AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0819380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819380 MtrunA17_Chr5g0438511 68.715 179 37 3 1 163 1 176 5.22e-43 140
Msa0819380 MtrunA17_Chr5g0438491 80.000 50 7 1 1 47 1 50 1.43e-13 64.7
Msa0819380 MtrunA17_Chr5g0438481 77.551 49 8 1 1 46 1 49 8.75e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTCCTCTGATGCCAAAA+AGG 0.230354 5_3:-55869762 Msa0819380:CDS
TCCTTTAAAATATATGAATT+AGG 0.232146 5_3:-55868723 Msa0819380:three_prime_UTR
ATGTCCACCACCACCATATT+TGG 0.302002 5_3:+55869131 Msa0819390:intron
ATTGTGGTAACCGCCATATT+TGG 0.317393 5_3:+55869329 Msa0819390:three_prime_UTR
GAAGTAAACGATGCCAAATA+TGG 0.333960 5_3:-55869342 Msa0819380:CDS
GAAGTAAACGATGCCAAATA+TGG 0.342429 5_3:-55869144 Msa0819380:CDS
GGTGGTGGTGGACATTACTA+TGG 0.428994 5_3:-55869123 Msa0819380:CDS
AATGGTGGTGGACATTACTA+TGG 0.429163 5_3:-55869288 Msa0819380:CDS
GGAGGGAAAGAGTGATATGT+AGG 0.433161 5_3:+55869940 None:intergenic
TGGTTCTTCTTGTGTCAGCT+AGG 0.433566 5_3:-55869796 Msa0819380:CDS
GAAGTGTGCTCTATATATAA+TGG 0.448305 5_3:+55869919 None:intergenic
GATATGTAGGGACCTTTACT+TGG 0.450596 5_3:+55869953 None:intergenic
AATCCTCATCCTAGGCCTCT+TGG 0.458683 5_3:-55869822 Msa0819380:CDS
GAACCATTGCCAAGAGGCCT+AGG 0.460802 5_3:+55869813 None:intergenic
GGTGGTTCTCACCATGGTGG+AGG 0.471933 5_3:-55869078 Msa0819380:CDS
GGTGGTTCTCACGGTGGAGG+TGG 0.475576 5_3:-55869264 Msa0819380:CDS
GGTTCTCACCATGGTGGAGG+TGG 0.480643 5_3:-55869075 Msa0819380:CDS
GGTGGTTCTCACCACGGTGG+AGG 0.484853 5_3:-55869099 Msa0819380:CDS
GGTGGTTCTCACCACGGTGG+AGG 0.484853 5_3:-55869246 Msa0819380:CDS
CATCCTAGGCCTCTTGGCAA+TGG 0.496291 5_3:-55869816 Msa0819380:CDS
GGAGGTGGTTCTCACGGTGG+AGG 0.512830 5_3:-55869267 Msa0819380:CDS
GGTTCTTCTTGTGTCAGCTA+GGG 0.514897 5_3:-55869795 Msa0819380:CDS
GGTTCTCACCACGGTGGAGG+TGG 0.520241 5_3:-55869096 Msa0819380:CDS
GGTTCTCACCACGGTGGAGG+TGG 0.520241 5_3:-55869243 Msa0819380:CDS
TACTATGGAGGTGGTTCTCA+CGG 0.525230 5_3:-55869273 Msa0819380:CDS
ATTCAGCATGTGAGCAGCAA+TGG 0.536032 5_3:+55869031 Msa0819390:CDS
TTGCCAAGAGGCCTAGGATG+AGG 0.538496 5_3:+55869819 None:intergenic
GGTAATTACCACAATGGTGG+TGG 0.547595 5_3:-55869300 Msa0819380:CDS
CATGTGAGCAGCAATGGCCG+TGG 0.551382 5_3:+55869037 Msa0819390:CDS
GGCGGTTACCACAATGGTGG+TGG 0.554658 5_3:-55869321 Msa0819380:CDS
GGTGGACATTACTATGGAGG+TGG 0.556814 5_3:-55869117 Msa0819380:CDS
GGTGGACATTACTATGGAGG+TGG 0.556814 5_3:-55869282 Msa0819380:CDS
CATATGAGCAGCAATGGCCG+TGG 0.561023 5_3:+55869205 Msa0819390:CDS
