AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0864480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864480 MtrunA17_Chr5g0446831 100.000 204 0 0 1 204 1 204 5.51e-150 414
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864480 AT5G55940.1 72.330 206 55 2 1 204 3 208 1.93e-107 307
Msa0864480 AT5G51620.4 45.714 105 42 2 70 171 1 93 3.39e-22 89.0
Msa0864480 AT5G51620.3 76.000 50 12 0 70 119 1 50 3.65e-22 85.5
Msa0864480 AT5G51620.1 46.535 101 39 2 70 167 1 89 3.96e-21 85.1
Msa0864480 AT5G51620.2 49.206 63 32 0 70 132 1 63 2.05e-12 60.8

Find 71 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATGTAGGTGTATTAATT+TGG 0.198614 5_4:+64867188 Msa0864480:three_prime_UTR
AAACTCTGAAAATTGTTAAT+TGG 0.213034 5_4:-64867104 None:intergenic
ATCAGCAAATGTGATCTTAT+TGG 0.214457 5_4:+64866696 Msa0864480:CDS
ATTGGAGATCTCTCTTATTC+TGG 0.256125 5_4:+64864897 Msa0864480:CDS
TTATGGTTTAGCTTTATGTT+AGG 0.282779 5_4:+64866608 Msa0864480:intron
TCCACAAGCTGCTATTCTAT+TGG 0.321743 5_4:+64865533 Msa0864480:CDS
ATCCATTATCCTGTTTCATA+TGG 0.347732 5_4:+64867268 Msa0864480:three_prime_UTR
AATCCCTCTTCTTCCTCAAT+TGG 0.350775 5_4:+64864879 Msa0864480:CDS
GATTGTTCGTTACAAGAATT+CGG 0.353737 5_4:-64867006 None:intergenic
AACCATATGAAACAGGATAA+TGG 0.358913 5_4:-64867270 None:intergenic
ATTATATTTCATCTGAGAAA+TGG 0.367127 5_4:+64866719 Msa0864480:CDS
TATGGTTTAGCTTTATGTTA+GGG 0.373331 5_4:+64866609 Msa0864480:intron
ACCAACTATGTTCAACCCTT+TGG 0.413858 5_4:-64865427 None:intergenic
GATTTCGACGGTGTCGTTAG+AGG 0.416969 5_4:-64864827 None:intergenic
CAAAGTAGGTTGGATACAAA+TGG 0.421125 5_4:-64866946 None:intergenic
ACTTCCCATGCTTCTGATGA+TGG 0.424911 5_4:-64864695 None:intergenic
ACCAAAGGGTTGAACATAGT+TGG 0.427209 5_4:+64865426 Msa0864480:CDS
CTTAGCAAACCATATGAAAC+AGG 0.429072 5_4:-64867277 None:intergenic
AATTCTTGTAACGAACAATC+AGG 0.434217 5_4:+64867009 Msa0864480:three_prime_UTR
TTAGGGATGCATCTAAGAAC+TGG 0.438779 5_4:+64866626 Msa0864480:CDS
GGGATTTGAGAGTGGAAAAG+AGG 0.453984 5_4:-64864862 None:intergenic
GGATTTGAGAGTGGAAAAGA+GGG 0.454226 5_4:-64864861 None:intergenic
CGAGATCAGTTGAAGAGCCC+TGG 0.457489 5_4:-64866914 None:intergenic
ACGCCGTTGACGGCGGATGT+AGG 0.461476 5_4:-64864784 None:intergenic
CGCCGTTGACGGCGGATGTA+GGG 0.461819 5_4:-64864783 None:intergenic
TTTGTGGACTTCGATATGTT+TGG 0.462344 5_4:-64864598 None:intergenic
GCCGATCAAGACGCCGTTGA+CGG 0.463051 5_4:-64864794 None:intergenic
CACCTACATAAAAGTTAGAA+AGG 0.475710 5_4:-64867177 None:intergenic
GCCGTCAACGGCGTCTTGAT+CGG 0.478430 5_4:+64864793 Msa0864480:CDS
ACATCACCCACTTGCAAAGT+AGG 0.479916 5_4:-64866960 None:intergenic
CTGTTTCATATGGTTTGCTA+AGG 0.482939 5_4:+64867278 Msa0864480:three_prime_UTR
AAAATCATCAGACCTCTCAT+TGG 0.502605 5_4:-64865460 None:intergenic
