AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0970110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970110 MtrunA17_Chr6g0448891 96.825 126 4 0 1 126 1 126 6.44e-85 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970110 AT1G08580.1 54.348 92 41 1 1 92 1 91 5.50e-28 99.8

Find 39 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATAAAATATTATTTC+TGG 0.157938 6_4:+1595247 Msa0970110:three_prime_UTR
TGAGGTTCAGAGAGTGAATT+AGG 0.175801 6_4:+1592742 Msa0970110:five_prime_UTR
CTTTGTCCTTTACTTTATAT+TGG 0.277243 6_4:+1595181 Msa0970110:three_prime_UTR
CTTTCTGATCTTAAGCTTGC+CGG 0.286439 6_4:-1592868 None:intergenic
TAACCTTGAATTGAGTGTTT+TGG 0.289455 6_4:+1594980 Msa0970110:three_prime_UTR
AGATGCAGCACTCTTATCTT+TGG 0.304089 6_4:-1593217 None:intergenic
AAATCAGTGAGCTTCCTTCT+AGG 0.306730 6_4:-1595104 None:intergenic
GAGCTTGCGCTTGCGTTTGC+CGG 0.309830 6_4:-1592907 None:intergenic
CGTTTGCCGGATAGATTAAC+AGG 0.331339 6_4:-1592894 None:intergenic
CTTCATCTAACATTTGATAA+AGG 0.333342 6_4:-1595137 None:intergenic
CTTCTATCCTCATACTATAA+AGG 0.366513 6_4:-1594930 None:intergenic
CAAAAGAAAATCTGATGTTC+CGG 0.399282 6_4:+1594860 Msa0970110:CDS
GAGCATAAAATATTATTTCT+GGG 0.417415 6_4:+1595248 Msa0970110:three_prime_UTR
ACCCCAAAACACTCAATTCA+AGG 0.443624 6_4:-1594983 None:intergenic
GAGGTTCAGAGAGTGAATTA+GGG 0.459949 6_4:+1592743 Msa0970110:five_prime_UTR
AAGGAAATCAAATTAGCATA+TGG 0.462611 6_4:+1595207 Msa0970110:three_prime_UTR
AGTAAAGGACAAAGATAAAC+CGG 0.475592 6_4:-1595172 None:intergenic
ATCTTAAGCTTGCCGGTGGT+AGG 0.480726 6_4:-1592861 None:intergenic
ATTGTTGTCATATTCCTAGA+AGG 0.483581 6_4:+1595090 Msa0970110:three_prime_UTR
AAGCAGCCTGTTAATCTATC+CGG 0.484871 6_4:+1592888 Msa0970110:CDS
TTTGAGGGAGGAGGCAGGGA+TGG 0.490322 6_4:+1592767 Msa0970110:exon
CTTTACTTTATATTGGTGAA+AGG 0.493663 6_4:+1595188 Msa0970110:three_prime_UTR
CTTTCACCAATATAAAGTAA+AGG 0.505194 6_4:-1595187 None:intergenic
CTTGAAACTTAAACAGCTCA+AGG 0.522552 6_4:+1593269 Msa0970110:CDS
TCTTAAGCTTGCCGGTGGTA+GGG 0.536083 6_4:-1592860 None:intergenic
TAGCATGGAAGATGTTCAAA+TGG 0.542838 6_4:+1593068 Msa0970110:CDS
TCAGTTGAAACTTCAGCAGC+CGG 0.558824 6_4:-1594879 None:intergenic
CTTAAACAGCTCAAGGGCAA+AGG 0.565635 6_4:+1593276 Msa0970110:CDS
GAGAGGAAGAGATTGATTCA+CGG 0.579715 6_4:+1592834 Msa0970110:CDS
AGAGAGTGGCTTGATTAGCA+TGG 0.596496 6_4:+1593053 Msa0970110:CDS
GATGAAGTAGTATATTGCAC+CGG 0.608910 6_4:+1595153 Msa0970110:three_prime_UTR
AGCGCAAGCTCTTCAAACAA+TGG 0.614028 6_4:+1592919 Msa0970110:CDS
TGAAGTTTCAACTGATGCAA+TGG 0.630524 6_4:+1594887 Msa0970110:CDS
