AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0693430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0693430 MtrunA17_Chr4g0043821 94.570 221 10 1 1 219 1 221 2.05e-154 427
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0693430 AT1G30070.1 66.516 221 70 1 1 217 1 221 1.42e-107 308
Msa0693430 AT1G30070.2 64.474 228 70 2 1 217 1 228 1.14e-104 301

Find 72 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTGACATCAAACGAATT+TGG 0.215867 4_4:+62336773 None:intergenic
TAAACTTAAACACTTATTCT+TGG 0.241165 4_4:-62335796 Msa0693430:three_prime_UTR
GCTCAACGATAGCTACTTAA+AGG 0.259882 4_4:+62336198 None:intergenic
ACCAGAGAAATGTAAGATTT+TGG 0.261703 4_4:-62336690 Msa0693430:CDS
ATATGCTTCACTTAATTTCA+TGG 0.296866 4_4:-62336131 Msa0693430:three_prime_UTR
ACCAAAATCTTACATTTCTC+TGG 0.339359 4_4:+62336689 None:intergenic
CTCCTCTCGCTTCATTCAGT+TGG 0.363708 4_4:-62337622 Msa0693430:CDS
ACCGTAGTTCCGGTTGAAAT+TGG 0.369259 4_4:+62337671 None:intergenic
CGCTTCATTCAGTTGGGATC+AGG 0.369919 4_4:-62337615 Msa0693430:CDS
CAAATCTAGCCAGTTTGCTT+TGG 0.378797 4_4:+62336628 None:intergenic
TCTTTCTCTTTATCCATGGC+TGG 0.378975 4_4:+62336496 None:intergenic
ACATATATACACCGACAAAA+AGG 0.384211 4_4:-62337973 Msa0693430:five_prime_UTR
TCCTCTCGCTTCATTCAGTT+GGG 0.385459 4_4:-62337621 Msa0693430:CDS
CCAATCCATCTCTTTGTTGT+GGG 0.393164 4_4:-62335974 Msa0693430:three_prime_UTR
AGAGGAATTCGCTTTAGATT+TGG 0.395316 4_4:-62337907 Msa0693430:CDS
TGAAGGTGAGACCGTAGTTC+CGG 0.422141 4_4:+62337661 None:intergenic
TGGGTCTTTCTCTTTATCCA+TGG 0.423723 4_4:+62336492 None:intergenic
TCCAATCCATCTCTTTGTTG+TGG 0.426159 4_4:-62335975 Msa0693430:three_prime_UTR
ATATTATGAAATGAATACTT+TGG 0.426551 4_4:-62336099 Msa0693430:three_prime_UTR
AGTTCTTCCCTTGAATATCA+TGG 0.429177 4_4:+62336750 None:intergenic
CGAGATTCGTAACTTAGAAA+AGG 0.436022 4_4:-62337823 Msa0693430:intron
TGAAGGCTTCCAAAGCAAAC+TGG 0.463661 4_4:-62336637 Msa0693430:CDS
GCATGGACTGATGCTAGAAC+TGG 0.466170 4_4:-62336234 Msa0693430:CDS
TTTGCACTTCAAAGAAGATA+AGG 0.467678 4_4:-62336609 Msa0693430:intron
AGACCCAATGGCAGGAATCA+TGG 0.469684 4_4:-62336476 Msa0693430:CDS
AGTCCATGATTCCTGCCATT+GGG 0.479183 4_4:+62336473 None:intergenic
ATTCCACCACCAATTTCAAC+CGG 0.485269 4_4:-62337680 Msa0693430:CDS
GTCAAGTTCCATGATATTCA+AGG 0.487655 4_4:-62336758 Msa0693430:CDS
CCCACAACAAAGAGATGGAT+TGG 0.488099 4_4:+62335974 None:intergenic
ACCAATTTCAACCGGAACTA+CGG 0.489576 4_4:-62337672 Msa0693430:CDS
TGCAGAATATGTACGAGGAT+GGG 0.496843 4_4:-62336287 Msa0693430:intron
TGCTCTGAGATTTGATCTCT+CGG 0.500672 4_4:+62335879 None:intergenic
GTAGTTCCGGTTGAAATTGG+TGG 0.503010 4_4:+62337674 None:intergenic
AAGTCCATGATTCCTGCCAT+TGG 0.506747 4_4:+62336472 None:intergenic
TAAAAGGGTAATCATCACTT+TGG 0.508151 4_4:-62336660 Msa0693430:CDS
TATGTAGATATATGTGTCAC+TGG 0.518048 4_4:-62336834 Msa0693430:intron
GCAGAATATGTACGAGGATG+GGG 0.520078 4_4:-62336286 Msa0693430:intron
CTCAACGATAGCTACTTAAA+GGG 0.526180 4_4:+62336199 None:intergenic
TAAGTACTCTGAGGATTGGT+GGG 0.532481 4_4:-62335928 Msa0693430:three_prime_UTR
TTGCAGAATATGTACGAGGA+TGG 0.536725 4_4:-62336288 Msa0693430:intron
GTTCCGGTTGAAATTGGTGG+TGG 0.541368 4_4:+62337677 None:intergenic
GAGATGGATTGGAGCGAACA+CGG 0.541899 4_4:+62335985 None:intergenic
AGGAATCATGGACTTGATGA+AGG 0.541947 4_4:-62336464 Msa0693430:intron
