AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421900 MtrunA17_Chr3g0116141 94.245 139 8 0 1 139 1 139 2.65e-92 263
Msa1421900 MtrunA17_Chr3g0116151 94.245 139 8 0 1 139 2 140 2.89e-92 263
Msa1421900 MtrunA17_Chr4g0057641 72.993 137 37 0 3 139 4 140 1.74e-68 203
Msa1421900 MtrunA17_Chr4g0057651 74.436 133 34 0 6 138 7 139 3.07e-67 200
Msa1421900 MtrunA17_Chr4g0057661 72.794 136 37 0 3 138 4 139 4.36e-67 199
Msa1421900 MtrunA17_Chr6g0459231 54.745 137 62 0 2 138 5 141 5.40e-51 159
Msa1421900 MtrunA17_Chr7g0225921 51.449 138 66 1 2 138 3 140 2.22e-45 144
Msa1421900 MtrunA17_Chr8g0373691 48.148 135 69 1 5 138 53 187 6.59e-35 119
Msa1421900 MtrunA17_Chr7g0245911 41.667 144 74 4 6 139 48 191 4.99e-28 102
Msa1421900 MtrunA17_Chr3g0111371 38.168 131 78 1 9 139 9 136 8.93e-28 99.8
Msa1421900 MtrunA17_Chr5g0434951 39.394 132 76 2 9 138 8 137 4.67e-27 98.2
Msa1421900 MtrunA17_Chr8g0392951 36.567 134 85 0 6 139 25 158 9.94e-27 98.2
Msa1421900 MtrunA17_Chr1g0161211 38.806 134 81 1 6 138 47 180 2.20e-26 98.2
Msa1421900 MtrunA17_Chr1g0169581 38.346 133 79 1 9 138 8 140 1.16e-25 94.7
Msa1421900 MtrunA17_Chr8g0351681 39.161 143 78 3 6 139 50 192 2.15e-25 95.5
Msa1421900 MtrunA17_Chr8g0391691 38.462 130 79 1 6 134 37 166 7.84e-25 94.0
Msa1421900 MtrunA17_Chr6g0482731 35.821 134 85 1 6 138 27 160 5.51e-22 86.3
Msa1421900 MtrunA17_Chr4g0033601 35.714 140 81 3 6 139 6 142 1.68e-21 84.3
Msa1421900 MtrunA17_Chr5g0402331 34.586 133 86 1 7 138 50 182 2.70e-21 84.7
Msa1421900 MtrunA17_Chr1g0169621 30.380 158 82 2 9 138 8 165 1.26e-20 82.4
Msa1421900 MtrunA17_Chr5g0402361 34.586 133 86 1 7 138 222 354 2.94e-20 84.7
Msa1421900 MtrunA17_Chr5g0402361 34.328 134 84 2 7 138 50 181 3.18e-18 79.3
Msa1421900 MtrunA17_Chr4g0044961 33.846 130 85 1 9 138 61 189 6.84e-20 81.3
Msa1421900 MtrunA17_Chr1g0188171 33.094 139 82 2 6 138 13 146 1.62e-19 79.0
Msa1421900 MtrunA17_Chr7g0254731 30.935 139 85 2 6 138 13 146 3.26e-19 78.2
Msa1421900 MtrunA17_Chr5g0440931 30.216 139 86 2 6 138 13 146 5.54e-18 75.1
Msa1421900 MtrunA17_Chr6g0460211 30.216 139 86 2 6 138 13 146 5.90e-18 75.1
Msa1421900 MtrunA17_Chr1g0166281 33.071 127 84 1 13 138 20 146 8.79e-18 74.7
Msa1421900 MtrunA17_Chr3g0093271 30.496 141 87 2 4 138 62 197 1.88e-17 75.1
Msa1421900 MtrunA17_Chr7g0256171 33.333 135 87 2 8 139 8 142 3.54e-17 73.2
Msa1421900 MtrunA17_Chr7g0256701 33.577 137 85 3 8 138 76 212 7.34e-17 73.6