ATCTCCAATTGAGGAAGAAG+AGG 0.512179 5_4:-64864883 None:intergenic
ATGGCTCTTTGAGAGAGAAT+CGG 0.512595 5_4:-64866675 None:intergenic
TCAGGGATTGGCTAAACCCA+GGG 0.530286 5_4:+64866897 Msa0864480:intron
TGCATCTAAGAACTGGAAGT+TGG 0.530544 5_4:+64866633 Msa0864480:CDS
CACCCACTTGCAAAGTAGGT+TGG 0.531937 5_4:-64866956 None:intergenic
TTCAGGGATTGGCTAAACCC+AGG 0.536967 5_4:+64866896 Msa0864480:intron
ATCATCCATCATCAGAAGCA+TGG 0.541246 5_4:+64864690 Msa0864480:exon
CCTACTTGCTTATATCATCA+AGG 0.542389 5_4:-64866768 None:intergenic
CACCCTACATCCGCCGTCAA+CGG 0.543335 5_4:+64864781 Msa0864480:CDS
GAAGAAGAGGGATTTGAGAG+TGG 0.543708 5_4:-64864870 None:intergenic
ACTTTATCAGATGTATAAGC+AGG 0.549532 5_4:+64867070 Msa0864480:three_prime_UTR
CAGGGCTCTTCAACTGATCT+CGG 0.551747 5_4:+64866915 Msa0864480:exon
GTGGAAAAGAGGGACGGCAT+CGG 0.554411 5_4:-64864851 None:intergenic
AAAGAAAATAGCGCAAAGGA+AGG 0.559428 5_4:-64867351 None:intergenic
TCATCCATCATCAGAAGCAT+GGG 0.563935 5_4:+64864691 Msa0864480:exon
GAGATCAGTTGAAGAGCCCT+GGG 0.564845 5_4:-64866913 None:intergenic
AAAATATGGCCCTTTGTGGA+AGG 0.566345 5_4:-64867128 None:intergenic
GTGGAAAGTAACGACAGATA+TGG 0.568666 5_4:-64865515 None:intergenic
GAGTAGCAGTGCTATAATGC+AGG 0.572270 5_4:+64865678 Msa0864480:CDS
GGTGATGTTCAAGCATATCA+TGG 0.573813 5_4:+64866975 Msa0864480:three_prime_UTR
AATAAGATCACATTTGCTGA+TGG 0.584824 5_4:-64866694 None:intergenic
ACCAATAGAATAGCAGCTTG+TGG 0.585214 5_4:-64865534 None:intergenic
AAATAGCGCAAAGGAAGGAA+AGG 0.588028 5_4:-64867346 None:intergenic
TATCCAACCTACTTTGCAAG+TGG 0.588446 5_4:+64866953 Msa0864480:three_prime_UTR
ATTCTTGTAACGAACAATCA+GGG 0.599699 5_4:+64867010 Msa0864480:three_prime_UTR
ATAAGAGAGATCTCCAATTG+AGG 0.613544 5_4:-64864892 None:intergenic
TTCGACGGTGTCGTTAGAGG+AGG 0.616044 5_4:-64864824 None:intergenic
GACGGCATCGGTGATTTCGA+CGG 0.623195 5_4:-64864839 None:intergenic
TCTCCAATTGAGGAAGAAGA+GGG 0.626207 5_4:-64864882 None:intergenic
ATCCAACCTACTTTGCAAGT+GGG 0.630371 5_4:+64866954 Msa0864480:three_prime_UTR
GATCAAGACGCCGTTGACGG+CGG 0.635153 5_4:-64864791 None:intergenic
AGGGAATAGATCTTGACTTG+AGG 0.636762 5_4:+64867029 Msa0864480:three_prime_UTR
GTTGGATACAAATGGACTGA+GGG 0.643356 5_4:-64866938 None:intergenic
GGTGGTGTGGCAAAGAACAT+TGG 0.647028 5_4:+64865489 Msa0864480:CDS
TTGAGAGTGGAAAAGAGGGA+CGG 0.649379 5_4:-64864857 None:intergenic
AAGTAACGATTTGAAGTACG+AGG 0.660885 5_4:+64864714 Msa0864480:CDS
GGTTGGATACAAATGGACTG+AGG 0.671901 5_4:-64866939 None:intergenic
TGTCGTTAGAGGAGGAGATG+CGG 0.688753 5_4:-64864816 None:intergenic
CAACACAGACAGTCACACGA+AGG 0.700677 5_4:-64864658 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTGTTTTATGAATTTTATTT+GGG + chr5_4:64865748-64865767 Msa0864480:intron 15.0%