GTTCAAATGGCTGTTGCTGA+AGG 0.631234 6_4:+1593081 Msa0970110:CDS
TTATAGTATGAGGATAGAAG+AGG 0.639645 6_4:+1594933 Msa0970110:three_prime_UTR
AGTTTCAACTGATGCAATGG+TGG 0.646685 6_4:+1594890 Msa0970110:CDS
GAAGAGCTCTAGTTGCTGAG+AGG 0.671480 6_4:+1592817 Msa0970110:CDS
TTGAAACTTAAACAGCTCAA+GGG 0.676667 6_4:+1593270 Msa0970110:CDS
TCTGATCTTAAGCTTGCCGG+TGG 0.687271 6_4:-1592865 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAATATAAATAAAA+AGG - chr6_4:1595027-1595046 None:intergenic 0.0%
!!! TTTTTATTTATATTTTTTTA+TGG + chr6_4:1595026-1595045 Msa0970110:three_prime_UTR 0.0%
!! TAAATTTCACTTGAAAAAAA+AGG + chr6_4:1593245-1593264 Msa0970110:CDS 15.0%
!! AAATTTCACTTGAAAAAAAA+GGG + chr6_4:1593246-1593265 Msa0970110:CDS 15.0%
!!! AAAACTAATTTCTAGTTATA+AGG - chr6_4:1593516-1593535 None:intergenic 15.0%
!!! ATCTGCTTTTAAATATATTT+TGG + chr6_4:1593739-1593758 Msa0970110:intron 15.0%
!!! TTTTAAATATATTTTGGAGT+TGG + chr6_4:1593745-1593764 Msa0970110:intron 15.0%
!!! AGTTGATTTATTTTTTTAAG+TGG + chr6_4:1593846-1593865 Msa0970110:intron 15.0%
!! ACATATAATTCAAAAAGAAA+GGG - chr6_4:1594328-1594347 None:intergenic 15.0%
!! AACATATAATTCAAAAAGAA+AGG - chr6_4:1594329-1594348 None:intergenic 15.0%
!!! ATATTTTGTAAACAATTTGA+TGG - chr6_4:1594790-1594809 None:intergenic 15.0%
!!! ATTTTTTTATGGTAAAAGAA+TGG + chr6_4:1595037-1595056 Msa0970110:three_prime_UTR 15.0%
!! CAACAATAATATAATGTTTG+TGG + chr6_4:1593651-1593670 Msa0970110:intron 20.0%
!!! ATTTTTTTAAGTGGTATCTT+TGG + chr6_4:1593855-1593874 Msa0970110:intron 20.0%
!!! AGTTTAAAATGATTGAAAGT+AGG - chr6_4:1594485-1594504 None:intergenic 20.0%
!!! TTTTGTATAAATATGTCTAC+TGG - chr6_4:1594583-1594602 None:intergenic 20.0%
!!! TTTCACTTTCAATTTGATTT+TGG - chr6_4:1594611-1594630 None:intergenic 20.0%
!! AAATAAAACCTGTTACTATT+TGG - chr6_4:1594643-1594662 None:intergenic 20.0%
!!! ATGTTTTGAATATAGACATA+TGG + chr6_4:1594687-1594706 Msa0970110:intron 20.0%
!!! TGTTTTGAATATAGACATAT+GGG + chr6_4:1594688-1594707 Msa0970110:intron 20.0%
!!! ATTTTTTGAATTTCTTGTTG+TGG + chr6_4:1594745-1594764 Msa0970110:intron 20.0%
!!! TTCACTTTTTGATTAACTGA+AGG + chr6_4:1593014-1593033 Msa0970110:intron 25.0%
! GGAAACTGAAATTCATTTAA+GGG - chr6_4:1593125-1593144 None:intergenic 25.0%
! AGGAAACTGAAATTCATTTA+AGG - chr6_4:1593126-1593145 None:intergenic 25.0%
! AAAAGAGCTCATTTATTGTT+TGG + chr6_4:1593176-1593195 Msa0970110:intron 25.0%
!!! GGCAAAGGTAATTTTTTTTT+TGG + chr6_4:1593291-1593310 Msa0970110:intron 25.0%
!!! ATTTTGACTACCTTGATTAT+TGG - chr6_4:1593556-1593575 None:intergenic 25.0%
