AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215460 MtrunA17_Chr4g0064481 99.180 366 2 1 1 366 1 365 0.0 753
Msa1215460 MtrunA17_Chr2g0305481 49.258 337 143 5 39 358 4 329 8.01e-107 317
Msa1215460 MtrunA17_Chr5g0401021 45.480 354 159 7 42 363 28 379 1.53e-96 292
Msa1215460 MtrunA17_Chr3g0134921 41.371 394 184 6 17 363 3 396 1.71e-96 292
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215460 AT5G20270.1 55.000 360 115 3 4 363 20 332 9.57e-139 398
Msa1215460 AT4G37680.3 43.401 394 179 9 10 363 1 390 1.72e-98 298
Msa1215460 AT4G37680.4 43.401 394 179 9 10 363 1 390 1.72e-98 298
Msa1215460 AT2G24150.1 42.614 352 179 5 10 356 1 334 8.43e-98 294
Msa1215460 AT4G37680.1 43.750 384 174 8 18 363 6 385 1.60e-97 295
Msa1215460 AT4G37680.5 43.750 384 174 8 18 363 6 385 1.60e-97 295
Msa1215460 AT4G37680.2 43.750 384 174 8 18 363 6 385 1.60e-97 295
Msa1215460 AT4G30850.2 44.543 339 163 4 37 358 20 350 1.37e-96 291
Msa1215460 AT4G30850.1 44.543 339 163 4 37 358 20 350 1.37e-96 291
Msa1215460 AT4G38320.1 43.164 373 170 8 18 352 6 374 2.21e-91 279
Msa1215460 AT2G24150.2 51.295 193 94 0 165 357 54 246 1.73e-69 219
Msa1215460 AT2G40710.1 53.763 93 43 0 271 363 1 93 3.89e-29 108
Msa1215460 AT4G38290.1 59.459 74 29 1 291 363 35 108 1.89e-25 99.4

Find 104 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCAAATATTTCATATTT+TGG 0.119405 8_1:-76712380 Msa1215460:CDS
TGCACCAACTGCAGCATTTA+AGG 0.156919 8_1:+76714865 None:intergenic
GTTGGATTTATTCTGTTTCT+TGG 0.171486 8_1:-76714246 Msa1215460:CDS
TTCATGCTTGTGTTGCAAAT+TGG 0.232497 8_1:-76712537 Msa1215460:CDS
TGGCCATTCTTTGTCTTCTT+AGG 0.247470 8_1:-76712874 Msa1215460:CDS
CAACCAGGCCTCCATCTTTC+CGG 0.258607 8_1:+76712424 None:intergenic
TCAGCCATCATAAATTAGTT+TGG 0.266724 8_1:-76714951 Msa1215460:five_prime_UTR
GTACATAGGTCTATCTCTTC+AGG 0.282931 8_1:-76713177 Msa1215460:intron
CCTTGCTATAACAAATTCTA+AGG 0.299308 8_1:+76712897 None:intergenic
GTGTGACAAGGCTTAAATTA+AGG 0.306890 8_1:-76712290 Msa1215460:exon
AACACAAACAACCTTTGATT+TGG 0.307947 8_1:-76714715 Msa1215460:five_prime_UTR
ATGGCATTGTCTTACTTGAT+AGG 0.313226 8_1:-76712472 Msa1215460:CDS
CACAATAGTTCATCTCTGTT+TGG 0.326565 8_1:+76714666 None:intergenic
CACATGAGGCTTCATCAAAT+TGG 0.331471 8_1:+76714212 None:intergenic
TTGGATTTATTCTGTTTCTT+GGG 0.332306 8_1:-76714245 Msa1215460:CDS
AATCTATTCTTAACAGAAAC+AGG 0.333630 8_1:+76712782 None:intergenic
CTAGAATGGCGTGACACTTT+TGG 0.338813 8_1:-76712313 Msa1215460:CDS
AGCTATGTTGTTCTGTTCAA+TGG 0.349396 8_1:-76712584 Msa1215460:CDS
TTCTGATAGCATCAAAGTTC+TGG 0.354226 8_1:-76713139 Msa1215460:intron
TCGAAATGCGCGGTGTTTAC+CGG 0.364215 8_1:+76712606 None:intergenic
CGACATCTTTATATTATCCT+TGG 0.366302 8_1:+76714574 None:intergenic
CAGCCATCATAAATTAGTTT+GGG 0.367155 8_1:-76714950 Msa1215460:five_prime_UTR
TCATGCTTGTGTTGCAAATT+GGG 0.369516 8_1:-76712536 Msa1215460:CDS
TCATTATCCTTCATATAATT+CGG 0.373033 8_1:+76714494 None:intergenic
CCATTGCCAATGGTAATTGA+TGG 0.375582 8_1:+76712929 None:intergenic
ATAATGAAACTTTGAATGTT+TGG 0.388241 8_1:-76714403 Msa1215460:CDS
