AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0636170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0636170 MtrunA17_Chr3g0131781 79.861 144 7 2 1 122 1 144 2.60e-68 206
Msa0636170 MtrunA17_Chr8g0385941 42.442 172 42 9 1 121 1 166 1.05e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTCGATCTTGGTCTTT+TGG 0.187350 4_3:-26030818 None:intergenic
TGGTAGCTGGAAAGTGAAAA+AGG 0.224871 4_3:+26030792 Msa0636170:CDS
TCGTTGCTCCGCCGTTTATT+TGG 0.233205 4_3:-26030527 None:intergenic
AACACCGACGGTGTAATTAT+TGG 0.331686 4_3:+26030772 Msa0636170:CDS
CAAAAGACCAAGATCGAATT+GGG 0.359194 4_3:+26030819 Msa0636170:CDS
CCAAAAGACCAAGATCGAAT+TGG 0.364115 4_3:+26030818 Msa0636170:CDS
AAATCATGGCGATTCGGCTT+CGG 0.384660 4_3:+26030666 Msa0636170:CDS
GGTCTCCGATGTCGCCTAAT+CGG 0.413080 4_3:-26030611 None:intergenic
CCGCCGTTTATTTGGACCTC+CGG 0.440203 4_3:-26030519 None:intergenic
TTCGGCTTCGGCAACGTCTC+AGG 0.441191 4_3:+26030678 Msa0636170:CDS
CCACCGGAGGTCCAAATAAA+CGG 0.460030 4_3:+26030516 Msa0636170:CDS
GAAGAAACCCAATTCGATCT+TGG 0.472216 4_3:-26030826 None:intergenic
TTCGATCCGTGAGGTTCTGA+AGG 0.475990 4_3:-26030567 None:intergenic
AGTTCAAAATCATGGCGATT+CGG 0.480998 4_3:+26030660 Msa0636170:CDS
CGCCTAATCGGACGCTGGGT+CGG 0.497118 4_3:-26030599 None:intergenic
GCGGCGAGGGTGGGAATCAG+AGG 0.498456 4_3:-26030637 None:intergenic
CTCGCCGCAGTTCAAAATCA+TGG 0.500043 4_3:+26030652 Msa0636170:CDS
AATAATTACACCGTCGGTGT+TGG 0.505458 4_3:-26030770 None:intergenic
TTTATTTGGACCTCCGGTGG+CGG 0.518429 4_3:-26030513 None:intergenic
GTTTCTTCGCGAATTGAAGA+AGG 0.522515 4_3:+26030841 Msa0636170:CDS
GATGTCGCCTAATCGGACGC+TGG 0.529379 4_3:-26030604 None:intergenic
ACGGTGTAATTATTGGTAGC+TGG 0.534282 4_3:+26030779 Msa0636170:CDS
ATGTCGCCTAATCGGACGCT+GGG 0.537206 4_3:-26030603 None:intergenic
AATGGAAGAGGTTCCGCCAC+CGG 0.544579 4_3:+26030500 None:intergenic
TCGATCCGTGAGGTTCTGAA+GGG 0.559702 4_3:-26030566 None:intergenic
CACCGACCCAGCGTCCGATT+AGG 0.565703 4_3:+26030597 Msa0636170:CDS
CCGTTTATTTGGACCTCCGG+TGG 0.575464 4_3:-26030516 None:intergenic
CGGCGAGGGTGGGAATCAGA+GGG 0.591797 4_3:-26030636 None:intergenic
GAGGGTGGGAATCAGAGGGT+TGG 0.602586 4_3:-26030632 None:intergenic
GAAACGGAAGAAAATGGAAG+AGG 0.606203 4_3:+26030488 None:intergenic
GGAAGAGGTTCCGCCACCGG+AGG 0.617458 4_3:+26030503 Msa0636170:CDS
CGATCCGTGAGGTTCTGAAG+GGG 0.626502 4_3:-26030565 None:intergenic
AGCGTCCGATTAGGCGACAT+CGG 0.639876 4_3:+26030606 Msa0636170:CDS
GATCCGTGAGGTTCTGAAGG+GGG 0.654909 4_3:-26030564 None:intergenic
GCTACCAATAATTACACCGT+CGG 0.667969 4_3:-26030776 None:intergenic
GACGTAGAAACCAACACCGA+CGG 0.675808 4_3:+26030760 Msa0636170:intron
CAGAACCTCACGGATCGAAG+CGG 0.686667 4_3:+26030571 Msa0636170:CDS
TGTTGCCGCTTCGATCCGTG+AGG 0.714492 4_3:-26030576 None:intergenic
CCGGAGGTCCAAATAAACGG+CGG 0.738172 4_3:+26030519 Msa0636170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTGAAGGTGATAATT+CGG + chr4_3:26030897-26030916 Msa0636170:CDS 25.0%
! AACCAGTTCAAAAAAAAATC+TGG + chr4_3:26030929-26030948 Msa0636170:CDS 25.0%
!!! AACCAGATTTTTTTTTGAAC+TGG - chr4_3:26030934-26030953 None:intergenic 25.0%
! CTGGTTAAAAATAAGTAAAC+CGG + chr4_3:26030948-26030967 Msa0636170:CDS 25.0%
! TCCTTTGACTAATTTGACTT+CGG + chr4_3:26030719-26030738 Msa0636170:intron 30.0%
! GATTTGTATCGAGATTTTGA+AGG + chr4_3:26030886-26030905 Msa0636170:CDS 30.0%
!! GATTTTGAAGGTGATAATTC+GGG + chr4_3:26030898-26030917 Msa0636170:CDS 30.0%
AGTTCAAAATCATGGCGATT+CGG + chr4_3:26030660-26030679 Msa0636170:CDS 35.0%
ACCGAAGTCAAATTAGTCAA+AGG - chr4_3:26030723-26030742 None:intergenic 35.0%
CAAAAGACCAAGATCGAATT+GGG + chr4_3:26030819-26030838 Msa0636170:CDS 35.0%
! ATCGCCATGATTTTGAACTG+CGG - chr4_3:26030659-26030678 None:intergenic 40.0%
TCTACGTCTTCCATCGAAAT+CGG - chr4_3:26030748-26030767 None:intergenic 40.0%
AATAATTACACCGTCGGTGT+TGG - chr4_3:26030773-26030792 None:intergenic 40.0%