CTTTCTGAACTACCAAGTAA+AGG 0.572070 5_3:-55869965 Msa0819380:five_prime_UTR
TTTGAAAAGATATTGAACAA+TGG 0.593441 5_3:-55869858 Msa0819380:exon
GATGCCAAATATGGTGGTGG+TGG 0.593462 5_3:-55869135 Msa0819380:CDS
GTTTGTTGCCGTGCAAGCTG+AGG 0.597485 5_3:-55869178 Msa0819380:CDS
GGTTGCCATTACTATTGCCA+CGG 0.599690 5_3:-55869054 Msa0819380:CDS
AATGGCCGTGGCAATAGTAA+TGG 0.601894 5_3:+55869049 Msa0819390:CDS
AAATATGGCGGTTACCACAA+TGG 0.601953 5_3:-55869327 Msa0819380:CDS
ACTCGGCATATGAGCAGCAA+TGG 0.602148 5_3:+55869199 Msa0819390:CDS
AGCTTGCACGGCAACAAACT+CGG 0.602417 5_3:+55869182 Msa0819390:intron
GGTAATTACCACCACCATTG+TGG 0.604720 5_3:+55869313 Msa0819390:three_prime_UTR
GGTGGAGGTGGTTCTCACCA+TGG 0.610718 5_3:-55869084 Msa0819380:CDS
GAGGGAAAGAGTGATATGTA+GGG 0.612466 5_3:+55869941 None:intergenic
GGTTGCCATCACTATTGCCA+CGG 0.613013 5_3:-55869222 Msa0819380:CDS
GTGTGCTCTATATATAATGG+AGG 0.615419 5_3:+55869922 None:intergenic
AATGGCCGTGGCAATAGTGA+TGG 0.615442 5_3:+55869217 Msa0819390:exon
AACGATGCCAAATATGGTGG+TGG 0.628814 5_3:-55869138 Msa0819380:CDS
GGTGGAGGTGGTTCTCACCA+CGG 0.641021 5_3:-55869252 Msa0819380:CDS
AGTAATGTCCACCACCATTG+TGG 0.641701 5_3:+55869292 Msa0819390:three_prime_UTR
GGTGGTGGACATTACTATGG+AGG 0.647255 5_3:-55869120 Msa0819380:CDS
TATGGAGGTGGTTCTCACCA+CGG 0.648357 5_3:-55869105 Msa0819380:CDS
TGTGCTCTATATATAATGGA+GGG 0.654334 5_3:+55869923 None:intergenic
GGAGGTGGTTCTCACCATGG+TGG 0.655820 5_3:-55869081 Msa0819380:CDS
ATTTGTTGCCGTGCAAGCTG+AGG 0.673816 5_3:-55869010 Msa0819380:CDS
CAAGAAGAACCATTGCCAAG+AGG 0.674082 5_3:+55869807 None:intergenic
AATGGCAACCACCTCCACCA+TGG 0.681347 5_3:+55869067 Msa0819390:CDS
GGTGGTAATTACCACAATGG+TGG 0.685453 5_3:-55869303 Msa0819380:CDS
GGTGGTGGACATTACTATGG+AGG 0.685934 5_3:-55869285 Msa0819380:CDS
GTAAACGATGCCAAATATGG+CGG 0.688367 5_3:-55869339 Msa0819380:CDS
TATGGAGGTGGTTCTCACGG+TGG 0.695015 5_3:-55869270 Msa0819380:CDS
GGAGGTGGTTCTCACCACGG+TGG 0.700953 5_3:-55869249 Msa0819380:CDS
ATCACACTATGTATGTGTGA+TGG 0.703321 5_3:-55868805 Msa0819380:three_prime_UTR
TATGGCGGTTACCACAATGG+TGG 0.720585 5_3:-55869324 Msa0819380:CDS
GGTGGTGGTAATTACCACAA+TGG 0.736293 5_3:-55869306 Msa0819380:CDS
GTAAACGATGCCAAATATGG+TGG 0.739461 5_3:-55869141 Msa0819380:CDS
GGAGGTGGTTCTCACCACGG+TGG 0.747901 5_3:-55869102 Msa0819380:CDS
GGTGAGAACCACCTCCACCG+TGG 0.755651 5_3:+55869088 Msa0819390:CDS
GATGGCAACCACCTCCACCG+TGG 0.756569 5_3:+55869235 Msa0819390:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCCTTTAAAATATATGAATT+AGG - chr5_3:55869953-55869972 Msa0819380:five_prime_UTR 15.0%
