AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0485220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485220 MtrunA17_Chr4g0063571 100.000 128 0 0 1 128 1 128 1.30e-91 260
Msa0485220 MtrunA17_Chr2g0307081 97.656 128 3 0 1 128 1 128 3.04e-89 254
Msa0485220 MtrunA17_Chr4g0061981 34.483 116 75 1 4 118 17 132 1.14e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0485220 AT4G22380.1 93.750 128 8 0 1 128 1 128 1.17e-76 223
Msa0485220 AT5G20160.1 92.969 128 9 0 1 128 1 128 4.97e-76 221
Msa0485220 AT4G12600.1 89.844 128 13 0 1 128 1 128 1.31e-72 213
Msa0485220 AT5G20160.2 74.375 160 9 1 1 128 1 160 3.95e-69 205
Msa0485220 AT4G12600.2 55.738 183 26 2 1 128 1 183 2.20e-49 156
Msa0485220 AT5G20160.3 65.741 108 5 1 1 76 1 108 1.15e-31 108
Msa0485220 AT5G08180.2 35.938 128 73 1 8 126 24 151 3.79e-21 83.6
Msa0485220 AT5G08180.1 35.938 128 73 1 8 126 24 151 3.79e-21 83.6

Find 50 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAGCGGCTAGGGTTTA+TGG 0.198145 3_4:-2777950 None:intergenic
TAACTATAAGCAACTCAAAA+AGG 0.236397 3_4:+2778203 Msa0485220:CDS
GAAGACTTTATATATATAAT+TGG 0.242176 3_4:-2777893 None:intergenic
TATTTCTGAATTTGTTGTTA+TGG 0.302213 3_4:+2778346 Msa0485220:CDS
TTGTTGAACAAGATCCATTA+TGG 0.342840 3_4:-2778175 None:intergenic
TCCGTTACTTGCTGAGGATA+AGG 0.383122 3_4:+2778406 Msa0485220:CDS
AGCTACTAAGACCTTGAATA+GGG 0.384258 3_4:+2778322 Msa0485220:intron
TCAGCCAAAGGGTATGCCTT+AGG 0.396497 3_4:-2778135 None:intergenic
AGTTGTGCTGAAAGCGTCTT+CGG 0.405427 3_4:+2779457 Msa0485220:three_prime_UTR
GCGTCTTCGGAGGCCTTGAC+TGG 0.409377 3_4:+2779470 Msa0485220:three_prime_UTR
CAGCTACTAAGACCTTGAAT+AGG 0.416543 3_4:+2778321 Msa0485220:intron
TTCAGAAATACCCCTATTCA+AGG 0.432400 3_4:-2778333 None:intergenic
GTCTTCGTTTCTTATAATAA+CGG 0.434891 3_4:+2777910 Msa0485220:five_prime_UTR
AACGGTACCATTGTTAATTG+AGG 0.440521 3_4:-2777987 None:intergenic
TAACCCTAAGGCATACCCTT+TGG 0.467615 3_4:+2778131 Msa0485220:CDS
TACCACACAATAACAACCTT+CGG 0.470397 3_4:-2779551 None:intergenic
TCAGCTAACAATCACCATAA+TGG 0.473714 3_4:+2778161 Msa0485220:CDS
CAGCCAAAGGGTATGCCTTA+GGG 0.474338 3_4:-2778134 None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG 0.475355 3_4:-2778400 None:intergenic
TGTGTTCGTCACATCAAAAC+AGG 0.479560 3_4:+2779164 Msa0485220:CDS
CCATCTTCCGTTACTTGCTG+AGG 0.512018 3_4:+2778400 Msa0485220:CDS
CGCCGAAGGTTGTTATTGTG+TGG 0.518320 3_4:+2779549 Msa0485220:three_prime_UTR
GTCACATCAAAACAGGCACT+TGG 0.523031 3_4:+2779171 Msa0485220:CDS
TTATCCGTATGATGGTATGA+TGG 0.527837 3_4:+2779426 Msa0485220:three_prime_UTR
AAACTTCATTATCCGTATGA+TGG 0.529098 3_4:+2779418 Msa0485220:three_prime_UTR
AGAACAAGCAATCACTGGTC+GGG 0.539786 3_4:-2779211 None:intergenic
GTAACACTCATGCATCATAC+AGG 0.540573 3_4:-2779736 None:intergenic
TATCCGTATGATGGTATGAT+GGG 0.540926 3_4:+2779427 Msa0485220:three_prime_UTR
GAAGAGAATGAAAGCGGCTA+GGG 0.556939 3_4:-2777957 None:intergenic
GTCACAGAACAAGCAATCAC+TGG 0.562045 3_4:-2779216 None:intergenic
AAACTGAATTTACTAGTCAA+AGG 0.565236 3_4:-2779638 None:intergenic
CGAAGAGAATGAAAGCGGCT+AGG 0.565826 3_4:-2777958 None:intergenic
TTTGATGTGACGAACACATA+GGG 0.566367 3_4:-2779159 None:intergenic
CAGGCACTTGGGCGTGCTTG+TGG 0.575874 3_4:+2779183 Msa0485220:CDS
CACAACAAGTCGTCCAGTCA+AGG 0.578850 3_4:-2779483 None:intergenic
TTAGCTGAGCATCAGCCAAA+GGG 0.590874 3_4:-2778146 None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG 0.593594 3_4:-2778407 None:intergenic
AAGCCCATCATACCATCATA+CGG 0.597239 3_4:-2779430 None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG 0.598758 3_4:-2778147 None:intergenic
GTCTCAGATTCAGCAACTCA+AGG 0.599622 3_4:+2779263 Msa0485220:CDS
GTTCAATCCTCAATTAACAA+TGG 0.604789 3_4:+2777980 Msa0485220:exon
TGTTCTGTGACAACTAATGA+AGG 0.613805 3_4:+2779228 Msa0485220:CDS
