Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0607510 | Msa0859500 | 0.801356 | 9.360159e-49 | -8.615850e-47 |
| Msa0609970 | Msa0859500 | 0.809701 | 1.661789e-50 | -8.615850e-47 |
| Msa0622340 | Msa0859500 | 0.811169 | 8.008670e-51 | -8.615850e-47 |
| Msa0650620 | Msa0859500 | 0.804609 | 1.990207e-49 | -8.615850e-47 |
| Msa0692110 | Msa0859500 | 0.806018 | 1.008622e-49 | -8.615850e-47 |
| Msa0738840 | Msa0859500 | 0.826754 | 2.279941e-54 | -8.615850e-47 |
| Msa0738850 | Msa0859500 | 0.819226 | 1.298100e-52 | -8.615850e-47 |
| Msa0738860 | Msa0859500 | 0.833777 | 4.388376e-56 | -8.615850e-47 |
| Msa0738870 | Msa0859500 | 0.805138 | 1.542437e-49 | -8.615850e-47 |
| Msa0738880 | Msa0859500 | 0.815799 | 7.683041e-52 | -8.615850e-47 |
| Msa0738890 | Msa0859500 | 0.879182 | 1.535729e-69 | -8.615850e-47 |
| Msa0738900 | Msa0859500 | 0.957049 | 6.903569e-115 | -8.615850e-47 |
| Msa0783270 | Msa0859500 | 0.812138 | 4.930635e-51 | -8.615850e-47 |
| Msa0819320 | Msa0859500 | 0.802972 | 4.352275e-49 | -8.615850e-47 |
| Msa0819360 | Msa0859500 | 0.831524 | 1.589490e-55 | -8.615850e-47 |
| Msa0819370 | Msa0859500 | 0.814527 | 1.472138e-51 | -8.615850e-47 |
| Msa0819380 | Msa0859500 | 0.893210 | 7.768980e-75 | -8.615850e-47 |
| Msa0819390 | Msa0859500 | 0.956685 | 1.645820e-114 | -8.615850e-47 |
| Msa0168640 | Msa0859500 | -0.806066 | 9.854108e-50 | -8.615850e-47 |
| Msa0419640 | Msa0859500 | -0.826336 | 2.869411e-54 | -8.615850e-47 |
| Msa0426810 | Msa0859500 | 0.826511 | 2.605590e-54 | -8.615850e-47 |
| Msa0295580 | Msa0859500 | 0.827499 | 1.513020e-54 | -8.615850e-47 |
| Msa0859490 | Msa0859500 | 0.885464 | 7.953400e-72 | -8.615850e-47 |
| Msa0859500 | Msa0859520 | 0.813572 | 2.391910e-51 | -8.615850e-47 |
| Msa0859500 | Msa0859530 | 0.840134 | 1.044227e-57 | -8.615850e-47 |
| Msa0859500 | Msa0871890 | 0.805900 | 1.067850e-49 | -8.615850e-47 |
| Msa0859500 | Msa0922540 | 0.830379 | 3.035836e-55 | -8.615850e-47 |
| Msa0859500 | Msa0942730 | 0.807523 | 4.850140e-50 | -8.615850e-47 |
| Msa0859500 | Msa0958020 | 0.802069 | 6.681502e-49 | -8.615850e-47 |
| Msa0859500 | Msa0960150 | -0.815523 | 8.848836e-52 | -8.615850e-47 |
| Msa0859500 | Msa0996090 | 0.803937 | 2.746301e-49 | -8.615850e-47 |
| Msa0859500 | Msa1002040 | 0.815532 | 8.810125e-52 | -8.615850e-47 |
| Msa0859500 | Msa1218650 | 0.815612 | 8.456171e-52 | -8.615850e-47 |
| Msa0859500 | Msa1382410 | 0.812965 | 3.251013e-51 | -8.615850e-47 |
| Msa0859500 | Msa1390310 | 0.803319 | 3.690267e-49 | -8.615850e-47 |
| Msa0859500 | Msa1439230 | 0.806231 | 9.093648e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTGGTAACCGCCATATT+TGG | 0.317393 | 5_4:-57812745 | Msa0859500:CDS |
| GAAGTAAACGATGCCAAATA+TGG | 0.333960 | 5_4:+57812732 | Msa0859490:CDS |