!! TTTAATAACAAAAAAAGGAA+AGG - chr5_4:64866460-64866479 None:intergenic 15.0%
!!! TTTTAGACTTATATTTTCTA+AGG + chr5_4:64867313-64867332 Msa0864480:three_prime_UTR 15.0%
!!! ATTTAGCAGTTAACAATTTT+GGG - chr5_4:64864983-64865002 None:intergenic 20.0%
!!! AATTTAGCAGTTAACAATTT+TGG - chr5_4:64864984-64865003 None:intergenic 20.0%
!! AAAATTGTAATCATAGTTGT+TGG - chr5_4:64865255-64865274 None:intergenic 20.0%
!!! GCTGTTTTATGAATTTTATT+TGG + chr5_4:64865747-64865766 Msa0864480:intron 20.0%
!! TCTAGATAATGTTTAATCAA+GGG + chr5_4:64865776-64865795 Msa0864480:intron 20.0%
!! TTCACAGTAAATATAGAATT+CGG + chr5_4:64865915-64865934 Msa0864480:intron 20.0%
!!! AAAGAACTCCTTTTTTATAA+TGG + chr5_4:64866024-64866043 Msa0864480:intron 20.0%
!! CAATGATTCCATTATAAAAA+AGG - chr5_4:64866035-64866054 None:intergenic 20.0%
!! TTTGTAATTAGTACAAACAT+TGG - chr5_4:64866371-64866390 None:intergenic 20.0%
!! ATTATATTTCATCTGAGAAA+TGG + chr5_4:64866719-64866738 Msa0864480:CDS 20.0%
!! AATCAAGAAGAAAAAAGATT+GGG - chr5_4:64866822-64866841 None:intergenic 20.0%
!! AAATCAAGAAGAAAAAAGAT+TGG - chr5_4:64866823-64866842 None:intergenic 20.0%
!!! TATTATCCAATTTTGTTTTC+AGG + chr5_4:64866879-64866898 Msa0864480:intron 20.0%
!!! ATTATCCAATTTTGTTTTCA+GGG + chr5_4:64866880-64866899 Msa0864480:intron 20.0%
!!! AAACTCTGAAAATTGTTAAT+TGG - chr5_4:64867107-64867126 None:intergenic 20.0%
!! TTTATGTAGGTGTATTAATT+TGG + chr5_4:64867188-64867207 Msa0864480:three_prime_UTR 20.0%
! TAACATGCAATTTAATCATC+TGG + chr5_4:64865031-64865050 Msa0864480:intron 25.0%
! AACATGCAATTTAATCATCT+GGG + chr5_4:64865032-64865051 Msa0864480:intron 25.0%
!!! TGTACATTGACAATTTTGAT+CGG + chr5_4:64865122-64865141 Msa0864480:intron 25.0%
! AATTAGTGAATGTTCATGTT+TGG + chr5_4:64865187-64865206 Msa0864480:intron 25.0%
! CTCTAGATAATGTTTAATCA+AGG + chr5_4:64865775-64865794 Msa0864480:intron 25.0%
! TCACAGTAAATATAGAATTC+GGG + chr5_4:64865916-64865935 Msa0864480:intron 25.0%
! CAAATATCTAGAAATCCTTT+AGG + chr5_4:64865983-64866002 Msa0864480:intron 25.0%
! ATACGCATATTATAGTTCAA+AGG - chr5_4:64866061-64866080 None:intergenic 25.0%
!!! TTTTGACTTATGCTGAAAAT+AGG - chr5_4:64866198-64866217 None:intergenic 25.0%
!!! GATCTAAAATTTTGATGAGA+TGG + chr5_4:64866263-64866282 Msa0864480:intron 25.0%
! CTTACTCTGCTTAAATATAT+AGG + chr5_4:64866315-64866334 Msa0864480:intron 25.0%
! TAACAAAAAAAGGAAAGGAA+AGG - chr5_4:64866455-64866474 None:intergenic 25.0%
!!! AAAGATTGCCTTTTTTCTTA+TGG + chr5_4:64866591-64866610 Msa0864480:intron 25.0%
! AAGCTAAACCATAAGAAAAA+AGG - chr5_4:64866602-64866621 None:intergenic 25.0%
!!! TTATGGTTTAGCTTTATGTT+AGG + chr5_4:64866608-64866627 Msa0864480:intron 25.0%
!!! TATGGTTTAGCTTTATGTTA+GGG + chr5_4:64866609-64866628 Msa0864480:intron 25.0%
