AlfalfaGEDB | Msa1301170

M. sativa cultivar zhongmu-4 / Msa1301170

KEGG: K07297
GO: GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009743 GO:0009744 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0010033 GO:0010646 GO:0010648 GO:0023051 GO:0023057 GO:0034285 GO:0042221 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:1901419 GO:1901420 GO:1901700 GO:1905957 GO:1905958
PFAM: PF03006.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301170	sp\|Q93ZH9\|HHP1_ARATH	55.000	360	115	3	4	363	20	332	9.41e-138	398

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301170	G7JFE9	99.180	366	2	1	1	366	1	365	0.0	753

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0035090	Msa1301170	0.804123	2.512725e-49	-8.615850e-47
Msa0094060	Msa1301170	0.801710	7.919152e-49	-8.615850e-47
Msa0096230	Msa1301170	0.820812	5.628634e-53	-8.615850e-47
Msa0130120	Msa1301170	0.805443	1.331903e-49	-8.615850e-47
Msa0175900	Msa1301170	0.809409	1.919800e-50	-8.615850e-47
Msa0207530	Msa1301170	0.808959	2.396690e-50	-8.615850e-47
Msa0207900	Msa1301170	0.804322	2.284118e-49	-8.615850e-47
Msa0392410	Msa1301170	0.840019	1.118854e-57	-8.615850e-47
Msa0392450	Msa1301170	0.829308	5.536729e-55	-8.615850e-47
Msa0392480	Msa1301170	0.833387	5.491273e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301170	MtrunA17_Chr4g0064481	99.180	366	2	1	1	366	1	365	0.0	753
Msa1301170	MtrunA17_Chr2g0305481	49.258	337	143	5	39	358	4	329	8.01e-107	317
Msa1301170	MtrunA17_Chr5g0401021	45.480	354	159	7	42	363	28	379	1.53e-96	292
Msa1301170	MtrunA17_Chr3g0134921	41.371	394	184	6	17	363	3	396	1.71e-96	292

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301170	AT5G20270.1	55.000	360	115	3	4	363	20	332	9.57e-139	398
Msa1301170	AT4G37680.3	43.401	394	179	9	10	363	1	390	1.72e-98	298
Msa1301170	AT4G37680.4	43.401	394	179	9	10	363	1	390	1.72e-98	298
Msa1301170	AT2G24150.1	42.614	352	179	5	10	356	1	334	8.43e-98	294
Msa1301170	AT4G37680.1	43.750	384	174	8	18	363	6	385	1.60e-97	295
Msa1301170	AT4G37680.5	43.750	384	174	8	18	363	6	385	1.60e-97	295
Msa1301170	AT4G37680.2	43.750	384	174	8	18	363	6	385	1.60e-97	295
Msa1301170	AT4G30850.2	44.543	339	163	4	37	358	20	350	1.37e-96	291
Msa1301170	AT4G30850.1	44.543	339	163	4	37	358	20	350	1.37e-96	291
Msa1301170	AT4G38320.1	43.164	373	170	8	18	352	6	374	2.21e-91	279

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Find 104 sgRNAs with CRISPR-Local

Find 467 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACAAACAACCTTTGATT+TGG	0.307947	8_3:-63116222	Msa1301170:five_prime_UTR
AAGGTTTGGCACTATGTATG+TGG	0.496101	8_3:+63116411	None:intergenic
AATCTATTCTTAACAGAAAC+AGG	0.333630	8_3:+63107455	Msa1301180:intron
ACAACATAGCTCGAAATGCG+CGG	0.643746	8_3:+63107269	Msa1301180:CDS
ACACTCTCCTCCGTAAGGTT+TGG	0.472006	8_3:+63116397	None:intergenic
ACAGAAACAGGTTTAAGTCA+TGG	0.515069	8_3:+63107467	Msa1301180:intron
ACTAGCATATGAATGTGTCA+TGG	0.642425	8_3:-63107164	Msa1301170:CDS
AGACAAATTGTGTTGTCAAG+AGG	0.634394	8_3:-63116150	Msa1301170:CDS
AGACCCAAACTAATTTATGA+TGG	0.535015	8_3:+63116453	None:intergenic
AGCTATGTTGTTCTGTTCAA+TGG	0.349396	8_3:-63107257	Msa1301170:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACCCAAACTAATTTATGA+TGG	+	chr8_3:63106952-63106971	None:intergenic	30.0%
AACACAAACAACCTTTGATT+TGG	-	chr8_3:63107180-63107199	Msa1301170:CDS	30.0%
CGACATCTTTATATTATCCT+TGG	+	chr8_3:63107324-63107343	Msa1301180:intron	30.0%
ATATAAAGATGTCGCATGAT+CGG	-	chr8_3:63107330-63107349	Msa1301170:CDS	30.0%
ATCCAACTAAATGTCTGAAA+AGG	+	chr8_3:63107638-63107657	Msa1301180:intron	30.0%
TTTATGTGTCTATATGTGAG+AGG	-	chr8_3:63107768-63107787	Msa1301170:intron	30.0%
AAAGAATAAGAGAACCAATC+TGG	-	chr8_3:63107817-63107836	Msa1301170:CDS	30.0%
AATAGTAACATATCCCTCAA+TGG	-	chr8_3:63108617-63108636	Msa1301170:intron	30.0%
TGTGCAGAATATTAATTGGA+GGG	+	chr8_3:63108763-63108782	Msa1301180:intron	30.0%
GTAGTGTGCAGAATATTAAT+TGG	+	chr8_3:63108767-63108786	Msa1301180:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	63106946	63116478	63106946	ID=Msa1301170;Name=Msa1301170
chr8_3	mRNA	63106946	63116478	63106946	ID=Msa1301170-mRNA-1;Parent=Msa1301170;Name=Msa1301170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=293\|1\|1\|1\|1\|1\|4\|24\|366
chr8_3	exon	63106946	63107650	63106946	ID=Msa1301170-mRNA-1:exon:18830;Parent=Msa1301170-mRNA-1
chr8_3	exon	63107813	63107864	63107813	ID=Msa1301170-mRNA-1:exon:18829;Parent=Msa1301170-mRNA-1
chr8_3	exon	63115691	63115780	63115691	ID=Msa1301170-mRNA-1:exon:18828;Parent=Msa1301170-mRNA-1
chr8_3	exon	63115908	63116478	63115908	ID=Msa1301170-mRNA-1:exon:18827;Parent=Msa1301170-mRNA-1
chr8_3	five_prime_UTR	63116186	63116478	63116186	ID=Msa1301170-mRNA-1:five_prime_utr;Parent=Msa1301170-mRNA-1
chr8_3	CDS	63115908	63116185	63115908	ID=Msa1301170-mRNA-1:cds;Parent=Msa1301170-mRNA-1
chr8_3	CDS	63115691	63115780	63115691	ID=Msa1301170-mRNA-1:cds;Parent=Msa1301170-mRNA-1
chr8_3	CDS	63107813	63107864	63107813	ID=Msa1301170-mRNA-1:cds;Parent=Msa1301170-mRNA-1
chr8_3	CDS	63106970	63107650	63106970	ID=Msa1301170-mRNA-1:cds;Parent=Msa1301170-mRNA-1
chr8_3	three_prime_UTR	63106946	63106969	63106946	ID=Msa1301170-mRNA-1:three_prime_utr;Parent=Msa1301170-mRNA-1

