AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1219820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1219820 MtrunA17_Chr4g0069801 97.566 493 11 1 1 493 1 492 0.0 992
Msa1219820 MtrunA17_Chr1g0148391 57.456 456 189 3 1 455 1 452 0.0 526
Msa1219820 MtrunA17_Chr3g0138741 53.958 480 216 3 4 481 8 484 1.35e-180 515
Msa1219820 MtrunA17_Chr6g0461601 51.509 464 211 7 1 456 1 458 4.63e-161 465
Msa1219820 MtrunA17_Chr4g0062771 49.889 451 216 6 11 455 14 460 2.53e-159 461
Msa1219820 MtrunA17_Chr4g0076581 50.321 467 220 6 31 493 36 494 2.35e-157 456
Msa1219820 MtrunA17_Chr8g0383311 48.018 454 229 4 6 455 20 470 1.42e-156 454
Msa1219820 MtrunA17_Chr2g0290821 52.055 438 202 5 31 464 31 464 3.05e-156 453
Msa1219820 MtrunA17_Chr7g0253091 52.009 448 206 5 27 469 25 468 7.38e-156 452
Msa1219820 MtrunA17_Chr2g0306311 44.190 525 246 8 1 492 1 511 1.24e-150 439
Msa1219820 MtrunA17_Chr3g0129341 45.661 484 242 8 13 481 10 487 1.84e-138 407
Msa1219820 MtrunA17_Chr3g0105981 44.819 415 224 4 21 434 20 430 1.28e-121 362
Msa1219820 MtrunA17_Chr4g0046311 32.903 465 277 18 14 454 9 462 1.23e-67 224
Msa1219820 MtrunA17_Chr8g0339191 34.989 443 260 17 30 455 31 462 4.09e-65 218
Msa1219820 MtrunA17_Chr3g0144531 33.260 457 273 16 3 443 8 448 2.13e-64 215
Msa1219820 MtrunA17_Chr4g0034901 32.527 455 276 16 10 443 9 453 1.11e-63 214
Msa1219820 MtrunA17_Chr1g0161011 33.480 454 275 16 11 451 6 445 1.00e-59 204
Msa1219820 MtrunA17_Chr4g0003281 36.389 360 206 13 7 351 12 363 4.35e-59 200
Msa1219820 MtrunA17_Chr5g0434591 31.729 457 280 17 10 451 5 444 2.95e-58 200
Msa1219820 MtrunA17_Chr2g0294601 34.181 354 221 7 1 352 33 376 7.29e-58 196
Msa1219820 MtrunA17_Chr7g0234821 32.184 435 259 19 39 451 38 458 1.71e-55 192
Msa1219820 MtrunA17_Chr1g0177171 29.638 469 275 18 24 451 36 490 3.52e-55 192
Msa1219820 MtrunA17_Chr2g0294591 36.218 312 189 6 40 349 41 344 4.83e-55 188
Msa1219820 MtrunA17_Chr3g0121271 28.571 462 299 16 1 449 1 444 1.87e-54 190
Msa1219820 MtrunA17_Chr8g0378621 30.416 457 289 16 10 451 6 448 2.80e-54 189
Msa1219820 MtrunA17_Chr3g0110371 30.819 464 276 21 10 451 5 445 5.74e-54 189
Msa1219820 MtrunA17_Chr4g0039071 33.861 316 198 7 32 345 29 335 8.02e-54 185
Msa1219820 MtrunA17_Chr5g0439251 30.573 471 279 18 24 459 23 480 9.34e-54 188
Msa1219820 MtrunA17_Chr6g0487261 30.682 440 270 19 41 462 40 462 3.79e-52 184
Msa1219820 MtrunA17_Chr8g0389741 29.647 425 275 14 39 451 40 452 1.04e-51 183
Msa1219820 MtrunA17_Chr7g0250301 37.037 324 189 10 25 345 5 316 1.57e-51 177
Msa1219820 MtrunA17_Chr7g0223961 30.254 433 266 19 31 443 26 442 1.57e-51 181
Msa1219820 MtrunA17_Chr1g0146831 34.218 339 202 13 14 343 17 343 2.31e-50 176
Msa1219820 MtrunA17_Chr4g0064021 34.706 340 202 12 20 349 18 347 5.10e-50 175
Msa1219820 MtrunA17_Chr4g0075031 28.375 437 279 14 31 451 48 466 2.18e-49 177
Msa1219820 MtrunA17_Chr4g0038961 34.211 342 205 12 7 345 2 326 2.08e-48 169
Msa1219820 MtrunA17_Chr3g0119541 33.724 341 209 11 21 352 20 352 4.37e-48 169
Msa1219820 MtrunA17_Chr1g0174261 34.948 289 177 8 57 343 1 280 2.05e-47 167
Msa1219820 MtrunA17_Chr4g0043851 31.722 331 209 12 27 349 31 352 2.40e-47 169
Msa1219820 MtrunA17_Chr4g0038981 32.268 313 196 8 32 343 27 324 3.55e-47 166
Msa1219820 MtrunA17_Chr4g0026321 29.787 376 230 15 26 391 27 378 6.82e-47 167
Msa1219820 MtrunA17_Chr2g0321511 32.051 390 228 19 7 381 5 372 7.70e-47 167
Msa1219820 MtrunA17_Chr4g0038971 32.558 344 210 13 6 345 1 326 9.24e-47 165
Msa1219820 MtrunA17_Chr4g0038941 34.091 308 183 10 43 347 67 357 1.08e-46 166
Msa1219820 MtrunA17_Chr1g0189111 35.312 337 196 14 21 348 24 347 1.90e-46 167
Msa1219820 MtrunA17_Chr1g0155001 32.099 324 205 9 28 343 24 340 4.17e-46 166
Msa1219820 MtrunA17_Chr4g0039021 33.775 302 184 9 48 345 1 290 1.41e-45 161
Msa1219820 MtrunA17_Chr4g0039011 29.863 365 220 11 6 349 1 350 6.78e-43 155
Msa1219820 MtrunA17_Chr7g0250321 36.735 294 175 8 1 292 1 285 1.01e-41 150
Msa1219820 MtrunA17_Chr4g0043511 26.237 465 299 16 18 451 52 503 2.38e-41 155
Msa1219820 MtrunA17_Chr2g0285901 30.836 347 218 15 8 343 2 337 4.00e-40 149
Msa1219820 MtrunA17_Chr5g0436361 28.477 302 198 10 49 343 58 348 1.34e-27 114
Msa1219820 MtrunA17_Chr2g0294581 32.143 196 114 5 40 226 87 272 9.23e-21 92.4
Msa1219820 MtrunA17_Chr1g0213651 40.909 88 46 4 366 451 276 359 8.39e-12 66.6
Msa1219820 MtrunA17_Chr4g0018861 34.783 92 55 3 256 346 87 174 3.40e-11 63.2
Msa1219820 MtrunA17_Chr4g0018771 33.333 96 59 3 251 345 84 175 8.49e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1219820 AT5G20870.1 61.475 488 170 6 10 482 13 497 0.0 611
Msa1219820 AT4G31140.1 56.067 478 203 3 6 483 7 477 0.0 534
Msa1219820 AT5G58090.1 58.178 428 175 2 31 456 25 450 0.0 523
Msa1219820 AT2G19440.2 54.378 434 188 6 27 455 20 448 5.92e-162 468
Msa1219820 AT2G19440.1 54.378 434 188 6 27 455 20 448 5.92e-162 468
Msa1219820 AT1G64760.1 53.917 434 190 6 27 455 24 452 8.28e-161 465
Msa1219820 AT1G64760.2 53.917 434 190 6 27 455 24 452 8.28e-161 465
Msa1219820 AT5G64790.1 51.025 439 209 4 20 455 20 455 5.85e-157 455
Msa1219820 AT3G24330.1 49.781 456 218 7 9 458 21 471 1.95e-154 449
Msa1219820 AT3G04010.1 50.670 448 208 6 13 455 22 461 3.70e-152 443
Msa1219820 AT5G18220.1 49.458 461 218 8 4 455 5 459 4.42e-150 437
Msa1219820 AT4G17180.1 48.344 453 216 8 10 458 10 448 2.48e-147 430
Msa1219820 AT5G58480.1 42.694 438 244 5 31 467 29 460 1.90e-125 374
Msa1219820 AT2G01630.1 32.874 435 262 17 31 451 26 444 6.28e-66 221
Msa1219820 AT4G26830.1 32.543 464 271 18 10 451 8 451 1.04e-65 219
