Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0072420 | Msa0813530 | 0.804633 | 1.967225e-49 | -8.615850e-47 |
| Msa0631170 | Msa0813530 | 0.802979 | 4.338102e-49 | -8.615850e-47 |
| Msa0692980 | Msa0813530 | 0.815728 | 7.967812e-52 | -8.615850e-47 |
| Msa0716690 | Msa0813530 | 0.816385 | 5.682533e-52 | -8.615850e-47 |
| Msa0813530 | Msa0829330 | 0.804930 | 1.705025e-49 | -8.615850e-47 |
| Msa0813530 | Msa0854000 | 0.897861 | 9.323161e-77 | -8.615850e-47 |
| Msa0813530 | Msa0963270 | 0.820163 | 7.929564e-53 | -8.615850e-47 |
| Msa0813530 | Msa0992510 | 0.803447 | 3.470299e-49 | -8.615850e-47 |
| Msa0813530 | Msa1031660 | 0.826159 | 3.160537e-54 | -8.615850e-47 |
| Msa0813530 | Msa1070720 | 0.824319 | 8.607981e-54 | -8.615850e-47 |
| Msa0813530 | Msa1130980 | 0.813364 | 2.657682e-51 | -8.615850e-47 |
| Msa0813530 | Msa1228040 | 0.814875 | 1.233162e-51 | -8.615850e-47 |
| Msa0813530 | Msa1279400 | 0.803101 | 4.093536e-49 | -8.615850e-47 |
| Msa0813530 | Msa1308220 | 0.812753 | 3.618083e-51 | -8.615850e-47 |
| Msa0813530 | Msa1327640 | 0.816800 | 4.586752e-52 | -8.615850e-47 |
| Msa0813530 | Msa1392980 | 0.803352 | 3.631475e-49 | -8.615850e-47 |
| Msa0813530 | Msa1418340 | 0.809220 | 2.107315e-50 | -8.615850e-47 |
| Msa0127800 | Msa0813530 | 0.812178 | 4.830775e-51 | -8.615850e-47 |
| Msa0140820 | Msa0813530 | 0.801284 | 9.684231e-49 | -8.615850e-47 |
| Msa0204960 | Msa0813530 | 0.819535 | 1.103459e-52 | -8.615850e-47 |
| Msa0223610 | Msa0813530 | 0.800340 | 1.508979e-48 | -8.615850e-47 |
| Msa0229230 | Msa0813530 | 0.814484 | 1.505041e-51 | -8.615850e-47 |
| Msa0249860 | Msa0813530 | 0.836940 | 6.969927e-57 | -8.615850e-47 |
| Msa0281530 | Msa0813530 | 0.805535 | 1.274030e-49 | -8.615850e-47 |
| Msa0542290 | Msa0813530 | 0.801869 | 7.344109e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAATTACCCTAAAATTAA+AGG | 0.163872 | 5_3:-44414619 | Msa0813530:intron |
| GGTAATTAATCATGATATTA+TGG | 0.197528 | 5_3:+44414632 | None:intergenic |
| ACAATTCAGTTTCCTTGATA+TGG | 0.243136 | 5_3:+44414879 | None:intergenic |
| ATAAATTAGTTCCATATTCA+TGG | 0.286376 | 5_3:+44414659 | None:intergenic |
| TGATATGGCACTATGTTACT+AGG | 0.393716 | 5_3:+44414894 | None:intergenic |
| TGTGACGGTGGTAGTATTAT+TGG | 0.411712 | 5_3:-44414462 | Msa0813530:CDS |
| GATGGTAACACCGAGAGAAT+TGG | 0.417561 | 5_3:+44414419 | None:intergenic |
| GGTGACGGTGGTGGTATTGA+TGG | 0.478331 | 5_3:+44414401 | None:intergenic |
| CATATCAGCTCGGCTCAGTT+CGG | 0.500673 | 5_3:+44414796 | None:intergenic |
| ACCTATTGTTAGTGTTAGTA+CGG | 0.504598 | 5_3:+44414484 | None:intergenic |
| GCCGTACTAACACTAACAAT+AGG | 0.510181 | 5_3:-44414485 | Msa0813530:CDS |
| GTGAATCTCGCCGTGATATG+TGG | 0.519465 | 5_3:-44414750 | Msa0813530:five_prime_UTR |
| AGTAACATAGTGCCATATCA+AGG | 0.537977 | 5_3:-44414891 | Msa0813530:five_prime_UTR |
