AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708770 MtrunA17_Chr2g0322181 99.520 417 2 0 1 417 1 417 0.0 858
Msa0708770 MtrunA17_Chr4g0025401 91.169 419 32 3 1 417 1 416 0.0 686
Msa0708770 MtrunA17_Chr8g0334811 70.991 424 115 5 1 417 1 423 0.0 582
Msa0708770 MtrunA17_Chr7g0269841 37.322 351 180 9 14 358 27 343 2.18e-58 194
Msa0708770 MtrunA17_Chr4g0040501 33.333 360 204 10 14 358 5 343 7.01e-51 174
Msa0708770 MtrunA17_Chr2g0333221 32.143 364 221 11 11 357 68 422 2.86e-49 174
Msa0708770 MtrunA17_Chr4g0010781 34.463 354 186 11 14 347 87 414 1.25e-48 171
Msa0708770 MtrunA17_Chr3g0123861 33.144 353 217 9 14 357 83 425 2.45e-48 170
Msa0708770 MtrunA17_Chr2g0324921 32.234 394 154 9 14 358 5 334 2.35e-47 165
Msa0708770 MtrunA17_Chr2g0282741 33.333 360 199 11 14 358 5 338 3.74e-47 164
Msa0708770 MtrunA17_Chr3g0138471 32.213 357 194 7 14 358 5 325 4.37e-46 161
Msa0708770 MtrunA17_Chr6g0478651 31.793 368 225 11 14 365 69 426 5.87e-46 166
Msa0708770 MtrunA17_Chr2g0282761 34.018 341 199 8 14 343 5 330 1.61e-45 160
Msa0708770 MtrunA17_Chr4g0006391 30.151 398 161 9 14 358 5 338 7.86e-40 145
Msa0708770 MtrunA17_Chr3g0087741 30.645 372 212 15 1 355 80 422 4.40e-39 145
Msa0708770 MtrunA17_Chr5g0426951 27.957 372 221 11 5 355 75 420 4.14e-31 124
Msa0708770 MtrunA17_Chr5g0415751 36.552 145 90 1 214 358 18 160 2.65e-28 109
Msa0708770 MtrunA17_Chr3g0093111 34.752 141 90 1 218 358 172 310 1.34e-21 94.7
Msa0708770 MtrunA17_Chr3g0093111 27.136 199 108 5 14 180 3 196 6.77e-16 78.2
Msa0708770 MtrunA17_Chr3g0124801 34.483 145 92 2 215 358 149 291 4.08e-19 87.0
Msa0708770 MtrunA17_Chr2g0277721 32.639 144 96 1 215 358 117 259 4.80e-18 83.6
Msa0708770 MtrunA17_Chr8g0352451 46.392 97 43 3 12 104 71 162 4.31e-15 76.6
Msa0708770 MtrunA17_Chr7g0263971 52.564 78 31 2 4 76 360 436 3.01e-14 74.3
Msa0708770 MtrunA17_Chr5g0399201 43.750 80 38 1 10 82 9 88 3.68e-14 74.3
Msa0708770 MtrunA17_Chr1g0209551 52.941 68 28 1 15 78 18 85 8.18e-14 72.8
Msa0708770 MtrunA17_Chr1g0159511 43.878 98 44 4 12 104 5 96 2.14e-13 71.2
Msa0708770 MtrunA17_Chr1g0159491 48.571 70 32 2 13 78 6 75 5.92e-13 63.9
Msa0708770 MtrunA17_Chr5g0393371 40.206 97 49 3 12 104 5 96 1.94e-12 68.6
Msa0708770 MtrunA17_Chr5g0415731 32.215 149 63 5 11 146 3 126 3.20e-12 63.9
Msa0708770 MtrunA17_Chr5g0414471 37.255 102 50 3 15 105 27 125 1.14e-11 66.2
Msa0708770 MtrunA17_Chr7g0271891 40.000 75 37 1 14 80 17 91 3.61e-11 61.6
Msa0708770 MtrunA17_Chr3g0091361 47.297 74 34 2 14 82 6 79 4.37e-11 62.4
Msa0708770 MtrunA17_Chr7g0242951 46.154 65 32 1 15 76 29 93 7.81e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708770 AT5G22060.1 83.055 419 69 2 1 417 1 419 0.0 684
Msa0708770 AT3G44110.1 85.036 421 58 3 1 417 1 420 0.0 655
Msa0708770 AT3G44110.2 87.651 332 39 2 1 331 1 331 0.0 530
Msa0708770 AT3G62600.1 35.775 355 182 11 14 358 27 345 7.75e-56 188
Msa0708770 AT1G80030.2 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0708770 AT1G80030.3 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0708770 AT1G80030.1 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0708770 AT1G80030.4 32.964 361 216 10 14 357 76 427 6.91e-53 184
Msa0708770 AT2G20560.1 33.711 353 205 8 14 358 5 336 8.21e-50 172
Msa0708770 AT5G25530.2 32.597 362 198 9 14 358 5 337 9.30e-48 166
Msa0708770 AT5G48030.1 34.167 360 201 13 14 355 95 436 1.04e-47 169
Msa0708770 AT2G22360.1 32.075 371 229 10 14 378 87 440 5.79e-47 167
Msa0708770 AT4G39960.1 31.198 359 228 9 11 357 83 434 6.08e-45 162
Msa0708770 AT4G39960.2 31.198 359 228 9 11 357 83 434 6.08e-45 162
Msa0708770 AT5G25530.1 31.892 370 198 10 14 358 5 345 6.85e-45 159
Msa0708770 AT4G28480.1 31.198 359 217 8 14 358 5 347 1.28e-44 158
Msa0708770 AT3G08910.1 30.789 380 164 9 14 358 5 320 9.56e-40 145
Msa0708770 AT3G17830.1 30.303 363 225 10 12 355 62 415 5.42e-38 144
Msa0708770 AT5G01390.1 29.517 393 165 11 14 358 5 333 9.32e-38 140
Msa0708770 AT1G28210.3 27.895 380 229 11 7 368 42 394 1.08e-33 130
Msa0708770 AT1G28210.1 27.895 380 229 11 7 368 42 394 1.50e-33 130
Msa0708770 AT1G28210.2 27.895 380 229 11 7 368 42 394 1.84e-33 130
Msa0708770 AT2G20550.1 37.241 145 89 1 214 358 141 283 1.06e-26 108
Msa0708770 AT2G20550.2 37.241 145 89 1 214 358 141 283 1.06e-26 108
Msa0708770 AT3G47940.1 36.552 145 90 1 214 358 204 346 1.82e-26 109
Msa0708770 AT3G47940.1 34.021 194 67 4 14 146 5 198 1.12e-20 93.2
Msa0708770 AT5G01390.2 35.507 138 87 1 221 358 140 275 1.11e-23 100
Msa0708770 AT5G01390.2 40.741 108 43 3 14 100 5 112 1.78e-16 79.7
Msa0708770 AT4G28480.2 34.694 147 92 2 212 358 147 289 1.68e-23 100
Msa0708770 AT4G28480.2 43.750 112 50 2 14 120 5 108 6.04e-18 84.3
