AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1287360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1287360 MtrunA17_Chr8g0360861 98.165 109 2 0 1 109 1 109 1.12e-76 221
Msa1287360 MtrunA17_Chr6g0485891 50.538 93 46 0 1 93 35 127 8.05e-30 103
Msa1287360 MtrunA17_Chr2g0331651 55.556 90 40 0 1 90 1 90 1.56e-29 101
Msa1287360 MtrunA17_Chr4g0067531 48.837 86 42 1 5 90 46 129 6.24e-26 94.0
Msa1287360 MtrunA17_Chr4g0063491 49.367 79 40 0 11 89 159 237 9.14e-22 85.9
Msa1287360 MtrunA17_Chr8g0391451 45.882 85 44 1 12 94 60 144 3.09e-19 77.0
Msa1287360 MtrunA17_Chr4g0059791 50.633 79 38 1 12 89 222 300 2.64e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1287360 AT4G27360.1 70.874 103 30 0 1 103 1 103 1.33e-49 153
Msa1287360 AT3G16120.2 55.435 92 41 0 1 92 1 92 8.81e-33 110
Msa1287360 AT3G16120.1 55.435 92 41 0 1 92 1 92 8.81e-33 110
Msa1287360 AT1G52245.1 51.064 94 46 0 1 94 1 94 4.11e-30 103
Msa1287360 AT4G15930.1 49.412 85 41 1 5 89 39 121 7.44e-24 88.6
Msa1287360 AT5G20110.1 46.988 83 44 0 12 94 123 205 5.24e-21 83.6
Msa1287360 AT1G23220.1 39.773 88 52 1 8 94 38 125 5.70e-18 73.9
Msa1287360 AT4G15930.2 49.180 61 29 1 5 65 39 97 1.87e-13 61.2

Find 29 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTATTAAAAGAAAATTTA+AGG 0.130933 8_3:+37644418 None:intergenic
TGCATTGTAGGCACTGATTT+TGG 0.221609 8_3:-37644731 Msa1287360:CDS
GCACAAGAGTAGAACAAAAT+AGG 0.295751 8_3:+37645075 None:intergenic
CAGGAATTTGATAGGGTTCA+TGG 0.298316 8_3:-37644767 Msa1287360:CDS
CAGTTACATCATAGAAATCT+AGG 0.300776 8_3:+37644912 None:intergenic
AATTATTGCAGGAATTTGAT+AGG 0.304941 8_3:-37644775 Msa1287360:intron
ATCATAGAAATCTAGGGCTT+TGG 0.353606 8_3:+37644919 None:intergenic
CTTCAATTCATCATGCTAGA+AGG 0.354098 8_3:-37645007 Msa1287360:exon
TCTATTAAAAGAAAATTTAA+GGG 0.380118 8_3:+37644419 None:intergenic
TTGCTCTTTAAGGGATCTGC+TGG 0.420352 8_3:-37644647 Msa1287360:CDS
CCTGGTTGGCAATGCATTGT+AGG 0.440795 8_3:-37644743 Msa1287360:CDS
CTAGAAGGCAAGGCAGTGAT+TGG 0.481296 8_3:-37644992 Msa1287360:CDS
TAGATTAAAGATAAAGTGAT+GGG 0.498796 8_3:+37645126 None:intergenic
CCTACAATGCATTGCCAACC+AGG 0.504627 8_3:+37644743 None:intergenic
AAATCAGTTCTCAGCAATAG+AGG 0.512873 8_3:-37644609 Msa1287360:CDS
TATAGCTCGATTTATAAAGA+AGG 0.514762 8_3:-37644879 Msa1287360:intron
TTTGATAGGGTTCATGGACC+TGG 0.522418 8_3:-37644761 Msa1287360:CDS
ATTATTGCAGGAATTTGATA+GGG 0.524356 8_3:-37644774 Msa1287360:intron
TATAAATCGAGCTATATCAA+TGG 0.561045 8_3:+37644886 None:intergenic
ATCTGCTGGTCCACAAGCAG+AGG 0.571115 8_3:-37644633 Msa1287360:CDS
GTAGATTAAAGATAAAGTGA+TGG 0.580576 8_3:+37645125 None:intergenic
ATTCATCATGCTAGAAGGCA+AGG 0.581622 8_3:-37645002 Msa1287360:exon
TCCTTTGTGACACATTGTAG+TGG 0.583602 8_3:-37644707 Msa1287360:CDS
AGTTACATCATAGAAATCTA+GGG 0.585718 8_3:+37644913 None:intergenic
ATAGGGTTCATGGACCTGGT+TGG 0.594187 8_3:-37644757 Msa1287360:CDS