Msa1421900 MtrunA17_Chr8g0366401 31.852 135 80 2 9 135 21 151 1.86e-16 71.6
Msa1421900 MtrunA17_Chr1g0185001 29.630 135 95 0 4 138 11 145 2.63e-16 70.9
Msa1421900 MtrunA17_Chr5g0396261 34.234 111 70 1 30 137 2 112 1.26e-15 68.2
Msa1421900 MtrunA17_Chr4g0062221 32.331 133 87 2 9 138 15 147 1.42e-15 69.3
Msa1421900 MtrunA17_Chr3g0121191 30.827 133 81 2 7 138 95 217 2.04e-15 70.1
Msa1421900 MtrunA17_Chr7g0245371 32.857 140 81 2 13 139 32 171 4.48e-15 68.2
Msa1421900 MtrunA17_Chr8g0368681 30.827 133 89 2 9 138 16 148 9.20e-15 67.0
Msa1421900 MtrunA17_Chr5g0397101 31.387 137 89 2 5 139 371 504 2.64e-13 65.9
Msa1421900 MtrunA17_Chr8g0387671 30.657 137 90 2 5 139 371 504 3.88e-13 65.1
Msa1421900 MtrunA17_Chr2g0329371 31.884 138 89 2 7 139 66 203 5.02e-13 63.5
Msa1421900 MtrunA17_Chr6g0450921 31.496 127 86 1 13 138 37 163 5.32e-13 62.8
Msa1421900 MtrunA17_Chr4g0064231 30.519 154 84 5 7 139 66 217 7.09e-13 63.2
Msa1421900 MtrunA17_Chr0c02g0489761 27.407 135 97 1 5 139 383 516 1.45e-12 63.5
Msa1421900 MtrunA17_Chr5g0409441 27.273 132 95 1 9 139 26 157 2.17e-12 60.8
Msa1421900 MtrunA17_Chr7g0245231 30.709 127 87 1 13 138 38 164 2.28e-12 60.8
Msa1421900 MtrunA17_Chr5g0441691 27.941 136 93 2 5 138 373 505 3.96e-12 62.4
Msa1421900 MtrunA17_Chr7g0274101 29.851 134 93 1 6 138 13 146 5.36e-12 59.7
Msa1421900 MtrunA17_Chr7g0242561 30.952 126 87 0 14 139 370 495 7.36e-12 61.6
Msa1421900 MtrunA17_Chr5g0444171 29.197 137 92 2 5 139 397 530 1.61e-11 60.5
Msa1421900 MtrunA17_Chr3g0131181 29.197 137 93 2 4 139 333 466 2.92e-11 59.7
Msa1421900 MtrunA17_Chr7g0257751 30.000 140 90 2 4 139 362 497 4.23e-11 59.3
Msa1421900 MtrunA17_Chr1g0173541 28.676 136 97 0 4 139 356 491 4.74e-11 59.3
Msa1421900 MtrunA17_Chr1g0157131 26.277 137 96 2 5 139 379 512 4.83e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421900 AT1G76650.1 47.101 138 73 0 2 139 40 177 5.05e-38 127
Msa1421900 AT1G76650.3 47.101 138 73 0 2 139 40 177 5.05e-38 127
Msa1421900 AT1G76640.1 46.853 143 69 3 2 139 19 159 2.28e-35 120
Msa1421900 AT1G76650.2 45.652 138 65 1 2 139 40 167 7.87e-35 119
Msa1421900 AT3G50770.1 45.985 137 68 2 8 138 67 203 2.80e-33 116
Msa1421900 AT5G42380.1 44.361 133 72 1 9 139 53 185 3.42e-33 115
Msa1421900 AT3G01830.1 40.146 137 77 2 6 139 12 146 5.16e-27 98.6
Msa1421900 AT1G24620.1 39.552 134 80 1 6 138 38 171 9.41e-27 99.0
Msa1421900 AT5G37770.1 35.252 139 85 1 6 139 18 156 2.01e-24 92.4
Msa1421900 AT5G37770.2 35.252 139 85 1 6 139 18 156 2.01e-24 92.4