AGAGGAGAATAACTGAGTAC+AGG 0.548081 4_4:+62337638 None:intergenic
AGAAAACAATTGCAAAAGCA+TGG 0.549544 4_4:-62336251 Msa0693430:CDS
GTAAGTACTCTGAGGATTGG+TGG 0.555476 4_4:-62335929 Msa0693430:three_prime_UTR
GTGAGAAGAGAAAGGATTCG+AGG 0.555972 4_4:+62337849 None:intergenic
AAAGAGAAAGACCCAATGGC+AGG 0.557190 4_4:-62336484 Msa0693430:CDS
TCTCGGAAGTGAGAAGAGAA+AGG 0.558117 4_4:+62337841 None:intergenic
GTCGTTTCGCGATTTCGTGA+AGG 0.568105 4_4:+62337871 None:intergenic
GAGATCAAATCTCAGAGCAA+TGG 0.572431 4_4:-62335875 Msa0693430:three_prime_UTR
AAAGACCCACAACAAAGAGA+TGG 0.579888 4_4:+62335969 None:intergenic
GGTAATCATCACTTTGGTGA+AGG 0.580072 4_4:-62336654 Msa0693430:CDS
TTGAACATCTAAACCACTGT+AGG 0.584341 4_4:+62336173 None:intergenic
AGATGGATTGGAGCGAACAC+GGG 0.591261 4_4:+62335986 None:intergenic
AATTGAGCAGTTATCGTCTG+AGG 0.602215 4_4:-62337729 Msa0693430:intron
TATGTAAGTACTCTGAGGAT+TGG 0.605560 4_4:-62335932 Msa0693430:three_prime_UTR
TCAAGTTCCATGATATTCAA+GGG 0.618824 4_4:-62336757 Msa0693430:CDS
TGCAATAGTGAAATTGCACA+AGG 0.622130 4_4:-62336720 Msa0693430:CDS
GTTGCAGCTGAAGCCAGCCA+TGG 0.626054 4_4:-62336509 Msa0693430:intron
TCAGGATAACGACAAAGTCA+AGG 0.635580 4_4:-62337597 Msa0693430:intron
GAATAACTGAGTACAGGTGA+AGG 0.637142 4_4:+62337644 None:intergenic
GGATAAAGAGAAAGACCCAA+TGG 0.641682 4_4:-62336488 Msa0693430:CDS
TAGATATATGTGTCACTGGA+GGG 0.651331 4_4:-62336830 Msa0693430:CDS
TATCGTTGAGCAACCTACAG+TGG 0.651915 4_4:-62336186 Msa0693430:exon
TCCCAACTGAATGAAGCGAG+AGG 0.662613 4_4:+62337620 None:intergenic
TTGTATATGTAAGTACTCTG+AGG 0.663394 4_4:-62335937 Msa0693430:three_prime_UTR
GGAGGGTGTTGATGAGAGCA+AGG 0.664107 4_4:-62336813 Msa0693430:CDS
TGATGCTAGAACTGGAAAAG+CGG 0.666042 4_4:-62336226 Msa0693430:CDS
TGGAATCTGTGATGCACGTG+CGG 0.678359 4_4:+62337697 None:intergenic
GTAGATATATGTGTCACTGG+AGG 0.689960 4_4:-62336831 Msa0693430:intron
TGAATTGCAGAATATGTACG+AGG 0.745838 4_4:-62336292 Msa0693430:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAACAATACATCA+TGG + chr4_4:62336246-62336265 None:intergenic 15.0%
!! ATATTATGAAATGAATACTT+TGG - chr4_4:62337681-62337700 Msa0693430:CDS 15.0%
!! AAAATAATAACAATACATCA+TGG + chr4_4:62336246-62336265 None:intergenic 15.0%
!! ATATTATGAAATGAATACTT+TGG - chr4_4:62337681-62337700 Msa0693430:CDS 15.0%
!! AAATGATTAACGAAATGAAA+TGG + chr4_4:62336002-62336021 None:intergenic 20.0%
!! ATGATATAGGCATTTAATTA+AGG + chr4_4:62336350-62336369 None:intergenic 20.0%
!!! TCTATAAGCTATTTCTATTT+CGG - chr4_4:62336404-62336423 Msa0693430:intron 20.0%
!! AAATAAGCCAAAACAATTTA+TGG - chr4_4:62336504-62336523 Msa0693430:CDS 20.0%
!! TATAGCAAAGAATAAGATAA+AGG + chr4_4:62336854-62336873 None:intergenic 20.0%
!! TAAACTTAAACACTTATTCT+TGG - chr4_4:62337984-62338003 Msa0693430:five_prime_UTR 20.0%
!! AAATGATTAACGAAATGAAA+TGG + chr4_4:62336002-62336021 None:intergenic 20.0%
!! ATGATATAGGCATTTAATTA+AGG + chr4_4:62336350-62336369 None:intergenic 20.0%
!!! TCTATAAGCTATTTCTATTT+CGG - chr4_4:62336404-62336423 Msa0693430:intron 20.0%
!! AAATAAGCCAAAACAATTTA+TGG - chr4_4:62336504-62336523 Msa0693430:CDS 20.0%
!! TATAGCAAAGAATAAGATAA+AGG + chr4_4:62336854-62336873 None:intergenic 20.0%
!! TAAACTTAAACACTTATTCT+TGG - chr4_4:62337984-62338003 Msa0693430:five_prime_UTR 20.0%
!!! ATATGTGTCTGTTTTGATTT+TGG + chr4_4:62335793-62335812 None:intergenic 25.0%
