AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0016570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0016570 MtrunA17_Chr7g0255021 35.106 94 54 2 14 107 75 161 1.51e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCCAACTTCTTCATTA+AGG 0.234873 1_1:-27493530 None:intergenic
TTCACAAGTGATGTATGATA+TGG 0.297312 1_1:-27493555 None:intergenic
AGGAAACTAATGACACTAAA+AGG 0.309439 1_1:+27493741 Msa0016570:CDS
ATGAGAATTTCATCCTCCTT+TGG 0.374944 1_1:-27493600 None:intergenic
CTCTGATCAATCCCAACAAT+TGG 0.395664 1_1:-27493651 None:intergenic
ATTTCAAAGGATCCAATTGT+TGG 0.406190 1_1:+27493639 Msa0016570:CDS
ATTAACCTTAATGAAGAAGT+TGG 0.447537 1_1:+27493525 Msa0016570:CDS
TTTCAAAGGATCCAATTGTT+GGG 0.456962 1_1:+27493640 Msa0016570:CDS
ATGAAATTCTCATTCAATCA+TGG 0.497742 1_1:+27493610 Msa0016570:CDS
ACACCACAAGTTTGCACTCA+AGG 0.503579 1_1:+27493489 Msa0016570:CDS
AGTTTGCACTCAAGGTGGCT+TGG 0.506608 1_1:+27493497 Msa0016570:CDS
TCACAAGTGATGTATGATAT+GGG 0.522775 1_1:-27493554 None:intergenic
ATCATGGCTCAATATTTCAA+AGG 0.547059 1_1:+27493626 Msa0016570:CDS
GAAAGTAAAATTTCAGCCAA+AGG 0.574785 1_1:+27493584 Msa0016570:CDS
TGGGTTTGAATGGGTAGTAG+TGG 0.601769 1_1:+27493438 None:intergenic
AGTAAAATTTCAGCCAAAGG+AGG 0.612051 1_1:+27493587 Msa0016570:CDS
CCACAAGTTTGCACTCAAGG+TGG 0.655877 1_1:+27493492 Msa0016570:CDS
CCACCTTGAGTGCAAACTTG+TGG 0.671803 1_1:-27493492 None:intergenic
ATTGTTGGGATTGATCAGAG+AGG 0.736327 1_1:+27493654 Msa0016570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAACCTTAATGAAGAAGT+TGG + chr1_1:27493525-27493544 Msa0016570:CDS 25.0%
! AATTTCCAACTTCTTCATTA+AGG - chr1_1:27493533-27493552 None:intergenic 25.0%
! ATGAAATTCTCATTCAATCA+TGG + chr1_1:27493610-27493629 Msa0016570:CDS 25.0%
TCACAAGTGATGTATGATAT+GGG - chr1_1:27493557-27493576 None:intergenic 30.0%
TTCACAAGTGATGTATGATA+TGG - chr1_1:27493558-27493577 None:intergenic 30.0%
GAAAGTAAAATTTCAGCCAA+AGG + chr1_1:27493584-27493603 Msa0016570:CDS 30.0%
ATCATGGCTCAATATTTCAA+AGG + chr1_1:27493626-27493645 Msa0016570:CDS 30.0%
ATTTCAAAGGATCCAATTGT+TGG + chr1_1:27493639-27493658 Msa0016570:CDS 30.0%
TTTCAAAGGATCCAATTGTT+GGG + chr1_1:27493640-27493659 Msa0016570:CDS 30.0%
AGGAAACTAATGACACTAAA+AGG + chr1_1:27493741-27493760 Msa0016570:CDS 30.0%
AGTAAAATTTCAGCCAAAGG+AGG + chr1_1:27493587-27493606 Msa0016570:CDS 35.0%
ATGAGAATTTCATCCTCCTT+TGG - chr1_1:27493603-27493622 None:intergenic 35.0%
! ATCAGAGAGGAGATAGTTTT+TGG + chr1_1:27493667-27493686 Msa0016570:CDS 35.0%
CTCTGATCAATCCCAACAAT+TGG - chr1_1:27493654-27493673 None:intergenic 40.0%
ATTGTTGGGATTGATCAGAG+AGG + chr1_1:27493654-27493673 Msa0016570:CDS 40.0%
!! GAGGAGATAGTTTTTGGAAG+AGG + chr1_1:27493673-27493692 Msa0016570:CDS 40.0%
!! GCGACAAGAATTTTCAAGAG+AGG + chr1_1:27493721-27493740 Msa0016570:CDS 40.0%
ACACCACAAGTTTGCACTCA+AGG + chr1_1:27493489-27493508 Msa0016570:CDS 45.0%
CCACCTTGAGTGCAAACTTG+TGG - chr1_1:27493495-27493514 None:intergenic 50.0%
CCACAAGTTTGCACTCAAGG+TGG + chr1_1:27493492-27493511 Msa0016570:CDS 50.0%
AGTTTGCACTCAAGGTGGCT+TGG + chr1_1:27493497-27493516 Msa0016570:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 27493447 27493770 27493447 ID=Msa0016570;Name=Msa0016570
chr1_1 mRNA 27493447 27493770 27493447 ID=Msa0016570-mRNA-1;Parent=Msa0016570;Name=Msa0016570-mRNA-1;_AED=0.36;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|107
chr1_1 exon 27493447 27493770 27493447 ID=Msa0016570-mRNA-1:exon:9275;Parent=Msa0016570-mRNA-1
chr1_1 CDS 27493447 27493770 27493447 ID=Msa0016570-mRNA-1:cds;Parent=Msa0016570-mRNA-1
Gene Sequence

>Msa0016570

ATGGGTAGTAGTGGTGTGCAATTAAATGATCAAGAGCTTGAAACACCACAAGTTTGCACTCAAGGTGGCTTGGAAACTATTAACCTTAATGAAGAAGTTGGAAATTACCCATATCATACATCACTTGTGAATACTTCGAAAGTAAAATTTCAGCCAAAGGAGGATGAAATTCTCATTCAATCATGGCTCAATATTTCAAAGGATCCAATTGTTGGGATTGATCAGAGAGGAGATAGTTTTTGGAAGAGGATTGATGAAGTTTATAACAACTACAGCGACAAGAATTTTCAAGAGAGGAAACTAATGACACTAAAAGGTCGATGA

Protein sequence

>Msa0016570

MGSSGVQLNDQELETPQVCTQGGLETINLNEEVGNYPYHTSLVNTSKVKFQPKEDEILIQSWLNISKDPIVGIDQRGDSFWKRIDEVYNNYSDKNFQERKLMTLKGR*