Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395530 | XP_003609394.1 | 96.018 | 226 | 9 | 0 | 1 | 226 | 1 | 226 | 7.56e-150 | 426 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395530 | sp|Q9LNE7|CML7_ARATH | 49.045 | 157 | 65 | 1 | 64 | 220 | 5 | 146 | 1.13e-39 | 136 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000550 | Msa1395530 | -0.856906 | 2.343775e-62 | -8.615850e-47 |
| Msa0022310 | Msa1395530 | 0.814381 | 1.585629e-51 | -8.615850e-47 |
| Msa0029540 | Msa1395530 | -0.845097 | 5.022103e-59 | -8.615850e-47 |
| Msa0031360 | Msa1395530 | 0.806881 | 6.631331e-50 | -8.615850e-47 |
| Msa0032370 | Msa1395530 | 0.821288 | 4.371817e-53 | -8.615850e-47 |
| Msa0039330 | Msa1395530 | 0.802136 | 6.474973e-49 | -8.615850e-47 |
| Msa0039420 | Msa1395530 | -0.811843 | 5.716399e-51 | -8.615850e-47 |
| Msa0048000 | Msa1395530 | 0.820928 | 5.292738e-53 | -8.615850e-47 |
| Msa0050730 | Msa1395530 | 0.814801 | 1.280314e-51 | -8.615850e-47 |
| Msa0050760 | Msa1395530 | 0.813582 | 2.379298e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395530 | MtrunA17_Chr4g0064231 | 96.018 | 226 | 9 | 0 | 1 | 226 | 1 | 226 | 6.95e-154 | 426 |
| Msa1395530 | MtrunA17_Chr4g0033601 | 51.592 | 157 | 60 | 2 | 64 | 220 | 5 | 145 | 2.70e-44 | 145 |
| Msa1395530 | MtrunA17_Chr2g0329371 | 39.286 | 224 | 112 | 4 | 3 | 220 | 1 | 206 | 3.58e-38 | 132 |
| Msa1395530 | MtrunA17_Chr7g0256171 | 47.436 | 156 | 66 | 1 | 64 | 219 | 5 | 144 | 3.90e-35 | 122 |
| Msa1395530 | MtrunA17_Chr8g0392951 | 40.881 | 159 | 75 | 3 | 63 | 221 | 23 | 162 | 9.44e-28 | 103 |
| Msa1395530 | MtrunA17_Chr5g0434951 | 41.026 | 156 | 73 | 2 | 63 | 218 | 3 | 139 | 2.45e-27 | 101 |
| Msa1395530 | MtrunA17_Chr6g0482731 | 39.355 | 155 | 74 | 3 | 63 | 216 | 25 | 160 | 6.73e-27 | 101 |
| Msa1395530 | MtrunA17_Chr3g0111371 | 41.290 | 155 | 69 | 3 | 62 | 216 | 3 | 135 | 1.08e-26 | 100 |
| Msa1395530 | MtrunA17_Chr7g0245371 | 37.037 | 162 | 96 | 2 | 56 | 217 | 16 | 171 | 5.95e-26 | 99.4 |
| Msa1395530 | MtrunA17_Chr1g0188171 | 39.869 | 153 | 74 | 2 | 64 | 216 | 12 | 146 | 1.23e-25 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1395530 | AT1G05990.1 | 49.045 | 157 | 65 | 1 | 64 | 220 | 5 | 146 | 1.15e-40 | 136 |
| Msa1395530 | AT2G43290.1 | 47.134 | 157 | 72 | 1 | 64 | 220 | 65 | 210 | 1.54e-40 | 138 |
| Msa1395530 | AT3G59440.1 | 38.991 | 218 | 104 | 4 | 2 | 219 | 1 | 189 | 8.85e-40 | 135 |
| Msa1395530 | AT3G07490.1 | 47.170 | 159 | 68 | 1 | 62 | 220 | 3 | 145 | 5.75e-38 | 129 |
| Msa1395530 | AT4G03290.1 | 48.077 | 156 | 68 | 1 | 64 | 219 | 5 | 147 | 2.07e-36 | 125 |
| Msa1395530 | AT4G12860.1 | 45.912 | 159 | 70 | 1 | 62 | 220 | 3 | 145 | 1.05e-34 | 121 |
| Msa1395530 | AT5G37770.1 | 42.767 | 159 | 77 | 3 | 63 | 221 | 16 | 160 | 6.03e-28 | 104 |
| Msa1395530 | AT5G37770.2 | 42.767 | 159 | 77 | 3 | 63 | 221 | 16 | 160 | 6.03e-28 | 104 |
| Msa1395530 | AT4G20780.1 | 36.898 | 187 | 106 | 3 | 38 | 217 | 3 | 184 | 1.36e-27 | 104 |
| Msa1395530 | AT1G18210.2 | 38.710 | 155 | 73 | 2 | 62 | 216 | 21 | 153 | 3.57e-26 | 100 |
Find 50 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGATTGATGATATTGT+TGG | 0.394302 | tig0021948:+45536 | Msa1395530:CDS |