Msa1219820 AT4G26830.2 32.957 443 256 18 31 451 32 455 1.99e-65 218
Msa1219820 AT2G01630.3 32.787 427 259 16 31 443 26 438 2.05e-65 218
Msa1219820 AT5G55180.1 31.900 442 265 18 31 451 30 456 4.17e-65 218
Msa1219820 AT2G05790.1 32.026 459 268 17 24 451 23 468 9.53e-65 217
Msa1219820 AT5G55180.2 31.954 435 262 17 30 443 29 450 9.03e-64 214
Msa1219820 AT4G34480.1 33.784 444 272 14 10 443 10 441 1.02e-62 213
Msa1219820 AT5G24318.1 30.501 459 280 21 13 452 18 456 1.99e-60 205
Msa1219820 AT5G24318.2 30.667 450 275 20 13 443 18 449 1.70e-59 202
Msa1219820 AT2G16230.1 30.977 481 299 17 31 492 28 494 1.18e-58 202
Msa1219820 AT3G57270.1 37.942 311 181 8 39 347 39 339 3.25e-58 196
Msa1219820 AT1G66250.1 31.278 454 286 14 28 470 32 470 1.33e-57 199
Msa1219820 AT3G55430.1 33.182 440 253 21 31 451 32 449 1.58e-56 195
Msa1219820 AT1G11820.2 29.638 442 275 15 26 451 44 465 1.87e-56 196
Msa1219820 AT5G56590.1 29.978 447 281 16 39 469 38 468 1.44e-55 194
Msa1219820 AT3G23770.1 31.757 444 268 19 31 451 40 471 3.47e-55 192
Msa1219820 AT1G11820.3 29.263 434 273 14 26 443 44 459 3.58e-55 192
Msa1219820 AT3G13560.4 32.553 427 259 16 31 443 29 440 6.39e-55 192
Msa1219820 AT3G13560.1 32.553 427 259 16 31 443 29 440 6.39e-55 192
Msa1219820 AT3G13560.2 32.553 427 259 16 31 443 29 440 6.39e-55 192
Msa1219820 AT3G13560.5 32.553 427 259 16 31 443 29 440 6.39e-55 192
Msa1219820 AT3G13560.3 32.553 427 259 16 31 443 29 440 6.39e-55 192
Msa1219820 AT3G07320.1 32.311 424 258 18 46 451 43 455 6.93e-54 188
Msa1219820 AT4G16260.2 35.556 315 189 9 40 351 6 309 1.74e-52 180
Msa1219820 AT4G16260.1 35.556 315 189 9 40 351 35 338 2.27e-52 181
Msa1219820 AT4G14080.1 31.754 422 261 15 39 442 54 466 3.44e-52 184
Msa1219820 AT2G01630.2 33.531 337 204 12 30 355 25 352 1.71e-51 180
Msa1219820 AT3G57260.1 35.691 311 182 11 39 347 44 338 2.33e-51 178
Msa1219820 AT3G57260.2 35.691 311 182 11 39 347 60 354 2.99e-51 178
Msa1219820 AT4G34480.2 35.328 351 209 11 10 352 10 350 6.37e-51 177
Msa1219820 AT3G15800.1 33.526 346 214 11 16 352 30 368 1.68e-50 177
Msa1219820 AT1G32860.1 33.616 354 212 15 3 345 6 347 2.39e-50 177
Msa1219820 AT2G27500.2 34.441 331 201 11 27 349 28 350 2.13e-49 174
Msa1219820 AT2G27500.1 34.441 331 201 11 27 349 28 350 4.91e-49 173
Msa1219820 AT4G29360.1 28.160 451 276 14 39 453 39 477 2.26e-48 175
Msa1219820 AT4G29360.2 28.074 488 295 16 10 453 12 487 2.56e-48 175
Msa1219820 AT2G39640.1 30.508 472 287 21 10 466 12 457 4.37e-48 174
Msa1219820 AT5G24318.4 32.153 339 208 15 13 343 18 342 2.76e-47 168
Msa1219820 AT2G26600.1 33.134 335 204 12 28 352 34 358 1.29e-46 167
Msa1219820 AT1G30080.2 29.014 355 229 12 3 343 5 350 2.50e-44 160
Msa1219820 AT1G77780.1 33.887 301 183 11 39 336 36 323 3.27e-44 159
Msa1219820 AT1G30080.1 29.014 355 229 12 3 343 5 350 4.29e-44 160
Msa1219820 AT1G77790.1 32.565 347 216 11 5 345 7 341 5.63e-44 158
Msa1219820 AT4G34480.3 33.929 336 204 12 118 443 3 330 6.15e-44 159
Msa1219820 AT4G18340.2 30.084 359 225 15 3 348 6 351 3.62e-43 157
Msa1219820 AT4G18340.1 30.084 359 225 15 3 348 6 351 6.55e-43 157
Msa1219820 AT5G42720.1 31.909 351 220 12 6 347 6 346 1.44e-42 157
Msa1219820 AT3G61810.1 31.487 343 208 13 18 348 45 372 2.04e-42 155
Msa1219820 AT3G57240.1 32.476 311 188 10 41 347 48 340 6.36e-42 153
Msa1219820 AT5G42100.2 30.814 344 218 13 14 347 15 348 1.57e-41 154
Msa1219820 AT5G42100.1 30.814 344 218 13 14 347 15 348 2.12e-41 153
Msa1219820 AT1G11820.1 29.641 334 217 10 26 350 44 368 3.17e-40 149
Msa1219820 AT1G33220.1 29.321 324 214 10 3 321 9 322 1.21e-39 146
Msa1219820 AT5G20390.1 29.499 339 216 10 9 336 7 333 1.78e-39 146
Msa1219820 AT2G27500.3 34.050 279 170 9 78 349 1 272 1.02e-38 143
Msa1219820 AT5G24318.3 30.000 330 200 14 141 452 1 317 1.28e-38 143
Msa1219820 AT5G20560.1 29.560 318 195 10 30 336 36 335 1.48e-37 141
Msa1219820 AT5G20330.1 28.319 339 225 11 3 336 9 334 7.50e-36 136
Msa1219820 AT5G20340.1 29.841 315 197 13 30 336 45 343 1.42e-35 136
Msa1219820 AT2G26600.2 34.066 273 162 10 89 352 1 264 1.05e-34 132
Msa1219820 AT3G46570.1 30.029 343 217 13 10 343 12 340 2.39e-32 127
Msa1219820 AT3G55780.1 25.857 321 217 10 41 351 49 358 3.37e-26 110

Find 107 sgRNAs with CRISPR-Local

Find 317 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATTCTTTAGGGAAATT+AGG 0.162535 8_1:+82716063 None:intergenic
TCAAAGAAAGCAAATTCTTT+AGG 0.203190 8_1:+82716054 None:intergenic
CTCATACTTTGTGCAAAATT+AGG 0.205482 8_1:+82715577 None:intergenic
AAAGAATTTGCTTTCTTTGA+TGG 0.208174 8_1:-82716051 Msa1219820:CDS
CCCTTGGCTTTGCTTGCAAT+TGG 0.238585 8_1:-82717032 Msa1219820:CDS
TTTGAGATCATGATTGATAT+TGG 0.242623 8_1:-82715358 Msa1219820:CDS
ATGTAAGAGGGCCGTTATTT+TGG 0.244858 8_1:+82716121 None:intergenic
CTGAAGGCTCTTGGGAATTC+TGG 0.246366 8_1:-82716907 Msa1219820:CDS
TCTTGGGAATTCTGGCATTC+AGG 0.280623 8_1:-82716899 Msa1219820:CDS
ATGAAATACCCTTTGAATCT+TGG 0.284657 8_1:-82715688 Msa1219820:CDS
CAAAGAAAGCAAATTCTTTA+GGG 0.289026 8_1:+82716055 None:intergenic
CCTTGGCTTTGCTTGCAATT+GGG 0.291854 8_1:-82717031 Msa1219820:CDS
AGGGCAAACATGTAAATTTC+AGG 0.292702 8_1:+82715787 None:intergenic
GAAATAACACACCATTGTTT+TGG 0.299535 8_1:+82715622 None:intergenic
GAATTCTGGCATTCAGGTTA+TGG 0.301145 8_1:-82716893 Msa1219820:CDS
TGGTAGACATCAGCATTTAA+AGG 0.322180 8_1:+82716225 None:intergenic
ATTTGATTATTGACATCATT+TGG 0.333649 8_1:+82716151 None:intergenic
TCTGCTGCCCCTGTAGTTGA+TGG 0.360256 8_1:-82716027 Msa1219820:CDS
ATATGAAGGCGGAAACATTT+TGG 0.364501 8_1:+82716803 None:intergenic