! TCAAAATTGAGATTCTATCT+AGG + chr6_4:1593569-1593588 Msa0970110:intron 25.0%
! CAAAATTGAGATTCTATCTA+GGG + chr6_4:1593570-1593589 Msa0970110:intron 25.0%
! CAATAATATAATGTTTGTGG+TGG + chr6_4:1593654-1593673 Msa0970110:intron 25.0%
!! CTTATCAATTGTTGTTGATA+AGG + chr6_4:1593680-1593699 Msa0970110:intron 25.0%
! TTCAAAACAACGATTAATCT+AGG + chr6_4:1593898-1593917 Msa0970110:intron 25.0%
!!! GATTTTTGACTATCAAAACA+AGG - chr6_4:1594005-1594024 None:intergenic 25.0%
! AAAAAGAAAATGCAGAAACT+AGG + chr6_4:1594179-1594198 Msa0970110:intron 25.0%
!!! ATTCACTTTTAAGATTGTAG+TGG + chr6_4:1594527-1594546 Msa0970110:intron 25.0%
! CTTCATCTAACATTTGATAA+AGG - chr6_4:1595140-1595159 None:intergenic 25.0%
! CTTTGTCCTTTACTTTATAT+TGG + chr6_4:1595181-1595200 Msa0970110:three_prime_UTR 25.0%
! CTTTCACCAATATAAAGTAA+AGG - chr6_4:1595190-1595209 None:intergenic 25.0%
! CTTTACTTTATATTGGTGAA+AGG + chr6_4:1595188-1595207 Msa0970110:three_prime_UTR 25.0%
! AAGGAAATCAAATTAGCATA+TGG + chr6_4:1595207-1595226 Msa0970110:three_prime_UTR 25.0%
! GAGCTCTTTTGTTAAACTAA+AGG - chr6_4:1593166-1593185 None:intergenic 30.0%
TTGAAACTTAAACAGCTCAA+GGG + chr6_4:1593270-1593289 Msa0970110:CDS 30.0%
!!! TTTTGGTTCTGTTTTGCATT+TGG + chr6_4:1593308-1593327 Msa0970110:intron 30.0%
!! TCAAATAATTATTGGTGTCG+AGG - chr6_4:1593363-1593382 None:intergenic 30.0%
AGGACTCAATTGTAAATCAT+GGG + chr6_4:1593600-1593619 Msa0970110:intron 30.0%
TAATATAATGTTTGTGGTGG+AGG + chr6_4:1593657-1593676 Msa0970110:intron 30.0%
TGTGATATCTAAATGAACGA+GGG - chr6_4:1593780-1593799 None:intergenic 30.0%
ATGTGATATCTAAATGAACG+AGG - chr6_4:1593781-1593800 None:intergenic 30.0%
ATATCACATGATATAGCCAA+GGG + chr6_4:1593792-1593811 Msa0970110:intron 30.0%
! AACGATTAATCTAGGATTCT+TGG + chr6_4:1593906-1593925 Msa0970110:intron 30.0%
TAGCCACAAACATTAGAATA+GGG - chr6_4:1594102-1594121 None:intergenic 30.0%
TTAGCCACAAACATTAGAAT+AGG - chr6_4:1594103-1594122 None:intergenic 30.0%
TATTCTAATGTTTGTGGCTA+AGG + chr6_4:1594102-1594121 Msa0970110:intron 30.0%
GAGAATAAATCTGTTACAGA+GGG - chr6_4:1594306-1594325 None:intergenic 30.0%
! CTGTTACTATTTGGTTGATA+AGG - chr6_4:1594634-1594653 None:intergenic 30.0%
CTTATCAACCAAATAGTAAC+AGG + chr6_4:1594632-1594651 Msa0970110:intron 30.0%
CTGCGTAGTTAACTTATAAT+TGG + chr6_4:1594811-1594830 Msa0970110:intron 30.0%
! CTTATAATTGGTGTTTGTTG+TGG + chr6_4:1594823-1594842 Msa0970110:intron 30.0%
CAAAAGAAAATCTGATGTTC+CGG + chr6_4:1594860-1594879 Msa0970110:CDS 30.0%
! GTTTTCTCCTTTATAGTATG+AGG + chr6_4:1594923-1594942 Msa0970110:three_prime_UTR 30.0%
