AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1081000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1081000 MtrunA17_Chr7g0267721 93.617 188 8 2 1 185 1 187 9.47e-127 354
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1081000 AT2G35900.1 46.078 204 79 7 1 185 1 192 1.08e-52 167
Msa1081000 AT2G35900.2 41.353 133 61 5 59 185 26 147 1.92e-23 90.9
Msa1081000 AT2G35900.3 41.353 133 61 5 59 185 26 147 1.92e-23 90.9

Find 66 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAACAAAACACACAAAATT+TGG 0.191025 7_2:+80714881 Msa1081000:five_prime_UTR
ACTGAGAAACACCTTATTAA+AGG 0.248702 7_2:-80716875 None:intergenic
AAATTTGGAAGCTAAGAAAA+TGG 0.261330 7_2:+80714896 Msa1081000:exon
GTCGATGGATAAATAATATT+TGG 0.265015 7_2:+80717054 Msa1081000:three_prime_UTR
GATTCCGTATCCTTGGATTT+AGG 0.265695 7_2:-80716738 None:intergenic
TACAAGTATGCCCTTTAATA+AGG 0.310406 7_2:+80716864 Msa1081000:three_prime_UTR
CGCAACCTCTTCGGTGGTCT+CGG 0.314056 7_2:+80716576 Msa1081000:CDS
CTGAGATTTCACGACTTTCC+TGG 0.320318 7_2:-80714982 None:intergenic
CCTCCTCACTAGGTCGGTTA+TGG 0.324513 7_2:-80714836 None:intergenic
TATCTTTATGCCAGTTGCTT+AGG 0.364991 7_2:+80716897 Msa1081000:three_prime_UTR
GATAACAACAACTCTTCTTC+AGG 0.370020 7_2:+80714939 Msa1081000:CDS
CTAAGAAAATGGGTTGGGTT+TGG 0.371297 7_2:+80714907 Msa1081000:exon
GGGATACCTATTGAAGATAA+TGG 0.379651 7_2:+80716699 Msa1081000:CDS
AGAGGAGGGTCTGCCACATT+TGG 0.396459 7_2:+80716480 Msa1081000:CDS
ATTTAGGATGAGTTTCTTGC+CGG 0.398851 7_2:-80716722 None:intergenic
TTTGATTGCAGCACTTCAAC+AGG 0.402118 7_2:-80716417 None:intergenic
CTGAGAAACACCTTATTAAA+GGG 0.416865 7_2:-80716874 None:intergenic
AGTTTGCAATAACATCAAAA+AGG 0.435162 7_2:-80716635 None:intergenic
ATCAGCTGATTCCGTATCCT+TGG 0.436734 7_2:-80716745 None:intergenic
GGAAGCTAAGAAAATGGGTT+GGG 0.440868 7_2:+80714902 Msa1081000:exon
AAAGGGTGGTTTCAATAACT+TGG 0.443116 7_2:-80714805 None:intergenic
GTCATGGAGCGCAACCTCTT+CGG 0.456959 7_2:+80716567 Msa1081000:CDS
TGCCGGCCATTATCTTCAAT+AGG 0.466167 7_2:-80716705 None:intergenic
CATGGAATGTTCAACTTCCC+TGG 0.467653 7_2:+80716525 Msa1081000:CDS
CATCTCTGCATCAAATATAG+AGG 0.468284 7_2:-80716661 None:intergenic
AGAAAACAAACCTAAGCAAC+TGG 0.469061 7_2:-80716907 None:intergenic
TTGAACCTGGAAAATTCATT+CGG 0.473945 7_2:+80715021 Msa1081000:CDS
TAGGTATCCCTCGCCTCATA+CGG 0.477154 7_2:-80716686 None:intergenic
TGTGTTAGATCACAAACATC+TGG 0.478025 7_2:+80716839 Msa1081000:three_prime_UTR
ATCACCAGTGAGGTACTTAG+AGG 0.483893 7_2:+80716462 Msa1081000:CDS
AATTTGGAAGCTAAGAAAAT+GGG 0.483977 7_2:+80714897 Msa1081000:exon
TGATACTGATTTCTCTGCAT+TGG 0.487086 7_2:+80716764 Msa1081000:CDS
ATGCAGAGATGCCCCGTATG+AGG 0.495867 7_2:+80716673 Msa1081000:CDS
AGCTGATATGGCGTGTATCG+AGG 0.497432 7_2:+80716949 Msa1081000:three_prime_UTR
TGTTTGTGTTCCTCCTCACT+AGG 0.498721 7_2:-80714846 None:intergenic
GAGCTTCTCAAAACTTGCGC+TGG 0.511543 7_2:+80715062 Msa1081000:CDS
TTAGCTAGTTTCTCTGTCGA+TGG 0.516081 7_2:+80717039 Msa1081000:three_prime_UTR
TCAATATCACTTCGTAGCCC+AGG 0.516666 7_2:-80716543 None:intergenic
TGGAAGCTAAGAAAATGGGT+TGG 0.519769 7_2:+80714901 Msa1081000:exon
CCTGGTGGAGCATCGTTCAT+CGG 0.520580 7_2:-80714964 None:intergenic
TGTGTTCCTCCTCACTAGGT+CGG 0.521804 7_2:-80714842 None:intergenic
CCGATGAACGATGCTCCACC+AGG 0.527517 7_2:+80714964 Msa1081000:CDS
TCATCCTAAATCCAAGGATA+CGG 0.528227 7_2:+80716734 Msa1081000:CDS
AGGTATCCCTCGCCTCATAC+GGG 0.544190 7_2:-80716685 None:intergenic
CCCTCCTCTAAGTACCTCAC+TGG 0.550025 7_2:-80716466 None:intergenic
ATGGAATGTTCAACTTCCCT+GGG 0.553660 7_2:+80716526 Msa1081000:CDS
CAGGGAAGTTGAACATTCCA+TGG 0.558704 7_2:-80716524 None:intergenic
ACGAAGTGATATTGAAGTCA+TGG 0.559482 7_2:+80716551 Msa1081000:CDS
GCTGATATGGCGTGTATCGA+GGG 0.564442 7_2:+80716950 Msa1081000:three_prime_UTR