GGTAGACATCAGCATTTAAA+GGG 0.366946 8_1:+82716226 None:intergenic
TTGAGATCATGATTGATATT+GGG 0.371941 8_1:-82715357 Msa1219820:CDS
TCCTCTTCCAGCTCAAATAA+TGG 0.373231 8_1:-82716992 Msa1219820:CDS
TTGTATACTAAGCCAAAGTT+TGG 0.391479 8_1:-82717064 Msa1219820:CDS
ATTCCCCAATGTCTCTCAAA+TGG 0.397966 8_1:+82715730 None:intergenic
CCCATGGCACTGAAGGCTCT+TGG 0.409961 8_1:-82716916 Msa1219820:CDS
CTTGGCTTTGCTTGCAATTG+GGG 0.413284 8_1:-82717030 Msa1219820:CDS
AAATCACTAGTTGGTGCTAA+AGG 0.413423 8_1:-82715658 Msa1219820:CDS
AGCTTCACCATTATTTGAGC+TGG 0.418137 8_1:+82716985 None:intergenic
CCATGGCACTGAAGGCTCTT+GGG 0.419662 8_1:-82716915 Msa1219820:CDS
AATCACTAGTTGGTGCTAAA+GGG 0.420833 8_1:-82715657 Msa1219820:CDS
TGGCCCATTTGAGAGACATT+GGG 0.432301 8_1:-82715734 Msa1219820:CDS
AAAGATGCATGTCAATTCAA+TGG 0.443198 8_1:-82715433 Msa1219820:CDS
TTCATCTCAAGCAAATGTAA+TGG 0.443401 8_1:-82715602 Msa1219820:CDS
GGGTAGATGTTAAATGTAAG+AGG 0.443692 8_1:+82716108 None:intergenic
TAATATGTATTATCAAAGAA+TGG 0.448941 8_1:-82715464 Msa1219820:CDS
TGGCATACCCAATGACCTCT+TGG 0.450780 8_1:-82716869 Msa1219820:CDS
AATGTTATCATTGGTGAAGT+TGG 0.454844 8_1:-82715922 Msa1219820:CDS
GCATTTGATGTTCCATCAGT+TGG 0.457755 8_1:+82715895 None:intergenic
AGCTGATCCCATGGCACTGA+AGG 0.462871 8_1:-82716923 Msa1219820:CDS
CAAGCAGCCCTTATAAAAGC+AGG 0.466267 8_1:-82716276 Msa1219820:CDS
GGTTCAATGAATGTTATCAT+TGG 0.468945 8_1:-82715931 Msa1219820:CDS
AAGTGAATCCAAGAGGTCAT+TGG 0.477036 8_1:+82716861 None:intergenic
AGTGAATCCAAGAGGTCATT+GGG 0.485799 8_1:+82716862 None:intergenic
CTGGCCCATTTGAGAGACAT+TGG 0.488422 8_1:-82715735 Msa1219820:CDS
TCAGCATCAAGGCTTAGAAA+AGG 0.491244 8_1:+82716087 None:intergenic
AGTTTGGCAAAAGGTACCCT+TGG 0.491787 8_1:-82717048 Msa1219820:CDS
TTCCCCAATGTCTCTCAAAT+GGG 0.492714 8_1:+82715731 None:intergenic
ACCATTATTTGAGCTGGAAG+AGG 0.496228 8_1:+82716991 None:intergenic
CCCAAGAGCCTTCAGTGCCA+TGG 0.497598 8_1:+82716915 None:intergenic
GCTCCTGGCTCTTCTTGTAG+TGG 0.498871 8_1:-82715520 Msa1219820:CDS
GATTGTACATCTCTTGCTCC+TGG 0.501491 8_1:-82715535 Msa1219820:CDS
AAATTTCTTAGCCAAAATAA+CGG 0.503273 8_1:-82716132 Msa1219820:CDS
AGTGACACTGGTCTTCCATC+AGG 0.505930 8_1:-82716201 Msa1219820:CDS
GATATGGAACCATCAACTAC+AGG 0.506108 8_1:+82716018 None:intergenic
TACCCTTTGAATCTTGGTGG+TGG 0.509344 8_1:-82715682 Msa1219820:CDS
CAATGTCTCTCAAATGGGCC+AGG 0.515554 8_1:+82715736 None:intergenic
GCCCTTATAAAAGCAGGGTT+AGG 0.517583 8_1:-82716270 Msa1219820:CDS
TTTCCACCACCAAGATTCAA+AGG 0.519584 8_1:+82715679 None:intergenic
TCTGGCATTCAGGTTATGGT+TGG 0.523834 8_1:-82716889 Msa1219820:CDS
ATTCCCAGGTATGTTGCTGT+TGG 0.524304 8_1:-82716369 Msa1219820:intron
GGAAATTAGGATCAGCATCA+AGG 0.525105 8_1:+82716076 None:intergenic
ATGGAAGACCAGTGTCACTT+TGG 0.528047 8_1:+82716205 None:intergenic
CTAAGCCAAAGTTTGGCAAA+AGG 0.528146 8_1:-82717057 Msa1219820:CDS
AAAAGCACGTACTAAAACGT+TGG 0.532946 8_1:+82717097 None:intergenic
TGTAGTGGTTTAGACACAAA+GGG 0.535458 8_1:-82715505 Msa1219820:CDS
CAGCATCAAGGCTTAGAAAA+GGG 0.536287 8_1:+82716088 None:intergenic
CCCAATTGCAAGCAAAGCCA+AGG 0.538896 8_1:+82717031 None:intergenic
ATGTAAATTTCAGGTGGGGT+AGG 0.542978 8_1:+82715796 None:intergenic
AGAGTACATAATGGTCATGC+TGG 0.544525 8_1:-82715287 Msa1219820:CDS
CCAAGAGCCTTCAGTGCCAT+GGG 0.544609 8_1:+82716916 None:intergenic
AATTGGATTAGAGTACATAA+TGG 0.544687 8_1:-82715296 Msa1219820:CDS
AGACTATACAAATAGAGATA+AGG 0.546510 8_1:+82715246 None:intergenic
TTCCACCACCAAGATTCAAA+GGG 0.549004 8_1:+82715680 None:intergenic
GGTCTAAAGTGTCCACCTGA+TGG 0.549481 8_1:+82716186 None:intergenic
TGCAATAGCAGCATTGACAT+TGG 0.551848 8_1:+82716834 None:intergenic
AGTGCTCAAAGATTTAATCA+AGG 0.558025 8_1:-82715862 Msa1219820:CDS
TTAAATATTTGCCAAAACAA+TGG 0.559751 8_1:-82715633 Msa1219820:CDS
AGAAGGTGAAGGATCAATGT+TGG 0.571619 8_1:+82715396 None:intergenic
ATGTCAATGCTGCTATTGCA+TGG 0.571781 8_1:-82716831 Msa1219820:CDS
ATCACTAGTTGGTGCTAAAG+GGG 0.581029 8_1:-82715656 Msa1219820:CDS
AAATACCCTTTGAATCTTGG+TGG 0.581742 8_1:-82715685 Msa1219820:CDS
ATTGATCCTTCACCTTCTCA+AGG 0.584331 8_1:-82715391 Msa1219820:CDS
GTGAAGCTTATGAAAGACAA+TGG 0.584491 8_1:-82716970 Msa1219820:CDS
ATATGGAACCATCAACTACA+GGG 0.586294 8_1:+82716019 None:intergenic
TTGTAGTGGTTTAGACACAA+AGG 0.587590 8_1:-82715506 Msa1219820:CDS
GCAAACATGTAAATTTCAGG+TGG 0.588739 8_1:+82715790 None:intergenic
GGTGGTGGAAAATCACTAGT+TGG 0.601318 8_1:-82715667 Msa1219820:CDS
CAATGCTGCTATTGCATGGG+TGG 0.601494 8_1:-82716827 Msa1219820:CDS
TTATCATTGGTGAAGTTGGT+TGG 0.606188 8_1:-82715918 Msa1219820:CDS
CAAACATGTAAATTTCAGGT+GGG 0.606459 8_1:+82715791 None:intergenic
GGTAGATGTTAAATGTAAGA+GGG 0.611599 8_1:+82716109 None:intergenic
AAATGACAACTACCTTGAGA+AGG 0.612080 8_1:+82715379 None:intergenic
GATGTCTACCAAAGTGACAC+TGG 0.613469 8_1:-82716213 Msa1219820:CDS
AAACCACTACAAGAAGAGCC+AGG 0.623694 8_1:+82715517 None:intergenic
AAGCAGCCCTTATAAAAGCA+GGG 0.628845 8_1:-82716275 Msa1219820:CDS
GAAGTTGGTTGGCCAACTGA+TGG 0.629204 8_1:-82715907 Msa1219820:CDS
GGCCCATTTGAGAGACATTG+GGG 0.629502 8_1:-82715733 Msa1219820:CDS
AAACATGTAAATTTCAGGTG+GGG 0.634074 8_1:+82715792 None:intergenic
TGTCAATGCTGCTATTGCAT+GGG 0.635182 8_1:-82716830 Msa1219820:CDS
GACACTGGTCTTCCATCAGG+TGG 0.637692 8_1:-82716198 Msa1219820:CDS
GTTGTTTGAAGCTGATCCCA+TGG 0.646484 8_1:-82716932 Msa1219820:CDS
GTAGACATCAGCATTTAAAG+GGG 0.650986 8_1:+82716227 None:intergenic
CAACTACCTTGAGAAGGTGA+AGG 0.652284 8_1:+82715385 None:intergenic
CCAATTGCAAGCAAAGCCAA+GGG 0.668441 8_1:+82717032 None:intergenic
GAGATACAAGTAGCACAGTG+TGG 0.710703 8_1:+82715079 None:intergenic
