Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0609970 | Msa0819390 | 0.805073 | 1.591706e-49 | -8.615850e-47 |
| Msa0622340 | Msa0819390 | 0.811169 | 8.007783e-51 | -8.615850e-47 |
| Msa0650620 | Msa0819390 | 0.816038 | 6.794717e-52 | -8.615850e-47 |
| Msa0692110 | Msa0819390 | 0.806692 | 7.271348e-50 | -8.615850e-47 |
| Msa0738850 | Msa0819390 | 0.814541 | 1.461537e-51 | -8.615850e-47 |
| Msa0738860 | Msa0819390 | 0.833371 | 5.542742e-56 | -8.615850e-47 |
| Msa0738870 | Msa0819390 | 0.810002 | 1.431077e-50 | -8.615850e-47 |
| Msa0738880 | Msa0819390 | 0.820905 | 5.356460e-53 | -8.615850e-47 |
| Msa0738890 | Msa0819390 | 0.928528 | 2.541569e-92 | -8.615850e-47 |
| Msa0738900 | Msa0819390 | 0.991052 | 1.203025e-185 | -8.615850e-47 |
| Msa0783270 | Msa0819390 | 0.803954 | 2.724272e-49 | -8.615850e-47 |
| Msa0783290 | Msa0819390 | 0.813761 | 2.173523e-51 | -8.615850e-47 |
| Msa0819320 | Msa0819390 | 0.807078 | 6.024471e-50 | -8.615850e-47 |
| Msa0819360 | Msa0819390 | 0.833572 | 4.938858e-56 | -8.615850e-47 |
| Msa0819370 | Msa0819390 | 0.818497 | 1.900978e-52 | -8.615850e-47 |
| Msa0819380 | Msa0819390 | 0.933562 | 1.552979e-95 | -8.615850e-47 |
| Msa0819390 | Msa0859490 | 0.935201 | 1.230286e-96 | -8.615850e-47 |
| Msa0819390 | Msa0859500 | 0.956685 | 1.645820e-114 | -8.615850e-47 |
| Msa0819390 | Msa0859520 | 0.824387 | 8.297573e-54 | -8.615850e-47 |
| Msa0819390 | Msa0859530 | 0.849426 | 3.259497e-60 | -8.615850e-47 |
| Msa0819390 | Msa0922540 | 0.828734 | 7.624928e-55 | -8.615850e-47 |
| Msa0819390 | Msa0960150 | -0.801054 | 1.078772e-48 | -8.615850e-47 |
| Msa0819390 | Msa0996090 | 0.815291 | 9.967990e-52 | -8.615850e-47 |
| Msa0819390 | Msa1002040 | 0.812141 | 4.922922e-51 | -8.615850e-47 |
| Msa0819390 | Msa1218650 | 0.817662 | 2.936170e-52 | -8.615850e-47 |
| Msa0819390 | Msa1224670 | 0.803168 | 3.964549e-49 | -8.615850e-47 |
| Msa0819390 | Msa1439230 | 0.808779 | 2.619377e-50 | -8.615850e-47 |
| Msa0168640 | Msa0819390 | -0.801564 | 8.483034e-49 | -8.615850e-47 |
| Msa0419640 | Msa0819390 | -0.816353 | 5.777791e-52 | -8.615850e-47 |
| Msa0426810 | Msa0819390 | 0.828138 | 1.061850e-54 | -8.615850e-47 |
| Msa0295580 | Msa0819390 | 0.827920 | 1.198323e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTGGTAACCGCCATATT+TGG | 0.317393 | 5_3:+55869329 | Msa0819390:three_prime_UTR |
| GAAGTAAACGATGCCAAATA+TGG | 0.333960 | 5_3:-55869342 | Msa0819380:CDS |
| AATGGTGGTGGACATTACTA+TGG | 0.429163 | 5_3:-55869288 | Msa0819380:CDS |
| GGTGGTTCTCACCATGGTGG+AGG | 0.471933 | 5_3:-55869078 | Msa0819380:CDS |
| GGTGGTTCTCACGGTGGAGG+TGG | 0.475576 | 5_3:-55869264 | Msa0819380:CDS |
| GGTTCTCACCATGGTGGAGG+TGG | 0.480643 | 5_3:-55869075 | Msa0819380:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_3:-55869099 | Msa0819380:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_3:-55869246 | Msa0819380:CDS |
