Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0755160 | A0A072UNS2 | 98.095 | 105 | 2 | 0 | 1 | 105 | 1 | 105 | 4.06e-68 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032840 | Msa0755160 | -0.802524 | 5.384823e-49 | -8.615850e-47 |
| Msa0064350 | Msa0755160 | 0.804609 | 1.990207e-49 | -8.615850e-47 |
| Msa0073670 | Msa0755160 | 0.808082 | 3.687945e-50 | -8.615850e-47 |
| Msa0639120 | Msa0755160 | 0.809823 | 1.563851e-50 | -8.615850e-47 |
| Msa0687630 | Msa0755160 | 0.805885 | 1.075606e-49 | -8.615850e-47 |
| Msa0692840 | Msa0755160 | 0.815879 | 7.373789e-52 | -8.615850e-47 |
| Msa0755160 | Msa0755340 | 0.870384 | 1.519752e-66 | -8.615850e-47 |
| Msa0755160 | Msa0759030 | 0.808923 | 2.439999e-50 | -8.615850e-47 |
| Msa0755160 | Msa0797120 | 0.805459 | 1.321452e-49 | -8.615850e-47 |
| Msa0755160 | Msa0797750 | 0.811575 | 6.537630e-51 | -8.615850e-47 |
| Msa0755160 | Msa0937550 | 0.820988 | 5.128237e-53 | -8.615850e-47 |
| Msa0755160 | Msa0942730 | 0.800494 | 1.403961e-48 | -8.615850e-47 |
| Msa0755160 | Msa1041530 | 0.801898 | 7.244490e-49 | -8.615850e-47 |
| Msa0755160 | Msa1126510 | 0.812893 | 3.371498e-51 | -8.615850e-47 |
| Msa0755160 | Msa1134110 | 0.802357 | 5.828699e-49 | -8.615850e-47 |
| Msa0755160 | Msa1165870 | 0.800152 | 1.648183e-48 | -8.615850e-47 |
| Msa0755160 | Msa1365150 | 0.804041 | 2.613254e-49 | -8.615850e-47 |
| Msa0755160 | Msa1367880 | 0.808218 | 3.449516e-50 | -8.615850e-47 |
| Msa0755160 | Msa1404190 | 0.805617 | 1.224527e-49 | -8.615850e-47 |
| Msa0143080 | Msa0755160 | 0.800689 | 1.280744e-48 | -8.615850e-47 |
| Msa0236980 | Msa0755160 | 0.810500 | 1.117619e-50 | -8.615850e-47 |
| Msa0328210 | Msa0755160 | 0.811439 | 6.999117e-51 | -8.615850e-47 |
| Msa0552810 | Msa0755160 | 0.802484 | 5.488603e-49 | -8.615850e-47 |
| Msa0560470 | Msa0755160 | 0.827579 | 1.447287e-54 | -8.615850e-47 |
| Msa0581070 | Msa0755160 | 0.808491 | 3.017410e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0755160 | MtrunA17_Chr5g0400191 | 98.095 | 105 | 2 | 0 | 1 | 105 | 1 | 105 | 7.83e-72 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0755160 | AT3G59840.1 | 58.095 | 105 | 36 | 2 | 1 | 105 | 1 | 97 | 1.65e-34 | 114 |
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGATAAGGTTACTCTTGC+CGG | 0.293256 | 5_2:+8953343 | Msa0755160:CDS |
| GTCGTCGACGACTTTGCTTC+AGG | 0.338950 | 5_2:-8953181 | Msa0755150:intron |
| GGTTCAAGATCTTCAGAAAC+TGG | 0.373697 | 5_2:-8953373 | None:intergenic |
| TTCAGAAACTGGACAATAAC+CGG | 0.394776 | 5_2:-8953362 | None:intergenic |
| ATCCCTGGTGCCTCCGTGGT+TGG | 0.425976 | 5_2:-8953244 | None:intergenic |
| GGTTGCTGCGAGCAACGATA+AGG | 0.463185 | 5_2:+8953330 | Msa0755160:CDS |
| GTTTACTGTGGTGGCGGAAG+GGG | 0.469288 | 5_2:+8953146 | Msa0755160:CDS |
| AGATCTTGAACCTTGTCGTT+GGG | 0.475907 | 5_2:+8953384 | Msa0755160:CDS |