AGCTTCTTTCAAAGGCCAAT+TGG 0.392420 8_1:+76714448 None:intergenic
GCACCAACTGCAGCATTTAA+GGG 0.425405 8_1:+76714866 None:intergenic
GTTCTATGTGACGCGGATAC+CGG 0.430826 8_1:-76712443 Msa1215460:CDS
CTTTCTTCAGTCTCTTTCGT+TGG 0.437703 8_1:-76714427 Msa1215460:CDS
CCTGGTTGGTTTGATTTAGC+AGG 0.442111 8_1:-76712409 Msa1215460:CDS
TCTGTTTCTTGGGTTGACCT+TGG 0.451875 8_1:-76714235 Msa1215460:CDS
TACGTTATTACAGAGCCAAT+TGG 0.471460 8_1:-76714463 Msa1215460:CDS
ACACTCTCCTCCGTAAGGTT+TGG 0.472006 8_1:+76714891 None:intergenic
GCTATGTTGTTCTGTTCAAT+GGG 0.476457 8_1:-76712583 Msa1215460:CDS
CCATTCTTTGTCTTCTTAGG+TGG 0.483745 8_1:-76712871 Msa1215460:CDS
TAGTCCCATGGCTGTAATAC+CGG 0.489415 8_1:+76712666 None:intergenic
CTACACTTAAAATGCTAGAA+TGG 0.493476 8_1:-76712327 Msa1215460:CDS
CAGAAACAGGTTTAAGTCAT+GGG 0.494110 8_1:+76712795 None:intergenic
AAGGTTTGGCACTATGTATG+TGG 0.496101 8_1:+76714905 None:intergenic
CGGAAAGATGGAGGCCTGGT+TGG 0.498832 8_1:-76712423 Msa1215460:CDS
CTGAAGAAAGCTTCTTTCAA+AGG 0.500853 8_1:+76714440 None:intergenic
AGGTTTGGCACTATGTATGT+GGG 0.502161 8_1:+76714906 None:intergenic
ATCTCTTACAACAATTTACA+AGG 0.502630 8_1:-76714185 Msa1215460:intron
TGGCATTGTCTTACTTGATA+GGG 0.509976 8_1:-76712471 Msa1215460:CDS
ACAGAAACAGGTTTAAGTCA+TGG 0.515069 8_1:+76712794 None:intergenic
TTTAAACCATCAATTACCAT+TGG 0.515618 8_1:-76712935 Msa1215460:CDS
CTTTCCCCATCACTTTCTAC+CGG 0.516838 8_1:-76712625 Msa1215460:CDS
GGTGGCAAATGCTGCTAGAG+AGG 0.518257 8_1:+76712833 None:intergenic
CTGTTAAGAATAGATTATGT+CGG 0.524523 8_1:-76712775 Msa1215460:CDS
TGGGGAAAGCAATGTTACTA+TGG 0.530611 8_1:+76712639 None:intergenic
ATACATAGTGCCAAACCTTA+CGG 0.534222 8_1:-76714901 Msa1215460:five_prime_UTR
AGACCCAAACTAATTTATGA+TGG 0.535015 8_1:+76714947 None:intergenic
TGACGCGGATACCGGAAAGA+TGG 0.537670 8_1:-76712435 Msa1215460:CDS
CCTTAGAATTTGTTATAGCA+AGG 0.538068 8_1:-76712897 Msa1215460:CDS
GTTTACCGGTAGAAAGTGAT+GGG 0.539453 8_1:+76712620 None:intergenic
ATTTCTCTGACTTGGTTGTG+TGG 0.539970 8_1:+76714808 None:intergenic
CCATCAATTACCATTGGCAA+TGG 0.541784 8_1:-76712929 Msa1215460:CDS
CCACCTAAGAAGACAAAGAA+TGG 0.542364 8_1:+76712871 None:intergenic
GAGCATATATTTCTCTGACT+TGG 0.546616 8_1:+76714800 None:intergenic
ATATAAAGATGTCGCATGAT+CGG 0.549561 8_1:-76714565 Msa1215460:CDS
AGGAAAACATAGTAAATCTG+AGG 0.556599 8_1:+76712721 None:intergenic
ATCAAACAAAACCAAATCAA+AGG 0.558711 8_1:+76714704 None:intergenic
CATGCTTGTGTTGCAAATTG+GGG 0.564814 8_1:-76712535 Msa1215460:CDS
TGTTTACCGGTAGAAAGTGA+TGG 0.568335 8_1:+76712619 None:intergenic
ATACCGGAAAGATGGAGGCC+TGG 0.568351 8_1:-76712427 Msa1215460:CDS
GCAACACAAGCATGAACTGC+AGG 0.575233 8_1:+76712544 None:intergenic
CTTGGACTGTGTAAAAGACA+AGG 0.577522 8_1:+76714592 None:intergenic
GGGACACACTCTCCTCCGTA+AGG 0.578007 8_1:+76714886 None:intergenic
GATTCAACTTCCATTGCCAA+TGG 0.580416 8_1:+76712919 None:intergenic
TGTTGATGATGGAGTAATGA+GGG 0.582660 8_1:+76714836 None:intergenic
GTGTGGCTAAATGTTGATGA+TGG 0.583455 8_1:+76714825 None:intergenic
