AlfalfaGEDB | Msa0358110

M. sativa cultivar zhongmu-4 / Msa0358110

KEGG: K13448
GO: GO:0000302 GO:0001101 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010193 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0046872 GO:0050896 GO:1901700
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0358110	XP_024635986.2	97.122	139	4	0	1	139	1	139	4.59e-90	268

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0358110	sp\|Q9SRE6\|CML38_ARATH	46.377	138	74	0	2	139	40	177	4.77e-36	125

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0358110	G7J5J2	97.122	139	4	0	1	139	1	139	2.19e-90	268

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004480	Msa0358110	0.818799	1.623525e-52	-8.615850e-47
Msa0030480	Msa0358110	0.816106	6.559759e-52	-8.615850e-47
Msa0071480	Msa0358110	0.859150	5.045558e-63	-8.615850e-47
Msa0081940	Msa0358110	0.847508	1.106209e-59	-8.615850e-47
Msa0082050	Msa0358110	0.825308	5.031139e-54	-8.615850e-47
Msa0119440	Msa0358110	0.873542	1.356685e-67	-8.615850e-47
Msa0128430	Msa0358110	0.853502	2.289959e-61	-8.615850e-47
Msa0136630	Msa0358110	0.808024	3.795336e-50	-8.615850e-47
Msa0162690	Msa0358110	0.823867	1.099341e-53	-8.615850e-47
Msa0213430	Msa0358110	0.846835	1.691557e-59	-8.615850e-47
Msa0246590	Msa0358110	0.800494	1.404079e-48	-8.615850e-47
Msa0259440	Msa0358110	0.840302	9.435483e-58	-8.615850e-47
Msa0259580	Msa0358110	0.845281	4.478352e-59	-8.615850e-47
Msa0284240	Msa0358110	0.834971	2.201091e-56	-8.615850e-47
Msa0285070	Msa0358110	0.814539	1.463609e-51	-8.615850e-47
Msa0305620	Msa0358110	0.859292	4.574134e-63	-8.615850e-47
Msa0358110	Msa0358120	0.892976	9.657582e-75	-8.615850e-47
Msa0358110	Msa0364790	0.814311	1.643084e-51	-8.615850e-47
Msa0358110	Msa0380600	0.817537	3.132451e-52	-8.615850e-47
Msa0358110	Msa0418400	0.953795	1.244233e-111	-8.615850e-47
Msa0358110	Msa0418410	0.848166	7.288045e-60	-8.615850e-47
Msa0358110	Msa0430240	0.815345	9.696694e-52	-8.615850e-47
Msa0358110	Msa0437070	0.846411	2.208930e-59	-8.615850e-47
Msa0358110	Msa0472710	0.995497	7.396169e-217	-8.615850e-47
Msa0358110	Msa0472720	0.891376	4.202611e-74	-8.615850e-47
Msa0358110	Msa0472760	0.863001	3.397708e-64	-8.615850e-47
Msa0358110	Msa0472770	0.941810	2.172894e-101	-8.615850e-47
Msa0358110	Msa0482940	0.802681	4.999389e-49	-8.615850e-47
Msa0358110	Msa0583670	0.843645	1.233787e-58	-8.615850e-47
Msa0358110	Msa0615680	0.800077	1.706452e-48	-8.615850e-47
Msa0358110	Msa0659790	0.828226	1.011493e-54	-8.615850e-47
Msa0358110	Msa0724370	0.835653	1.480488e-56	-8.615850e-47