! ATCGTATCATCGAAAAAAAA+TGG - chr4_4:62335849-62335868 Msa0693430:three_prime_UTR 25.0%
! ATAATTAGCACTAATCAAGA+GGG + chr4_4:62336026-62336045 None:intergenic 25.0%
! AATAATTAGCACTAATCAAG+AGG + chr4_4:62336027-62336046 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr4_4:62336640-62336659 None:intergenic 25.0%
! TACAAGATAGCTTACAAAAA+TGG + chr4_4:62336696-62336715 None:intergenic 25.0%
! TGAAACTGAAAAACACAAAT+TGG + chr4_4:62336827-62336846 None:intergenic 25.0%
! ATAAATCTTTGTATGCCATT+TGG + chr4_4:62336880-62336899 None:intergenic 25.0%
! ATTTATGTTGTAGTTACTCT+GGG - chr4_4:62336894-62336913 Msa0693430:intron 25.0%
!! ATTTACATTCTAAAGAGTAC+AGG + chr4_4:62337415-62337434 None:intergenic 25.0%
! ATATGCTTCACTTAATTTCA+TGG - chr4_4:62337649-62337668 Msa0693430:CDS 25.0%
!! TGGATTGAAAGATATGTTTT+TGG - chr4_4:62337925-62337944 Msa0693430:exon 25.0%
!! ATTCTGATGAACTTTTATCA+TGG - chr4_4:62337953-62337972 Msa0693430:five_prime_UTR 25.0%
!!! ATATGTGTCTGTTTTGATTT+TGG + chr4_4:62335793-62335812 None:intergenic 25.0%
! ATCGTATCATCGAAAAAAAA+TGG - chr4_4:62335849-62335868 Msa0693430:three_prime_UTR 25.0%
! ATAATTAGCACTAATCAAGA+GGG + chr4_4:62336026-62336045 None:intergenic 25.0%
! AATAATTAGCACTAATCAAG+AGG + chr4_4:62336027-62336046 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr4_4:62336640-62336659 None:intergenic 25.0%
! TACAAGATAGCTTACAAAAA+TGG + chr4_4:62336696-62336715 None:intergenic 25.0%
! TGAAACTGAAAAACACAAAT+TGG + chr4_4:62336827-62336846 None:intergenic 25.0%
! ATAAATCTTTGTATGCCATT+TGG + chr4_4:62336880-62336899 None:intergenic 25.0%
! ATTTATGTTGTAGTTACTCT+GGG - chr4_4:62336894-62336913 Msa0693430:intron 25.0%
!! ATTTACATTCTAAAGAGTAC+AGG + chr4_4:62337415-62337434 None:intergenic 25.0%
! ATATGCTTCACTTAATTTCA+TGG - chr4_4:62337649-62337668 Msa0693430:CDS 25.0%
!! TGGATTGAAAGATATGTTTT+TGG - chr4_4:62337925-62337944 Msa0693430:exon 25.0%
!! ATTCTGATGAACTTTTATCA+TGG - chr4_4:62337953-62337972 Msa0693430:five_prime_UTR 25.0%
ACATATATACACCGACAAAA+AGG - chr4_4:62335807-62335826 Msa0693430:three_prime_UTR 30.0%
! AATGAAACTCTCCTTTTTGT+CGG + chr4_4:62335821-62335840 None:intergenic 30.0%
! CTTTTCTAAGTTACGAATCT+CGG + chr4_4:62335959-62335978 None:intergenic 30.0%
TAATTAGCACTAATCAAGAG+GGG + chr4_4:62336025-62336044 None:intergenic 30.0%
! TCTTTCATTTCATACCCTTT+TGG - chr4_4:62336273-62336292 Msa0693430:CDS 30.0%
! GTTAAGTTGTTTATCCAAAC+AGG + chr4_4:62336641-62336660 None:intergenic 30.0%
! ATAAGCTGAATGTTTTGCAA+AGG - chr4_4:62336757-62336776 Msa0693430:CDS 30.0%
! TTCTTTGCTATAGATCCAAA+TGG - chr4_4:62336862-62336881 Msa0693430:intron 30.0%
GATTTATGTTGTAGTTACTC+TGG - chr4_4:62336893-62336912 Msa0693430:intron 30.0%
TATGTAGATATATGTGTCAC+TGG - chr4_4:62336946-62336965 Msa0693430:intron 30.0%
AACTTGACATCAAACGAATT+TGG + chr4_4:62337010-62337029 None:intergenic 30.0%
TCAAGTTCCATGATATTCAA+GGG - chr4_4:62337023-62337042 Msa0693430:intron 30.0%
! ACCAGAGAAATGTAAGATTT+TGG - chr4_4:62337090-62337109 Msa0693430:intron 30.0%
ACCAAAATCTTACATTTCTC+TGG + chr4_4:62337094-62337113 None:intergenic 30.0%
! AAAGTGATGATTACCCTTTT+AGG + chr4_4:62337121-62337140 None:intergenic 30.0%
TAAAAGGGTAATCATCACTT+TGG - chr4_4:62337120-62337139 Msa0693430:intron 30.0%
TTTGCACTTCAAAGAAGATA+AGG - chr4_4:62337171-62337190 Msa0693430:intron 30.0%