!!! ACCTAATTCATATATTTTAA+AGG + chr5_3:55869957-55869976 None:intergenic 15.0%
!!! TCCTTTAAAATATATGAATT+AGG - chr5_3:55869953-55869972 Msa0819380:five_prime_UTR 15.0%
!!! ACCTAATTCATATATTTTAA+AGG + chr5_3:55869957-55869976 None:intergenic 15.0%
!!! ATCTTTTCAAATGTGATATT+TGG + chr5_3:55868809-55868828 None:intergenic 20.0%
!! TTTGAAAAGATATTGAACAA+TGG - chr5_3:55868818-55868837 Msa0819380:three_prime_UTR 20.0%
!! ATCTCTATAAATTATAAAGC+AGG + chr5_3:55869128-55869147 Msa0819390:intron 20.0%
!! CGTATGTATATAAAATGTAA+TGG - chr5_3:55869269-55869288 Msa0819380:CDS 20.0%
!!! ATCTTTTCAAATGTGATATT+TGG + chr5_3:55868809-55868828 None:intergenic 20.0%
!! TTTGAAAAGATATTGAACAA+TGG - chr5_3:55868818-55868837 Msa0819380:three_prime_UTR 20.0%
!! ATCTCTATAAATTATAAAGC+AGG + chr5_3:55869128-55869147 Msa0819390:intron 20.0%
!! CGTATGTATATAAAATGTAA+TGG - chr5_3:55869269-55869288 Msa0819380:CDS 20.0%
!! TTGAACAATGGATTCAAAAA+AGG - chr5_3:55868830-55868849 Msa0819380:three_prime_UTR 25.0%
!!! ACTGCTAGCTTTATAAAATA+AGG - chr5_3:55869175-55869194 Msa0819380:CDS 25.0%
! CATATATATTATGTTTGCAG+AGG - chr5_3:55869294-55869313 Msa0819380:CDS 25.0%
!!! GTTTGTGAGTTGATAAAAAA+AGG - chr5_3:55869769-55869788 Msa0819380:CDS 25.0%
!! TTGAACAATGGATTCAAAAA+AGG - chr5_3:55868830-55868849 Msa0819380:three_prime_UTR 25.0%
!!! ACTGCTAGCTTTATAAAATA+AGG - chr5_3:55869175-55869194 Msa0819380:CDS 25.0%
! CATATATATTATGTTTGCAG+AGG - chr5_3:55869294-55869313 Msa0819380:CDS 25.0%
!!! GTTTGTGAGTTGATAAAAAA+AGG - chr5_3:55869769-55869788 Msa0819380:CDS 25.0%
! TGTGCTCTATATATAATGGA+GGG + chr5_3:55868756-55868775 None:intergenic 30.0%
GAAGTGTGCTCTATATATAA+TGG + chr5_3:55868760-55868779 None:intergenic 30.0%
! TGTGCTCTATATATAATGGA+GGG + chr5_3:55868756-55868775 None:intergenic 30.0%
GAAGTGTGCTCTATATATAA+TGG + chr5_3:55868760-55868779 None:intergenic 30.0%
CTTTCTGAACTACCAAGTAA+AGG - chr5_3:55868711-55868730 Msa0819380:three_prime_UTR 35.0%
GTGTGCTCTATATATAATGG+AGG + chr5_3:55868757-55868776 None:intergenic 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5_3:55869334-55869353 Msa0819380:CDS 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5_3:55869334-55869353 Msa0819380:CDS 35.0%
ATCACACTATGTATGTGTGA+TGG - chr5_3:55869871-55869890 Msa0819380:five_prime_UTR 35.0%
CTTTCTGAACTACCAAGTAA+AGG - chr5_3:55868711-55868730 Msa0819380:three_prime_UTR 35.0%
GTGTGCTCTATATATAATGG+AGG + chr5_3:55868757-55868776 None:intergenic 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5_3:55869334-55869353 Msa0819380:CDS 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5_3:55869334-55869353 Msa0819380:CDS 35.0%