!! TGTAAAGTTGATTATAACTG+CGG - chr5_4:64866852-64866871 None:intergenic 25.0%
!! TTCCTTTCTAACTTTTATGT+AGG + chr5_4:64867175-64867194 Msa0864480:three_prime_UTR 25.0%
!! TGTTTGTGTTTGTGTTTTTG+TGG - chr5_4:64864617-64864636 None:intergenic 30.0%
!! GGTTGATCTTTATTTCTGTT+GGG + chr5_4:64865053-64865072 Msa0864480:intron 30.0%
ACAACAAAATCAGAAAACTG+TGG - chr5_4:64865358-64865377 None:intergenic 30.0%
! AGGAATACTTTTCTACCAAA+GGG + chr5_4:64865412-64865431 Msa0864480:CDS 30.0%
CACAGTAAATATAGAATTCG+GGG + chr5_4:64865917-64865936 Msa0864480:intron 30.0%
! TTTTGCATTTGACAATGTCT+GGG + chr5_4:64866094-64866113 Msa0864480:intron 30.0%
ATCTTGAAATTCAGATCTGA+TGG + chr5_4:64866131-64866150 Msa0864480:intron 30.0%
TTTCAGCATAAGTCAAAAAG+AGG + chr5_4:64866200-64866219 Msa0864480:intron 30.0%
TTCAGCATAAGTCAAAAAGA+GGG + chr5_4:64866201-64866220 Msa0864480:intron 30.0%
TGTAATCTCAATCCACTATA+AGG + chr5_4:64866339-64866358 Msa0864480:intron 30.0%
AAAGGAAAGGAAAGGAAAAT+AGG - chr5_4:64866447-64866466 None:intergenic 30.0%
AAAGATGGATCTACATCATA+TGG - chr5_4:64866554-64866573 None:intergenic 30.0%
! ATGTAGATCCATCTTTTTGT+TGG + chr5_4:64866558-64866577 Msa0864480:intron 30.0%
TAATGCAACCAACAAAAAGA+TGG - chr5_4:64866569-64866588 None:intergenic 30.0%
! AATAAGATCACATTTGCTGA+TGG - chr5_4:64866697-64866716 None:intergenic 30.0%
ATCAGCAAATGTGATCTTAT+TGG + chr5_4:64866696-64866715 Msa0864480:CDS 30.0%
GATTGTTCGTTACAAGAATT+CGG - chr5_4:64867009-64867028 None:intergenic 30.0%
AATTCTTGTAACGAACAATC+AGG + chr5_4:64867009-64867028 Msa0864480:three_prime_UTR 30.0%
ATTCTTGTAACGAACAATCA+GGG + chr5_4:64867010-64867029 Msa0864480:three_prime_UTR 30.0%
ACTTTATCAGATGTATAAGC+AGG + chr5_4:64867070-64867089 Msa0864480:three_prime_UTR 30.0%
AAGCCTTGCAACAAAAAATA+TGG - chr5_4:64867145-64867164 None:intergenic 30.0%
CACCTACATAAAAGTTAGAA+AGG - chr5_4:64867180-64867199 None:intergenic 30.0%
ATCCATTATCCTGTTTCATA+TGG + chr5_4:64867268-64867287 Msa0864480:three_prime_UTR 30.0%
AACCATATGAAACAGGATAA+TGG - chr5_4:64867273-64867292 None:intergenic 30.0%
TTTGTGGACTTCGATATGTT+TGG - chr5_4:64864601-64864620 None:intergenic 35.0%
! AAGTAACGATTTGAAGTACG+AGG + chr5_4:64864714-64864733 Msa0864480:CDS 35.0%
ATAAGAGAGATCTCCAATTG+AGG - chr5_4:64864895-64864914 None:intergenic 35.0%
ATTGGAGATCTCTCTTATTC+TGG + chr5_4:64864897-64864916 Msa0864480:CDS 35.0%
TTCATCTCTTTCTCTCTCTT+AGG + chr5_4:64864924-64864943 Msa0864480:intron 35.0%
TCATCTCTTTCTCTCTCTTA+GGG + chr5_4:64864925-64864944 Msa0864480:intron 35.0%
!! TCTCTCTTAGGGTTTTATCA+TGG + chr5_4:64864936-64864955 Msa0864480:intron 35.0%
!! AGCAGTTAACAATTTTGGGT+CGG - chr5_4:64864979-64864998 None:intergenic 35.0%
!! GGGTTGATCTTTATTTCTGT+TGG + chr5_4:64865052-64865071 Msa0864480:intron 35.0%
