AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1395530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395530 MtrunA17_Chr4g0064231 96.018 226 9 0 1 226 1 226 6.95e-154 426
Msa1395530 MtrunA17_Chr4g0033601 51.592 157 60 2 64 220 5 145 2.70e-44 145
Msa1395530 MtrunA17_Chr2g0329371 39.286 224 112 4 3 220 1 206 3.58e-38 132
Msa1395530 MtrunA17_Chr7g0256171 47.436 156 66 1 64 219 5 144 3.90e-35 122
Msa1395530 MtrunA17_Chr8g0392951 40.881 159 75 3 63 221 23 162 9.44e-28 103
Msa1395530 MtrunA17_Chr5g0434951 41.026 156 73 2 63 218 3 139 2.45e-27 101
Msa1395530 MtrunA17_Chr6g0482731 39.355 155 74 3 63 216 25 160 6.73e-27 101
Msa1395530 MtrunA17_Chr3g0111371 41.290 155 69 3 62 216 3 135 1.08e-26 100
Msa1395530 MtrunA17_Chr7g0245371 37.037 162 96 2 56 217 16 171 5.95e-26 99.4
Msa1395530 MtrunA17_Chr1g0188171 39.869 153 74 2 64 216 12 146 1.23e-25 97.8
Msa1395530 MtrunA17_Chr1g0188171 46.835 79 36 2 148 222 7 83 2.97e-11 59.7
Msa1395530 MtrunA17_Chr7g0254731 40.523 153 73 2 64 216 12 146 2.89e-25 96.7
Msa1395530 MtrunA17_Chr3g0093271 41.176 153 72 3 64 216 63 197 3.68e-25 97.8
Msa1395530 MtrunA17_Chr5g0440931 40.523 153 73 2 64 216 12 146 8.06e-25 95.5
Msa1395530 MtrunA17_Chr6g0460211 40.523 153 73 2 64 216 12 146 1.04e-24 95.1
Msa1395530 MtrunA17_Chr1g0169621 37.576 165 90 2 63 216 3 165 3.80e-24 94.4
Msa1395530 MtrunA17_Chr1g0161211 37.427 171 88 3 46 216 29 180 5.10e-24 94.7
Msa1395530 MtrunA17_Chr7g0245911 40.506 158 81 4 64 219 47 193 1.90e-23 93.2
Msa1395530 MtrunA17_Chr1g0166281 40.127 157 76 3 64 220 12 150 5.83e-23 90.9
Msa1395530 MtrunA17_Chr5g0402331 37.368 190 88 7 38 219 19 185 4.12e-22 89.7
Msa1395530 MtrunA17_Chr1g0169581 38.961 154 78 4 63 216 3 140 4.27e-22 88.2
Msa1395530 MtrunA17_Chr5g0402361 37.368 190 88 7 38 219 191 357 1.16e-20 89.0
Msa1395530 MtrunA17_Chr5g0402361 36.364 187 96 6 37 219 17 184 8.10e-20 86.7
Msa1395530 MtrunA17_Chr8g0391691 39.103 156 77 3 64 219 36 173 1.76e-20 85.5
Msa1395530 MtrunA17_Chr4g0044961 37.662 154 76 4 63 216 56 189 3.31e-20 84.7
Msa1395530 MtrunA17_Chr8g0366401 31.210 157 93 3 64 220 17 158 4.70e-20 83.6
Msa1395530 MtrunA17_Chr7g0225921 36.943 157 73 5 64 216 6 140 7.00e-20 82.4
Msa1395530 MtrunA17_Chr8g0351681 38.608 158 83 4 64 219 49 194 2.12e-19 82.8
Msa1395530 MtrunA17_Chr4g0057641 34.416 154 82 3 64 217 6 140 5.68e-19 80.1
Msa1395530 MtrunA17_Chr6g0459231 36.000 150 77 3 67 216 11 141 6.63e-19 79.7
Msa1395530 MtrunA17_Chr3g0125311 35.897 156 88 3 64 216 13 159 1.99e-18 79.3
Msa1395530 MtrunA17_Chr4g0057651 35.294 153 80 3 64 216 6 139 4.33e-18 77.8
Msa1395530 MtrunA17_Chr0c02g0489761 32.903 155 84 2 65 219 384 518 2.82e-17 80.1
Msa1395530 MtrunA17_Chr1g0185001 34.641 153 81 2 64 216 12 145 3.08e-17 75.5