Msa0358110	Msa0735550	0.812592	3.923255e-51	-8.615850e-47
Msa0358110	Msa0748660	0.894526	2.270590e-75	-8.615850e-47
Msa0358110	Msa0764920	0.861350	1.091663e-63	-8.615850e-47
Msa0358110	Msa0769720	0.802760	4.815667e-49	-8.615850e-47
Msa0358110	Msa0775560	0.808249	3.397594e-50	-8.615850e-47
Msa0358110	Msa0775920	0.840149	1.034648e-57	-8.615850e-47
Msa0358110	Msa0813260	0.862918	3.605071e-64	-8.615850e-47
Msa0358110	Msa0844610	0.841896	3.598533e-58	-8.615850e-47
Msa0358110	Msa0853440	0.801035	1.088717e-48	-8.615850e-47
Msa0358110	Msa0853810	0.844733	6.296984e-59	-8.615850e-47
Msa0358110	Msa0855110	0.819014	1.450431e-52	-8.615850e-47
Msa0358110	Msa0860250	0.822779	1.973941e-53	-8.615850e-47
Msa0358110	Msa0899400	0.808153	3.561353e-50	-8.615850e-47
Msa0358110	Msa0968050	0.832967	6.987014e-56	-8.615850e-47
Msa0358110	Msa0973070	0.812492	4.126006e-51	-8.615850e-47
Msa0358110	Msa1034700	0.805303	1.424925e-49	-8.615850e-47
Msa0358110	Msa1074540	0.836706	7.994579e-57	-8.615850e-47
Msa0358110	Msa1078330	0.812087	5.057602e-51	-8.615850e-47
Msa0358110	Msa1108720	0.819831	9.448403e-53	-8.615850e-47
Msa0358110	Msa1123720	0.833183	6.173472e-56	-8.615850e-47
Msa0358110	Msa1163880	0.812437	4.241408e-51	-8.615850e-47
Msa0358110	Msa1238680	0.811631	6.354866e-51	-8.615850e-47
Msa0358110	Msa1258670	0.804316	2.290750e-49	-8.615850e-47
Msa0358110	Msa1277780	0.800751	1.244088e-48	-8.615850e-47
Msa0358110	Msa1285200	0.841115	5.777560e-58	-8.615850e-47
Msa0358110	Msa1361250	0.853433	2.397269e-61	-8.615850e-47
Msa0358110	Msa1370590	0.804999	1.649725e-49	-8.615850e-47
Msa0358110	Msa1384100	0.805098	1.572439e-49	-8.615850e-47
Msa0358110	Msa1389760	0.812005	5.271074e-51	-8.615850e-47
Msa0358110	Msa1421890	0.802538	5.348846e-49	-8.615850e-47
Msa0358110	Msa1421900	0.955412	3.219772e-113	-8.615850e-47
Msa0358110	Msa1422810	0.803619	3.197002e-49	-8.615850e-47
Msa0358110	Msa1444120	0.802891	4.522816e-49	-8.615850e-47
Msa0358110	Msa1462470	0.819055	1.419516e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0358110	MtrunA17_Chr3g0116141	97.122	139	4	0	1	139	1	139	4.22e-94	268
Msa0358110	MtrunA17_Chr3g0116151	97.122	139	4	0	1	139	2	140	5.32e-94	267
Msa0358110	MtrunA17_Chr4g0057641	74.453	137	35	0	3	139	4	140	2.24e-69	205
Msa0358110	MtrunA17_Chr4g0057651	75.940	133	32	0	6	138	7	139	2.72e-68	202
Msa0358110	MtrunA17_Chr4g0057661	74.265	136	35	0	3	138	4	139	3.78e-68	202
Msa0358110	MtrunA17_Chr6g0459231	56.204	137	60	0	2	138	5	141	2.52e-52	162