TACCTATTGAAGATAATGGC+CGG 0.564553 7_2:+80716703 Msa1081000:CDS
AGAAACTCATCCTAAATCCA+AGG 0.596218 7_2:+80716728 Msa1081000:CDS
GCTCCATAACCGACCTAGTG+AGG 0.597788 7_2:+80714833 Msa1081000:five_prime_UTR
AGATGCCCCGTATGAGGCGA+GGG 0.597898 7_2:+80716679 Msa1081000:CDS
AAAACCGAAGAAGTTGAACC+TGG 0.597931 7_2:+80715008 Msa1081000:CDS
CAATATCACTTCGTAGCCCA+GGG 0.608807 7_2:-80716542 None:intergenic
ACCAGTGAGGTACTTAGAGG+AGG 0.611706 7_2:+80716465 Msa1081000:CDS
ATCTCTGCATCAAATATAGA+GGG 0.614696 7_2:-80716660 None:intergenic
ATGGAGCGCAACCTCTTCGG+TGG 0.620855 7_2:+80716570 Msa1081000:CDS
CCAGTGAGGTACTTAGAGGA+GGG 0.628488 7_2:+80716466 Msa1081000:CDS
AGATTTCACGACTTTCCTGG+TGG 0.634477 7_2:-80714979 None:intergenic
CATAACCTGTAACCAAGTGT+AGG 0.641565 7_2:-80716985 None:intergenic
TCAAATAGCTCATCAGACCA+TGG 0.648267 7_2:+80716507 Msa1081000:CDS
AGCGACCGAGACCACCGAAG+AGG 0.648679 7_2:-80716581 None:intergenic
CCATAACCGACCTAGTGAGG+AGG 0.678732 7_2:+80714836 Msa1081000:five_prime_UTR
GCTATTTGATGAGCCAAATG+TGG 0.693297 7_2:-80716493 None:intergenic
AGAAGAAGATATCACCAGTG+AGG 0.746392 7_2:+80716452 Msa1081000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATACTATTTTATTTATT+AGG + chr7_2:80715148-80715167 Msa1081000:intron 10.0%
!!! TATTTTATTTATTAGGTATT+AGG + chr7_2:80715155-80715174 Msa1081000:intron 10.0%
!!! TTCATACTATTTTATTTATT+AGG + chr7_2:80715148-80715167 Msa1081000:intron 10.0%
!!! TATTTTATTTATTAGGTATT+AGG + chr7_2:80715155-80715174 Msa1081000:intron 10.0%
!! AATAATAATAAAGAAGAAGA+AGG - chr7_2:80715106-80715125 None:intergenic 15.0%
!! ATTCAAATTAAATCGTAAAA+AGG - chr7_2:80715499-80715518 None:intergenic 15.0%
!!! ATTTAATTTGAATGATCATT+TGG + chr7_2:80715506-80715525 Msa1081000:intron 15.0%
!! TATGATACTTTATATTTGAA+TGG - chr7_2:80715946-80715965 None:intergenic 15.0%
!!! TTCAATTGATTTCAATTTTT+AGG + chr7_2:80716120-80716139 Msa1081000:intron 15.0%
!!! ATTTCAATTTTTAGGTATTA+GGG + chr7_2:80716128-80716147 Msa1081000:intron 15.0%
!!! ATTATATTTTTGTGCTTTAT+TGG + chr7_2:80716247-80716266 Msa1081000:intron 15.0%
!!! TATTGGTTGATTATATTTTT+TGG + chr7_2:80716264-80716283 Msa1081000:intron 15.0%
!! AATAATAATAAAGAAGAAGA+AGG - chr7_2:80715106-80715125 None:intergenic 15.0%
!! ATTCAAATTAAATCGTAAAA+AGG - chr7_2:80715499-80715518 None:intergenic 15.0%
!!! ATTTAATTTGAATGATCATT+TGG + chr7_2:80715506-80715525 Msa1081000:intron 15.0%
!! TATGATACTTTATATTTGAA+TGG - chr7_2:80715946-80715965 None:intergenic 15.0%
!!! TTCAATTGATTTCAATTTTT+AGG + chr7_2:80716120-80716139 Msa1081000:intron 15.0%
!!! ATTTCAATTTTTAGGTATTA+GGG + chr7_2:80716128-80716147 Msa1081000:intron 15.0%
!!! ATTATATTTTTGTGCTTTAT+TGG + chr7_2:80716247-80716266 Msa1081000:intron 15.0%
!!! TATTGGTTGATTATATTTTT+TGG + chr7_2:80716264-80716283 Msa1081000:intron 15.0%
!! AAAATAGTATGAAGCAAATT+GGG - chr7_2:80715141-80715160 None:intergenic 20.0%
!! TAAAATAGTATGAAGCAAAT+TGG - chr7_2:80715142-80715161 None:intergenic 20.0%
!! ATTTATTAGGTATTAGGATT+AGG + chr7_2:80715161-80715180 Msa1081000:intron 20.0%
!!! GGGATTTTTAATCAATTTTT+TGG + chr7_2:80715189-80715208 Msa1081000:intron 20.0%
!! TATAGTATAGGATGTTATAA+TGG - chr7_2:80715359-80715378 None:intergenic 20.0%
!!! GATTTCAATTTTTAGGTATT+AGG + chr7_2:80716127-80716146 Msa1081000:intron 20.0%
!! TGAATATCAAGATAATTTGA+TGG - chr7_2:80716188-80716207 None:intergenic 20.0%
!!! AAAAAGTTTTTGTGCTTTAT+TGG + chr7_2:80716219-80716238 Msa1081000:intron 20.0%
!! AAAATAGTATGAAGCAAATT+GGG - chr7_2:80715141-80715160 None:intergenic 20.0%
!! TAAAATAGTATGAAGCAAAT+TGG - chr7_2:80715142-80715161 None:intergenic 20.0%
!! ATTTATTAGGTATTAGGATT+AGG + chr7_2:80715161-80715180 Msa1081000:intron 20.0%
!!! GGGATTTTTAATCAATTTTT+TGG + chr7_2:80715189-80715208 Msa1081000:intron 20.0%