Msa0708770 AT1G59725.1 36.957 138 85 1 221 358 193 328 7.45e-23 99.0
Msa0708770 AT1G59725.1 32.955 176 75 4 14 146 5 180 4.32e-19 88.2
Msa0708770 AT1G10350.1 36.552 145 87 3 214 358 207 346 7.94e-23 99.4
Msa0708770 AT1G10350.1 32.308 195 70 6 14 146 5 199 3.69e-19 88.6
Msa0708770 AT5G01390.4 35.870 184 70 6 14 149 5 188 7.49e-22 94.0
Msa0708770 AT1G11040.1 37.500 136 83 1 215 350 291 424 4.04e-20 92.4
Msa0708770 AT1G44160.1 32.593 135 89 1 224 358 221 353 1.71e-17 84.0
Msa0708770 AT5G01390.3 40.741 108 43 3 14 100 5 112 9.72e-17 79.7
Msa0708770 AT2G21510.5 44.330 97 45 3 12 104 5 96 1.79e-15 77.4
Msa0708770 AT2G21510.3 44.330 97 45 3 12 104 5 96 2.50e-15 77.4
Msa0708770 AT2G21510.1 44.330 97 45 3 12 104 5 96 2.50e-15 77.4
Msa0708770 AT2G21510.2 44.330 97 45 3 12 104 5 96 2.57e-15 77.4
Msa0708770 AT2G21510.4 44.330 97 45 3 12 104 5 96 2.57e-15 77.4
Msa0708770 AT5G05750.1 41.748 103 46 3 14 102 115 217 4.26e-14 72.8
Msa0708770 AT4G39150.3 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0708770 AT4G39150.1 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0708770 AT4G39150.2 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0708770 AT1G59980.2 47.059 68 32 1 15 78 25 92 4.24e-13 70.5
Msa0708770 AT1G59980.1 47.059 68 32 1 15 78 25 92 6.38e-13 70.5
Msa0708770 AT5G03160.1 50.000 94 40 4 4 92 361 452 1.18e-12 69.7
Msa0708770 AT1G68370.1 51.471 68 29 1 15 78 19 86 1.25e-12 69.7
Msa0708770 AT3G57340.1 42.424 99 42 3 14 97 114 212 1.45e-12 68.9
Msa0708770 AT3G57340.2 42.424 99 42 3 14 97 114 212 1.45e-12 68.9
Msa0708770 AT3G08970.1 47.297 74 36 1 15 85 29 102 1.74e-12 69.7
Msa0708770 AT3G08970.2 47.297 74 36 1 15 85 29 102 1.74e-12 69.7
Msa0708770 AT1G24120.1 37.255 102 50 4 15 105 22 120 3.95e-12 68.2
Msa0708770 AT3G12170.2 46.479 71 34 1 8 74 42 112 1.25e-11 65.5
Msa0708770 AT3G12170.1 31.707 164 84 6 8 166 6 146 1.26e-11 65.1
Msa0708770 AT1G21080.3 39.175 97 49 3 12 104 5 95 2.07e-11 65.9
Msa0708770 AT1G21080.1 39.175 97 49 3 12 104 5 95 2.19e-11 65.5
Msa0708770 AT2G35720.1 38.824 85 45 1 5 82 7 91 2.76e-11 65.9
Msa0708770 AT1G74250.1 44.928 69 29 2 14 73 12 80 3.79e-11 65.5
Msa0708770 AT5G49060.2 46.269 67 33 1 11 74 97 163 4.00e-11 64.7
Msa0708770 AT5G49060.1 46.269 67 33 1 11 74 97 163 4.00e-11 64.7
Msa0708770 AT1G76700.1 39.394 99 51 3 12 106 5 98 6.20e-11 64.3
Msa0708770 AT4G10130.2 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0708770 AT4G10130.4 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0708770 AT4G10130.1 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0708770 AT4G10130.3 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0708770 AT4G10130.5 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0708770 AT1G56300.1 41.176 68 32 1 12 71 12 79 7.86e-11 60.8
Msa0708770 AT5G06910.1 45.714 70 34 1 9 74 25 94 9.22e-11 62.8

Find 141 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAAAGCTTGAAAATT+AGG 0.093537 4_4:-82496449 Msa0708770:intron
TTCGGTGGAGGTGGATTTCC+TGG 0.267655 4_4:-82495459 Msa0708770:intron
TTCACAAAGCTTGAAAATTA+GGG 0.287664 4_4:-82496448 Msa0708770:intron
TCACCCCTTGAAGGTCTCTT+TGG 0.287702 4_4:-82495287 Msa0708770:CDS
CCAATTTGCGCTGACTCATT+TGG 0.293928 4_4:-82494618 Msa0708770:CDS
GGAATGGGTGGTGGCGGTGG+TGG 0.294243 4_4:-82495513 Msa0708770:CDS
TCAGGATCACCACCCTTATC+AGG 0.295880 4_4:+82496260 None:intergenic
CCGTATAAAGATATCCTATA+TGG 0.308033 4_4:-82494019 Msa0708770:three_prime_UTR
CCAAATGAGTCAGCGCAAAT+TGG 0.320936 4_4:+82494618 None:intergenic
TTGATCAGACACCACTGTTT+TGG 0.322724 4_4:+82494074 None:intergenic
TTCACCCTTTCGTTTGAACT+TGG 0.337178 4_4:+82494691 None:intergenic
GAAGATATCTATGAGGCATT+TGG 0.338997 4_4:-82495049 Msa0708770:CDS
GAAGGAATGGGTGGTGGCGG+TGG 0.340853 4_4:-82495516 Msa0708770:CDS
ACTAAATACTATGAGATTCT+TGG 0.343941 4_4:-82496350 Msa0708770:CDS
AACTGAGATACAGGCCTTGC+TGG 0.344090 4_4:+82494298 None:intergenic
CCCCTTGAAGGTCTCTTTGG+AGG 0.348117 4_4:-82495284 Msa0708770:CDS
TTGCAGCCGGACACAGTTAC+TGG 0.350028 4_4:-82494749 Msa0708770:intron
CACCACCCATTCCTTCCTTA+AGG 0.353366 4_4:+82495523 None:intergenic
CCATATAGGATATCTTTATA+CGG 0.354474 4_4:+82494019 None:intergenic
TTAGTGTTATCACTCTTCTT+AGG 0.362823 4_4:+82496368 None:intergenic
TGTGCTGGTTGTCAAGGTTC+TGG 0.368082 4_4:-82495075 Msa0708770:CDS
GAGTTTGGAGATGTCCTTCA+TGG 0.373998 4_4:-82494117 Msa0708770:three_prime_UTR
TATTATCCTATGATTTAGAT+AGG 0.376273 4_4:-82493893 Msa0708770:three_prime_UTR
TCTTGATCAGGTCAAGGCTT+TGG 0.379949 4_4:-82494332 Msa0708770:CDS
TTGCATTTGCTGAATCATAT+TGG 0.385235 4_4:+82495023 None:intergenic
AAAGCTTGAAAATTAGGGTT+AGG 0.389474 4_4:-82496443 Msa0708770:intron
ACCACCCATTCCTTCCTTAA+GGG 0.391516 4_4:+82495524 None:intergenic