ATCACACTATGTATGTGTGA+TGG - chr5_3:55869871-55869890 Msa0819380:five_prime_UTR 35.0%
GATATGTAGGGACCTTTACT+TGG + chr5_3:55868726-55868745 None:intergenic 40.0%
GAGGGAAAGAGTGATATGTA+GGG + chr5_3:55868738-55868757 None:intergenic 40.0%
AAAAAGGCAATCCTCATCCT+AGG - chr5_3:55868846-55868865 Msa0819380:three_prime_UTR 40.0%
AACTTCCTCTGATGCCAAAA+AGG - chr5_3:55868914-55868933 Msa0819380:three_prime_UTR 40.0%
ACTTCCTCTGATGCCAAAAA+GGG - chr5_3:55868915-55868934 Msa0819380:three_prime_UTR 40.0%
GTAAACGATGCCAAATATGG+CGG - chr5_3:55869337-55869356 Msa0819380:CDS 40.0%
ATTGTGGTAACCGCCATATT+TGG + chr5_3:55869350-55869369 Msa0819390:three_prime_UTR 40.0%
AAATATGGCGGTTACCACAA+TGG - chr5_3:55869349-55869368 Msa0819380:CDS 40.0%
!! AATGGTGGTGGACATTACTA+TGG - chr5_3:55869388-55869407 Msa0819380:intron 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5_3:55869337-55869356 Msa0819380:CDS 40.0%
! GTGATGAGCTTTTGCAAAAG+TGG - chr5_3:55869893-55869912 Msa0819380:five_prime_UTR 40.0%
GATATGTAGGGACCTTTACT+TGG + chr5_3:55868726-55868745 None:intergenic 40.0%
GAGGGAAAGAGTGATATGTA+GGG + chr5_3:55868738-55868757 None:intergenic 40.0%
AAAAAGGCAATCCTCATCCT+AGG - chr5_3:55868846-55868865 Msa0819380:three_prime_UTR 40.0%
AACTTCCTCTGATGCCAAAA+AGG - chr5_3:55868914-55868933 Msa0819380:three_prime_UTR 40.0%
ACTTCCTCTGATGCCAAAAA+GGG - chr5_3:55868915-55868934 Msa0819380:three_prime_UTR 40.0%
GTAAACGATGCCAAATATGG+CGG - chr5_3:55869337-55869356 Msa0819380:CDS 40.0%
ATTGTGGTAACCGCCATATT+TGG + chr5_3:55869350-55869369 Msa0819390:three_prime_UTR 40.0%
AAATATGGCGGTTACCACAA+TGG - chr5_3:55869349-55869368 Msa0819380:CDS 40.0%
!! AATGGTGGTGGACATTACTA+TGG - chr5_3:55869388-55869407 Msa0819380:intron 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5_3:55869337-55869356 Msa0819380:CDS 40.0%
! GTGATGAGCTTTTGCAAAAG+TGG - chr5_3:55869893-55869912 Msa0819380:five_prime_UTR 40.0%
GGAGGGAAAGAGTGATATGT+AGG + chr5_3:55868739-55868758 None:intergenic 45.0%
CAAGAAGAACCATTGCCAAG+AGG + chr5_3:55868872-55868891 None:intergenic 45.0%
TGGTTCTTCTTGTGTCAGCT+AGG - chr5_3:55868880-55868899 Msa0819380:three_prime_UTR 45.0%
GGTTCTTCTTGTGTCAGCTA+GGG - chr5_3:55868881-55868900 Msa0819380:three_prime_UTR 45.0%
! AGAGTGGAACTCACCCTTTT+TGG + chr5_3:55868931-55868950 None:intergenic 45.0%
GGTAATTACCACCACCATTG+TGG + chr5_3:55869366-55869385 Msa0819390:three_prime_UTR 45.0%
! GGTGGTGGTAATTACCACAA+TGG - chr5_3:55869370-55869389 Msa0819380:intron 45.0%