TGGAAGCAAGTGAATCCAAG+AGG 0.710711 8_1:+82716854 None:intergenic
TATGGAACCATCAACTACAG+GGG 0.718530 8_1:+82716020 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACACAATATTTTTTATTTTT+TGG - chr8_1:82717049-82717068 Msa1219820:CDS 10.0%
!!! ACACAATATTTTTTATTTTT+TGG - chr8_1:82717049-82717068 Msa1219820:CDS 10.0%
!!! AAGTAACATTAATGTTTTAT+TGG - chr8_1:82715694-82715713 Msa1219820:CDS 15.0%
!! TAATATGTATTATCAAAGAA+TGG - chr8_1:82716771-82716790 Msa1219820:intron 15.0%
!!! ATCAATTACACATTTATTTT+TGG + chr8_1:82717097-82717116 None:intergenic 15.0%
!!! AAGTAACATTAATGTTTTAT+TGG - chr8_1:82715694-82715713 Msa1219820:CDS 15.0%
!! TAATATGTATTATCAAAGAA+TGG - chr8_1:82716771-82716790 Msa1219820:intron 15.0%
!!! ATCAATTACACATTTATTTT+TGG + chr8_1:82717097-82717116 None:intergenic 15.0%
!!! TTGATTTGACACAAAAAATT+AGG - chr8_1:82715523-82715542 Msa1219820:CDS 20.0%
!! TGATTTGACACAAAAAATTA+GGG - chr8_1:82715524-82715543 Msa1219820:CDS 20.0%
!! GAATTAATTGTACAAAACAT+TGG + chr8_1:82715850-82715869 None:intergenic 20.0%
!! ATTTGATTATTGACATCATT+TGG + chr8_1:82716087-82716106 None:intergenic 20.0%
!! AAATTTCTTAGCCAAAATAA+CGG - chr8_1:82716103-82716122 Msa1219820:CDS 20.0%
!! AAAATTTGCATCAAAAACAT+TGG + chr8_1:82716248-82716267 None:intergenic 20.0%
!!! TTTTAAATCCTCATCTAAAA+GGG + chr8_1:82716470-82716489 None:intergenic 20.0%
!!! TTTTTAAATCCTCATCTAAA+AGG + chr8_1:82716471-82716490 None:intergenic 20.0%
!! TTAAATATTTGCCAAAACAA+TGG - chr8_1:82716602-82716621 Msa1219820:intron 20.0%
!!! TTGATTTGACACAAAAAATT+AGG - chr8_1:82715523-82715542 Msa1219820:CDS 20.0%
!! TGATTTGACACAAAAAATTA+GGG - chr8_1:82715524-82715543 Msa1219820:CDS 20.0%
!! GAATTAATTGTACAAAACAT+TGG + chr8_1:82715850-82715869 None:intergenic 20.0%
!! ATTTGATTATTGACATCATT+TGG + chr8_1:82716087-82716106 None:intergenic 20.0%
!! AAATTTCTTAGCCAAAATAA+CGG - chr8_1:82716103-82716122 Msa1219820:CDS 20.0%
!! AAAATTTGCATCAAAAACAT+TGG + chr8_1:82716248-82716267 None:intergenic 20.0%
!!! TTTTAAATCCTCATCTAAAA+GGG + chr8_1:82716470-82716489 None:intergenic 20.0%
!!! TTTTTAAATCCTCATCTAAA+AGG + chr8_1:82716471-82716490 None:intergenic 20.0%
!! TTAAATATTTGCCAAAACAA+TGG - chr8_1:82716602-82716621 Msa1219820:intron 20.0%
! AGGAAGAAAAAAAGACAATA+TGG - chr8_1:82715111-82715130 Msa1219820:three_prime_UTR 25.0%
! GGAAGAAAAAAAGACAATAT+GGG - chr8_1:82715112-82715131 Msa1219820:three_prime_UTR 25.0%
! AAACGACTTATAATTTGAGA+CGG - chr8_1:82715762-82715781 Msa1219820:CDS 25.0%
!!! GCTTTTCTAAAAACATACAA+TGG - chr8_1:82715896-82715915 Msa1219820:CDS 25.0%
! CAAAGAAAGCAAATTCTTTA+GGG + chr8_1:82716183-82716202 None:intergenic 25.0%
! TCAAAGAAAGCAAATTCTTT+AGG + chr8_1:82716184-82716203 None:intergenic 25.0%
!! AAAGAATTTGCTTTCTTTGA+TGG - chr8_1:82716184-82716203 Msa1219820:CDS 25.0%
! AAAAACATTGGTGTAAGATA+TGG + chr8_1:82716236-82716255 None:intergenic 25.0%
! ATTTCAGCTCTTGAAAAAAA+TGG - chr8_1:82716277-82716296 Msa1219820:CDS 25.0%
!!! GATTTAAAAAGTATTGATCC+TGG - chr8_1:82716481-82716500 Msa1219820:intron 25.0%
!! TTTGAGATCATGATTGATAT+TGG - chr8_1:82716877-82716896 Msa1219820:CDS 25.0%
! TTGAGATCATGATTGATATT+GGG - chr8_1:82716878-82716897 Msa1219820:CDS 25.0%
!! ATGTACTCTAATCCAATTTT+TGG + chr8_1:82716937-82716956 None:intergenic 25.0%
!! AATTGGATTAGAGTACATAA+TGG - chr8_1:82716939-82716958 Msa1219820:CDS 25.0%
! AGACTATACAAATAGAGATA+AGG + chr8_1:82716992-82717011 None:intergenic 25.0%
!!! GTGACTCATTTTTTAATTCT+TGG - chr8_1:82717132-82717151 Msa1219820:five_prime_UTR 25.0%
! AGGAAGAAAAAAAGACAATA+TGG - chr8_1:82715111-82715130 Msa1219820:three_prime_UTR 25.0%
! GGAAGAAAAAAAGACAATAT+GGG - chr8_1:82715112-82715131 Msa1219820:three_prime_UTR 25.0%
! AAACGACTTATAATTTGAGA+CGG - chr8_1:82715762-82715781 Msa1219820:CDS 25.0%
!!! GCTTTTCTAAAAACATACAA+TGG - chr8_1:82715896-82715915 Msa1219820:CDS 25.0%
! CAAAGAAAGCAAATTCTTTA+GGG + chr8_1:82716183-82716202 None:intergenic 25.0%
! TCAAAGAAAGCAAATTCTTT+AGG + chr8_1:82716184-82716203 None:intergenic 25.0%
!! AAAGAATTTGCTTTCTTTGA+TGG - chr8_1:82716184-82716203 Msa1219820:CDS 25.0%
! AAAAACATTGGTGTAAGATA+TGG + chr8_1:82716236-82716255 None:intergenic 25.0%
! ATTTCAGCTCTTGAAAAAAA+TGG - chr8_1:82716277-82716296 Msa1219820:CDS 25.0%
!!! GATTTAAAAAGTATTGATCC+TGG - chr8_1:82716481-82716500 Msa1219820:intron 25.0%
!! TTTGAGATCATGATTGATAT+TGG - chr8_1:82716877-82716896 Msa1219820:CDS 25.0%
! TTGAGATCATGATTGATATT+GGG - chr8_1:82716878-82716897 Msa1219820:CDS 25.0%
!! ATGTACTCTAATCCAATTTT+TGG + chr8_1:82716937-82716956 None:intergenic 25.0%
!! AATTGGATTAGAGTACATAA+TGG - chr8_1:82716939-82716958 Msa1219820:CDS 25.0%
! AGACTATACAAATAGAGATA+AGG + chr8_1:82716992-82717011 None:intergenic 25.0%
!!! GTGACTCATTTTTTAATTCT+TGG - chr8_1:82717132-82717151 Msa1219820:five_prime_UTR 25.0%
TCAAAAATAGAAGCAAGTCA+AGG - chr8_1:82715091-82715110 Msa1219820:three_prime_UTR 30.0%
!! TTTTAGTACGTGCTTTTTGT+TGG - chr8_1:82715144-82715163 Msa1219820:three_prime_UTR 30.0%
TTGTATACTAAGCCAAAGTT+TGG - chr8_1:82715171-82715190 Msa1219820:three_prime_UTR 30.0%
!! AACCCCATTTTTAGATATGA+AGG + chr8_1:82715449-82715468 None:intergenic 30.0%
!! CTAAAAATGGGGTTGATATA+AGG - chr8_1:82715455-82715474 Msa1219820:CDS 30.0%
! ACTTATAATTTGAGACGGAT+GGG - chr8_1:82715767-82715786 Msa1219820:CDS 30.0%
ATAAGGATAATAATCTCACG+TGG - chr8_1:82715799-82715818 Msa1219820:CDS 30.0%
! GTTTTGTACAATTAATTCCC+AGG - chr8_1:82715852-82715871 Msa1219820:CDS 30.0%
!!! ATCATTTGGTTTTGAATGTC+TGG + chr8_1:82716073-82716092 None:intergenic 30.0%