Msa0358110	MtrunA17_Chr7g0225921	53.623	138	63	1	2	138	3	140	4.20e-47	149
Msa0358110	MtrunA17_Chr8g0373691	48.148	135	69	1	5	138	53	187	1.85e-35	120
Msa0358110	MtrunA17_Chr3g0111371	39.695	131	76	1	9	139	9	136	3.95e-29	103
Msa0358110	MtrunA17_Chr8g0392951	38.931	131	80	0	9	139	28	158	3.33e-28	101
Msa0358110	MtrunA17_Chr7g0245911	40.278	144	76	4	6	139	48	191	3.70e-27	100
Msa0358110	MtrunA17_Chr5g0434951	39.394	132	76	2	9	138	8	137	3.85e-27	98.2
Msa0358110	MtrunA17_Chr1g0161211	38.806	134	81	1	6	138	47	180	9.72e-27	99.0
Msa0358110	MtrunA17_Chr1g0169581	38.346	133	79	1	9	138	8	140	7.54e-26	95.1
Msa0358110	MtrunA17_Chr8g0391691	38.462	130	79	1	6	134	37	166	8.73e-25	93.6
Msa0358110	MtrunA17_Chr8g0351681	37.762	143	80	3	6	139	50	192	1.74e-24	93.2
Msa0358110	MtrunA17_Chr4g0033601	37.143	140	79	3	6	139	6	142	3.76e-23	88.6
Msa0358110	MtrunA17_Chr5g0402331	36.090	133	84	1	7	138	50	182	1.41e-22	88.2
Msa0358110	MtrunA17_Chr6g0482731	36.641	131	82	1	9	138	30	160	5.33e-22	86.3
Msa0358110	MtrunA17_Chr5g0402361	36.090	133	84	1	7	138	222	354	1.92e-21	88.2
Msa0358110	MtrunA17_Chr5g0402361	35.821	134	82	2	7	138	50	181	1.72e-19	82.8
Msa0358110	MtrunA17_Chr4g0044961	34.615	130	84	1	9	138	61	189	5.03e-21	84.3
Msa0358110	MtrunA17_Chr1g0169621	30.380	158	82	2	9	138	8	165	8.49e-21	82.8
Msa0358110	MtrunA17_Chr1g0188171	34.532	139	80	2	6	138	13	146	8.62e-21	82.4
Msa0358110	MtrunA17_Chr7g0254731	32.374	139	83	2	6	138	13	146	1.09e-20	82.0
Msa0358110	MtrunA17_Chr5g0440931	31.655	139	84	2	6	138	13	146	2.19e-19	78.6
Msa0358110	MtrunA17_Chr6g0460211	31.655	139	84	2	6	138	13	146	2.24e-19	78.6
Msa0358110	MtrunA17_Chr1g0166281	34.646	127	82	1	13	138	20	146	3.37e-19	78.2
Msa0358110	MtrunA17_Chr3g0093271	31.915	141	85	2	4	138	62	197	7.27e-19	78.6
Msa0358110	MtrunA17_Chr7g0256171	34.815	135	85	2	8	139	8	142	2.26e-18	76.3
Msa0358110	MtrunA17_Chr8g0366401	32.836	134	78	2	9	134	21	150	8.72e-18	75.1
Msa0358110	MtrunA17_Chr7g0256701	33.577	137	85	3	8	138	76	212	7.58e-17	73.6
Msa0358110	MtrunA17_Chr1g0185001	29.630	135	95	0	4	138	11	145	2.50e-16	70.9
Msa0358110	MtrunA17_Chr3g0121191	31.579	133	80	2	7	138	95	217	4.29e-16	72.0
Msa0358110	MtrunA17_Chr4g0062221	32.331	133	87	2	9	138	15	147	8.83e-16	69.7
Msa0358110	MtrunA17_Chr5g0396261	34.234	111	70	1	30	137	2	112	8.95e-16	68.6
Msa0358110	MtrunA17_Chr7g0245371	32.857	140	81	2	13	139	32	171	4.53e-15	68.2
Msa0358110	MtrunA17_Chr2g0329371	34.058	138	86	2	7	139	66	203	1.92e-14	67.0
Msa0358110	MtrunA17_Chr8g0368681	30.075	133	90	2	9	138	16	148	2.31e-14	65.9