!! TATAGTATAGGATGTTATAA+TGG - chr7_2:80715359-80715378 None:intergenic 20.0%
!!! GATTTCAATTTTTAGGTATT+AGG + chr7_2:80716127-80716146 Msa1081000:intron 20.0%
!! TGAATATCAAGATAATTTGA+TGG - chr7_2:80716188-80716207 None:intergenic 20.0%
!!! AAAAAGTTTTTGTGCTTTAT+TGG + chr7_2:80716219-80716238 Msa1081000:intron 20.0%
! AAATTTGGAAGCTAAGAAAA+TGG + chr7_2:80714896-80714915 Msa1081000:exon 25.0%
! AATTTGGAAGCTAAGAAAAT+GGG + chr7_2:80714897-80714916 Msa1081000:exon 25.0%
! GACTTCATAAAGTATAGTAT+AGG - chr7_2:80715371-80715390 None:intergenic 25.0%
!! AAACATTGTCAATTTTGTCT+TGG - chr7_2:80715611-80715630 None:intergenic 25.0%
!!! TTTAGTGACTTGTTTTGTAT+AGG + chr7_2:80715846-80715865 Msa1081000:intron 25.0%
!!! TTAGTGACTTGTTTTGTATA+GGG + chr7_2:80715847-80715866 Msa1081000:intron 25.0%
! AAGTATCATAATATGACATG+TGG + chr7_2:80715956-80715975 Msa1081000:intron 25.0%
! ATTGAAATCAATTGCAGTAT+TGG - chr7_2:80716020-80716039 None:intergenic 25.0%
! TATCTTGATGTTGATACAAA+TGG + chr7_2:80716097-80716116 Msa1081000:intron 25.0%
!!! TTTTTGGTCATTATGTTCTT+CGG + chr7_2:80716280-80716299 Msa1081000:intron 25.0%
! TCTAATTTCATCTTTACCTT+TGG + chr7_2:80716381-80716400 Msa1081000:intron 25.0%
! AGTTTGCAATAACATCAAAA+AGG - chr7_2:80716638-80716657 None:intergenic 25.0%
! ATTAAAAACTCTAGCTGATA+TGG + chr7_2:80716937-80716956 Msa1081000:three_prime_UTR 25.0%
!! TTTTATCTTTTACCTACACT+TGG + chr7_2:80716973-80716992 Msa1081000:three_prime_UTR 25.0%
! GTCGATGGATAAATAATATT+TGG + chr7_2:80717054-80717073 Msa1081000:three_prime_UTR 25.0%
! AAATTTGGAAGCTAAGAAAA+TGG + chr7_2:80714896-80714915 Msa1081000:exon 25.0%
! AATTTGGAAGCTAAGAAAAT+GGG + chr7_2:80714897-80714916 Msa1081000:exon 25.0%
! GACTTCATAAAGTATAGTAT+AGG - chr7_2:80715371-80715390 None:intergenic 25.0%
!! AAACATTGTCAATTTTGTCT+TGG - chr7_2:80715611-80715630 None:intergenic 25.0%
!!! TTTAGTGACTTGTTTTGTAT+AGG + chr7_2:80715846-80715865 Msa1081000:intron 25.0%
!!! TTAGTGACTTGTTTTGTATA+GGG + chr7_2:80715847-80715866 Msa1081000:intron 25.0%
! AAGTATCATAATATGACATG+TGG + chr7_2:80715956-80715975 Msa1081000:intron 25.0%
! ATTGAAATCAATTGCAGTAT+TGG - chr7_2:80716020-80716039 None:intergenic 25.0%
! TATCTTGATGTTGATACAAA+TGG + chr7_2:80716097-80716116 Msa1081000:intron 25.0%
!!! TTTTTGGTCATTATGTTCTT+CGG + chr7_2:80716280-80716299 Msa1081000:intron 25.0%
! TCTAATTTCATCTTTACCTT+TGG + chr7_2:80716381-80716400 Msa1081000:intron 25.0%
! AGTTTGCAATAACATCAAAA+AGG - chr7_2:80716638-80716657 None:intergenic 25.0%
! ATTAAAAACTCTAGCTGATA+TGG + chr7_2:80716937-80716956 Msa1081000:three_prime_UTR 25.0%
!! TTTTATCTTTTACCTACACT+TGG + chr7_2:80716973-80716992 Msa1081000:three_prime_UTR 25.0%
! GTCGATGGATAAATAATATT+TGG + chr7_2:80717054-80717073 Msa1081000:three_prime_UTR 25.0%
CGAACAAAACACACAAAATT+TGG + chr7_2:80714881-80714900 Msa1081000:five_prime_UTR 30.0%
TTGAACCTGGAAAATTCATT+CGG + chr7_2:80715021-80715040 Msa1081000:CDS 30.0%
TATGAAGCAAATTGGGAATA+GGG - chr7_2:80715134-80715153 None:intergenic 30.0%
!! TACAAATTTTAGCACACGTT+TGG - chr7_2:80715330-80715349 None:intergenic 30.0%
TATACTTTATGAAGTCTCTG+AGG + chr7_2:80715375-80715394 Msa1081000:intron 30.0%
AAATATGCTAAAACCACCAA+AGG + chr7_2:80715445-80715464 Msa1081000:intron 30.0%
GAATGATCATTTGGATCAAT+TGG + chr7_2:80715515-80715534 Msa1081000:intron 30.0%
TCATTTGGATCAATTGGAAA+TGG + chr7_2:80715521-80715540 Msa1081000:intron 30.0%
ACTTTACCTGAAAAGAACAT+TGG + chr7_2:80715694-80715713 Msa1081000:intron 30.0%
! ATTGTACCAATGTTCTTTTC+AGG - chr7_2:80715703-80715722 None:intergenic 30.0%
TTTCGAAAATCAGGTTTCTT+GGG - chr7_2:80715824-80715843 None:intergenic 30.0%
!! TTTGTATAGGGTTGTTTTAC+AGG + chr7_2:80715859-80715878 Msa1081000:intron 30.0%
TCAACATCAAGATACTTTGA+TGG - chr7_2:80716091-80716110 None:intergenic 30.0%