TACGCTTTCTTCAAATCATC+GGG 0.394989 4_4:+82496308 None:intergenic
CACCCTTTCGTTTGAACTTG+GGG 0.398325 4_4:+82494693 None:intergenic
TCACCCTTTCGTTTGAACTT+GGG 0.404206 4_4:+82494692 None:intergenic
AAGATATCTATGAGGCATTT+GGG 0.411623 4_4:-82495048 Msa0708770:CDS
ATGAAAGGCCTCTGGTACAT+TGG 0.411721 4_4:+82494406 None:intergenic
TTGTGAAATTTGAAGATGTT+TGG 0.412460 4_4:-82496398 Msa0708770:exon
TGAGTCTCTGTCTCTTGATC+AGG 0.415051 4_4:-82494344 Msa0708770:CDS
GTGCTCAGCAGTAATGAGTT+TGG 0.416716 4_4:-82494132 Msa0708770:exon
AATTATAAAATTAACCATAT+AGG 0.417706 4_4:+82494005 None:intergenic
ACATCCTTGCAATGAATGTA+AGG 0.418761 4_4:-82495001 Msa0708770:CDS
CTTTGGAGGATCTTTACCTT+GGG 0.422824 4_4:-82495270 Msa0708770:CDS
AGAGCTTGCACAAGCTTATG+AGG 0.426543 4_4:-82495598 Msa0708770:CDS
ATGTCTGTCAACTGAGATAC+AGG 0.432545 4_4:+82494289 None:intergenic
AAAGATGAAAAGATGTCAAA+TGG 0.433471 4_4:+82495483 None:intergenic
CCTTCTCTCCCTTGCACTGT+GGG 0.438316 4_4:+82494944 None:intergenic
TCATATACCTCACGCTTCTC+AGG 0.445588 4_4:+82495564 None:intergenic
TCCCTCACTGAGGCTTTATG+TGG 0.451630 4_4:-82494644 Msa0708770:CDS
TGCAATGAATGTAAGGGTAC+TGG 0.456888 4_4:-82494994 Msa0708770:CDS
TCATCTTTCTTCGGTGGAGG+TGG 0.461355 4_4:-82495468 Msa0708770:CDS
GTATGGTGAAGATGCCCTTA+AGG 0.462316 4_4:-82495538 Msa0708770:CDS
GAGATAGTGAATGATTATCT+TGG 0.463868 4_4:-82493782 None:intergenic
GGTTTGATTTAATCAATAGT+TGG 0.465413 4_4:+82494588 None:intergenic
ATACGCTTTCTTCAAATCAT+CGG 0.468985 4_4:+82496307 None:intergenic
CGTGAGGTATATGATCAGTA+TGG 0.471347 4_4:-82495555 Msa0708770:CDS
TCTTTGGAGGATCTTTACCT+TGG 0.472410 4_4:-82495271 Msa0708770:CDS
GATTAAGAATCATCCTGATA+AGG 0.476346 4_4:-82496273 Msa0708770:CDS
GGATGTCGTTCACCCCTTGA+AGG 0.478745 4_4:-82495296 Msa0708770:CDS
AGATCGATGCCCACAGTGCA+AGG 0.481792 4_4:-82494953 Msa0708770:CDS
ATTAAGAATCATCCTGATAA+GGG 0.485570 4_4:-82496272 Msa0708770:CDS
TACAGGAAAGGATCAAAGTC+TGG 0.495336 4_4:-82495111 Msa0708770:intron
ACTAACCTAGGGGAAATAAT+CGG 0.496006 4_4:+82493843 None:intergenic
TGCCCTTCATGAAAGGCCTC+TGG 0.498481 4_4:+82494398 None:intergenic
CCAGGCTTGACAACTTCTCC+AGG 0.499453 4_4:+82494566 None:intergenic
ATATCTCCAGTAACTGTGTC+CGG 0.501009 4_4:+82494743 None:intergenic
GGTGCTTCGATGACCTGTGC+TGG 0.505989 4_4:-82495090 Msa0708770:CDS
GATAATAAAGCATGCAAGGA+AGG 0.508424 4_4:+82493909 None:intergenic
CTTGAAGTTCATGTTGAGAA+GGG 0.510818 4_4:-82494901 Msa0708770:CDS
GCATCGATCTTTGTCACTGA+TGG 0.515086 4_4:+82494966 None:intergenic
ATCCTCCAAAGAGACCTTCA+AGG 0.518323 4_4:+82495282 None:intergenic
AAGGAAGGAATGGGTGGTGG+CGG 0.520461 4_4:-82495519 Msa0708770:CDS
ATAATGAATTACACTAACCT+AGG 0.520955 4_4:+82493831 None:intergenic
TACAAGGCAATCAATGATGA+AGG 0.521364 4_4:-82494433 Msa0708770:CDS
TTTGTACCTATCTAAATCAT+AGG 0.526396 4_4:+82493887 None:intergenic
CACCCCAAGTTCAAACGAAA+GGG 0.528668 4_4:-82494695 Msa0708770:CDS
AGATGCCCTTAAGGAAGGAA+TGG 0.532671 4_4:-82495529 Msa0708770:CDS
TGATCAAGAGACAGAGACTC+AGG 0.533617 4_4:+82494346 None:intergenic
CCTGGAGAAGTTGTCAAGCC+TGG 0.534032 4_4:-82494566 Msa0708770:intron
GGTCAGAAGATTACCTTCCC+TGG 0.534089 4_4:-82494868 Msa0708770:CDS
TTCATCAGCTTCACCAGGGA+AGG 0.534704 4_4:+82494855 None:intergenic
TAACACATACCCCTTGCACT+TGG 0.540192 4_4:+82495207 None:intergenic
ACTTGAAGTTCATGTTGAGA+AGG 0.543623 4_4:-82494902 Msa0708770:CDS
TAATGAATTACACTAACCTA+GGG 0.544568 4_4:+82493832 None:intergenic
CTTAAGGAAGGAATGGGTGG+TGG 0.547958 4_4:-82495522 Msa0708770:CDS
TGTCTTGTGCTCCAAGTGCA+AGG 0.549478 4_4:-82495218 Msa0708770:CDS
TACCAGAGGCCTTTCATGAA+GGG 0.550239 4_4:-82494400 Msa0708770:CDS
TCTGTCTCTTGATCAGGTCA+AGG 0.553837 4_4:-82494338 Msa0708770:CDS
GATGCCCTTAAGGAAGGAAT+GGG 0.556456 4_4:-82495528 Msa0708770:CDS
CCCACAGTGCAAGGGAGAGA+AGG 0.557002 4_4:-82494944 Msa0708770:CDS
CGTCTTTGTACTTCAACAGA+AGG 0.558455 4_4:-82494720 Msa0708770:CDS
TTGAAGAAGAGACAAGAAGA+AGG 0.558667 4_4:-82494222 Msa0708770:CDS
TAAAGCTTGCCCTTCATGAA+AGG 0.559348 4_4:+82494391 None:intergenic
GTCTTGTGCTCCAAGTGCAA+GGG 0.560067 4_4:-82495217 Msa0708770:CDS
ACCGCTTCATCAGCTTCACC+AGG 0.560539 4_4:+82494850 None:intergenic
ATGACCTGTGCTGGTTGTCA+AGG 0.561075 4_4:-82495081 Msa0708770:CDS
GTTGAGAAGGGGATGCAGAA+TGG 0.563375 4_4:-82494889 Msa0708770:CDS
TGGTTACGCAGATTCATACA+AGG 0.564992 4_4:-82494449 Msa0708770:intron
ATAAAGCATGCAAGGAAGGA+AGG 0.565798 4_4:+82493913 None:intergenic
GTACCAGAGGCCTTTCATGA+AGG 0.566111 4_4:-82494401 Msa0708770:CDS
ATAGGATAATAAAGCATGCA+AGG 0.566575 4_4:+82493905 None:intergenic
ACTGAGATACAGGCCTTGCT+GGG 0.567469 4_4:+82494299 None:intergenic
GGTGAAGATGCCCTTAAGGA+AGG 0.569050 4_4:-82495534 Msa0708770:CDS
TCAGTTGACAGACATGGAGC+TGG 0.570239 4_4:-82494281 Msa0708770:CDS