GATTAAAGATAAAGTGATGG+GGG 0.605952 8_3:+37645128 None:intergenic
GCCACTACAATGTGTCACAA+AGG 0.659746 8_3:+37644706 None:intergenic
AGATTAAAGATAAAGTGATG+GGG 0.675381 8_3:+37645127 None:intergenic
AGATTTCTATGATGTAACTG+AGG 0.680263 8_3:-37644909 Msa1287360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTTGAAAAACAAAATA+TGG - chr8_3:37645095-37645114 Msa1287360:five_prime_UTR 15.0%
!! AAGATTTGAAAAACAAAATA+TGG - chr8_3:37645095-37645114 Msa1287360:five_prime_UTR 15.0%
!! TAGATTAAAGATAAAGTGAT+GGG + chr8_3:37644470-37644489 None:intergenic 20.0%
!!! ATAGGCTACTTTTTTTTTAT+TGG + chr8_3:37644503-37644522 None:intergenic 20.0%
!! GGTAATAAAATAAAACTAAG+AGG - chr8_3:37644735-37644754 Msa1287360:CDS 20.0%
!!! ATGTAAGTTTTAAAACTAGA+GGG + chr8_3:37645148-37645167 None:intergenic 20.0%
!!! AATGTAAGTTTTAAAACTAG+AGG + chr8_3:37645149-37645168 None:intergenic 20.0%
!! TAGATTAAAGATAAAGTGAT+GGG + chr8_3:37644470-37644489 None:intergenic 20.0%
!!! ATAGGCTACTTTTTTTTTAT+TGG + chr8_3:37644503-37644522 None:intergenic 20.0%
!! GGTAATAAAATAAAACTAAG+AGG - chr8_3:37644735-37644754 Msa1287360:CDS 20.0%
!!! ATGTAAGTTTTAAAACTAGA+GGG + chr8_3:37645148-37645167 None:intergenic 20.0%
!!! AATGTAAGTTTTAAAACTAG+AGG + chr8_3:37645149-37645168 None:intergenic 20.0%
! AGATTAAAGATAAAGTGATG+GGG + chr8_3:37644469-37644488 None:intergenic 25.0%
! GTAGATTAAAGATAAAGTGA+TGG + chr8_3:37644471-37644490 None:intergenic 25.0%
! AGTTACATCATAGAAATCTA+GGG + chr8_3:37644683-37644702 None:intergenic 25.0%
! TATAAATCGAGCTATATCAA+TGG + chr8_3:37644710-37644729 None:intergenic 25.0%
! TATAGCTCGATTTATAAAGA+AGG - chr8_3:37644714-37644733 Msa1287360:CDS 25.0%
! AAACTAAGAGGATTGTTTAT+TGG - chr8_3:37644747-37644766 Msa1287360:CDS 25.0%
! ATGTGTTAGTTAATTATTGC+AGG - chr8_3:37644807-37644826 Msa1287360:intron 25.0%
! AATTATTGCAGGAATTTGAT+AGG - chr8_3:37644818-37644837 Msa1287360:intron 25.0%
! ATTATTGCAGGAATTTGATA+GGG - chr8_3:37644819-37644838 Msa1287360:intron 25.0%
!!! TGCTTCATCTATTTTTGTAT+AGG - chr8_3:37644910-37644929 Msa1287360:CDS 25.0%
!!! CTATTTTTGTATAGGTAGTA+TGG - chr8_3:37644918-37644937 Msa1287360:CDS 25.0%
!!! TGTAAGTTTTAAAACTAGAG+GGG + chr8_3:37645147-37645166 None:intergenic 25.0%
! AGATTAAAGATAAAGTGATG+GGG + chr8_3:37644469-37644488 None:intergenic 25.0%
! GTAGATTAAAGATAAAGTGA+TGG + chr8_3:37644471-37644490 None:intergenic 25.0%
! AGTTACATCATAGAAATCTA+GGG + chr8_3:37644683-37644702 None:intergenic 25.0%
! TATAAATCGAGCTATATCAA+TGG + chr8_3:37644710-37644729 None:intergenic 25.0%
! TATAGCTCGATTTATAAAGA+AGG - chr8_3:37644714-37644733 Msa1287360:CDS 25.0%
! AAACTAAGAGGATTGTTTAT+TGG - chr8_3:37644747-37644766 Msa1287360:CDS 25.0%
! ATGTGTTAGTTAATTATTGC+AGG - chr8_3:37644807-37644826 Msa1287360:intron 25.0%
! AATTATTGCAGGAATTTGAT+AGG - chr8_3:37644818-37644837 Msa1287360:intron 25.0%
! ATTATTGCAGGAATTTGATA+GGG - chr8_3:37644819-37644838 Msa1287360:intron 25.0%