GGTGGTAATTACCACAATGG+TGG - chr5_3:55869373-55869392 Msa0819380:intron 45.0%
GGTAATTACCACAATGGTGG+TGG - chr5_3:55869376-55869395 Msa0819380:intron 45.0%
AGTAATGTCCACCACCATTG+TGG + chr5_3:55869387-55869406 None:intergenic 45.0%
TACTATGGAGGTGGTTCTCA+CGG - chr5_3:55869403-55869422 Msa0819380:intron 45.0%
! AGTCTTTTCCTCAGCTTGCA+CGG + chr5_3:55869509-55869528 None:intergenic 45.0%
AACGATGCCAAATATGGTGG+TGG - chr5_3:55869538-55869557 Msa0819380:intron 45.0%
ATGTCCACCACCACCATATT+TGG + chr5_3:55869548-55869567 None:intergenic 45.0%
GGTTGCCATTACTATTGCCA+CGG - chr5_3:55869622-55869641 Msa0819380:intron 45.0%
AATGGCCGTGGCAATAGTAA+TGG + chr5_3:55869630-55869649 None:intergenic 45.0%
ATTCAGCATGTGAGCAGCAA+TGG + chr5_3:55869648-55869667 None:intergenic 45.0%
!! AGTTTTGTCCTCAGCTTGCA+CGG + chr5_3:55869677-55869696 None:intergenic 45.0%
GGAGGGAAAGAGTGATATGT+AGG + chr5_3:55868739-55868758 None:intergenic 45.0%
CAAGAAGAACCATTGCCAAG+AGG + chr5_3:55868872-55868891 None:intergenic 45.0%
TGGTTCTTCTTGTGTCAGCT+AGG - chr5_3:55868880-55868899 Msa0819380:three_prime_UTR 45.0%
GGTTCTTCTTGTGTCAGCTA+GGG - chr5_3:55868881-55868900 Msa0819380:three_prime_UTR 45.0%
! AGAGTGGAACTCACCCTTTT+TGG + chr5_3:55868931-55868950 None:intergenic 45.0%
GGTAATTACCACCACCATTG+TGG + chr5_3:55869366-55869385 Msa0819390:three_prime_UTR 45.0%
! GGTGGTGGTAATTACCACAA+TGG - chr5_3:55869370-55869389 Msa0819380:intron 45.0%
GGTGGTAATTACCACAATGG+TGG - chr5_3:55869373-55869392 Msa0819380:intron 45.0%
GGTAATTACCACAATGGTGG+TGG - chr5_3:55869376-55869395 Msa0819380:intron 45.0%
AGTAATGTCCACCACCATTG+TGG + chr5_3:55869387-55869406 None:intergenic 45.0%
TACTATGGAGGTGGTTCTCA+CGG - chr5_3:55869403-55869422 Msa0819380:intron 45.0%
! AGTCTTTTCCTCAGCTTGCA+CGG + chr5_3:55869509-55869528 None:intergenic 45.0%
AACGATGCCAAATATGGTGG+TGG - chr5_3:55869538-55869557 Msa0819380:intron 45.0%
ATGTCCACCACCACCATATT+TGG + chr5_3:55869548-55869567 None:intergenic 45.0%
GGTTGCCATTACTATTGCCA+CGG - chr5_3:55869622-55869641 Msa0819380:intron 45.0%
AATGGCCGTGGCAATAGTAA+TGG + chr5_3:55869630-55869649 None:intergenic 45.0%
ATTCAGCATGTGAGCAGCAA+TGG + chr5_3:55869648-55869667 None:intergenic 45.0%
!! AGTTTTGTCCTCAGCTTGCA+CGG + chr5_3:55869677-55869696 None:intergenic 45.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5_3:55868854-55868873 Msa0819380:three_prime_UTR 50.0%
!! CTCACCCTTTTTGGCATCAG+AGG + chr5_3:55868922-55868941 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+TGG + chr5_3:55868947-55868966 Msa0819390:CDS 50.0%