AACACCGACGGTGTAATTAT+TGG + chr4_3:26030772-26030791 Msa0636170:CDS 40.0%
GCTACCAATAATTACACCGT+CGG - chr4_3:26030779-26030798 None:intergenic 40.0%
! ACGGTGTAATTATTGGTAGC+TGG + chr4_3:26030779-26030798 Msa0636170:CDS 40.0%
TGGTAGCTGGAAAGTGAAAA+AGG + chr4_3:26030792-26030811 Msa0636170:CDS 40.0%
GGTAGCTGGAAAGTGAAAAA+GGG + chr4_3:26030793-26030812 Msa0636170:CDS 40.0%
!! CCAATTCGATCTTGGTCTTT+TGG - chr4_3:26030821-26030840 None:intergenic 40.0%
CCAAAAGACCAAGATCGAAT+TGG + chr4_3:26030818-26030837 Msa0636170:CDS 40.0%
GAAGAAACCCAATTCGATCT+TGG - chr4_3:26030829-26030848 None:intergenic 40.0%
GTTTCTTCGCGAATTGAAGA+AGG + chr4_3:26030841-26030860 Msa0636170:CDS 40.0%
! ATGATTTTGAACTGCGGCGA+GGG - chr4_3:26030653-26030672 None:intergenic 45.0%
! AAATCATGGCGATTCGGCTT+CGG + chr4_3:26030666-26030685 Msa0636170:CDS 45.0%
ACTTCGGTTTCCGATTTCGA+TGG + chr4_3:26030735-26030754 Msa0636170:intron 45.0%
TTTATTTGGACCTCCGGTGG+CGG - chr4_3:26030516-26030535 None:intergenic 50.0%
CCACCGGAGGTCCAAATAAA+CGG + chr4_3:26030516-26030535 Msa0636170:CDS 50.0%
TCGTTGCTCCGCCGTTTATT+TGG - chr4_3:26030530-26030549 None:intergenic 50.0%
!! TCGATCCGTGAGGTTCTGAA+GGG - chr4_3:26030569-26030588 None:intergenic 50.0%
! TTCGATCCGTGAGGTTCTGA+AGG - chr4_3:26030570-26030589 None:intergenic 50.0%
! CATGATTTTGAACTGCGGCG+AGG - chr4_3:26030654-26030673 None:intergenic 50.0%
CTCGCCGCAGTTCAAAATCA+TGG + chr4_3:26030652-26030671 Msa0636170:CDS 50.0%
GTCAAAGGACGAGTCTTCGA+GGG - chr4_3:26030708-26030727 None:intergenic 50.0%
AGTCAAAGGACGAGTCTTCG+AGG - chr4_3:26030709-26030728 None:intergenic 50.0%
GACGTAGAAACCAACACCGA+CGG + chr4_3:26030760-26030779 Msa0636170:intron 50.0%
CCGTTTATTTGGACCTCCGG+TGG - chr4_3:26030519-26030538 None:intergenic 55.0%
CCGCCGTTTATTTGGACCTC+CGG - chr4_3:26030522-26030541 None:intergenic 55.0%
CCGGAGGTCCAAATAAACGG+CGG + chr4_3:26030519-26030538 Msa0636170:CDS 55.0%
CTACCCCCTTCAGAACCTCA+CGG + chr4_3:26030561-26030580 Msa0636170:CDS 55.0%
!! GATCCGTGAGGTTCTGAAGG+GGG - chr4_3:26030567-26030586 None:intergenic 55.0%
!! CGATCCGTGAGGTTCTGAAG+GGG - chr4_3:26030568-26030587 None:intergenic 55.0%
CAGAACCTCACGGATCGAAG+CGG + chr4_3:26030571-26030590 Msa0636170:CDS 55.0%
ATGTCGCCTAATCGGACGCT+GGG - chr4_3:26030606-26030625 None:intergenic 55.0%
AGCGTCCGATTAGGCGACAT+CGG + chr4_3:26030606-26030625 Msa0636170:CDS 55.0%
GGTCTCCGATGTCGCCTAAT+CGG - chr4_3:26030614-26030633 None:intergenic 55.0%
! TTTTGAACTGCGGCGAGGGT+GGG - chr4_3:26030649-26030668 None:intergenic 55.0%
! ATTTTGAACTGCGGCGAGGG+TGG - chr4_3:26030650-26030669 None:intergenic 55.0%
! TGTTGCCGCTTCGATCCGTG+AGG - chr4_3:26030579-26030598 None:intergenic 60.0%
GATGTCGCCTAATCGGACGC+TGG - chr4_3:26030607-26030626 None:intergenic 60.0%
GAGGGTGGGAATCAGAGGGT+TGG - chr4_3:26030635-26030654 None:intergenic 60.0%
TTCGGCTTCGGCAACGTCTC+AGG + chr4_3:26030678-26030697 Msa0636170:CDS 60.0%
CACCGACCCAGCGTCCGATT+AGG + chr4_3:26030597-26030616 Msa0636170:CDS 65.0%
CGCCTAATCGGACGCTGGGT+CGG - chr4_3:26030602-26030621 None:intergenic 65.0%
CGGCGAGGGTGGGAATCAGA+GGG - chr4_3:26030639-26030658 None:intergenic 65.0%
GGAAGAGGTTCCGCCACCGG+AGG + chr4_3:26030503-26030522 Msa0636170:CDS 70.0%
GCGGCGAGGGTGGGAATCAG+AGG - chr4_3:26030640-26030659 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 26030501 26030975 26030501 ID=Msa0636170;Name=Msa0636170
chr4_3 mRNA 26030501 26030975 26030501 ID=Msa0636170-mRNA-1;Parent=Msa0636170;Name=Msa0636170-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|2|0|137
chr4_3 exon 26030501 26030699 26030501 ID=Msa0636170-mRNA-1:exon:6557;Parent=Msa0636170-mRNA-1
chr4_3 exon 26030761 26030975 26030761 ID=Msa0636170-mRNA-1:exon:6558;Parent=Msa0636170-mRNA-1
chr4_3 CDS 26030501 26030699 26030501 ID=Msa0636170-mRNA-1:cds;Parent=Msa0636170-mRNA-1
chr4_3 CDS 26030761 26030975 26030761 ID=Msa0636170-mRNA-1:cds;Parent=Msa0636170-mRNA-1
Gene Sequence