CATGGGAGTGACATGAAAAG+AGG 0.583926 8_1:+76712812 None:intergenic
GGAGCTACCGAATTATATGA+AGG 0.585057 8_1:-76714501 Msa1215460:CDS
TGGCAACTCATCTACCTAGC+CGG 0.586984 8_1:-76712685 Msa1215460:CDS
TGTGTTGTCAAGAGGAACAA+AGG 0.596024 8_1:-76714635 Msa1215460:CDS
GGTTTGGCACTATGTATGTG+GGG 0.597181 8_1:+76714907 None:intergenic
GTTGCCAATGAGGTTGACAT+AGG 0.600058 8_1:+76712701 None:intergenic
CCATGGCTGTAATACCGGCT+AGG 0.606682 8_1:+76712671 None:intergenic
GGAAAACATAGTAAATCTGA+GGG 0.611187 8_1:+76712722 None:intergenic
TGTAAGAGATCCACCACATG+AGG 0.615952 8_1:+76714198 None:intergenic
TAGAATTTGTTATAGCAAGG+TGG 0.617513 8_1:-76712894 Msa1215460:CDS
AGGTAGATGAGTTGCCAATG+AGG 0.623505 8_1:+76712691 None:intergenic
ATGTCGCATGATCGGAACAA+AGG 0.623538 8_1:-76714557 Msa1215460:CDS
CGCGGATACCGGAAAGATGG+AGG 0.624928 8_1:-76712432 Msa1215460:CDS
CCTGCTAAATCAAACCAACC+AGG 0.625235 8_1:+76712409 None:intergenic
TGTCCCTTAAATGCTGCAGT+TGG 0.633833 8_1:-76714869 Msa1215460:five_prime_UTR
AGACAAATTGTGTTGTCAAG+AGG 0.634394 8_1:-76714643 Msa1215460:CDS
TTTGATGAAGCCTCATGTGG+TGG 0.642283 8_1:-76714208 Msa1215460:CDS
ACTAGCATATGAATGTGTCA+TGG 0.642425 8_1:-76712491 Msa1215460:CDS
ACAACATAGCTCGAAATGCG+CGG 0.643746 8_1:+76712596 None:intergenic
CTAGCCGGTATTACAGCCAT+GGG 0.645941 8_1:-76712670 Msa1215460:CDS
GTAAATCTGAGGGAAGAATG+AGG 0.646981 8_1:+76712732 None:intergenic
GGGAGTGACATGAAAAGAGG+TGG 0.647977 8_1:+76712815 None:intergenic
AGGCTTCATCAAATTGGCCA+AGG 0.660901 8_1:+76714218 None:intergenic
TACTATGGTGAATAGTCCCA+TGG 0.663061 8_1:+76712654 None:intergenic
CATAGGTCTATCTCTTCAGG+TGG 0.665575 8_1:-76713174 Msa1215460:intron
CAATTTGATGAAGCCTCATG+TGG 0.668778 8_1:-76714211 Msa1215460:CDS
GGACTTTGTTCTATGTGACG+CGG 0.692634 8_1:-76712450 Msa1215460:CDS
ATGTTGATGATGGAGTAATG+AGG 0.700787 8_1:+76714835 None:intergenic
GTTGTCAAGAGGAACAAAGG+AGG 0.716326 8_1:-76714632 Msa1215460:CDS
CATAGTGCCAAACCTTACGG+AGG 0.733527 8_1:-76714898 Msa1215460:five_prime_UTR
TTTACCGGTAGAAAGTGATG+GGG 0.740060 8_1:+76712621 None:intergenic
GCTAGTGTAACATTGCGTCG+AGG 0.746159 8_1:+76712508 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTATCTATTTTTTTTTTTTT+TGG - chr8_1:76712872-76712891 Msa1215460:CDS 10.0%
!!! AAATTTATTTAGTTTTCAAT+TGG + chr8_1:76713565-76713584 None:intergenic 10.0%
!! CTAAAAATTGTATGTTTATA+GGG - chr8_1:76713313-76713332 Msa1215460:intron 15.0%
!!! AAATTTTATTAGCAGAAATT+CGG - chr8_1:76713579-76713598 Msa1215460:intron 15.0%
!!! TGTTTTTATATTAGTCAATT+TGG - chr8_1:76713936-76713955 Msa1215460:intron 15.0%
!!! TCATCAAATATTTCATATTT+TGG - chr8_1:76714843-76714862 Msa1215460:five_prime_UTR 15.0%
!! TTATTCAAAAATGAAGATGA+TGG + chr8_1:76712448-76712467 None:intergenic 20.0%
!! TCATTATCCTTCATATAATT+CGG + chr8_1:76712732-76712751 None:intergenic 20.0%
!! ATAATGAAACTTTGAATGTT+TGG - chr8_1:76712820-76712839 Msa1215460:CDS 20.0%
!! GCTAAAAATTGTATGTTTAT+AGG - chr8_1:76713312-76713331 Msa1215460:intron 20.0%
!!! TTGTTTACTTTTAACTTTAG+AGG - chr8_1:76713507-76713526 Msa1215460:intron 20.0%
