AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0409490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0409490 MtrunA17_Chr3g0101331 70.103 97 28 1 20 115 412 508 4.32e-38 135
Msa0409490 MtrunA17_Chr3g0112511 65.979 97 32 1 20 115 395 491 2.10e-36 130
Msa0409490 MtrunA17_Chr3g0100831 66.667 96 31 1 21 115 400 495 2.36e-36 130
Msa0409490 MtrunA17_Chr1g0189321 64.948 97 27 2 23 115 1 94 5.44e-36 123
Msa0409490 MtrunA17_Chr3g0100731 64.646 99 28 2 21 115 160 255 1.82e-35 127
Msa0409490 MtrunA17_Chr3g0100681 65.625 96 32 1 21 115 218 313 4.50e-35 126
Msa0409490 MtrunA17_Chr3g0100971 64.583 96 33 1 21 115 402 497 1.94e-34 125
Msa0409490 MtrunA17_Chr3g0100961 63.542 96 34 1 21 115 405 500 2.56e-34 124
Msa0409490 MtrunA17_Chr3g0101391 65.625 96 32 1 21 115 87 182 8.77e-34 122
Msa0409490 MtrunA17_Chr3g0100851 60.417 96 37 1 21 115 281 376 2.04e-33 122
Msa0409490 MtrunA17_Chr3g0100761 62.500 96 35 1 21 115 362 457 6.64e-33 120
Msa0409490 MtrunA17_Chr1g0189351 62.887 97 35 1 20 115 401 497 5.07e-32 118
Msa0409490 MtrunA17_Chr3g0100801 82.812 64 11 0 52 115 393 456 2.80e-31 115
Msa0409490 MtrunA17_Chr3g0101321 61.458 96 36 1 21 115 397 492 4.00e-31 115
Msa0409490 MtrunA17_Chr1g0189271 64.286 84 29 1 20 102 241 324 3.63e-29 107
Msa0409490 MtrunA17_Chr1g0149071 61.458 96 20 1 20 115 122 200 3.73e-29 108
Msa0409490 MtrunA17_Chr3g0101251 60.825 97 34 2 20 115 368 461 2.11e-28 107
Msa0409490 MtrunA17_Chr3g0101171 68.493 73 22 1 43 115 1 72 2.18e-28 99.8
Msa0409490 MtrunA17_Chr1g0151481 54.082 98 40 2 21 115 408 503 2.68e-28 107
Msa0409490 MtrunA17_Chr3g0080791 54.639 97 43 1 20 115 406 502 1.42e-27 105
Msa0409490 MtrunA17_Chr3g0080771 54.639 97 43 1 20 115 406 502 1.74e-27 105
Msa0409490 MtrunA17_Chr3g0094301 51.546 97 46 1 20 115 406 502 5.79e-26 100
Msa0409490 MtrunA17_Chr7g0229761 51.546 97 46 1 20 115 406 502 5.79e-26 100
Msa0409490 MtrunA17_Chr3g0081391 51.546 97 46 1 20 115 405 501 1.44e-25 99.8
Msa0409490 MtrunA17_Chr3g0080741 50.515 97 47 1 20 115 408 504 1.59e-25 99.8
Msa0409490 MtrunA17_Chr3g0101221 53.608 97 42 2 20 115 407 501 4.67e-25 98.2
Msa0409490 MtrunA17_Chr3g0101181 56.701 97 38 2 20 115 412 505 5.04e-25 98.2
Msa0409490 MtrunA17_Chr3g0101211 55.670 97 40 2 20 115 194 288 6.80e-25 97.1
Msa0409490 MtrunA17_Chr3g0080601 54.639 97 43 1 20 115 405 501 6.96e-25 97.8
Msa0409490 MtrunA17_Chr3g0081361 48.515 101 47 2 18 115 408 506 9.80e-25 97.4