! TTTTGGTCATTATGTTCTTC+GGG + chr7_2:80716281-80716300 Msa1081000:intron 30.0%
ATCTCTGCATCAAATATAGA+GGG - chr7_2:80716663-80716682 None:intergenic 30.0%
TACAAGTATGCCCTTTAATA+AGG + chr7_2:80716864-80716883 Msa1081000:three_prime_UTR 30.0%
CTGAGAAACACCTTATTAAA+GGG - chr7_2:80716877-80716896 None:intergenic 30.0%
ACTGAGAAACACCTTATTAA+AGG - chr7_2:80716878-80716897 None:intergenic 30.0%
CGAACAAAACACACAAAATT+TGG + chr7_2:80714881-80714900 Msa1081000:five_prime_UTR 30.0%
TTGAACCTGGAAAATTCATT+CGG + chr7_2:80715021-80715040 Msa1081000:CDS 30.0%
TATGAAGCAAATTGGGAATA+GGG - chr7_2:80715134-80715153 None:intergenic 30.0%
!! TACAAATTTTAGCACACGTT+TGG - chr7_2:80715330-80715349 None:intergenic 30.0%
TATACTTTATGAAGTCTCTG+AGG + chr7_2:80715375-80715394 Msa1081000:intron 30.0%
AAATATGCTAAAACCACCAA+AGG + chr7_2:80715445-80715464 Msa1081000:intron 30.0%
GAATGATCATTTGGATCAAT+TGG + chr7_2:80715515-80715534 Msa1081000:intron 30.0%
TCATTTGGATCAATTGGAAA+TGG + chr7_2:80715521-80715540 Msa1081000:intron 30.0%
ACTTTACCTGAAAAGAACAT+TGG + chr7_2:80715694-80715713 Msa1081000:intron 30.0%
! ATTGTACCAATGTTCTTTTC+AGG - chr7_2:80715703-80715722 None:intergenic 30.0%
TTTCGAAAATCAGGTTTCTT+GGG - chr7_2:80715824-80715843 None:intergenic 30.0%
!! TTTGTATAGGGTTGTTTTAC+AGG + chr7_2:80715859-80715878 Msa1081000:intron 30.0%
TCAACATCAAGATACTTTGA+TGG - chr7_2:80716091-80716110 None:intergenic 30.0%
! TTTTGGTCATTATGTTCTTC+GGG + chr7_2:80716281-80716300 Msa1081000:intron 30.0%
ATCTCTGCATCAAATATAGA+GGG - chr7_2:80716663-80716682 None:intergenic 30.0%
TACAAGTATGCCCTTTAATA+AGG + chr7_2:80716864-80716883 Msa1081000:three_prime_UTR 30.0%
CTGAGAAACACCTTATTAAA+GGG - chr7_2:80716877-80716896 None:intergenic 30.0%
ACTGAGAAACACCTTATTAA+AGG - chr7_2:80716878-80716897 None:intergenic 30.0%
AAAGGGTGGTTTCAATAACT+TGG - chr7_2:80714808-80714827 None:intergenic 35.0%
TTATGGAGCTTAAAAAAGGG+TGG - chr7_2:80714822-80714841 None:intergenic 35.0%
CGGTTATGGAGCTTAAAAAA+GGG - chr7_2:80714825-80714844 None:intergenic 35.0%
TCGGTTATGGAGCTTAAAAA+AGG - chr7_2:80714826-80714845 None:intergenic 35.0%
GATAACAACAACTCTTCTTC+AGG + chr7_2:80714939-80714958 Msa1081000:CDS 35.0%
! TTTTCCAGGTTCAACTTCTT+CGG - chr7_2:80715015-80715034 None:intergenic 35.0%
! CATTTCCGAATGAATTTTCC+AGG - chr7_2:80715029-80715048 None:intergenic 35.0%
GTATGAAGCAAATTGGGAAT+AGG - chr7_2:80715135-80715154 None:intergenic 35.0%
AGGTATTAGGATTAGGATGA+TGG + chr7_2:80715168-80715187 Msa1081000:intron 35.0%
GGTATTAGGATTAGGATGAT+GGG + chr7_2:80715169-80715188 Msa1081000:intron 35.0%
! TGATTTTGCAAAAGCTACAC+AGG - chr7_2:80715276-80715295 None:intergenic 35.0%
! ATTTTATTCTGCCAAACCGA+TGG + chr7_2:80715299-80715318 Msa1081000:intron 35.0%
AAACCACCAAAGGTTTCATT+TGG + chr7_2:80715455-80715474 Msa1081000:intron 35.0%
AGCATTAAGGATCAGAAAGT+CGG - chr7_2:80715583-80715602 None:intergenic 35.0%
CTTTCGAAAATCAGGTTTCT+TGG - chr7_2:80715825-80715844 None:intergenic 35.0%
CACTAAAGCTTTCGAAAATC+AGG - chr7_2:80715833-80715852 None:intergenic 35.0%
TGTTCTATTTGTGTAGTGTG+TGG + chr7_2:80715983-80716002 Msa1081000:intron 35.0%
GTTCTATTTGTGTAGTGTGT+GGG + chr7_2:80715984-80716003 Msa1081000:intron 35.0%
!! AAATCAATTGCAGTATTGGC+AGG - chr7_2:80716016-80716035 None:intergenic 35.0%
GATGAAACAGGTACACAAAA+AGG - chr7_2:80716331-80716350 None:intergenic 35.0%
TTACCTTTGGACTAATTCTC+AGG + chr7_2:80716394-80716413 Msa1081000:intron 35.0%
ACGAAGTGATATTGAAGTCA+TGG + chr7_2:80716551-80716570 Msa1081000:CDS 35.0%
GCAGCTGAAGAAATGAAAAA+TGG + chr7_2:80716612-80716631 Msa1081000:CDS 35.0%
CATCTCTGCATCAAATATAG+AGG - chr7_2:80716664-80716683 None:intergenic 35.0%
GGGATACCTATTGAAGATAA+TGG + chr7_2:80716699-80716718 Msa1081000:CDS 35.0%
! TACCTATTGAAGATAATGGC+CGG + chr7_2:80716703-80716722 Msa1081000:CDS 35.0%