AGAAAACAATTGCAAAAGCA+TGG - chr4_4:62337529-62337548 Msa0693430:intron 30.0%
! AGATGTTCAATTCCTGTTTT+TGG - chr4_4:62337620-62337639 Msa0693430:CDS 30.0%
! TTTTAGATACATTGCACTGT+AGG - chr4_4:62337711-62337730 Msa0693430:CDS 30.0%
TTGTATATGTAAGTACTCTG+AGG - chr4_4:62337843-62337862 Msa0693430:CDS 30.0%
ACATATATACACCGACAAAA+AGG - chr4_4:62335807-62335826 Msa0693430:three_prime_UTR 30.0%
! AATGAAACTCTCCTTTTTGT+CGG + chr4_4:62335821-62335840 None:intergenic 30.0%
! CTTTTCTAAGTTACGAATCT+CGG + chr4_4:62335959-62335978 None:intergenic 30.0%
TAATTAGCACTAATCAAGAG+GGG + chr4_4:62336025-62336044 None:intergenic 30.0%
! TCTTTCATTTCATACCCTTT+TGG - chr4_4:62336273-62336292 Msa0693430:CDS 30.0%
! GTTAAGTTGTTTATCCAAAC+AGG + chr4_4:62336641-62336660 None:intergenic 30.0%
! ATAAGCTGAATGTTTTGCAA+AGG - chr4_4:62336757-62336776 Msa0693430:CDS 30.0%
! TTCTTTGCTATAGATCCAAA+TGG - chr4_4:62336862-62336881 Msa0693430:intron 30.0%
GATTTATGTTGTAGTTACTC+TGG - chr4_4:62336893-62336912 Msa0693430:intron 30.0%
TATGTAGATATATGTGTCAC+TGG - chr4_4:62336946-62336965 Msa0693430:intron 30.0%
AACTTGACATCAAACGAATT+TGG + chr4_4:62337010-62337029 None:intergenic 30.0%
TCAAGTTCCATGATATTCAA+GGG - chr4_4:62337023-62337042 Msa0693430:intron 30.0%
! ACCAGAGAAATGTAAGATTT+TGG - chr4_4:62337090-62337109 Msa0693430:intron 30.0%
ACCAAAATCTTACATTTCTC+TGG + chr4_4:62337094-62337113 None:intergenic 30.0%
! AAAGTGATGATTACCCTTTT+AGG + chr4_4:62337121-62337140 None:intergenic 30.0%
TAAAAGGGTAATCATCACTT+TGG - chr4_4:62337120-62337139 Msa0693430:intron 30.0%
TTTGCACTTCAAAGAAGATA+AGG - chr4_4:62337171-62337190 Msa0693430:intron 30.0%
AGAAAACAATTGCAAAAGCA+TGG - chr4_4:62337529-62337548 Msa0693430:intron 30.0%
! AGATGTTCAATTCCTGTTTT+TGG - chr4_4:62337620-62337639 Msa0693430:CDS 30.0%
! TTTTAGATACATTGCACTGT+AGG - chr4_4:62337711-62337730 Msa0693430:CDS 30.0%
TTGTATATGTAAGTACTCTG+AGG - chr4_4:62337843-62337862 Msa0693430:CDS 30.0%
!!! GTGTCTGTTTTGATTTTGGA+AGG + chr4_4:62335789-62335808 None:intergenic 35.0%
TCATCGAAAAAAAATGGCAG+AGG - chr4_4:62335855-62335874 Msa0693430:three_prime_UTR 35.0%
! AGAGGAATTCGCTTTAGATT+TGG - chr4_4:62335873-62335892 Msa0693430:three_prime_UTR 35.0%
CGAGATTCGTAACTTAGAAA+AGG - chr4_4:62335957-62335976 Msa0693430:three_prime_UTR 35.0%
! TCATACCCTTTTGGATTGAA+AGG - chr4_4:62336282-62336301 Msa0693430:CDS 35.0%
! CATACCCTTTTGGATTGAAA+GGG - chr4_4:62336283-62336302 Msa0693430:CDS 35.0%
TCGATCAGTGTCTATGATAT+AGG + chr4_4:62336363-62336382 None:intergenic 35.0%
!! CTTATTTTCACAAGCTCTTC+GGG + chr4_4:62336491-62336510 None:intergenic 35.0%
!! GCTTATTTTCACAAGCTCTT+CGG + chr4_4:62336492-62336511 None:intergenic 35.0%
! CTCGTGTCCATAAATTGTTT+TGG + chr4_4:62336514-62336533 None:intergenic 35.0%
CATGTCGTAAATGCTAAGAA+TGG - chr4_4:62336614-62336633 Msa0693430:CDS 35.0%
AAATGAGCCAATACAAACAG+AGG - chr4_4:62336801-62336820 Msa0693430:CDS 35.0%
! AAATTGGCCTCTGTTTGTAT+TGG + chr4_4:62336811-62336830 None:intergenic 35.0%
! TAGATATATGTGTCACTGGA+GGG - chr4_4:62336950-62336969 Msa0693430:intron 35.0%
GTCAAGTTCCATGATATTCA+AGG - chr4_4:62337022-62337041 Msa0693430:intron 35.0%
AGTTCTTCCCTTGAATATCA+TGG + chr4_4:62337033-62337052 None:intergenic 35.0%
TGCAATAGTGAAATTGCACA+AGG - chr4_4:62337060-62337079 Msa0693430:intron 35.0%