!!! TGCTTCATCTATTTTTGTAT+AGG - chr8_3:37644910-37644929 Msa1287360:CDS 25.0%
!!! CTATTTTTGTATAGGTAGTA+TGG - chr8_3:37644918-37644937 Msa1287360:CDS 25.0%
!!! TGTAAGTTTTAAAACTAGAG+GGG + chr8_3:37645147-37645166 None:intergenic 25.0%
GATTAAAGATAAAGTGATGG+GGG + chr8_3:37644468-37644487 None:intergenic 30.0%
CAGTTACATCATAGAAATCT+AGG + chr8_3:37644684-37644703 None:intergenic 30.0%
AGATTTCTATGATGTAACTG+AGG - chr8_3:37644684-37644703 Msa1287360:CDS 30.0%
! TATGGCAATTTTGCTCTTTA+AGG - chr8_3:37644936-37644955 Msa1287360:CDS 30.0%
! ATGGCAATTTTGCTCTTTAA+GGG - chr8_3:37644937-37644956 Msa1287360:CDS 30.0%
TGAAAAACAAAATATGGCAG+AGG - chr8_3:37645101-37645120 Msa1287360:five_prime_UTR 30.0%
!!! GTAAGTTTTAAAACTAGAGG+GGG + chr8_3:37645146-37645165 None:intergenic 30.0%
GATTAAAGATAAAGTGATGG+GGG + chr8_3:37644468-37644487 None:intergenic 30.0%
CAGTTACATCATAGAAATCT+AGG + chr8_3:37644684-37644703 None:intergenic 30.0%
AGATTTCTATGATGTAACTG+AGG - chr8_3:37644684-37644703 Msa1287360:CDS 30.0%
! TATGGCAATTTTGCTCTTTA+AGG - chr8_3:37644936-37644955 Msa1287360:CDS 30.0%
! ATGGCAATTTTGCTCTTTAA+GGG - chr8_3:37644937-37644956 Msa1287360:CDS 30.0%
TGAAAAACAAAATATGGCAG+AGG - chr8_3:37645101-37645120 Msa1287360:five_prime_UTR 30.0%
!!! GTAAGTTTTAAAACTAGAGG+GGG + chr8_3:37645146-37645165 None:intergenic 30.0%
GCACAAGAGTAGAACAAAAT+AGG + chr8_3:37644521-37644540 None:intergenic 35.0%
CTTCAATTCATCATGCTAGA+AGG - chr8_3:37644586-37644605 Msa1287360:exon 35.0%
! ATCATAGAAATCTAGGGCTT+TGG + chr8_3:37644677-37644696 None:intergenic 35.0%
AAATCAGTTCTCAGCAATAG+AGG - chr8_3:37644984-37645003 Msa1287360:CDS 35.0%
GCACAAGAGTAGAACAAAAT+AGG + chr8_3:37644521-37644540 None:intergenic 35.0%
CTTCAATTCATCATGCTAGA+AGG - chr8_3:37644586-37644605 Msa1287360:exon 35.0%
! ATCATAGAAATCTAGGGCTT+TGG + chr8_3:37644677-37644696 None:intergenic 35.0%
AAATCAGTTCTCAGCAATAG+AGG - chr8_3:37644984-37645003 Msa1287360:CDS 35.0%
ATTCATCATGCTAGAAGGCA+AGG - chr8_3:37644591-37644610 Msa1287360:exon 40.0%
CAGGAATTTGATAGGGTTCA+TGG - chr8_3:37644826-37644845 Msa1287360:intron 40.0%
!!! TGCATTGTAGGCACTGATTT+TGG - chr8_3:37644862-37644881 Msa1287360:intron 40.0%
TCCTTTGTGACACATTGTAG+TGG - chr8_3:37644886-37644905 Msa1287360:CDS 40.0%
!! AACTGATTTTCCTCTGCTTG+TGG + chr8_3:37644973-37644992 None:intergenic 40.0%
! TTTAAAACTAGAGGGGGTGT+TGG + chr8_3:37645140-37645159 None:intergenic 40.0%
ATTCATCATGCTAGAAGGCA+AGG - chr8_3:37644591-37644610 Msa1287360:exon 40.0%
CAGGAATTTGATAGGGTTCA+TGG - chr8_3:37644826-37644845 Msa1287360:intron 40.0%
!!! TGCATTGTAGGCACTGATTT+TGG - chr8_3:37644862-37644881 Msa1287360:intron 40.0%
TCCTTTGTGACACATTGTAG+TGG - chr8_3:37644886-37644905 Msa1287360:CDS 40.0%
!! AACTGATTTTCCTCTGCTTG+TGG + chr8_3:37644973-37644992 None:intergenic 40.0%
! TTTAAAACTAGAGGGGGTGT+TGG + chr8_3:37645140-37645159 None:intergenic 40.0%