GGTAGATGTTAAATGTAAGA+GGG + chr8_1:82716129-82716148 None:intergenic 30.0%
! GCAAATTCTTTAGGGAAATT+AGG + chr8_1:82716175-82716194 None:intergenic 30.0%
! GCTCTTGAAAAAAATGGTTT+TGG - chr8_1:82716283-82716302 Msa1219820:CDS 30.0%
GGTTCAATGAATGTTATCAT+TGG - chr8_1:82716304-82716323 Msa1219820:CDS 30.0%
AATGTTATCATTGGTGAAGT+TGG - chr8_1:82716313-82716332 Msa1219820:CDS 30.0%
AGTGCTCAAAGATTTAATCA+AGG - chr8_1:82716373-82716392 Msa1219820:intron 30.0%
GATAGAATAGTGAAAAAGCA+AGG - chr8_1:82716403-82716422 Msa1219820:intron 30.0%
! AAACATGTAAATTTCAGGTG+GGG + chr8_1:82716446-82716465 None:intergenic 30.0%
! CAAACATGTAAATTTCAGGT+GGG + chr8_1:82716447-82716466 None:intergenic 30.0%
! TGGGGAATTTTCAATTTTGA+TGG - chr8_1:82716520-82716539 Msa1219820:intron 30.0%
ATGAAATACCCTTTGAATCT+TGG - chr8_1:82716547-82716566 Msa1219820:intron 30.0%
! GAAATAACACACCATTGTTT+TGG + chr8_1:82716616-82716635 None:intergenic 30.0%
TTCATCTCAAGCAAATGTAA+TGG - chr8_1:82716633-82716652 Msa1219820:intron 30.0%
CTCATACTTTGTGCAAAATT+AGG + chr8_1:82716661-82716680 None:intergenic 30.0%
AAAGATGCATGTCAATTCAA+TGG - chr8_1:82716802-82716821 Msa1219820:CDS 30.0%
TCAAAAATAGAAGCAAGTCA+AGG - chr8_1:82715091-82715110 Msa1219820:three_prime_UTR 30.0%
!! TTTTAGTACGTGCTTTTTGT+TGG - chr8_1:82715144-82715163 Msa1219820:three_prime_UTR 30.0%
TTGTATACTAAGCCAAAGTT+TGG - chr8_1:82715171-82715190 Msa1219820:three_prime_UTR 30.0%
!! AACCCCATTTTTAGATATGA+AGG + chr8_1:82715449-82715468 None:intergenic 30.0%
!! CTAAAAATGGGGTTGATATA+AGG - chr8_1:82715455-82715474 Msa1219820:CDS 30.0%
! ACTTATAATTTGAGACGGAT+GGG - chr8_1:82715767-82715786 Msa1219820:CDS 30.0%
ATAAGGATAATAATCTCACG+TGG - chr8_1:82715799-82715818 Msa1219820:CDS 30.0%
! GTTTTGTACAATTAATTCCC+AGG - chr8_1:82715852-82715871 Msa1219820:CDS 30.0%
!!! ATCATTTGGTTTTGAATGTC+TGG + chr8_1:82716073-82716092 None:intergenic 30.0%
GGTAGATGTTAAATGTAAGA+GGG + chr8_1:82716129-82716148 None:intergenic 30.0%
! GCAAATTCTTTAGGGAAATT+AGG + chr8_1:82716175-82716194 None:intergenic 30.0%
! GCTCTTGAAAAAAATGGTTT+TGG - chr8_1:82716283-82716302 Msa1219820:CDS 30.0%
GGTTCAATGAATGTTATCAT+TGG - chr8_1:82716304-82716323 Msa1219820:CDS 30.0%
AATGTTATCATTGGTGAAGT+TGG - chr8_1:82716313-82716332 Msa1219820:CDS 30.0%
AGTGCTCAAAGATTTAATCA+AGG - chr8_1:82716373-82716392 Msa1219820:intron 30.0%
GATAGAATAGTGAAAAAGCA+AGG - chr8_1:82716403-82716422 Msa1219820:intron 30.0%
! AAACATGTAAATTTCAGGTG+GGG + chr8_1:82716446-82716465 None:intergenic 30.0%
! CAAACATGTAAATTTCAGGT+GGG + chr8_1:82716447-82716466 None:intergenic 30.0%
! TGGGGAATTTTCAATTTTGA+TGG - chr8_1:82716520-82716539 Msa1219820:intron 30.0%
ATGAAATACCCTTTGAATCT+TGG - chr8_1:82716547-82716566 Msa1219820:intron 30.0%
! GAAATAACACACCATTGTTT+TGG + chr8_1:82716616-82716635 None:intergenic 30.0%
TTCATCTCAAGCAAATGTAA+TGG - chr8_1:82716633-82716652 Msa1219820:intron 30.0%
CTCATACTTTGTGCAAAATT+AGG + chr8_1:82716661-82716680 None:intergenic 30.0%
AAAGATGCATGTCAATTCAA+TGG - chr8_1:82716802-82716821 Msa1219820:CDS 30.0%
! AAAAGCACGTACTAAAACGT+TGG + chr8_1:82715141-82715160 None:intergenic 35.0%
GTGAAGCTTATGAAAGACAA+TGG - chr8_1:82715265-82715284 Msa1219820:CDS 35.0%
! ATATGAAGGCGGAAACATTT+TGG + chr8_1:82715435-82715454 None:intergenic 35.0%
TCCGCCTTCATATCTAAAAA+TGG - chr8_1:82715442-82715461 Msa1219820:CDS 35.0%
!! CCCATTTTTAGATATGAAGG+CGG + chr8_1:82715446-82715465 None:intergenic 35.0%
CCGCCTTCATATCTAAAAAT+GGG - chr8_1:82715443-82715462 Msa1219820:CDS 35.0%
CGCCTTCATATCTAAAAATG+GGG - chr8_1:82715444-82715463 Msa1219820:CDS 35.0%
TGACCAAGGTAGTAATGAAA+AGG + chr8_1:82715502-82715521 None:intergenic 35.0%
! GACTTATAATTTGAGACGGA+TGG - chr8_1:82715766-82715785 Msa1219820:CDS 35.0%
! CTTATAATTTGAGACGGATG+GGG - chr8_1:82715768-82715787 Msa1219820:CDS 35.0%
!!! GCTTGAATGTTTTTTATGGC+TGG + chr8_1:82715944-82715963 None:intergenic 35.0%
!!! GGCTGCTTGAATGTTTTTTA+TGG + chr8_1:82715948-82715967 None:intergenic 35.0%
GTAGACATCAGCATTTAAAG+GGG + chr8_1:82716011-82716030 None:intergenic 35.0%
GGTAGACATCAGCATTTAAA+GGG + chr8_1:82716012-82716031 None:intergenic 35.0%
TGGTAGACATCAGCATTTAA+AGG + chr8_1:82716013-82716032 None:intergenic 35.0%
GGGTAGATGTTAAATGTAAG+AGG + chr8_1:82716130-82716149 None:intergenic 35.0%
ATATGGAACCATCAACTACA+GGG + chr8_1:82716219-82716238 None:intergenic 35.0%
! TTATCATTGGTGAAGTTGGT+TGG - chr8_1:82716317-82716336 Msa1219820:CDS 35.0%
GCAAACATGTAAATTTCAGG+TGG + chr8_1:82716448-82716467 None:intergenic 35.0%
AGGGCAAACATGTAAATTTC+AGG + chr8_1:82716451-82716470 None:intergenic 35.0%
AAATACCCTTTGAATCTTGG+TGG - chr8_1:82716550-82716569 Msa1219820:intron 35.0%
!! AAATCACTAGTTGGTGCTAA+AGG - chr8_1:82716577-82716596 Msa1219820:intron 35.0%
!! AATCACTAGTTGGTGCTAAA+GGG - chr8_1:82716578-82716597 Msa1219820:intron 35.0%
TTGTAGTGGTTTAGACACAA+AGG - chr8_1:82716729-82716748 Msa1219820:intron 35.0%
TGTAGTGGTTTAGACACAAA+GGG - chr8_1:82716730-82716749 Msa1219820:intron 35.0%
AAATGACAACTACCTTGAGA+AGG + chr8_1:82716859-82716878 None:intergenic 35.0%
! TCTTTTGCTGCACCAAAAAT+TGG - chr8_1:82716922-82716941 Msa1219820:CDS 35.0%
! AAAAGCACGTACTAAAACGT+TGG + chr8_1:82715141-82715160 None:intergenic 35.0%
GTGAAGCTTATGAAAGACAA+TGG - chr8_1:82715265-82715284 Msa1219820:CDS 35.0%
! ATATGAAGGCGGAAACATTT+TGG + chr8_1:82715435-82715454 None:intergenic 35.0%
TCCGCCTTCATATCTAAAAA+TGG - chr8_1:82715442-82715461 Msa1219820:CDS 35.0%
!! CCCATTTTTAGATATGAAGG+CGG + chr8_1:82715446-82715465 None:intergenic 35.0%
CCGCCTTCATATCTAAAAAT+GGG - chr8_1:82715443-82715462 Msa1219820:CDS 35.0%