! ATTTAGGATGAGTTTCTTGC+CGG - chr7_2:80716725-80716744 None:intergenic 35.0%
AGAAACTCATCCTAAATCCA+AGG + chr7_2:80716728-80716747 Msa1081000:CDS 35.0%
TCATCCTAAATCCAAGGATA+CGG + chr7_2:80716734-80716753 Msa1081000:CDS 35.0%
!! TGATACTGATTTCTCTGCAT+TGG + chr7_2:80716764-80716783 Msa1081000:CDS 35.0%
TGTGTTAGATCACAAACATC+TGG + chr7_2:80716839-80716858 Msa1081000:three_prime_UTR 35.0%
TATCTTTATGCCAGTTGCTT+AGG + chr7_2:80716897-80716916 Msa1081000:three_prime_UTR 35.0%
AGAAAACAAACCTAAGCAAC+TGG - chr7_2:80716910-80716929 None:intergenic 35.0%
! TTTTACCTACACTTGGTTAC+AGG + chr7_2:80716980-80716999 Msa1081000:three_prime_UTR 35.0%
AAAGGGTGGTTTCAATAACT+TGG - chr7_2:80714808-80714827 None:intergenic 35.0%
TTATGGAGCTTAAAAAAGGG+TGG - chr7_2:80714822-80714841 None:intergenic 35.0%
CGGTTATGGAGCTTAAAAAA+GGG - chr7_2:80714825-80714844 None:intergenic 35.0%
TCGGTTATGGAGCTTAAAAA+AGG - chr7_2:80714826-80714845 None:intergenic 35.0%
GATAACAACAACTCTTCTTC+AGG + chr7_2:80714939-80714958 Msa1081000:CDS 35.0%
! TTTTCCAGGTTCAACTTCTT+CGG - chr7_2:80715015-80715034 None:intergenic 35.0%
! CATTTCCGAATGAATTTTCC+AGG - chr7_2:80715029-80715048 None:intergenic 35.0%
GTATGAAGCAAATTGGGAAT+AGG - chr7_2:80715135-80715154 None:intergenic 35.0%
AGGTATTAGGATTAGGATGA+TGG + chr7_2:80715168-80715187 Msa1081000:intron 35.0%
GGTATTAGGATTAGGATGAT+GGG + chr7_2:80715169-80715188 Msa1081000:intron 35.0%
! TGATTTTGCAAAAGCTACAC+AGG - chr7_2:80715276-80715295 None:intergenic 35.0%
! ATTTTATTCTGCCAAACCGA+TGG + chr7_2:80715299-80715318 Msa1081000:intron 35.0%
AAACCACCAAAGGTTTCATT+TGG + chr7_2:80715455-80715474 Msa1081000:intron 35.0%
AGCATTAAGGATCAGAAAGT+CGG - chr7_2:80715583-80715602 None:intergenic 35.0%
CTTTCGAAAATCAGGTTTCT+TGG - chr7_2:80715825-80715844 None:intergenic 35.0%
CACTAAAGCTTTCGAAAATC+AGG - chr7_2:80715833-80715852 None:intergenic 35.0%
TGTTCTATTTGTGTAGTGTG+TGG + chr7_2:80715983-80716002 Msa1081000:intron 35.0%
GTTCTATTTGTGTAGTGTGT+GGG + chr7_2:80715984-80716003 Msa1081000:intron 35.0%
!! AAATCAATTGCAGTATTGGC+AGG - chr7_2:80716016-80716035 None:intergenic 35.0%
GATGAAACAGGTACACAAAA+AGG - chr7_2:80716331-80716350 None:intergenic 35.0%
TTACCTTTGGACTAATTCTC+AGG + chr7_2:80716394-80716413 Msa1081000:intron 35.0%
ACGAAGTGATATTGAAGTCA+TGG + chr7_2:80716551-80716570 Msa1081000:CDS 35.0%
GCAGCTGAAGAAATGAAAAA+TGG + chr7_2:80716612-80716631 Msa1081000:CDS 35.0%
CATCTCTGCATCAAATATAG+AGG - chr7_2:80716664-80716683 None:intergenic 35.0%
GGGATACCTATTGAAGATAA+TGG + chr7_2:80716699-80716718 Msa1081000:CDS 35.0%
! TACCTATTGAAGATAATGGC+CGG + chr7_2:80716703-80716722 Msa1081000:CDS 35.0%
! ATTTAGGATGAGTTTCTTGC+CGG - chr7_2:80716725-80716744 None:intergenic 35.0%
AGAAACTCATCCTAAATCCA+AGG + chr7_2:80716728-80716747 Msa1081000:CDS 35.0%
TCATCCTAAATCCAAGGATA+CGG + chr7_2:80716734-80716753 Msa1081000:CDS 35.0%
!! TGATACTGATTTCTCTGCAT+TGG + chr7_2:80716764-80716783 Msa1081000:CDS 35.0%
TGTGTTAGATCACAAACATC+TGG + chr7_2:80716839-80716858 Msa1081000:three_prime_UTR 35.0%
TATCTTTATGCCAGTTGCTT+AGG + chr7_2:80716897-80716916 Msa1081000:three_prime_UTR 35.0%
AGAAAACAAACCTAAGCAAC+TGG - chr7_2:80716910-80716929 None:intergenic 35.0%
! TTTTACCTACACTTGGTTAC+AGG + chr7_2:80716980-80716999 Msa1081000:three_prime_UTR 35.0%
TGGAAGCTAAGAAAATGGGT+TGG + chr7_2:80714901-80714920 Msa1081000:exon 40.0%
! GGAAGCTAAGAAAATGGGTT+GGG + chr7_2:80714902-80714921 Msa1081000:exon 40.0%
!! CTAAGAAAATGGGTTGGGTT+TGG + chr7_2:80714907-80714926 Msa1081000:exon 40.0%
AAAACCGAAGAAGTTGAACC+TGG + chr7_2:80715008-80715027 Msa1081000:CDS 40.0%
ATCGCTCCAAATGAAACCTT+TGG - chr7_2:80715464-80715483 None:intergenic 40.0%
! TGTCTTGGCATAGAGCATTA+AGG - chr7_2:80715596-80715615 None:intergenic 40.0%
!! GGTTGTTTTACAGGCTTTGA+AGG + chr7_2:80715868-80715887 Msa1081000:intron 40.0%