Msa0358110	MtrunA17_Chr5g0397101	32.117	137	88	2	5	139	371	504	2.59e-14	68.6
Msa0358110	MtrunA17_Chr4g0064231	31.818	154	82	5	7	139	66	217	3.02e-14	67.0
Msa0358110	MtrunA17_Chr8g0387671	31.387	137	89	2	5	139	371	504	4.41e-14	67.8
Msa0358110	MtrunA17_Chr0c02g0489761	28.148	135	96	1	5	139	383	516	2.32e-13	65.9
Msa0358110	MtrunA17_Chr5g0409441	28.030	132	94	1	9	139	26	157	3.59e-13	62.8
Msa0358110	MtrunA17_Chr7g0274101	32.090	134	90	1	6	138	13	146	3.73e-13	62.4
Msa0358110	MtrunA17_Chr5g0441691	28.676	136	92	2	5	138	373	505	5.41e-13	64.7
Msa0358110	MtrunA17_Chr7g0242561	31.746	126	86	0	14	139	370	495	6.52e-13	64.7
Msa0358110	MtrunA17_Chr5g0444171	29.927	137	91	2	5	139	397	530	2.19e-12	63.2
Msa0358110	MtrunA17_Chr6g0450921	30.469	128	88	1	13	139	37	164	2.94e-12	60.5
Msa0358110	MtrunA17_Chr7g0257751	30.714	140	89	2	4	139	362	497	4.59e-12	62.0
Msa0358110	MtrunA17_Chr1g0157131	27.007	137	95	2	5	139	379	512	4.67e-12	62.0
Msa0358110	MtrunA17_Chr1g0173541	29.412	136	96	0	4	139	356	491	5.72e-12	62.0
Msa0358110	MtrunA17_Chr3g0131181	29.927	137	92	2	4	139	333	466	6.53e-12	61.6
Msa0358110	MtrunA17_Chr4g0033371	31.159	138	88	3	5	139	377	510	8.55e-12	61.2
Msa0358110	MtrunA17_Chr7g0245231	29.688	128	89	1	13	139	38	165	1.34e-11	58.9
Msa0358110	MtrunA17_Chr3g0098951	28.369	141	95	2	5	139	388	528	2.24e-11	60.1
Msa0358110	MtrunA17_Chr4g0074811	28.467	137	93	2	5	139	396	529	3.60e-11	59.7
Msa0358110	MtrunA17_Chr8g0385341	29.412	136	92	2	5	139	335	467	9.69e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0358110	AT1G76650.1	46.377	138	74	0	2	139	40	177	4.85e-37	125
Msa0358110	AT1G76650.3	46.377	138	74	0	2	139	40	177	4.85e-37	125
Msa0358110	AT1G76640.1	46.853	143	69	3	2	139	19	159	4.20e-35	119
Msa0358110	AT1G76650.2	44.928	138	66	1	2	139	40	167	7.54e-34	116
Msa0358110	AT3G50770.1	46.715	137	67	2	8	138	67	203	7.66e-34	117
Msa0358110	AT5G42380.1	44.361	133	72	1	9	139	53	185	6.55e-33	114
Msa0358110	AT3G01830.1	40.876	137	76	2	6	139	12	146	4.20e-27	99.0
Msa0358110	AT1G24620.1	39.552	134	80	1	6	138	38	171	1.06e-26	99.0
Msa0358110	AT1G66400.1	34.559	136	86	1	6	138	16	151	7.65e-26	95.9
Msa0358110	AT5G37770.1	36.691	139	83	1	6	139	18	156	8.12e-26	95.9
Msa0358110	AT5G37770.2	36.691	139	83	1	6	139	18	156	8.12e-26	95.9
Msa0358110	AT1G18210.2	36.923	130	79	1	9	138	27	153	2.15e-23	90.1
Msa0358110	AT1G18210.1	36.923	130	79	1	9	138	27	153	2.15e-23	90.1
Msa0358110	AT1G73630.1	34.351	131	83	1	9	139	24	151	4.82e-23	89.0
Msa0358110	AT3G07490.1	36.620	142	87	2	1	139	1	142	3.75e-21	83.6
Msa0358110	AT1G05990.1	36.957	138	83	2	6	139	6	143	7.25e-21	82.8