| AAAGTTGATATGGATGGAGA+TGG | 0.588783 | tig0021948:+45833 | Msa1395530:CDS |
| AAGAGAATGATGAAGAATGG+GGG | 0.685392 | tig0021948:+45878 | Msa1395530:CDS |
| AAGAGTGTAAAGAGATGATT+AGG | 0.368467 | tig0021948:+45810 | Msa1395530:CDS |
| AAGAGTTGAAGGAATCATTA+AGG | 0.371802 | tig0021948:+45489 | Msa1395530:CDS |
| AAGGAACATAAGGATCTTCA+TGG | 0.444129 | tig0021948:+45508 | Msa1395530:CDS |
| AAGGGATGCTAATTATGGAA+AGG | 0.607934 | tig0021948:+45406 | Msa1395530:CDS |
| ACAAATATTACTATTGTTAG+TGG | 0.337977 | tig0021948:-45351 | Msa1395530:intergenic |
| ACTAATGAGTTTATGGGAGG+GGG | 0.514679 | tig0021948:+45614 | Msa1395530:CDS |
| ACTTTCGACAAGAATGGTGA+TGG | 0.517448 | tig0021948:+45452 | Msa1395530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAGTTGAAGGAATCATTA+AGG | + | tig0021948:45489-45508 | Msa1395530:CDS | 30.0% | |
| AGGAATCATTAAGGAACATA+AGG | + | tig0021948:45498-45517 | Msa1395530:CDS | 30.0% | |
| AGATGAATTGGAAGAATTAG+AGG | + | tig0021948:45673-45692 | Msa1395530:CDS | 30.0% | |
| ATTAGAGGCTAATTTAAAGG+AGG | + | tig0021948:45688-45707 | Msa1395530:CDS | 30.0% | |
| GTGTTTGACAAAGATAATGA+TGG | + | tig0021948:45719-45738 | Msa1395530:CDS | 30.0% | |
| TTCAAGAGAATGATGAAGAA+TGG | + | tig0021948:45875-45894 | Msa1395530:CDS | 30.0% | |
| TCAAGAGAATGATGAAGAAT+GGG | + | tig0021948:45876-45895 | Msa1395530:CDS | 30.0% | |
| TTGGAAAGGGATGCTAATTA+TGG | + | tig0021948:45401-45420 | Msa1395530:CDS | 35.0% | |
| AAGGGATGCTAATTATGGAA+AGG | + | tig0021948:45406-45425 | Msa1395530:CDS | 35.0% | |
| CATAACAAAGCAAGAGTTGA+AGG | + | tig0021948:45478-45497 | Msa1395530:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021948 | gene | 45242 | 45922 | 45242 | ID=Msa1395530;Name=Msa1395530 |
| tig0021948 | mRNA | 45242 | 45922 | 45242 | ID=Msa1395530-mRNA-1;Parent=Msa1395530;Name=Msa1395530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|226 |
| tig0021948 | exon | 45242 | 45922 | 45242 | ID=Msa1395530-mRNA-1:exon:14917;Parent=Msa1395530-mRNA-1 |
| tig0021948 | CDS | 45242 | 45922 | 45242 | ID=Msa1395530-mRNA-1:cds;Parent=Msa1395530-mRNA-1 |
| Gene Sequence |
>Msa1395530 ATGGTTCTTGCTACACTTTTGCTAGTGGTTCTTTTCATTGCAGGCCTCATAAATATAATCTTTTACATACCTACCAACAAAATTCGTGCTTTTCTTCAAATCATTTTTCCCACTAACAATAGTAATATTTGTAAAACCAACGTGGCTACAACGACCAAGTTGGAAAGGGATGCTAATTATGGAAAGGAAGAACTCAAGAAAGTGTTTTCGACTTTCGACAAGAATGGTGATGGATTCATAACAAAGCAAGAGTTGAAGGAATCATTAAGGAACATAAGGATCTTCATGGACGAGAAAGAGATTGATGATATTGTTGGCAAGTTTGATTCTAATGGAGATGAGTTGATTGACTTTGATGAGTTTTGTTTGTTGACTAATGAGTTTATGGGAGGGGGTGAAGGTGAGAAAGTAGGTGGTGTTGGTAGTAAGGAAGATGAATTGGAAGAATTAGAGGCTAATTTAAAGGAGGCTTTTGATGTGTTTGACAAAGATAATGATGGGTTAATTTCAGTGGAGGAGCTAGCTTTGGTTCTTTGTTCGTTGGGATTAAGTGAAGGGAATAAGATTGAAGAGTGTAAAGAGATGATTAGGAAAGTTGATATGGATGGAGATGGTAATGTAAATTTTAATGAGTTCAAGAGAATGATGAAGAATGGGGGAGCAAAACTCTTTGCCACATAA |
| Protein sequence |
>Msa1395530 MVLATLLLVVLFIAGLINIIFYIPTNKIRAFLQIIFPTNNSNICKTNVATTTKLERDANYGKEELKKVFSTFDKNGDGFITKQELKESLRNIRIFMDEKEIDDIVGKFDSNGDELIDFDEFCLLTNEFMGGGEGEKVGGVGSKEDELEELEANLKEAFDVFDKDNDGLISVEELALVLCSLGLSEGNKIEECKEMIRKVDMDGDGNVNFNEFKRMMKNGGAKLFAT* |