!! AGATTTTGGTCAAGCCTAAA+AGG - chr4_4:62337104-62337123 Msa0693430:intron 35.0%
!! GATTTTGGTCAAGCCTAAAA+GGG - chr4_4:62337105-62337124 Msa0693430:intron 35.0%
TGAATTGCAGAATATGTACG+AGG - chr4_4:62337488-62337507 Msa0693430:intron 35.0%
CTCAACGATAGCTACTTAAA+GGG + chr4_4:62337584-62337603 None:intergenic 35.0%
TTGAACATCTAAACCACTGT+AGG + chr4_4:62337610-62337629 None:intergenic 35.0%
! TATGTAAGTACTCTGAGGAT+TGG - chr4_4:62337848-62337867 Msa0693430:CDS 35.0%
!!! GTGTCTGTTTTGATTTTGGA+AGG + chr4_4:62335789-62335808 None:intergenic 35.0%
TCATCGAAAAAAAATGGCAG+AGG - chr4_4:62335855-62335874 Msa0693430:three_prime_UTR 35.0%
! AGAGGAATTCGCTTTAGATT+TGG - chr4_4:62335873-62335892 Msa0693430:three_prime_UTR 35.0%
CGAGATTCGTAACTTAGAAA+AGG - chr4_4:62335957-62335976 Msa0693430:three_prime_UTR 35.0%
! TCATACCCTTTTGGATTGAA+AGG - chr4_4:62336282-62336301 Msa0693430:CDS 35.0%
! CATACCCTTTTGGATTGAAA+GGG - chr4_4:62336283-62336302 Msa0693430:CDS 35.0%
TCGATCAGTGTCTATGATAT+AGG + chr4_4:62336363-62336382 None:intergenic 35.0%
!! CTTATTTTCACAAGCTCTTC+GGG + chr4_4:62336491-62336510 None:intergenic 35.0%
!! GCTTATTTTCACAAGCTCTT+CGG + chr4_4:62336492-62336511 None:intergenic 35.0%
! CTCGTGTCCATAAATTGTTT+TGG + chr4_4:62336514-62336533 None:intergenic 35.0%
CATGTCGTAAATGCTAAGAA+TGG - chr4_4:62336614-62336633 Msa0693430:CDS 35.0%
AAATGAGCCAATACAAACAG+AGG - chr4_4:62336801-62336820 Msa0693430:CDS 35.0%
! AAATTGGCCTCTGTTTGTAT+TGG + chr4_4:62336811-62336830 None:intergenic 35.0%
! TAGATATATGTGTCACTGGA+GGG - chr4_4:62336950-62336969 Msa0693430:intron 35.0%
GTCAAGTTCCATGATATTCA+AGG - chr4_4:62337022-62337041 Msa0693430:intron 35.0%
AGTTCTTCCCTTGAATATCA+TGG + chr4_4:62337033-62337052 None:intergenic 35.0%
TGCAATAGTGAAATTGCACA+AGG - chr4_4:62337060-62337079 Msa0693430:intron 35.0%
!! AGATTTTGGTCAAGCCTAAA+AGG - chr4_4:62337104-62337123 Msa0693430:intron 35.0%
!! GATTTTGGTCAAGCCTAAAA+GGG - chr4_4:62337105-62337124 Msa0693430:intron 35.0%
TGAATTGCAGAATATGTACG+AGG - chr4_4:62337488-62337507 Msa0693430:intron 35.0%
CTCAACGATAGCTACTTAAA+GGG + chr4_4:62337584-62337603 None:intergenic 35.0%
TTGAACATCTAAACCACTGT+AGG + chr4_4:62337610-62337629 None:intergenic 35.0%
! TATGTAAGTACTCTGAGGAT+TGG - chr4_4:62337848-62337867 Msa0693430:CDS 35.0%
AATTGAGCAGTTATCGTCTG+AGG - chr4_4:62336051-62336070 Msa0693430:three_prime_UTR 40.0%
ATTCCACCACCAATTTCAAC+CGG - chr4_4:62336100-62336119 Msa0693430:three_prime_UTR 40.0%
ACCAATTTCAACCGGAACTA+CGG - chr4_4:62336108-62336127 Msa0693430:three_prime_UTR 40.0%
GAATAACTGAGTACAGGTGA+AGG + chr4_4:62336139-62336158 None:intergenic 40.0%
AGAGGAGAATAACTGAGTAC+AGG + chr4_4:62336145-62336164 None:intergenic 40.0%
TCAGGATAACGACAAAGTCA+AGG - chr4_4:62336183-62336202 Msa0693430:exon 40.0%
CGTACCCTTTCAATCCAAAA+GGG + chr4_4:62336290-62336309 None:intergenic 40.0%
TCGTACCCTTTCAATCCAAA+AGG + chr4_4:62336291-62336310 None:intergenic 40.0%
AGACTGTTCAGAAGAACTAG+TGG + chr4_4:62336548-62336567 None:intergenic 40.0%
GCTCATTTGAGCTTATCTAC+TGG + chr4_4:62336789-62336808 None:intergenic 40.0%
! GTAGATATATGTGTCACTGG+AGG - chr4_4:62336949-62336968 Msa0693430:intron 40.0%
GGTAATCATCACTTTGGTGA+AGG - chr4_4:62337126-62337145 Msa0693430:intron 40.0%
! CAAATCTAGCCAGTTTGCTT+TGG + chr4_4:62337155-62337174 None:intergenic 40.0%
TCTTTCTCTTTATCCATGGC+TGG + chr4_4:62337287-62337306 None:intergenic 40.0%