!!! TATTTAGTTTTCAATTGGTA+AGG + chr8_1:76713560-76713579 None:intergenic 20.0%
!! TTCGGAAAACTAAATAAATT+TGG - chr8_1:76713597-76713616 Msa1215460:intron 20.0%
!!! ATTGGTTTAAATTCAAGTTT+AGG - chr8_1:76713767-76713786 Msa1215460:intron 20.0%
!!! CATATTATCTTTTTGTACAT+AGG - chr8_1:76714032-76714051 Msa1215460:intron 20.0%
!!! AATATTTCATATTTTGGTTG+TGG - chr8_1:76714849-76714868 Msa1215460:five_prime_UTR 20.0%
! ATCAAACAAAACCAAATCAA+AGG + chr8_1:76712522-76712541 None:intergenic 25.0%
!!! TGATTTGGTTTTGTTTGATT+TGG - chr8_1:76712523-76712542 Msa1215460:CDS 25.0%
!! TTGGATTTATTCTGTTTCTT+GGG - chr8_1:76712978-76712997 Msa1215460:intron 25.0%
! ATCTCTTACAACAATTTACA+AGG - chr8_1:76713038-76713057 Msa1215460:intron 25.0%
! TTTATGTGTTTATATGTGAG+AGG - chr8_1:76713095-76713114 Msa1215460:intron 25.0%
!!! AACATACAATTTTTAGCACT+AGG + chr8_1:76713309-76713328 None:intergenic 25.0%
! TGTGAGTACAAAAATATTTC+TGG - chr8_1:76713351-76713370 Msa1215460:intron 25.0%
! TAACAATAACAACTAGATCT+TGG - chr8_1:76713741-76713760 Msa1215460:intron 25.0%
! AACAATAACAACTAGATCTT+GGG - chr8_1:76713742-76713761 Msa1215460:intron 25.0%
! CATAGAAACTTTAGTACTAT+TGG + chr8_1:76713813-76713832 None:intergenic 25.0%
!! ACATGTAATTAACTTGAACA+AGG - chr8_1:76713846-76713865 Msa1215460:intron 25.0%
!! CATGTAATTAACTTGAACAA+GGG - chr8_1:76713847-76713866 Msa1215460:intron 25.0%
! ACAAAACTAGAAGCTAATAT+TGG + chr8_1:76713906-76713925 None:intergenic 25.0%
! TATATTAGTCAATTTGGATG+AGG - chr8_1:76713942-76713961 Msa1215460:intron 25.0%
!! TTTGGATGAGGATTTTTATT+AGG - chr8_1:76713954-76713973 Msa1215460:intron 25.0%
!! TTAATAATTTGAGTTACCGA+CGG - chr8_1:76714133-76714152 Msa1215460:intron 25.0%
!!! AGAAGTCGATTTCATTTTTT+CGG + chr8_1:76714169-76714188 None:intergenic 25.0%
! TTTAAACCATCAATTACCAT+TGG - chr8_1:76714288-76714307 Msa1215460:intron 25.0%
! AATCTATTCTTAACAGAAAC+AGG + chr8_1:76714444-76714463 None:intergenic 25.0%
! CTGTTAAGAATAGATTATGT+CGG - chr8_1:76714448-76714467 Msa1215460:CDS 25.0%
! CAGCCATCATAAATTAGTTT+GGG - chr8_1:76712273-76712292 Msa1215460:three_prime_UTR 30.0%
AGACCCAAACTAATTTATGA+TGG + chr8_1:76712279-76712298 None:intergenic 30.0%
AACACAAACAACCTTTGATT+TGG - chr8_1:76712508-76712527 Msa1215460:CDS 30.0%
CGACATCTTTATATTATCCT+TGG + chr8_1:76712652-76712671 None:intergenic 30.0%
ATATAAAGATGTCGCATGAT+CGG - chr8_1:76712658-76712677 Msa1215460:CDS 30.0%
!!! TTTTTGGTTTTCATTCTGTC+TGG - chr8_1:76712888-76712907 Msa1215460:CDS 30.0%
ATCCAACTAAATGTCTGAAA+AGG + chr8_1:76712964-76712983 None:intergenic 30.0%
! GTTGGATTTATTCTGTTTCT+TGG - chr8_1:76712977-76712996 Msa1215460:intron 30.0%
AAAGAATAAGAGAACCAATC+TGG - chr8_1:76713144-76713163 Msa1215460:CDS 30.0%
AAGCAAACATGACATGAAAA+TGG - chr8_1:76713663-76713682 Msa1215460:intron 30.0%
ATGACATGAAAATGGTTTCT+TGG - chr8_1:76713671-76713690 Msa1215460:intron 30.0%
ACAATAACAACTAGATCTTG+GGG - chr8_1:76713743-76713762 Msa1215460:intron 30.0%
GCAAAATTAGTTGAGAATTG+TGG + chr8_1:76714013-76714032 None:intergenic 30.0%
TGACAATGTAAGAATTTGCA+GGG - chr8_1:76714247-76714266 Msa1215460:CDS 30.0%