TATGGCGGTTACCACAATGG+TGG - chr5_3:55869352-55869371 Msa0819380:CDS 50.0%
!! GGTGGTGGACATTACTATGG+AGG - chr5_3:55869391-55869410 Msa0819380:intron 50.0%
GGTGGACATTACTATGGAGG+TGG - chr5_3:55869394-55869413 Msa0819380:intron 50.0%
GGTTGCCATCACTATTGCCA+CGG - chr5_3:55869454-55869473 Msa0819380:intron 50.0%
AATGGCCGTGGCAATAGTGA+TGG + chr5_3:55869462-55869481 None:intergenic 50.0%
ACTCGGCATATGAGCAGCAA+TGG + chr5_3:55869480-55869499 None:intergenic 50.0%
AGCTTGCACGGCAACAAACT+CGG + chr5_3:55869497-55869516 None:intergenic 50.0%
!! GATGCCAAATATGGTGGTGG+TGG - chr5_3:55869541-55869560 Msa0819380:intron 50.0%
!! GGTGGTGGTGGACATTACTA+TGG - chr5_3:55869553-55869572 Msa0819380:intron 50.0%
!! GGTGGTGGACATTACTATGG+AGG - chr5_3:55869391-55869410 Msa0819380:intron 50.0%
GGTGGACATTACTATGGAGG+TGG - chr5_3:55869394-55869413 Msa0819380:intron 50.0%
TATGGAGGTGGTTCTCACCA+CGG - chr5_3:55869571-55869590 Msa0819380:intron 50.0%
! ATTTGTTGCCGTGCAAGCTG+AGG - chr5_3:55869666-55869685 Msa0819380:intron 50.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5_3:55868854-55868873 Msa0819380:three_prime_UTR 50.0%
!! CTCACCCTTTTTGGCATCAG+AGG + chr5_3:55868922-55868941 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+TGG + chr5_3:55868947-55868966 Msa0819390:CDS 50.0%
TATGGCGGTTACCACAATGG+TGG - chr5_3:55869352-55869371 Msa0819380:CDS 50.0%
!! GGTGGTGGACATTACTATGG+AGG - chr5_3:55869391-55869410 Msa0819380:intron 50.0%
GGTGGACATTACTATGGAGG+TGG - chr5_3:55869394-55869413 Msa0819380:intron 50.0%
GGTTGCCATCACTATTGCCA+CGG - chr5_3:55869454-55869473 Msa0819380:intron 50.0%
AATGGCCGTGGCAATAGTGA+TGG + chr5_3:55869462-55869481 None:intergenic 50.0%
ACTCGGCATATGAGCAGCAA+TGG + chr5_3:55869480-55869499 None:intergenic 50.0%
AGCTTGCACGGCAACAAACT+CGG + chr5_3:55869497-55869516 None:intergenic 50.0%
!! GATGCCAAATATGGTGGTGG+TGG - chr5_3:55869541-55869560 Msa0819380:intron 50.0%
!! GGTGGTGGTGGACATTACTA+TGG - chr5_3:55869553-55869572 Msa0819380:intron 50.0%
!! GGTGGTGGACATTACTATGG+AGG - chr5_3:55869391-55869410 Msa0819380:intron 50.0%
GGTGGACATTACTATGGAGG+TGG - chr5_3:55869394-55869413 Msa0819380:intron 50.0%
TATGGAGGTGGTTCTCACCA+CGG - chr5_3:55869571-55869590 Msa0819380:intron 50.0%
! ATTTGTTGCCGTGCAAGCTG+AGG - chr5_3:55869666-55869685 Msa0819380:intron 50.0%
TTGCCAAGAGGCCTAGGATG+AGG + chr5_3:55868860-55868879 None:intergenic 55.0%
CATCCTAGGCCTCTTGGCAA+TGG - chr5_3:55868860-55868879 Msa0819380:three_prime_UTR 55.0%
GAACCATTGCCAAGAGGCCT+AGG + chr5_3:55868866-55868885 None:intergenic 55.0%