TAGGTATTAGGGTTATGCAC+TGG + chr7_2:80716139-80716158 Msa1081000:intron 40.0%
TTTGATTGCAGCACTTCAAC+AGG - chr7_2:80716420-80716439 None:intergenic 40.0%
AGAAGAAGATATCACCAGTG+AGG + chr7_2:80716452-80716471 Msa1081000:CDS 40.0%
GCTATTTGATGAGCCAAATG+TGG - chr7_2:80716496-80716515 None:intergenic 40.0%
TCAAATAGCTCATCAGACCA+TGG + chr7_2:80716507-80716526 Msa1081000:CDS 40.0%
ATGGAATGTTCAACTTCCCT+GGG + chr7_2:80716526-80716545 Msa1081000:CDS 40.0%
GATTCCGTATCCTTGGATTT+AGG - chr7_2:80716741-80716760 None:intergenic 40.0%
CATAACCTGTAACCAAGTGT+AGG - chr7_2:80716988-80717007 None:intergenic 40.0%
! TTAGCTAGTTTCTCTGTCGA+TGG + chr7_2:80717039-80717058 Msa1081000:three_prime_UTR 40.0%
TGGAAGCTAAGAAAATGGGT+TGG + chr7_2:80714901-80714920 Msa1081000:exon 40.0%
! GGAAGCTAAGAAAATGGGTT+GGG + chr7_2:80714902-80714921 Msa1081000:exon 40.0%
!! CTAAGAAAATGGGTTGGGTT+TGG + chr7_2:80714907-80714926 Msa1081000:exon 40.0%
AAAACCGAAGAAGTTGAACC+TGG + chr7_2:80715008-80715027 Msa1081000:CDS 40.0%
ATCGCTCCAAATGAAACCTT+TGG - chr7_2:80715464-80715483 None:intergenic 40.0%
! TGTCTTGGCATAGAGCATTA+AGG - chr7_2:80715596-80715615 None:intergenic 40.0%
!! GGTTGTTTTACAGGCTTTGA+AGG + chr7_2:80715868-80715887 Msa1081000:intron 40.0%
TAGGTATTAGGGTTATGCAC+TGG + chr7_2:80716139-80716158 Msa1081000:intron 40.0%
TTTGATTGCAGCACTTCAAC+AGG - chr7_2:80716420-80716439 None:intergenic 40.0%
AGAAGAAGATATCACCAGTG+AGG + chr7_2:80716452-80716471 Msa1081000:CDS 40.0%
GCTATTTGATGAGCCAAATG+TGG - chr7_2:80716496-80716515 None:intergenic 40.0%
TCAAATAGCTCATCAGACCA+TGG + chr7_2:80716507-80716526 Msa1081000:CDS 40.0%
ATGGAATGTTCAACTTCCCT+GGG + chr7_2:80716526-80716545 Msa1081000:CDS 40.0%
GATTCCGTATCCTTGGATTT+AGG - chr7_2:80716741-80716760 None:intergenic 40.0%
CATAACCTGTAACCAAGTGT+AGG - chr7_2:80716988-80717007 None:intergenic 40.0%
! TTAGCTAGTTTCTCTGTCGA+TGG + chr7_2:80717039-80717058 Msa1081000:three_prime_UTR 40.0%
TGTTTGTGTTCCTCCTCACT+AGG - chr7_2:80714849-80714868 None:intergenic 45.0%
AGATTTCACGACTTTCCTGG+TGG - chr7_2:80714982-80715001 None:intergenic 45.0%
CTGAGATTTCACGACTTTCC+TGG - chr7_2:80714985-80715004 None:intergenic 45.0%
!! GTTTGGATTAGCCATCGGTT+TGG - chr7_2:80715313-80715332 None:intergenic 45.0%
!! CACACGTTTGGATTAGCCAT+CGG - chr7_2:80715318-80715337 None:intergenic 45.0%
GCTCCAAATGAAACCTTTGG+TGG - chr7_2:80715461-80715480 None:intergenic 45.0%
! GTTGTCGTTTGCAACATAGC+AGG + chr7_2:80715723-80715742 Msa1081000:intron 45.0%
GCAACATAGCAGGAAGAGAA+TGG + chr7_2:80715733-80715752 Msa1081000:intron 45.0%
CATGTACATGCACAAGTGAC+AGG - chr7_2:80715761-80715780 None:intergenic 45.0%
CACTTGTGCATGTACATGTG+TGG + chr7_2:80715763-80715782 Msa1081000:intron 45.0%
!! GCACAAGTAGCACCACTTTT+TGG + chr7_2:80716058-80716077 Msa1081000:intron 45.0%
! GATGGAGCACTTCCAAAAAG+TGG - chr7_2:80716073-80716092 None:intergenic 45.0%
TTCAGTCGCATTTGCAGAGA+GGG - chr7_2:80716359-80716378 None:intergenic 45.0%
TTTCAGTCGCATTTGCAGAG+AGG - chr7_2:80716360-80716379 None:intergenic 45.0%
AGGCCTGAGAATTAGTCCAA+AGG - chr7_2:80716400-80716419 None:intergenic 45.0%
! ATCACCAGTGAGGTACTTAG+AGG + chr7_2:80716462-80716481 Msa1081000:CDS 45.0%
CAGGGAAGTTGAACATTCCA+TGG - chr7_2:80716527-80716546 None:intergenic 45.0%
CATGGAATGTTCAACTTCCC+TGG + chr7_2:80716525-80716544 Msa1081000:CDS 45.0%
CAATATCACTTCGTAGCCCA+GGG - chr7_2:80716545-80716564 None:intergenic 45.0%
TCAATATCACTTCGTAGCCC+AGG - chr7_2:80716546-80716565 None:intergenic 45.0%
TGCCGGCCATTATCTTCAAT+AGG - chr7_2:80716708-80716727 None:intergenic 45.0%
! ATCAGCTGATTCCGTATCCT+TGG - chr7_2:80716748-80716767 None:intergenic 45.0%
TGTTTGTGTTCCTCCTCACT+AGG - chr7_2:80714849-80714868 None:intergenic 45.0%
AGATTTCACGACTTTCCTGG+TGG - chr7_2:80714982-80715001 None:intergenic 45.0%