Msa1395530 MtrunA17_Chr5g0441691 33.548 155 83 3 65 219 374 508 3.67e-17 79.7
Msa1395530 MtrunA17_Chr3g0116151 33.117 154 80 5 66 217 8 140 3.68e-17 75.1
Msa1395530 MtrunA17_Chr4g0057661 33.987 153 82 3 64 216 6 139 3.76e-17 75.1
Msa1395530 MtrunA17_Chr3g0116141 33.117 154 80 5 66 217 7 139 4.07e-17 75.1
Msa1395530 MtrunA17_Chr1g0160151 33.526 173 98 4 47 216 2 160 1.85e-16 73.9
Msa1395530 MtrunA17_Chr4g0062221 31.056 161 95 3 64 224 11 155 1.93e-16 73.9
Msa1395530 MtrunA17_Chr8g0373691 29.480 173 102 4 45 216 34 187 5.92e-16 73.2
Msa1395530 MtrunA17_Chr4g0074811 34.194 155 82 3 65 219 397 531 8.01e-16 75.9
Msa1395530 MtrunA17_Chr5g0444171 31.613 155 86 3 65 219 398 532 1.45e-14 72.0
Msa1395530 MtrunA17_Chr1g0157131 32.903 155 84 3 65 219 380 514 2.51e-14 71.6
Msa1395530 MtrunA17_Chr8g0387671 32.258 155 85 2 65 219 372 506 2.54e-14 71.2
Msa1395530 MtrunA17_Chr8g0385341 31.613 155 85 3 65 219 336 469 3.27e-14 71.2
Msa1395530 MtrunA17_Chr3g0131181 30.968 155 86 3 65 219 335 468 3.52e-14 70.9
Msa1395530 MtrunA17_Chr7g0256701 35.484 155 83 5 64 216 73 212 3.78e-14 68.9
Msa1395530 MtrunA17_Chr5g0397101 31.447 159 89 2 65 223 372 510 7.75e-14 70.1
Msa1395530 MtrunA17_Chr7g0267751 33.962 159 84 4 65 223 441 578 1.53e-13 69.3
Msa1395530 MtrunA17_Chr4g0059951 31.646 158 87 3 63 219 377 514 1.62e-13 68.9
Msa1395530 MtrunA17_Chr7g0245231 29.193 161 96 3 62 222 28 170 1.89e-13 66.2
Msa1395530 MtrunA17_Chr1g0200601 31.613 155 85 3 65 219 336 469 1.96e-13 68.9
Msa1395530 MtrunA17_Chr5g0396261 40.517 116 53 5 100 215 13 112 1.99e-13 64.7
Msa1395530 MtrunA17_Chr5g0408251 44.595 74 41 0 145 218 124 197 2.30e-13 66.6
Msa1395530 MtrunA17_Chr7g0217621 44.595 74 41 0 145 218 126 199 4.58e-13 65.9
Msa1395530 MtrunA17_Chr7g0242561 28.662 157 93 2 63 219 360 497 4.84e-13 67.8
Msa1395530 MtrunA17_Chr1g0176751 32.075 159 87 3 65 223 430 567 4.93e-13 67.8
Msa1395530 MtrunA17_Chr3g0098951 30.189 159 90 3 65 219 389 530 1.80e-12 65.9
Msa1395530 MtrunA17_Chr5g0441781 41.667 72 39 1 151 222 9 77 1.91e-12 61.2
Msa1395530 MtrunA17_Chr1g0170281 32.258 155 84 3 65 219 424 557 2.17e-12 65.9
Msa1395530 MtrunA17_Chr4g0071941 49.231 65 33 0 154 218 83 147 4.28e-12 62.0
Msa1395530 MtrunA17_Chr6g0450921 29.032 155 92 3 62 216 27 163 5.00e-12 62.4
Msa1395530 MtrunA17_Chr4g0074791 32.903 155 74 4 65 219 401 525 8.47e-12 63.9
Msa1395530 MtrunA17_Chr8g0368681 30.818 159 94 4 63 221 11 153 1.16e-11 60.8
Msa1395530 MtrunA17_Chr5g0448721 31.410 156 84 4 65 219 402 535 1.56e-11 63.2
Msa1395530 MtrunA17_Chr4g0033371 27.778 162 97 2 65 226 378 519 5.84e-11 61.6
Msa1395530 MtrunA17_Chr7g0274101 32.680 153 85 2 64 216 12 146 7.95e-11 58.5