!! ACATTGACAATTTTGATCGG+AGG + chr5_4:64865125-64865144 Msa0864480:intron 35.0%
TTGAGTGTAAACACATGTGT+CGG - chr5_4:64865169-64865188 None:intergenic 35.0%
CAACATGTGAGTGTTATGTT+TGG + chr5_4:64865299-64865318 Msa0864480:intron 35.0%
ATTGTCTGTGTGATAGATAG+AGG + chr5_4:64865392-64865411 Msa0864480:intron 35.0%
! GAGGAATACTTTTCTACCAA+AGG + chr5_4:64865411-64865430 Msa0864480:CDS 35.0%
!! GTTATTTTCATGCCAATGAG+AGG + chr5_4:64865448-64865467 Msa0864480:CDS 35.0%
AAAATCATCAGACCTCTCAT+TGG - chr5_4:64865463-64865482 None:intergenic 35.0%
AGTAAATATAGAATTCGGGG+TGG + chr5_4:64865920-64865939 Msa0864480:intron 35.0%
GGTGGATGAGAATCAAATTT+GGG + chr5_4:64865938-64865957 Msa0864480:intron 35.0%
GTGGATGAGAATCAAATTTG+GGG + chr5_4:64865939-64865958 Msa0864480:intron 35.0%
TGGATGAGAATCAAATTTGG+GGG + chr5_4:64865940-64865959 Msa0864480:intron 35.0%
ACATTGTCAAATGCAAAAGG+AGG - chr5_4:64866092-64866111 None:intergenic 35.0%
CAGACATTGTCAAATGCAAA+AGG - chr5_4:64866095-64866114 None:intergenic 35.0%
! CTTTTGCATTTGACAATGTC+TGG + chr5_4:64866093-64866112 Msa0864480:intron 35.0%
ACTTATGCTGAAAATAGGAG+GGG - chr5_4:64866193-64866212 None:intergenic 35.0%
GACTTATGCTGAAAATAGGA+GGG - chr5_4:64866194-64866213 None:intergenic 35.0%
TGACTTATGCTGAAAATAGG+AGG - chr5_4:64866195-64866214 None:intergenic 35.0%
GATCAATATACTGCAATGTG+TGG - chr5_4:64866247-64866266 None:intergenic 35.0%
CATTGGATGAAACCTTATAG+TGG - chr5_4:64866354-64866373 None:intergenic 35.0%
GGAATTAAGGCAAGTATTCA+GGG - chr5_4:64866418-64866437 None:intergenic 35.0%
AAAGGAAAGGAAAATAGGAG+GGG - chr5_4:64866442-64866461 None:intergenic 35.0%
GAAAGGAAAGGAAAATAGGA+GGG - chr5_4:64866443-64866462 None:intergenic 35.0%
CCTACTTGCTTATATCATCA+AGG - chr5_4:64866771-64866790 None:intergenic 35.0%
! CCTTGATGATATAAGCAAGT+AGG + chr5_4:64866768-64866787 Msa0864480:intron 35.0%
AAAAAGATTGGGAGAACGAA+GGG - chr5_4:64866811-64866830 None:intergenic 35.0%
AAAAAAGATTGGGAGAACGA+AGG - chr5_4:64866812-64866831 None:intergenic 35.0%
CCAATCCCTGAAAACAAAAT+TGG - chr5_4:64866888-64866907 None:intergenic 35.0%
!!! CCAATTTTGTTTTCAGGGAT+TGG + chr5_4:64866885-64866904 Msa0864480:intron 35.0%
! CAAAGTAGGTTGGATACAAA+TGG - chr5_4:64866949-64866968 None:intergenic 35.0%
ACAAAAAATATGGCCCTTTG+TGG - chr5_4:64867135-64867154 None:intergenic 35.0%
CTTAGCAAACCATATGAAAC+AGG - chr5_4:64867280-64867299 None:intergenic 35.0%
!! CTGTTTCATATGGTTTGCTA+AGG + chr5_4:64867278-64867297 Msa0864480:three_prime_UTR 35.0%
ATCATCCATCATCAGAAGCA+TGG + chr5_4:64864690-64864709 Msa0864480:exon 40.0%
! TCATCCATCATCAGAAGCAT+GGG + chr5_4:64864691-64864710 Msa0864480:exon 40.0%
GGATTTGAGAGTGGAAAAGA+GGG - chr5_4:64864864-64864883 None:intergenic 40.0%
