AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999390 MtrunA17_Chr6g0485481 52.239 67 32 0 19 85 23 89 1.54e-19 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGAAGACATTGATTAT+TGG 0.204699 6_4:+81355738 Msa0999390:intron
AATCAAGTCCTTCATCGTTT+AGG 0.330565 6_4:-81355766 None:intergenic
GAGTTCCACCGGCTGCAATA+AGG 0.392268 6_4:-81355913 None:intergenic
GTTAATTCGATTATTAAAGC+CGG 0.475431 6_4:+81355829 Msa0999390:CDS
TTGGAAATTATCAAGAAGAA+TGG 0.513592 6_4:+81355882 Msa0999390:CDS
TTAATTCGATTATTAAAGCC+GGG 0.522204 6_4:+81355830 Msa0999390:CDS
CAAAGTTACCTTATTGCAGC+CGG 0.529219 6_4:+81355905 Msa0999390:CDS
ACCATGTAGAGGAGTTCAAT+CGG 0.544476 6_4:+81355132 Msa0999390:CDS
CTGTAAAAGTTAACATGTAG+AGG 0.559805 6_4:-81355935 None:intergenic
GCCGATTGAACTCCTCTACA+TGG 0.561743 6_4:-81355133 None:intergenic
TGGCAAGACCTAAACGATGA+AGG 0.570614 6_4:+81355758 Msa0999390:CDS
AGTTACCTTATTGCAGCCGG+TGG 0.602448 6_4:+81355908 Msa0999390:CDS
AACATGTAGAGGAGTTCCAC+CGG 0.612575 6_4:-81355924 None:intergenic
TTGTAAAAGTTACCATGTAG+AGG 0.612953 6_4:+81355121 Msa0999390:CDS
GAGTTCAATCGGCTGCAACA+AGG 0.644422 6_4:+81355143 Msa0999390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTTGAAAAAAAAATT+AGG + chr6_4:81355564-81355583 Msa0999390:intron 10.0%
!!! AATGTTTTGAAAAAAAAATT+AGG + chr6_4:81355564-81355583 Msa0999390:intron 10.0%
!!! TAAAAAATTAAAGTATTACC+CGG - chr6_4:81355851-81355870 None:intergenic 15.0%
!!! TAAAAAATTAAAGTATTACC+CGG - chr6_4:81355851-81355870 None:intergenic 15.0%
!!! TTTAGTTTTTTTTACTTCAG+CGG - chr6_4:81355202-81355221 None:intergenic 20.0%
!! TTAATTAGAATATCAAAGTC+GGG - chr6_4:81355231-81355250 None:intergenic 20.0%
!! GTTAATTAGAATATCAAAGT+CGG - chr6_4:81355232-81355251 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG - chr6_4:81355738-81355757 None:intergenic 20.0%
!!! GTCTTCATTTTTAAATTCAA+AGG - chr6_4:81355729-81355748 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG + chr6_4:81355738-81355757 Msa0999390:intron 20.0%
!!! ACTTTAATTTTTTACTTCAG+TGG + chr6_4:81355857-81355876 Msa0999390:CDS 20.0%
!!! TTTAGTTTTTTTTACTTCAG+CGG - chr6_4:81355202-81355221 None:intergenic 20.0%
!! TTAATTAGAATATCAAAGTC+GGG - chr6_4:81355231-81355250 None:intergenic 20.0%
!! GTTAATTAGAATATCAAAGT+CGG - chr6_4:81355232-81355251 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG - chr6_4:81355738-81355757 None:intergenic 20.0%
!!! GTCTTCATTTTTAAATTCAA+AGG - chr6_4:81355729-81355748 None:intergenic 20.0%
!!! AAAATGAAGACATTGATTAT+TGG + chr6_4:81355738-81355757 Msa0999390:intron 20.0%
!!! ACTTTAATTTTTTACTTCAG+TGG + chr6_4:81355857-81355876 Msa0999390:CDS 20.0%
! TTAGCATCAATACAAAAATC+AGG + chr6_4:81355276-81355295 Msa0999390:intron 25.0%