Msa1421900 AT1G66400.1 33.088 136 88 1 6 138 16 151 2.71e-24 92.0
Msa1421900 AT1G18210.2 36.090 133 82 1 6 138 24 153 2.78e-23 89.7
Msa1421900 AT1G18210.1 36.090 133 82 1 6 138 24 153 2.78e-23 89.7
Msa1421900 AT1G73630.1 32.824 131 85 1 9 139 24 151 1.77e-21 84.7
Msa1421900 AT3G07490.1 35.211 142 89 2 1 139 1 142 6.08e-20 80.5
Msa1421900 AT1G05990.1 35.507 138 85 2 6 139 6 143 2.44e-19 79.0
Msa1421900 AT3G59440.1 34.074 135 87 1 7 139 53 187 6.41e-19 79.0
Msa1421900 AT2G15680.1 32.836 134 87 2 7 139 52 183 1.25e-18 78.2
Msa1421900 AT3G22930.1 32.090 134 90 1 6 138 36 169 1.54e-18 77.4
Msa1421900 AT4G03290.1 33.793 145 90 2 1 139 1 145 1.56e-18 77.0
Msa1421900 AT2G43290.1 32.394 142 88 2 6 139 66 207 1.88e-18 78.2
Msa1421900 AT5G17470.1 31.343 134 89 1 9 139 6 139 3.66e-18 75.9
Msa1421900 AT5G21274.1 30.935 139 85 2 6 138 13 146 3.89e-18 75.9
Msa1421900 AT3G56800.1 30.935 139 85 2 6 138 13 146 3.93e-18 75.9
Msa1421900 AT2G41110.1 30.935 139 85 2 6 138 13 146 3.93e-18 75.9
Msa1421900 AT2G27030.1 30.935 139 85 2 6 138 13 146 3.93e-18 75.9
Msa1421900 AT1G66410.1 30.216 139 86 2 6 138 13 146 4.42e-18 75.9
Msa1421900 AT5G37780.1 30.216 139 86 2 6 138 13 146 4.42e-18 75.9
Msa1421900 AT2G27030.3 30.935 139 85 2 6 138 13 146 7.83e-18 75.9
Msa1421900 AT3G43810.1 30.216 139 86 2 6 138 13 146 8.15e-18 75.1
Msa1421900 AT4G14640.1 31.343 134 91 1 6 138 14 147 2.01e-17 73.9
Msa1421900 AT3G10190.1 32.331 133 88 2 8 138 73 205 2.71e-17 75.1
Msa1421900 AT4G12860.1 34.074 135 86 2 8 139 8 142 3.70e-17 73.6
Msa1421900 AT3G43810.2 30.216 139 86 2 6 138 13 146 4.27e-17 74.7
Msa1421900 AT3G50360.1 32.812 128 85 1 13 139 35 162 5.44e-16 70.9
Msa1421900 AT2G41100.7 27.815 151 91 2 6 138 67 217 6.33e-16 72.8
Msa1421900 AT2G41100.6 27.815 151 91 2 6 138 67 217 6.33e-16 72.8
Msa1421900 AT2G41100.3 27.815 151 91 2 6 138 67 217 6.33e-16 72.8
Msa1421900 AT1G18530.1 32.331 133 87 1 9 138 11 143 6.99e-16 70.1
Msa1421900 AT2G41100.4 27.815 151 91 2 6 138 102 252 9.55e-16 72.4
Msa1421900 AT2G41100.4 25.333 150 95 2 6 138 13 162 1.21e-12 63.9
Msa1421900 AT2G41100.1 27.815 151 91 2 6 138 102 252 9.55e-16 72.4
Msa1421900 AT2G41100.1 25.333 150 95 2 6 138 13 162 1.21e-12 63.9
Msa1421900 AT2G41110.2 29.139 151 84 3 6 138 13 158 1.46e-15 69.3
Msa1421900 AT2G36180.1 28.889 135 92 2 9 139 4 138 3.24e-15 68.2
Msa1421900 AT3G03400.1 29.927 137 87 4 1 131 1 134 4.63e-15 67.8