Msa1395530 MtrunA17_Chr3g0121191 31.288 163 72 6 64 220 93 221 9.15e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395530 AT1G05990.1 49.045 157 65 1 64 220 5 146 1.15e-40 136
Msa1395530 AT2G43290.1 47.134 157 72 1 64 220 65 210 1.54e-40 138
Msa1395530 AT3G59440.1 38.991 218 104 4 2 219 1 189 8.85e-40 135
Msa1395530 AT3G07490.1 47.170 159 68 1 62 220 3 145 5.75e-38 129
Msa1395530 AT4G03290.1 48.077 156 68 1 64 219 5 147 2.07e-36 125
Msa1395530 AT4G12860.1 45.912 159 70 1 62 220 3 145 1.05e-34 121
Msa1395530 AT5G37770.1 42.767 159 77 3 63 221 16 160 6.03e-28 104
Msa1395530 AT5G37770.2 42.767 159 77 3 63 221 16 160 6.03e-28 104
Msa1395530 AT4G20780.1 36.898 187 106 3 38 217 3 184 1.36e-27 104
Msa1395530 AT1G18210.2 38.710 155 73 2 62 216 21 153 3.57e-26 100
Msa1395530 AT1G18210.1 38.710 155 73 2 62 216 21 153 3.57e-26 100
Msa1395530 AT1G66410.1 40.523 153 73 2 64 216 12 146 4.94e-25 96.7
Msa1395530 AT5G37780.1 40.523 153 73 2 64 216 12 146 4.94e-25 96.7
Msa1395530 AT5G44460.1 39.394 165 88 2 56 217 20 175 5.01e-25 97.4
Msa1395530 AT5G17470.1 40.373 161 76 4 65 223 3 145 5.24e-25 96.3
Msa1395530 AT4G14640.1 39.355 155 76 2 64 218 13 149 9.88e-25 95.9
Msa1395530 AT3G22930.1 40.625 160 71 4 64 220 35 173 1.21e-24 96.3
Msa1395530 AT5G21274.1 40.523 153 73 2 64 216 12 146 1.22e-24 95.5
Msa1395530 AT3G43810.1 40.523 153 73 2 64 216 12 146 1.44e-24 95.1
Msa1395530 AT3G43810.2 41.667 156 72 3 64 218 12 149 1.75e-24 96.7
Msa1395530 AT2G27030.3 38.788 165 81 3 64 226 12 158 2.98e-24 95.5
Msa1395530 AT3G56800.1 39.869 153 74 2 64 216 12 146 6.52e-24 93.6
Msa1395530 AT2G41110.1 39.869 153 74 2 64 216 12 146 6.52e-24 93.6
Msa1395530 AT2G27030.1 39.869 153 74 2 64 216 12 146 6.52e-24 93.6
Msa1395530 AT1G66400.1 40.000 160 79 4 63 221 14 157 2.11e-23 92.4
Msa1395530 AT1G24620.1 36.170 188 99 4 38 222 8 177 2.48e-23 93.2
Msa1395530 AT1G73630.1 34.078 179 89 3 40 218 3 152 5.05e-23 91.7
Msa1395530 AT2G41110.2 36.970 165 74 3 64 216 12 158 3.39e-21 86.7
Msa1395530 AT2G36180.1 39.744 156 75 4 65 218 1 139 3.61e-21 86.3
Msa1395530 AT5G37780.2 37.500 168 72 4 64 216 12 161 4.60e-21 86.7
Msa1395530 AT2G41100.7 36.000 150 93 2 64 212 66 213 1.96e-19 85.1
Msa1395530 AT2G41100.6 36.000 150 93 2 64 212 66 213 1.96e-19 85.1
Msa1395530 AT2G41100.3 36.000 150 93 2 64 212 66 213 1.96e-19 85.1
Msa1395530 AT4G14640.2 37.589 141 70 2 78 218 9 131 2.27e-19 81.3
Msa1395530 AT5G37780.3 34.637 179 73 3 64 216 12 172 2.41e-19 82.4
Msa1395530 AT2G41100.4 36.000 150 93 2 64 212 101 248 3.85e-19 84.7
Msa1395530 AT2G41100.4 35.185 162 94 4 64 220 12 167 1.20e-18 83.2
Msa1395530 AT2G41100.1 36.000 150 93 2 64 212 101 248 3.85e-19 84.7
Msa1395530 AT2G41100.1 35.185 162 94 4 64 220 12 167 1.20e-18 83.2
Msa1395530 AT5G37780.4 38.849 139 67 3 78 216 13 133 2.36e-18 78.6