GTGGTAGTAGCAGAGGAAGA+AGG 0.572018 4_4:-82495333 Msa0708770:CDS
ACAGGTGGTGGTAGTAGCAG+AGG 0.575959 4_4:-82495340 Msa0708770:intron
CCCTGGTGAAGCTGATGAAG+CGG 0.582550 4_4:-82494851 Msa0708770:intron
AGAACCTTGACAACCAGCAC+AGG 0.585983 4_4:+82495077 None:intergenic
TGGAGACAATTCTCCCAGCA+AGG 0.587524 4_4:-82494312 Msa0708770:CDS
ACACCCCAAGTTCAAACGAA+AGG 0.592574 4_4:-82494696 Msa0708770:CDS
TGTACTCTTTGAGCACCACC+AGG 0.594168 4_4:+82494157 None:intergenic
AAGCTAAAACAAATCCATGA+AGG 0.594957 4_4:+82494103 None:intergenic
TCCTCCAAAGAGACCTTCAA+GGG 0.596017 4_4:+82495283 None:intergenic
TTGAAGTTCATGTTGAGAAG+GGG 0.603095 4_4:-82494900 Msa0708770:CDS
GTACCCTTACATTCATTGCA+AGG 0.605875 4_4:+82494997 None:intergenic
AGCTTTGTTGGCCAAAACAG+TGG 0.607380 4_4:-82494085 Msa0708770:three_prime_UTR
TTTCACCGATTATTTCCCCT+AGG 0.611137 4_4:-82493848 Msa0708770:three_prime_UTR
CAGGCTTGACAACTTCTCCA+GGG 0.612975 4_4:+82494567 None:intergenic
TAAGGGTGGTGATCCTGAAA+AGG 0.613088 4_4:-82496255 Msa0708770:intron
TGTATCTCAGTTGACAGACA+TGG 0.616220 4_4:-82494287 Msa0708770:CDS
GCCCTTAAGGAAGGAATGGG+TGG 0.620209 4_4:-82495525 Msa0708770:CDS
GATGAGGATGATGAAATGCC+TGG 0.624859 4_4:-82494175 Msa0708770:CDS
AAGGCAGCAAGCTCAGCAAG+AGG 0.624868 4_4:-82494203 Msa0708770:CDS
ACCTTCTCTCCCTTGCACTG+TGG 0.630501 4_4:+82494943 None:intergenic
GATCGATGCCCACAGTGCAA+GGG 0.633588 4_4:-82494952 Msa0708770:CDS
AAAGCTTCTTAGATGTCCCA+AGG 0.634241 4_4:+82495254 None:intergenic
TCTTGTGCTCCAAGTGCAAG+GGG 0.635966 4_4:-82495216 Msa0708770:intron
CTATTGATTAAATCAAACCC+TGG 0.637960 4_4:-82494584 Msa0708770:CDS
AGAGGAAGAAGGCAGAGACG+TGG 0.638556 4_4:-82495322 Msa0708770:CDS
CATCCTTGCAATGAATGTAA+GGG 0.643345 4_4:-82495000 Msa0708770:CDS
AAGAAGGCAGAGACGTGGTG+AGG 0.643979 4_4:-82495317 Msa0708770:CDS
TCAGCAAGAGGCATATGATG+AGG 0.654664 4_4:-82494191 Msa0708770:CDS
ACCACATAAAGCCTCAGTGA+GGG 0.659830 4_4:+82494643 None:intergenic
CATGGAGCTGGATGAGTGTG+AGG 0.667513 4_4:-82494269 Msa0708770:CDS
GATCAAGAGACAGAGACTCA+GGG 0.667543 4_4:+82494347 None:intergenic
AAGGAATGCCAATGTACCAG+AGG 0.667557 4_4:-82494414 Msa0708770:CDS
CTGGTATGAAGATATCTATG+AGG 0.673684 4_4:-82495056 Msa0708770:CDS
GAGTGATCCTGAGAAGCGTG+AGG 0.676105 4_4:-82495571 Msa0708770:CDS
AAGAATCATCCTGATAAGGG+TGG 0.677133 4_4:-82496269 Msa0708770:CDS
CCGCTTCATCAGCTTCACCA+GGG 0.690995 4_4:+82494851 None:intergenic
GCTTTACATTCACTTCACAG+TGG 0.719754 4_4:-82494374 Msa0708770:CDS
CCTCCAAAGAGACCTTCAAG+GGG 0.727234 4_4:+82495284 None:intergenic
AACCACATAAAGCCTCAGTG+AGG 0.740190 4_4:+82494642 None:intergenic
AATGAATTACACTAACCTAG+GGG 0.742373 4_4:+82493833 None:intergenic
GCACACATTGTCCCTCACTG+AGG 0.763892 4_4:-82494654 Msa0708770:CDS
GAGGATGATGAAATGCCTGG+TGG 0.804018 4_4:-82494172 Msa0708770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGAGATTTTTAAATTA+AGG - chr4_4:82494252-82494271 Msa0708770:CDS 10.0%
!!! ATTTGAGATTTTTAAATTAA+GGG - chr4_4:82494253-82494272 Msa0708770:CDS 10.0%
!! AATTATAAAATTAACCATAT+AGG + chr4_4:82496283-82496302 None:intergenic 10.0%
!! TTATCTTTGAATTTGATTAT+TGG - chr4_4:82494063-82494082 Msa0708770:three_prime_UTR 15.0%
!!! TTTGAGATTTTTAAATTAAG+GGG - chr4_4:82494254-82494273 Msa0708770:CDS 15.0%
!!! AAAAGTGATTTTTTTACTAT+AGG - chr4_4:82494294-82494313 Msa0708770:CDS 15.0%
!! ATTGAATTATATAATTGTTG+TGG - chr4_4:82494454-82494473 Msa0708770:intron 15.0%
!! ATTAGTTAATTGATGAAATT+AGG - chr4_4:82494627-82494646 Msa0708770:CDS 15.0%
!! TTAGTTAATTGATGAAATTA+GGG - chr4_4:82494628-82494647 Msa0708770:CDS 15.0%
!!! CTTGTTTGTTATTTTAATTT+AGG - chr4_4:82494857-82494876 Msa0708770:CDS 15.0%
!!! TTATTTTAATTTAGGAGATT+AGG - chr4_4:82494865-82494884 Msa0708770:CDS 15.0%
!!! TATTTTAATTTAGGAGATTA+GGG - chr4_4:82494866-82494885 Msa0708770:CDS 15.0%
!!! AAAATTTTAAAGATACAATC+AGG - chr4_4:82496481-82496500 Msa0708770:intron 15.0%
!!! TAAAAAAAAACAACCTTTTC+AGG + chr4_4:82494046-82494065 None:intergenic 20.0%
!!! ATTGAATTTGATAATTGTTG+TGG - chr4_4:82494127-82494146 Msa0708770:exon 20.0%
!!! AATAGTGATTAGTTGAATTT+AGG - chr4_4:82494154-82494173 Msa0708770:CDS 20.0%
!!! ATAGTGATTAGTTGAATTTA+GGG - chr4_4:82494155-82494174 Msa0708770:CDS 20.0%
!!! ATTGTTAGTTGTATTGATAT+TGG - chr4_4:82494502-82494521 Msa0708770:intron 20.0%
!!! TTGTTAGTTGTATTGATATT+GGG - chr4_4:82494503-82494522 Msa0708770:intron 20.0%
!!! AATTGTGATTAGTTAGTTTT+AGG - chr4_4:82494547-82494566 Msa0708770:intron 20.0%
!!! ATTGTGATTAGTTAGTTTTA+GGG - chr4_4:82494548-82494567 Msa0708770:intron 20.0%
!! AAAAATTGCTTAATAAGAGA+GGG + chr4_4:82496351-82496370 None:intergenic 20.0%
!! AAAAAATTGCTTAATAAGAG+AGG + chr4_4:82496352-82496371 None:intergenic 20.0%
!! TATTATCCTATGATTTAGAT+AGG - chr4_4:82496392-82496411 Msa0708770:exon 20.0%