Msa1421900 AT5G37780.2 27.273 154 86 3 6 138 13 161 6.26e-14 65.5
Msa1421900 AT4G23650.1 32.847 137 87 2 5 139 383 516 1.03e-13 67.4
Msa1421900 AT5G12180.1 30.370 135 93 1 5 139 378 511 2.51e-13 66.2
Msa1421900 AT5G19360.1 29.630 135 94 1 5 139 373 506 4.56e-13 65.5
Msa1421900 AT4G14640.2 29.752 121 84 1 19 138 9 129 4.62e-13 62.4
Msa1421900 AT5G37780.4 28.571 126 79 2 19 138 13 133 5.25e-13 62.4
Msa1421900 AT1G66410.2 28.571 126 79 2 19 138 36 156 6.60e-13 62.4
Msa1421900 AT3G51850.1 32.540 126 85 0 14 139 368 493 8.16e-13 64.7
Msa1421900 AT5G37780.3 28.571 126 79 2 19 138 52 172 8.62e-13 62.4
Msa1421900 AT1G32250.1 29.197 137 82 2 9 134 20 152 1.19e-12 62.0
Msa1421900 AT1G66410.4 32.075 106 59 3 41 138 10 110 1.74e-12 60.5
Msa1421900 AT1G66410.3 32.075 106 59 3 41 138 10 110 1.74e-12 60.5
Msa1421900 AT3G43810.4 32.075 106 59 3 41 138 10 110 2.84e-12 59.7
Msa1421900 AT3G43810.3 32.075 106 59 3 41 138 10 110 2.84e-12 59.7
Msa1421900 AT1G76040.1 29.197 137 92 2 5 139 178 311 5.02e-12 62.0
Msa1421900 AT1G76040.4 29.197 137 92 2 5 139 178 311 5.02e-12 62.0
Msa1421900 AT1G76040.2 29.197 137 92 2 5 139 416 549 5.27e-12 62.4
Msa1421900 AT3G25600.1 30.075 133 90 2 9 138 16 148 6.38e-12 60.1
Msa1421900 AT2G41090.1 26.316 133 96 1 6 138 13 143 9.33e-12 60.1
Msa1421900 AT4G37010.2 26.515 132 96 1 9 139 35 166 9.45e-12 59.7
Msa1421900 AT4G37010.1 26.515 132 96 1 9 139 31 162 9.60e-12 59.7
Msa1421900 AT2G41410.1 29.630 135 91 3 8 138 73 207 1.32e-11 60.1
Msa1421900 AT3G51920.1 26.866 134 97 1 6 138 13 146 1.37e-11 58.9
Msa1421900 AT2G31500.1 32.374 139 91 2 4 139 370 508 2.07e-11 60.8
Msa1421900 AT3G03000.1 29.104 134 93 1 4 135 19 152 3.70e-11 58.2
Msa1421900 AT3G10660.2 30.935 139 88 4 4 139 490 623 5.36e-11 59.3
Msa1421900 AT3G10660.1 30.935 139 88 4 4 139 490 623 5.36e-11 59.3
Msa1421900 AT1G12310.1 31.538 130 80 4 13 138 21 145 6.55e-11 57.0
Msa1421900 AT5G12480.2 28.467 137 97 1 4 139 269 405 8.03e-11 58.9
Msa1421900 AT1G62820.1 31.538 130 80 4 13 138 21 145 8.09e-11 56.6
Msa1421900 AT5G12480.1 28.467 137 97 1 4 139 363 499 8.59e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATCCTCTTCAAATTCTT+TGG 0.258063 tig0025315:-59728 None:intergenic
GGAGGAATTAATTGCTTTGA+TGG 0.320451 tig0025315:+59622 Msa1421900:CDS
TTTGTTGAAAGAAGCTGAAA+TGG 0.376240 tig0025315:+59556 Msa1421900:CDS
TGGTGATGGATATTTGAGTT+TGG 0.381496 tig0025315:+59601 Msa1421900:CDS
AGACAAAGGCTGGGAGTAAT+GGG 0.401953 tig0025315:+59524 Msa1421900:CDS
