AlfalfaGEDB | Msa1112630

M. sativa cultivar zhongmu-4 / Msa1112630

KEGG: -
GO: -
PFAM: PF00228.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1112630	AES80148.2	88.991	109	10	2	1	109	1	107	2.87e-50	164

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1112630	sp\|Q8W4Y8\|IBB_LENCU	50.476	105	46	2	5	107	10	110	1.83e-30	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1112630	G7L3M8	88.991	109	10	2	1	109	1	107	1.37e-50	164

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0064990	Msa1112630	0.818593	1.807668e-52	-8.615850e-47
Msa0689840	Msa1112630	0.806341	8.621686e-50	-8.615850e-47
Msa0728210	Msa1112630	0.810472	1.133449e-50	-8.615850e-47
Msa0744550	Msa1112630	0.804276	2.335195e-49	-8.615850e-47
Msa0789410	Msa1112630	0.817495	3.201904e-52	-8.615850e-47
Msa0814350	Msa1112630	0.807926	3.981213e-50	-8.615850e-47
Msa0841670	Msa1112630	0.800615	1.326618e-48	-8.615850e-47
Msa0183960	Msa1112630	-0.801313	9.552641e-49	-8.615850e-47
Msa0370620	Msa1112630	0.803101	4.094285e-49	-8.615850e-47
Msa0374040	Msa1112630	0.803008	4.278085e-49	-8.615850e-47
Msa0409490	Msa1112630	0.806339	8.629396e-50	-8.615850e-47
Msa0414480	Msa1112630	0.804436	2.161801e-49	-8.615850e-47
Msa0480540	Msa1112630	0.801769	7.702381e-49	-8.615850e-47
Msa0996980	Msa1112630	0.813056	3.104251e-51	-8.615850e-47
Msa1037530	Msa1112630	0.809813	1.571952e-50	-8.615850e-47
Msa0248320	Msa1112630	0.818906	1.534620e-52	-8.615850e-47
Msa0303900	Msa1112630	0.813896	2.029712e-51	-8.615850e-47
Msa0333770	Msa1112630	0.809045	2.296739e-50	-8.615850e-47
Msa0354550	Msa1112630	0.825971	3.502926e-54	-8.615850e-47
Msa0361700	Msa1112630	0.800362	1.493453e-48	-8.615850e-47
Msa1112630	Msa1130410	0.805870	1.083274e-49	-8.615850e-47
Msa1112630	Msa1138110	0.803917	2.773067e-49	-8.615850e-47
Msa1112630	Msa1173290	0.800293	1.542420e-48	-8.615850e-47
Msa1112630	Msa1214960	0.809491	1.843109e-50	-8.615850e-47
Msa1112630	Msa1375640	0.805058	1.602968e-49	-8.615850e-47
Msa1112630	Msa1376520	0.808117	3.625900e-50	-8.615850e-47
Msa1112630	Msa1419390	0.814812	1.273478e-51	-8.615850e-47
Msa1112630	Msa1419700	0.804267	2.345092e-49	-8.615850e-47
Msa1112630	Msa1429190	0.803155	3.989657e-49	-8.615850e-47
Msa1112630	Msa1446090	0.803962	2.713873e-49	-8.615850e-47
Msa0496070	Msa1112630	0.817273	3.591153e-52	-8.615850e-47
Msa0538650	Msa1112630	0.808671	2.762315e-50	-8.615850e-47
Msa0574220	Msa1112630	-0.803974	2.698638e-49	-8.615850e-47
Msa0594470	Msa1112630	0.806754	7.053286e-50	-8.615850e-47
Msa0896920	Msa1112630	0.814289	1.662400e-51	-8.615850e-47
Msa0902340	Msa1112630	0.807653	4.550153e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1112630	MtrunA17_Chr7g0247451	87.156	109	12	2	1	109	1	107	5.57e-54	164
Msa1112630	MtrunA17_Chr7g0247571	54.464	112	45	2	3	109	48	158	1.38e-37	124
Msa1112630	MtrunA17_Chr7g0247471	58.163	98	37	2	7	101	14	110	4.31e-33	111
Msa1112630	MtrunA17_Chr7g0247531	55.769	104	38	2	5	101	1	103	9.61e-33	110
Msa1112630	MtrunA17_Chr7g0247461	54.128	109	37	4	3	101	27	132	1.99e-31	107
Msa1112630	MtrunA17_Chr7g0247521	56.190	105	39	4	1	101	1	102	9.06e-29	100
Msa1112630	MtrunA17_Chr7g0247481	53.000	100	40	3	5	98	2	100	1.25e-28	99.8
Msa1112630	MtrunA17_Chr7g0247511	43.269	104	51	3	5	101	11	113	3.52e-21	81.3
Msa1112630	MtrunA17_Chr7g0247541	40.625	96	52	3	5	98	10	102	3.10e-14	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAAAACGAGCATCAATAA+TGG	0.364997	7_3:+58594033	None:intergenic
ACTCAGCTTGCAAGACTTGT+AGG	0.371610	7_3:-58593877	Msa1112630:CDS