TATGGAGGTGGTTCTCACGG+TGG - chr5_3:55869406-55869425 Msa0819380:intron 55.0%
CATATGAGCAGCAATGGCCG+TGG + chr5_3:55869474-55869493 None:intergenic 55.0%
! GTTTGTTGCCGTGCAAGCTG+AGG - chr5_3:55869498-55869517 Msa0819380:intron 55.0%
AATGGCAACCACCTCCACCA+TGG + chr5_3:55869612-55869631 None:intergenic 55.0%
TTGCCAAGAGGCCTAGGATG+AGG + chr5_3:55868860-55868879 None:intergenic 55.0%
CATCCTAGGCCTCTTGGCAA+TGG - chr5_3:55868860-55868879 Msa0819380:three_prime_UTR 55.0%
GAACCATTGCCAAGAGGCCT+AGG + chr5_3:55868866-55868885 None:intergenic 55.0%
TATGGAGGTGGTTCTCACGG+TGG - chr5_3:55869406-55869425 Msa0819380:intron 55.0%
CATATGAGCAGCAATGGCCG+TGG + chr5_3:55869474-55869493 None:intergenic 55.0%
! GTTTGTTGCCGTGCAAGCTG+AGG - chr5_3:55869498-55869517 Msa0819380:intron 55.0%
AATGGCAACCACCTCCACCA+TGG + chr5_3:55869612-55869631 None:intergenic 55.0%
GGCGGTTACCACAATGGTGG+TGG - chr5_3:55869355-55869374 Msa0819380:CDS 60.0%
GGTGGAGGTGGTTCTCACCA+CGG - chr5_3:55869424-55869443 Msa0819380:intron 60.0%
GGTGGAGGTGGTTCTCACCA+TGG - chr5_3:55869424-55869443 Msa0819380:intron 60.0%
GGAGGTGGTTCTCACCATGG+TGG - chr5_3:55869595-55869614 Msa0819380:intron 60.0%
GGTGGTTCTCACCATGGTGG+AGG - chr5_3:55869598-55869617 Msa0819380:intron 60.0%
! GGTTCTCACCATGGTGGAGG+TGG - chr5_3:55869601-55869620 Msa0819380:intron 60.0%
CATGTGAGCAGCAATGGCCG+TGG + chr5_3:55869642-55869661 None:intergenic 60.0%
GGCGGTTACCACAATGGTGG+TGG - chr5_3:55869355-55869374 Msa0819380:CDS 60.0%
GGTGGAGGTGGTTCTCACCA+CGG - chr5_3:55869424-55869443 Msa0819380:intron 60.0%
GGTGGAGGTGGTTCTCACCA+TGG - chr5_3:55869424-55869443 Msa0819380:intron 60.0%
GGAGGTGGTTCTCACCATGG+TGG - chr5_3:55869595-55869614 Msa0819380:intron 60.0%
GGTGGTTCTCACCATGGTGG+AGG - chr5_3:55869598-55869617 Msa0819380:intron 60.0%
! GGTTCTCACCATGGTGGAGG+TGG - chr5_3:55869601-55869620 Msa0819380:intron 60.0%
CATGTGAGCAGCAATGGCCG+TGG + chr5_3:55869642-55869661 None:intergenic 60.0%
GGAGGTGGTTCTCACGGTGG+AGG - chr5_3:55869409-55869428 Msa0819380:intron 65.0%
GGTGGTTCTCACGGTGGAGG+TGG - chr5_3:55869412-55869431 Msa0819380:intron 65.0%
GGAGGTGGTTCTCACCACGG+TGG - chr5_3:55869427-55869446 Msa0819380:intron 65.0%
GGTGGTTCTCACCACGGTGG+AGG - chr5_3:55869430-55869449 Msa0819380:intron 65.0%
GGTTCTCACCACGGTGGAGG+TGG - chr5_3:55869433-55869452 Msa0819380:intron 65.0%
GATGGCAACCACCTCCACCG+TGG + chr5_3:55869444-55869463 None:intergenic 65.0%
GGAGGTGGTTCTCACCACGG+TGG - chr5_3:55869427-55869446 Msa0819380:intron 65.0%
GGTGGTTCTCACCACGGTGG+AGG - chr5_3:55869430-55869449 Msa0819380:intron 65.0%