TGGGTCTTTCTCTTTATCCA+TGG + chr4_4:62337291-62337310 None:intergenic 40.0%
GGATAAAGAGAAAGACCCAA+TGG - chr4_4:62337292-62337311 Msa0693430:intron 40.0%
AGGAATCATGGACTTGATGA+AGG - chr4_4:62337316-62337335 Msa0693430:intron 40.0%
TTGCAGAATATGTACGAGGA+TGG - chr4_4:62337492-62337511 Msa0693430:intron 40.0%
TGCAGAATATGTACGAGGAT+GGG - chr4_4:62337493-62337512 Msa0693430:intron 40.0%
TGATGCTAGAACTGGAAAAG+CGG - chr4_4:62337554-62337573 Msa0693430:intron 40.0%
GCTCAACGATAGCTACTTAA+AGG + chr4_4:62337585-62337604 None:intergenic 40.0%
GCATATCACTCTCCAAAAAC+AGG + chr4_4:62337635-62337654 None:intergenic 40.0%
TCCAATCCATCTCTTTGTTG+TGG - chr4_4:62337805-62337824 Msa0693430:intron 40.0%
! CCAATCCATCTCTTTGTTGT+GGG - chr4_4:62337806-62337825 Msa0693430:intron 40.0%
AAAGACCCACAACAAAGAGA+TGG + chr4_4:62337814-62337833 None:intergenic 40.0%
! TAAGTACTCTGAGGATTGGT+GGG - chr4_4:62337852-62337871 Msa0693430:CDS 40.0%
!! TGCTCTGAGATTTGATCTCT+CGG + chr4_4:62337904-62337923 None:intergenic 40.0%
GAGATCAAATCTCAGAGCAA+TGG - chr4_4:62337905-62337924 Msa0693430:CDS 40.0%
!! TATTCTTGGTGCTGTAGTCT+AGG - chr4_4:62337998-62338017 Msa0693430:five_prime_UTR 40.0%
AATTGAGCAGTTATCGTCTG+AGG - chr4_4:62336051-62336070 Msa0693430:three_prime_UTR 40.0%
ATTCCACCACCAATTTCAAC+CGG - chr4_4:62336100-62336119 Msa0693430:three_prime_UTR 40.0%
ACCAATTTCAACCGGAACTA+CGG - chr4_4:62336108-62336127 Msa0693430:three_prime_UTR 40.0%
GAATAACTGAGTACAGGTGA+AGG + chr4_4:62336139-62336158 None:intergenic 40.0%
AGAGGAGAATAACTGAGTAC+AGG + chr4_4:62336145-62336164 None:intergenic 40.0%
TCAGGATAACGACAAAGTCA+AGG - chr4_4:62336183-62336202 Msa0693430:exon 40.0%
CGTACCCTTTCAATCCAAAA+GGG + chr4_4:62336290-62336309 None:intergenic 40.0%
TCGTACCCTTTCAATCCAAA+AGG + chr4_4:62336291-62336310 None:intergenic 40.0%
AGACTGTTCAGAAGAACTAG+TGG + chr4_4:62336548-62336567 None:intergenic 40.0%
GCTCATTTGAGCTTATCTAC+TGG + chr4_4:62336789-62336808 None:intergenic 40.0%
! GTAGATATATGTGTCACTGG+AGG - chr4_4:62336949-62336968 Msa0693430:intron 40.0%
GGTAATCATCACTTTGGTGA+AGG - chr4_4:62337126-62337145 Msa0693430:intron 40.0%
! CAAATCTAGCCAGTTTGCTT+TGG + chr4_4:62337155-62337174 None:intergenic 40.0%
TCTTTCTCTTTATCCATGGC+TGG + chr4_4:62337287-62337306 None:intergenic 40.0%
TGGGTCTTTCTCTTTATCCA+TGG + chr4_4:62337291-62337310 None:intergenic 40.0%
GGATAAAGAGAAAGACCCAA+TGG - chr4_4:62337292-62337311 Msa0693430:intron 40.0%
AGGAATCATGGACTTGATGA+AGG - chr4_4:62337316-62337335 Msa0693430:intron 40.0%
TTGCAGAATATGTACGAGGA+TGG - chr4_4:62337492-62337511 Msa0693430:intron 40.0%
TGCAGAATATGTACGAGGAT+GGG - chr4_4:62337493-62337512 Msa0693430:intron 40.0%
TGATGCTAGAACTGGAAAAG+CGG - chr4_4:62337554-62337573 Msa0693430:intron 40.0%
GCTCAACGATAGCTACTTAA+AGG + chr4_4:62337585-62337604 None:intergenic 40.0%
GCATATCACTCTCCAAAAAC+AGG + chr4_4:62337635-62337654 None:intergenic 40.0%
TCCAATCCATCTCTTTGTTG+TGG - chr4_4:62337805-62337824 Msa0693430:intron 40.0%
! CCAATCCATCTCTTTGTTGT+GGG - chr4_4:62337806-62337825 Msa0693430:intron 40.0%
AAAGACCCACAACAAAGAGA+TGG + chr4_4:62337814-62337833 None:intergenic 40.0%
! TAAGTACTCTGAGGATTGGT+GGG - chr4_4:62337852-62337871 Msa0693430:CDS 40.0%
!! TGCTCTGAGATTTGATCTCT+CGG + chr4_4:62337904-62337923 None:intergenic 40.0%