CTGAGATTTCACGACTTTCC+TGG - chr7_2:80714985-80715004 None:intergenic 45.0%
!! GTTTGGATTAGCCATCGGTT+TGG - chr7_2:80715313-80715332 None:intergenic 45.0%
!! CACACGTTTGGATTAGCCAT+CGG - chr7_2:80715318-80715337 None:intergenic 45.0%
GCTCCAAATGAAACCTTTGG+TGG - chr7_2:80715461-80715480 None:intergenic 45.0%
! GTTGTCGTTTGCAACATAGC+AGG + chr7_2:80715723-80715742 Msa1081000:intron 45.0%
GCAACATAGCAGGAAGAGAA+TGG + chr7_2:80715733-80715752 Msa1081000:intron 45.0%
CATGTACATGCACAAGTGAC+AGG - chr7_2:80715761-80715780 None:intergenic 45.0%
CACTTGTGCATGTACATGTG+TGG + chr7_2:80715763-80715782 Msa1081000:intron 45.0%
!! GCACAAGTAGCACCACTTTT+TGG + chr7_2:80716058-80716077 Msa1081000:intron 45.0%
! GATGGAGCACTTCCAAAAAG+TGG - chr7_2:80716073-80716092 None:intergenic 45.0%
TTCAGTCGCATTTGCAGAGA+GGG - chr7_2:80716359-80716378 None:intergenic 45.0%
TTTCAGTCGCATTTGCAGAG+AGG - chr7_2:80716360-80716379 None:intergenic 45.0%
AGGCCTGAGAATTAGTCCAA+AGG - chr7_2:80716400-80716419 None:intergenic 45.0%
! ATCACCAGTGAGGTACTTAG+AGG + chr7_2:80716462-80716481 Msa1081000:CDS 45.0%
CAGGGAAGTTGAACATTCCA+TGG - chr7_2:80716527-80716546 None:intergenic 45.0%
CATGGAATGTTCAACTTCCC+TGG + chr7_2:80716525-80716544 Msa1081000:CDS 45.0%
CAATATCACTTCGTAGCCCA+GGG - chr7_2:80716545-80716564 None:intergenic 45.0%
TCAATATCACTTCGTAGCCC+AGG - chr7_2:80716546-80716565 None:intergenic 45.0%
TGCCGGCCATTATCTTCAAT+AGG - chr7_2:80716708-80716727 None:intergenic 45.0%
! ATCAGCTGATTCCGTATCCT+TGG - chr7_2:80716748-80716767 None:intergenic 45.0%
TGTGTTCCTCCTCACTAGGT+CGG - chr7_2:80714845-80714864 None:intergenic 50.0%
GAGCTTCTCAAAACTTGCGC+TGG + chr7_2:80715062-80715081 Msa1081000:CDS 50.0%
! AGTGAGTGTCGCTGCATGTT+GGG - chr7_2:80715908-80715927 None:intergenic 50.0%
! ACCAGTGAGGTACTTAGAGG+AGG + chr7_2:80716465-80716484 Msa1081000:CDS 50.0%
! CCAGTGAGGTACTTAGAGGA+GGG + chr7_2:80716466-80716485 Msa1081000:CDS 50.0%
TAGGTATCCCTCGCCTCATA+CGG - chr7_2:80716689-80716708 None:intergenic 50.0%
AGCTGATATGGCGTGTATCG+AGG + chr7_2:80716949-80716968 Msa1081000:three_prime_UTR 50.0%
GCTGATATGGCGTGTATCGA+GGG + chr7_2:80716950-80716969 Msa1081000:three_prime_UTR 50.0%
TGTGTTCCTCCTCACTAGGT+CGG - chr7_2:80714845-80714864 None:intergenic 50.0%
GAGCTTCTCAAAACTTGCGC+TGG + chr7_2:80715062-80715081 Msa1081000:CDS 50.0%
! AGTGAGTGTCGCTGCATGTT+GGG - chr7_2:80715908-80715927 None:intergenic 50.0%
! ACCAGTGAGGTACTTAGAGG+AGG + chr7_2:80716465-80716484 Msa1081000:CDS 50.0%
! CCAGTGAGGTACTTAGAGGA+GGG + chr7_2:80716466-80716485 Msa1081000:CDS 50.0%
TAGGTATCCCTCGCCTCATA+CGG - chr7_2:80716689-80716708 None:intergenic 50.0%
AGCTGATATGGCGTGTATCG+AGG + chr7_2:80716949-80716968 Msa1081000:three_prime_UTR 50.0%
GCTGATATGGCGTGTATCGA+GGG + chr7_2:80716950-80716969 Msa1081000:three_prime_UTR 50.0%
GCTCCATAACCGACCTAGTG+AGG + chr7_2:80714833-80714852 Msa1081000:five_prime_UTR 55.0%
CCTCCTCACTAGGTCGGTTA+TGG - chr7_2:80714839-80714858 None:intergenic 55.0%
CCATAACCGACCTAGTGAGG+AGG + chr7_2:80714836-80714855 Msa1081000:five_prime_UTR 55.0%
!! CCTGGTGGAGCATCGTTCAT+CGG - chr7_2:80714967-80714986 None:intergenic 55.0%
! CAGTGAGTGTCGCTGCATGT+TGG - chr7_2:80715909-80715928 None:intergenic 55.0%
GAGAGGGTGGGTGATGAAAC+AGG - chr7_2:80716343-80716362 None:intergenic 55.0%
GTCGCATTTGCAGAGAGGGT+GGG - chr7_2:80716355-80716374 None:intergenic 55.0%
AGTCGCATTTGCAGAGAGGG+TGG - chr7_2:80716356-80716375 None:intergenic 55.0%
CCCTCCTCTAAGTACCTCAC+TGG - chr7_2:80716469-80716488 None:intergenic 55.0%
AGAGGAGGGTCTGCCACATT+TGG + chr7_2:80716480-80716499 Msa1081000:CDS 55.0%
GTCATGGAGCGCAACCTCTT+CGG + chr7_2:80716567-80716586 Msa1081000:CDS 55.0%
ATGCAGAGATGCCCCGTATG+AGG + chr7_2:80716673-80716692 Msa1081000:CDS 55.0%
AGGTATCCCTCGCCTCATAC+GGG - chr7_2:80716688-80716707 None:intergenic 55.0%
