Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0609970 | Msa0738900 | 0.807081 | 6.015682e-50 | -8.615850e-47 |
| Msa0622340 | Msa0738900 | 0.803788 | 2.949526e-49 | -8.615850e-47 |
| Msa0650620 | Msa0738900 | 0.817202 | 3.727166e-52 | -8.615850e-47 |
| Msa0692110 | Msa0738900 | 0.800522 | 1.385336e-48 | -8.615850e-47 |
| Msa0738850 | Msa0738900 | 0.806126 | 9.569513e-50 | -8.615850e-47 |
| Msa0738860 | Msa0738900 | 0.822078 | 2.870558e-53 | -8.615850e-47 |
| Msa0738880 | Msa0738900 | 0.819286 | 1.257648e-52 | -8.615850e-47 |
| Msa0738890 | Msa0738900 | 0.934249 | 5.412931e-96 | -8.615850e-47 |
| Msa0738900 | Msa0783290 | 0.809904 | 1.502437e-50 | -8.615850e-47 |
| Msa0738900 | Msa0819320 | 0.805296 | 1.429426e-49 | -8.615850e-47 |
| Msa0738900 | Msa0819360 | 0.826569 | 2.524623e-54 | -8.615850e-47 |
| Msa0738900 | Msa0819370 | 0.816869 | 4.426102e-52 | -8.615850e-47 |
| Msa0738900 | Msa0819380 | 0.933794 | 1.088297e-95 | -8.615850e-47 |
| Msa0738900 | Msa0819390 | 0.991052 | 1.203025e-185 | -8.615850e-47 |
| Msa0738900 | Msa0859490 | 0.940414 | 2.430480e-100 | -8.615850e-47 |
| Msa0738900 | Msa0859500 | 0.957049 | 6.903569e-115 | -8.615850e-47 |
| Msa0738900 | Msa0859510 | 0.805033 | 1.622895e-49 | -8.615850e-47 |
| Msa0738900 | Msa0859520 | 0.816856 | 4.455928e-52 | -8.615850e-47 |
| Msa0738900 | Msa0859530 | 0.842117 | 3.145508e-58 | -8.615850e-47 |
| Msa0738900 | Msa0922540 | 0.814365 | 1.599054e-51 | -8.615850e-47 |
| Msa0738900 | Msa0960150 | -0.806226 | 9.115986e-50 | -8.615850e-47 |
| Msa0738900 | Msa0996090 | 0.803009 | 4.277324e-49 | -8.615850e-47 |
| Msa0738900 | Msa1218650 | 0.810595 | 1.066243e-50 | -8.615850e-47 |
| Msa0419640 | Msa0738900 | -0.811930 | 5.471267e-51 | -8.615850e-47 |
| Msa0426810 | Msa0738900 | 0.821321 | 4.296644e-53 | -8.615850e-47 |
| Msa0295580 | Msa0738900 | 0.824830 | 6.523981e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTGGTAACCGCCATATT+TGG | 0.317393 | 5_1:+56971946 | Msa0738900:three_prime_UTR |
| GAAGTAAACGATGCCAAATA+TGG | 0.333960 | 5_1:-56971959 | Msa0738890:CDS |
| AATGGTGGTGGACATTACTA+TGG | 0.429163 | 5_1:-56971905 | Msa0738890:CDS |
| GGTGGTTCTCACCATGGTGG+AGG | 0.471933 | 5_1:-56971695 | Msa0738890:CDS |
| GGTGGTTCTCACGGTGGAGG+TGG | 0.475576 | 5_1:-56971881 | Msa0738890:CDS |
| GGTTCTCACCATGGTGGAGG+TGG | 0.480643 | 5_1:-56971692 | Msa0738890:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_1:-56971716 | Msa0738890:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5_1:-56971863 | Msa0738890:CDS |
| GGAGGTGGTTCTCACGGTGG+AGG | 0.512830 | 5_1:-56971884 | Msa0738890:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_1:-56971713 | Msa0738890:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5_1:-56971860 | Msa0738890:CDS |