Msa1395530 AT1G66410.2 38.129 139 68 2 78 216 36 156 4.01e-18 78.6
Msa1395530 AT2G15680.1 36.709 158 74 5 64 218 50 184 4.64e-18 79.3
Msa1395530 AT2G41090.1 32.500 160 83 4 64 220 12 149 6.02e-18 79.0
Msa1395530 AT1G32250.1 28.662 157 100 2 64 220 16 160 1.63e-17 77.4
Msa1395530 AT4G37010.2 32.919 161 88 5 58 217 25 166 3.40e-17 76.6
Msa1395530 AT3G10190.1 32.353 170 96 4 48 216 54 205 3.72e-17 77.4
Msa1395530 AT3G50360.1 33.121 157 87 3 62 218 25 163 3.81e-17 76.3
Msa1395530 AT4G37010.1 32.919 161 88 5 58 217 21 162 4.36e-17 76.3
Msa1395530 AT5G12180.1 33.548 155 83 3 65 219 379 513 5.10e-17 79.7
Msa1395530 AT1G66410.4 40.336 119 53 3 98 216 10 110 1.28e-16 73.6
Msa1395530 AT1G66410.3 40.336 119 53 3 98 216 10 110 1.28e-16 73.6
Msa1395530 AT5G19360.1 33.548 155 83 3 65 219 374 508 1.94e-16 78.2
Msa1395530 AT3G43810.4 40.336 119 53 3 98 216 10 110 3.04e-16 72.4
Msa1395530 AT3G43810.3 40.336 119 53 3 98 216 10 110 3.04e-16 72.4
Msa1395530 AT3G20410.2 32.911 158 86 3 65 222 397 534 4.02e-15 74.3
Msa1395530 AT3G20410.1 32.911 158 86 3 65 222 397 534 4.02e-15 74.3
Msa1395530 AT4G04695.1 32.335 167 90 3 58 221 327 473 4.23e-15 73.9
Msa1395530 AT4G23650.1 32.704 159 87 3 65 223 384 522 4.50e-15 73.9
Msa1395530 AT3G50770.1 31.410 156 92 3 62 216 62 203 6.51e-15 71.2
Msa1395530 AT1G18530.1 31.515 165 89 4 64 224 7 151 8.99e-15 69.7
Msa1395530 AT1G50700.1 32.278 158 87 3 65 222 379 516 1.02e-14 73.2
Msa1395530 AT4G04740.4 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT4G04740.6 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT4G04740.5 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT4G04740.3 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT4G04740.1 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT4G04740.7 31.613 155 86 2 65 219 374 508 1.47e-14 72.4
Msa1395530 AT3G51920.1 30.519 154 89 2 63 216 11 146 1.50e-14 68.9
Msa1395530 AT2G31500.1 30.323 155 88 3 67 219 374 510 2.06e-14 72.0
Msa1395530 AT1G76640.1 31.818 154 89 2 64 217 22 159 2.69e-14 68.6
Msa1395530 AT5G23580.1 32.680 153 82 3 65 217 328 459 3.17e-14 71.6
Msa1395530 AT5G42380.1 34.416 154 84 4 64 217 49 185 3.22e-14 68.9
Msa1395530 AT1G21550.1 35.714 154 87 3 64 216 10 152 3.55e-14 68.2
Msa1395530 AT3G25600.1 29.560 159 96 2 63 221 11 153 4.23e-14 68.2
Msa1395530 AT3G10660.2 33.974 156 82 4 65 220 492 626 8.16e-14 70.5
Msa1395530 AT3G10660.1 33.974 156 82 4 65 220 492 626 8.16e-14 70.5
Msa1395530 AT4G04720.2 32.278 158 87 3 65 222 385 522 1.28e-13 69.7
Msa1395530 AT4G04720.1 32.278 158 87 3 65 222 385 522 1.28e-13 69.7
Msa1395530 AT3G03400.1 29.252 147 84 3 65 209 6 134 1.76e-13 65.9
Msa1395530 AT3G03000.1 31.210 157 91 3 64 220 20 159 2.35e-13 66.2
Msa1395530 AT1G61950.1 34.194 155 80 3 65 218 405 538 2.59e-13 68.9