CGCCTTCATATCTAAAAATG+GGG - chr8_1:82715444-82715463 Msa1219820:CDS 35.0%
TGACCAAGGTAGTAATGAAA+AGG + chr8_1:82715502-82715521 None:intergenic 35.0%
! GACTTATAATTTGAGACGGA+TGG - chr8_1:82715766-82715785 Msa1219820:CDS 35.0%
! CTTATAATTTGAGACGGATG+GGG - chr8_1:82715768-82715787 Msa1219820:CDS 35.0%
!!! GCTTGAATGTTTTTTATGGC+TGG + chr8_1:82715944-82715963 None:intergenic 35.0%
!!! GGCTGCTTGAATGTTTTTTA+TGG + chr8_1:82715948-82715967 None:intergenic 35.0%
GTAGACATCAGCATTTAAAG+GGG + chr8_1:82716011-82716030 None:intergenic 35.0%
GGTAGACATCAGCATTTAAA+GGG + chr8_1:82716012-82716031 None:intergenic 35.0%
TGGTAGACATCAGCATTTAA+AGG + chr8_1:82716013-82716032 None:intergenic 35.0%
GGGTAGATGTTAAATGTAAG+AGG + chr8_1:82716130-82716149 None:intergenic 35.0%
ATATGGAACCATCAACTACA+GGG + chr8_1:82716219-82716238 None:intergenic 35.0%
! TTATCATTGGTGAAGTTGGT+TGG - chr8_1:82716317-82716336 Msa1219820:CDS 35.0%
GCAAACATGTAAATTTCAGG+TGG + chr8_1:82716448-82716467 None:intergenic 35.0%
AGGGCAAACATGTAAATTTC+AGG + chr8_1:82716451-82716470 None:intergenic 35.0%
AAATACCCTTTGAATCTTGG+TGG - chr8_1:82716550-82716569 Msa1219820:intron 35.0%
!! AAATCACTAGTTGGTGCTAA+AGG - chr8_1:82716577-82716596 Msa1219820:intron 35.0%
!! AATCACTAGTTGGTGCTAAA+GGG - chr8_1:82716578-82716597 Msa1219820:intron 35.0%
TTGTAGTGGTTTAGACACAA+AGG - chr8_1:82716729-82716748 Msa1219820:intron 35.0%
TGTAGTGGTTTAGACACAAA+GGG - chr8_1:82716730-82716749 Msa1219820:intron 35.0%
AAATGACAACTACCTTGAGA+AGG + chr8_1:82716859-82716878 None:intergenic 35.0%
! TCTTTTGCTGCACCAAAAAT+TGG - chr8_1:82716922-82716941 Msa1219820:CDS 35.0%
CTAAGCCAAAGTTTGGCAAA+AGG - chr8_1:82715178-82715197 Msa1219820:three_prime_UTR 40.0%
TCCTCTTCCAGCTCAAATAA+TGG - chr8_1:82715243-82715262 Msa1219820:exon 40.0%
ACCATTATTTGAGCTGGAAG+AGG + chr8_1:82715247-82715266 None:intergenic 40.0%
AGCTTCACCATTATTTGAGC+TGG + chr8_1:82715253-82715272 None:intergenic 40.0%
! GAATTCTGGCATTCAGGTTA+TGG - chr8_1:82715342-82715361 Msa1219820:CDS 40.0%
AGTGAATCCAAGAGGTCATT+GGG + chr8_1:82715376-82715395 None:intergenic 40.0%
AAGTGAATCCAAGAGGTCAT+TGG + chr8_1:82715377-82715396 None:intergenic 40.0%
!! TGCAATAGCAGCATTGACAT+TGG + chr8_1:82715404-82715423 None:intergenic 40.0%
ATGTCAATGCTGCTATTGCA+TGG - chr8_1:82715404-82715423 Msa1219820:CDS 40.0%
TGTCAATGCTGCTATTGCAT+GGG - chr8_1:82715405-82715424 Msa1219820:CDS 40.0%
CCAAGGTAGTAATGAAAAGG+AGG + chr8_1:82715499-82715518 None:intergenic 40.0%
! CCTCCTTTTCATTACTACCT+TGG - chr8_1:82715496-82715515 Msa1219820:CDS 40.0%
TGTGTCAAATCAAGTGACCA+AGG + chr8_1:82715516-82715535 None:intergenic 40.0%
! CGGATGGGGTACTTATTATA+AGG - chr8_1:82715782-82715801 Msa1219820:CDS 40.0%
TTACCAACAGCAACATACCT+GGG + chr8_1:82715872-82715891 None:intergenic 40.0%
ATTACCAACAGCAACATACC+TGG + chr8_1:82715873-82715892 None:intergenic 40.0%
AAGCAGCCCTTATAAAAGCA+GGG - chr8_1:82715960-82715979 Msa1219820:CDS 40.0%
!! GCCTAACCCTGCTTTTATAA+GGG + chr8_1:82715969-82715988 None:intergenic 40.0%
!! TGCCTAACCCTGCTTTTATA+AGG + chr8_1:82715970-82715989 None:intergenic 40.0%
! ATGTAAGAGGGCCGTTATTT+TGG + chr8_1:82716117-82716136 None:intergenic 40.0%
CAGCATCAAGGCTTAGAAAA+GGG + chr8_1:82716150-82716169 None:intergenic 40.0%
TCAGCATCAAGGCTTAGAAA+AGG + chr8_1:82716151-82716170 None:intergenic 40.0%
GGAAATTAGGATCAGCATCA+AGG + chr8_1:82716162-82716181 None:intergenic 40.0%
TATGGAACCATCAACTACAG+GGG + chr8_1:82716218-82716237 None:intergenic 40.0%
GATATGGAACCATCAACTAC+AGG + chr8_1:82716220-82716239 None:intergenic 40.0%
GCATTTGATGTTCCATCAGT+TGG + chr8_1:82716343-82716362 None:intergenic 40.0%
! ATGTAAATTTCAGGTGGGGT+AGG + chr8_1:82716442-82716461 None:intergenic 40.0%
! CATGTTTGCCCTTTTAGATG+AGG - chr8_1:82716459-82716478 Msa1219820:intron 40.0%
TTCCCCAATGTCTCTCAAAT+GGG + chr8_1:82716507-82716526 None:intergenic 40.0%
ATTCCCCAATGTCTCTCAAA+TGG + chr8_1:82716508-82716527 None:intergenic 40.0%
TTCCACCACCAAGATTCAAA+GGG + chr8_1:82716558-82716577 None:intergenic 40.0%
TTTCCACCACCAAGATTCAA+AGG + chr8_1:82716559-82716578 None:intergenic 40.0%
!! ATCACTAGTTGGTGCTAAAG+GGG - chr8_1:82716579-82716598 Msa1219820:intron 40.0%
AGAAGGTGAAGGATCAATGT+TGG + chr8_1:82716842-82716861 None:intergenic 40.0%
ATTGATCCTTCACCTTCTCA+AGG - chr8_1:82716844-82716863 Msa1219820:CDS 40.0%
!! TTTTGGTGCAGCAAAAGAAG+TGG + chr8_1:82716920-82716939 None:intergenic 40.0%
AGAGTACATAATGGTCATGC+TGG - chr8_1:82716948-82716967 Msa1219820:CDS 40.0%
CTAAGCCAAAGTTTGGCAAA+AGG - chr8_1:82715178-82715197 Msa1219820:three_prime_UTR 40.0%
TCCTCTTCCAGCTCAAATAA+TGG - chr8_1:82715243-82715262 Msa1219820:exon 40.0%
ACCATTATTTGAGCTGGAAG+AGG + chr8_1:82715247-82715266 None:intergenic 40.0%
AGCTTCACCATTATTTGAGC+TGG + chr8_1:82715253-82715272 None:intergenic 40.0%
! GAATTCTGGCATTCAGGTTA+TGG - chr8_1:82715342-82715361 Msa1219820:CDS 40.0%
AGTGAATCCAAGAGGTCATT+GGG + chr8_1:82715376-82715395 None:intergenic 40.0%
AAGTGAATCCAAGAGGTCAT+TGG + chr8_1:82715377-82715396 None:intergenic 40.0%
!! TGCAATAGCAGCATTGACAT+TGG + chr8_1:82715404-82715423 None:intergenic 40.0%
ATGTCAATGCTGCTATTGCA+TGG - chr8_1:82715404-82715423 Msa1219820:CDS 40.0%
TGTCAATGCTGCTATTGCAT+GGG - chr8_1:82715405-82715424 Msa1219820:CDS 40.0%
CCAAGGTAGTAATGAAAAGG+AGG + chr8_1:82715499-82715518 None:intergenic 40.0%
! CCTCCTTTTCATTACTACCT+TGG - chr8_1:82715496-82715515 Msa1219820:CDS 40.0%
TGTGTCAAATCAAGTGACCA+AGG + chr8_1:82715516-82715535 None:intergenic 40.0%
! CGGATGGGGTACTTATTATA+AGG - chr8_1:82715782-82715801 Msa1219820:CDS 40.0%
TTACCAACAGCAACATACCT+GGG + chr8_1:82715872-82715891 None:intergenic 40.0%