! GATAAAGTGATGGGGGATGA+TGG + chr8_3:37644461-37644480 None:intergenic 45.0%
TTTGATAGGGTTCATGGACC+TGG - chr8_3:37644832-37644851 Msa1287360:intron 45.0%
GCCACTACAATGTGTCACAA+AGG + chr8_3:37644890-37644909 None:intergenic 45.0%
! TTGCTCTTTAAGGGATCTGC+TGG - chr8_3:37644946-37644965 Msa1287360:CDS 45.0%
! GATAAAGTGATGGGGGATGA+TGG + chr8_3:37644461-37644480 None:intergenic 45.0%
TTTGATAGGGTTCATGGACC+TGG - chr8_3:37644832-37644851 Msa1287360:intron 45.0%
GCCACTACAATGTGTCACAA+AGG + chr8_3:37644890-37644909 None:intergenic 45.0%
! TTGCTCTTTAAGGGATCTGC+TGG - chr8_3:37644946-37644965 Msa1287360:CDS 45.0%
! AAAGTGATGGGGGATGATGG+TGG + chr8_3:37644458-37644477 None:intergenic 50.0%
CTAGAAGGCAAGGCAGTGAT+TGG - chr8_3:37644601-37644620 Msa1287360:CDS 50.0%
ATAGGGTTCATGGACCTGGT+TGG - chr8_3:37644836-37644855 Msa1287360:intron 50.0%
CCTACAATGCATTGCCAACC+AGG + chr8_3:37644853-37644872 None:intergenic 50.0%
CCTGGTTGGCAATGCATTGT+AGG - chr8_3:37644850-37644869 Msa1287360:intron 50.0%
! AAAGTGATGGGGGATGATGG+TGG + chr8_3:37644458-37644477 None:intergenic 50.0%
CTAGAAGGCAAGGCAGTGAT+TGG - chr8_3:37644601-37644620 Msa1287360:CDS 50.0%
ATAGGGTTCATGGACCTGGT+TGG - chr8_3:37644836-37644855 Msa1287360:intron 50.0%
CCTACAATGCATTGCCAACC+AGG + chr8_3:37644853-37644872 None:intergenic 50.0%
CCTGGTTGGCAATGCATTGT+AGG - chr8_3:37644850-37644869 Msa1287360:intron 50.0%
ATCTGCTGGTCCACAAGCAG+AGG - chr8_3:37644960-37644979 Msa1287360:CDS 55.0%
ATCTGCTGGTCCACAAGCAG+AGG - chr8_3:37644960-37644979 Msa1287360:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 37644428 37645187 37644428 ID=Msa1287360;Name=Msa1287360
chr8_3 mRNA 37644428 37645187 37644428 ID=Msa1287360-mRNA-1;Parent=Msa1287360;Name=Msa1287360-mRNA-1;_AED=0.06;_eAED=0.06;_QI=170|1|1|1|1|1|2|167|109
chr8_3 exon 37644428 37644786 37644428 ID=Msa1287360-mRNA-1:exon:10791;Parent=Msa1287360-mRNA-1
chr8_3 exon 37644880 37645187 37644880 ID=Msa1287360-mRNA-1:exon:10790;Parent=Msa1287360-mRNA-1
chr8_3 five_prime_UTR 37645018 37645187 37645018 ID=Msa1287360-mRNA-1:five_prime_utr;Parent=Msa1287360-mRNA-1
chr8_3 CDS 37644880 37645017 37644880 ID=Msa1287360-mRNA-1:cds;Parent=Msa1287360-mRNA-1
chr8_3 CDS 37644595 37644786 37644595 ID=Msa1287360-mRNA-1:cds;Parent=Msa1287360-mRNA-1
chr8_3 three_prime_UTR 37644428 37644594 37644428 ID=Msa1287360-mRNA-1:three_prime_utr;Parent=Msa1287360-mRNA-1
Gene Sequence

>Msa1287360

ATGCTAGAAGGCAAGGCAGTGATTGGTGAGACTGACATGCTTCATACTATGCAACAAGATGCTCTTGATTTAGCATCCAAAGCCCTAGATTTCTATGATGTAACTGAGGCCATTGATATAGCTCGATTTATAAAGAAGGAATTTGATAGGGTTCATGGACCTGGTTGGCAATGCATTGTAGGCACTGATTTTGGTTCCTTTGTGACACATTGTAGTGGCTGCTTCATCTATTTTTGTATAGGTAGTATGGCAATTTTGCTCTTTAAGGGATCTGCTGGTCCACAAGCAGAGGAAAATCAGTTCTCAGCAATAGAGGCAACTAAAGCATAA

Protein sequence

>Msa1287360

MLEGKAVIGETDMLHTMQQDALDLASKALDFYDVTEAIDIARFIKKEFDRVHGPGWQCIVGTDFGSFVTHCSGCFIYFCIGSMAILLFKGSAGPQAEENQFSAIEATKA*