Msa0409490 MtrunA17_Chr3g0080731 49.485 97 48 1 20 115 406 502 2.43e-24 96.3
Msa0409490 MtrunA17_Chr3g0084351 51.546 97 46 1 20 115 331 427 4.49e-24 95.5
Msa0409490 MtrunA17_Chr3g0101201 53.608 97 42 2 20 115 405 499 4.70e-24 95.5
Msa0409490 MtrunA17_Chr3g0101011 50.538 93 45 1 20 111 418 510 5.09e-24 95.5
Msa0409490 MtrunA17_Chr3g0080751 52.000 100 42 3 20 115 406 503 1.10e-23 94.4
Msa0409490 MtrunA17_Chr3g0080651 50.549 91 44 1 20 109 324 414 1.68e-23 94.0
Msa0409490 MtrunA17_Chr3g0080721 52.747 91 42 1 20 109 144 234 1.87e-23 93.6
Msa0409490 MtrunA17_Chr3g0081371 49.485 97 48 1 20 115 410 506 2.12e-23 93.6
Msa0409490 MtrunA17_Chr3g0081211 50.549 91 44 1 26 115 410 500 2.92e-23 93.2
Msa0409490 MtrunA17_Chr3g0081341 49.485 97 48 1 20 115 469 565 4.16e-23 92.8
Msa0409490 MtrunA17_Chr5g0444301 75.439 57 14 0 59 115 1 57 1.27e-22 90.9
Msa0409490 MtrunA17_Chr3g0081191 46.000 100 51 2 18 115 399 497 6.16e-22 89.4
Msa0409490 MtrunA17_Chr3g0101241 50.515 97 44 2 20 115 381 474 1.10e-20 85.9
Msa0409490 MtrunA17_Chr3g0101271 50.515 97 44 2 20 115 381 474 1.19e-20 85.9
Msa0409490 MtrunA17_Chr3g0100701 81.250 48 9 0 68 115 283 330 2.67e-20 84.7
Msa0409490 MtrunA17_Chr3g0101231 67.188 64 21 0 52 115 442 505 5.25e-20 84.0
Msa0409490 MtrunA17_Chr3g0081181 48.000 100 44 3 20 115 390 485 1.93e-19 82.4
Msa0409490 MtrunA17_Chr5g0410021 43.478 92 51 1 25 115 392 483 8.60e-17 74.7
Msa0409490 MtrunA17_Chr5g0410031 39.583 96 55 2 22 115 393 487 9.96e-17 74.7
Msa0409490 MtrunA17_Chr5g0409971 43.478 92 51 1 25 115 395 486 2.68e-16 73.2
Msa0409490 MtrunA17_Chr5g0410001 39.175 97 54 2 22 115 392 486 2.84e-16 73.2
Msa0409490 MtrunA17_Chr5g0409991 43.478 92 51 1 25 115 390 481 1.45e-15 71.2
Msa0409490 MtrunA17_Chr5g0404741 48.333 60 31 0 52 111 421 480 3.40e-14 67.4
Msa0409490 MtrunA17_Chr4g0037421 42.188 64 37 0 52 115 482 545 3.38e-13 64.3
Msa0409490 MtrunA17_Chr3g0100791 80.000 35 7 0 81 115 1 35 1.83e-12 62.4
Msa0409490 MtrunA17_Chr3g0080631 55.556 54 24 0 62 115 404 457 5.35e-12 61.2
Msa0409490 MtrunA17_Chr3g0101361 75.000 36 9 0 80 115 295 330 1.74e-11 59.7
Msa0409490 MtrunA17_Chr7g0219851 44.118 68 34 1 52 115 424 491 2.09e-11 59.3
Msa0409490 MtrunA17_Chr8g0344071 41.270 63 37 0 53 115 690 752 6.28e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCATGTTTGTTGTATTT+TGG 0.154976 3_2:-50093444 Msa0409490:CDS
GATTCAACTTCATAATCTTT+TGG 0.267002 3_2:+50093414 None:intergenic
ATGCATGATTTAAGATAAAA+AGG 0.281885 3_2:+50093460 None:intergenic