AGCCTTTGTCTTAGCTCTGC+TGG 0.405548 tig0025315:-59512 None:intergenic
GAAGAGGATGCTTAAGAAGA+TGG 0.422617 tig0025315:+59739 Msa1421900:CDS
AGCAGAGCTAAGACAAAGGC+TGG 0.426380 tig0025315:+59514 Msa1421900:CDS
TTAATTGCTTTGATGGAGGA+AGG 0.458662 tig0025315:+59629 Msa1421900:CDS
AAGACAAAGGCTGGGAGTAA+TGG 0.460108 tig0025315:+59523 Msa1421900:CDS
AGAAGCTGAAATGGCTATTG+AGG 0.481748 tig0025315:+59565 Msa1421900:CDS
AAGAGGATGCTTAAGAAGAT+GGG 0.484014 tig0025315:+59740 Msa1421900:CDS
TGGAGAGGAACAAAAGTTGA+AGG 0.498848 tig0025315:+59652 Msa1421900:CDS
ATTGCTTTGATGGAGGAAGG+TGG 0.541567 tig0025315:+59632 Msa1421900:CDS
TAACACCAAAGAATTTGAAG+AGG 0.563578 tig0025315:+59723 Msa1421900:CDS
TGATGGATATTTGAGTTTGG+AGG 0.566788 tig0025315:+59604 Msa1421900:CDS
GGAATTAATTGCTTTGATGG+AGG 0.567051 tig0025315:+59625 Msa1421900:CDS
TGAAATGGCTATTGAGGCAA+TGG 0.572267 tig0025315:+59571 Msa1421900:CDS
TGATGAAGATGGTGATGGAA+AGG 0.579290 tig0025315:+59484 Msa1421900:CDS
CACCAGCAGAGCTAAGACAA+AGG 0.624826 tig0025315:+59510 Msa1421900:CDS
GCAGAGCTAAGACAAAGGCT+GGG 0.627677 tig0025315:+59515 Msa1421900:CDS
TTTGATGGAGGAAGGTGGAG+AGG 0.629126 tig0025315:+59637 Msa1421900:CDS
ATTGAGGCAATGGATTCAGA+TGG 0.638749 tig0025315:+59581 Msa1421900:CDS
ATGTATGATAGTGAGAAGTG+TGG 0.644233 tig0025315:+59695 Msa1421900:CDS
GCAATGGATTCAGATGGTGA+TGG 0.644671 tig0025315:+59587 Msa1421900:CDS
TTATAAAAGATGAAGAACGC+GGG 0.655567 tig0025315:+59437 None:intergenic
ATTATAAAAGATGAAGAACG+CGG 0.665781 tig0025315:+59436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAACATTTTGATTTGAA+TGG + tig0025315:59797-59816 Msa1421900:CDS 15.0%
!!! TTAAACATTTTGATTTGAAT+GGG + tig0025315:59798-59817 Msa1421900:CDS 15.0%
!!! TAAACATTTTGATTTGAATG+GGG + tig0025315:59799-59818 Msa1421900:CDS 20.0%
!!! CTTAGATATTTTGATGAAGA+TGG + tig0025315:59473-59492 Msa1421900:CDS 25.0%
!!! TATTTTGATGAAGATGGTGA+TGG + tig0025315:59479-59498 Msa1421900:CDS 30.0%
TTTGTTGAAAGAAGCTGAAA+TGG + tig0025315:59556-59575 Msa1421900:CDS 30.0%
TAACACCAAAGAATTTGAAG+AGG + tig0025315:59723-59742 Msa1421900:CDS 30.0%
!! TGGTGATGGATATTTGAGTT+TGG + tig0025315:59601-59620 Msa1421900:CDS 35.0%
! TGATGGATATTTGAGTTTGG+AGG + tig0025315:59604-59623 Msa1421900:CDS 35.0%
GGAGGAATTAATTGCTTTGA+TGG + tig0025315:59622-59641 Msa1421900:CDS 35.0%
GGAATTAATTGCTTTGATGG+AGG + tig0025315:59625-59644 Msa1421900:CDS 35.0%
TTAATTGCTTTGATGGAGGA+AGG + tig0025315:59629-59648 Msa1421900:CDS 35.0%