ATTACCAACAGCAACATACC+TGG + chr8_1:82715873-82715892 None:intergenic 40.0%
AAGCAGCCCTTATAAAAGCA+GGG - chr8_1:82715960-82715979 Msa1219820:CDS 40.0%
!! GCCTAACCCTGCTTTTATAA+GGG + chr8_1:82715969-82715988 None:intergenic 40.0%
!! TGCCTAACCCTGCTTTTATA+AGG + chr8_1:82715970-82715989 None:intergenic 40.0%
! ATGTAAGAGGGCCGTTATTT+TGG + chr8_1:82716117-82716136 None:intergenic 40.0%
CAGCATCAAGGCTTAGAAAA+GGG + chr8_1:82716150-82716169 None:intergenic 40.0%
TCAGCATCAAGGCTTAGAAA+AGG + chr8_1:82716151-82716170 None:intergenic 40.0%
GGAAATTAGGATCAGCATCA+AGG + chr8_1:82716162-82716181 None:intergenic 40.0%
TATGGAACCATCAACTACAG+GGG + chr8_1:82716218-82716237 None:intergenic 40.0%
GATATGGAACCATCAACTAC+AGG + chr8_1:82716220-82716239 None:intergenic 40.0%
GCATTTGATGTTCCATCAGT+TGG + chr8_1:82716343-82716362 None:intergenic 40.0%
! ATGTAAATTTCAGGTGGGGT+AGG + chr8_1:82716442-82716461 None:intergenic 40.0%
! CATGTTTGCCCTTTTAGATG+AGG - chr8_1:82716459-82716478 Msa1219820:intron 40.0%
TTCCCCAATGTCTCTCAAAT+GGG + chr8_1:82716507-82716526 None:intergenic 40.0%
ATTCCCCAATGTCTCTCAAA+TGG + chr8_1:82716508-82716527 None:intergenic 40.0%
TTCCACCACCAAGATTCAAA+GGG + chr8_1:82716558-82716577 None:intergenic 40.0%
TTTCCACCACCAAGATTCAA+AGG + chr8_1:82716559-82716578 None:intergenic 40.0%
!! ATCACTAGTTGGTGCTAAAG+GGG - chr8_1:82716579-82716598 Msa1219820:intron 40.0%
AGAAGGTGAAGGATCAATGT+TGG + chr8_1:82716842-82716861 None:intergenic 40.0%
ATTGATCCTTCACCTTCTCA+AGG - chr8_1:82716844-82716863 Msa1219820:CDS 40.0%
!! TTTTGGTGCAGCAAAAGAAG+TGG + chr8_1:82716920-82716939 None:intergenic 40.0%
AGAGTACATAATGGTCATGC+TGG - chr8_1:82716948-82716967 Msa1219820:CDS 40.0%
! GGGTACCTTTTGCCAAACTT+TGG + chr8_1:82715186-82715205 None:intergenic 45.0%
!! AGTTTGGCAAAAGGTACCCT+TGG - chr8_1:82715187-82715206 Msa1219820:three_prime_UTR 45.0%
CCAATTGCAAGCAAAGCCAA+GGG + chr8_1:82715206-82715225 None:intergenic 45.0%
!! CCTTGGCTTTGCTTGCAATT+GGG - chr8_1:82715204-82715223 Msa1219820:three_prime_UTR 45.0%
!! CTTGGCTTTGCTTGCAATTG+GGG - chr8_1:82715205-82715224 Msa1219820:three_prime_UTR 45.0%
! GTTGTTTGAAGCTGATCCCA+TGG - chr8_1:82715303-82715322 Msa1219820:CDS 45.0%
TCTTGGGAATTCTGGCATTC+AGG - chr8_1:82715336-82715355 Msa1219820:CDS 45.0%
! TCTGGCATTCAGGTTATGGT+TGG - chr8_1:82715346-82715365 Msa1219820:CDS 45.0%
TGGAAGCAAGTGAATCCAAG+AGG + chr8_1:82715384-82715403 None:intergenic 45.0%
ATTCCCAGGTATGTTGCTGT+TGG - chr8_1:82715866-82715885 Msa1219820:CDS 45.0%
CAAGCAGCCCTTATAAAAGC+AGG - chr8_1:82715959-82715978 Msa1219820:CDS 45.0%
GCCCTTATAAAAGCAGGGTT+AGG - chr8_1:82715965-82715984 Msa1219820:CDS 45.0%
GATGTCTACCAAAGTGACAC+TGG - chr8_1:82716022-82716041 Msa1219820:CDS 45.0%
!! ATGGAAGACCAGTGTCACTT+TGG + chr8_1:82716033-82716052 None:intergenic 45.0%
TGGCCCATTTGAGAGACATT+GGG - chr8_1:82716501-82716520 Msa1219820:intron 45.0%
TACCCTTTGAATCTTGGTGG+TGG - chr8_1:82716553-82716572 Msa1219820:intron 45.0%
!! GGTGGTGGAAAATCACTAGT+TGG - chr8_1:82716568-82716587 Msa1219820:intron 45.0%
GATTGTACATCTCTTGCTCC+TGG - chr8_1:82716700-82716719 Msa1219820:intron 45.0%
AAACCACTACAAGAAGAGCC+AGG + chr8_1:82716721-82716740 None:intergenic 45.0%
CAACTACCTTGAGAAGGTGA+AGG + chr8_1:82716853-82716872 None:intergenic 45.0%
GAGATACAAGTAGCACAGTG+TGG + chr8_1:82717159-82717178 None:intergenic 45.0%
!! CTGTGCTACTTGTATCTCGA+CGG - chr8_1:82717161-82717180 Msa1219820:five_prime_UTR 45.0%
! GGGTACCTTTTGCCAAACTT+TGG + chr8_1:82715186-82715205 None:intergenic 45.0%
!! AGTTTGGCAAAAGGTACCCT+TGG - chr8_1:82715187-82715206 Msa1219820:three_prime_UTR 45.0%
CCAATTGCAAGCAAAGCCAA+GGG + chr8_1:82715206-82715225 None:intergenic 45.0%
!! CCTTGGCTTTGCTTGCAATT+GGG - chr8_1:82715204-82715223 Msa1219820:three_prime_UTR 45.0%
!! CTTGGCTTTGCTTGCAATTG+GGG - chr8_1:82715205-82715224 Msa1219820:three_prime_UTR 45.0%
! GTTGTTTGAAGCTGATCCCA+TGG - chr8_1:82715303-82715322 Msa1219820:CDS 45.0%
TCTTGGGAATTCTGGCATTC+AGG - chr8_1:82715336-82715355 Msa1219820:CDS 45.0%
! TCTGGCATTCAGGTTATGGT+TGG - chr8_1:82715346-82715365 Msa1219820:CDS 45.0%
TGGAAGCAAGTGAATCCAAG+AGG + chr8_1:82715384-82715403 None:intergenic 45.0%
ATTCCCAGGTATGTTGCTGT+TGG - chr8_1:82715866-82715885 Msa1219820:CDS 45.0%
CAAGCAGCCCTTATAAAAGC+AGG - chr8_1:82715959-82715978 Msa1219820:CDS 45.0%
GCCCTTATAAAAGCAGGGTT+AGG - chr8_1:82715965-82715984 Msa1219820:CDS 45.0%
GATGTCTACCAAAGTGACAC+TGG - chr8_1:82716022-82716041 Msa1219820:CDS 45.0%
!! ATGGAAGACCAGTGTCACTT+TGG + chr8_1:82716033-82716052 None:intergenic 45.0%
TGGCCCATTTGAGAGACATT+GGG - chr8_1:82716501-82716520 Msa1219820:intron 45.0%
TACCCTTTGAATCTTGGTGG+TGG - chr8_1:82716553-82716572 Msa1219820:intron 45.0%
!! GGTGGTGGAAAATCACTAGT+TGG - chr8_1:82716568-82716587 Msa1219820:intron 45.0%
GATTGTACATCTCTTGCTCC+TGG - chr8_1:82716700-82716719 Msa1219820:intron 45.0%
AAACCACTACAAGAAGAGCC+AGG + chr8_1:82716721-82716740 None:intergenic 45.0%
CAACTACCTTGAGAAGGTGA+AGG + chr8_1:82716853-82716872 None:intergenic 45.0%
GAGATACAAGTAGCACAGTG+TGG + chr8_1:82717159-82717178 None:intergenic 45.0%
!! CCCTTGGCTTTGCTTGCAAT+TGG - chr8_1:82715203-82715222 Msa1219820:three_prime_UTR 50.0%
CCCAATTGCAAGCAAAGCCA+AGG + chr8_1:82715207-82715226 None:intergenic 50.0%
CTGAAGGCTCTTGGGAATTC+TGG - chr8_1:82715328-82715347 Msa1219820:CDS 50.0%
TGGCATACCCAATGACCTCT+TGG - chr8_1:82715366-82715385 Msa1219820:CDS 50.0%
CAATGCTGCTATTGCATGGG+TGG - chr8_1:82715408-82715427 Msa1219820:CDS 50.0%
!! AGTGACACTGGTCTTCCATC+AGG - chr8_1:82716034-82716053 Msa1219820:CDS 50.0%