!! AGAGAATTGCTAATAACTTT+TGG - chr6_4:81355351-81355370 None:intergenic 25.0%
! GAAAAAAAAATTAGGCGTTA+CGG + chr6_4:81355572-81355591 Msa0999390:intron 25.0%
! GTTAATTCGATTATTAAAGC+CGG + chr6_4:81355829-81355848 Msa0999390:CDS 25.0%
! TTAATTCGATTATTAAAGCC+GGG + chr6_4:81355830-81355849 Msa0999390:CDS 25.0%
!!! ATTTTTTACTTCAGTGGAAT+TGG + chr6_4:81355863-81355882 Msa0999390:CDS 25.0%
! TTGGAAATTATCAAGAAGAA+TGG + chr6_4:81355882-81355901 Msa0999390:CDS 25.0%
! TTAGCATCAATACAAAAATC+AGG + chr6_4:81355276-81355295 Msa0999390:intron 25.0%
!! AGAGAATTGCTAATAACTTT+TGG - chr6_4:81355351-81355370 None:intergenic 25.0%
! GAAAAAAAAATTAGGCGTTA+CGG + chr6_4:81355572-81355591 Msa0999390:intron 25.0%
! GTTAATTCGATTATTAAAGC+CGG + chr6_4:81355829-81355848 Msa0999390:CDS 25.0%
! TTAATTCGATTATTAAAGCC+GGG + chr6_4:81355830-81355849 Msa0999390:CDS 25.0%
!!! ATTTTTTACTTCAGTGGAAT+TGG + chr6_4:81355863-81355882 Msa0999390:CDS 25.0%
! TTGGAAATTATCAAGAAGAA+TGG + chr6_4:81355882-81355901 Msa0999390:CDS 25.0%
TTGTAAAAGTTACCATGTAG+AGG + chr6_4:81355121-81355140 Msa0999390:CDS 30.0%
CATGGAAATTATCAAGAAGA+AGG - chr6_4:81355178-81355197 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG + chr6_4:81355359-81355378 Msa0999390:intron 30.0%
TCGGAATATGTGTTAATTGT+AGG + chr6_4:81355378-81355397 Msa0999390:intron 30.0%
ATGTGTTAATTGTAGGTTCT+CGG + chr6_4:81355385-81355404 Msa0999390:intron 30.0%
AGGTGTTAGTTGTAAGTTAT+CGG - chr6_4:81355676-81355695 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG - chr6_4:81355359-81355378 None:intergenic 30.0%
CTGTAAAAGTTAACATGTAG+AGG - chr6_4:81355938-81355957 None:intergenic 30.0%
TTGTAAAAGTTACCATGTAG+AGG + chr6_4:81355121-81355140 Msa0999390:CDS 30.0%
CATGGAAATTATCAAGAAGA+AGG - chr6_4:81355178-81355197 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG + chr6_4:81355359-81355378 Msa0999390:intron 30.0%
TCGGAATATGTGTTAATTGT+AGG + chr6_4:81355378-81355397 Msa0999390:intron 30.0%
ATGTGTTAATTGTAGGTTCT+CGG + chr6_4:81355385-81355404 Msa0999390:intron 30.0%
AGGTGTTAGTTGTAAGTTAT+CGG - chr6_4:81355676-81355695 None:intergenic 30.0%
TAGCAATTCTCTTATCTTCT+CGG - chr6_4:81355359-81355378 None:intergenic 30.0%
CTGTAAAAGTTAACATGTAG+AGG - chr6_4:81355938-81355957 None:intergenic 30.0%
!!! TTTTTTTACTTCAGCGGACA+TGG - chr6_4:81355196-81355215 None:intergenic 35.0%
TTCTCTTATCTTCTCGGAAT+AGG - chr6_4:81355365-81355384 None:intergenic 35.0%
AATCAAGTCCTTCATCGTTT+AGG - chr6_4:81355769-81355788 None:intergenic 35.0%
!!! TTTTTTTACTTCAGCGGACA+TGG - chr6_4:81355196-81355215 None:intergenic 35.0%
TTCTCTTATCTTCTCGGAAT+AGG - chr6_4:81355365-81355384 None:intergenic 35.0%