| TACTATGGAGGTGGTTCTCA+CGG | 0.525230 | 5_1:-56971890 | Msa0738890:CDS |
| ATTCAGCATGTGAGCAGCAA+TGG | 0.536032 | 5_1:+56971648 | Msa0738900:CDS |
| GGTAATTACCACAATGGTGG+TGG | 0.547595 | 5_1:-56971917 | Msa0738890:CDS |
| CATGTGAGCAGCAATGGCCG+TGG | 0.551382 | 5_1:+56971654 | Msa0738900:CDS |
| GGCGGTTACCACAATGGTGG+TGG | 0.554658 | 5_1:-56971938 | Msa0738890:CDS |
| GGTGGACATTACTATGGAGG+TGG | 0.556814 | 5_1:-56971899 | Msa0738890:CDS |
| CATATGAGCAGCAATGGCCG+TGG | 0.561023 | 5_1:+56971822 | Msa0738900:CDS |
| GGTTGCCATTACTATTGCCA+CGG | 0.599690 | 5_1:-56971671 | Msa0738890:CDS |
| AATGGCCGTGGCAATAGTAA+TGG | 0.601894 | 5_1:+56971666 | Msa0738900:CDS |
| AAATATGGCGGTTACCACAA+TGG | 0.601953 | 5_1:-56971944 | Msa0738890:CDS |
| ACTCGGCATATGAGCAGCAA+TGG | 0.602148 | 5_1:+56971816 | Msa0738900:CDS |
| AGCTTGCACGGCAACAAACT+CGG | 0.602417 | 5_1:+56971799 | Msa0738900:intron |
| GGTAATTACCACCACCATTG+TGG | 0.604720 | 5_1:+56971930 | Msa0738900:three_prime_UTR |
| GGTGGAGGTGGTTCTCACCA+TGG | 0.610718 | 5_1:-56971701 | Msa0738890:CDS |
| GGTTGCCATCACTATTGCCA+CGG | 0.613013 | 5_1:-56971839 | Msa0738890:CDS |
| AATGGCCGTGGCAATAGTGA+TGG | 0.615442 | 5_1:+56971834 | Msa0738900:exon |
| GGTGGAGGTGGTTCTCACCA+CGG | 0.641021 | 5_1:-56971869 | Msa0738890:CDS |
| AGTAATGTCCACCACCATTG+TGG | 0.641701 | 5_1:+56971909 | Msa0738900:three_prime_UTR |
| GGAGGTGGTTCTCACCATGG+TGG | 0.655820 | 5_1:-56971698 | Msa0738890:CDS |
| ATTTGTTGCCGTGCAAGCTG+AGG | 0.673816 | 5_1:-56971627 | Msa0738890:CDS |
| AATGGCAACCACCTCCACCA+TGG | 0.681347 | 5_1:+56971684 | Msa0738900:CDS |
| GGTGGTAATTACCACAATGG+TGG | 0.685453 | 5_1:-56971920 | Msa0738890:CDS |
| GGTGGTGGACATTACTATGG+AGG | 0.685934 | 5_1:-56971902 | Msa0738890:CDS |
| GTAAACGATGCCAAATATGG+CGG | 0.688367 | 5_1:-56971956 | Msa0738890:CDS |
| TATGGAGGTGGTTCTCACGG+TGG | 0.695015 | 5_1:-56971887 | Msa0738890:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.700953 | 5_1:-56971866 | Msa0738890:CDS |
| TATGGCGGTTACCACAATGG+TGG | 0.720585 | 5_1:-56971941 | Msa0738890:CDS |
| GGTGGTGGTAATTACCACAA+TGG | 0.736293 | 5_1:-56971923 | Msa0738890:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.747901 | 5_1:-56971719 | Msa0738890:CDS |
| GGTGAGAACCACCTCCACCG+TGG | 0.755651 | 5_1:+56971705 | Msa0738900:CDS |
| GATGGCAACCACCTCCACCG+TGG | 0.756569 | 5_1:+56971852 | Msa0738900:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGTAAACGATGCCAAATA+TGG | - | chr5_1:56971764-56971783 | Msa0738890:CDS | 35.0% | |
| GAAGTAAACGATGCCAAATA+TGG | - | chr5_1:56971764-56971783 | Msa0738890:CDS | 35.0% | |
| GTAAACGATGCCAAATATGG+TGG | - | chr5_1:56971761-56971780 | Msa0738890:CDS | 40.0% | |
| !! | AATGGTGGTGGACATTACTA+TGG | - | chr5_1:56971908-56971927 | Msa0738890:CDS | 40.0% |