AATCCCTCTTCTTCCTCAAT+TGG + chr5_4:64864879-64864898 Msa0864480:CDS 40.0%
TCTCCAATTGAGGAAGAAGA+GGG - chr5_4:64864885-64864904 None:intergenic 40.0%
ATCTCCAATTGAGGAAGAAG+AGG - chr5_4:64864886-64864905 None:intergenic 40.0%
GAGATCTCTCTTATTCTGGT+TGG + chr5_4:64864901-64864920 Msa0864480:intron 40.0%
ACCAAAGGGTTGAACATAGT+TGG + chr5_4:64865426-64865445 Msa0864480:CDS 40.0%
ACCAACTATGTTCAACCCTT+TGG - chr5_4:64865430-64865449 None:intergenic 40.0%
! AGGTCTGATGATTTTGAGCT+TGG + chr5_4:64865468-64865487 Msa0864480:CDS 40.0%
! TCTGATGATTTTGAGCTTGG+TGG + chr5_4:64865471-64865490 Msa0864480:CDS 40.0%
GTGGAAAGTAACGACAGATA+TGG - chr5_4:64865518-64865537 None:intergenic 40.0%
TCCACAAGCTGCTATTCTAT+TGG + chr5_4:64865533-64865552 Msa0864480:CDS 40.0%
ACCAATAGAATAGCAGCTTG+TGG - chr5_4:64865537-64865556 None:intergenic 40.0%
!! TGTTTAATCAAGGGAGCGTT+TGG + chr5_4:64865785-64865804 Msa0864480:intron 40.0%
!! GTTTAATCAAGGGAGCGTTT+GGG + chr5_4:64865786-64865805 Msa0864480:intron 40.0%
GGGTGGATGAGAATCAAATT+TGG + chr5_4:64865937-64865956 Msa0864480:intron 40.0%
! ATTTGGGGGTGAATTTTGTC+TGG + chr5_4:64865954-64865973 Msa0864480:intron 40.0%
! TGGCAAGTCTTATAATCACG+AGG + chr5_4:64866151-64866170 Msa0864480:intron 40.0%
CTTATGCTGAAAATAGGAGG+GGG - chr5_4:64866192-64866211 None:intergenic 40.0%
ATTCAGGGCTATATGGAAGA+CGG - chr5_4:64866403-64866422 None:intergenic 40.0%
GGGAATTAAGGCAAGTATTC+AGG - chr5_4:64866419-64866438 None:intergenic 40.0%
AAGGAAAGGAAAATAGGAGG+GGG - chr5_4:64866441-64866460 None:intergenic 40.0%
GGAAAGGAAAGGAAAATAGG+AGG - chr5_4:64866444-64866463 None:intergenic 40.0%
TTAGGGATGCATCTAAGAAC+TGG + chr5_4:64866626-64866645 Msa0864480:CDS 40.0%
TGCATCTAAGAACTGGAAGT+TGG + chr5_4:64866633-64866652 Msa0864480:CDS 40.0%
! ATGGCTCTTTGAGAGAGAAT+CGG - chr5_4:64866678-64866697 None:intergenic 40.0%
!! GTTGGATACAAATGGACTGA+GGG - chr5_4:64866941-64866960 None:intergenic 40.0%
TATCCAACCTACTTTGCAAG+TGG + chr5_4:64866953-64866972 Msa0864480:three_prime_UTR 40.0%
ATCCAACCTACTTTGCAAGT+GGG + chr5_4:64866954-64866973 Msa0864480:three_prime_UTR 40.0%
GGTGATGTTCAAGCATATCA+TGG + chr5_4:64866975-64866994 Msa0864480:three_prime_UTR 40.0%
! AGGGAATAGATCTTGACTTG+AGG + chr5_4:64867029-64867048 Msa0864480:three_prime_UTR 40.0%
! TCAGAGTTTTCCTTCCACAA+AGG + chr5_4:64867118-64867137 Msa0864480:three_prime_UTR 40.0%
! CAGAGTTTTCCTTCCACAAA+GGG + chr5_4:64867119-64867138 Msa0864480:three_prime_UTR 40.0%
AAAATATGGCCCTTTGTGGA+AGG - chr5_4:64867131-64867150 None:intergenic 40.0%
!!! GGGCCATATTTTTTGTTGCA+AGG + chr5_4:64867139-64867158 Msa0864480:three_prime_UTR 40.0%
! TGGAGTTTTCTTGCTTTGCT+TGG + chr5_4:64867208-64867227 Msa0864480:three_prime_UTR 40.0%
ACTTCCCATGCTTCTGATGA+TGG - chr5_4:64864698-64864717 None:intergenic 45.0%
