AlfalfaGEDB | Msa0538650

M. sativa cultivar zhongmu-4 / Msa0538650

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0538650	RHN59446.1	58.400	125	45	3	1	125	1	118	2.98e-23	98.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0538650	A0A396I1F2	58.400	125	45	3	1	125	1	118	1.42e-23	98.6

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012790	Msa0538650	0.805686	1.184194e-49	-8.615850e-47
Msa0012810	Msa0538650	0.812243	4.676999e-51	-8.615850e-47
Msa0021400	Msa0538650	0.805927	1.053863e-49	-8.615850e-47
Msa0024650	Msa0538650	0.804275	2.335546e-49	-8.615850e-47
Msa0034100	Msa0538650	-0.833821	4.278905e-56	-8.615850e-47
Msa0036550	Msa0538650	0.831869	1.307120e-55	-8.615850e-47
Msa0036560	Msa0538650	-0.813701	2.240184e-51	-8.615850e-47
Msa0036580	Msa0538650	0.814627	1.399310e-51	-8.615850e-47
Msa0038010	Msa0538650	0.830930	2.225140e-55	-8.615850e-47
Msa0046430	Msa0538650	0.821669	3.571555e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0538650	MtrunA17_Chr4g0013381	58.400	125	45	3	1	125	1	118	2.74e-27	98.6
Msa0538650	MtrunA17_Chr4g0013451	49.367	158	60	4	6	144	18	174	1.15e-19	84.3
Msa0538650	MtrunA17_Chr4g0013451	50.350	143	51	5	21	144	3	144	1.03e-18	81.6
Msa0538650	MtrunA17_Chr4g0013451	44.509	173	61	5	6	144	58	229	4.22e-17	77.0
Msa0538650	MtrunA17_Chr4g0013451	46.061	165	57	6	5	144	92	249	1.71e-16	75.1
Msa0538650	MtrunA17_Chr4g0013451	45.912	159	57	5	10	144	192	345	2.40e-16	74.7
Msa0538650	MtrunA17_Chr4g0013451	46.108	167	56	6	7	144	214	375	1.39e-15	72.8
Msa0538650	MtrunA17_Chr4g0013451	39.286	168	70	5	10	152	308	468	4.16e-14	68.6
Msa0538650	MtrunA17_Chr4g0013421	48.750	160	62	4	6	146	18	176	1.70e-19	84.0
Msa0538650	MtrunA17_Chr4g0013421	51.049	143	50	5	21	144	3	144	5.07e-19	82.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 74 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCGGAGGCTTGTCATAAT+TGG	0.298691	4_1:+18126991	Msa0538650:CDS
AAGTTGTACCATCTACCCGA+GGG	0.700819	4_1:+18126327	Msa0538650:five_prime_UTR
ACAGGAGGCTTCTCAACGGG+TGG	0.644775	4_1:+18126632	Msa0538650:CDS
ACAGGAGGCTTCTCAACTGG+TGG	0.640818	4_1:+18126797	Msa0538650:CDS
ACAGGTGGCTTGTAAACCGG+AGG	0.689338	4_1:+18126767	Msa0538650:intron
ACAGGTGGCTTGTACACCGG+TGG	0.693604	4_1:+18126572	Msa0538650:intron
ACAGGTGGCTTGTAGACTGG+AGG	0.678345	4_1:+18126707	Msa0538650:CDS
ACAGGTGGCTTGTAGACTGG+AGG	0.680593	4_1:+18126662	Msa0538650:CDS
ACCGGAGGCTTGTAAACAGG+AGG	0.635764	4_1:+18126827	Msa0538650:CDS
ACCGGAGGCTTGTAAACAGG+AGG	0.636882	4_1:+18126782	Msa0538650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGACTGGAGGTTTGTATAC+AGG	+	chr4_1:18126749-18126768	Msa0538650:intron	40.0%
TAAACAGGAGGTTTGTAGAC+CGG	+	chr4_1:18126839-18126858	Msa0538650:intron	40.0%
AAGTTGTACCATCTACCCGA+GGG	+	chr4_1:18126327-18126346	Msa0538650:five_prime_UTR	45.0%
TAGACTGGAGGTTTCTCAAC+TGG	+	chr4_1:18126419-18126438	Msa0538650:CDS	45.0%
AGACTGGAGGTTTCTCAACT+GGG	+	chr4_1:18126420-18126439	Msa0538650:CDS	45.0%
CTCAACTGGAGGCTTGTAAA+CGG	+	chr4_1:18126523-18126542	Msa0538650:intron	45.0%
TCAACTGGAGGCTTGTAAAC+GGG	+	chr4_1:18126524-18126543	Msa0538650:intron	45.0%
TAGACTGGAGGCTTGTATAC+AGG	+	chr4_1:18126554-18126573	Msa0538650:intron	45.0%
TAAACTGGAGGCTTGTACAC+CGG	+	chr4_1:18126599-18126618	Msa0538650:CDS	45.0%
GTAAACAGGAGGCTTCTCAA+CGG	+	chr4_1:18126628-18126647	Msa0538650:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	18126313	18127058	18126313	ID=Msa0538650;Name=Msa0538650
chr4_1	mRNA	18126313	18127058	18126313	ID=Msa0538650-mRNA-1;Parent=Msa0538650;Name=Msa0538650-mRNA-1;_AED=0.05;_eAED=0.38;_QI=41\|0\|0.5\|1\|0\|0\|4\|27\|152
chr4_1	exon	18126313	18126455	18126313	ID=Msa0538650-mRNA-1:exon:5358;Parent=Msa0538650-mRNA-1
chr4_1	exon	18126576	18126758	18126576	ID=Msa0538650-mRNA-1:exon:5359;Parent=Msa0538650-mRNA-1
chr4_1	exon	18126771	18126848	18126771	ID=Msa0538650-mRNA-1:exon:5360;Parent=Msa0538650-mRNA-1
chr4_1	exon	18126936	18127058	18126936	ID=Msa0538650-mRNA-1:exon:5361;Parent=Msa0538650-mRNA-1
chr4_1	five_prime_UTR	18126313	18126353	18126313	ID=Msa0538650-mRNA-1:five_prime_utr;Parent=Msa0538650-mRNA-1
chr4_1	CDS	18126354	18126455	18126354	ID=Msa0538650-mRNA-1:cds;Parent=Msa0538650-mRNA-1
chr4_1	CDS	18126576	18126758	18126576	ID=Msa0538650-mRNA-1:cds;Parent=Msa0538650-mRNA-1
chr4_1	CDS	18126771	18126848	18126771	ID=Msa0538650-mRNA-1:cds;Parent=Msa0538650-mRNA-1
chr4_1	CDS	18126936	18127031	18126936	ID=Msa0538650-mRNA-1:cds;Parent=Msa0538650-mRNA-1
chr4_1	three_prime_UTR	18127032	18127058	18127032	ID=Msa0538650-mRNA-1:three_prime_utr;Parent=Msa0538650-mRNA-1