CAGAACAAGCAATCACTGGT+CGG 0.618383 3_4:-2779212 None:intergenic
TGGAGAAGCAGTTAACCCTA+AGG 0.621032 3_4:+2778119 Msa0485220:CDS
TCACATCAAAACAGGCACTT+GGG 0.623134 3_4:+2779172 Msa0485220:CDS
TGTGCTGAAAGCGTCTTCGG+AGG 0.641398 3_4:+2779460 Msa0485220:three_prime_UTR
AGATGGAGAAGAATCTCAAG+AGG 0.644746 3_4:-2778383 None:intergenic
GCTACTAAGACCTTGAATAG+GGG 0.659558 3_4:+2778323 Msa0485220:intron
TTGAACGAAGAGAATGAAAG+CGG 0.665410 3_4:-2777963 None:intergenic
CACACAATAACAACCTTCGG+CGG 0.714102 3_4:-2779548 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGAAATAAGAAATTA+GGG - chr3_4:2778245-2778264 None:intergenic 10.0%
!! AAAAAATGAAATAAGAAATT+AGG - chr3_4:2778246-2778265 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG + chr3_4:2778710-2778729 Msa0485220:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG - chr3_4:2779064-2779083 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG + chr3_4:2779068-2779087 Msa0485220:intron 10.0%
!! AAAAATGAAATAAGAAATTA+GGG - chr3_4:2778245-2778264 None:intergenic 10.0%
!! AAAAAATGAAATAAGAAATT+AGG - chr3_4:2778246-2778265 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG + chr3_4:2778710-2778729 Msa0485220:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG - chr3_4:2779064-2779083 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG + chr3_4:2779068-2779087 Msa0485220:intron 10.0%
!! GAAGACTTTATATATATAAT+TGG - chr3_4:2777896-2777915 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG - chr3_4:2778008-2778027 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG - chr3_4:2779530-2779549 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG - chr3_4:2779605-2779624 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG + chr3_4:2779767-2779786 Msa0485220:three_prime_UTR 15.0%
!! CTTTATATATATAATTGGTT+TGG - chr3_4:2777891-2777910 None:intergenic 15.0%
!! GAAGACTTTATATATATAAT+TGG - chr3_4:2777896-2777915 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG - chr3_4:2778008-2778027 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG - chr3_4:2779530-2779549 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG - chr3_4:2779605-2779624 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG + chr3_4:2779767-2779786 Msa0485220:three_prime_UTR 15.0%
!! CAAATTAAGAGTTAAACATA+GGG - chr3_4:2778035-2778054 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG - chr3_4:2778036-2778055 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG + chr3_4:2778346-2778365 Msa0485220:CDS 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG + chr3_4:2778527-2778546 Msa0485220:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG - chr3_4:2778699-2778718 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG + chr3_4:2779357-2779376 Msa0485220:intron 20.0%
!! CAAATTAAGAGTTAAACATA+GGG - chr3_4:2778035-2778054 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG - chr3_4:2778036-2778055 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG + chr3_4:2778346-2778365 Msa0485220:CDS 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG + chr3_4:2778527-2778546 Msa0485220:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG - chr3_4:2778699-2778718 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG + chr3_4:2779357-2779376 Msa0485220:intron 20.0%
! GTCTTCGTTTCTTATAATAA+CGG + chr3_4:2777910-2777929 Msa0485220:five_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG + chr3_4:2778203-2778222 Msa0485220:CDS 25.0%
! AACTATAAGCAACTCAAAAA+GGG + chr3_4:2778204-2778223 Msa0485220:CDS 25.0%
!! TTGTCCTTTGATTATTGATT+TGG + chr3_4:2778280-2778299 Msa0485220:intron 25.0%
! CAAACCAAATCAATAATCAA+AGG - chr3_4:2778287-2778306 None:intergenic 25.0%