! GGTCTAAAGTGTCCACCTGA+TGG + chr8_1:82716052-82716071 None:intergenic 50.0%
! GAAGTTGGTTGGCCAACTGA+TGG - chr8_1:82716328-82716347 Msa1219820:CDS 50.0%
! TCAGGTGGGGTAGGTCTTTT+AGG + chr8_1:82716433-82716452 None:intergenic 50.0%
CAATGTCTCTCAAATGGGCC+AGG + chr8_1:82716502-82716521 None:intergenic 50.0%
CTGGCCCATTTGAGAGACAT+TGG - chr8_1:82716500-82716519 Msa1219820:intron 50.0%
GGCCCATTTGAGAGACATTG+GGG - chr8_1:82716502-82716521 Msa1219820:intron 50.0%
!! CCCTTGGCTTTGCTTGCAAT+TGG - chr8_1:82715203-82715222 Msa1219820:three_prime_UTR 50.0%
CCCAATTGCAAGCAAAGCCA+AGG + chr8_1:82715207-82715226 None:intergenic 50.0%
CTGAAGGCTCTTGGGAATTC+TGG - chr8_1:82715328-82715347 Msa1219820:CDS 50.0%
TGGCATACCCAATGACCTCT+TGG - chr8_1:82715366-82715385 Msa1219820:CDS 50.0%
CAATGCTGCTATTGCATGGG+TGG - chr8_1:82715408-82715427 Msa1219820:CDS 50.0%
!! AGTGACACTGGTCTTCCATC+AGG - chr8_1:82716034-82716053 Msa1219820:CDS 50.0%
! GGTCTAAAGTGTCCACCTGA+TGG + chr8_1:82716052-82716071 None:intergenic 50.0%
! GAAGTTGGTTGGCCAACTGA+TGG - chr8_1:82716328-82716347 Msa1219820:CDS 50.0%
! TCAGGTGGGGTAGGTCTTTT+AGG + chr8_1:82716433-82716452 None:intergenic 50.0%
CAATGTCTCTCAAATGGGCC+AGG + chr8_1:82716502-82716521 None:intergenic 50.0%
CTGGCCCATTTGAGAGACAT+TGG - chr8_1:82716500-82716519 Msa1219820:intron 50.0%
GGCCCATTTGAGAGACATTG+GGG - chr8_1:82716502-82716521 Msa1219820:intron 50.0%
! AGCTGATCCCATGGCACTGA+AGG - chr8_1:82715312-82715331 Msa1219820:CDS 55.0%
CCAAGAGCCTTCAGTGCCAT+GGG + chr8_1:82715322-82715341 None:intergenic 55.0%
! CCATGGCACTGAAGGCTCTT+GGG - chr8_1:82715320-82715339 Msa1219820:CDS 55.0%
!! GACACTGGTCTTCCATCAGG+TGG - chr8_1:82716037-82716056 Msa1219820:CDS 55.0%
TCTGCTGCCCCTGTAGTTGA+TGG - chr8_1:82716208-82716227 Msa1219820:CDS 55.0%
! GCTCCTGGCTCTTCTTGTAG+TGG - chr8_1:82716715-82716734 Msa1219820:intron 55.0%
! AGCTGATCCCATGGCACTGA+AGG - chr8_1:82715312-82715331 Msa1219820:CDS 55.0%
CCAAGAGCCTTCAGTGCCAT+GGG + chr8_1:82715322-82715341 None:intergenic 55.0%
! CCATGGCACTGAAGGCTCTT+GGG - chr8_1:82715320-82715339 Msa1219820:CDS 55.0%
!! GACACTGGTCTTCCATCAGG+TGG - chr8_1:82716037-82716056 Msa1219820:CDS 55.0%
TCTGCTGCCCCTGTAGTTGA+TGG - chr8_1:82716208-82716227 Msa1219820:CDS 55.0%
! GCTCCTGGCTCTTCTTGTAG+TGG - chr8_1:82716715-82716734 Msa1219820:intron 55.0%
! CCCATGGCACTGAAGGCTCT+TGG - chr8_1:82715319-82715338 Msa1219820:CDS 60.0%
CCCAAGAGCCTTCAGTGCCA+TGG + chr8_1:82715323-82715342 None:intergenic 60.0%
! CCCATGGCACTGAAGGCTCT+TGG - chr8_1:82715319-82715338 Msa1219820:CDS 60.0%
CCCAAGAGCCTTCAGTGCCA+TGG + chr8_1:82715323-82715342 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 82715076 82717181 82715076 ID=Msa1219820;Name=Msa1219820
chr8_1 mRNA 82715076 82717181 82715076 ID=Msa1219820-mRNA-1;Parent=Msa1219820;Name=Msa1219820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=54|1|1|1|1|1|2|173|493
chr8_1 exon 82715076 82716383 82715076 ID=Msa1219820-mRNA-1:exon:29571;Parent=Msa1219820-mRNA-1
chr8_1 exon 82716781 82717181 82716781 ID=Msa1219820-mRNA-1:exon:29570;Parent=Msa1219820-mRNA-1
chr8_1 five_prime_UTR 82717128 82717181 82717128 ID=Msa1219820-mRNA-1:five_prime_utr;Parent=Msa1219820-mRNA-1
chr8_1 CDS 82716781 82717127 82716781 ID=Msa1219820-mRNA-1:cds;Parent=Msa1219820-mRNA-1
chr8_1 CDS 82715249 82716383 82715249 ID=Msa1219820-mRNA-1:cds;Parent=Msa1219820-mRNA-1
chr8_1 three_prime_UTR 82715076 82715248 82715076 ID=Msa1219820-mRNA-1:three_prime_utr;Parent=Msa1219820-mRNA-1
Gene Sequence

>Msa1219820

ATGGGATTCCAACGTTTTAGTACGTGCTTTTTGTTGGCACTTTGTATACTAAGCCAAAGTTTGGCAAAAGGTACCCTTGGCTTTGCTTGCAATTGGGGAACGATTACATCGCATCCTCTTCCAGCTCAAATAATGGTGAAGCTTATGAAAGACAATGGAATCAACAAAGTGAAGTTGTTTGAAGCTGATCCCATGGCACTGAAGGCTCTTGGGAATTCTGGCATTCAGGTTATGGTTGGCATACCCAATGACCTCTTGGATTCACTTGCTTCCAATGTCAATGCTGCTATTGCATGGGTGGACCAAAATGTTTCCGCCTTCATATCTAAAAATGGGGTTGATATAAGGTATGTTGCTGTTGGTAATGAAGCTTTTCTAAAAACATACAATGGAAGATTTGTGCAATCAACATTTCCAGCCATAAAAAACATTCAAGCAGCCCTTATAAAAGCAGGGTTAGGCAGACAAGTAAAAGTAACAACCCCTTTAAATGCTGATGTCTACCAAAGTGACACTGGTCTTCCATCAGGTGGACACTTTAGACCAGACATTCAAAACCAAATGATGTCAATAATCAAATTTCTTAGCCAAAATAACGGCCCTCTTACATTTAACATCTACCCTTTTCTAAGCCTTGATGCTGATCCTAATTTCCCTAAAGAATTTGCTTTCTTTGATGGTTCTGCTGCCCCTGTAGTTGATGGTTCCATATCTTACACCAATGTTTTTGATGCAAATTTTGATACACTTATTTCAGCTCTTGAAAAAAATGGTTTTGGTTCAATGAATGTTATCATTGGTGAAGTTGGTTGGCCAACTGATGGAACATCAAATGCTAATATAAAAAGTGCTCAAAGATTTAATCAAGGACTTGTTGATAGAATAGTGAAAAAGCAAGGAACTCCTAAAAGACCTACCCCACCTGAAATTTACATGTTTGCCCTTTTAGATGAGGATTTAAAAAGTATTGATCCTGGCCCATTTGAGAGACATTGGGGAATTTTCAATTTTGATGGATCTATGAAATACCCTTTGAATCTTGGTGGTGGAAAATCACTAGTTGGTGCTAAAGGGGTTAAATATTTGCCAAAACAATGGTGTGTTATTTCATCTCAAGCAAATGTAATGGATCCTAATTTTGCACAAAGTATGAGCAAAGCTTGTACATACGCTGATTGTACATCTCTTGCTCCTGGCTCTTCTTGTAGTGGTTTAGACACAAAGGGTAATGCTTCATATGCATTTAATATGTATTATCAAAGAATGGATCAACGTAAAGATGCATGTCAATTCAATGGTTTATCAGTTGTTACCAACATTGATCCTTCACCTTCTCAAGGTAGTTGTCATTTTGAGATCATGATTGATATTGGGAAGCATGAAACTAAGTCCACTTCTTTTGCTGCACCAAAAATTGGATTAGAGTACATAATGGTCATGCTGGTATCAAGCTTCAGTTTCACCTTATCTCTATTTGTATAG
Protein sequence

>Msa1219820

MGFQRFSTCFLLALCILSQSLAKGTLGFACNWGTITSHPLPAQIMVKLMKDNGINKVKLFEADPMALKALGNSGIQVMVGIPNDLLDSLASNVNAAIAWVDQNVSAFISKNGVDIRYVAVGNEAFLKTYNGRFVQSTFPAIKNIQAALIKAGLGRQVKVTTPLNADVYQSDTGLPSGGHFRPDIQNQMMSIIKFLSQNNGPLTFNIYPFLSLDADPNFPKEFAFFDGSAAPVVDGSISYTNVFDANFDTLISALEKNGFGSMNVIIGEVGWPTDGTSNANIKSAQRFNQGLVDRIVKKQGTPKRPTPPEIYMFALLDEDLKSIDPGPFERHWGIFNFDGSMKYPLNLGGGKSLVGAKGVKYLPKQWCVISSQANVMDPNFAQSMSKACTYADCTSLAPGSSCSGLDTKGNASYAFNMYYQRMDQRKDACQFNGLSVVTNIDPSPSQGSCHFEIMIDIGKHETKSTSFAAPKIGLEYIMVMLVSSFSFTLSLFV*