Gene Sequence

>Msa0538650

ATGGGTGGTGAGGAGGTCCGTAAACTGGTGGCTTCTCGACTGGAGGCTTGTAAACTGGTGGCTTGTAGACTGGAGGTTTCTCAACTGGGGGCTTGTAAACAGGTGGCTTGTACACCGGTGGCTTGTAAACTGGAGGCTTGTACACCGGAGGCTTGTAAACAGGAGGCTTCTCAACGGGTGGCTTGTAAACAGGTGGCTTGTAGACTGGAGGCTTGTAGACTGGAGGCTTGTATACAGGTGGCTTGTAGACTGGAGGCTTCTCAACTGGTGGCTTGTAGACTGGAGGTGGCTTGTAAACCGGAGGCTTGTAAACAGGAGGCTTCTCAACTGGTGGCTTGTACACCGGAGGCTTGTAAACAGGAGGGGGTTTGTAGACAGGGGGTTTCTCAACTGGTGGCTTATACACCGGTGGTTGGTAAACCGGAGGCTTGTCATAATTGGCAAGACCCTGAGGGATGA

Protein sequence

>Msa0538650

MGGEEVRKLVASRLEACKLVACRLEVSQLGACKQVACTPVACKLEACTPEACKQEASQRVACKQVACRLEACRLEACIQVACRLEASQLVACRLEVACKPEACKQEASQLVACTPEACKQEGVCRQGVSQLVAYTPVVGKPEACHNWQDPEG*