GGTTCTCACCACGGTGGAGG+TGG - chr5_3:55869433-55869452 Msa0819380:intron 65.0%
GGTGAGAACCACCTCCACCG+TGG + chr5_3:55869423-55869442 None:intergenic 65.0%
GGAGGTGGTTCTCACGGTGG+AGG - chr5_3:55869409-55869428 Msa0819380:intron 65.0%
GGTGGTTCTCACGGTGGAGG+TGG - chr5_3:55869412-55869431 Msa0819380:intron 65.0%
GGAGGTGGTTCTCACCACGG+TGG - chr5_3:55869427-55869446 Msa0819380:intron 65.0%
GGTGGTTCTCACCACGGTGG+AGG - chr5_3:55869430-55869449 Msa0819380:intron 65.0%
GGTTCTCACCACGGTGGAGG+TGG - chr5_3:55869433-55869452 Msa0819380:intron 65.0%
GATGGCAACCACCTCCACCG+TGG + chr5_3:55869444-55869463 None:intergenic 65.0%
GGAGGTGGTTCTCACCACGG+TGG - chr5_3:55869427-55869446 Msa0819380:intron 65.0%
GGTGGTTCTCACCACGGTGG+AGG - chr5_3:55869430-55869449 Msa0819380:intron 65.0%
GGTTCTCACCACGGTGGAGG+TGG - chr5_3:55869433-55869452 Msa0819380:intron 65.0%
GGTGAGAACCACCTCCACCG+TGG + chr5_3:55869423-55869442 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 55868692 55870006 55868692 ID=Msa0819380;Name=Msa0819380
chr5_3 mRNA 55868692 55870006 55868692 ID=Msa0819380-mRNA-1;Parent=Msa0819380;Name=Msa0819380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=145|1|1|1|1|1|2|301|163
chr5_3 exon 55868692 55869384 55868692 ID=Msa0819380-mRNA-1:exon:16863;Parent=Msa0819380-mRNA-1
chr5_3 exon 55869762 55870006 55869762 ID=Msa0819380-mRNA-1:exon:16862;Parent=Msa0819380-mRNA-1
chr5_3 five_prime_UTR 55869862 55870006 55869862 ID=Msa0819380-mRNA-1:five_prime_utr;Parent=Msa0819380-mRNA-1
chr5_3 CDS 55869762 55869861 55869762 ID=Msa0819380-mRNA-1:cds;Parent=Msa0819380-mRNA-1
chr5_3 CDS 55868993 55869384 55868993 ID=Msa0819380-mRNA-1:cds;Parent=Msa0819380-mRNA-1
chr5_3 three_prime_UTR 55868692 55868992 55868692 ID=Msa0819380-mRNA-1:three_prime_utr;Parent=Msa0819380-mRNA-1
Gene Sequence

>Msa0819380

ATGGATTCAAAAAAGGCAATCCTCATCCTAGGCCTCTTGGCAATGGTTCTTCTTGTGTCAGCTAGGGACTTAACTGAAACTTCCTCTGATGCCAAAAAGGAGGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGCGGTTACCACAATGGTGGTGGTAATTACCACAATGGTGGTGGACATTACTATGGAGGTGGTTCTCACGGTGGAGGTGGTTCTCACCACGGTGGAGGTGGTTGCCATCACTATTGCCACGGCCATTGCTGCTCATATGCCGAGTTTGTTGCCGTGCAAGCTGAGGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGTGGTGGACATTACTATGGAGGTGGTTCTCACCACGGTGGAGGTGGTTCTCACCATGGTGGAGGTGGTTGCCATTACTATTGCCACGGCCATTGCTGCTCACATGCTGAATTTGTTGCCGTGCAAGCTGAGGACAAAACTCAGAACTGA
Protein sequence

>Msa0819380

MDSKKAILILGLLAMVLLVSARDLTETSSDAKKEVVEKTNEVNDAKYGGYHNGGGNYHNGGGHYYGGGSHGGGGSHHGGGGCHHYCHGHCCSYAEFVAVQAEEKTNEVNDAKYGGGGHYYGGGSHHGGGGSHHGGGGCHYYCHGHCCSHAEFVAVQAEDKTQN*