CTTCTATCCTCATACTATAA+AGG - chr6_4:1594933-1594952 None:intergenic 30.0%
TTATAGTATGAGGATAGAAG+AGG + chr6_4:1594933-1594952 Msa0970110:three_prime_UTR 30.0%
! TAACCTTGAATTGAGTGTTT+TGG + chr6_4:1594980-1594999 Msa0970110:three_prime_UTR 30.0%
! AACCTTGAATTGAGTGTTTT+GGG + chr6_4:1594981-1595000 Msa0970110:three_prime_UTR 30.0%
ATTGTTGTCATATTCCTAGA+AGG + chr6_4:1595090-1595109 Msa0970110:three_prime_UTR 30.0%
AGTAAAGGACAAAGATAAAC+CGG - chr6_4:1595175-1595194 None:intergenic 30.0%
!! AGAGTGAATTAGGGTTTTGA+GGG + chr6_4:1592752-1592771 Msa0970110:five_prime_UTR 35.0%
TAGCATGGAAGATGTTCAAA+TGG + chr6_4:1593068-1593087 Msa0970110:CDS 35.0%
!!! TATTGTTTGGTTTTGTGAGC+AGG + chr6_4:1593189-1593208 Msa0970110:intron 35.0%
!!! ATTGTTTGGTTTTGTGAGCA+GGG + chr6_4:1593190-1593209 Msa0970110:intron 35.0%
CTTGAAACTTAAACAGCTCA+AGG + chr6_4:1593269-1593288 Msa0970110:CDS 35.0%
AAGGAAATTTGAATCAAGCC+AGG - chr6_4:1593497-1593516 None:intergenic 35.0%
! TGTTTGCTGACCAATAATCA+AGG + chr6_4:1593543-1593562 Msa0970110:intron 35.0%
GAGGACTCAATTGTAAATCA+TGG + chr6_4:1593599-1593618 Msa0970110:intron 35.0%
GATATCACATGATATAGCCA+AGG + chr6_4:1593791-1593810 Msa0970110:intron 35.0%
TATCACATGATATAGCCAAG+GGG + chr6_4:1593793-1593812 Msa0970110:intron 35.0%
!! GGGAAGTGTCAATGTTAATT+TGG + chr6_4:1593935-1593954 Msa0970110:intron 35.0%
AACATTAGAATAGGGACCAA+AGG - chr6_4:1594094-1594113 None:intergenic 35.0%
CTATACGCTGAAACTGAAAA+TGG + chr6_4:1594244-1594263 Msa0970110:intron 35.0%
GGAGAATAAATCTGTTACAG+AGG - chr6_4:1594307-1594326 None:intergenic 35.0%
AGTAGATTTCAGCTTCTCAT+TGG + chr6_4:1594449-1594468 Msa0970110:intron 35.0%
!! TCTACTGGTTCTACATGTTA+TGG - chr6_4:1594568-1594587 None:intergenic 35.0%
! CGGAACATCAGATTTTCTTT+TGG - chr6_4:1594862-1594881 None:intergenic 35.0%
TGAAGTTTCAACTGATGCAA+TGG + chr6_4:1594887-1594906 Msa0970110:CDS 35.0%
TAGAAGAGGCAAAAATGAGA+TGG + chr6_4:1594947-1594966 Msa0970110:three_prime_UTR 35.0%
! ACCTTGAATTGAGTGTTTTG+GGG + chr6_4:1594982-1595001 Msa0970110:three_prime_UTR 35.0%
GATGAAGTAGTATATTGCAC+CGG + chr6_4:1595153-1595172 Msa0970110:three_prime_UTR 35.0%
TGAGGTTCAGAGAGTGAATT+AGG + chr6_4:1592742-1592761 Msa0970110:five_prime_UTR 40.0%
GAGGTTCAGAGAGTGAATTA+GGG + chr6_4:1592743-1592762 Msa0970110:five_prime_UTR 40.0%
! GAGAGTGAATTAGGGTTTTG+AGG + chr6_4:1592751-1592770 Msa0970110:five_prime_UTR 40.0%
! GAGAGGAAGAGATTGATTCA+CGG + chr6_4:1592834-1592853 Msa0970110:CDS 40.0%
CTTTCTGATCTTAAGCTTGC+CGG - chr6_4:1592871-1592890 None:intergenic 40.0%
AAGCAGCCTGTTAATCTATC+CGG + chr6_4:1592888-1592907 Msa0970110:CDS 40.0%