! AGTAATATTACTTGAAGACA+TGG - chr3_4:2778942-2778961 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG - chr3_4:2779055-2779074 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG + chr3_4:2779361-2779380 Msa0485220:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG + chr3_4:2779593-2779612 Msa0485220:three_prime_UTR 25.0%
! AAACTGAATTTACTAGTCAA+AGG - chr3_4:2779641-2779660 None:intergenic 25.0%
! GTCTTCGTTTCTTATAATAA+CGG + chr3_4:2777910-2777929 Msa0485220:five_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG + chr3_4:2778203-2778222 Msa0485220:CDS 25.0%
! AACTATAAGCAACTCAAAAA+GGG + chr3_4:2778204-2778223 Msa0485220:CDS 25.0%
!! TTGTCCTTTGATTATTGATT+TGG + chr3_4:2778280-2778299 Msa0485220:intron 25.0%
! CAAACCAAATCAATAATCAA+AGG - chr3_4:2778287-2778306 None:intergenic 25.0%
! AGTAATATTACTTGAAGACA+TGG - chr3_4:2778942-2778961 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG - chr3_4:2779055-2779074 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG + chr3_4:2779361-2779380 Msa0485220:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG + chr3_4:2779593-2779612 Msa0485220:three_prime_UTR 25.0%
! AAACTGAATTTACTAGTCAA+AGG - chr3_4:2779641-2779660 None:intergenic 25.0%
GTTCAATCCTCAATTAACAA+TGG + chr3_4:2777980-2777999 Msa0485220:exon 30.0%
ATGATGAAAAATTTGCAGAC+TGG + chr3_4:2778099-2778118 Msa0485220:intron 30.0%
TTGTTGAACAAGATCCATTA+TGG - chr3_4:2778178-2778197 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG - chr3_4:2778627-2778646 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG + chr3_4:2778632-2778651 Msa0485220:intron 30.0%
TTTGTATCCATACAGATGTT+TGG + chr3_4:2778663-2778682 Msa0485220:intron 30.0%
TTGTATCCATACAGATGTTT+GGG + chr3_4:2778664-2778683 Msa0485220:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG + chr3_4:2778759-2778778 Msa0485220:intron 30.0%
AAACTTCATTATCCGTATGA+TGG + chr3_4:2779418-2779437 Msa0485220:three_prime_UTR 30.0%
GTTCAATCCTCAATTAACAA+TGG + chr3_4:2777980-2777999 Msa0485220:exon 30.0%
ATGATGAAAAATTTGCAGAC+TGG + chr3_4:2778099-2778118 Msa0485220:intron 30.0%
TTGTTGAACAAGATCCATTA+TGG - chr3_4:2778178-2778197 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG - chr3_4:2778627-2778646 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG + chr3_4:2778632-2778651 Msa0485220:intron 30.0%
TTTGTATCCATACAGATGTT+TGG + chr3_4:2778663-2778682 Msa0485220:intron 30.0%
TTGTATCCATACAGATGTTT+GGG + chr3_4:2778664-2778683 Msa0485220:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG + chr3_4:2778759-2778778 Msa0485220:intron 30.0%
AAACTTCATTATCCGTATGA+TGG + chr3_4:2779418-2779437 Msa0485220:three_prime_UTR 30.0%
TTGAACGAAGAGAATGAAAG+CGG - chr3_4:2777966-2777985 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG - chr3_4:2777990-2778009 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG + chr3_4:2778161-2778180 Msa0485220:CDS 35.0%
AGCTACTAAGACCTTGAATA+GGG + chr3_4:2778322-2778341 Msa0485220:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG - chr3_4:2778336-2778355 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG + chr3_4:2778415-2778434 Msa0485220:intron 35.0%
GCTGAGGATAAGGTATATTA+GGG + chr3_4:2778416-2778435 Msa0485220:intron 35.0%
TGCTATGCATAACAAATAGC+GGG - chr3_4:2778626-2778645 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG + chr3_4:2778752-2778771 Msa0485220:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG + chr3_4:2778753-2778772 Msa0485220:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG - chr3_4:2778835-2778854 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG + chr3_4:2778833-2778852 Msa0485220:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG + chr3_4:2778866-2778885 Msa0485220:intron 35.0%