Msa1395530 AT4G09570.1 31.613 155 85 3 65 219 331 464 3.01e-13 68.6
Msa1395530 AT4G21940.2 31.613 155 86 2 65 219 407 541 3.03e-13 68.6
Msa1395530 AT4G21940.1 31.613 155 86 2 65 219 407 541 3.05e-13 68.6
Msa1395530 AT1G35670.1 30.968 155 86 3 65 219 332 465 3.42e-13 68.6
Msa1395530 AT4G04700.1 32.317 164 88 3 58 218 327 470 6.80e-13 67.8
Msa1395530 AT1G76040.1 32.903 155 84 3 65 219 179 313 7.53e-13 67.0
Msa1395530 AT1G76040.4 32.903 155 84 3 65 219 179 313 7.53e-13 67.0
Msa1395530 AT1G76650.1 31.169 154 87 2 64 217 43 177 7.94e-13 65.1
Msa1395530 AT1G76650.3 31.169 154 87 2 64 217 43 177 7.94e-13 65.1
Msa1395530 AT5G04870.1 33.333 156 83 4 65 220 456 590 1.41e-12 66.6
Msa1395530 AT1G76040.2 32.903 155 84 3 65 219 417 551 1.58e-12 66.6
Msa1395530 AT3G22930.2 37.391 115 50 3 64 175 35 130 3.33e-12 62.0
Msa1395530 AT3G29000.1 44.737 76 39 1 147 219 117 192 3.76e-12 63.5
Msa1395530 AT2G41100.2 35.088 114 71 2 100 212 12 123 3.86e-12 63.5
Msa1395530 AT1G50700.2 29.762 168 104 4 65 222 379 542 4.65e-12 65.1
Msa1395530 AT4G38230.2 30.968 155 86 3 65 219 360 493 5.28e-12 65.1
Msa1395530 AT3G51850.1 28.025 157 94 3 63 219 358 495 5.44e-12 65.1
Msa1395530 AT4G38230.4 30.968 155 86 3 65 219 330 463 6.10e-12 64.7
Msa1395530 AT4G38230.3 30.968 155 86 3 65 219 330 463 6.10e-12 64.7
Msa1395530 AT4G35310.2 29.299 157 90 3 65 221 403 538 6.32e-12 64.7
Msa1395530 AT4G35310.1 29.299 157 90 3 65 221 403 538 6.32e-12 64.7
Msa1395530 AT2G17290.1 29.936 157 89 3 65 221 391 526 7.16e-12 64.7
Msa1395530 AT2G17290.2 29.936 157 89 3 65 221 391 526 7.16e-12 64.7
Msa1395530 AT5G39670.1 44.737 76 38 1 143 218 130 201 7.60e-12 62.8
Msa1395530 AT4G38230.1 30.968 155 86 3 65 219 186 319 7.80e-12 64.3
Msa1395530 AT4G14640.3 36.036 111 55 1 64 174 13 107 1.20e-11 60.5
Msa1395530 AT4G36070.1 27.439 164 93 4 63 222 377 518 1.42e-11 63.9
Msa1395530 AT4G36070.2 27.439 164 93 4 63 222 377 518 1.61e-11 63.5

Find 50 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATTAGAGGCTAATTTAA+AGG 0.262554 tig0021948:+45685 Msa1395530:CDS
TTTGTTGACTAATGAGTTTA+TGG 0.315498 tig0021948:+45607 Msa1395530:CDS
ACAAATATTACTATTGTTAG+TGG 0.337977 tig0021948:-45351 Msa1395530:intergenic
TTGGAAAGGGATGCTAATTA+TGG 0.341837 tig0021948:+45401 Msa1395530:CDS
GTTGGCAAGTTTGATTCTAA+TGG 0.351384 tig0021948:+45554 Msa1395530:CDS
GATGATTAGGAAAGTTGATA+TGG 0.351439 tig0021948:+45823 Msa1395530:CDS
AGTAGGTGGTGTTGGTAGTA+AGG 0.352457 tig0021948:+45649 Msa1395530:CDS
AAGAGTGTAAAGAGATGATT+AGG 0.368467 tig0021948:+45810 Msa1395530:CDS
AAGAGTTGAAGGAATCATTA+AGG 0.371802 tig0021948:+45489 Msa1395530:CDS
AGCTTTGGTTCTTTGTTCGT+TGG 0.384179 tig0021948:+45763 Msa1395530:CDS
AAAGAGATTGATGATATTGT+TGG 0.394302 tig0021948:+45536 Msa1395530:CDS