GTATCCTCACTTAGAATTGA+TGG 0.358881 3_2:-50093273 Msa0409490:CDS
CGAGCTGATTCATCGAAGTT+TGG 0.362846 3_2:-50093301 Msa0409490:CDS
AGATGAAAGGGGAGAAACTT+TGG 0.405413 3_2:-50093349 Msa0409490:CDS
AAGAAGAATTCCCATTTGAC+TGG 0.450935 3_2:-50093520 Msa0409490:CDS
TGATGATGAACTTAGCAAAC+TGG 0.482104 3_2:-50094299 None:intergenic
AGGATTTGTGAAAGATGAAA+GGG 0.497298 3_2:-50093361 Msa0409490:CDS
AAGGATTTGTGAAAGATGAA+AGG 0.537339 3_2:-50093362 Msa0409490:CDS
CAACGAGATCGATTCGACAA+TGG 0.573215 3_2:-50093392 Msa0409490:CDS
TCTATACTGTTTAGTCCTCA+CGG 0.582237 3_2:-50094271 Msa0409490:CDS
AGAAGAATTCCCATTTGACT+GGG 0.585440 3_2:-50093519 Msa0409490:CDS
ATTCGACAATGGATAGCTGA+AGG 0.603201 3_2:-50093381 Msa0409490:CDS
ACTTTGGAACAAGTTGCAGA+AGG 0.612209 3_2:-50093333 Msa0409490:CDS
TTATCCATCAATTCTAAGTG+AGG 0.617291 3_2:+50093269 None:intergenic
GGATTTGTGAAAGATGAAAG+GGG 0.653358 3_2:-50093360 Msa0409490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACACATTTATTTTTTATTA+AGG + chr3_2:50093718-50093737 None:intergenic 10.0%
!!! AACACATTTATTTTTTATTA+AGG + chr3_2:50093718-50093737 None:intergenic 10.0%
!!! TGTCTTTTATATTGATAATT+TGG - chr3_2:50093584-50093603 Msa0409490:intron 15.0%
!!! TGTCTTTTATATTGATAATT+TGG - chr3_2:50093584-50093603 Msa0409490:intron 15.0%
!! ATACAATTCAATATAAGGAT+TGG + chr3_2:50093550-50093569 None:intergenic 20.0%
!! CGATTATACAATTCAATATA+AGG + chr3_2:50093555-50093574 None:intergenic 20.0%
!!! ACTCAATTTTCTTGAATAAA+AGG + chr3_2:50093771-50093790 None:intergenic 20.0%
!! TACAAATTACAATCATTCAA+AGG + chr3_2:50093829-50093848 None:intergenic 20.0%
!!! TTTGGTTGTTATTTATACAA+CGG - chr3_2:50093930-50093949 Msa0409490:intron 20.0%
!!! ATGCATGATTTAAGATAAAA+AGG + chr3_2:50094106-50094125 None:intergenic 20.0%
!! ATACAATTCAATATAAGGAT+TGG + chr3_2:50093550-50093569 None:intergenic 20.0%
!! CGATTATACAATTCAATATA+AGG + chr3_2:50093555-50093574 None:intergenic 20.0%
!!! ACTCAATTTTCTTGAATAAA+AGG + chr3_2:50093771-50093790 None:intergenic 20.0%
!! TACAAATTACAATCATTCAA+AGG + chr3_2:50093829-50093848 None:intergenic 20.0%
!!! TTTGGTTGTTATTTATACAA+CGG - chr3_2:50093930-50093949 Msa0409490:intron 20.0%
!!! ATGCATGATTTAAGATAAAA+AGG + chr3_2:50094106-50094125 None:intergenic 20.0%
!!! TTTACACATCACTAGTTTTA+TGG - chr3_2:50093492-50093511 Msa0409490:intron 25.0%
!! CATGTAAATGCATTTTTACA+TGG + chr3_2:50093989-50094008 None:intergenic 25.0%
! CATGTAAAAATGCATTTACA+TGG - chr3_2:50093987-50094006 Msa0409490:intron 25.0%