ACTTGAGTGATGAGGGAGTT+TGG	0.371670	7_3:+58594010	None:intergenic
TGTTGTCGATTTGACACCAT+TGG	0.451422	7_3:+58593982	None:intergenic
TCATTAATCTTAATGAGCCT+TGG	0.460999	7_3:+58593772	None:intergenic
AGCAAAAGTCAGTGATATCA+AGG	0.473824	7_3:+58593822	None:intergenic
GTTATAGTAGTTGAGTTACA+TGG	0.521465	7_3:+58593794	None:intergenic
ACGAGCATCAATAATGGTTG+TGG	0.542447	7_3:+58594039	None:intergenic
TCAAGGCAATCGCACTTCAA+AGG	0.562557	7_3:+58593839	None:intergenic
GGATATCACTTGAGTGATGA+GGG	0.569030	7_3:+58594003	None:intergenic
TAACTCAACTACTATAACCA+AGG	0.583203	7_3:-58593789	Msa1112630:CDS
ATCACTCAAGTGATATCCAA+TGG	0.590793	7_3:-58593998	Msa1112630:CDS
GCACCTTGTTGTGATAATTG+TGG	0.595238	7_3:-58593956	Msa1112630:CDS
TTGCAAGCTGAGTGACATGT+TGG	0.596983	7_3:+58593887	None:intergenic
TGGATATCACTTGAGTGATG+AGG	0.599239	7_3:+58594002	None:intergenic
TCAGTACAACGACACTGAGG+AGG	0.627030	7_3:+58593917	None:intergenic
CAGTACAACGACACTGAGGA+GGG	0.637399	7_3:+58593918	None:intergenic
CAACCACAATTATCACAACA+AGG	0.653733	7_3:+58593953	None:intergenic
ACATCAGTACAACGACACTG+AGG	0.777519	7_3:+58593914	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GCTTTGTTACTTTTTTTCAT+AGG	-	chr7_3:58593802-58593821	Msa1112630:CDS	25.0%
	TTCAAAACGAGCATCAATAA+TGG	+	chr7_3:58593839-58593858	None:intergenic	30.0%
	GTTATAGTAGTTGAGTTACA+TGG	+	chr7_3:58594078-58594097	None:intergenic	30.0%
	TAACTCAACTACTATAACCA+AGG	-	chr7_3:58594080-58594099	Msa1112630:CDS	30.0%
	ATCACTCAAGTGATATCCAA+TGG	-	chr7_3:58593871-58593890	Msa1112630:CDS	35.0%
	CAACCACAATTATCACAACA+AGG	+	chr7_3:58593919-58593938	None:intergenic	35.0%
	AGCAAAAGTCAGTGATATCA+AGG	+	chr7_3:58594050-58594069	None:intergenic	35.0%
	ACGAGCATCAATAATGGTTG+TGG	+	chr7_3:58593833-58593852	None:intergenic	40.0%
	GGATATCACTTGAGTGATGA+GGG	+	chr7_3:58593869-58593888	None:intergenic	40.0%
	TGGATATCACTTGAGTGATG+AGG	+	chr7_3:58593870-58593889	None:intergenic	40.0%
!!	TGTTGTCGATTTGACACCAT+TGG	+	chr7_3:58593890-58593909	None:intergenic	40.0%
!	GCACCTTGTTGTGATAATTG+TGG	-	chr7_3:58593913-58593932	Msa1112630:CDS	40.0%
	ACTTGAGTGATGAGGGAGTT+TGG	+	chr7_3:58593862-58593881	None:intergenic	45.0%
	ACATCAGTACAACGACACTG+AGG	+	chr7_3:58593958-58593977	None:intergenic	45.0%
	TTGCAAGCTGAGTGACATGT+TGG	+	chr7_3:58593985-58594004	None:intergenic	45.0%
	ACTCAGCTTGCAAGACTTGT+AGG	-	chr7_3:58593992-58594011	Msa1112630:CDS	45.0%
	TCAAGGCAATCGCACTTCAA+AGG	+	chr7_3:58594033-58594052	None:intergenic	45.0%
	CAGTACAACGACACTGAGGA+GGG	+	chr7_3:58593954-58593973	None:intergenic	50.0%
	TCAGTACAACGACACTGAGG+AGG	+	chr7_3:58593955-58593974	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	58593781	58594110	58593781	ID=Msa1112630;Name=Msa1112630
chr7_3	mRNA	58593781	58594110	58593781	ID=Msa1112630-mRNA-1;Parent=Msa1112630;Name=Msa1112630-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|109
chr7_3	exon	58593781	58594110	58593781	ID=Msa1112630-mRNA-1:exon:12919;Parent=Msa1112630-mRNA-1
chr7_3	CDS	58593781	58594110	58593781	ID=Msa1112630-mRNA-1:cds;Parent=Msa1112630-mRNA-1

Gene Sequence	>Msa1112630 ATGGAGTTGAAGATGAAGATAGCTTTGTTACTTTTTTTCATAGGTTTGACCACAACCATTATTGATGCTCGTTTTGAACCAAACTCCCTCATCACTCAAGTGATATCCAATGGTGTCAAATCGACAACATGTGCACCTTGTTGTGATAATTGTGGTTGCACAAAATCAATCCCTCCTCAGTGTCGTTGTACTGATGTTAAACCAACATGTCACTCAGCTTGCAAGACTTGTAGGTGTTTTGAGACATTACCTTTGAAGTGCGATTGCCTTGATATCACTGACTTTTGCTATGAACCATGTAACTCAACTACTATAACCAAGGCTCATTAA
Protein sequence	>Msa1112630 MELKMKIALLLFFIGLTTTIIDARFEPNSLITQVISNGVKSTTCAPCCDNCGCTKSIPPQCRCTDVKPTCHSACKTCRCFETLPLKCDCLDITDFCYEPCNSTTITKAH*