| AGCAAAGAATGGCGGTGCCT+CGG | 0.478420 | 5_2:+8953213 | Msa0755160:CDS |
| GGGTTTACTGTGGTGGCGGA+AGG | 0.492850 | 5_2:+8953144 | Msa0755160:CDS |
| ACTGAAACACTCGCGTTCAA+AGG | 0.493969 | 5_2:-8953292 | None:intergenic |
| AAACCAACCACGGAGGCACC+AGG | 0.494110 | 5_2:+8953241 | Msa0755160:CDS |
| TCCACTGCACCGTTCATCCC+TGG | 0.497065 | 5_2:-8953259 | None:intergenic |
| AAGATCTTGAACCTTGTCGT+TGG | 0.501854 | 5_2:+8953383 | Msa0755160:CDS |
| ACGGTGCAGTGGAGGTTAAA+CGG | 0.502142 | 5_2:+8953269 | Msa0755160:CDS |
| GGTTTACTGTGGTGGCGGAA+GGG | 0.516623 | 5_2:+8953145 | Msa0755160:CDS |
| ACGGAGAACGCAGCAAAGAA+TGG | 0.516724 | 5_2:+8953202 | Msa0755160:CDS |
| TAAGAATGGGTTTACTGTGG+TGG | 0.544385 | 5_2:+8953137 | None:intergenic |
| GAATGGGTTTACTGTGGTGG+CGG | 0.545194 | 5_2:+8953140 | None:intergenic |
| TTGTCGTTGGGAAATTCTGC+CGG | 0.548386 | 5_2:+8953396 | Msa0755160:CDS |
| GGCGCGTTTGACTCAACCGC+CGG | 0.563952 | 5_2:-8953415 | None:intergenic |
| GGCAGAATTTCCCAACGACA+AGG | 0.605086 | 5_2:-8953394 | None:intergenic |
| ACGGAGGCACCAGGGATGAA+CGG | 0.607376 | 5_2:+8953250 | Msa0755160:CDS |
| AGGGATGAACGGTGCAGTGG+AGG | 0.608706 | 5_2:+8953261 | Msa0755160:CDS |
| ACCAGGGATGAACGGTGCAG+TGG | 0.613584 | 5_2:+8953258 | Msa0755160:CDS |
| GTTCATCCCTGGTGCCTCCG+TGG | 0.614953 | 5_2:-8953248 | None:intergenic |
| CGTGGTTGGTTTCACCGCCG+AGG | 0.625680 | 5_2:-8953230 | None:intergenic |
| CAGAAAACAACGCGAAACTG+CGG | 0.626649 | 5_2:-8953436 | None:intergenic |
| AAAGAATGGCGGTGCCTCGG+CGG | 0.629398 | 5_2:+8953216 | Msa0755160:CDS |
| TGAAGCAAAGTCGTCGACGA+CGG | 0.647435 | 5_2:+8953183 | Msa0755160:CDS |
| CGTTAAGAATGGGTTTACTG+TGG | 0.661274 | 5_2:+8953134 | None:intergenic |
| TCGTTGGGAAATTCTGCCGG+CGG | 0.672246 | 5_2:+8953399 | Msa0755160:CDS |
| AACCAACCACGGAGGCACCA+GGG | 0.708881 | 5_2:+8953242 | Msa0755160:CDS |
| GAGAACGCAGCAAAGAATGG+CGG | 0.729371 | 5_2:+8953205 | Msa0755160:CDS |
| CTCGGCGGTGAAACCAACCA+CGG | 0.734856 | 5_2:+8953231 | Msa0755160:CDS |
| GGCGGTGAAACCAACCACGG+AGG | 0.756389 | 5_2:+8953234 | Msa0755160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTGTTTCAGTTTTCGAGTT+TGG | + | chr5_2:8953304-8953323 | Msa0755160:CDS | 35.0% |
| !!! | TTCAGTTTTCGAGTTTGGTT+CGG | + | chr5_2:8953309-8953328 | Msa0755160:CDS | 35.0% |
| TTCAGAAACTGGACAATAAC+CGG | - | chr5_2:8953365-8953384 | None:intergenic | 35.0% | |
| AACGATAAGGTTACTCTTGC+CGG | + | chr5_2:8953343-8953362 | Msa0755160:CDS | 40.0% | |
| GGTTCAAGATCTTCAGAAAC+TGG | - | chr5_2:8953376-8953395 | None:intergenic | 40.0% | |
| AAGATCTTGAACCTTGTCGT+TGG | + | chr5_2:8953383-8953402 | Msa0755160:CDS | 40.0% | |
| AGATCTTGAACCTTGTCGTT+GGG | + | chr5_2:8953384-8953403 | Msa0755160:CDS | 40.0% | |
| ACTGAAACACTCGCGTTCAA+AGG | - | chr5_2:8953295-8953314 | None:intergenic | 45.0% | |