| GGAGGTGGTTCTCACGGTGG+AGG | 0.512830 | 5_3:-55869267 | Msa0819380:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_3:-55869096 | Msa0819380:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_3:-55869243 | Msa0819380:CDS |
| TACTATGGAGGTGGTTCTCA+CGG | 0.525230 | 5_3:-55869273 | Msa0819380:CDS |
| ATTCAGCATGTGAGCAGCAA+TGG | 0.536032 | 5_3:+55869031 | Msa0819390:CDS |
| GGTAATTACCACAATGGTGG+TGG | 0.547595 | 5_3:-55869300 | Msa0819380:CDS |
| CATGTGAGCAGCAATGGCCG+TGG | 0.551382 | 5_3:+55869037 | Msa0819390:CDS |
| GGCGGTTACCACAATGGTGG+TGG | 0.554658 | 5_3:-55869321 | Msa0819380:CDS |
| GGTGGACATTACTATGGAGG+TGG | 0.556814 | 5_3:-55869282 | Msa0819380:CDS |
| CATATGAGCAGCAATGGCCG+TGG | 0.561023 | 5_3:+55869205 | Msa0819390:CDS |
| GGTTGCCATTACTATTGCCA+CGG | 0.599690 | 5_3:-55869054 | Msa0819380:CDS |
| AATGGCCGTGGCAATAGTAA+TGG | 0.601894 | 5_3:+55869049 | Msa0819390:CDS |
| AAATATGGCGGTTACCACAA+TGG | 0.601953 | 5_3:-55869327 | Msa0819380:CDS |
| ACTCGGCATATGAGCAGCAA+TGG | 0.602148 | 5_3:+55869199 | Msa0819390:CDS |
| AGCTTGCACGGCAACAAACT+CGG | 0.602417 | 5_3:+55869182 | Msa0819390:intron |
| GGTAATTACCACCACCATTG+TGG | 0.604720 | 5_3:+55869313 | Msa0819390:three_prime_UTR |
| GGTGGAGGTGGTTCTCACCA+TGG | 0.610718 | 5_3:-55869084 | Msa0819380:CDS |
| GGTTGCCATCACTATTGCCA+CGG | 0.613013 | 5_3:-55869222 | Msa0819380:CDS |
| AATGGCCGTGGCAATAGTGA+TGG | 0.615442 | 5_3:+55869217 | Msa0819390:exon |
| GGTGGAGGTGGTTCTCACCA+CGG | 0.641021 | 5_3:-55869252 | Msa0819380:CDS |
| AGTAATGTCCACCACCATTG+TGG | 0.641701 | 5_3:+55869292 | Msa0819390:three_prime_UTR |
| GGAGGTGGTTCTCACCATGG+TGG | 0.655820 | 5_3:-55869081 | Msa0819380:CDS |
| ATTTGTTGCCGTGCAAGCTG+AGG | 0.673816 | 5_3:-55869010 | Msa0819380:CDS |
| AATGGCAACCACCTCCACCA+TGG | 0.681347 | 5_3:+55869067 | Msa0819390:CDS |
| GGTGGTAATTACCACAATGG+TGG | 0.685453 | 5_3:-55869303 | Msa0819380:CDS |
| GGTGGTGGACATTACTATGG+AGG | 0.685934 | 5_3:-55869285 | Msa0819380:CDS |
| GTAAACGATGCCAAATATGG+CGG | 0.688367 | 5_3:-55869339 | Msa0819380:CDS |
| TATGGAGGTGGTTCTCACGG+TGG | 0.695015 | 5_3:-55869270 | Msa0819380:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.700953 | 5_3:-55869249 | Msa0819380:CDS |
| TATGGCGGTTACCACAATGG+TGG | 0.720585 | 5_3:-55869324 | Msa0819380:CDS |
| GGTGGTGGTAATTACCACAA+TGG | 0.736293 | 5_3:-55869306 | Msa0819380:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.747901 | 5_3:-55869102 | Msa0819380:CDS |
| GGTGAGAACCACCTCCACCG+TGG | 0.755651 | 5_3:+55869088 | Msa0819390:CDS |
| GATGGCAACCACCTCCACCG+TGG | 0.756569 | 5_3:+55869235 | Msa0819390:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGTAAACGATGCCAAATA+TGG | - | chr5_3:55869147-55869166 | Msa0819380:CDS | 35.0% | |
| GAAGTAAACGATGCCAAATA+TGG | - | chr5_3:55869147-55869166 | Msa0819380:CDS | 35.0% | |