AGATGAATTGGAAGAATTAG+AGG 0.396498 tig0021948:+45673 Msa1395530:CDS
TTCAGTGGAGGAGCTAGCTT+TGG 0.409228 tig0021948:+45748 Msa1395530:CDS
TTCAAGAGAATGATGAAGAA+TGG 0.413616 tig0021948:+45875 Msa1395530:CDS
TCAAGAGAATGATGAAGAAT+GGG 0.414403 tig0021948:+45876 Msa1395530:CDS
CATAACAAAGCAAGAGTTGA+AGG 0.422923 tig0021948:+45478 Msa1395530:CDS
TGGTAGTAAGGAAGATGAAT+TGG 0.428008 tig0021948:+45661 Msa1395530:CDS
ATTAGCATCCCTTTCCAACT+TGG 0.428605 tig0021948:-45396 Msa1395530:intergenic
TGTAAAAGATTATATTTATG+AGG 0.433393 tig0021948:-45286 Msa1395530:intergenic
GCTTTGGTTCTTTGTTCGTT+GGG 0.435248 tig0021948:+45764 Msa1395530:CDS
AAGGAACATAAGGATCTTCA+TGG 0.444129 tig0021948:+45508 Msa1395530:CDS
AGGAATCATTAAGGAACATA+AGG 0.460423 tig0021948:+45498 Msa1395530:CDS
TTGTTGACTAATGAGTTTAT+GGG 0.462773 tig0021948:+45608 Msa1395530:CDS
GACTAATGAGTTTATGGGAG+GGG 0.477111 tig0021948:+45613 Msa1395530:CDS
CAAATATTACTATTGTTAGT+GGG 0.481063 tig0021948:-45350 Msa1395530:intergenic
GTGTTTGACAAAGATAATGA+TGG 0.506462 tig0021948:+45719 Msa1395530:CDS
ACTAATGAGTTTATGGGAGG+GGG 0.514679 tig0021948:+45614 Msa1395530:CDS
ACTTTCGACAAGAATGGTGA+TGG 0.517448 tig0021948:+45452 Msa1395530:CDS
GGTGAGAAAGTAGGTGGTGT+TGG 0.518841 tig0021948:+45641 Msa1395530:CDS
GTTCGTTGGGATTAAGTGAA+GGG 0.520871 tig0021948:+45777 Msa1395530:CDS
ATTAGGAAAGTTGATATGGA+TGG 0.528875 tig0021948:+45827 Msa1395530:CDS
TACAACGACCAAGTTGGAAA+GGG 0.542486 tig0021948:+45388 Msa1395530:CDS
CAAGAGAATGATGAAGAATG+GGG 0.544274 tig0021948:+45877 Msa1395530:CDS
TTGACTAATGAGTTTATGGG+AGG 0.548250 tig0021948:+45611 Msa1395530:CDS
TGTTCGTTGGGATTAAGTGA+AGG 0.552638 tig0021948:+45776 Msa1395530:CDS
TAATGATGGGTTAATTTCAG+TGG 0.553320 tig0021948:+45733 Msa1395530:CDS
CGTGGCTACAACGACCAAGT+TGG 0.554203 tig0021948:+45382 Msa1395530:CDS
ATTAGAGGCTAATTTAAAGG+AGG 0.574173 tig0021948:+45688 Msa1395530:CDS
AAAGTTGATATGGATGGAGA+TGG 0.588783 tig0021948:+45833 Msa1395530:CDS
CTACAACGACCAAGTTGGAA+AGG 0.597253 tig0021948:+45387 Msa1395530:CDS
TGATGGGTTAATTTCAGTGG+AGG 0.600537 tig0021948:+45736 Msa1395530:CDS
AAGGGATGCTAATTATGGAA+AGG 0.607934 tig0021948:+45406 Msa1395530:CDS
CTTGGTCGTTGTAGCCACGT+TGG 0.608119 tig0021948:-45378 Msa1395530:intergenic
AGAGGCTGAAATGGATTATG+TGG 0.612244 tig0021948:-45915 Msa1395530:intergenic
TGACTAATGAGTTTATGGGA+GGG 0.620317 tig0021948:+45612 Msa1395530:CDS
GGTGAAGGTGAGAAAGTAGG+TGG 0.621976 tig0021948:+45635 Msa1395530:CDS
TGTTTGACAAAGATAATGAT+GGG 0.622546 tig0021948:+45720 Msa1395530:CDS
CAAAAGTGTAGCAAGAACCA+TGG 0.637789 tig0021948:-45240 Msa1395530:intergenic
AAGAGAATGATGAAGAATGG+GGG 0.685392 tig0021948:+45878 Msa1395530:CDS
TAATATTTGTAAAACCAACG+TGG 0.772101 tig0021948:+45364 Msa1395530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAAAAGATTATATTTATG+AGG - tig0021948:45289-45308 Msa1395530:intergenic 15.0%