!! ATTCTTTTGTTAGATCTCAA+CGG + chr4_4:82493815-82493834 None:intergenic 25.0%
! ATTCACAAAGCTTGAAAATT+AGG - chr4_4:82493836-82493855 Msa0708770:three_prime_UTR 25.0%
! TTCACAAAGCTTGAAAATTA+GGG - chr4_4:82493837-82493856 Msa0708770:three_prime_UTR 25.0%
! TTGTGAAATTTGAAGATGTT+TGG - chr4_4:82493887-82493906 Msa0708770:three_prime_UTR 25.0%
! ACTAAATACTATGAGATTCT+TGG - chr4_4:82493935-82493954 Msa0708770:three_prime_UTR 25.0%
! ATTAAGAATCATCCTGATAA+GGG - chr4_4:82494013-82494032 Msa0708770:three_prime_UTR 25.0%
! GATTATTGGATTATCTGATT+TGG - chr4_4:82494077-82494096 Msa0708770:three_prime_UTR 25.0%
! TTGGTAGAATGATATTTGTT+AGG - chr4_4:82494208-82494227 Msa0708770:CDS 25.0%
!! TGGTAGAATGATATTTGTTA+GGG - chr4_4:82494209-82494228 Msa0708770:CDS 25.0%
!!! TTGGTATTGATTATGCAATT+AGG - chr4_4:82494376-82494395 Msa0708770:CDS 25.0%
! ATTGATTATGCAATTAGGAT+TGG - chr4_4:82494381-82494400 Msa0708770:CDS 25.0%
! ATTGGGTTATATAATTGTTG+TGG - chr4_4:82494520-82494539 Msa0708770:intron 25.0%
! AAAGATGAAAAGATGTCAAA+TGG + chr4_4:82494805-82494824 None:intergenic 25.0%
! ATCATATTGTATTTGTGAAC+AGG - chr4_4:82494927-82494946 Msa0708770:CDS 25.0%
!!! GTTTGTTGGTATTTTTCTAA+TGG - chr4_4:82495119-82495138 Msa0708770:intron 25.0%
!! GGTTTGATTTAATCAATAGT+TGG + chr4_4:82495700-82495719 None:intergenic 25.0%
!! TGCTGAAATTTTCAATCTTT+TGG - chr4_4:82495816-82495835 Msa0708770:intron 25.0%
! CCATATAGGATATCTTTATA+CGG + chr4_4:82496269-82496288 None:intergenic 25.0%
! TTTGTACCTATCTAAATCAT+AGG + chr4_4:82496401-82496420 None:intergenic 25.0%
! TAATGAATTACACTAACCTA+GGG + chr4_4:82496456-82496475 None:intergenic 25.0%
! ATAATGAATTACACTAACCT+AGG + chr4_4:82496457-82496476 None:intergenic 25.0%
AAAGCTTGAAAATTAGGGTT+AGG - chr4_4:82493842-82493861 Msa0708770:three_prime_UTR 30.0%
TTAGTGTTATCACTCTTCTT+AGG + chr4_4:82493920-82493939 None:intergenic 30.0%
ATACGCTTTCTTCAAATCAT+CGG + chr4_4:82493981-82494000 None:intergenic 30.0%
GATTAAGAATCATCCTGATA+AGG - chr4_4:82494012-82494031 Msa0708770:three_prime_UTR 30.0%
!! AGGGTTAGTTTGATTGAATT+AGG - chr4_4:82494174-82494193 Msa0708770:CDS 30.0%
!! GGGTTAGTTTGATTGAATTA+GGG - chr4_4:82494175-82494194 Msa0708770:CDS 30.0%
TTTGATTGAATTAGGGTTGT+GGG - chr4_4:82494182-82494201 Msa0708770:CDS 30.0%
TTGTTAGGGTTTATTTGTCA+TGG - chr4_4:82494223-82494242 Msa0708770:CDS 30.0%
AATATCAATCCCATTAGCTA+CGG + chr4_4:82494324-82494343 None:intergenic 30.0%
! TTACCCAATTGATTGTTGTT+AGG - chr4_4:82494349-82494368 Msa0708770:CDS 30.0%
ACACCTAACAACAATCAATT+GGG + chr4_4:82494355-82494374 None:intergenic 30.0%
TACACCTAACAACAATCAAT+TGG + chr4_4:82494356-82494375 None:intergenic 30.0%
! TTGATTGTTGTTAGGTGTAT+TGG - chr4_4:82494357-82494376 Msa0708770:CDS 30.0%
AAAATCAAACTACCATAACC+CGG + chr4_4:82494594-82494613 None:intergenic 30.0%
! GACATCTTTTCATCTTTCTT+CGG - chr4_4:82494808-82494827 Msa0708770:intron 30.0%
ATATTGTATTTGTGAACAGG+TGG - chr4_4:82494930-82494949 Msa0708770:CDS 30.0%
! TTGGTATTTTTCTAATGGCT+CGG - chr4_4:82495124-82495143 Msa0708770:intron 30.0%
!! TTGTTGATTGGTTTGATTAC+AGG - chr4_4:82495157-82495176 Msa0708770:intron 30.0%
! AAGATATCTATGAGGCATTT+GGG - chr4_4:82495237-82495256 Msa0708770:CDS 30.0%
! TTGCATTTGCTGAATCATAT+TGG + chr4_4:82495265-82495284 None:intergenic 30.0%
! CTATTGATTAAATCAAACCC+TGG - chr4_4:82495701-82495720 Msa0708770:intron 30.0%
ATGTGAATAATCTACATACC+AGG + chr4_4:82495740-82495759 None:intergenic 30.0%
GTATGTAGATTATTCACATC+TGG - chr4_4:82495741-82495760 Msa0708770:intron 30.0%
!!! GACTGAATTTTTTTCGTCTT+TGG - chr4_4:82495793-82495812 Msa0708770:intron 30.0%
AAGCTAAAACAAATCCATGA+AGG + chr4_4:82496185-82496204 None:intergenic 30.0%
!!! TGGATTTGTTTTAGCTTTGT+TGG - chr4_4:82496188-82496207 Msa0708770:intron 30.0%
CCGTATAAAGATATCCTATA+TGG - chr4_4:82496266-82496285 Msa0708770:CDS 30.0%
ATAGGATAATAAAGCATGCA+AGG + chr4_4:82496383-82496402 None:intergenic 30.0%
AATGAATTACACTAACCTAG+GGG + chr4_4:82496455-82496474 None:intergenic 30.0%
TACGCTTTCTTCAAATCATC+GGG + chr4_4:82493980-82493999 None:intergenic 35.0%
GTTTGATTGAATTAGGGTTG+TGG - chr4_4:82494181-82494200 Msa0708770:CDS 35.0%
ATATCAATCCCATTAGCTAC+GGG + chr4_4:82494323-82494342 None:intergenic 35.0%
AGGGTAGTGTGATTGAATTA+AGG - chr4_4:82494567-82494586 Msa0708770:CDS 35.0%
AAATCAAACTACCATAACCC+GGG + chr4_4:82494593-82494612 None:intergenic 35.0%
!! GGGTTATGGTAGTTTGATTT+TGG - chr4_4:82494593-82494612 Msa0708770:CDS 35.0%
! ATCTTTTCATCTTTCTTCGG+TGG - chr4_4:82494811-82494830 Msa0708770:intron 35.0%
AACAAACAAGAAACCATACC+AGG + chr4_4:82494847-82494866 None:intergenic 35.0%
GGAGTTCTTACATTGTTGAT+TGG - chr4_4:82495145-82495164 Msa0708770:intron 35.0%