!! GATTCAACTTCATAATCTTT+TGG + chr3_2:50094152-50094171 None:intergenic 25.0%
!!! TTTACACATCACTAGTTTTA+TGG - chr3_2:50093492-50093511 Msa0409490:intron 25.0%
!! CATGTAAATGCATTTTTACA+TGG + chr3_2:50093989-50094008 None:intergenic 25.0%
! CATGTAAAAATGCATTTACA+TGG - chr3_2:50093987-50094006 Msa0409490:intron 25.0%
!! GATTCAACTTCATAATCTTT+TGG + chr3_2:50094152-50094171 None:intergenic 25.0%
! TACATTGCTTCTTTCTTTTC+AGG - chr3_2:50093674-50093693 Msa0409490:intron 30.0%
TTAAGGAAATGTTCCAAAAC+AGG + chr3_2:50093701-50093720 None:intergenic 30.0%
CTTGAATAAAAGGACAAAGT+AGG + chr3_2:50093761-50093780 None:intergenic 30.0%
! TTCTTCTACTTCTGTAGTTT+TGG - chr3_2:50093912-50093931 Msa0409490:intron 30.0%
! TTTTCTTATCCCAGTCAAAT+GGG + chr3_2:50094056-50094075 None:intergenic 30.0%
! ACTGGGATAAGAAAAGTTTT+AGG - chr3_2:50094061-50094080 Msa0409490:intron 30.0%
GATAAAAAGGCAAATCATTG+TGG + chr3_2:50094093-50094112 None:intergenic 30.0%
!!! CATGCATGTTTGTTGTATTT+TGG - chr3_2:50094119-50094138 Msa0409490:intron 30.0%
AAGGATTTGTGAAAGATGAA+AGG - chr3_2:50094201-50094220 Msa0409490:intron 30.0%
AGGATTTGTGAAAGATGAAA+GGG - chr3_2:50094202-50094221 Msa0409490:intron 30.0%
TTATCCATCAATTCTAAGTG+AGG + chr3_2:50094297-50094316 None:intergenic 30.0%
! TACATTGCTTCTTTCTTTTC+AGG - chr3_2:50093674-50093693 Msa0409490:intron 30.0%
TTAAGGAAATGTTCCAAAAC+AGG + chr3_2:50093701-50093720 None:intergenic 30.0%
CTTGAATAAAAGGACAAAGT+AGG + chr3_2:50093761-50093780 None:intergenic 30.0%
! TTCTTCTACTTCTGTAGTTT+TGG - chr3_2:50093912-50093931 Msa0409490:intron 30.0%
! TTTTCTTATCCCAGTCAAAT+GGG + chr3_2:50094056-50094075 None:intergenic 30.0%
! ACTGGGATAAGAAAAGTTTT+AGG - chr3_2:50094061-50094080 Msa0409490:intron 30.0%
GATAAAAAGGCAAATCATTG+TGG + chr3_2:50094093-50094112 None:intergenic 30.0%
!!! CATGCATGTTTGTTGTATTT+TGG - chr3_2:50094119-50094138 Msa0409490:intron 30.0%
AAGGATTTGTGAAAGATGAA+AGG - chr3_2:50094201-50094220 Msa0409490:intron 30.0%
AGGATTTGTGAAAGATGAAA+GGG - chr3_2:50094202-50094221 Msa0409490:intron 30.0%
TTATCCATCAATTCTAAGTG+AGG + chr3_2:50094297-50094316 None:intergenic 30.0%
TCTATACTGTTTAGTCCTCA+CGG - chr3_2:50093292-50093311 Msa0409490:CDS 35.0%
!!! ACATCACTAGTTTTATGGCA+AGG - chr3_2:50093497-50093516 Msa0409490:intron 35.0%
GAGATTCACATTTCTTCAAG+TGG - chr3_2:50093640-50093659 Msa0409490:intron 35.0%
!!! TTTCTTTTCAGGTCCTGTTT+TGG - chr3_2:50093685-50093704 Msa0409490:intron 35.0%