! CCTTGCTATAACAAATTCTA+AGG + chr8_1:76714329-76714348 None:intergenic 30.0%
!! CCTTAGAATTTGTTATAGCA+AGG - chr8_1:76714326-76714345 Msa1215460:intron 30.0%
!! TAGAATTTGTTATAGCAAGG+TGG - chr8_1:76714329-76714348 Msa1215460:intron 30.0%
GGAAAACATAGTAAATCTGA+GGG + chr8_1:76714504-76714523 None:intergenic 30.0%
AGGAAAACATAGTAAATCTG+AGG + chr8_1:76714505-76714524 None:intergenic 30.0%
!!! TTTCATATTTTGGTTGTGGT+TGG - chr8_1:76714853-76714872 Msa1215460:five_prime_UTR 30.0%
CTACACTTAAAATGCTAGAA+TGG - chr8_1:76714896-76714915 Msa1215460:five_prime_UTR 30.0%
ATACATAGTGCCAAACCTTA+CGG - chr8_1:76712322-76712341 Msa1215460:CDS 35.0%
!! TGTTGATGATGGAGTAATGA+GGG + chr8_1:76712390-76712409 None:intergenic 35.0%
!! ATGTTGATGATGGAGTAATG+AGG + chr8_1:76712391-76712410 None:intergenic 35.0%
GAGCATATATTTCTCTGACT+TGG + chr8_1:76712426-76712445 None:intergenic 35.0%
CACAATAGTTCATCTCTGTT+TGG + chr8_1:76712560-76712579 None:intergenic 35.0%
AGACAAATTGTGTTGTCAAG+AGG - chr8_1:76712580-76712599 Msa1215460:CDS 35.0%
! TTACGCTCTTGTGTCTTTTA+TGG - chr8_1:76712701-76712720 Msa1215460:CDS 35.0%
TACGTTATTACAGAGCCAAT+TGG - chr8_1:76712760-76712779 Msa1215460:CDS 35.0%
CTGAAGAAAGCTTCTTTCAA+AGG + chr8_1:76712786-76712805 None:intergenic 35.0%
!! TCTGTCTGGTGTTTTTGATT+AGG - chr8_1:76712902-76712921 Msa1215460:CDS 35.0%
! TGCCTTTTCAGACATTTAGT+TGG - chr8_1:76712959-76712978 Msa1215460:intron 35.0%
! ATGCTTTCTGAGAAATGTTG+AGG + chr8_1:76713172-76713191 None:intergenic 35.0%
ACACCTTATACAATACACTC+TGG - chr8_1:76713283-76713302 Msa1215460:intron 35.0%
!!! ACTTTTAACTTTAGAGGAGC+TGG - chr8_1:76713513-76713532 Msa1215460:intron 35.0%
TTCAATTGGTAAGGTTACCA+AGG + chr8_1:76713551-76713570 None:intergenic 35.0%
!!! TATTGGTTTTGTCAACAGGA+CGG + chr8_1:76713796-76713815 None:intergenic 35.0%
!!! GTACTATTGGTTTTGTCAAC+AGG + chr8_1:76713800-76713819 None:intergenic 35.0%
!! AGTTTCTATGCAGTTTGTTG+TGG - chr8_1:76713823-76713842 Msa1215460:intron 35.0%
AACTGTCTAGCTAATAGCAT+AGG - chr8_1:76713979-76713998 Msa1215460:intron 35.0%
ACTGTCTAGCTAATAGCATA+GGG - chr8_1:76713980-76713999 Msa1215460:intron 35.0%
TTCTGATAGCATCAAAGTTC+TGG - chr8_1:76714084-76714103 Msa1215460:intron 35.0%
AACGCTTAGCAACGAAATTA+TGG - chr8_1:76714204-76714223 Msa1215460:CDS 35.0%
CTTAGCAACGAAATTATGGA+CGG - chr8_1:76714208-76714227 Msa1215460:CDS 35.0%
CTGACAATGTAAGAATTTGC+AGG - chr8_1:76714246-76714265 Msa1215460:CDS 35.0%
ATGTAAGAATTTGCAGGGAA+CGG - chr8_1:76714252-76714271 Msa1215460:CDS 35.0%
CAGAAACAGGTTTAAGTCAT+GGG + chr8_1:76714431-76714450 None:intergenic 35.0%
ACAGAAACAGGTTTAAGTCA+TGG + chr8_1:76714432-76714451 None:intergenic 35.0%
! TTTTCCTATGTCAACCTCAT+TGG - chr8_1:76714518-76714537 Msa1215460:CDS 35.0%
AGCTATGTTGTTCTGTTCAA+TGG - chr8_1:76714639-76714658 Msa1215460:CDS 35.0%
GCTATGTTGTTCTGTTCAAT+GGG - chr8_1:76714640-76714659 Msa1215460:CDS 35.0%
! TTCTGTTCAATGGGACTTTT+CGG - chr8_1:76714649-76714668 Msa1215460:CDS 35.0%
TTCATGCTTGTGTTGCAAAT+TGG - chr8_1:76714686-76714705 Msa1215460:five_prime_UTR 35.0%