AATCAAGTCCTTCATCGTTT+AGG - chr6_4:81355769-81355788 None:intergenic 35.0%
ACCATGTAGAGGAGTTCAAT+CGG + chr6_4:81355132-81355151 Msa0999390:CDS 40.0%
! TAGGCGTTACGGACAAATTT+TGG + chr6_4:81355583-81355602 Msa0999390:intron 40.0%
CAAAGTTACCTTATTGCAGC+CGG + chr6_4:81355905-81355924 Msa0999390:CDS 40.0%
ACCATGTAGAGGAGTTCAAT+CGG + chr6_4:81355132-81355151 Msa0999390:CDS 40.0%
! TAGGCGTTACGGACAAATTT+TGG + chr6_4:81355583-81355602 Msa0999390:intron 40.0%
CAAAGTTACCTTATTGCAGC+CGG + chr6_4:81355905-81355924 Msa0999390:CDS 40.0%
TGGCAAGACAGAAACGATGA+AGG - chr6_4:81355303-81355322 None:intergenic 45.0%
TGGCAAGACCTAAACGATGA+AGG + chr6_4:81355758-81355777 Msa0999390:CDS 45.0%
AACATGTAGAGGAGTTCCAC+CGG - chr6_4:81355927-81355946 None:intergenic 45.0%
TGGCAAGACAGAAACGATGA+AGG - chr6_4:81355303-81355322 None:intergenic 45.0%
TGGCAAGACCTAAACGATGA+AGG + chr6_4:81355758-81355777 Msa0999390:CDS 45.0%
AACATGTAGAGGAGTTCCAC+CGG - chr6_4:81355927-81355946 None:intergenic 45.0%
GCCGATTGAACTCCTCTACA+TGG - chr6_4:81355136-81355155 None:intergenic 50.0%
GAGTTCAATCGGCTGCAACA+AGG + chr6_4:81355143-81355162 Msa0999390:CDS 50.0%
AGTTACCTTATTGCAGCCGG+TGG + chr6_4:81355908-81355927 Msa0999390:CDS 50.0%
GCCGATTGAACTCCTCTACA+TGG - chr6_4:81355136-81355155 None:intergenic 50.0%
GAGTTCAATCGGCTGCAACA+AGG + chr6_4:81355143-81355162 Msa0999390:CDS 50.0%
AGTTACCTTATTGCAGCCGG+TGG + chr6_4:81355908-81355927 Msa0999390:CDS 50.0%
! GAGTTCCACCGGCTGCAATA+AGG - chr6_4:81355916-81355935 None:intergenic 55.0%
! GAGTTCCACCGGCTGCAATA+AGG - chr6_4:81355916-81355935 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81355107 81355997 81355107 ID=Msa0999390;Name=Msa0999390
chr6_4 mRNA 81355107 81355997 81355107 ID=Msa0999390-mRNA-1;Parent=Msa0999390;Name=Msa0999390-mRNA-1;_AED=0.52;_eAED=0.62;_QI=0|0|0|0.5|0|0|2|0|102
chr6_4 exon 81355107 81355164 81355107 ID=Msa0999390-mRNA-1:exon:15556;Parent=Msa0999390-mRNA-1
chr6_4 exon 81355747 81355997 81355747 ID=Msa0999390-mRNA-1:exon:15557;Parent=Msa0999390-mRNA-1
chr6_4 CDS 81355107 81355164 81355107 ID=Msa0999390-mRNA-1:cds;Parent=Msa0999390-mRNA-1
chr6_4 CDS 81355747 81355997 81355747 ID=Msa0999390-mRNA-1:cds;Parent=Msa0999390-mRNA-1
Gene Sequence

>Msa0999390

ATGAATGAGATGTATTGTAAAAGTTACCATGTAGAGGAGTTCAATCGGCTGCAACAAGACATTGATTATTGGCAAGACCTAAACGATGAAGGACTTGATTTTCGTATTGATGTTAAGAAAATATATGAAAGATTAACTCAGTTAATTCGATTATTAAAGCCGGGTAATACTTTAATTTTTTACTTCAGTGGAATTGGAAATTATCAAGAAGAATGGCAAAGTTACCTTATTGCAGCCGGTGGAACTCCTCTACATGTTAACTTTTACAGCACGTCTCATGCATATATTAATTTTATTTTCAACTCTTAA

Protein sequence

>Msa0999390

MNEMYCKSYHVEEFNRLQQDIDYWQDLNDEGLDFRIDVKKIYERLTQLIRLLKPGNTLIFYFSGIGNYQEEWQSYLIAAGGTPLHVNFYSTSHAYINFIFNS*