TTGAGAGTGGAAAAGAGGGA+CGG - chr5_4:64864860-64864879 None:intergenic 45.0%
GGGATTTGAGAGTGGAAAAG+AGG - chr5_4:64864865-64864884 None:intergenic 45.0%
GAAGAAGAGGGATTTGAGAG+TGG - chr5_4:64864873-64864892 None:intergenic 45.0%
! CATAGTTGTTGGTGTCCTGT+TGG - chr5_4:64865244-64865263 None:intergenic 45.0%
!!! TGATTTTGAGCTTGGTGGTG+TGG + chr5_4:64865476-64865495 Msa0864480:CDS 45.0%
GAGTAGCAGTGCTATAATGC+AGG + chr5_4:64865678-64865697 Msa0864480:CDS 45.0%
GGAGGGGGAATTAAGTATTC+AGG - chr5_4:64866177-64866196 None:intergenic 45.0%
GGCAAGTATTCAGGGCTATA+TGG - chr5_4:64866410-64866429 None:intergenic 45.0%
AAATAGGAGGGGGGGAATTA+AGG - chr5_4:64866431-64866450 None:intergenic 45.0%
AGGAAAGGAAAATAGGAGGG+GGG - chr5_4:64866440-64866459 None:intergenic 45.0%
!! GGTTGGATACAAATGGACTG+AGG - chr5_4:64866942-64866961 None:intergenic 45.0%
ACATCACCCACTTGCAAAGT+AGG - chr5_4:64866963-64866982 None:intergenic 45.0%
!!! TTTATTTTAATAACAAAAAA+AGG - chr5_4:64866465-64866484 None:intergenic 5.0%
CAACACAGACAGTCACACGA+AGG - chr5_4:64864661-64864680 None:intergenic 50.0%
TGTCGTTAGAGGAGGAGATG+CGG - chr5_4:64864819-64864838 None:intergenic 50.0%
!! GATTTCGACGGTGTCGTTAG+AGG - chr5_4:64864830-64864849 None:intergenic 50.0%
!! CATGTGTCGGTGTCGAACAA+AGG - chr5_4:64865156-64865175 None:intergenic 50.0%
GTGTTGTGTTCGACACCAAC+AGG + chr5_4:64865226-64865245 Msa0864480:intron 50.0%
GGTGGTGTGGCAAAGAACAT+TGG + chr5_4:64865489-64865508 Msa0864480:CDS 50.0%
CTGCGCAGGATAAGTCCTAA+AGG - chr5_4:64866001-64866020 None:intergenic 50.0%
AGGAGTTCTTTGATCTGCGC+AGG - chr5_4:64866015-64866034 None:intergenic 50.0%
GGAAAGGAAAATAGGAGGGG+GGG - chr5_4:64866439-64866458 None:intergenic 50.0%
AAGATTGGGAGAACGAAGGG+TGG - chr5_4:64866808-64866827 None:intergenic 50.0%
!! TTCAGGGATTGGCTAAACCC+AGG + chr5_4:64866896-64866915 Msa0864480:intron 50.0%
!! TCAGGGATTGGCTAAACCCA+GGG + chr5_4:64866897-64866916 Msa0864480:intron 50.0%
GAGATCAGTTGAAGAGCCCT+GGG - chr5_4:64866916-64866935 None:intergenic 50.0%
CAGGGCTCTTCAACTGATCT+CGG + chr5_4:64866915-64866934 Msa0864480:exon 50.0%
CACCCACTTGCAAAGTAGGT+TGG - chr5_4:64866959-64866978 None:intergenic 50.0%
!! TTCGACGGTGTCGTTAGAGG+AGG - chr5_4:64864827-64864846 None:intergenic 55.0%
!! GACGGCATCGGTGATTTCGA+CGG - chr5_4:64864842-64864861 None:intergenic 55.0%
GTGGAAAAGAGGGACGGCAT+CGG - chr5_4:64864854-64864873 None:intergenic 55.0%
GGGCTATATGGAAGACGGCA+TGG - chr5_4:64866398-64866417 None:intergenic 55.0%
ATTGGGAGAACGAAGGGTGG+CGG - chr5_4:64866805-64866824 None:intergenic 55.0%
CGAGATCAGTTGAAGAGCCC+TGG - chr5_4:64866917-64866936 None:intergenic 55.0%
CACCCTACATCCGCCGTCAA+CGG + chr5_4:64864781-64864800 Msa0864480:CDS 60.0%