| GTAAACGATGCCAAATATGG+TGG | - | chr5_3:55869144-55869163 | Msa0819380:CDS | 40.0% | |
| !! | AATGGTGGTGGACATTACTA+TGG | - | chr5_3:55869291-55869310 | Msa0819380:CDS | 40.0% |
| AAATATGGCGGTTACCACAA+TGG | - | chr5_3:55869330-55869349 | Msa0819380:CDS | 40.0% | |
| ATTGTGGTAACCGCCATATT+TGG | + | chr5_3:55869329-55869348 | Msa0819390:three_prime_UTR | 40.0% | |
| GTAAACGATGCCAAATATGG+CGG | - | chr5_3:55869144-55869163 | Msa0819380:CDS | 40.0% | |
| !! | AGTTTTGTCCTCAGCTTGCA+CGG | + | chr5_3:55869002-55869021 | Msa0819390:CDS | 45.0% |
| ATTCAGCATGTGAGCAGCAA+TGG | + | chr5_3:55869031-55869050 | Msa0819390:CDS | 45.0% | |
| AATGGCCGTGGCAATAGTAA+TGG | + | chr5_3:55869049-55869068 | Msa0819390:CDS | 45.0% | |
| GGTTGCCATTACTATTGCCA+CGG | - | chr5_3:55869057-55869076 | Msa0819380:CDS | 45.0% | |
| ATGTCCACCACCACCATATT+TGG | + | chr5_3:55869131-55869150 | Msa0819390:intron | 45.0% | |
| AACGATGCCAAATATGGTGG+TGG | - | chr5_3:55869141-55869160 | Msa0819380:CDS | 45.0% | |
| ! | AGTCTTTTCCTCAGCTTGCA+CGG | + | chr5_3:55869170-55869189 | Msa0819390:intron | 45.0% |
| TACTATGGAGGTGGTTCTCA+CGG | - | chr5_3:55869111-55869130 | Msa0819380:CDS | 45.0% | |
| AGTAATGTCCACCACCATTG+TGG | + | chr5_3:55869292-55869311 | Msa0819390:three_prime_UTR | 45.0% | |
| GGTAATTACCACAATGGTGG+TGG | - | chr5_3:55869303-55869322 | Msa0819380:CDS | 45.0% | |
| GGTGGTAATTACCACAATGG+TGG | - | chr5_3:55869306-55869325 | Msa0819380:CDS | 45.0% | |
| ! | GGTGGTGGTAATTACCACAA+TGG | - | chr5_3:55869309-55869328 | Msa0819380:CDS | 45.0% |
| GGTAATTACCACCACCATTG+TGG | + | chr5_3:55869313-55869332 | Msa0819390:three_prime_UTR | 45.0% | |
| ! | ATTTGTTGCCGTGCAAGCTG+AGG | - | chr5_3:55869013-55869032 | Msa0819380:CDS | 50.0% |
| TATGGAGGTGGTTCTCACCA+CGG | - | chr5_3:55869108-55869127 | Msa0819380:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | - | chr5_3:55869120-55869139 | Msa0819380:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | - | chr5_3:55869123-55869142 | Msa0819380:CDS | 50.0% |
| !! | GGTGGTGGTGGACATTACTA+TGG | - | chr5_3:55869126-55869145 | Msa0819380:CDS | 50.0% |
| !! | GATGCCAAATATGGTGGTGG+TGG | - | chr5_3:55869138-55869157 | Msa0819380:CDS | 50.0% |
| AGCTTGCACGGCAACAAACT+CGG | + | chr5_3:55869182-55869201 | Msa0819390:intron | 50.0% | |
| ACTCGGCATATGAGCAGCAA+TGG | + | chr5_3:55869199-55869218 | Msa0819390:CDS | 50.0% | |
| AATGGCCGTGGCAATAGTGA+TGG | + | chr5_3:55869217-55869236 | Msa0819390:exon | 50.0% | |
| GGTTGCCATCACTATTGCCA+CGG | - | chr5_3:55869225-55869244 | Msa0819380:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | - | chr5_3:55869120-55869139 | Msa0819380:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | - | chr5_3:55869123-55869142 | Msa0819380:CDS | 50.0% |
| TATGGCGGTTACCACAATGG+TGG | - | chr5_3:55869327-55869346 | Msa0819380:CDS | 50.0% | |