!! GATTGGTTTGATTACAGGAA+AGG - chr4_4:82495162-82495181 Msa0708770:intron 35.0%
CTGGTATGAAGATATCTATG+AGG - chr4_4:82495229-82495248 Msa0708770:CDS 35.0%
! GAAGATATCTATGAGGCATT+TGG - chr4_4:82495236-82495255 Msa0708770:CDS 35.0%
ACATCCTTGCAATGAATGTA+AGG - chr4_4:82495284-82495303 Msa0708770:CDS 35.0%
CATCCTTGCAATGAATGTAA+GGG - chr4_4:82495285-82495304 Msa0708770:CDS 35.0%
ACTTGAAGTTCATGTTGAGA+AGG - chr4_4:82495383-82495402 Msa0708770:intron 35.0%
CTTGAAGTTCATGTTGAGAA+GGG - chr4_4:82495384-82495403 Msa0708770:intron 35.0%
TTGAAGTTCATGTTGAGAAG+GGG - chr4_4:82495385-82495404 Msa0708770:intron 35.0%
TACAAGGCAATCAATGATGA+AGG - chr4_4:82495852-82495871 Msa0708770:intron 35.0%
TTGAAGAAGAGACAAGAAGA+AGG - chr4_4:82496063-82496082 Msa0708770:intron 35.0%
! CTGCAATTTTGCAAACACAA+GGG + chr4_4:82496310-82496329 None:intergenic 35.0%
GATAATAAAGCATGCAAGGA+AGG + chr4_4:82496379-82496398 None:intergenic 35.0%
ACTAACCTAGGGGAAATAAT+CGG + chr4_4:82496445-82496464 None:intergenic 35.0%
AAGAATCATCCTGATAAGGG+TGG - chr4_4:82494016-82494035 Msa0708770:three_prime_UTR 40.0%
TATCAATCCCATTAGCTACG+GGG + chr4_4:82494322-82494341 None:intergenic 40.0%
AGTGTGATTGAATTAAGGCC+CGG - chr4_4:82494572-82494591 Msa0708770:CDS 40.0%
CGTGAGGTATATGATCAGTA+TGG - chr4_4:82494730-82494749 Msa0708770:CDS 40.0%
! TTTTCATCTTTCTTCGGTGG+AGG - chr4_4:82494814-82494833 Msa0708770:intron 40.0%
TTGTATTTGTGAACAGGTGG+TGG - chr4_4:82494933-82494952 Msa0708770:CDS 40.0%
! TCTTTGGAGGATCTTTACCT+TGG - chr4_4:82495014-82495033 Msa0708770:CDS 40.0%
! CTTTGGAGGATCTTTACCTT+GGG - chr4_4:82495015-82495034 Msa0708770:CDS 40.0%
AAAGCTTCTTAGATGTCCCA+AGG + chr4_4:82495034-82495053 None:intergenic 40.0%
AGGTCTATCTGCATGTTTGT+TGG - chr4_4:82495105-82495124 Msa0708770:CDS 40.0%
TACAGGAAAGGATCAAAGTC+TGG - chr4_4:82495174-82495193 Msa0708770:intron 40.0%
GTACCCTTACATTCATTGCA+AGG + chr4_4:82495291-82495310 None:intergenic 40.0%
! TGCAATGAATGTAAGGGTAC+TGG - chr4_4:82495291-82495310 Msa0708770:CDS 40.0%
! TACAGTTCCTTAGTTTGAGC+TGG - chr4_4:82495471-82495490 Msa0708770:CDS 40.0%
CAACAATCCAGCTCAAACTA+AGG + chr4_4:82495481-82495500 None:intergenic 40.0%
CTTATTGTTTGTCTTGCAGC+CGG - chr4_4:82495523-82495542 Msa0708770:CDS 40.0%
ATATCTCCAGTAACTGTGTC+CGG + chr4_4:82495545-82495564 None:intergenic 40.0%
CGTCTTTGTACTTCAACAGA+AGG - chr4_4:82495565-82495584 Msa0708770:CDS 40.0%
TCACCCTTTCGTTTGAACTT+GGG + chr4_4:82495596-82495615 None:intergenic 40.0%
TTCACCCTTTCGTTTGAACT+TGG + chr4_4:82495597-82495616 None:intergenic 40.0%
TGGTTACGCAGATTCATACA+AGG - chr4_4:82495836-82495855 Msa0708770:intron 40.0%
TAAAGCTTGCCCTTCATGAA+AGG + chr4_4:82495897-82495916 None:intergenic 40.0%
GCTTTACATTCACTTCACAG+TGG - chr4_4:82495911-82495930 Msa0708770:intron 40.0%
ATGTCTGTCAACTGAGATAC+AGG + chr4_4:82495999-82496018 None:intergenic 40.0%
TGTATCTCAGTTGACAGACA+TGG - chr4_4:82495998-82496017 Msa0708770:intron 40.0%
! TTGATCAGACACCACTGTTT+TGG + chr4_4:82496214-82496233 None:intergenic 40.0%
! GCTGCAATTTTGCAAACACA+AGG + chr4_4:82496311-82496330 None:intergenic 40.0%
ATAAAGCATGCAAGGAAGGA+AGG + chr4_4:82496375-82496394 None:intergenic 40.0%
! TTTCACCGATTATTTCCCCT+AGG - chr4_4:82496437-82496456 Msa0708770:five_prime_UTR 40.0%
TAAGGGTGGTGATCCTGAAA+AGG - chr4_4:82494030-82494049 Msa0708770:three_prime_UTR 45.0%
! GAATTAGGGTTGTGGGTTGT+TGG - chr4_4:82494189-82494208 Msa0708770:CDS 45.0%
TATAGGATCCCCGTAGCTAA+TGG - chr4_4:82494311-82494330 Msa0708770:CDS 45.0%
ATAGGATCCCCGTAGCTAAT+GGG - chr4_4:82494312-82494331 Msa0708770:CDS 45.0%
GTGTGATTGAATTAAGGCCC+GGG - chr4_4:82494573-82494592 Msa0708770:CDS 45.0%
TTGAATTAAGGCCCGGGTTA+TGG - chr4_4:82494579-82494598 Msa0708770:CDS 45.0%
AGAGCTTGCACAAGCTTATG+AGG - chr4_4:82494687-82494706 Msa0708770:CDS 45.0%
TCATATACCTCACGCTTCTC+AGG + chr4_4:82494724-82494743 None:intergenic 45.0%
GTATGGTGAAGATGCCCTTA+AGG - chr4_4:82494747-82494766 Msa0708770:intron 45.0%
AGATGCCCTTAAGGAAGGAA+TGG - chr4_4:82494756-82494775 Msa0708770:intron 45.0%
GATGCCCTTAAGGAAGGAAT+GGG - chr4_4:82494757-82494776 Msa0708770:intron 45.0%
ACCACCCATTCCTTCCTTAA+GGG + chr4_4:82494764-82494783 None:intergenic 45.0%
TCCTCCAAAGAGACCTTCAA+GGG + chr4_4:82495005-82495024 None:intergenic 45.0%
ATCCTCCAAAGAGACCTTCA+AGG + chr4_4:82495006-82495025 None:intergenic 45.0%
TAACACATACCCCTTGCACT+TGG + chr4_4:82495081-82495100 None:intergenic 45.0%
! AGTGCAAGGGGTATGTGTTA+TGG - chr4_4:82495081-82495100 Msa0708770:CDS 45.0%
! CAAGGGGTATGTGTTATGGA+AGG - chr4_4:82495085-82495104 Msa0708770:CDS 45.0%
! GCATCGATCTTTGTCACTGA+TGG + chr4_4:82495322-82495341 None:intergenic 45.0%
ACACCCCAAGTTCAAACGAA+AGG - chr4_4:82495589-82495608 Msa0708770:CDS 45.0%
CACCCCAAGTTCAAACGAAA+GGG - chr4_4:82495590-82495609 Msa0708770:CDS 45.0%