TATATTACGAGAGCTTTGCT+TGG - chr3_2:50093861-50093880 Msa0409490:intron 35.0%
AAGAAGAATTCCCATTTGAC+TGG - chr3_2:50094043-50094062 Msa0409490:intron 35.0%
AGAAGAATTCCCATTTGACT+GGG - chr3_2:50094044-50094063 Msa0409490:intron 35.0%
! CTTTTCTTATCCCAGTCAAA+TGG + chr3_2:50094057-50094076 None:intergenic 35.0%
GGATTTGTGAAAGATGAAAG+GGG - chr3_2:50094203-50094222 Msa0409490:intron 35.0%
GTATCCTCACTTAGAATTGA+TGG - chr3_2:50094290-50094309 Msa0409490:CDS 35.0%
TCTATACTGTTTAGTCCTCA+CGG - chr3_2:50093292-50093311 Msa0409490:CDS 35.0%
!!! ACATCACTAGTTTTATGGCA+AGG - chr3_2:50093497-50093516 Msa0409490:intron 35.0%
GAGATTCACATTTCTTCAAG+TGG - chr3_2:50093640-50093659 Msa0409490:intron 35.0%
!!! TTTCTTTTCAGGTCCTGTTT+TGG - chr3_2:50093685-50093704 Msa0409490:intron 35.0%
TATATTACGAGAGCTTTGCT+TGG - chr3_2:50093861-50093880 Msa0409490:intron 35.0%
AAGAAGAATTCCCATTTGAC+TGG - chr3_2:50094043-50094062 Msa0409490:intron 35.0%
AGAAGAATTCCCATTTGACT+GGG - chr3_2:50094044-50094063 Msa0409490:intron 35.0%
! CTTTTCTTATCCCAGTCAAA+TGG + chr3_2:50094057-50094076 None:intergenic 35.0%
GGATTTGTGAAAGATGAAAG+GGG - chr3_2:50094203-50094222 Msa0409490:intron 35.0%
GTATCCTCACTTAGAATTGA+TGG - chr3_2:50094290-50094309 Msa0409490:CDS 35.0%
!! CAAGGTTCTAAACCCTCTAA+GGG - chr3_2:50093515-50093534 Msa0409490:CDS 40.0%
TGGAAAAACTAGCCCTTAGA+GGG + chr3_2:50093530-50093549 None:intergenic 40.0%
TTGGAAAAACTAGCCCTTAG+AGG + chr3_2:50093531-50093550 None:intergenic 40.0%
GATAATTTGGTCTGAACTGC+AGG - chr3_2:50093597-50093616 Msa0409490:intron 40.0%
ATTCGACAATGGATAGCTGA+AGG - chr3_2:50094182-50094201 Msa0409490:intron 40.0%
AGATGAAAGGGGAGAAACTT+TGG - chr3_2:50094214-50094233 Msa0409490:intron 40.0%
!! ACTTTGGAACAAGTTGCAGA+AGG - chr3_2:50094230-50094249 Msa0409490:intron 40.0%
!! CAAGGTTCTAAACCCTCTAA+GGG - chr3_2:50093515-50093534 Msa0409490:CDS 40.0%
TGGAAAAACTAGCCCTTAGA+GGG + chr3_2:50093530-50093549 None:intergenic 40.0%
TTGGAAAAACTAGCCCTTAG+AGG + chr3_2:50093531-50093550 None:intergenic 40.0%
GATAATTTGGTCTGAACTGC+AGG - chr3_2:50093597-50093616 Msa0409490:intron 40.0%
ATTCGACAATGGATAGCTGA+AGG - chr3_2:50094182-50094201 Msa0409490:intron 40.0%
AGATGAAAGGGGAGAAACTT+TGG - chr3_2:50094214-50094233 Msa0409490:intron 40.0%
!! ACTTTGGAACAAGTTGCAGA+AGG - chr3_2:50094230-50094249 Msa0409490:intron 40.0%
TCCTCACGGCTTGTCAAAAA+AGG - chr3_2:50093306-50093325 Msa0409490:CDS 45.0%
!!! ACCTTTTTTGACAAGCCGTG+AGG + chr3_2:50093310-50093329 None:intergenic 45.0%