| TTGTCGTTGGGAAATTCTGC+CGG | + | chr5_2:8953396-8953415 | Msa0755160:CDS | 45.0% | |
| CAGAAAACAACGCGAAACTG+CGG | - | chr5_2:8953439-8953458 | None:intergenic | 45.0% | |
| TGAAGCAAAGTCGTCGACGA+CGG | + | chr5_2:8953183-8953202 | Msa0755160:CDS | 50.0% | |
| ACGGAGAACGCAGCAAAGAA+TGG | + | chr5_2:8953202-8953221 | Msa0755160:CDS | 50.0% | |
| GAGAACGCAGCAAAGAATGG+CGG | + | chr5_2:8953205-8953224 | Msa0755160:CDS | 50.0% | |
| ACGGTGCAGTGGAGGTTAAA+CGG | + | chr5_2:8953269-8953288 | Msa0755160:CDS | 50.0% | |
| GGCAGAATTTCCCAACGACA+AGG | - | chr5_2:8953397-8953416 | None:intergenic | 50.0% | |
| !! | GGTTTACTGTGGTGGCGGAA+GGG | + | chr5_2:8953145-8953164 | Msa0755160:CDS | 55.0% |
| !! | GTTTACTGTGGTGGCGGAAG+GGG | + | chr5_2:8953146-8953165 | Msa0755160:CDS | 55.0% |
| GTCGTCGACGACTTTGCTTC+AGG | - | chr5_2:8953184-8953203 | Msa0755150:five_prime_UTR | 55.0% | |
| AGCAAAGAATGGCGGTGCCT+CGG | + | chr5_2:8953213-8953232 | Msa0755160:CDS | 55.0% | |
| GGTTGCTGCGAGCAACGATA+AGG | + | chr5_2:8953330-8953349 | Msa0755160:CDS | 55.0% | |
| TCGTTGGGAAATTCTGCCGG+CGG | + | chr5_2:8953399-8953418 | Msa0755160:CDS | 55.0% | |
| !! | GGGTTTACTGTGGTGGCGGA+AGG | + | chr5_2:8953144-8953163 | Msa0755160:CDS | 60.0% |
| AAAGAATGGCGGTGCCTCGG+CGG | + | chr5_2:8953216-8953235 | Msa0755160:CDS | 60.0% | |
| CTCGGCGGTGAAACCAACCA+CGG | + | chr5_2:8953231-8953250 | Msa0755160:CDS | 60.0% | |
| !! | AAACCAACCACGGAGGCACC+AGG | + | chr5_2:8953241-8953260 | Msa0755160:CDS | 60.0% |
| !! | AACCAACCACGGAGGCACCA+GGG | + | chr5_2:8953242-8953261 | Msa0755160:CDS | 60.0% |
| !! | ACGGAGGCACCAGGGATGAA+CGG | + | chr5_2:8953250-8953269 | Msa0755160:CDS | 60.0% |
| ACCAGGGATGAACGGTGCAG+TGG | + | chr5_2:8953258-8953277 | Msa0755160:CDS | 60.0% | |
| TCCACTGCACCGTTCATCCC+TGG | - | chr5_2:8953262-8953281 | None:intergenic | 60.0% | |
| AGGGATGAACGGTGCAGTGG+AGG | + | chr5_2:8953261-8953280 | Msa0755160:CDS | 60.0% | |
| !! | CGTGGTTGGTTTCACCGCCG+AGG | - | chr5_2:8953233-8953252 | None:intergenic | 65.0% |
| GGCGGTGAAACCAACCACGG+AGG | + | chr5_2:8953234-8953253 | Msa0755160:CDS | 65.0% | |
| ATCCCTGGTGCCTCCGTGGT+TGG | - | chr5_2:8953247-8953266 | None:intergenic | 65.0% | |
| GTTCATCCCTGGTGCCTCCG+TGG | - | chr5_2:8953251-8953270 | None:intergenic | 65.0% | |
| GGCGCGTTTGACTCAACCGC+CGG | - | chr5_2:8953418-8953437 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 8953142 | 8953459 | 8953142 | ID=Msa0755160;Name=Msa0755160 |
| chr5_2 | mRNA | 8953142 | 8953459 | 8953142 | ID=Msa0755160-mRNA-1;Parent=Msa0755160;Name=Msa0755160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|105 |
| chr5_2 | exon | 8953142 | 8953459 | 8953142 | ID=Msa0755160-mRNA-1:exon:4047;Parent=Msa0755160-mRNA-1 |
| chr5_2 | CDS | 8953142 | 8953459 | 8953142 | ID=Msa0755160-mRNA-1:cds;Parent=Msa0755160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