| GCGGTGAATAGTAGTGGACT+TGG | 0.557889 | 5_3:+44414380 | None:intergenic |
| AACACCAGCTCCAATTCTCT+CGG | 0.568073 | 5_3:-44414429 | Msa0813530:CDS |
| GACGGTGGTAGTATTATTGG+TGG | 0.568631 | 5_3:-44414459 | Msa0813530:CDS |
| AGTGGACTTGGTGACGGTGG+TGG | 0.574244 | 5_3:+44414392 | None:intergenic |
| ATTGCAGCAGCGGTGACAGT+GGG | 0.601286 | 5_3:-44414341 | Msa0813530:CDS |
| AACACTAACAATAGGTGTGA+CGG | 0.607101 | 5_3:-44414477 | Msa0813530:CDS |
| TATTGCAGCAGCGGTGACAG+TGG | 0.608654 | 5_3:-44414342 | Msa0813530:CDS |
| ACAGCAGCGGTGAATAGTAG+TGG | 0.615069 | 5_3:+44414374 | None:intergenic |
| GAGAGAATTGGAGCTGGTGT+TGG | 0.623654 | 5_3:+44414431 | None:intergenic |
| AGTTGATATTATTGCAGCAG+CGG | 0.629514 | 5_3:-44414351 | Msa0813530:CDS |
| AATAGTAGTGGACTTGGTGA+CGG | 0.643866 | 5_3:+44414386 | None:intergenic |
| TAGTAATATATAGTTGACAG+AGG | 0.648516 | 5_3:-44414838 | Msa0813530:five_prime_UTR |
| AGACGCTGATCCACATATCA+CGG | 0.648827 | 5_3:+44414740 | None:intergenic |
| AAGTCAAGTTCATATCAGCT+CGG | 0.649442 | 5_3:+44414786 | None:intergenic |
| AACACCGAGAGAATTGGAGC+TGG | 0.649987 | 5_3:+44414425 | None:intergenic |
| AATAATATCAACTACAGCAG+CGG | 0.669859 | 5_3:+44414361 | None:intergenic |
| AGTAGTGGACTTGGTGACGG+TGG | 0.690950 | 5_3:+44414389 | None:intergenic |
| ACTAACAATAGGTGTGACGG+TGG | 0.726512 | 5_3:-44414474 | Msa0813530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTTACCTTTAATTTTA+GGG | + | chr5_3:44414625-44414644 | None:intergenic | 10.0% |
| !!! | TATTATTTACCTTTAATTTT+AGG | + | chr5_3:44414626-44414645 | None:intergenic | 10.0% |
| !! | ATTAATTACCCTAAAATTAA+AGG | - | chr5_3:44414614-44414633 | Msa0813530:intron | 15.0% |
| !!! | ATATTAATTTTTTTGACAAC+AGG | - | chr5_3:44414643-44414662 | Msa0813530:exon | 15.0% |
| !! | ATAAATTAGTTCCATATTCA+TGG | + | chr5_3:44414577-44414596 | None:intergenic | 20.0% |
| !! | GGTAATTAATCATGATATTA+TGG | + | chr5_3:44414604-44414623 | None:intergenic | 20.0% |
| ! | TAGTAATATATAGTTGACAG+AGG | - | chr5_3:44414395-44414414 | Msa0813530:CDS | 25.0% |
| ! | CTAAAAAATCACCATGAATA+TGG | - | chr5_3:44414563-44414582 | Msa0813530:intron | 25.0% |
| ACAATTCAGTTTCCTTGATA+TGG | + | chr5_3:44414357-44414376 | None:intergenic | 30.0% | |
| ACCTATTGTTAGTGTTAGTA+CGG | + | chr5_3:44414752-44414771 | None:intergenic | 30.0% | |
| AATAATATCAACTACAGCAG+CGG | + | chr5_3:44414875-44414894 | None:intergenic | 30.0% | |
| TGATATGGCACTATGTTACT+AGG | + | chr5_3:44414342-44414361 | None:intergenic | 35.0% | |
| AGTAACATAGTGCCATATCA+AGG | - | chr5_3:44414342-44414361 | Msa0813530:CDS | 35.0% | |
| AAGTCAAGTTCATATCAGCT+CGG | + | chr5_3:44414450-44414469 | None:intergenic | 35.0% | |
| AACACTAACAATAGGTGTGA+CGG | - | chr5_3:44414756-44414775 | Msa0813530:five_prime_UTR | 35.0% | |