| AATGGTGGTGGACATTACTA+TGG | 0.429163 | 5_4:+57812786 | Msa0859490:CDS |
| GGTGGTTCTCACCATGGTGG+AGG | 0.471933 | 5_4:+57812996 | Msa0859490:CDS |
| GGTGGTTCTCACGGTGGAGG+TGG | 0.475576 | 5_4:+57812810 | Msa0859490:CDS |
| GGTTCTCACCATGGTGGAGG+TGG | 0.480643 | 5_4:+57812999 | Msa0859490:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_4:+57812828 | Msa0859490:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_4:+57812975 | Msa0859490:CDS |
| GGAGGTGGTTCTCACGGTGG+AGG | 0.512830 | 5_4:+57812807 | Msa0859490:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_4:+57812831 | Msa0859490:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_4:+57812978 | Msa0859490:CDS |
| TACTATGGAGGTGGTTCTCA+CGG | 0.525230 | 5_4:+57812801 | Msa0859490:CDS |
| GGTAATTACCACAATGGTGG+TGG | 0.547595 | 5_4:+57812774 | Msa0859490:CDS |
| CATGTGAGCAGCAATGGCCG+TGG | 0.551382 | 5_4:-57813037 | None:intergenic |
| GGCGGTTACCACAATGGTGG+TGG | 0.554658 | 5_4:+57812753 | Msa0859490:CDS |
| GGTGGACATTACTATGGAGG+TGG | 0.556814 | 5_4:+57812792 | Msa0859490:CDS |
| CATATGAGCAGCAATGGCCG+TGG | 0.561023 | 5_4:-57812869 | Msa0859500:CDS |
| GGTTGCCATTACTATTGCCA+CGG | 0.599690 | 5_4:+57813020 | Msa0859490:CDS |
| AATGGCCGTGGCAATAGTAA+TGG | 0.601894 | 5_4:-57813025 | Msa0859500:CDS |
| AAATATGGCGGTTACCACAA+TGG | 0.601953 | 5_4:+57812747 | Msa0859490:CDS |
| ACTCGGCATATGAGCAGCAA+TGG | 0.602148 | 5_4:-57812875 | Msa0859500:CDS |
| AGCTTGCACGGCAACAAACT+CGG | 0.602417 | 5_4:-57812892 | Msa0859500:CDS |
| GGTAATTACCACCACCATTG+TGG | 0.604720 | 5_4:-57812761 | Msa0859500:CDS |
| GGTGGAGGTGGTTCTCACCA+TGG | 0.610718 | 5_4:+57812990 | Msa0859490:CDS |
| GGTTGCCATCACTATTGCCA+CGG | 0.613013 | 5_4:+57812852 | Msa0859490:CDS |
| AATGGCCGTGGCAATAGTGA+TGG | 0.615442 | 5_4:-57812857 | Msa0859500:CDS |
| GGTGGAGGTGGTTCTCACCA+CGG | 0.641021 | 5_4:+57812822 | Msa0859490:CDS |
| AGTAATGTCCACCACCATTG+TGG | 0.641701 | 5_4:-57812782 | Msa0859500:CDS |
| GGAGGTGGTTCTCACCATGG+TGG | 0.655820 | 5_4:+57812993 | Msa0859490:CDS |
| AATGGCAACCACCTCCACCA+TGG | 0.681347 | 5_4:-57813007 | Msa0859500:CDS |
| GGTGGTAATTACCACAATGG+TGG | 0.685453 | 5_4:+57812771 | Msa0859490:CDS |
| GGTGGTGGACATTACTATGG+AGG | 0.685934 | 5_4:+57812789 | Msa0859490:CDS |
| GTAAACGATGCCAAATATGG+CGG | 0.688367 | 5_4:+57812735 | Msa0859490:CDS |
| TATGGAGGTGGTTCTCACGG+TGG | 0.695015 | 5_4:+57812804 | Msa0859490:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.700953 | 5_4:+57812825 | Msa0859490:CDS |
| TATGGCGGTTACCACAATGG+TGG | 0.720585 | 5_4:+57812750 | Msa0859490:CDS |
| GGTGGTGGTAATTACCACAA+TGG | 0.736293 | 5_4:+57812768 | Msa0859490:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.747901 | 5_4:+57812972 | Msa0859490:CDS |