| AAATATGGCGGTTACCACAA+TGG | - | chr5_1:56971947-56971966 | Msa0738890:CDS | 40.0% | |
| ATTGTGGTAACCGCCATATT+TGG | + | chr5_1:56971946-56971965 | Msa0738900:three_prime_UTR | 40.0% | |
| GTAAACGATGCCAAATATGG+CGG | - | chr5_1:56971761-56971780 | Msa0738890:CDS | 40.0% | |
| !! | AGTTTTGTCCTCAGCTTGCA+CGG | + | chr5_1:56971619-56971638 | Msa0738900:CDS | 45.0% |
| ATTCAGCATGTGAGCAGCAA+TGG | + | chr5_1:56971648-56971667 | Msa0738900:CDS | 45.0% | |
| AATGGCCGTGGCAATAGTAA+TGG | + | chr5_1:56971666-56971685 | Msa0738900:CDS | 45.0% | |
| GGTTGCCATTACTATTGCCA+CGG | - | chr5_1:56971674-56971693 | Msa0738890:CDS | 45.0% | |
| ATGTCCACCACCACCATATT+TGG | + | chr5_1:56971748-56971767 | Msa0738900:intron | 45.0% | |
| AACGATGCCAAATATGGTGG+TGG | - | chr5_1:56971758-56971777 | Msa0738890:CDS | 45.0% | |
| ! | AGTCTTTTCCTCAGCTTGCA+CGG | + | chr5_1:56971787-56971806 | Msa0738900:intron | 45.0% |
| TACTATGGAGGTGGTTCTCA+CGG | - | chr5_1:56971728-56971747 | Msa0738890:CDS | 45.0% | |
| AGTAATGTCCACCACCATTG+TGG | + | chr5_1:56971909-56971928 | Msa0738900:three_prime_UTR | 45.0% | |
| GGTAATTACCACAATGGTGG+TGG | - | chr5_1:56971920-56971939 | Msa0738890:CDS | 45.0% | |
| GGTGGTAATTACCACAATGG+TGG | - | chr5_1:56971923-56971942 | Msa0738890:CDS | 45.0% | |
| ! | GGTGGTGGTAATTACCACAA+TGG | - | chr5_1:56971926-56971945 | Msa0738890:CDS | 45.0% |
| GGTAATTACCACCACCATTG+TGG | + | chr5_1:56971930-56971949 | Msa0738900:three_prime_UTR | 45.0% | |
| ! | ATTTGTTGCCGTGCAAGCTG+AGG | - | chr5_1:56971630-56971649 | Msa0738890:CDS | 50.0% |
| TATGGAGGTGGTTCTCACCA+CGG | - | chr5_1:56971725-56971744 | Msa0738890:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | - | chr5_1:56971737-56971756 | Msa0738890:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | - | chr5_1:56971740-56971759 | Msa0738890:CDS | 50.0% |
| !! | GGTGGTGGTGGACATTACTA+TGG | - | chr5_1:56971743-56971762 | Msa0738890:CDS | 50.0% |
| !! | GATGCCAAATATGGTGGTGG+TGG | - | chr5_1:56971755-56971774 | Msa0738890:CDS | 50.0% |
| AGCTTGCACGGCAACAAACT+CGG | + | chr5_1:56971799-56971818 | Msa0738900:intron | 50.0% | |
| ACTCGGCATATGAGCAGCAA+TGG | + | chr5_1:56971816-56971835 | Msa0738900:CDS | 50.0% | |
| AATGGCCGTGGCAATAGTGA+TGG | + | chr5_1:56971834-56971853 | Msa0738900:exon | 50.0% | |
| GGTTGCCATCACTATTGCCA+CGG | - | chr5_1:56971842-56971861 | Msa0738890:CDS | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | - | chr5_1:56971737-56971756 | Msa0738890:CDS | 50.0% | |
| !! | GGTGGTGGACATTACTATGG+AGG | - | chr5_1:56971740-56971759 | Msa0738890:CDS | 50.0% |
| TATGGCGGTTACCACAATGG+TGG | - | chr5_1:56971944-56971963 | Msa0738890:CDS | 50.0% | |
| AATGGCAACCACCTCCACCA+TGG | + | chr5_1:56971684-56971703 | Msa0738900:CDS | 55.0% | |