CACCCTTTCGTTTGAACTTG+GGG + chr4_4:82495595-82495614 None:intergenic 45.0%
ACCACATAAAGCCTCAGTGA+GGG + chr4_4:82495645-82495664 None:intergenic 45.0%
AACCACATAAAGCCTCAGTG+AGG + chr4_4:82495646-82495665 None:intergenic 45.0%
CCAAATGAGTCAGCGCAAAT+TGG + chr4_4:82495670-82495689 None:intergenic 45.0%
!! CCAATTTGCGCTGACTCATT+TGG - chr4_4:82495667-82495686 Msa0708770:intron 45.0%
AAGGAATGCCAATGTACCAG+AGG - chr4_4:82495871-82495890 Msa0708770:intron 45.0%
ATGAAAGGCCTCTGGTACAT+TGG + chr4_4:82495882-82495901 None:intergenic 45.0%
TACCAGAGGCCTTTCATGAA+GGG - chr4_4:82495885-82495904 Msa0708770:intron 45.0%
GATCAAGAGACAGAGACTCA+GGG + chr4_4:82495941-82495960 None:intergenic 45.0%
TGATCAAGAGACAGAGACTC+AGG + chr4_4:82495942-82495961 None:intergenic 45.0%
TGAGTCTCTGTCTCTTGATC+AGG - chr4_4:82495941-82495960 Msa0708770:intron 45.0%
TCTGTCTCTTGATCAGGTCA+AGG - chr4_4:82495947-82495966 Msa0708770:intron 45.0%
TCTTGATCAGGTCAAGGCTT+TGG - chr4_4:82495953-82495972 Msa0708770:intron 45.0%
! TCAGCAAGAGGCATATGATG+AGG - chr4_4:82496094-82496113 Msa0708770:intron 45.0%
GATGAGGATGATGAAATGCC+TGG - chr4_4:82496110-82496129 Msa0708770:intron 45.0%
GTGCTCAGCAGTAATGAGTT+TGG - chr4_4:82496153-82496172 Msa0708770:intron 45.0%
GAGTTTGGAGATGTCCTTCA+TGG - chr4_4:82496168-82496187 Msa0708770:intron 45.0%
!! AGCTTTGTTGGCCAAAACAG+TGG - chr4_4:82496200-82496219 Msa0708770:intron 45.0%
TCAGGATCACCACCCTTATC+AGG + chr4_4:82494028-82494047 None:intergenic 50.0%
GGTGAAGATGCCCTTAAGGA+AGG - chr4_4:82494751-82494770 Msa0708770:intron 50.0%
CACCACCCATTCCTTCCTTA+AGG + chr4_4:82494765-82494784 None:intergenic 50.0%
CTTAAGGAAGGAATGGGTGG+TGG - chr4_4:82494763-82494782 Msa0708770:intron 50.0%
TCATCTTTCTTCGGTGGAGG+TGG - chr4_4:82494817-82494836 Msa0708770:intron 50.0%
! TGGAGGTGGATTTCCTGGTA+TGG - chr4_4:82494831-82494850 Msa0708770:intron 50.0%
GTGGTAGTAGCAGAGGAAGA+AGG - chr4_4:82494952-82494971 Msa0708770:CDS 50.0%
TCACCCCTTGAAGGTCTCTT+TGG - chr4_4:82494998-82495017 Msa0708770:CDS 50.0%
CCTCCAAAGAGACCTTCAAG+GGG + chr4_4:82495004-82495023 None:intergenic 50.0%
TGTCTTGTGCTCCAAGTGCA+AGG - chr4_4:82495067-82495086 Msa0708770:CDS 50.0%
GTCTTGTGCTCCAAGTGCAA+GGG - chr4_4:82495068-82495087 Msa0708770:CDS 50.0%
TCTTGTGCTCCAAGTGCAAG+GGG - chr4_4:82495069-82495088 Msa0708770:CDS 50.0%
!! ATGACCTGTGCTGGTTGTCA+AGG - chr4_4:82495204-82495223 Msa0708770:intron 50.0%
AGAACCTTGACAACCAGCAC+AGG + chr4_4:82495211-82495230 None:intergenic 50.0%
!! TGTGCTGGTTGTCAAGGTTC+TGG - chr4_4:82495210-82495229 Msa0708770:intron 50.0%
GTTGAGAAGGGGATGCAGAA+TGG - chr4_4:82495396-82495415 Msa0708770:intron 50.0%
GGTCAGAAGATTACCTTCCC+TGG - chr4_4:82495417-82495436 Msa0708770:intron 50.0%
TTCATCAGCTTCACCAGGGA+AGG + chr4_4:82495433-82495452 None:intergenic 50.0%
!!! GCTGATGAAGCGGTTGGTTT+TGG - chr4_4:82495444-82495463 Msa0708770:intron 50.0%
! TCCCTCACTGAGGCTTTATG+TGG - chr4_4:82495641-82495660 Msa0708770:intron 50.0%
CAGGCTTGACAACTTCTCCA+GGG + chr4_4:82495721-82495740 None:intergenic 50.0%
GTACCAGAGGCCTTTCATGA+AGG - chr4_4:82495884-82495903 Msa0708770:intron 50.0%
TGGAGACAATTCTCCCAGCA+AGG - chr4_4:82495973-82495992 Msa0708770:intron 50.0%
ACTGAGATACAGGCCTTGCT+GGG + chr4_4:82495989-82496008 None:intergenic 50.0%
AACTGAGATACAGGCCTTGC+TGG + chr4_4:82495990-82496009 None:intergenic 50.0%
TCAGTTGACAGACATGGAGC+TGG - chr4_4:82496004-82496023 Msa0708770:intron 50.0%
GAGGATGATGAAATGCCTGG+TGG - chr4_4:82496113-82496132 Msa0708770:intron 50.0%
TGTACTCTTTGAGCACCACC+AGG + chr4_4:82496131-82496150 None:intergenic 50.0%
! GAGTGATCCTGAGAAGCGTG+AGG - chr4_4:82494714-82494733 Msa0708770:CDS 55.0%
GCCCTTAAGGAAGGAATGGG+TGG - chr4_4:82494760-82494779 Msa0708770:intron 55.0%
! AAGGAAGGAATGGGTGGTGG+CGG - chr4_4:82494766-82494785 Msa0708770:intron 55.0%
! TTCGGTGGAGGTGGATTTCC+TGG - chr4_4:82494826-82494845 Msa0708770:intron 55.0%
! ACAGGTGGTGGTAGTAGCAG+AGG - chr4_4:82494945-82494964 Msa0708770:CDS 55.0%
AGAGGAAGAAGGCAGAGACG+TGG - chr4_4:82494963-82494982 Msa0708770:CDS 55.0%
AAGAAGGCAGAGACGTGGTG+AGG - chr4_4:82494968-82494987 Msa0708770:CDS 55.0%
GGATGTCGTTCACCCCTTGA+AGG - chr4_4:82494989-82495008 Msa0708770:CDS 55.0%
CCCCTTGAAGGTCTCTTTGG+AGG - chr4_4:82495001-82495020 Msa0708770:CDS 55.0%
AGATCGATGCCCACAGTGCA+AGG - chr4_4:82495332-82495351 Msa0708770:CDS 55.0%
GATCGATGCCCACAGTGCAA+GGG - chr4_4:82495333-82495352 Msa0708770:CDS 55.0%
CCTTCTCTCCCTTGCACTGT+GGG + chr4_4:82495344-82495363 None:intergenic 55.0%
ACCTTCTCTCCCTTGCACTG+TGG + chr4_4:82495345-82495364 None:intergenic 55.0%
CCGCTTCATCAGCTTCACCA+GGG + chr4_4:82495437-82495456 None:intergenic 55.0%
CCCTGGTGAAGCTGATGAAG+CGG - chr4_4:82495434-82495453 Msa0708770:intron 55.0%