CAAAAAGATGCTGTAGAGAG+TGG + chr6_4:1593039-1593058 Msa0970110:CDS 40.0%
!! TTTAAGGGATTGATAGCGCA+CGG - chr6_4:1593110-1593129 None:intergenic 40.0%
!!! TTGTTTGGTTTTGTGAGCAG+GGG + chr6_4:1593191-1593210 Msa0970110:intron 40.0%
AGATGCAGCACTCTTATCTT+TGG - chr6_4:1593220-1593239 None:intergenic 40.0%
!! GAGTTATGAAAGCACCGATA+TGG + chr6_4:1593334-1593353 Msa0970110:intron 40.0%
CTACATAGATTGAGTCTACC+TGG + chr6_4:1593476-1593495 Msa0970110:intron 40.0%
! ATTCTATCTAGGGTCAAGAG+AGG + chr6_4:1593580-1593599 Msa0970110:intron 40.0%
CATGGGATTGTAACATAGCA+TGG + chr6_4:1593617-1593636 Msa0970110:intron 40.0%
! AATCTAGGATTCTTGGATCG+AGG + chr6_4:1593913-1593932 Msa0970110:intron 40.0%
! ATCTAGGATTCTTGGATCGA+GGG + chr6_4:1593914-1593933 Msa0970110:intron 40.0%
CCATCACTCTTGAAGACTAA+TGG - chr6_4:1594034-1594053 None:intergenic 40.0%
!! CCATTAGTCTTCAAGAGTGA+TGG + chr6_4:1594031-1594050 Msa0970110:intron 40.0%
! TTAGTCTTCAAGAGTGATGG+TGG + chr6_4:1594034-1594053 Msa0970110:intron 40.0%
ATTAGAATAGGGACCAAAGG+AGG - chr6_4:1594091-1594110 None:intergenic 40.0%
GGTCCCTATTCTAATGTTTG+TGG + chr6_4:1594096-1594115 Msa0970110:intron 40.0%
TGTTACAGAGGGTAATTGAG+GGG - chr6_4:1594295-1594314 None:intergenic 40.0%
CTGTTACAGAGGGTAATTGA+GGG - chr6_4:1594296-1594315 None:intergenic 40.0%
TCTGTTACAGAGGGTAATTG+AGG - chr6_4:1594297-1594316 None:intergenic 40.0%
GTTGATGCAGTGATAACACA+TGG + chr6_4:1594346-1594365 Msa0970110:intron 40.0%
GATTTCAGCTTCTCATTGGA+TGG + chr6_4:1594453-1594472 Msa0970110:intron 40.0%
! TGGTTCTACATGTTATGGTG+CGG - chr6_4:1594563-1594582 None:intergenic 40.0%
!! TGGTGTTTGTTGTGGCATAT+AGG + chr6_4:1594831-1594850 Msa0970110:intron 40.0%
!! GGTGTTTGTTGTGGCATATA+GGG + chr6_4:1594832-1594851 Msa0970110:intron 40.0%
AGTTTCAACTGATGCAATGG+TGG + chr6_4:1594890-1594909 Msa0970110:CDS 40.0%
ACCCCAAAACACTCAATTCA+AGG - chr6_4:1594986-1595005 None:intergenic 40.0%
!!! TGAATTGAGTGTTTTGGGGT+AGG + chr6_4:1594986-1595005 Msa0970110:three_prime_UTR 40.0%
AAATCAGTGAGCTTCCTTCT+AGG - chr6_4:1595107-1595126 None:intergenic 40.0%
!!! GTGAATTAGGGTTTTGAGGG+AGG + chr6_4:1592755-1592774 Msa0970110:five_prime_UTR 45.0%
!!! AATTAGGGTTTTGAGGGAGG+AGG + chr6_4:1592758-1592777 Msa0970110:five_prime_UTR 45.0%
CGTTTGCCGGATAGATTAAC+AGG - chr6_4:1592897-1592916 None:intergenic 45.0%
AGCGCAAGCTCTTCAAACAA+TGG + chr6_4:1592919-1592938 Msa0970110:CDS 45.0%
AGAGAGTGGCTTGATTAGCA+TGG + chr6_4:1593053-1593072 Msa0970110:CDS 45.0%
GTTCAAATGGCTGTTGCTGA+AGG + chr6_4:1593081-1593100 Msa0970110:CDS 45.0%