Msa0358110	AT4G03290.1	35.374	147	85	4	1	139	1	145	5.36e-20	80.9
Msa0358110	AT3G22930.1	33.582	134	88	1	6	138	36	169	6.78e-20	80.9
Msa0358110	AT5G17470.1	32.836	134	87	1	9	139	6	139	1.01e-19	79.7
Msa0358110	AT2G15680.1	34.328	134	85	2	7	139	52	183	1.03e-19	80.9
Msa0358110	AT3G56800.1	32.374	139	83	2	6	138	13	146	1.38e-19	79.7
Msa0358110	AT2G41110.1	32.374	139	83	2	6	138	13	146	1.38e-19	79.7
Msa0358110	AT2G27030.1	32.374	139	83	2	6	138	13	146	1.38e-19	79.7
Msa0358110	AT5G21274.1	32.374	139	83	2	6	138	13	146	1.49e-19	79.3
Msa0358110	AT1G66410.1	31.655	139	84	2	6	138	13	146	1.69e-19	79.3
Msa0358110	AT5G37780.1	31.655	139	84	2	6	138	13	146	1.69e-19	79.3
Msa0358110	AT2G27030.3	32.374	139	83	2	6	138	13	146	2.82e-19	79.7
Msa0358110	AT3G43810.1	31.655	139	84	2	6	138	13	146	3.09e-19	78.6
Msa0358110	AT3G59440.1	34.074	135	87	1	7	139	53	187	3.65e-19	79.7
Msa0358110	AT2G43290.1	32.394	142	88	2	6	139	66	207	9.09e-19	79.0
Msa0358110	AT4G14640.1	32.836	134	89	1	6	138	14	147	9.89e-19	77.4
Msa0358110	AT3G10190.1	33.835	133	86	2	8	138	73	205	1.22e-18	78.6
Msa0358110	AT3G43810.2	31.655	139	84	2	6	138	13	146	1.61e-18	78.6
Msa0358110	AT4G12860.1	35.556	135	84	2	8	139	8	142	2.29e-18	76.6
Msa0358110	AT2G41100.7	29.139	151	89	2	6	138	67	217	3.62e-17	75.9
Msa0358110	AT2G41100.6	29.139	151	89	2	6	138	67	217	3.62e-17	75.9
Msa0358110	AT2G41100.3	29.139	151	89	2	6	138	67	217	3.62e-17	75.9
Msa0358110	AT2G41100.4	29.139	151	89	2	6	138	102	252	5.63e-17	75.9
Msa0358110	AT2G41100.4	26.667	150	93	2	6	138	13	162	9.28e-14	67.0
Msa0358110	AT2G41100.1	29.139	151	89	2	6	138	102	252	5.63e-17	75.9
Msa0358110	AT2G41100.1	26.667	150	93	2	6	138	13	162	9.28e-14	67.0
Msa0358110	AT2G41110.2	30.464	151	82	3	6	138	13	158	5.71e-17	73.2
Msa0358110	AT3G50360.1	33.594	128	84	1	13	139	35	162	9.31e-17	72.8
Msa0358110	AT2G36180.1	30.370	135	90	2	9	139	4	138	9.81e-17	72.0
Msa0358110	AT5G37780.2	28.571	154	84	3	6	138	13	161	2.17e-15	68.9
Msa0358110	AT1G18530.1	31.579	133	88	1	9	138	11	143	2.85e-15	68.6
Msa0358110	AT3G03400.1	29.927	137	87	4	1	131	1	134	2.86e-15	68.2
Msa0358110	AT5G37780.4	30.159	126	77	2	19	138	13	133	1.83e-14	65.9
Msa0358110	AT4G23650.1	33.577	137	86	2	5	139	383	516	1.92e-14	69.3
Msa0358110	AT4G14640.2	31.405	121	82	1	19	138	9	129	1.99e-14	65.9
Msa0358110	AT1G66410.2	30.159	126	77	2	19	138	36	156	2.23e-14	66.2
Msa0358110	AT5G37780.3	30.159	126	77	2	19	138	52	172	3.67e-14	66.2
Msa0358110	AT5G12180.1	31.111	135	92	1	5	139	378	511	4.04e-14	68.6
Msa0358110	AT1G66410.4	33.962	106	57	3	41	138	10	110	5.99e-14	63.9