TCATGCTTGTGTTGCAAATT+GGG - chr8_1:76714687-76714706 Msa1215460:five_prime_UTR 35.0%
ACTAGCATATGAATGTGTCA+TGG - chr8_1:76714732-76714751 Msa1215460:five_prime_UTR 35.0%
ATGGCATTGTCTTACTTGAT+AGG - chr8_1:76714751-76714770 Msa1215460:five_prime_UTR 35.0%
TGGCATTGTCTTACTTGATA+GGG - chr8_1:76714752-76714771 Msa1215460:five_prime_UTR 35.0%
GTGTGACAAGGCTTAAATTA+AGG - chr8_1:76714933-76714952 Msa1215460:five_prime_UTR 35.0%
AGGTTTGGCACTATGTATGT+GGG + chr8_1:76712320-76712339 None:intergenic 40.0%
AAGGTTTGGCACTATGTATG+TGG + chr8_1:76712321-76712340 None:intergenic 40.0%
! GTGTGGCTAAATGTTGATGA+TGG + chr8_1:76712401-76712420 None:intergenic 40.0%
!! ATTTCTCTGACTTGGTTGTG+TGG + chr8_1:76712418-76712437 None:intergenic 40.0%
TGTGTTGTCAAGAGGAACAA+AGG - chr8_1:76712588-76712607 Msa1215460:CDS 40.0%
CTTGGACTGTGTAAAAGACA+AGG + chr8_1:76712634-76712653 None:intergenic 40.0%
! CTTGTCTTTTACACAGTCCA+AGG - chr8_1:76712632-76712651 Msa1215460:CDS 40.0%
GGAGCTACCGAATTATATGA+AGG - chr8_1:76712722-76712741 Msa1215460:CDS 40.0%
AGCTTCTTTCAAAGGCCAAT+TGG + chr8_1:76712778-76712797 None:intergenic 40.0%
CTTTCTTCAGTCTCTTTCGT+TGG - chr8_1:76712796-76712815 Msa1215460:CDS 40.0%
CACATGAGGCTTCATCAAAT+TGG + chr8_1:76713014-76713033 None:intergenic 40.0%
CAATTTGATGAAGCCTCATG+TGG - chr8_1:76713012-76713031 Msa1215460:intron 40.0%
GAAATGTTGAGGAACCAGAT+TGG + chr8_1:76713161-76713180 None:intergenic 40.0%
AGGCCAGAGTGTATTGTATA+AGG + chr8_1:76713289-76713308 None:intergenic 40.0%
!! GCTGGATCTCTAAAATACCT+TGG - chr8_1:76713531-76713550 Msa1215460:intron 40.0%
ACAACTAGATCTTGGGGAAT+TGG - chr8_1:76713749-76713768 Msa1215460:intron 40.0%
GTACATAGGTCTATCTCTTC+AGG - chr8_1:76714046-76714065 Msa1215460:intron 40.0%
TTTCGGTCTCTAATTTCCGT+CGG + chr8_1:76714152-76714171 None:intergenic 40.0%
CCATTGCCAATGGTAATTGA+TGG + chr8_1:76714297-76714316 None:intergenic 40.0%
CCATCAATTACCATTGGCAA+TGG - chr8_1:76714294-76714313 Msa1215460:intron 40.0%
GATTCAACTTCCATTGCCAA+TGG + chr8_1:76714307-76714326 None:intergenic 40.0%
TGGCCATTCTTTGTCTTCTT+AGG - chr8_1:76714349-76714368 Msa1215460:intron 40.0%
CCACCTAAGAAGACAAAGAA+TGG + chr8_1:76714355-76714374 None:intergenic 40.0%
CCATTCTTTGTCTTCTTAGG+TGG - chr8_1:76714352-76714371 Msa1215460:intron 40.0%
GTAAATCTGAGGGAAGAATG+AGG + chr8_1:76714494-76714513 None:intergenic 40.0%
TACTATGGTGAATAGTCCCA+TGG + chr8_1:76714572-76714591 None:intergenic 40.0%
TGGGGAAAGCAATGTTACTA+TGG + chr8_1:76714587-76714606 None:intergenic 40.0%
TTTACCGGTAGAAAGTGATG+GGG + chr8_1:76714605-76714624 None:intergenic 40.0%
GTTTACCGGTAGAAAGTGAT+GGG + chr8_1:76714606-76714625 None:intergenic 40.0%
TGTTTACCGGTAGAAAGTGA+TGG + chr8_1:76714607-76714626 None:intergenic 40.0%
CATGCTTGTGTTGCAAATTG+GGG - chr8_1:76714688-76714707 Msa1215460:five_prime_UTR 40.0%
! TAGAATGGCGTGACACTTTT+GGG - chr8_1:76714911-76714930 Msa1215460:five_prime_UTR 40.0%
GGTTTGGCACTATGTATGTG+GGG + chr8_1:76712319-76712338 None:intergenic 45.0%
TGTCCCTTAAATGCTGCAGT+TGG - chr8_1:76712354-76712373 Msa1215460:CDS 45.0%
