AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0755340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755340 MtrunA17_Chr5g0400191 98.095 105 2 0 1 105 1 105 7.83e-72 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755340 AT3G59840.1 58.095 105 36 2 1 105 1 97 1.65e-34 114

Find 36 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGATAAGGTTACTCTTGC+CGG 0.293256 5_2:+9109824 Msa0755340:CDS
GTCGTCGACGACTTTGCTTC+AGG 0.338950 5_2:-9109662 Msa0755330:intron
GGTTCAAGATCTTCAGAAAC+TGG 0.373697 5_2:-9109854 None:intergenic
TTCAGAAACTGGACAATAAC+CGG 0.394776 5_2:-9109843 None:intergenic
ATCCCTGGCGCCTCCGTGGT+TGG 0.398567 5_2:-9109725 None:intergenic
AAACCAACCACGGAGGCGCC+AGG 0.442569 5_2:+9109722 Msa0755340:CDS
GGTTGCTGCGAGCAACGATA+AGG 0.463185 5_2:+9109811 Msa0755340:CDS
GTTTACTGTGGTGGCGGAAG+GGG 0.469288 5_2:+9109627 Msa0755340:CDS
AGATCTTGAACCTTGTCGTT+GGG 0.475907 5_2:+9109865 Msa0755340:CDS
AGCAAAGAATGGCGGTGCCT+CGG 0.478420 5_2:+9109694 Msa0755340:CDS
TCCACTGCACCGTTCATCCC+TGG 0.487594 5_2:-9109740 None:intergenic
ACTGAAACACTCGCGTTCAA+AGG 0.493969 5_2:-9109773 None:intergenic
GGGTTTACTGTGGTGGCGGA+AGG 0.496501 5_2:+9109625 Msa0755340:CDS
AAGATCTTGAACCTTGTCGT+TGG 0.501854 5_2:+9109864 Msa0755340:CDS
ACGGTGCAGTGGAGGTTAAA+CGG 0.502142 5_2:+9109750 Msa0755340:CDS
ACGGAGAACGCAGCAAAGAA+TGG 0.516724 5_2:+9109683 Msa0755340:CDS
GGTTTACTGTGGTGGCGGAA+GGG 0.524570 5_2:+9109626 Msa0755340:CDS
TTGTCGTTGGGAAATTCTGC+CGG 0.548386 5_2:+9109877 Msa0755340:CDS
TTAAGATGGGTTTACTGTGG+TGG 0.549227 5_2:+9109618 None:intergenic
GGCGCGTTTGACTCAACCGC+CGG 0.563952 5_2:-9109896 None:intergenic
AGATGGGTTTACTGTGGTGG+CGG 0.565325 5_2:+9109621 None:intergenic
ACGGAGGCGCCAGGGATGAA+CGG 0.590223 5_2:+9109731 Msa0755340:CDS
AGGGATGAACGGTGCAGTGG+AGG 0.592078 5_2:+9109742 Msa0755340:CDS
GGCAGAATTTCCCAACGACA+AGG 0.605086 5_2:-9109875 None:intergenic
GTTCATCCCTGGCGCCTCCG+TGG 0.608954 5_2:-9109729 None:intergenic
AACCAACCACGGAGGCGCCA+GGG 0.609462 5_2:+9109723 Msa0755340:CDS
GCCAGGGATGAACGGTGCAG+TGG 0.624288 5_2:+9109739 Msa0755340:CDS
CGTGGTTGGTTTCACCGCCG+AGG 0.625680 5_2:-9109711 None:intergenic
CAGAAAACAACGCGAAACTG+CGG 0.626649 5_2:-9109917 None:intergenic
AAAGAATGGCGGTGCCTCGG+CGG 0.629398 5_2:+9109697 Msa0755340:CDS
GCGTTAAGATGGGTTTACTG+TGG 0.641308 5_2:+9109615 None:intergenic
TGAAGCAAAGTCGTCGACGA+CGG 0.647435 5_2:+9109664 Msa0755340:CDS
TCGTTGGGAAATTCTGCCGG+CGG 0.672246 5_2:+9109880 Msa0755340:CDS
GAGAACGCAGCAAAGAATGG+CGG 0.729371 5_2:+9109686 Msa0755340:CDS
CTCGGCGGTGAAACCAACCA+CGG 0.734856 5_2:+9109712 Msa0755340:CDS
GGCGGTGAAACCAACCACGG+AGG 0.756389 5_2:+9109715 Msa0755340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTGTTTCAGTTTTCGAGTT+TGG + chr5_2:9109785-9109804 Msa0755340:CDS 35.0%
!!! TTCAGTTTTCGAGTTTGGTT+CGG + chr5_2:9109790-9109809 Msa0755340:CDS 35.0%
TTCAGAAACTGGACAATAAC+CGG - chr5_2:9109846-9109865 None:intergenic 35.0%
AACGATAAGGTTACTCTTGC+CGG + chr5_2:9109824-9109843 Msa0755340:CDS 40.0%
GGTTCAAGATCTTCAGAAAC+TGG - chr5_2:9109857-9109876 None:intergenic 40.0%
AAGATCTTGAACCTTGTCGT+TGG + chr5_2:9109864-9109883 Msa0755340:CDS 40.0%
