AlfalfaGEDB | Msa1047150

M. sativa cultivar zhongmu-4 / Msa1047150

KEGG: K10839
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0031593 GO:0032182 GO:0043130 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0050896 GO:0070628 GO:0140030
PFAM: PF00240.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1047150	XP_013447820.1	95.225	377	10	3	1	376	1	370	0.0	702

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1047150	sp\|Q84L30\|RD23D_ARATH	67.016	382	115	7	1	376	1	377	2.41e-161	460

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1047150	A0A072TVG7	95.225	377	10	3	1	376	1	370	0.0	702

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0038350	Msa1047150	0.821790	3.347386e-53	-8.615850e-47
Msa0039420	Msa1047150	0.806869	6.671358e-50	-8.615850e-47
Msa0185640	Msa1047150	0.814175	1.761252e-51	-8.615850e-47
Msa0200950	Msa1047150	0.820151	7.980590e-53	-8.615850e-47
Msa0258670	Msa1047150	0.818198	2.221104e-52	-8.615850e-47
Msa0312690	Msa1047150	0.806948	6.420507e-50	-8.615850e-47
Msa0342340	Msa1047150	0.814251	1.694859e-51	-8.615850e-47
Msa0467900	Msa1047150	0.808286	3.337549e-50	-8.615850e-47
Msa0657670	Msa1047150	-0.808134	3.595547e-50	-8.615850e-47
Msa1004900	Msa1047150	0.847628	1.025353e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1047150	MtrunA17_Chr7g0214981	95.225	377	10	3	1	376	1	370	0.0	702
Msa1047150	MtrunA17_Chr1g0209971	64.750	400	113	6	1	376	1	396	3.41e-166	470
Msa1047150	MtrunA17_Chr2g0334051	55.556	378	154	5	1	376	1	366	1.88e-130	378
Msa1047150	MtrunA17_Chr1g0146801	60.714	84	32	1	1	83	1	84	1.24e-25	100
Msa1047150	MtrunA17_Chr7g0226581	60.526	76	30	0	1	76	205	280	3.35e-24	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1047150	AT5G38470.1	67.016	382	115	7	1	376	1	377	2.45e-162	460
Msa1047150	AT3G02540.3	59.633	436	98	10	1	376	1	418	1.33e-161	459
Msa1047150	AT3G02540.1	60.859	419	121	8	1	376	1	419	2.83e-161	459
Msa1047150	AT1G79650.2	55.497	382	147	7	1	376	1	365	1.30e-133	387
Msa1047150	AT1G79650.1	54.522	387	149	7	1	376	1	371	6.56e-133	385
Msa1047150	AT1G79650.4	53.117	401	157	8	1	376	1	395	3.87e-131	381
Msa1047150	AT1G16190.1	50.758	396	147	6	1	376	1	368	3.78e-127	370
Msa1047150	AT5G38470.2	64.526	327	104	7	1	320	1	322	1.47e-124	362
Msa1047150	AT1G16190.2	50.135	371	137	6	26	376	3	345	4.34e-115	338
Msa1047150	AT1G79650.3	50.129	387	146	8	1	376	1	351	8.75e-115	338