| ! | AGTTGATATTATTGCAGCAG+CGG | - | chr5_3:44414882-44414901 | Msa0813530:five_prime_UTR | 35.0% |
| ATGAGAAAAAGAAACGGAAG+AGG | + | chr5_3:44414918-44414937 | None:intergenic | 35.0% | |
| GCCGTACTAACACTAACAAT+AGG | - | chr5_3:44414748-44414767 | Msa0813530:five_prime_UTR | 40.0% | |
| TGTGACGGTGGTAGTATTAT+TGG | - | chr5_3:44414771-44414790 | Msa0813530:five_prime_UTR | 40.0% | |
| !! | AATAGTAGTGGACTTGGTGA+CGG | + | chr5_3:44414850-44414869 | None:intergenic | 40.0% |
| AGACGCTGATCCACATATCA+CGG | + | chr5_3:44414496-44414515 | None:intergenic | 45.0% | |
| ACTAACAATAGGTGTGACGG+TGG | - | chr5_3:44414759-44414778 | Msa0813530:five_prime_UTR | 45.0% | |
| GACGGTGGTAGTATTATTGG+TGG | - | chr5_3:44414774-44414793 | Msa0813530:five_prime_UTR | 45.0% | |
| AACACCAGCTCCAATTCTCT+CGG | - | chr5_3:44414804-44414823 | Msa0813530:five_prime_UTR | 45.0% | |
| GATGGTAACACCGAGAGAAT+TGG | + | chr5_3:44414817-44414836 | None:intergenic | 45.0% | |
| CATATCAGCTCGGCTCAGTT+CGG | + | chr5_3:44414440-44414459 | None:intergenic | 50.0% | |
| GTGAATCTCGCCGTGATATG+TGG | - | chr5_3:44414483-44414502 | Msa0813530:CDS | 50.0% | |
| GAGAGAATTGGAGCTGGTGT+TGG | + | chr5_3:44414805-44414824 | None:intergenic | 50.0% | |
| AACACCGAGAGAATTGGAGC+TGG | + | chr5_3:44414811-44414830 | None:intergenic | 50.0% | |
| !! | GCGGTGAATAGTAGTGGACT+TGG | + | chr5_3:44414856-44414875 | None:intergenic | 50.0% |
| ACAGCAGCGGTGAATAGTAG+TGG | + | chr5_3:44414862-44414881 | None:intergenic | 50.0% | |
| ! | GGTGACGGTGGTGGTATTGA+TGG | + | chr5_3:44414835-44414854 | None:intergenic | 55.0% |
| !! | AGTAGTGGACTTGGTGACGG+TGG | + | chr5_3:44414847-44414866 | None:intergenic | 55.0% |
| TATTGCAGCAGCGGTGACAG+TGG | - | chr5_3:44414891-44414910 | Msa0813530:five_prime_UTR | 55.0% | |
| ATTGCAGCAGCGGTGACAGT+GGG | - | chr5_3:44414892-44414911 | Msa0813530:five_prime_UTR | 55.0% | |
| !! | AGTGGACTTGGTGACGGTGG+TGG | + | chr5_3:44414844-44414863 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 44414316 | 44414939 | 44414316 | ID=Msa0813530;Name=Msa0813530 |
| chr5_3 | mRNA | 44414316 | 44414939 | 44414316 | ID=Msa0813530-mRNA-1;Parent=Msa0813530;Name=Msa0813530-mRNA-1;_AED=0.29;_eAED=0.56;_QI=295|0|0.5|1|0|0|2|0|71 |
| chr5_3 | exon | 44414316 | 44414506 | 44414316 | ID=Msa0813530-mRNA-1:exon:13706;Parent=Msa0813530-mRNA-1 |
| chr5_3 | exon | 44414620 | 44414939 | 44414620 | ID=Msa0813530-mRNA-1:exon:13705;Parent=Msa0813530-mRNA-1 |
| chr5_3 | five_prime_UTR | 44414645 | 44414939 | 44414645 | ID=Msa0813530-mRNA-1:five_prime_utr;Parent=Msa0813530-mRNA-1 |
| chr5_3 | CDS | 44414620 | 44414644 | 44414620 | ID=Msa0813530-mRNA-1:cds;Parent=Msa0813530-mRNA-1 |
| chr5_3 | CDS | 44414316 | 44414506 | 44414316 | ID=Msa0813530-mRNA-1:cds;Parent=Msa0813530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