| GGTGAGAACCACCTCCACCG+TGG | 0.755651 | 5_4:-57812986 | Msa0859500:intron |
| GATGGCAACCACCTCCACCG+TGG | 0.756569 | 5_4:-57812839 | Msa0859500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGTAAACGATGCCAAATA+TGG | + | chr5_4:57812808-57812827 | Msa0859490:CDS | 35.0% | |
| GAAGTAAACGATGCCAAATA+TGG | + | chr5_4:57812808-57812827 | Msa0859490:CDS | 35.0% | |
| GTAAACGATGCCAAATATGG+TGG | + | chr5_4:57812805-57812824 | Msa0859490:CDS | 40.0% | |
| !! | AATGGTGGTGGACATTACTA+TGG | + | chr5_4:57812952-57812971 | Msa0859490:CDS | 40.0% |
| AAATATGGCGGTTACCACAA+TGG | + | chr5_4:57812991-57813010 | Msa0859490:CDS | 40.0% | |
| ATTGTGGTAACCGCCATATT+TGG | - | chr5_4:57812990-57813009 | Msa0859500:CDS | 40.0% | |
| GTAAACGATGCCAAATATGG+CGG | + | chr5_4:57812805-57812824 | Msa0859490:CDS | 40.0% | |
| GGTTGCCATTACTATTGCCA+CGG | + | chr5_4:57812718-57812737 | Msa0859490:CDS | 45.0% | |
| ATGTCCACCACCACCATATT+TGG | - | chr5_4:57812792-57812811 | Msa0859500:CDS | 45.0% | |
| AACGATGCCAAATATGGTGG+TGG | + | chr5_4:57812802-57812821 | Msa0859490:CDS | 45.0% | |
| ! | AGTCTTTTCCTCAGCTTGCA+CGG | - | chr5_4:57812831-57812850 | Msa0859500:CDS | 45.0% |
| TACTATGGAGGTGGTTCTCA+CGG | + | chr5_4:57812772-57812791 | Msa0859490:CDS | 45.0% | |
| AGTAATGTCCACCACCATTG+TGG | - | chr5_4:57812953-57812972 | Msa0859500:intron | 45.0% | |
| GGTAATTACCACAATGGTGG+TGG | + | chr5_4:57812964-57812983 | Msa0859490:CDS | 45.0% | |
| GGTGGTAATTACCACAATGG+TGG | + | chr5_4:57812967-57812986 | Msa0859490:CDS | 45.0% | |
| ! | GGTGGTGGTAATTACCACAA+TGG | + | chr5_4:57812970-57812989 | Msa0859490:CDS | 45.0% |
| GGTAATTACCACCACCATTG+TGG | - | chr5_4:57812974-57812993 | Msa0859500:intron | 45.0% | |
| TATGGAGGTGGTTCTCACCA+CGG | + | chr5_4:57812769-57812788 | Msa0859490:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | + | chr5_4:57812781-57812800 | Msa0859490:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | + | chr5_4:57812784-57812803 | Msa0859490:CDS | 50.0% |
| !! | GGTGGTGGTGGACATTACTA+TGG | + | chr5_4:57812787-57812806 | Msa0859490:CDS | 50.0% |
| !! | GATGCCAAATATGGTGGTGG+TGG | + | chr5_4:57812799-57812818 | Msa0859490:CDS | 50.0% |
| AGCTTGCACGGCAACAAACT+CGG | - | chr5_4:57812843-57812862 | Msa0859500:CDS | 50.0% | |
| ACTCGGCATATGAGCAGCAA+TGG | - | chr5_4:57812860-57812879 | Msa0859500:CDS | 50.0% | |
| AATGGCCGTGGCAATAGTGA+TGG | - | chr5_4:57812878-57812897 | Msa0859500:CDS | 50.0% | |
| GGTTGCCATCACTATTGCCA+CGG | + | chr5_4:57812886-57812905 | Msa0859490:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | + | chr5_4:57812781-57812800 | Msa0859490:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | + | chr5_4:57812784-57812803 | Msa0859490:CDS | 50.0% |
| TATGGCGGTTACCACAATGG+TGG | + | chr5_4:57812988-57813007 | Msa0859490:CDS | 50.0% | |