GCTCCATAACCGACCTAGTG+AGG + chr7_2:80714833-80714852 Msa1081000:five_prime_UTR 55.0%
CCTCCTCACTAGGTCGGTTA+TGG - chr7_2:80714839-80714858 None:intergenic 55.0%
CCATAACCGACCTAGTGAGG+AGG + chr7_2:80714836-80714855 Msa1081000:five_prime_UTR 55.0%
!! CCTGGTGGAGCATCGTTCAT+CGG - chr7_2:80714967-80714986 None:intergenic 55.0%
! CAGTGAGTGTCGCTGCATGT+TGG - chr7_2:80715909-80715928 None:intergenic 55.0%
GAGAGGGTGGGTGATGAAAC+AGG - chr7_2:80716343-80716362 None:intergenic 55.0%
GTCGCATTTGCAGAGAGGGT+GGG - chr7_2:80716355-80716374 None:intergenic 55.0%
AGTCGCATTTGCAGAGAGGG+TGG - chr7_2:80716356-80716375 None:intergenic 55.0%
CCCTCCTCTAAGTACCTCAC+TGG - chr7_2:80716469-80716488 None:intergenic 55.0%
AGAGGAGGGTCTGCCACATT+TGG + chr7_2:80716480-80716499 Msa1081000:CDS 55.0%
GTCATGGAGCGCAACCTCTT+CGG + chr7_2:80716567-80716586 Msa1081000:CDS 55.0%
ATGCAGAGATGCCCCGTATG+AGG + chr7_2:80716673-80716692 Msa1081000:CDS 55.0%
AGGTATCCCTCGCCTCATAC+GGG - chr7_2:80716688-80716707 None:intergenic 55.0%
CCGATGAACGATGCTCCACC+AGG + chr7_2:80714964-80714983 Msa1081000:CDS 60.0%
ATGGAGCGCAACCTCTTCGG+TGG + chr7_2:80716570-80716589 Msa1081000:CDS 60.0%
CGCAACCTCTTCGGTGGTCT+CGG + chr7_2:80716576-80716595 Msa1081000:CDS 60.0%
AGATGCCCCGTATGAGGCGA+GGG + chr7_2:80716679-80716698 Msa1081000:CDS 60.0%
GGTATCCCTCGCCTCATACG+GGG - chr7_2:80716687-80716706 None:intergenic 60.0%
CCGATGAACGATGCTCCACC+AGG + chr7_2:80714964-80714983 Msa1081000:CDS 60.0%
ATGGAGCGCAACCTCTTCGG+TGG + chr7_2:80716570-80716589 Msa1081000:CDS 60.0%
CGCAACCTCTTCGGTGGTCT+CGG + chr7_2:80716576-80716595 Msa1081000:CDS 60.0%
AGATGCCCCGTATGAGGCGA+GGG + chr7_2:80716679-80716698 Msa1081000:CDS 60.0%
GGTATCCCTCGCCTCATACG+GGG - chr7_2:80716687-80716706 None:intergenic 60.0%
AGCGACCGAGACCACCGAAG+AGG - chr7_2:80716584-80716603 None:intergenic 65.0%
GAGATGCCCCGTATGAGGCG+AGG + chr7_2:80716678-80716697 Msa1081000:CDS 65.0%
AGCGACCGAGACCACCGAAG+AGG - chr7_2:80716584-80716603 None:intergenic 65.0%
GAGATGCCCCGTATGAGGCG+AGG + chr7_2:80716678-80716697 Msa1081000:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 80714773 80717077 80714773 ID=Msa1081000;Name=Msa1081000
chr7_2 mRNA 80714773 80717077 80714773 ID=Msa1081000-mRNA-1;Parent=Msa1081000;Name=Msa1081000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=142|1|1|1|1|1|2|277|185
chr7_2 exon 80714773 80715087 80714773 ID=Msa1081000-mRNA-1:exon:20482;Parent=Msa1081000-mRNA-1
chr7_2 exon 80716416 80717077 80716416 ID=Msa1081000-mRNA-1:exon:20483;Parent=Msa1081000-mRNA-1
chr7_2 five_prime_UTR 80714773 80714914 80714773 ID=Msa1081000-mRNA-1:five_prime_utr;Parent=Msa1081000-mRNA-1
chr7_2 CDS 80714915 80715087 80714915 ID=Msa1081000-mRNA-1:cds;Parent=Msa1081000-mRNA-1
chr7_2 CDS 80716416 80716800 80716416 ID=Msa1081000-mRNA-1:cds;Parent=Msa1081000-mRNA-1
chr7_2 three_prime_UTR 80716801 80717077 80716801 ID=Msa1081000-mRNA-1:three_prime_utr;Parent=Msa1081000-mRNA-1
Gene Sequence

>Msa1081000

ATGGGTTGGGTTTGGAGCGACGACGATAACAACAACTCTTCTTCAGGTTCCGATGAACGATGCTCCACCAGGAAAGTCGTGAAATCTCAGTGCAAAACCGAAGAAGTTGAACCTGGAAAATTCATTCGGAAATGCGAGAAAACTGAAGAGCTTCTCAAAACTTGCGCTGGAAAGCCTGTTGAAGTGCTGCAATCAAACAAAGAGTACACAGAAGAAGATATCACCAGTGAGGTACTTAGAGGAGGGTCTGCCACATTTGGCTCATCAAATAGCTCATCAGACCATGGAATGTTCAACTTCCCTGGGCTACGAAGTGATATTGAAGTCATGGAGCGCAACCTCTTCGGTGGTCTCGGTCGCTTCTTTGAAGCAGCTGAAGAAATGAAAAATGGCCTTTTTGATGTTATTGCAAACTCTCCCTCTATATTTGATGCAGAGATGCCCCGTATGAGGCGAGGGATACCTATTGAAGATAATGGCCGGCAAGAAACTCATCCTAAATCCAAGGATACGGAATCAGCTGATACTGATTTCTCTGCATTGGCTAAAGATGTTTAA
Protein sequence

>Msa1081000

MGWVWSDDDNNNSSSGSDERCSTRKVVKSQCKTEEVEPGKFIRKCEKTEELLKTCAGKPVEVLQSNKEYTEEDITSEVLRGGSATFGSSNSSSDHGMFNFPGLRSDIEVMERNLFGGLGRFFEAAEEMKNGLFDVIANSPSIFDAEMPRMRRGIPIEDNGRQETHPKSKDTESADTDFSALAKDV*