! ATGTATGATAGTGAGAAGTG+TGG + tig0025315:59695-59714 Msa1421900:CDS 35.0%
AGCATCCTCTTCAAATTCTT+TGG - tig0025315:59731-59750 None:intergenic 35.0%
AAGAGGATGCTTAAGAAGAT+GGG + tig0025315:59740-59759 Msa1421900:CDS 35.0%
!!! CATTTTGATTTGAATGGGGA+TGG + tig0025315:59803-59822 Msa1421900:CDS 35.0%
!! TGATGAAGATGGTGATGGAA+AGG + tig0025315:59484-59503 Msa1421900:CDS 40.0%
AGAAGCTGAAATGGCTATTG+AGG + tig0025315:59565-59584 Msa1421900:CDS 40.0%
TGAAATGGCTATTGAGGCAA+TGG + tig0025315:59571-59590 Msa1421900:CDS 40.0%
! ATTGAGGCAATGGATTCAGA+TGG + tig0025315:59581-59600 Msa1421900:CDS 40.0%
TGGAGAGGAACAAAAGTTGA+AGG + tig0025315:59652-59671 Msa1421900:CDS 40.0%
GAAGAGGATGCTTAAGAAGA+TGG + tig0025315:59739-59758 Msa1421900:CDS 40.0%
AAGACAAAGGCTGGGAGTAA+TGG + tig0025315:59523-59542 Msa1421900:CDS 45.0%
AGACAAAGGCTGGGAGTAAT+GGG + tig0025315:59524-59543 Msa1421900:CDS 45.0%
! GCAATGGATTCAGATGGTGA+TGG + tig0025315:59587-59606 Msa1421900:CDS 45.0%
ATTGCTTTGATGGAGGAAGG+TGG + tig0025315:59632-59651 Msa1421900:CDS 45.0%
CACCAGCAGAGCTAAGACAA+AGG + tig0025315:59510-59529 Msa1421900:CDS 50.0%
!! AGCCTTTGTCTTAGCTCTGC+TGG - tig0025315:59515-59534 None:intergenic 50.0%
AGCAGAGCTAAGACAAAGGC+TGG + tig0025315:59514-59533 Msa1421900:CDS 50.0%
GCAGAGCTAAGACAAAGGCT+GGG + tig0025315:59515-59534 Msa1421900:CDS 50.0%
TTTGATGGAGGAAGGTGGAG+AGG + tig0025315:59637-59656 Msa1421900:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0025315 gene 59446 59865 59446 ID=Msa1421900;Name=Msa1421900
tig0025315 mRNA 59446 59865 59446 ID=Msa1421900-mRNA-1;Parent=Msa1421900;Name=Msa1421900-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|139
tig0025315 exon 59446 59865 59446 ID=Msa1421900-mRNA-1:exon:2299;Parent=Msa1421900-mRNA-1
tig0025315 CDS 59446 59865 59446 ID=Msa1421900-mRNA-1:cds;Parent=Msa1421900-mRNA-1
Gene Sequence

>Msa1421900

ATGAAGAACGCGGGATTCGAGCATGTTCTTAGATATTTTGATGAAGATGGTGATGGAAAGGTTTCACCAGCAGAGCTAAGACAAAGGCTGGGAGTAATGGGAGAAGAGATTTTGTTGAAAGAAGCTGAAATGGCTATTGAGGCAATGGATTCAGATGGTGATGGATATTTGAGTTTGGAGGAATTAATTGCTTTGATGGAGGAAGGTGGAGAGGAACAAAAGTTGAAGGATTTGAGAGATGCATTTGAGATGTATGATAGTGAGAAGTGTGGATTTATAACACCAAAGAATTTGAAGAGGATGCTTAAGAAGATGGGTGAGTCTAAGTCTATTGATGAATGTAAAGCTATGATTAAACATTTTGATTTGAATGGGGATGGTGTGCTTAGCTTTGATGAATTCACAGTAATGATGCAGTGA

Protein sequence

>Msa1421900

MKNAGFEHVLRYFDEDGDGKVSPAELRQRLGVMGEEILLKEAEMAIEAMDSDGDGYLSLEELIALMEEGGEEQKLKDLRDAFEMYDSEKCGFITPKNLKRMLKKMGESKSIDECKAMIKHFDLNGDGVLSFDEFTVMMQ*