GCACCAACTGCAGCATTTAA+GGG + chr8_1:76712360-76712379 None:intergenic 45.0%
TGCACCAACTGCAGCATTTA+AGG + chr8_1:76712361-76712380 None:intergenic 45.0%
GTTGTCAAGAGGAACAAAGG+AGG - chr8_1:76712591-76712610 Msa1215460:CDS 45.0%
ATGTCGCATGATCGGAACAA+AGG - chr8_1:76712666-76712685 Msa1215460:CDS 45.0%
!! TCTGTTTCTTGGGTTGACCT+TGG - chr8_1:76712988-76713007 Msa1215460:intron 45.0%
AGGCTTCATCAAATTGGCCA+AGG + chr8_1:76713008-76713027 None:intergenic 45.0%
TTTGATGAAGCCTCATGTGG+TGG - chr8_1:76713015-76713034 Msa1215460:intron 45.0%
TGTAAGAGATCCACCACATG+AGG + chr8_1:76713028-76713047 None:intergenic 45.0%
CATAGGTCTATCTCTTCAGG+TGG - chr8_1:76714049-76714068 Msa1215460:intron 45.0%
CATGGGAGTGACATGAAAAG+AGG + chr8_1:76714414-76714433 None:intergenic 45.0%
GTTGCCAATGAGGTTGACAT+AGG + chr8_1:76714525-76714544 None:intergenic 45.0%
AGGTAGATGAGTTGCCAATG+AGG + chr8_1:76714535-76714554 None:intergenic 45.0%
TAGTCCCATGGCTGTAATAC+CGG + chr8_1:76714560-76714579 None:intergenic 45.0%
CTTTCCCCATCACTTTCTAC+CGG - chr8_1:76714598-76714617 Msa1215460:CDS 45.0%
ACAACATAGCTCGAAATGCG+CGG + chr8_1:76714630-76714649 None:intergenic 45.0%
GGACTTTGTTCTATGTGACG+CGG - chr8_1:76714773-76714792 Msa1215460:five_prime_UTR 45.0%
CCTGCTAAATCAAACCAACC+AGG + chr8_1:76714817-76714836 None:intergenic 45.0%
!! CCTGGTTGGTTTGATTTAGC+AGG - chr8_1:76714814-76714833 Msa1215460:five_prime_UTR 45.0%
! CTAGAATGGCGTGACACTTT+TGG - chr8_1:76714910-76714929 Msa1215460:five_prime_UTR 45.0%
!! TGACACTTTTGGGTGTGACA+AGG - chr8_1:76714921-76714940 Msa1215460:five_prime_UTR 45.0%
CATAGTGCCAAACCTTACGG+AGG - chr8_1:76712325-76712344 Msa1215460:CDS 50.0%
ACACTCTCCTCCGTAAGGTT+TGG + chr8_1:76712335-76712354 None:intergenic 50.0%
ATATGCCAGCTGTTACCTGC+AGG - chr8_1:76713190-76713209 Msa1215460:intron 50.0%
AATGACCTGCAGGTAACAGC+TGG + chr8_1:76713198-76713217 None:intergenic 50.0%
AGCTGTTACCTGCAGGTCAT+TGG - chr8_1:76713197-76713216 Msa1215460:intron 50.0%
ACACAACACCAATGACCTGC+AGG + chr8_1:76713208-76713227 None:intergenic 50.0%
GGGAGTGACATGAAAAGAGG+TGG + chr8_1:76714411-76714430 None:intergenic 50.0%
TGGCAACTCATCTACCTAGC+CGG - chr8_1:76714538-76714557 Msa1215460:CDS 50.0%
CTAGCCGGTATTACAGCCAT+GGG - chr8_1:76714553-76714572 Msa1215460:CDS 50.0%
TCGAAATGCGCGGTGTTTAC+CGG + chr8_1:76714620-76714639 None:intergenic 50.0%
GCAACACAAGCATGAACTGC+AGG + chr8_1:76714682-76714701 None:intergenic 50.0%
GCTAGTGTAACATTGCGTCG+AGG + chr8_1:76714718-76714737 None:intergenic 50.0%
GTTCTATGTGACGCGGATAC+CGG - chr8_1:76714780-76714799 Msa1215460:five_prime_UTR 50.0%
GGTGGCAAATGCTGCTAGAG+AGG + chr8_1:76714393-76714412 None:intergenic 55.0%
! CCATGGCTGTAATACCGGCT+AGG + chr8_1:76714555-76714574 None:intergenic 55.0%
CCTAGCCGGTATTACAGCCA+TGG - chr8_1:76714552-76714571 Msa1215460:CDS 55.0%
TGACGCGGATACCGGAAAGA+TGG - chr8_1:76714788-76714807 Msa1215460:five_prime_UTR 55.0%
ATACCGGAAAGATGGAGGCC+TGG - chr8_1:76714796-76714815 Msa1215460:five_prime_UTR 55.0%
CAACCAGGCCTCCATCTTTC+CGG + chr8_1:76714802-76714821 None:intergenic 55.0%
