.0%
! TGTTGCCGCTTCGATCCGTG+AGG - chr4_2:49889039-49889058 None:intergenic 60.0%
GATGTCGCCTAATCGGACGC+TGG - chr4_2:49889067-49889086 None:intergenic 60.0%
GAGGGTGGGAATCAGAGGGT+TGG - chr4_2:49889095-49889114 None:intergenic 60.0%
TTCGGCTTCGGCAACGTCTC+AGG + chr4_2:49889138-49889157 Msa0604350:CDS 60.0%
CACCGACCCAGCGTCCGATT+AGG + chr4_2:49889057-49889076 Msa0604350:CDS 65.0%
CGCCTAATCGGACGCTGGGT+CGG - chr4_2:49889062-49889081 None:intergenic 65.0%
CGGCGAGGGTGGGAATCAGA+GGG - chr4_2:49889099-49889118 None:intergenic 65.0%
GGAAGAGGTTCCGCCACCGG+AGG + chr4_2:49888963-49888982 Msa0604350:CDS 70.0%
GCGGCGAGGGTGGGAATCAG+AGG - chr4_2:49889100-49889119 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 49888961 49889435 49888961 ID=Msa0604350;Name=Msa0604350
chr4_2 mRNA 49888961 49889435 49888961 ID=Msa0604350-mRNA-1;Parent=Msa0604350;Name=Msa0604350-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|2|0|137
chr4_2 exon 49888961 49889159 49888961 ID=Msa0604350-mRNA-1:exon:11566;Parent=Msa0604350-mRNA-1
chr4_2 exon 49889221 49889435 49889221 ID=Msa0604350-mRNA-1:exon:11567;Parent=Msa0604350-mRNA-1
chr4_2 CDS 49888961 49889159 49888961 ID=Msa0604350-mRNA-1:cds;Parent=Msa0604350-mRNA-1
chr4_2 CDS 49889221 49889435 49889221 ID=Msa0604350-mRNA-1:cds;Parent=Msa0604350-mRNA-1
Gene Sequence

>Msa0604350

ATGGAAGAGGTTCCGCCACCGGAGGTCCAAATAAACGGCGGAGCAACGACTGCAGCAACACTACCCCCTTCAGAACCTCACGGATCGAAGCGGCAACACCGACCCAGCGTCCGATTAGGCGACATCGGAGACCAACCCTCTGATTCCCACCCTCGCCGCAGTTCAAAATCATGGCGATTCGGCTTCGGCAACGTCTCAGACGTAGAAACCAACACCGACGGTGTAATTATTGGTAGCTGGAAAGTGAAAAAGGGTTCCAAAAGACCAAGATCGAATTGGGTTTCTTCGCGAATTGAAGAAGGTGAAGATGTTAATAATGATGATGATTTGTATCGAGATTTTGAAGGTGATAATTCGGGTAGTAGAAAACCAGTTCAAAAAAAAATCTGGTTAAAAATAAGTAAACCGGTTTAA

Protein sequence

>Msa0604350

MEEVPPPEVQINGGATTAATLPPSEPHGSKRQHRPSVRLGDIGDQPSDSHPRRSSKSWRFGFGNVSDVETNTDGVIIGSWKVKKGSKRPRSNWVSSRIEEGEDVNNDDDLYRDFEGDNSGSRKPVQKKIWLKISKPV*