!! CAAATATTACTATTGTTAGT+GGG - tig0021948:45353-45372 Msa1395530:intergenic 20.0%
!! ACAAATATTACTATTGTTAG+TGG - tig0021948:45354-45373 Msa1395530:intergenic 20.0%
! TAATATTTGTAAAACCAACG+TGG + tig0021948:45364-45383 Msa1395530:CDS 25.0%
!! AAAGAGATTGATGATATTGT+TGG + tig0021948:45536-45555 Msa1395530:CDS 25.0%
!! TTTGTTGACTAATGAGTTTA+TGG + tig0021948:45607-45626 Msa1395530:CDS 25.0%
!! TTGTTGACTAATGAGTTTAT+GGG + tig0021948:45608-45627 Msa1395530:CDS 25.0%
! AGAATTAGAGGCTAATTTAA+AGG + tig0021948:45685-45704 Msa1395530:CDS 25.0%
! TGTTTGACAAAGATAATGAT+GGG + tig0021948:45720-45739 Msa1395530:CDS 25.0%
!! AAGAAAAGCACGAATTTTGT+TGG - tig0021948:45318-45337 Msa1395530:intergenic 30.0%
AAGAGTTGAAGGAATCATTA+AGG + tig0021948:45489-45508 Msa1395530:CDS 30.0%
AGGAATCATTAAGGAACATA+AGG + tig0021948:45498-45517 Msa1395530:CDS 30.0%
AGATGAATTGGAAGAATTAG+AGG + tig0021948:45673-45692 Msa1395530:CDS 30.0%
ATTAGAGGCTAATTTAAAGG+AGG + tig0021948:45688-45707 Msa1395530:CDS 30.0%
GTGTTTGACAAAGATAATGA+TGG + tig0021948:45719-45738 Msa1395530:CDS 30.0%
!! TAATGATGGGTTAATTTCAG+TGG + tig0021948:45733-45752 Msa1395530:CDS 30.0%
! AAGAGTGTAAAGAGATGATT+AGG + tig0021948:45810-45829 Msa1395530:CDS 30.0%
!! GATGATTAGGAAAGTTGATA+TGG + tig0021948:45823-45842 Msa1395530:CDS 30.0%
!! ATTAGGAAAGTTGATATGGA+TGG + tig0021948:45827-45846 Msa1395530:CDS 30.0%
TTCAAGAGAATGATGAAGAA+TGG + tig0021948:45875-45894 Msa1395530:CDS 30.0%
TCAAGAGAATGATGAAGAAT+GGG + tig0021948:45876-45895 Msa1395530:CDS 30.0%
! AAAGCACGAATTTTGTTGGT+AGG - tig0021948:45314-45333 Msa1395530:intergenic 35.0%
TTGGAAAGGGATGCTAATTA+TGG + tig0021948:45401-45420 Msa1395530:CDS 35.0%
AAGGGATGCTAATTATGGAA+AGG + tig0021948:45406-45425 Msa1395530:CDS 35.0%
! TTTTCGACTTTCGACAAGAA+TGG + tig0021948:45446-45465 Msa1395530:CDS 35.0%
CATAACAAAGCAAGAGTTGA+AGG + tig0021948:45478-45497 Msa1395530:CDS 35.0%
AAGGAACATAAGGATCTTCA+TGG + tig0021948:45508-45527 Msa1395530:CDS 35.0%
GTTGGCAAGTTTGATTCTAA+TGG + tig0021948:45554-45573 Msa1395530:CDS 35.0%
TTGACTAATGAGTTTATGGG+AGG + tig0021948:45611-45630 Msa1395530:CDS 35.0%
TGACTAATGAGTTTATGGGA+GGG + tig0021948:45612-45631 Msa1395530:CDS 35.0%
TGGTAGTAAGGAAGATGAAT+TGG + tig0021948:45661-45680 Msa1395530:CDS 35.0%
!! AAAGTTGATATGGATGGAGA+TGG + tig0021948:45833-45852 Msa1395530:CDS 35.0%
CAAGAGAATGATGAAGAATG+GGG + tig0021948:45877-45896 Msa1395530:CDS 35.0%
AAGAGAATGATGAAGAATGG+GGG + tig0021948:45878-45897 Msa1395530:CDS 35.0%
! TCTTGCTACACTTTTGCTAG+TGG + tig0021948:45247-45266 Msa1395530:CDS 40.0%
! CTAGTGGTTCTTTTCATTGC+AGG + tig0021948:45263-45282 Msa1395530:CDS 40.0%
! TACAACGACCAAGTTGGAAA+GGG + tig0021948:45388-45407 Msa1395530:CDS 40.0%