GAGATCAAATCTCAGAGCAA+TGG - chr4_4:62337905-62337924 Msa0693430:CDS 40.0%
GTGAGAAGAGAAAGGATTCG+AGG + chr4_4:62335934-62335953 None:intergenic 45.0%
TCTCGGAAGTGAGAAGAGAA+AGG + chr4_4:62335942-62335961 None:intergenic 45.0%
GTAGTTCCGGTTGAAATTGG+TGG + chr4_4:62336109-62336128 None:intergenic 45.0%
ACCGTAGTTCCGGTTGAAAT+TGG + chr4_4:62336112-62336131 None:intergenic 45.0%
TCCTCTCGCTTCATTCAGTT+GGG - chr4_4:62336159-62336178 Msa0693430:three_prime_UTR 45.0%
ATGCTAAGAATGGCCCTGTT+TGG - chr4_4:62336624-62336643 Msa0693430:CDS 45.0%
TGAAGGCTTCCAAAGCAAAC+TGG - chr4_4:62337143-62337162 Msa0693430:intron 45.0%
AAAGAGAAAGACCCAATGGC+AGG - chr4_4:62337296-62337315 Msa0693430:intron 45.0%
AGTCCATGATTCCTGCCATT+GGG + chr4_4:62337310-62337329 None:intergenic 45.0%
AAGTCCATGATTCCTGCCAT+TGG + chr4_4:62337311-62337330 None:intergenic 45.0%
GCAGAATATGTACGAGGATG+GGG - chr4_4:62337494-62337513 Msa0693430:intron 45.0%
TATCGTTGAGCAACCTACAG+TGG - chr4_4:62337594-62337613 Msa0693430:intron 45.0%
CCCACAACAAAGAGATGGAT+TGG + chr4_4:62337809-62337828 None:intergenic 45.0%
! GTAAGTACTCTGAGGATTGG+TGG - chr4_4:62337851-62337870 Msa0693430:CDS 45.0%
GTGAGAAGAGAAAGGATTCG+AGG + chr4_4:62335934-62335953 None:intergenic 45.0%
TCTCGGAAGTGAGAAGAGAA+AGG + chr4_4:62335942-62335961 None:intergenic 45.0%
GTAGTTCCGGTTGAAATTGG+TGG + chr4_4:62336109-62336128 None:intergenic 45.0%
ACCGTAGTTCCGGTTGAAAT+TGG + chr4_4:62336112-62336131 None:intergenic 45.0%
TCCTCTCGCTTCATTCAGTT+GGG - chr4_4:62336159-62336178 Msa0693430:three_prime_UTR 45.0%
ATGCTAAGAATGGCCCTGTT+TGG - chr4_4:62336624-62336643 Msa0693430:CDS 45.0%
TGAAGGCTTCCAAAGCAAAC+TGG - chr4_4:62337143-62337162 Msa0693430:intron 45.0%
AAAGAGAAAGACCCAATGGC+AGG - chr4_4:62337296-62337315 Msa0693430:intron 45.0%
AGTCCATGATTCCTGCCATT+GGG + chr4_4:62337310-62337329 None:intergenic 45.0%
AAGTCCATGATTCCTGCCAT+TGG + chr4_4:62337311-62337330 None:intergenic 45.0%
GCAGAATATGTACGAGGATG+GGG - chr4_4:62337494-62337513 Msa0693430:intron 45.0%
TATCGTTGAGCAACCTACAG+TGG - chr4_4:62337594-62337613 Msa0693430:intron 45.0%
CCCACAACAAAGAGATGGAT+TGG + chr4_4:62337809-62337828 None:intergenic 45.0%
! GTAAGTACTCTGAGGATTGG+TGG - chr4_4:62337851-62337870 Msa0693430:CDS 45.0%
GTCGTTTCGCGATTTCGTGA+AGG + chr4_4:62335912-62335931 None:intergenic 50.0%
TGGAATCTGTGATGCACGTG+CGG + chr4_4:62336086-62336105 None:intergenic 50.0%
GTTCCGGTTGAAATTGGTGG+TGG + chr4_4:62336106-62336125 None:intergenic 50.0%
TGAAGGTGAGACCGTAGTTC+CGG + chr4_4:62336122-62336141 None:intergenic 50.0%
CTCCTCTCGCTTCATTCAGT+TGG - chr4_4:62336158-62336177 Msa0693430:three_prime_UTR 50.0%
TCCCAACTGAATGAAGCGAG+AGG + chr4_4:62336163-62336182 None:intergenic 50.0%
CGCTTCATTCAGTTGGGATC+AGG - chr4_4:62336165-62336184 Msa0693430:three_prime_UTR 50.0%
AGACCCAATGGCAGGAATCA+TGG - chr4_4:62337304-62337323 Msa0693430:intron 50.0%
! GCATGGACTGATGCTAGAAC+TGG - chr4_4:62337546-62337565 Msa0693430:intron 50.0%
!! AGATGGATTGGAGCGAACAC+GGG + chr4_4:62337797-62337816 None:intergenic 50.0%
!! GAGATGGATTGGAGCGAACA+CGG + chr4_4:62337798-62337817 None:intergenic 50.0%
GTCGTTTCGCGATTTCGTGA+AGG + chr4_4:62335912-62335931 None:intergenic 50.0%
TGGAATCTGTGATGCACGTG+CGG + chr4_4:62336086-62336105 None:intergenic 50.0%
GTTCCGGTTGAAATTGGTGG+TGG + chr4_4:62336106-62336125 None:intergenic 50.0%