| ! | GTTTGTTGCCGTGCAAGCTG+AGG | - | chr5_1:56971798-56971817 | Msa0738890:CDS | 55.0% |
| CATATGAGCAGCAATGGCCG+TGG | + | chr5_1:56971822-56971841 | Msa0738900:CDS | 55.0% | |
| TATGGAGGTGGTTCTCACGG+TGG | - | chr5_1:56971890-56971909 | Msa0738890:CDS | 55.0% | |
| CATGTGAGCAGCAATGGCCG+TGG | + | chr5_1:56971654-56971673 | Msa0738900:CDS | 60.0% | |
| ! | GGTTCTCACCATGGTGGAGG+TGG | - | chr5_1:56971695-56971714 | Msa0738890:CDS | 60.0% |
| GGTGGTTCTCACCATGGTGG+AGG | - | chr5_1:56971698-56971717 | Msa0738890:CDS | 60.0% | |
| GGAGGTGGTTCTCACCATGG+TGG | - | chr5_1:56971701-56971720 | Msa0738890:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+TGG | - | chr5_1:56971704-56971723 | Msa0738890:CDS | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+CGG | - | chr5_1:56971704-56971723 | Msa0738890:CDS | 60.0% | |
| GGCGGTTACCACAATGGTGG+TGG | - | chr5_1:56971941-56971960 | Msa0738890:CDS | 60.0% | |
| GGTGAGAACCACCTCCACCG+TGG | + | chr5_1:56971705-56971724 | Msa0738900:CDS | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | - | chr5_1:56971716-56971735 | Msa0738890:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | - | chr5_1:56971719-56971738 | Msa0738890:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | - | chr5_1:56971722-56971741 | Msa0738890:CDS | 65.0% | |
| GATGGCAACCACCTCCACCG+TGG | + | chr5_1:56971852-56971871 | Msa0738900:three_prime_UTR | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | - | chr5_1:56971716-56971735 | Msa0738890:CDS | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | - | chr5_1:56971719-56971738 | Msa0738890:CDS | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | - | chr5_1:56971722-56971741 | Msa0738890:CDS | 65.0% | |
| GGTGGTTCTCACGGTGGAGG+TGG | - | chr5_1:56971884-56971903 | Msa0738890:CDS | 65.0% | |
| GGAGGTGGTTCTCACGGTGG+AGG | - | chr5_1:56971887-56971906 | Msa0738890:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 56971558 | 56972002 | 56971558 | ID=Msa0738900;Name=Msa0738900 |
| chr5_1 | mRNA | 56971558 | 56972002 | 56971558 | ID=Msa0738900-mRNA-1;Parent=Msa0738900;Name=Msa0738900-mRNA-1;_AED=0.25;_eAED=0.29;_QI=0|0|0.5|1|0|0|2|152|72 |
| chr5_1 | exon | 56971558 | 56971726 | 56971558 | ID=Msa0738900-mRNA-1:exon:16102;Parent=Msa0738900-mRNA-1 |
| chr5_1 | exon | 56971801 | 56972002 | 56971801 | ID=Msa0738900-mRNA-1:exon:16103;Parent=Msa0738900-mRNA-1 |
| chr5_1 | CDS | 56971558 | 56971726 | 56971558 | ID=Msa0738900-mRNA-1:cds;Parent=Msa0738900-mRNA-1 |
| chr5_1 | CDS | 56971801 | 56971850 | 56971801 | ID=Msa0738900-mRNA-1:cds;Parent=Msa0738900-mRNA-1 |
| chr5_1 | three_prime_UTR | 56971851 | 56972002 | 56971851 | ID=Msa0738900-mRNA-1:three_prime_utr;Parent=Msa0738900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