| AATGGCAACCACCTCCACCA+TGG | - | chr5_4:57812728-57812747 | Msa0859500:CDS | 55.0% | |
| ! | GTTTGTTGCCGTGCAAGCTG+AGG | + | chr5_4:57812842-57812861 | Msa0859490:CDS | 55.0% |
| CATATGAGCAGCAATGGCCG+TGG | - | chr5_4:57812866-57812885 | Msa0859500:CDS | 55.0% | |
| TATGGAGGTGGTTCTCACGG+TGG | + | chr5_4:57812934-57812953 | Msa0859490:CDS | 55.0% | |
| ! | GGTTCTCACCATGGTGGAGG+TGG | + | chr5_4:57812739-57812758 | Msa0859490:CDS | 60.0% |
| GGTGGTTCTCACCATGGTGG+AGG | + | chr5_4:57812742-57812761 | Msa0859490:CDS | 60.0% | |
| GGAGGTGGTTCTCACCATGG+TGG | + | chr5_4:57812745-57812764 | Msa0859490:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+TGG | + | chr5_4:57812748-57812767 | Msa0859490:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+CGG | + | chr5_4:57812748-57812767 | Msa0859490:CDS | 60.0% | |
| GGCGGTTACCACAATGGTGG+TGG | + | chr5_4:57812985-57813004 | Msa0859490:CDS | 60.0% | |
| GGTGAGAACCACCTCCACCG+TGG | - | chr5_4:57812749-57812768 | Msa0859500:CDS | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | + | chr5_4:57812760-57812779 | Msa0859490:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | + | chr5_4:57812763-57812782 | Msa0859490:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | + | chr5_4:57812766-57812785 | Msa0859490:CDS | 65.0% | |
| GATGGCAACCACCTCCACCG+TGG | - | chr5_4:57812896-57812915 | Msa0859500:intron | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | + | chr5_4:57812760-57812779 | Msa0859490:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | + | chr5_4:57812763-57812782 | Msa0859490:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | + | chr5_4:57812766-57812785 | Msa0859490:CDS | 65.0% | |
| GGTGGTTCTCACGGTGGAGG+TGG | + | chr5_4:57812928-57812947 | Msa0859490:CDS | 65.0% | |
| GGAGGTGGTTCTCACGGTGG+AGG | + | chr5_4:57812931-57812950 | Msa0859490:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 57812711 | 57813046 | 57812711 | ID=Msa0859500;Name=Msa0859500 |
| chr5_4 | mRNA | 57812711 | 57813046 | 57812711 | ID=Msa0859500-mRNA-1;Parent=Msa0859500;Name=Msa0859500-mRNA-1;_AED=0.13;_eAED=0.18;_QI=0|0|0.5|1|0|0|2|16|81 |
| chr5_4 | exon | 57812711 | 57812912 | 57812711 | ID=Msa0859500-mRNA-1:exon:17650;Parent=Msa0859500-mRNA-1 |
| chr5_4 | exon | 57812987 | 57813046 | 57812987 | ID=Msa0859500-mRNA-1:exon:17649;Parent=Msa0859500-mRNA-1 |
| chr5_4 | CDS | 57812987 | 57813046 | 57812987 | ID=Msa0859500-mRNA-1:cds;Parent=Msa0859500-mRNA-1 |
| chr5_4 | CDS | 57812727 | 57812912 | 57812727 | ID=Msa0859500-mRNA-1:cds;Parent=Msa0859500-mRNA-1 |
| chr5_4 | three_prime_UTR | 57812711 | 57812726 | 57812711 | ID=Msa0859500-mRNA-1:three_prime_utr;Parent=Msa0859500-mRNA-1 |
| Gene Sequence |
| Protein sequence |