TGAAGGTGAGACCGTAGTTC+CGG + chr4_4:62336122-62336141 None:intergenic 50.0%
CTCCTCTCGCTTCATTCAGT+TGG - chr4_4:62336158-62336177 Msa0693430:three_prime_UTR 50.0%
TCCCAACTGAATGAAGCGAG+AGG + chr4_4:62336163-62336182 None:intergenic 50.0%
CGCTTCATTCAGTTGGGATC+AGG - chr4_4:62336165-62336184 Msa0693430:three_prime_UTR 50.0%
AGACCCAATGGCAGGAATCA+TGG - chr4_4:62337304-62337323 Msa0693430:intron 50.0%
! GCATGGACTGATGCTAGAAC+TGG - chr4_4:62337546-62337565 Msa0693430:intron 50.0%
!! AGATGGATTGGAGCGAACAC+GGG + chr4_4:62337797-62337816 None:intergenic 50.0%
!! GAGATGGATTGGAGCGAACA+CGG + chr4_4:62337798-62337817 None:intergenic 50.0%
!! GGAGGGTGTTGATGAGAGCA+AGG - chr4_4:62336967-62336986 Msa0693430:intron 55.0%
!! GGAGGGTGTTGATGAGAGCA+AGG - chr4_4:62336967-62336986 Msa0693430:intron 55.0%
GTTGCAGCTGAAGCCAGCCA+TGG - chr4_4:62337271-62337290 Msa0693430:intron 60.0%
GTTGCAGCTGAAGCCAGCCA+TGG - chr4_4:62337271-62337290 Msa0693430:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 62335783 62338019 62335783 ID=Msa0693430;Name=Msa0693430
chr4_4 mRNA 62335783 62338019 62335783 ID=Msa0693430-mRNA-1;Parent=Msa0693430;Name=Msa0693430-mRNA-1;_AED=0.04;_eAED=0.04;_QI=85|1|1|1|1|1|5|417|219
chr4_4 exon 62335783 62336304 62335783 ID=Msa0693430-mRNA-1:exon:17444;Parent=Msa0693430-mRNA-1
chr4_4 exon 62336465 62336524 62336465 ID=Msa0693430-mRNA-1:exon:17443;Parent=Msa0693430-mRNA-1
chr4_4 exon 62336610 62336849 62336610 ID=Msa0693430-mRNA-1:exon:17442;Parent=Msa0693430-mRNA-1
chr4_4 exon 62337598 62337741 62337598 ID=Msa0693430-mRNA-1:exon:17441;Parent=Msa0693430-mRNA-1
chr4_4 exon 62337824 62338019 62337824 ID=Msa0693430-mRNA-1:exon:17440;Parent=Msa0693430-mRNA-1
chr4_4 five_prime_UTR 62337935 62338019 62337935 ID=Msa0693430-mRNA-1:five_prime_utr;Parent=Msa0693430-mRNA-1
chr4_4 CDS 62337824 62337934 62337824 ID=Msa0693430-mRNA-1:cds;Parent=Msa0693430-mRNA-1
chr4_4 CDS 62337598 62337741 62337598 ID=Msa0693430-mRNA-1:cds;Parent=Msa0693430-mRNA-1
chr4_4 CDS 62336610 62336849 62336610 ID=Msa0693430-mRNA-1:cds;Parent=Msa0693430-mRNA-1
chr4_4 CDS 62336465 62336524 62336465 ID=Msa0693430-mRNA-1:cds;Parent=Msa0693430-mRNA-1
chr4_4 CDS 62336200 62336304 62336200 ID=Msa0693430-mRNA-1:cds;Parent=Msa0693430-mRNA-1
chr4_4 three_prime_UTR 62335783 62336199 62335783 ID=Msa0693430-mRNA-1:three_prime_utr;Parent=Msa0693430-mRNA-1
Gene Sequence

>Msa0693430

ATGGCAGAGGAATTCGCTTTAGATTTGGAAGAGCTTCGTCACCTTCACGAAATCGCGAAACGACCTCGAATCCTTTCTCTTCTCACTTCCGAGATTCGTAACTTAGAAAAGTTATCGTCTGAGGCAACATCTACCGCACGTGCATCACAGATTCCACCACCAATTTCAACCGGAACTACGGTCTCACCTTCACCTGTACTCAGTTATTCTCCTCTCGCTTCATTCAGTTGGGATCAGGATAACGACAAAGTCAAGATATATGTGTCACTGGAGGGTGTTGATGAGAGCAAGGTTGAATCTGAGTTTAAGCCAAATTCGTTTGATGTCAAGTTCCATGATATTCAAGGGAAGAACTATCGATTTGCAATAGTGAAATTGCACAAGGAGATAGTACCAGAGAAATGTAAGATTTTGGTCAAGCCTAAAAGGGTAATCATCACTTTGGTGAAGGCTTCCAAAGCAAACTGGCTAGATTTGCACTTCAAAGAAGATAAGCTGAAGCCAGCCATGGATAAAGAGAAAGACCCAATGGCAGGAATCATGGACTTGATGAAGAATATGTACGAGGATGGGGACGAAGAGATGAAGAAAACAATTGCAAAAGCATGGACTGATGCTAGAACTGGAAAAGCGGCTGACCCTTTAAGTAGCTATCGTTGA
Protein sequence

>Msa0693430

MAEEFALDLEELRHLHEIAKRPRILSLLTSEIRNLEKLSSEATSTARASQIPPPISTGTTVSPSPVLSYSPLASFSWDQDNDKVKIYVSLEGVDESKVESEFKPNSFDVKFHDIQGKNYRFAIVKLHKEIVPEKCKILVKPKRVIITLVKASKANWLDLHFKEDKLKPAMDKEKDPMAGIMDLMKNMYEDGDEEMKKTIAKAWTDARTGKAADPLSSYR*