Msa0358110	AT1G66410.3	33.962	106	57	3	41	138	10	110	5.99e-14	63.9
Msa0358110	AT5G19360.1	30.370	135	93	1	5	139	373	506	6.99e-14	67.8
Msa0358110	AT3G51850.1	33.333	126	84	0	14	139	368	493	8.18e-14	67.4
Msa0358110	AT3G43810.4	33.962	106	57	3	41	138	10	110	9.80e-14	63.5
Msa0358110	AT3G43810.3	33.962	106	57	3	41	138	10	110	9.80e-14	63.5
Msa0358110	AT1G32250.1	29.927	137	81	2	9	134	20	152	2.31e-13	63.9
Msa0358110	AT1G76040.1	30.657	137	90	2	5	139	178	311	2.78e-13	65.5
Msa0358110	AT1G76040.4	30.657	137	90	2	5	139	178	311	2.78e-13	65.5
Msa0358110	AT1G76040.2	30.657	137	90	2	5	139	416	549	2.90e-13	65.9
Msa0358110	AT3G51920.1	28.358	134	95	1	6	138	13	146	6.15e-13	62.4
Msa0358110	AT2G41090.1	27.068	133	95	1	6	138	13	143	8.26e-13	62.8
Msa0358110	AT4G37010.2	27.273	132	95	1	9	139	35	166	1.45e-12	62.0
Msa0358110	AT4G37010.1	27.273	132	95	1	9	139	31	162	1.45e-12	61.6
Msa0358110	AT3G03000.1	29.851	134	92	1	4	135	19	152	6.63e-12	60.1
Msa0358110	AT5G12480.2	29.197	137	96	1	4	139	269	405	6.97e-12	62.0
Msa0358110	AT5G12480.1	29.197	137	96	1	4	139	363	499	7.26e-12	62.0
Msa0358110	AT3G10660.2	31.655	139	87	4	4	139	490	623	8.39e-12	61.6
Msa0358110	AT3G10660.1	31.655	139	87	4	4	139	490	623	8.39e-12	61.6
Msa0358110	AT4G04695.1	28.148	135	96	1	5	139	336	469	1.40e-11	61.2
Msa0358110	AT1G50700.1	27.737	137	94	2	5	139	378	511	1.41e-11	61.2
Msa0358110	AT2G41410.1	29.630	135	91	3	8	138	73	207	1.43e-11	60.1
Msa0358110	AT1G74740.1	32.308	130	80	3	14	139	373	498	1.56e-11	60.8
Msa0358110	AT2G31500.1	32.374	139	91	2	4	139	370	508	1.86e-11	60.8
Msa0358110	AT2G41100.2	31.481	108	56	2	49	138	20	127	2.54e-11	58.9
Msa0358110	AT1G35670.1	29.412	136	92	2	5	139	331	463	2.58e-11	60.5
Msa0358110	AT4G09570.1	29.412	136	92	2	5	139	330	462	2.59e-11	60.5
Msa0358110	AT3G25600.1	29.323	133	91	2	9	138	16	148	2.66e-11	58.2
Msa0358110	AT3G20410.2	27.737	137	94	2	5	139	396	529	3.38e-11	60.1
Msa0358110	AT3G20410.1	27.737	137	94	2	5	139	396	529	3.38e-11	60.1
Msa0358110	AT4G21940.2	29.197	137	92	2	5	139	406	539	4.45e-11	59.7
Msa0358110	AT4G21940.1	29.197	137	92	2	5	139	406	539	4.66e-11	59.7
Msa0358110	AT3G01830.2	36.538	104	61	2	6	106	12	113	6.50e-11	56.6
Msa0358110	AT5G23580.1	28.676	136	93	2	5	139	327	459	9.77e-11	58.5

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCATCCTCTTCAAACTCTT+TGG	0.279285	3_1:+42844166	None:intergenic
GGAGGAATTAATTGCTTTGA+TGG	0.320451	3_1:-42844272	Msa0358110:CDS
TTTGTTGAAAGAAGCTGAAA+TGG	0.376240	3_1:-42844338	Msa0358110:CDS
TGGTGATGGATATTTGAGTT+TGG	0.381496	3_1:-42844293	Msa0358110:CDS
GAAGAGGATGCTTAAGAAGA+TGG	0.388089	3_1:-42844155	Msa0358110:CDS
AGACAAAGGCTGGGAGTAAT+GGG	0.401953	3_1:-42844370	Msa0358110:CDS
AGCCTTTGTCTTAGCTCTGC+TGG	0.405548	3_1:+42844382	None:intergenic
AGCAGAGCTAAGACAAAGGC+TGG	0.426380	3_1:-42844380	Msa0358110:CDS
TTAATTGCTTTGATGGAGGA+AGG	0.458662	3_1:-42844265	Msa0358110:CDS
AAGACAAAGGCTGGGAGTAA+TGG	0.460108	3_1:-42844371	Msa0358110:CDS
AGAAGCTGAAATGGCTATTG+AGG	0.481748	3_1:-42844329	Msa0358110:CDS
AAGAGGATGCTTAAGAAGAT+GGG	0.484014	3_1:-42844154	Msa0358110:CDS
TGGAGAGGAACAAAAGTTGA+AGG	0.498848	3_1:-42844242	Msa0358110:CDS
ATTGCTTTGATGGAGGAAGG+TGG	0.541567	3_1:-42844262	Msa0358110:CDS
TGATGGATATTTGAGTTTGG+AGG	0.566788	3_1:-42844290	Msa0358110:CDS
GGAATTAATTGCTTTGATGG+AGG	0.567051	3_1:-42844269	Msa0358110:CDS
TGAAATGGCTATTGAGGCAA+TGG	0.572267	3_1:-42844323	Msa0358110:CDS
TGATGAAGATGGTGATGGAA+AGG	0.579290	3_1:-42844410	Msa0358110:CDS
TAACACCAAAGAGTTTGAAG+AGG	0.591633	3_1:-42844171	Msa0358110:CDS
CACCAGCAGAGCTAAGACAA+AGG	0.624826	3_1:-42844384	Msa0358110:CDS
GCAGAGCTAAGACAAAGGCT+GGG	0.627677	3_1:-42844379	Msa0358110:CDS
TTTGATGGAGGAAGGTGGAG+AGG	0.629126	3_1:-42844257	Msa0358110:CDS
ATTGAGGCAATGGATTCAGA+TGG	0.638749	3_1:-42844313	Msa0358110:CDS
GCAATGGATTCAGATGGTGA+TGG	0.644671	3_1:-42844307	Msa0358110:CDS
TTATAAAAGATGAAGAACGC+GGG	0.655567	3_1:-42844457	None:intergenic
ATTATAAAAGATGAAGAACG+CGG	0.665781	3_1:-42844458	None:intergenic
ATGTATGATAGTGAGAAGTG+TGG	0.719192	3_1:-42844199	Msa0358110:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATGATTAAACATTTTGATT+TGG	-	chr3_1:42844398-42844417	Msa0358110:CDS	15.0%
!!!	ATTAAACATTTTGATTTGGA+TGG	-	chr3_1:42844402-42844421	Msa0358110:CDS	20.0%
!!!	TTAAACATTTTGATTTGGAT+GGG	-	chr3_1:42844403-42844422	Msa0358110:CDS	20.0%
!!!	CTTAGATATTTTGATGAAGA+TGG	-	chr3_1:42844078-42844097	Msa0358110:CDS	25.0%
!!!	TAAACATTTTGATTTGGATG+GGG	-	chr3_1:42844404-42844423	Msa0358110:CDS	25.0%
!!!	TATTTTGATGAAGATGGTGA+TGG	-	chr3_1:42844084-42844103	Msa0358110:CDS	30.0%
	TTTGTTGAAAGAAGCTGAAA+TGG	-	chr3_1:42844161-42844180	Msa0358110:CDS	30.0%
!!	TGGTGATGGATATTTGAGTT+TGG	-	chr3_1:42844206-42844225	Msa0358110:CDS	35.0%
!	TGATGGATATTTGAGTTTGG+AGG	-	chr3_1:42844209-42844228	Msa0358110:CDS	35.0%
	GGAGGAATTAATTGCTTTGA+TGG	-	chr3_1:42844227-42844246	Msa0358110:CDS	35.0%
	GGAATTAATTGCTTTGATGG+AGG	-	chr3_1:42844230-42844249	Msa0358110:CDS	35.0%
	TTAATTGCTTTGATGGAGGA+AGG	-	chr3_1:42844234-42844253	Msa0358110:CDS	35.0%
!	ATGTATGATAGTGAGAAGTG+TGG	-	chr3_1:42844300-42844319	Msa0358110:CDS	35.0%
	TAACACCAAAGAGTTTGAAG+AGG	-	chr3_1:42844328-42844347	Msa0358110:CDS	35.0%
	AAGAGGATGCTTAAGAAGAT+GGG	-	chr3_1:42844345-42844364	Msa0358110:CDS	35.0%
!!	TGATGAAGATGGTGATGGAA+AGG	-	chr3_1:42844089-42844108	Msa0358110:CDS	40.0%
	AGAAGCTGAAATGGCTATTG+AGG	-	chr3_1:42844170-42844189	Msa0358110:CDS	40.0%
	TGAAATGGCTATTGAGGCAA+TGG	-	chr3_1:42844176-42844195	Msa0358110:CDS	40.0%
!	ATTGAGGCAATGGATTCAGA+TGG	-	chr3_1:42844186-42844205	Msa0358110:CDS	40.0%
	TGGAGAGGAACAAAAGTTGA+AGG	-	chr3_1:42844257-42844276	Msa0358110:CDS	40.0%
	AGCATCCTCTTCAAACTCTT+TGG	+	chr3_1:42844336-42844355	None:intergenic	40.0%
	GAAGAGGATGCTTAAGAAGA+TGG	-	chr3_1:42844344-42844363	Msa0358110:CDS	40.0%
!!!	CATTTTGATTTGGATGGGGA+TGG	-	chr3_1:42844408-42844427	Msa0358110:CDS	40.0%
	AAGACAAAGGCTGGGAGTAA+TGG	-	chr3_1:42844128-42844147	Msa0358110:CDS	45.0%
	AGACAAAGGCTGGGAGTAAT+GGG	-	chr3_1:42844129-42844148	Msa0358110:CDS	45.0%
!	GCAATGGATTCAGATGGTGA+TGG	-	chr3_1:42844192-42844211	Msa0358110:CDS	45.0%
	ATTGCTTTGATGGAGGAAGG+TGG	-	chr3_1:42844237-42844256	Msa0358110:CDS	45.0%
	CACCAGCAGAGCTAAGACAA+AGG	-	chr3_1:42844115-42844134	Msa0358110:CDS	50.0%
!!	AGCCTTTGTCTTAGCTCTGC+TGG	+	chr3_1:42844120-42844139	None:intergenic	50.0%
	AGCAGAGCTAAGACAAAGGC+TGG	-	chr3_1:42844119-42844138	Msa0358110:CDS	50.0%
	GCAGAGCTAAGACAAAGGCT+GGG	-	chr3_1:42844120-42844139	Msa0358110:CDS	50.0%
	TTTGATGGAGGAAGGTGGAG+AGG	-	chr3_1:42844242-42844261	Msa0358110:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	42844051	42844470	42844051	ID=Msa0358110;Name=Msa0358110
chr3_1	mRNA	42844051	42844470	42844051	ID=Msa0358110-mRNA-1;Parent=Msa0358110;Name=Msa0358110-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|139
chr3_1	exon	42844051	42844470	42844051	ID=Msa0358110-mRNA-1:exon:7425;Parent=Msa0358110-mRNA-1
chr3_1	CDS	42844051	42844470	42844051	ID=Msa0358110-mRNA-1:cds;Parent=Msa0358110-mRNA-1

Gene Sequence

>Msa0358110

ATGAAGAACGCGGGATTCGAGCATGTTCTTAGATATTTTGATGAAGATGGTGATGGAAAGGTTTCACCAGCAGAGCTAAGACAAAGGCTGGGAGTAATGGGAGAAGAGATTTTGTTGAAAGAAGCTGAAATGGCTATTGAGGCAATGGATTCAGATGGTGATGGATATTTGAGTTTGGAGGAATTAATTGCTTTGATGGAGGAAGGTGGAGAGGAACAAAAGTTGAAGGATTTGAGAGAAGCATTTGAGATGTATGATAGTGAGAAGTGTGGTTTTATAACACCAAAGAGTTTGAAGAGGATGCTTAAGAAGATGGGTGAGTCTAAGTCTATTGATGAATGTAAAGCTATGATTAAACATTTTGATTTGGATGGGGATGGTTTGCTTAGCTTTGATGAATTCACAGTAATGATGCAGTGA

Protein sequence

>Msa0358110

MKNAGFEHVLRYFDEDGDGKVSPAELRQRLGVMGEEILLKEAEMAIEAMDSDGDGYLSLEELIALMEEGGEEQKLKDLREAFEMYDSEKCGFITPKSLKRMLKKMGESKSIDECKAMIKHFDLDGDGLLSFDEFTVMMQ*