GATCAAGACGCCGTTGACGG+CGG - chr5_4:64864794-64864813 None:intergenic 60.0%
GCCGTCAACGGCGTCTTGAT+CGG + chr5_4:64864793-64864812 Msa0864480:CDS 60.0%
GCCGATCAAGACGCCGTTGA+CGG - chr5_4:64864797-64864816 None:intergenic 60.0%
CGCCGTTGACGGCGGATGTA+GGG - chr5_4:64864786-64864805 None:intergenic 65.0%
ACGCCGTTGACGGCGGATGT+AGG - chr5_4:64864787-64864806 None:intergenic 65.0%
! GGGAGAACGAAGGGTGGCGG+TGG - chr5_4:64866802-64866821 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 64864583 64867361 64864583 ID=Msa0864480;Name=Msa0864480
chr5_4 mRNA 64864583 64867361 64864583 ID=Msa0864480-mRNA-1;Parent=Msa0864480;Name=Msa0864480-mRNA-1;_AED=0.01;_eAED=0.01;_QI=126|1|1|1|1|1|5|430|204
chr5_4 exon 64864583 64864918 64864583 ID=Msa0864480-mRNA-1:exon:20386;Parent=Msa0864480-mRNA-1
chr5_4 exon 64865408 64865554 64865408 ID=Msa0864480-mRNA-1:exon:20387;Parent=Msa0864480-mRNA-1
chr5_4 exon 64865640 64865699 64865640 ID=Msa0864480-mRNA-1:exon:20388;Parent=Msa0864480-mRNA-1
chr5_4 exon 64866619 64866785 64866619 ID=Msa0864480-mRNA-1:exon:20389;Parent=Msa0864480-mRNA-1
chr5_4 exon 64866901 64867361 64866901 ID=Msa0864480-mRNA-1:exon:20390;Parent=Msa0864480-mRNA-1
chr5_4 five_prime_UTR 64864583 64864708 64864583 ID=Msa0864480-mRNA-1:five_prime_utr;Parent=Msa0864480-mRNA-1
chr5_4 CDS 64864709 64864918 64864709 ID=Msa0864480-mRNA-1:cds;Parent=Msa0864480-mRNA-1
chr5_4 CDS 64865408 64865554 64865408 ID=Msa0864480-mRNA-1:cds;Parent=Msa0864480-mRNA-1
chr5_4 CDS 64865640 64865699 64865640 ID=Msa0864480-mRNA-1:cds;Parent=Msa0864480-mRNA-1
chr5_4 CDS 64866619 64866785 64866619 ID=Msa0864480-mRNA-1:cds;Parent=Msa0864480-mRNA-1
chr5_4 CDS 64866901 64866931 64866901 ID=Msa0864480-mRNA-1:cds;Parent=Msa0864480-mRNA-1
chr5_4 three_prime_UTR 64866932 64867361 64866932 ID=Msa0864480-mRNA-1:three_prime_utr;Parent=Msa0864480-mRNA-1
Gene Sequence

>Msa0864480

ATGGGAAGTAACGATTTGAAGTACGAGGTTTCACAAAACGCATACATCAAACTCGTTCTTCACTCTCTCAAACACCCTACATCCGCCGTCAACGGCGTCTTGATCGGCCGCATCTCCTCCTCTAACGACACCGTCGAAATCACCGATGCCGTCCCTCTTTTCCACTCTCAAATCCCTCTTCTTCCTCAATTGGAGATCTCTCTTATTCTGATAGAGGAATACTTTTCTACCAAAGGGTTGAACATAGTTGGTTATTTTCATGCCAATGAGAGGTCTGATGATTTTGAGCTTGGTGGTGTGGCAAAGAACATTGGTGACCATATCTGTCGTTACTTTCCACAAGCTGCTATTCTATTGTTAGATAACAAGAAGCTTGAAGCTTTGAAAAAGAGCAAGAGTAGCAGTGCTATAATGCAGCTTTATGTTAGGGATGCATCTAAGAACTGGAAGTTGGTTCAGTCAGATGCGAACAACCGATTCTCTCTCAAAGAGCCATCAGCAAATGTGATCTTATTGGATTATATTTCATCTGAGAAATGGAATGACATTGTAGATTTTGATGATCACCTTGATGATATAAGCAAGGATTGGCTAAACCCAGGGCTCTTCAACTGA
Protein sequence

>Msa0864480

MGSNDLKYEVSQNAYIKLVLHSLKHPTSAVNGVLIGRISSSNDTVEITDAVPLFHSQIPLLPQLEISLILIEEYFSTKGLNIVGYFHANERSDDFELGGVAKNIGDHICRYFPQAAILLLDNKKLEALKKSKSSSAIMQLYVRDASKNWKLVQSDANNRFSLKEPSANVILLDYISSEKWNDIVDFDDHLDDISKDWLNPGLFN*