!! GCAAGGTTCTAAACCCTCTA+AGG - chr3_2:50093514-50093533 Msa0409490:CDS 45.0%
!! CAACGAGATCGATTCGACAA+TGG - chr3_2:50094171-50094190 Msa0409490:intron 45.0%
! CGAGCTGATTCATCGAAGTT+TGG - chr3_2:50094262-50094281 Msa0409490:CDS 45.0%
TCCTCACGGCTTGTCAAAAA+AGG - chr3_2:50093306-50093325 Msa0409490:CDS 45.0%
!!! ACCTTTTTTGACAAGCCGTG+AGG + chr3_2:50093310-50093329 None:intergenic 45.0%
!! GCAAGGTTCTAAACCCTCTA+AGG - chr3_2:50093514-50093533 Msa0409490:CDS 45.0%
!! CAACGAGATCGATTCGACAA+TGG - chr3_2:50094171-50094190 Msa0409490:intron 45.0%
! CGAGCTGATTCATCGAAGTT+TGG - chr3_2:50094262-50094281 Msa0409490:CDS 45.0%
TTGGTCTGAACTGCAGGTGA+TGG - chr3_2:50093603-50093622 Msa0409490:intron 50.0%
! CTGCAGGTGATGGTCATCAA+TGG - chr3_2:50093613-50093632 Msa0409490:intron 50.0%
AGAGCTTTGCTTGGAGAGAG+AGG - chr3_2:50093870-50093889 Msa0409490:intron 50.0%
TTGGTCTGAACTGCAGGTGA+TGG - chr3_2:50093603-50093622 Msa0409490:intron 50.0%
! CTGCAGGTGATGGTCATCAA+TGG - chr3_2:50093613-50093632 Msa0409490:intron 50.0%
AGAGCTTTGCTTGGAGAGAG+AGG - chr3_2:50093870-50093889 Msa0409490:intron 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 50093269 50094316 50093269 ID=Msa0409490;Name=Msa0409490
chr3_2 mRNA 50093269 50094316 50093269 ID=Msa0409490-mRNA-1;Parent=Msa0409490;Name=Msa0409490-mRNA-1;_AED=0.44;_eAED=0.51;_QI=0|0|0|1|0|0|3|0|115
chr3_2 exon 50094258 50094316 50094258 ID=Msa0409490-mRNA-1:exon:9950;Parent=Msa0409490-mRNA-1
chr3_2 exon 50093510 50093564 50093510 ID=Msa0409490-mRNA-1:exon:9949;Parent=Msa0409490-mRNA-1
chr3_2 exon 50093269 50093502 50093269 ID=Msa0409490-mRNA-1:exon:9948;Parent=Msa0409490-mRNA-1
chr3_2 CDS 50094258 50094316 50094258 ID=Msa0409490-mRNA-1:cds;Parent=Msa0409490-mRNA-1
chr3_2 CDS 50093510 50093564 50093510 ID=Msa0409490-mRNA-1:cds;Parent=Msa0409490-mRNA-1
chr3_2 CDS 50093269 50093502 50093269 ID=Msa0409490-mRNA-1:cds;Parent=Msa0409490-mRNA-1
Gene Sequence

>Msa0409490

ATGAACTTAGCAAACTGGTGCAATCTATACTGTTTAGTCCTCACGGCTTGTCAAAAAAGTGAAAATATGACTTCAGAGCTAAAGAAGAATTCCCATTTGACTGGGATAAGAAAAGTTTTAGCCACAATGATTTGCCTTTTTATCTTAAATCATGCATGTTTGTTGTATTTTGGACTATATCCAAAAGATTATGAAGTTGAATCAACGAGATCGATTCGACAATGGATAGCTGAAGGATTTGTGAAAGATGAAAGGGGAGAAACTTTGGAACAAGTTGCAGAAGGATATTTAATCGAGCTGATTCATCGAAGTTTGGTACAAGTATCCTCACTTAGAATTGATGGATAA

Protein sequence

>Msa0409490

MNLANWCNLYCLVLTACQKSENMTSELKKNSHLTGIRKVLATMICLFILNHACLLYFGLYPKDYEVESTRSIRQWIAEGFVKDERGETLEQVAEGYLIELIHRSLVQVSSLRIDG*