AGGAATCAGCTAATCACTCG+AGG - chr6_4:1593146-1593165 None:intergenic 45.0%
CTTAAACAGCTCAAGGGCAA+AGG + chr6_4:1593276-1593295 Msa0970110:CDS 45.0%
CATGTGTACACTGACATGTG+TGG - chr6_4:1593416-1593435 None:intergenic 45.0%
CACACATGTCAGTGTACACA+TGG + chr6_4:1593414-1593433 Msa0970110:intron 45.0%
AGTGTACACATGGCTGACAT+TGG + chr6_4:1593424-1593443 Msa0970110:intron 45.0%
GTGTACACATGGCTGACATT+GGG + chr6_4:1593425-1593444 Msa0970110:intron 45.0%
! TCTAGGATTCTTGGATCGAG+GGG + chr6_4:1593915-1593934 Msa0970110:intron 45.0%
!! TCTTCAAGAGTGATGGTGGT+TGG + chr6_4:1594038-1594057 Msa0970110:intron 45.0%
CAACTATTAGCGACCTCCTT+TGG + chr6_4:1594075-1594094 Msa0970110:intron 45.0%
CAGTTTCAGCGTATAGCTTG+TGG - chr6_4:1594240-1594259 None:intergenic 45.0%
ACAAAGCAGCAAGAATGCTC+TGG - chr6_4:1594395-1594414 None:intergenic 45.0%
GTTCACAACAACGCAGTGTA+GGG - chr6_4:1594421-1594440 None:intergenic 45.0%
AGTTCACAACAACGCAGTGT+AGG - chr6_4:1594422-1594441 None:intergenic 45.0%
ACTGCGTTGTTGTGAACTAG+CGG + chr6_4:1594424-1594443 Msa0970110:intron 45.0%
TCTACATGTTATGGTGCGGT+AGG - chr6_4:1594559-1594578 None:intergenic 45.0%
TCAGTTGAAACTTCAGCAGC+CGG - chr6_4:1594882-1594901 None:intergenic 45.0%
!!! TTTTTTTTTCAAATAATTAT+TGG - chr6_4:1593371-1593390 None:intergenic 5.0%
! GAAGAGCTCTAGTTGCTGAG+AGG + chr6_4:1592817-1592836 Msa0970110:CDS 50.0%
TCTTAAGCTTGCCGGTGGTA+GGG - chr6_4:1592863-1592882 None:intergenic 50.0%
ATCTTAAGCTTGCCGGTGGT+AGG - chr6_4:1592864-1592883 None:intergenic 50.0%
TCTGATCTTAAGCTTGCCGG+TGG - chr6_4:1592868-1592887 None:intergenic 50.0%
TGTGGCTAAGGCTGCACATT+TGG + chr6_4:1594114-1594133 Msa0970110:intron 50.0%
ACAGAGGGTAATTGAGGGGA+GGG - chr6_4:1594291-1594310 None:intergenic 50.0%
TACAGAGGGTAATTGAGGGG+AGG - chr6_4:1594292-1594311 None:intergenic 50.0%
ATGGCATGCTACCAACTGAG+AGG + chr6_4:1594365-1594384 Msa0970110:intron 50.0%
! GCTCTGGTTTACCTCTCAGT+TGG - chr6_4:1594379-1594398 None:intergenic 50.0%
! TGGTGTCGAGGCGTCCATAT+CGG - chr6_4:1593351-1593370 None:intergenic 55.0%
ATTGGGCACACGCCATCGAT+CGG + chr6_4:1593442-1593461 Msa0970110:intron 55.0%
! ACGCCATCGATCGGAAGTGT+TGG + chr6_4:1593451-1593470 Msa0970110:intron 55.0%
GCACCAACACTTCCGATCGA+TGG - chr6_4:1593457-1593476 None:intergenic 55.0%
TGATATAGCCAAGGGGCGAG+TGG + chr6_4:1593800-1593819 Msa0970110:intron 55.0%
GATATAGCCAAGGGGCGAGT+GGG + chr6_4:1593801-1593820 Msa0970110:intron 55.0%
GGCGAGTGGGTCTCTACATA+GGG + chr6_4:1593814-1593833 Msa0970110:intron 55.0%
AGAGGGTAATTGAGGGGAGG+GGG - chr6_4:1594289-1594308 None:intergenic 55.0%
CAGAGGGTAATTGAGGGGAG+GGG - chr6_4:1594290-1594309 None:intergenic 55.0%