| AATGGCAACCACCTCCACCA+TGG | + | chr5_3:55869067-55869086 | Msa0819390:CDS | 55.0% | |
| ! | GTTTGTTGCCGTGCAAGCTG+AGG | - | chr5_3:55869181-55869200 | Msa0819380:CDS | 55.0% |
| CATATGAGCAGCAATGGCCG+TGG | + | chr5_3:55869205-55869224 | Msa0819390:CDS | 55.0% | |
| TATGGAGGTGGTTCTCACGG+TGG | - | chr5_3:55869273-55869292 | Msa0819380:CDS | 55.0% | |
| CATGTGAGCAGCAATGGCCG+TGG | + | chr5_3:55869037-55869056 | Msa0819390:CDS | 60.0% | |
| ! | GGTTCTCACCATGGTGGAGG+TGG | - | chr5_3:55869078-55869097 | Msa0819380:CDS | 60.0% |
| GGTGGTTCTCACCATGGTGG+AGG | - | chr5_3:55869081-55869100 | Msa0819380:CDS | 60.0% | |
| GGAGGTGGTTCTCACCATGG+TGG | - | chr5_3:55869084-55869103 | Msa0819380:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+TGG | - | chr5_3:55869087-55869106 | Msa0819380:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+CGG | - | chr5_3:55869087-55869106 | Msa0819380:CDS | 60.0% | |
| GGCGGTTACCACAATGGTGG+TGG | - | chr5_3:55869324-55869343 | Msa0819380:CDS | 60.0% | |
| GGTGAGAACCACCTCCACCG+TGG | + | chr5_3:55869088-55869107 | Msa0819390:CDS | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | - | chr5_3:55869099-55869118 | Msa0819380:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | - | chr5_3:55869102-55869121 | Msa0819380:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | - | chr5_3:55869105-55869124 | Msa0819380:CDS | 65.0% | |
| GATGGCAACCACCTCCACCG+TGG | + | chr5_3:55869235-55869254 | Msa0819390:three_prime_UTR | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | - | chr5_3:55869099-55869118 | Msa0819380:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | - | chr5_3:55869102-55869121 | Msa0819380:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | - | chr5_3:55869105-55869124 | Msa0819380:CDS | 65.0% | |
| GGTGGTTCTCACGGTGGAGG+TGG | - | chr5_3:55869267-55869286 | Msa0819380:CDS | 65.0% | |
| GGAGGTGGTTCTCACGGTGG+AGG | - | chr5_3:55869270-55869289 | Msa0819380:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 55868941 | 55869385 | 55868941 | ID=Msa0819390;Name=Msa0819390 |
| chr5_3 | mRNA | 55868941 | 55869385 | 55868941 | ID=Msa0819390-mRNA-1;Parent=Msa0819390;Name=Msa0819390-mRNA-1;_AED=0.25;_eAED=0.29;_QI=0|0|0.5|1|0|0|2|152|72 |
| chr5_3 | exon | 55868941 | 55869109 | 55868941 | ID=Msa0819390-mRNA-1:exon:16860;Parent=Msa0819390-mRNA-1 |
| chr5_3 | exon | 55869184 | 55869385 | 55869184 | ID=Msa0819390-mRNA-1:exon:16861;Parent=Msa0819390-mRNA-1 |
| chr5_3 | CDS | 55868941 | 55869109 | 55868941 | ID=Msa0819390-mRNA-1:cds;Parent=Msa0819390-mRNA-1 |
| chr5_3 | CDS | 55869184 | 55869233 | 55869184 | ID=Msa0819390-mRNA-1:cds;Parent=Msa0819390-mRNA-1 |
| chr5_3 | three_prime_UTR | 55869234 | 55869385 | 55869234 | ID=Msa0819390-mRNA-1:three_prime_utr;Parent=Msa0819390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