Gene Sequence

>Msa1301170

ATGAACTATTGTGAGACAAATTGTGTTGTCAAGAGGAACAAAGGAGGTAAAGAAACTGATGAAACCTTGTCTTTTACACAGTCCAAGGATAATATAAAGATGTCGCATGATCGGAACAAAGGAAAACAACAACGTTACGCTCTTGTGTCTTTTATGGAGCTACCGAATTATATGAAGGATAATGAATATATTTTACGTTATTACAGAGCCAATTGGCCTTTGAAAGAAGCTTTCTTCAGTCTCTTTCGTTGGCATAATGAAACTTTGAATGTTTGGACACATTTAGTTGGATTTATTCTGTTTCTTGGGTTGACCTTGGCCAATTTGATGAAGCCTCATGTGGTGGATCTCTTACAACAATTTACAAGGTCTATCTCTTCAGGTGGTGAAAAAAATGTTTCTGATAGCATCAAAGTTCTGGGAACGGCGTTACTCTTTGATTTAAACCATCAATTACCATTGGCAATGGAAGTTGAATCCTTAGAATTTGTTATAGCAAGGTGGCCATTCTTTGTCTTCTTAGGTGGTTCAATGTTCTGTCTCCTCTCTAGCAGCATTTGCCACCTCTTTTCATGTCACTCCCATGACTTAAACCTGTTTCTGTTAAGAATAGATTATGTCGGAATCGCTGTCATGATCATAACCTCATTCTTCCCTCAGATTTACTATGTTTTCCTATGTCAACCTCATTGGCAACTCATCTACCTAGCCGGTATTACAGCCATGGGACTATTCACCATAGTAACATTGCTTTCCCCATCACTTTCTACCGGTAAACACCGCGCATTTCGAGCTATGTTGTTCTGTTCAATGGGACTTTTCGGCATTGTGCCTGCAGTTCATGCTTGTGTTGCAAATTGGGGAAATCCTCGACGCAATGTTACACTAGCATATGAATGTGTCATGGCATTGTCTTACTTGATAGGGACTTTGTTCTATGTGACGCGGATACCGGAAAGATGGAGGCCTGGTTGGTTTGATTTAGCAGGACATAGTCATCAAATATTTCATATTTTGGTTGTGGTTGGTGCATTATCACATTATGCAGCTACACTTAAAATGCTAGAATGGCGTGACACTTTTGGGTGTGACAAGGCTTAA

Protein sequence

>Msa1301170

MNYCETNCVVKRNKGGKETDETLSFTQSKDNIKMSHDRNKGKQQRYALVSFMELPNYMKDNEYILRYYRANWPLKEAFFSLFRWHNETLNVWTHLVGFILFLGLTLANLMKPHVVDLLQQFTRSISSGGEKNVSDSIKVLGTALLFDLNHQLPLAMEVESLEFVIARWPFFVFLGGSMFCLLSSSICHLFSCHSHDLNLFLLRIDYVGIAVMIITSFFPQIYYVFLCQPHWQLIYLAGITAMGLFTIVTLLSPSLSTGKHRAFRAMLFCSMGLFGIVPAVHACVANWGNPRRNVTLAYECVMALSYLIGTLFYVTRIPERWRPGWFDLAGHSHQIFHILVVVGALSHYAATLKMLEWRDTFGCDKA*