ACCGCTTCATCAGCTTCACC+AGG + chr4_4:82495438-82495457 None:intergenic 55.0%
GGTGAAGCTGATGAAGCGGT+TGG - chr4_4:82495438-82495457 Msa0708770:intron 55.0%
TTGCAGCCGGACACAGTTAC+TGG - chr4_4:82495536-82495555 Msa0708770:CDS 55.0%
GCACACATTGTCCCTCACTG+AGG - chr4_4:82495631-82495650 Msa0708770:intron 55.0%
CCAGGCTTGACAACTTCTCC+AGG + chr4_4:82495722-82495741 None:intergenic 55.0%
CCTGGAGAAGTTGTCAAGCC+TGG - chr4_4:82495719-82495738 Msa0708770:intron 55.0%
TGCCCTTCATGAAAGGCCTC+TGG + chr4_4:82495890-82495909 None:intergenic 55.0%
CATGGAGCTGGATGAGTGTG+AGG - chr4_4:82496016-82496035 Msa0708770:intron 55.0%
AAGGCAGCAAGCTCAGCAAG+AGG - chr4_4:82496082-82496101 Msa0708770:intron 55.0%
GGTGCTTCGATGACCTGTGC+TGG - chr4_4:82495195-82495214 Msa0708770:intron 60.0%
CCCACAGTGCAAGGGAGAGA+AGG - chr4_4:82495341-82495360 Msa0708770:intron 60.0%
!! GAAGGAATGGGTGGTGGCGG+TGG - chr4_4:82494769-82494788 Msa0708770:intron 65.0%
!! GGAATGGGTGGTGGCGGTGG+TGG - chr4_4:82494772-82494791 Msa0708770:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 82493783 82496524 82493783 ID=Msa0708770;Name=Msa0708770
chr4_4 mRNA 82493783 82496524 82493783 ID=Msa0708770-mRNA-1;Parent=Msa0708770;Name=Msa0708770-mRNA-1;_AED=0.11;_eAED=0.11;_QI=88|1|1|1|0.83|0.71|7|358|417
chr4_4 exon 82493783 82494460 82493783 ID=Msa0708770-mRNA-1:exon:27181;Parent=Msa0708770-mRNA-1
chr4_4 exon 82494567 82494765 82494567 ID=Msa0708770-mRNA-1:exon:27180;Parent=Msa0708770-mRNA-1
chr4_4 exon 82494852 82495128 82494852 ID=Msa0708770-mRNA-1:exon:27179;Parent=Msa0708770-mRNA-1
chr4_4 exon 82495217 82495358 82495217 ID=Msa0708770-mRNA-1:exon:27178;Parent=Msa0708770-mRNA-1
chr4_4 exon 82495460 82495625 82495460 ID=Msa0708770-mRNA-1:exon:27177;Parent=Msa0708770-mRNA-1
chr4_4 exon 82496256 82496461 82496256 ID=Msa0708770-mRNA-1:exon:27176;Parent=Msa0708770-mRNA-1
chr4_4 exon 82496493 82496524 82496493 ID=Msa0708770-mRNA-1:exon:27175;Parent=Msa0708770-mRNA-1
chr4_4 five_prime_UTR 82496493 82496524 82496493 ID=Msa0708770-mRNA-1:five_prime_utr;Parent=Msa0708770-mRNA-1
chr4_4 five_prime_UTR 82496406 82496461 82496406 ID=Msa0708770-mRNA-1:five_prime_utr;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82496256 82496405 82496256 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82495460 82495625 82495460 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82495217 82495358 82495217 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82494852 82495128 82494852 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82494567 82494765 82494567 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 CDS 82494141 82494460 82494141 ID=Msa0708770-mRNA-1:cds;Parent=Msa0708770-mRNA-1
chr4_4 three_prime_UTR 82493783 82494140 82493783 ID=Msa0708770-mRNA-1:three_prime_utr;Parent=Msa0708770-mRNA-1
Gene Sequence

>Msa0708770

ATGTTTGGAAGAGCGCCTAAGAAGAGTGATAACACTAAATACTATGAGATTCTTGGTGTTTCGAAGAATGCTTCTCCCGATGATTTGAAGAAAGCGTATAAAAAAGCTGCGATTAAGAATCATCCTGATAAGGGTGGTGATCCTGAAAAGTTCAAAGAGCTTGCACAAGCTTATGAGGTTTTGAGTGATCCTGAGAAGCGTGAGGTATATGATCAGTATGGTGAAGATGCCCTTAAGGAAGGAATGGGTGGTGGCGGTGGTGGTCACGATCCATTTGACATCTTTTCATCTTTCTTCGGTGGAGGTGGATTTCCTGGTGGTGGTAGTAGCAGAGGAAGAAGGCAGAGACGTGGTGAGGATGTCGTTCACCCCTTGAAGGTCTCTTTGGAGGATCTTTACCTTGGGACATCTAAGAAGCTTTCTCTTTCACGAAATGTCTTGTGCTCCAAGTGCAAGGGGAAAGGATCAAAGTCTGGTGCTTCGATGACCTGTGCTGGTTGTCAAGGTTCTGGTATGAAGATATCTATGAGGCATTTGGGTGCCAATATGATTCAGCAAATGCAACATCCTTGCAATGAATGTAAGGGTACTGGAGAGACCATCAGTGACAAAGATCGATGCCCACAGTGCAAGGGAGAGAAGGTTGTTCAACAGAAGAAAGTACTTGAAGTTCATGTTGAGAAGGGGATGCAGAATGGTCAGAAGATTACCTTCCCTGGTGAAGCTGATGAAGCGCCGGACACAGTTACTGGAGATATCGTCTTTGTACTTCAACAGAAGGAACACCCCAAGTTCAAACGAAAGGGTGAAGATCTTTTTGTAGAGCACACATTGTCCCTCACTGAGGCTTTATGTGGTTTCCAATTTGCGCTGACTCATTTGGATTCTCGCCAACTATTGATTAAATCAAACCCTGGAGAAGTTGTCAAGCCTGATTCATACAAGGCAATCAATGATGAAGGAATGCCAATGTACCAGAGGCCTTTCATGAAGGGCAAGCTTTACATTCACTTCACAGTGGAGTTCCCTGAGTCTCTGTCTCTTGATCAGGTCAAGGCTTTGGAGACAATTCTCCCAGCAAGGCCTGTATCTCAGTTGACAGACATGGAGCTGGATGAGTGTGAGGAGACAACACTTCATGATGTCAACATTGAAGAAGAGACAAGAAGAAGGCAGCAAGCTCAGCAAGAGGCATATGATGAGGATGATGAAATGCCTGGTGGTGCTCAAAGAGTACAATGTGCTCAGCAGTAA
Protein sequence

>Msa0708770

MFGRAPKKSDNTKYYEILGVSKNASPDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREVYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGGFPGGGSSRGRRQRRGEDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCKGKGSKSGASMTCAGCQGSGMKISMRHLGANMIQQMQHPCNECKGTGETISDKDRCPQCKGEKVVQQKKVLEVHVEKGMQNGQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKGEDLFVEHTLSLTEALCGFQFALTHLDSRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPESLSLDQVKALETILPARPVSQLTDMELDECEETTLHDVNIEEETRRRQQAQQEAYDEDDEMPGGAQRVQCAQQ*