>Msa0636170

ATGGAAGAGGTTCCGCCACCGGAGGTCCAAATAAACGGCGGAGCAACGACTGCAGCAACACTACCCCCTTCAGAACCTCACGGATCGAAGCGGCAACACCGACCCAGCGTCCGATTAGGCGACATCGGAGACCAACCCTCTGATTCCCACCCTCGCCGCAGTTCAAAATCATGGCGATTCGGCTTCGGCAACGTCTCAGACGTAGAAACCAACACCGACGGTGTAATTATTGGTAGCTGGAAAGTGAAAAAGGGTTCCAAAAGACCAAGATCGAATTGGGTTTCTTCGCGAATTGAAGAAGGTGAAGATGTTAATAATGATGATGATTTGTATCGAGATTTTGAAGGTGATAATTCGGGTAGTAGAAAACCAGTTCAAAAAAAAATCTGGTTAAAAATAAGTAAACCGGTTTAA

Protein sequence

>Msa0636170

MEEVPPPEVQINGGATTAATLPPSEPHGSKRQHRPSVRLGDIGDQPSDSHPRRSSKSWRFGFGNVSDVETNTDGVIIGSWKVKKGSKRPRSNWVSSRIEEGEDVNNDDDLYRDFEGDNSGSRKPVQKKIWLKISKPV*