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Find 128 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAATCCAAACCTAA+TGG	0.410836	7_2:-7861531	Msa1047150:CDS
AAAATTTGTACTTACCGGTG+GGG	0.638126	7_2:+7863468	None:intergenic
AAATGAAAATGCAGGTGACT+TGG	0.550169	7_2:-7861809	Msa1047150:CDS
AAATTAGAATTGTGTTGGGA+TGG	0.534851	7_2:-7860425	Msa1047150:three_prime_UTR
AACAGCAGGCACAGCTGGTT+GGG	0.370179	7_2:+7861977	None:intergenic
AACCAAGCTGAGAAGATTGC+GGG	0.400142	7_2:-7860315	None:intergenic
AACCAGCTGTGCCTGCTGTT+GGG	0.345512	7_2:-7861974	Msa1047150:CDS
AACCCGCAATCTTCTCAGCT+TGG	0.482634	7_2:+7860313	None:intergenic
AAGAGAACGAGGCCATTCAA+CGG	0.619236	7_2:-7861047	Msa1047150:CDS
AAGCACCCAACTGTCCCTCT+AGG	0.566429	7_2:+7861100	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAACGTGAAGACTCTTAA+GGG	-	chr7_2:7860442-7860461	Msa1047150:three_prime_UTR	30.0%
TTGAATGTAGTTACATGTGT+CGG	-	chr7_2:7860703-7860722	Msa1047150:CDS	30.0%
GTTCTTTAAACTAAACTGGA+AGG	+	chr7_2:7860811-7860830	None:intergenic	30.0%
CAAATTTGAATCAATTCTGG+AGG	-	chr7_2:7861178-7861197	Msa1047150:intron	30.0%
CGATGTGAAGAAAAATATAG+AGG	-	chr7_2:7861455-7861474	Msa1047150:intron	30.0%
CAACAAATGCTTATTCATCA+AGG	-	chr7_2:7861510-7861529	Msa1047150:CDS	30.0%
CATTGAATGTCAATGGATAA+TGG	+	chr7_2:7861950-7861969	None:intergenic	30.0%
CAAATATGCAGTTACAAAAG+TGG	-	chr7_2:7862058-7862077	Msa1047150:CDS	30.0%
AGGAAGGTTAAAAGACATAA+AGG	+	chr7_2:7862258-7862277	None:intergenic	30.0%
ACCCAAAAAATAATGAAGGA+AGG	+	chr7_2:7862274-7862293	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	7860316	7865653	7860316	ID=Msa1047150;Name=Msa1047150
chr7_2	mRNA	7860316	7865653	7860316	ID=Msa1047150-mRNA-1;Parent=Msa1047150;Name=Msa1047150-mRNA-1;_AED=0.06;_eAED=0.06;_QI=120\|1\|1\|1\|1\|1\|12\|291\|376
chr7_2	exon	7860316	7860723	7860316	ID=Msa1047150-mRNA-1:exon:2046;Parent=Msa1047150-mRNA-1
chr7_2	exon	7861047	7861125	7861047	ID=Msa1047150-mRNA-1:exon:2045;Parent=Msa1047150-mRNA-1
chr7_2	exon	7861470	7861573	7861470	ID=Msa1047150-mRNA-1:exon:2044;Parent=Msa1047150-mRNA-1
chr7_2	exon	7861651	7861698	7861651	ID=Msa1047150-mRNA-1:exon:2043;Parent=Msa1047150-mRNA-1
chr7_2	exon	7861786	7861851	7861786	ID=Msa1047150-mRNA-1:exon:2042;Parent=Msa1047150-mRNA-1
chr7_2	exon	7861941	7862084	7861941	ID=Msa1047150-mRNA-1:exon:2041;Parent=Msa1047150-mRNA-1
chr7_2	exon	7862600	7862777	7862600	ID=Msa1047150-mRNA-1:exon:2040;Parent=Msa1047150-mRNA-1
chr7_2	exon	7862976	7863020	7862976	ID=Msa1047150-mRNA-1:exon:2039;Parent=Msa1047150-mRNA-1
chr7_2	exon	7863109	7863182	7863109	ID=Msa1047150-mRNA-1:exon:2038;Parent=Msa1047150-mRNA-1
chr7_2	exon	7863483	7863530	7863483	ID=Msa1047150-mRNA-1:exon:2037;Parent=Msa1047150-mRNA-1
chr7_2	exon	7864358	7864519	7864358	ID=Msa1047150-mRNA-1:exon:2036;Parent=Msa1047150-mRNA-1
chr7_2	exon	7865468	7865653	7865468	ID=Msa1047150-mRNA-1:exon:2035;Parent=Msa1047150-mRNA-1
chr7_2	five_prime_UTR	7865534	7865653	7865534	ID=Msa1047150-mRNA-1:five_prime_utr;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7865468	7865533	7865468	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7864358	7864519	7864358	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7863483	7863530	7863483	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7863109	7863182	7863109	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7862976	7863020	7862976	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7862600	7862777	7862600	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7861941	7862084	7861941	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7861786	7861851	7861786	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7861651	7861698	7861651	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7861470	7861573	7861470	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7861047	7861125	7861047	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	CDS	7860607	7860723	7860607	ID=Msa1047150-mRNA-1:cds;Parent=Msa1047150-mRNA-1
chr7_2	three_prime_UTR	7860316	7860606	7860316	ID=Msa1047150-mRNA-1:three_prime_utr;Parent=Msa1047150-mRNA-1