AGATCTTGAACCTTGTCGTT+GGG + chr5_2:9109865-9109884 Msa0755340:CDS 40.0%
ACTGAAACACTCGCGTTCAA+AGG - chr5_2:9109776-9109795 None:intergenic 45.0%
TTGTCGTTGGGAAATTCTGC+CGG + chr5_2:9109877-9109896 Msa0755340:CDS 45.0%
CAGAAAACAACGCGAAACTG+CGG - chr5_2:9109920-9109939 None:intergenic 45.0%
TGAAGCAAAGTCGTCGACGA+CGG + chr5_2:9109664-9109683 Msa0755340:CDS 50.0%
ACGGAGAACGCAGCAAAGAA+TGG + chr5_2:9109683-9109702 Msa0755340:CDS 50.0%
GAGAACGCAGCAAAGAATGG+CGG + chr5_2:9109686-9109705 Msa0755340:CDS 50.0%
ACGGTGCAGTGGAGGTTAAA+CGG + chr5_2:9109750-9109769 Msa0755340:CDS 50.0%
GGCAGAATTTCCCAACGACA+AGG - chr5_2:9109878-9109897 None:intergenic 50.0%
!! GGTTTACTGTGGTGGCGGAA+GGG + chr5_2:9109626-9109645 Msa0755340:CDS 55.0%
!! GTTTACTGTGGTGGCGGAAG+GGG + chr5_2:9109627-9109646 Msa0755340:CDS 55.0%
GTCGTCGACGACTTTGCTTC+AGG - chr5_2:9109665-9109684 Msa0755330:five_prime_UTR 55.0%
AGCAAAGAATGGCGGTGCCT+CGG + chr5_2:9109694-9109713 Msa0755340:CDS 55.0%
GGTTGCTGCGAGCAACGATA+AGG + chr5_2:9109811-9109830 Msa0755340:CDS 55.0%
TCGTTGGGAAATTCTGCCGG+CGG + chr5_2:9109880-9109899 Msa0755340:CDS 55.0%
!! GGGTTTACTGTGGTGGCGGA+AGG + chr5_2:9109625-9109644 Msa0755340:CDS 60.0%
AAAGAATGGCGGTGCCTCGG+CGG + chr5_2:9109697-9109716 Msa0755340:CDS 60.0%
CTCGGCGGTGAAACCAACCA+CGG + chr5_2:9109712-9109731 Msa0755340:CDS 60.0%
TCCACTGCACCGTTCATCCC+TGG - chr5_2:9109743-9109762 None:intergenic 60.0%
AGGGATGAACGGTGCAGTGG+AGG + chr5_2:9109742-9109761 Msa0755340:CDS 60.0%
!! CGTGGTTGGTTTCACCGCCG+AGG - chr5_2:9109714-9109733 None:intergenic 65.0%
GGCGGTGAAACCAACCACGG+AGG + chr5_2:9109715-9109734 Msa0755340:CDS 65.0%
!! AAACCAACCACGGAGGCGCC+AGG + chr5_2:9109722-9109741 Msa0755340:CDS 65.0%
!! AACCAACCACGGAGGCGCCA+GGG + chr5_2:9109723-9109742 Msa0755340:CDS 65.0%
!! ACGGAGGCGCCAGGGATGAA+CGG + chr5_2:9109731-9109750 Msa0755340:CDS 65.0%
GCCAGGGATGAACGGTGCAG+TGG + chr5_2:9109739-9109758 Msa0755340:CDS 65.0%
GGCGCGTTTGACTCAACCGC+CGG - chr5_2:9109899-9109918 None:intergenic 65.0%
ATCCCTGGCGCCTCCGTGGT+TGG - chr5_2:9109728-9109747 None:intergenic 70.0%
GTTCATCCCTGGCGCCTCCG+TGG - chr5_2:9109732-9109751 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 9109623 9109940 9109623 ID=Msa0755340;Name=Msa0755340
chr5_2 mRNA 9109623 9109940 9109623 ID=Msa0755340-mRNA-1;Parent=Msa0755340;Name=Msa0755340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|105
chr5_2 exon 9109623 9109940 9109623 ID=Msa0755340-mRNA-1:exon:4221;Parent=Msa0755340-mRNA-1
chr5_2 CDS 9109623 9109940 9109623 ID=Msa0755340-mRNA-1:cds;Parent=Msa0755340-mRNA-1
Gene Sequence

>Msa0755340

ATGGGTTTACTGTGGTGGCGGAAGGGGAAGAATTCACAACCTGAAGCAAAGTCGTCGACGACGGAGAACGCAGCAAAGAATGGCGGTGCCTCGGCGGTGAAACCAACCACGGAGGCGCCAGGGATGAACGGTGCAGTGGAGGTTAAACGGCCTTTGAACGCGAGTGTTTCAGTTTTCGAGTTTGGTTCGGTTGCTGCGAGCAACGATAAGGTTACTCTTGCCGGTTATTGTCCAGTTTCTGAAGATCTTGAACCTTGTCGTTGGGAAATTCTGCCGGCGGTTGAGTCAAACGCGCCGCAGTTTCGCGTTGTTTTCTGA

Protein sequence

>Msa0755340

MGLLWWRKGKNSQPEAKSSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPLNASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPCRWEILPAVESNAPQFRVVF*