AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1262860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262860 MtrunA17_Chr4g0065951 98.765 162 2 0 1 162 1 162 1.62e-113 318
Msa1262860 MtrunA17_Chr2g0303931 50.340 147 73 0 1 147 1 147 2.53e-40 133
Msa1262860 MtrunA17_Chr6g0478031 47.020 151 80 0 7 157 2 152 2.70e-40 133
Msa1262860 MtrunA17_Chr1g0199041 38.926 149 67 4 13 155 12 142 5.84e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262860 AT5G18970.1 69.620 158 48 0 1 158 1 158 3.36e-79 233
Msa1262860 AT5G46530.1 46.939 147 78 0 7 153 7 153 3.32e-41 135
Msa1262860 AT1G29520.1 49.020 153 78 0 1 153 1 153 2.90e-39 131
Msa1262860 AT1G04560.1 39.490 157 81 2 1 156 1 144 5.86e-26 97.8

Find 52 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCCATTGGAAAGTATAT+TGG 0.242510 8_2:+65776873 None:intergenic
TTCTTCTGAGAATTCATATA+TGG 0.274907 8_2:+65776128 Msa1262850:intron
ACACTAGATTGAGGGTTAAA+AGG 0.282593 8_2:+65777347 None:intergenic
ATGGCTGCAGCATTTAAATT+AGG 0.305394 8_2:+65776750 None:intergenic
ATAATCTTCACTCTAATTGC+TGG 0.330951 8_2:-65776831 Msa1262860:CDS
ACTCTAATTGCTGGTGTTGT+GGG 0.351945 8_2:-65776822 Msa1262860:CDS
GGTTTCACTACATCAGTAAC+TGG 0.354086 8_2:-65776801 Msa1262860:CDS
TCCCCATTGGAAAGTATATT+GGG 0.354392 8_2:+65776874 None:intergenic
CACTCTAATTGCTGGTGTTG+TGG 0.382264 8_2:-65776823 Msa1262860:CDS
GCACGACAGTTATCCATGTT+GGG 0.393448 8_2:-65776212 Msa1262860:CDS
TTGTGATTGTAATTGCTTCA+TGG 0.397741 8_2:-65777315 Msa1262860:CDS
CCAATGGGGAACATGACAAC+TGG 0.405161 8_2:-65776861 Msa1262860:CDS
TGTTGTTTGAGGATGGCTTC+CGG 0.409237 8_2:-65777394 Msa1262860:exon
TTCCCAATATACTTTCCAAT+GGG 0.420160 8_2:-65776876 Msa1262860:CDS
CAATTATTGTGATTGTTTCA+AGG 0.431523 8_2:+65776256 Msa1262850:intron
AATTATTGTGATTGTTTCAA+GGG 0.436827 8_2:+65776257 Msa1262850:intron
AACTTGATCATTGTTGTTTG+AGG 0.443724 8_2:-65777405 Msa1262860:five_prime_UTR
TTAAATTAGGAGCATTCCAT+TGG 0.456921 8_2:+65776763 None:intergenic
GGCACTCACTCTGTTAGCCA+TGG 0.473924 8_2:-65776709 Msa1262860:CDS
TGAGAATTCATATATGGCAT+TGG 0.484778 8_2:+65776134 Msa1262850:intron
TGCACGACAGTTATCCATGT+TGG 0.489286 8_2:-65776213 Msa1262860:CDS
CCATGTCTTCACTGACAACA+TGG 0.491482 8_2:-65776731 Msa1262860:CDS
TGTGATTGTAATTGCTTCAT+GGG 0.493799 8_2:-65777314 Msa1262860:CDS
GCACTCACTCTGTTAGCCAT+GGG 0.500737 8_2:-65776708 Msa1262860:CDS
ATCATGGCATTCAAAGGTCT+AGG 0.516571 8_2:-65777285 Msa1262860:CDS
GCTTCATGGGCAATGAATCA+TGG 0.517487 8_2:-65777301 Msa1262860:CDS
TGAAATACAACACTAGATTG+AGG 0.520580 8_2:+65777338 None:intergenic
GGATCAAAATCAGTAGCCTT+AGG 0.522274 8_2:-65777370 Msa1262860:CDS
GCAAAGAGATTGAACGTGGT+TGG 0.522540 8_2:-65776548 Msa1262860:CDS
ATTCCCAATATACTTTCCAA+TGG 0.522562 8_2:-65776877 Msa1262860:CDS
CCAGTTGTCATGTTCCCCAT+TGG 0.525769 8_2:+65776861 None:intergenic
GAAATACAACACTAGATTGA+GGG 0.526099 8_2:+65777339 None:intergenic
ATTCAAAGGTCTAGGGAAGC+AGG 0.526713 8_2:-65777277 Msa1262860:intron
TAGTTTCATCAAATTTCAGT+TGG 0.532431 8_2:-65776159 Msa1262860:three_prime_UTR
TATATTGGGAATATGTGAGC+TGG 0.546271 8_2:+65776888 None:intergenic
TGATCATTGTTGTTTGAGGA+TGG 0.551046 8_2:-65777401 Msa1262860:exon
CAATGAATCATGGCATTCAA+AGG 0.553913 8_2:-65777291 Msa1262860:CDS
ATTGAGGGTTAAAAGGCCTA+AGG 0.558428 8_2:+65777354 None:intergenic
CACGACAGTTATCCATGTTG+GGG 0.573087 8_2:-65776211 Msa1262860:CDS
CCATGTTGTCAGTGAAGACA+TGG 0.585540 8_2:+65776731 None:intergenic
TCATGGCATTCAAAGGTCTA+GGG 0.602141 8_2:-65777284 Msa1262860:CDS
CACTCACTCTGTTAGCCATG+GGG 0.605084 8_2:-65776707 Msa1262860:intron
AACTATCTTGTAATTCTCTG+TGG 0.607338 8_2:+65776177 Msa1262850:intron
TGGTTGGACTGATTCAAACC+TGG 0.614574 8_2:-65776532 Msa1262860:intron
GAACTTCTGAAGCCCCAACA+TGG 0.616123 8_2:+65776199 Msa1262850:intron
TGGGAATATGTGAGCTGGAG+TGG 0.616192 8_2:+65776893 None:intergenic
GATTGAATAACATATTCCAA+TGG 0.624471 8_2:-65776779 Msa1262860:CDS
CGTGCATAACAGTTGTGTTG+CGG 0.634337 8_2:+65776230 Msa1262850:intron
TGTTTGAGGATGGCTTCCGG+AGG 0.647389 8_2:-65777391 Msa1262860:exon
CATGTCTTCACTGACAACAT+GGG 0.663676 8_2:-65776730 Msa1262860:CDS
TCCCAATATACTTTCCAATG+GGG 0.680310 8_2:-65776875 Msa1262860:CDS
GCATGCAAAGAGATTGAACG+TGG 0.746939 8_2:-65776552 Msa1262860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTCTAAAAAAAAACT+TGG + chr8_2:65775931-65775950 Msa1262850:intron 10.0%
!!! AAATTATCAATATTTTGTTT+TGG + chr8_2:65776380-65776399 Msa1262850:intron 10.0%
!!! TTTAATTCTAAAAAAAAACT+TGG + chr8_2:65775931-65775950 Msa1262850:intron 10.0%
!!! AAATTATCAATATTTTGTTT+TGG + chr8_2:65776380-65776399 Msa1262850:intron 10.0%
!!! TAATTCAAATTGATTTATCA+TGG - chr8_2:65776101-65776120 Msa1262860:three_prime_UTR 15.0%
!!! ATTGTGTTTTTTTATTTTAC+TGG - chr8_2:65776994-65777013 Msa1262860:intron 15.0%
!!! AACATCATATTCATTATTTT+TGG - chr8_2:65777053-65777072 Msa1262860:intron 15.0%
!!! TTTTTGTGTGAAAAAAATAA+AGG + chr8_2:65777289-65777308 None:intergenic 15.0%
!!! TAATTCAAATTGATTTATCA+TGG - chr8_2:65776101-65776120 Msa1262860:three_prime_UTR 15.0%
!!! ATTGTGTTTTTTTATTTTAC+TGG - chr8_2:65776994-65777013 Msa1262860:intron 15.0%
!!! AACATCATATTCATTATTTT+TGG - chr8_2:65777053-65777072 Msa1262860:intron 15.0%
!!! TTTTTGTGTGAAAAAAATAA+AGG + chr8_2:65777289-65777308 None:intergenic 15.0%
!!! TCCAAACAAATTTTTTTACA+AGG - chr8_2:65776302-65776321 Msa1262860:intron 20.0%
!!! TCCTTGTAAAAAAATTTGTT+TGG + chr8_2:65776306-65776325 Msa1262850:intron 20.0%
!!! TATAATTTGTTGACTTGAAT+TGG - chr8_2:65776413-65776432 Msa1262860:intron 20.0%
!! CTATATGTAATCAAAATAGA+GGG + chr8_2:65776690-65776709 None:intergenic 20.0%
!! ACTATATGTAATCAAAATAG+AGG + chr8_2:65776691-65776710 None:intergenic 20.0%
!! TATTACAATGTATTTGGATT+GGG + chr8_2:65776756-65776775 None:intergenic 20.0%
!! ATATTACAATGTATTTGGAT+TGG + chr8_2:65776757-65776776 None:intergenic 20.0%
!! AATCCAAATACATTGTAATA+TGG - chr8_2:65776756-65776775 Msa1262860:CDS 20.0%
!! ATCCAAATACATTGTAATAT+GGG - chr8_2:65776757-65776776 Msa1262860:CDS 20.0%
!! AATTATTGTGATTGTTTCAA+GGG + chr8_2:65777101-65777120 None:intergenic 20.0%
!! CATAAAAAAACAATATGATG+AGG + chr8_2:65777414-65777433 None:intergenic 20.0%
!!! TCCAAACAAATTTTTTTACA+AGG - chr8_2:65776302-65776321 Msa1262860:intron 20.0%
!!! TCCTTGTAAAAAAATTTGTT+TGG + chr8_2:65776306-65776325 Msa1262850:intron 20.0%
!!! TATAATTTGTTGACTTGAAT+TGG - chr8_2:65776413-65776432 Msa1262860:intron 20.0%
!! CTATATGTAATCAAAATAGA+GGG + chr8_2:65776690-65776709 None:intergenic 20.0%
!! ACTATATGTAATCAAAATAG+AGG + chr8_2:65776691-65776710 None:intergenic 20.0%
!! TATTACAATGTATTTGGATT+GGG + chr8_2:65776756-65776775 None:intergenic 20.0%
!! ATATTACAATGTATTTGGAT+TGG + chr8_2:65776757-65776776 None:intergenic 20.0%
!! AATCCAAATACATTGTAATA+TGG - chr8_2:65776756-65776775 Msa1262860:CDS 20.0%
!! ATCCAAATACATTGTAATAT+GGG - chr8_2:65776757-65776776 Msa1262860:CDS 20.0%
!! AATTATTGTGATTGTTTCAA+GGG + chr8_2:65777101-65777120 None:intergenic 20.0%
!! CATAAAAAAACAATATGATG+AGG + chr8_2:65777414-65777433 None:intergenic 20.0%
! CTAAAAAAAAACTTGGATTG+TGG + chr8_2:65775924-65775943 Msa1262850:intron 25.0%
!!! ACGTCAATTTTTTATTTGCA+TGG + chr8_2:65776171-65776190 Msa1262850:intron 25.0%
! GATTGAATAACATATTCCAA+TGG - chr8_2:65776576-65776595 Msa1262860:intron 25.0%
! AAAATAGAGGGATTAGAATA+AGG + chr8_2:65776678-65776697 None:intergenic 25.0%
!!! ACTCTATTTCTAGTGTAATT+TGG - chr8_2:65776919-65776938 Msa1262860:intron 25.0%
!! TAATTTGGTAGCTAAGAAAA+TGG - chr8_2:65776934-65776953 Msa1262860:intron 25.0%
! CAATTATTGTGATTGTTTCA+AGG + chr8_2:65777102-65777121 None:intergenic 25.0%
! TAGTTTCATCAAATTTCAGT+TGG - chr8_2:65777196-65777215 Msa1262860:intron 25.0%
!! TTCTTCTGAGAATTCATATA+TGG + chr8_2:65777230-65777249 None:intergenic 25.0%
! CTAAAAAAAAACTTGGATTG+TGG + chr8_2:65775924-65775943 Msa1262850:intron 25.0%
!!! ACGTCAATTTTTTATTTGCA+TGG + chr8_2:65776171-65776190 Msa1262850:intron 25.0%
! GATTGAATAACATATTCCAA+TGG - chr8_2:65776576-65776595 Msa1262860:intron 25.0%
! AAAATAGAGGGATTAGAATA+AGG + chr8_2:65776678-65776697 None:intergenic 25.0%
!!! ACTCTATTTCTAGTGTAATT+TGG - chr8_2:65776919-65776938 Msa1262860:intron 25.0%
!! TAATTTGGTAGCTAAGAAAA+TGG - chr8_2:65776934-65776953 Msa1262860:intron 25.0%
! CAATTATTGTGATTGTTTCA+AGG + chr8_2:65777102-65777121 None:intergenic 25.0%
! TAGTTTCATCAAATTTCAGT+TGG - chr8_2:65777196-65777215 Msa1262860:intron 25.0%
!! TTCTTCTGAGAATTCATATA+TGG + chr8_2:65777230-65777249 None:intergenic 25.0%
!! AACTTGATCATTGTTGTTTG+AGG - chr8_2:65775950-65775969 Msa1262860:three_prime_UTR 30.0%
GAAATACAACACTAGATTGA+GGG + chr8_2:65776019-65776038 Msa1262850:intron 30.0%
TGAAATACAACACTAGATTG+AGG + chr8_2:65776020-65776039 Msa1262850:intron 30.0%
TTGTGATTGTAATTGCTTCA+TGG - chr8_2:65776040-65776059 Msa1262860:three_prime_UTR 30.0%
TGTGATTGTAATTGCTTCAT+GGG - chr8_2:65776041-65776060 Msa1262860:three_prime_UTR 30.0%
! CTCAAGCAAAATAATTTGCA+GGG + chr8_2:65776142-65776161 Msa1262850:intron 30.0%
! ACTCAAGCAAAATAATTTGC+AGG + chr8_2:65776143-65776162 Msa1262850:intron 30.0%
GTCATATGTCGTGATTATAA+TGG - chr8_2:65776223-65776242 Msa1262860:CDS 30.0%
ATTCCCAATATACTTTCCAA+TGG - chr8_2:65776478-65776497 Msa1262860:intron 30.0%
TTCCCAATATACTTTCCAAT+GGG - chr8_2:65776479-65776498 Msa1262860:intron 30.0%
ATAATCTTCACTCTAATTGC+TGG - chr8_2:65776524-65776543 Msa1262860:intron 30.0%
!! TTAAATTAGGAGCATTCCAT+TGG + chr8_2:65776595-65776614 None:intergenic 30.0%
CACCCATATTACAATGTATT+TGG + chr8_2:65776762-65776781 None:intergenic 30.0%
ATGCAAATCTGCAAGAAAAT+TGG + chr8_2:65776787-65776806 None:intergenic 30.0%
GATATATAAAGAAGCTTACC+AGG + chr8_2:65776844-65776863 None:intergenic 30.0%
AACTATCTTGTAATTCTCTG+TGG + chr8_2:65777181-65777200 None:intergenic 30.0%
TGAGAATTCATATATGGCAT+TGG + chr8_2:65777224-65777243 None:intergenic 30.0%
!! AACTTGATCATTGTTGTTTG+AGG - chr8_2:65775950-65775969 Msa1262860:three_prime_UTR 30.0%
GAAATACAACACTAGATTGA+GGG + chr8_2:65776019-65776038 Msa1262850:intron 30.0%
TGAAATACAACACTAGATTG+AGG + chr8_2:65776020-65776039 Msa1262850:intron 30.0%
TTGTGATTGTAATTGCTTCA+TGG - chr8_2:65776040-65776059 Msa1262860:three_prime_UTR 30.0%
TGTGATTGTAATTGCTTCAT+GGG - chr8_2:65776041-65776060 Msa1262860:three_prime_UTR 30.0%
! CTCAAGCAAAATAATTTGCA+GGG + chr8_2:65776142-65776161 Msa1262850:intron 30.0%
! ACTCAAGCAAAATAATTTGC+AGG + chr8_2:65776143-65776162 Msa1262850:intron 30.0%
GTCATATGTCGTGATTATAA+TGG - chr8_2:65776223-65776242 Msa1262860:CDS 30.0%
ATTCCCAATATACTTTCCAA+TGG - chr8_2:65776478-65776497 Msa1262860:intron 30.0%
TTCCCAATATACTTTCCAAT+GGG - chr8_2:65776479-65776498 Msa1262860:intron 30.0%
ATAATCTTCACTCTAATTGC+TGG - chr8_2:65776524-65776543 Msa1262860:intron 30.0%
!! TTAAATTAGGAGCATTCCAT+TGG + chr8_2:65776595-65776614 None:intergenic 30.0%
CACCCATATTACAATGTATT+TGG + chr8_2:65776762-65776781 None:intergenic 30.0%
ATGCAAATCTGCAAGAAAAT+TGG + chr8_2:65776787-65776806 None:intergenic 30.0%
GATATATAAAGAAGCTTACC+AGG + chr8_2:65776844-65776863 None:intergenic 30.0%
AACTATCTTGTAATTCTCTG+TGG + chr8_2:65777181-65777200 None:intergenic 30.0%
TGAGAATTCATATATGGCAT+TGG + chr8_2:65777224-65777243 None:intergenic 30.0%
!! TGATCATTGTTGTTTGAGGA+TGG - chr8_2:65775954-65775973 Msa1262860:three_prime_UTR 35.0%
ACACTAGATTGAGGGTTAAA+AGG + chr8_2:65776011-65776030 Msa1262850:intron 35.0%
CAATGAATCATGGCATTCAA+AGG - chr8_2:65776064-65776083 Msa1262860:three_prime_UTR 35.0%
CATCAATTTATTGAGCCACT+TGG - chr8_2:65776199-65776218 Msa1262860:CDS 35.0%
TATATTGGGAATATGTGAGC+TGG + chr8_2:65776470-65776489 Msa1262850:intron 35.0%
TCCCAATATACTTTCCAATG+GGG - chr8_2:65776480-65776499 Msa1262860:intron 35.0%
! TCCCCATTGGAAAGTATATT+GGG + chr8_2:65776484-65776503 Msa1262850:intron 35.0%
! TTCCCCATTGGAAAGTATAT+TGG + chr8_2:65776485-65776504 Msa1262850:intron 35.0%
ATGGCTGCAGCATTTAAATT+AGG + chr8_2:65776608-65776627 None:intergenic 35.0%
!! TGATCATTGTTGTTTGAGGA+TGG - chr8_2:65775954-65775973 Msa1262860:three_prime_UTR 35.0%
ACACTAGATTGAGGGTTAAA+AGG + chr8_2:65776011-65776030 Msa1262850:intron 35.0%
CAATGAATCATGGCATTCAA+AGG - chr8_2:65776064-65776083 Msa1262860:three_prime_UTR 35.0%
CATCAATTTATTGAGCCACT+TGG - chr8_2:65776199-65776218 Msa1262860:CDS 35.0%
TATATTGGGAATATGTGAGC+TGG + chr8_2:65776470-65776489 Msa1262850:intron 35.0%
TCCCAATATACTTTCCAATG+GGG - chr8_2:65776480-65776499 Msa1262860:intron 35.0%
! TCCCCATTGGAAAGTATATT+GGG + chr8_2:65776484-65776503 Msa1262850:intron 35.0%
! TTCCCCATTGGAAAGTATAT+TGG + chr8_2:65776485-65776504 Msa1262850:intron 35.0%
ATGGCTGCAGCATTTAAATT+AGG + chr8_2:65776608-65776627 None:intergenic 35.0%
GGATCAAAATCAGTAGCCTT+AGG - chr8_2:65775985-65776004 Msa1262860:three_prime_UTR 40.0%
ATTGAGGGTTAAAAGGCCTA+AGG + chr8_2:65776004-65776023 Msa1262850:intron 40.0%
ATCATGGCATTCAAAGGTCT+AGG - chr8_2:65776070-65776089 Msa1262860:three_prime_UTR 40.0%
TCATGGCATTCAAAGGTCTA+GGG - chr8_2:65776071-65776090 Msa1262860:three_prime_UTR 40.0%
!! GTGATTATAATGGACCACGT+AGG - chr8_2:65776233-65776252 Msa1262860:CDS 40.0%
!! ACTCTAATTGCTGGTGTTGT+GGG - chr8_2:65776533-65776552 Msa1262860:CDS 40.0%
GGTTTCACTACATCAGTAAC+TGG - chr8_2:65776554-65776573 Msa1262860:CDS 40.0%
CATGTCTTCACTGACAACAT+GGG - chr8_2:65776625-65776644 Msa1262860:intron 40.0%
GGATCAAAATCAGTAGCCTT+AGG - chr8_2:65775985-65776004 Msa1262860:three_prime_UTR 40.0%
ATTGAGGGTTAAAAGGCCTA+AGG + chr8_2:65776004-65776023 Msa1262850:intron 40.0%
ATCATGGCATTCAAAGGTCT+AGG - chr8_2:65776070-65776089 Msa1262860:three_prime_UTR 40.0%
TCATGGCATTCAAAGGTCTA+GGG - chr8_2:65776071-65776090 Msa1262860:three_prime_UTR 40.0%
!! GTGATTATAATGGACCACGT+AGG - chr8_2:65776233-65776252 Msa1262860:CDS 40.0%
!! ACTCTAATTGCTGGTGTTGT+GGG - chr8_2:65776533-65776552 Msa1262860:CDS 40.0%
GGTTTCACTACATCAGTAAC+TGG - chr8_2:65776554-65776573 Msa1262860:CDS 40.0%
CATGTCTTCACTGACAACAT+GGG - chr8_2:65776625-65776644 Msa1262860:intron 40.0%
! TGTTGTTTGAGGATGGCTTC+CGG - chr8_2:65775961-65775980 Msa1262860:three_prime_UTR 45.0%
!!! GGCTACTGATTTTGATCCTC+CGG + chr8_2:65775983-65776002 Msa1262850:intron 45.0%
GCTTCATGGGCAATGAATCA+TGG - chr8_2:65776054-65776073 Msa1262860:three_prime_UTR 45.0%
ATTCAAAGGTCTAGGGAAGC+AGG - chr8_2:65776078-65776097 Msa1262860:three_prime_UTR 45.0%
TCACGACATATGACACCAAG+TGG + chr8_2:65776217-65776236 Msa1262850:intron 45.0%
TTAGTCACTAACGTCCTACG+TGG + chr8_2:65776250-65776269 Msa1262850:intron 45.0%
! CACTCTAATTGCTGGTGTTG+TGG - chr8_2:65776532-65776551 Msa1262860:intron 45.0%
CCATGTTGTCAGTGAAGACA+TGG + chr8_2:65776627-65776646 None:intergenic 45.0%
CCATGTCTTCACTGACAACA+TGG - chr8_2:65776624-65776643 Msa1262860:intron 45.0%
TTAGAATAAGGCTGACCCCA+TGG + chr8_2:65776666-65776685 None:intergenic 45.0%
GCATGCAAAGAGATTGAACG+TGG - chr8_2:65776803-65776822 Msa1262860:CDS 45.0%
GCAAAGAGATTGAACGTGGT+TGG - chr8_2:65776807-65776826 Msa1262860:CDS 45.0%
! TGGTTGGACTGATTCAAACC+TGG - chr8_2:65776823-65776842 Msa1262860:CDS 45.0%
CGTGCATAACAGTTGTGTTG+CGG + chr8_2:65777128-65777147 None:intergenic 45.0%
TGCACGACAGTTATCCATGT+TGG - chr8_2:65777142-65777161 Msa1262860:intron 45.0%
GCACGACAGTTATCCATGTT+GGG - chr8_2:65777143-65777162 Msa1262860:intron 45.0%
CACGACAGTTATCCATGTTG+GGG - chr8_2:65777144-65777163 Msa1262860:intron 45.0%
! TGTTGTTTGAGGATGGCTTC+CGG - chr8_2:65775961-65775980 Msa1262860:three_prime_UTR 45.0%
!!! GGCTACTGATTTTGATCCTC+CGG + chr8_2:65775983-65776002 Msa1262850:intron 45.0%
GCTTCATGGGCAATGAATCA+TGG - chr8_2:65776054-65776073 Msa1262860:three_prime_UTR 45.0%
ATTCAAAGGTCTAGGGAAGC+AGG - chr8_2:65776078-65776097 Msa1262860:three_prime_UTR 45.0%
TCACGACATATGACACCAAG+TGG + chr8_2:65776217-65776236 Msa1262850:intron 45.0%
TTAGTCACTAACGTCCTACG+TGG + chr8_2:65776250-65776269 Msa1262850:intron 45.0%
! CACTCTAATTGCTGGTGTTG+TGG - chr8_2:65776532-65776551 Msa1262860:intron 45.0%
CCATGTTGTCAGTGAAGACA+TGG + chr8_2:65776627-65776646 None:intergenic 45.0%
CCATGTCTTCACTGACAACA+TGG - chr8_2:65776624-65776643 Msa1262860:intron 45.0%
TTAGAATAAGGCTGACCCCA+TGG + chr8_2:65776666-65776685 None:intergenic 45.0%
GCATGCAAAGAGATTGAACG+TGG - chr8_2:65776803-65776822 Msa1262860:CDS 45.0%
GCAAAGAGATTGAACGTGGT+TGG - chr8_2:65776807-65776826 Msa1262860:CDS 45.0%
! TGGTTGGACTGATTCAAACC+TGG - chr8_2:65776823-65776842 Msa1262860:CDS 45.0%
CGTGCATAACAGTTGTGTTG+CGG + chr8_2:65777128-65777147 None:intergenic 45.0%
TGCACGACAGTTATCCATGT+TGG - chr8_2:65777142-65777161 Msa1262860:intron 45.0%
GCACGACAGTTATCCATGTT+GGG - chr8_2:65777143-65777162 Msa1262860:intron 45.0%
CACGACAGTTATCCATGTTG+GGG - chr8_2:65777144-65777163 Msa1262860:intron 45.0%
TGGGAATATGTGAGCTGGAG+TGG + chr8_2:65776465-65776484 Msa1262850:intron 50.0%
CCAGTTGTCATGTTCCCCAT+TGG + chr8_2:65776497-65776516 Msa1262850:intron 50.0%
CCAATGGGGAACATGACAAC+TGG - chr8_2:65776494-65776513 Msa1262860:intron 50.0%
GCACTCACTCTGTTAGCCAT+GGG - chr8_2:65776647-65776666 Msa1262860:intron 50.0%
CACTCACTCTGTTAGCCATG+GGG - chr8_2:65776648-65776667 Msa1262860:intron 50.0%
! TGATTGTTTCAAGGGTGCGC+TGG + chr8_2:65777093-65777112 None:intergenic 50.0%
!! GAACTTCTGAAGCCCCAACA+TGG + chr8_2:65777159-65777178 None:intergenic 50.0%
TGGGAATATGTGAGCTGGAG+TGG + chr8_2:65776465-65776484 Msa1262850:intron 50.0%
CCAGTTGTCATGTTCCCCAT+TGG + chr8_2:65776497-65776516 Msa1262850:intron 50.0%
CCAATGGGGAACATGACAAC+TGG - chr8_2:65776494-65776513 Msa1262860:intron 50.0%
GCACTCACTCTGTTAGCCAT+GGG - chr8_2:65776647-65776666 Msa1262860:intron 50.0%
CACTCACTCTGTTAGCCATG+GGG - chr8_2:65776648-65776667 Msa1262860:intron 50.0%
! TGATTGTTTCAAGGGTGCGC+TGG + chr8_2:65777093-65777112 None:intergenic 50.0%
!! GAACTTCTGAAGCCCCAACA+TGG + chr8_2:65777159-65777178 None:intergenic 50.0%
TGTTTGAGGATGGCTTCCGG+AGG - chr8_2:65775964-65775983 Msa1262860:three_prime_UTR 55.0%
GGCACTCACTCTGTTAGCCA+TGG - chr8_2:65776646-65776665 Msa1262860:intron 55.0%
TGTTTGAGGATGGCTTCCGG+AGG - chr8_2:65775964-65775983 Msa1262860:three_prime_UTR 55.0%
GGCACTCACTCTGTTAGCCA+TGG - chr8_2:65776646-65776665 Msa1262860:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 65775918 65777459 65775918 ID=Msa1262860;Name=Msa1262860
chr8_2 mRNA 65775918 65777459 65775918 ID=Msa1262860-mRNA-1;Parent=Msa1262860;Name=Msa1262860-mRNA-1;_AED=0.07;_eAED=0.07;_QI=55|1|1|1|1|1|4|261|165
chr8_2 exon 65775918 65776283 65775918 ID=Msa1262860-mRNA-1:exon:21409;Parent=Msa1262860-mRNA-1
chr8_2 exon 65776533 65776578 65776533 ID=Msa1262860-mRNA-1:exon:21408;Parent=Msa1262860-mRNA-1
chr8_2 exon 65776708 65776927 65776708 ID=Msa1262860-mRNA-1:exon:21407;Parent=Msa1262860-mRNA-1
chr8_2 exon 65777278 65777459 65777278 ID=Msa1262860-mRNA-1:exon:21406;Parent=Msa1262860-mRNA-1
chr8_2 five_prime_UTR 65777405 65777459 65777405 ID=Msa1262860-mRNA-1:five_prime_utr;Parent=Msa1262860-mRNA-1
chr8_2 CDS 65777278 65777404 65777278 ID=Msa1262860-mRNA-1:cds;Parent=Msa1262860-mRNA-1
chr8_2 CDS 65776708 65776927 65776708 ID=Msa1262860-mRNA-1:cds;Parent=Msa1262860-mRNA-1
chr8_2 CDS 65776533 65776578 65776533 ID=Msa1262860-mRNA-1:cds;Parent=Msa1262860-mRNA-1
chr8_2 CDS 65776179 65776283 65776179 ID=Msa1262860-mRNA-1:cds;Parent=Msa1262860-mRNA-1
chr8_2 three_prime_UTR 65775918 65776178 65775918 ID=Msa1262860-mRNA-1:three_prime_utr;Parent=Msa1262860-mRNA-1
Gene Sequence

>Msa1262860

ATGGCTTCCGGAGGATCAAAATCAGTAGCCTTAGGCCTTTTAACCCTCAATCTAGTGTTGTATTTCATTGTGATTGTAATTGCTTCATGGGCAATGAATCATGGCATTCAAAGGTCTAGGGAAGCAGCATCAGTTTTGACCACTCCAGCTCACATATTCCCAATATACTTTCCAATGGGGAACATGACAACTGGTTTTTTCATAATCTTCACTCTAATTGCTGGTGTTGTGGGTTTCACTACATCAGTAACTGGATTGAATAACATATTCCAATGGAATGCTCCTAATTTAAATGCTGCAGCCATGTCTTCACTGACAACATGGGCACTCACTCTGTTAGCCATGGGATTTGCATGCAAAGAGATTGAACGTGGTTGGACTGATTCAAACCTGCGCACCCTTGAAACAATCACAATAATTGTAACCGCAACACAACTGTTATGCACGACAGTTATCCATGTTGGGGCTTCAGAAGTTCCACAGAGAATTACAAGATAG
Protein sequence

>Msa1262860

MASGGSKSVALGLLTLNLVLYFIVIVIASWAMNHGIQRSREAASVLTTPAHIFPIYFPMGNMTTGFFIIFTLIAGVVGFTTSVTGLNNIFQWNAPNLNAAAMSSLTTWALTLLAMGFACKEIERGWTDSNLRTLETITIIVTATQLLCTTVIHVGASEVPQRITR*