Gene Sequence

>Msa1047150

ATGAAGATTAACGTGAAGACTCTTAAGGGAACTCACTTTGAGATCCAAGTGAACGCTCAAGATACGGTTGGCGATGTGAAGAAAAATATAGAGGGAGCACAGGGTGCTGCTGTTTACCCTGCTGCACAACAAATGCTTATTCATCAAGGAAAAGTGCTTAAGGATGAGACTACCTTGGAAGAAAATCAAGTTGCTGAGAATAGTTTTGTCGTGATCATGTTGTCTAAGAATAAGGTATCATCAAGTGGAGCCTCTGCTGCTTCAGCTGCCCCACCGGCAGCCCAACCTGCAAGTTCTTTGCCTCCCCCTTCGTCAACACCTCATCAGCCTCCTGCATCAACTGTCGGACAGGGAGAATCCAATCTTGCGCAAGGTCTTGTAACTCCTCCCACTACTGTGGTGCCTGACACATATGGCCAAGCTGAATCTAATCTCATTGCTGGAAGTACTTTAGAACCAACTATCCAACAAATTCTAGAAATGGGGGGAGGAAGTTGGGATCGGGACACTGTGATCCGAGCTCTTCGTGCTGCATATAATAATCCCGAAAGAGCTGTTGAATATCTATATTCTGGCATCCCCGAACAAGCTGAAGCTCCAGCAGTTGCTGCATCCACCAATGTTGGGCAGGAAGAAAACCCTTCAGCTCAGGCTCCAGCCCAACCAGCTGTGCCTGCTGTTGGGCCTAACACCAACCCACTGAACTTGTTCCCTCAGGGCATTCCCAATTTGGGTGCAAATGAAAATGCAGGTGACTTGGAATTTCTGCGAAACAGTCAACAGTTCCAAGCCTTGAGAACAATGGTGCAAGCAAACCCTCAAATCTTGCAGCCCATGCTTCAGGAACTAGGAAAACAAAATCCAAACCTAATGGAACTCATCCAAGAGCATCAAGCTGACTTCTTACGTCTTATAAATGAGCCTGGAGGCGAAGAGAACCTAGAGGGACAGTTGGGTGCTTTGGCTCCTCAGACCATTACTATCACCCCAGAAGAGAACGAGGCCATTCAACGGCTTGAGGATATGGGGTTCGACCGAGATCTTGTGTTGGAGGTGTTCTTTGCGTGCAATAAAAACGAGGATCTGGCAGCCAACTACCTATTGGATCACCAAAATGAGTTTGATGATTAG

Protein sequence

>Msa1047150

MKINVKTLKGTHFEIQVNAQDTVGDVKKNIEGAQGAAVYPAAQQMLIHQGKVLKDETTLEENQVAENSFVVIMLSKNKVSSSGASAASAAPPAAQPASSLPPPSSTPHQPPASTVGQGESNLAQGLVTPPTTVVPDTYGQAESNLIAGSTLEPTIQQILEMGGGSWDRDTVIRALRAAYNNPERAVEYLYSGIPEQAEAPAVAASTNVGQEENPSAQAPAQPAVPAVGPNTNPLNLFPQGIPNLGANENAGDLEFLRNSQQFQALRTMVQANPQILQPMLQELGKQNPNLMELIQEHQADFLRLINEPGGEENLEGQLGALAPQTITITPEENEAIQRLEDMGFDRDLVLEVFFACNKNEDLAANYLLDHQNEFDD*