GGGACACACTCTCCTCCGTA+AGG + chr8_1:76712340-76712359 None:intergenic 60.0%
CGCGGATACCGGAAAGATGG+AGG - chr8_1:76714791-76714810 Msa1215460:five_prime_UTR 60.0%
CGGAAAGATGGAGGCCTGGT+TGG - chr8_1:76714800-76714819 Msa1215460:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 76712273 76714972 76712273 ID=Msa1215460;Name=Msa1215460
chr8_1 mRNA 76712273 76714972 76712273 ID=Msa1215460-mRNA-1;Parent=Msa1215460;Name=Msa1215460-mRNA-1;_AED=0.04;_eAED=0.04;_QI=294|1|1|1|1|1|4|24|366
chr8_1 exon 76712273 76712977 76712273 ID=Msa1215460-mRNA-1:exon:26394;Parent=Msa1215460-mRNA-1
chr8_1 exon 76713140 76713191 76713140 ID=Msa1215460-mRNA-1:exon:26393;Parent=Msa1215460-mRNA-1
chr8_1 exon 76714186 76714275 76714186 ID=Msa1215460-mRNA-1:exon:26392;Parent=Msa1215460-mRNA-1
chr8_1 exon 76714401 76714972 76714401 ID=Msa1215460-mRNA-1:exon:26391;Parent=Msa1215460-mRNA-1
chr8_1 five_prime_UTR 76714679 76714972 76714679 ID=Msa1215460-mRNA-1:five_prime_utr;Parent=Msa1215460-mRNA-1
chr8_1 CDS 76714401 76714678 76714401 ID=Msa1215460-mRNA-1:cds;Parent=Msa1215460-mRNA-1
chr8_1 CDS 76714186 76714275 76714186 ID=Msa1215460-mRNA-1:cds;Parent=Msa1215460-mRNA-1
chr8_1 CDS 76713140 76713191 76713140 ID=Msa1215460-mRNA-1:cds;Parent=Msa1215460-mRNA-1
chr8_1 CDS 76712297 76712977 76712297 ID=Msa1215460-mRNA-1:cds;Parent=Msa1215460-mRNA-1
chr8_1 three_prime_UTR 76712273 76712296 76712273 ID=Msa1215460-mRNA-1:three_prime_utr;Parent=Msa1215460-mRNA-1
Gene Sequence

>Msa1215460

ATGAACTATTGTGAGACAAATTGTGTTGTCAAGAGGAACAAAGGAGGTAAAGAAACTGATGAAACCTTGTCTTTTACACAGTCCAAGGATAATATAAAGATGTCGCATGATCGGAACAAAGGAAAACAACAACGTTACGCTCTTGTGTCTTTTATGGAGCTACCGAATTATATGAAGGATAATGAATATATTTTACGTTATTACAGAGCCAATTGGCCTTTGAAAGAAGCTTTCTTCAGTCTCTTTCGTTGGCATAATGAAACTTTGAATGTTTGGACACATTTAGTTGGATTTATTCTGTTTCTTGGGTTGACCTTGGCCAATTTGATGAAGCCTCATGTGGTGGATCTCTTACAACAATTTACAAGGTCTATCTCTTCAGGTGGTGAAAAAAATGTTTCTGATAGCATCAAAGTTCTGGGAACGGCGTTACTCTTTGATTTAAACCATCAATTACCATTGGCAATGGAAGTTGAATCCTTAGAATTTGTTATAGCAAGGTGGCCATTCTTTGTCTTCTTAGGTGGTTCAATGTTCTGTCTCCTCTCTAGCAGCATTTGCCACCTCTTTTCATGTCACTCCCATGACTTAAACCTGTTTCTGTTAAGAATAGATTATGTCGGAATCGCTGTCATGATCATAACCTCATTCTTCCCTCAGATTTACTATGTTTTCCTATGTCAACCTCATTGGCAACTCATCTACCTAGCCGGTATTACAGCCATGGGACTATTCACCATAGTAACATTGCTTTCCCCATCACTTTCTACCGGTAAACACCGCGCATTTCGAGCTATGTTGTTCTGTTCAATGGGACTTTTCGGCATTGTGCCTGCAGTTCATGCTTGTGTTGCAAATTGGGGAAATCCTCGACGCAATGTTACACTAGCATATGAATGTGTCATGGCATTGTCTTACTTGATAGGGACTTTGTTCTATGTGACGCGGATACCGGAAAGATGGAGGCCTGGTTGGTTTGATTTAGCAGGACATAGTCATCAAATATTTCATATTTTGGTTGTGGTTGGTGCATTATCACATTATGCAGCTACACTTAAAATGCTAGAATGGCGTGACACTTTTGGGTGTGACAAGGCTTAA
Protein sequence

>Msa1215460

MNYCETNCVVKRNKGGKETDETLSFTQSKDNIKMSHDRNKGKQQRYALVSFMELPNYMKDNEYILRYYRANWPLKEAFFSLFRWHNETLNVWTHLVGFILFLGLTLANLMKPHVVDLLQQFTRSISSGGEKNVSDSIKVLGTALLFDLNHQLPLAMEVESLEFVIARWPFFVFLGGSMFCLLSSSICHLFSCHSHDLNLFLLRIDYVGIAVMIITSFFPQIYYVFLCQPHWQLIYLAGITAMGLFTIVTLLSPSLSTGKHRAFRAMLFCSMGLFGIVPAVHACVANWGNPRRNVTLAYECVMALSYLIGTLFYVTRIPERWRPGWFDLAGHSHQIFHILVVVGALSHYAATLKMLEWRDTFGCDKA*