ATTAGCATCCCTTTCCAACT+TGG - tig0021948:45399-45418 Msa1395530:intergenic 40.0%
ACTTTCGACAAGAATGGTGA+TGG + tig0021948:45452-45471 Msa1395530:CDS 40.0%
GACTAATGAGTTTATGGGAG+GGG + tig0021948:45613-45632 Msa1395530:CDS 40.0%
ACTAATGAGTTTATGGGAGG+GGG + tig0021948:45614-45633 Msa1395530:CDS 40.0%
!! TGATGGGTTAATTTCAGTGG+AGG + tig0021948:45736-45755 Msa1395530:CDS 40.0%
!! AGCTTTGGTTCTTTGTTCGT+TGG + tig0021948:45763-45782 Msa1395530:CDS 40.0%
!! GCTTTGGTTCTTTGTTCGTT+GGG + tig0021948:45764-45783 Msa1395530:CDS 40.0%
TGTTCGTTGGGATTAAGTGA+AGG + tig0021948:45776-45795 Msa1395530:CDS 40.0%
GTTCGTTGGGATTAAGTGAA+GGG + tig0021948:45777-45796 Msa1395530:CDS 40.0%
! CTACAACGACCAAGTTGGAA+AGG + tig0021948:45387-45406 Msa1395530:CDS 45.0%
!! AGTAGGTGGTGTTGGTAGTA+AGG + tig0021948:45649-45668 Msa1395530:CDS 45.0%
GGTGAAGGTGAGAAAGTAGG+TGG + tig0021948:45635-45654 Msa1395530:CDS 50.0%
GGTGAGAAAGTAGGTGGTGT+TGG + tig0021948:45641-45660 Msa1395530:CDS 50.0%
! TTCAGTGGAGGAGCTAGCTT+TGG + tig0021948:45748-45767 Msa1395530:CDS 50.0%
CTTGGTCGTTGTAGCCACGT+TGG - tig0021948:45381-45400 Msa1395530:intergenic 55.0%
CGTGGCTACAACGACCAAGT+TGG + tig0021948:45382-45401 Msa1395530:CDS 55.0%
GAGTTTATGGGAGGGGGTGA+AGG + tig0021948:45620-45639 Msa1395530:CDS 55.0%
GGGGGTGAAGGTGAGAAAGT+AGG + tig0021948:45632-45651 Msa1395530:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0021948 gene 45242 45922 45242 ID=Msa1395530;Name=Msa1395530
tig0021948 mRNA 45242 45922 45242 ID=Msa1395530-mRNA-1;Parent=Msa1395530;Name=Msa1395530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|226
tig0021948 exon 45242 45922 45242 ID=Msa1395530-mRNA-1:exon:14917;Parent=Msa1395530-mRNA-1
tig0021948 CDS 45242 45922 45242 ID=Msa1395530-mRNA-1:cds;Parent=Msa1395530-mRNA-1
Gene Sequence

>Msa1395530

ATGGTTCTTGCTACACTTTTGCTAGTGGTTCTTTTCATTGCAGGCCTCATAAATATAATCTTTTACATACCTACCAACAAAATTCGTGCTTTTCTTCAAATCATTTTTCCCACTAACAATAGTAATATTTGTAAAACCAACGTGGCTACAACGACCAAGTTGGAAAGGGATGCTAATTATGGAAAGGAAGAACTCAAGAAAGTGTTTTCGACTTTCGACAAGAATGGTGATGGATTCATAACAAAGCAAGAGTTGAAGGAATCATTAAGGAACATAAGGATCTTCATGGACGAGAAAGAGATTGATGATATTGTTGGCAAGTTTGATTCTAATGGAGATGAGTTGATTGACTTTGATGAGTTTTGTTTGTTGACTAATGAGTTTATGGGAGGGGGTGAAGGTGAGAAAGTAGGTGGTGTTGGTAGTAAGGAAGATGAATTGGAAGAATTAGAGGCTAATTTAAAGGAGGCTTTTGATGTGTTTGACAAAGATAATGATGGGTTAATTTCAGTGGAGGAGCTAGCTTTGGTTCTTTGTTCGTTGGGATTAAGTGAAGGGAATAAGATTGAAGAGTGTAAAGAGATGATTAGGAAAGTTGATATGGATGGAGATGGTAATGTAAATTTTAATGAGTTCAAGAGAATGATGAAGAATGGGGGAGCAAAACTCTTTGCCACATAA
Protein sequence

>Msa1395530

MVLATLLLVVLFIAGLINIIFYIPTNKIRAFLQIIFPTNNSNICKTNVATTTKLERDANYGKEELKKVFSTFDKNGDGFITKQELKESLRNIRIFMDEKEIDDIVGKFDSNGDELIDFDEFCLLTNEFMGGGEGEKVGGVGSKEDELEELEANLKEAFDVFDKDNDGLISVEELALVLCSLGLSEGNKIEECKEMIRKVDMDGDGNVNFNEFKRMMKNGGAKLFAT*