!!! AGGGTTTTGAGGGAGGAGGC+AGG + chr6_4:1592762-1592781 Msa0970110:five_prime_UTR 60.0%
!!! GGGTTTTGAGGGAGGAGGCA+GGG + chr6_4:1592763-1592782 Msa0970110:five_prime_UTR 60.0%
TTTGAGGGAGGAGGCAGGGA+TGG + chr6_4:1592767-1592786 Msa0970110:exon 60.0%
ATTCACGGCGACCCTACCAC+CGG + chr6_4:1592849-1592868 Msa0970110:CDS 60.0%
GAGCTTGCGCTTGCGTTTGC+CGG - chr6_4:1592910-1592929 None:intergenic 60.0%
GGGCGAGTGGGTCTCTACAT+AGG + chr6_4:1593813-1593832 Msa0970110:intron 60.0%
TAATTGAGGGGAGGGGGCTG+GGG - chr6_4:1594283-1594302 None:intergenic 60.0%
GTAATTGAGGGGAGGGGGCT+GGG - chr6_4:1594284-1594303 None:intergenic 60.0%
GTAGAGACCCACTCGCCCCT+TGG - chr6_4:1593811-1593830 None:intergenic 65.0%
GGTAATTGAGGGGAGGGGGC+TGG - chr6_4:1594285-1594304 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 1592741 1595268 1592741 ID=Msa0970110;Name=Msa0970110
chr6_4 mRNA 1592741 1595268 1592741 ID=Msa0970110-mRNA-1;Parent=Msa0970110;Name=Msa0970110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45|1|1|1|1|1|4|351|126
chr6_4 exon 1592741 1592947 1592741 ID=Msa0970110-mRNA-1:exon:805;Parent=Msa0970110-mRNA-1
chr6_4 exon 1593036 1593102 1593036 ID=Msa0970110-mRNA-1:exon:806;Parent=Msa0970110-mRNA-1
chr6_4 exon 1593211 1593297 1593211 ID=Msa0970110-mRNA-1:exon:807;Parent=Msa0970110-mRNA-1
chr6_4 exon 1594853 1595268 1594853 ID=Msa0970110-mRNA-1:exon:808;Parent=Msa0970110-mRNA-1
chr6_4 five_prime_UTR 1592741 1592785 1592741 ID=Msa0970110-mRNA-1:five_prime_utr;Parent=Msa0970110-mRNA-1
chr6_4 CDS 1592786 1592947 1592786 ID=Msa0970110-mRNA-1:cds;Parent=Msa0970110-mRNA-1
chr6_4 CDS 1593036 1593102 1593036 ID=Msa0970110-mRNA-1:cds;Parent=Msa0970110-mRNA-1
chr6_4 CDS 1593211 1593297 1593211 ID=Msa0970110-mRNA-1:cds;Parent=Msa0970110-mRNA-1
chr6_4 CDS 1594853 1594917 1594853 ID=Msa0970110-mRNA-1:cds;Parent=Msa0970110-mRNA-1
chr6_4 three_prime_UTR 1594918 1595268 1594918 ID=Msa0970110-mRNA-1:three_prime_utr;Parent=Msa0970110-mRNA-1
Gene Sequence

>Msa0970110

ATGGCGAAGTTCAACGTTGTTCAGAAAAGAAGAAGAGCTCTAGTTGCTGAGAGGAAGAGATTGATTCACGGCGACCCTACCACCGGCAAGCTTAAGATCAGAAAGCAGCCTGTTAATCTATCCGGCAAACGCAAGCGCAAGCTCTTCAAACAATGGCGTCGTGAACAAAAAGATGCTGTAGAGAGTGGCTTGATTAGCATGGAAGATGTTCAAATGGCTGTTGCTGAAGGGGAAACCAAAGATAAGAGTGCTGCATCTTCAGCTAAATTTCACTTGAAAAAAAAGGGCTTGAAACTTAAACAGCTCAAGGGCAAAGGGAAGACCAAAAGAAAATCTGATGTTCCGGCTGCTGAAGTTTCAACTGATGCAATGGTGGAGTAA
Protein sequence

>Msa0970110

MAKFNVVQKRRRALVAERKRLIHGDPTTGKLKIRKQPVNLSGKRKRKLFKQWRREQKDAVESGLISMEDVQMAVAEGETKDKSAASSAKFHLKKKGLKLKQLKGKGKTKRKSDVPAAEVSTDAMVE*