CATGGTTTATCACACTTGTT+TGG - chr3_4:2778924-2778943 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG - chr3_4:2779162-2779181 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG - chr3_4:2779163-2779182 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG + chr3_4:2779228-2779247 Msa0485220:CDS 35.0%
! TGAATATTTGGTTGGTATGC+AGG + chr3_4:2779369-2779388 Msa0485220:intron 35.0%
TTATCCGTATGATGGTATGA+TGG + chr3_4:2779426-2779445 Msa0485220:three_prime_UTR 35.0%
TATCCGTATGATGGTATGAT+GGG + chr3_4:2779427-2779446 Msa0485220:three_prime_UTR 35.0%
! AATTTTCTTTTTACCGCCGA+AGG + chr3_4:2779535-2779554 Msa0485220:three_prime_UTR 35.0%
TACCACACAATAACAACCTT+CGG - chr3_4:2779554-2779573 None:intergenic 35.0%
TTGAACGAAGAGAATGAAAG+CGG - chr3_4:2777966-2777985 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG - chr3_4:2777990-2778009 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG + chr3_4:2778161-2778180 Msa0485220:CDS 35.0%
AGCTACTAAGACCTTGAATA+GGG + chr3_4:2778322-2778341 Msa0485220:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG - chr3_4:2778336-2778355 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG + chr3_4:2778415-2778434 Msa0485220:intron 35.0%
GCTGAGGATAAGGTATATTA+GGG + chr3_4:2778416-2778435 Msa0485220:intron 35.0%
TGCTATGCATAACAAATAGC+GGG - chr3_4:2778626-2778645 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG + chr3_4:2778752-2778771 Msa0485220:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG + chr3_4:2778753-2778772 Msa0485220:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG - chr3_4:2778835-2778854 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG + chr3_4:2778833-2778852 Msa0485220:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG + chr3_4:2778866-2778885 Msa0485220:intron 35.0%
CATGGTTTATCACACTTGTT+TGG - chr3_4:2778924-2778943 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG - chr3_4:2779162-2779181 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG - chr3_4:2779163-2779182 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG + chr3_4:2779228-2779247 Msa0485220:CDS 35.0%
! TGAATATTTGGTTGGTATGC+AGG + chr3_4:2779369-2779388 Msa0485220:intron 35.0%
TTATCCGTATGATGGTATGA+TGG + chr3_4:2779426-2779445 Msa0485220:three_prime_UTR 35.0%
TATCCGTATGATGGTATGAT+GGG + chr3_4:2779427-2779446 Msa0485220:three_prime_UTR 35.0%
! AATTTTCTTTTTACCGCCGA+AGG + chr3_4:2779535-2779554 Msa0485220:three_prime_UTR 35.0%
TACCACACAATAACAACCTT+CGG - chr3_4:2779554-2779573 None:intergenic 35.0%
!! CTCAAAAAGGGTGCTAATGA+AGG + chr3_4:2778216-2778235 Msa0485220:CDS 40.0%
CAGCTACTAAGACCTTGAAT+AGG + chr3_4:2778321-2778340 Msa0485220:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG + chr3_4:2778323-2778342 Msa0485220:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG - chr3_4:2778386-2778405 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG - chr3_4:2778410-2778429 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG - chr3_4:2778625-2778644 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG + chr3_4:2779164-2779183 Msa0485220:CDS 40.0%
TCACATCAAAACAGGCACTT+GGG + chr3_4:2779172-2779191 Msa0485220:CDS 40.0%
AAGCCCATCATACCATCATA+CGG - chr3_4:2779433-2779452 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG - chr3_4:2779739-2779758 None:intergenic 40.0%
!! CTCAAAAAGGGTGCTAATGA+AGG + chr3_4:2778216-2778235 Msa0485220:CDS 40.0%
CAGCTACTAAGACCTTGAAT+AGG + chr3_4:2778321-2778340 Msa0485220:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG + chr3_4:2778323-2778342 Msa0485220:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG - chr3_4:2778386-2778405 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG - chr3_4:2778410-2778429 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG - chr3_4:2778625-2778644 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG + chr3_4:2779164-2779183 Msa0485220:CDS 40.0%
TCACATCAAAACAGGCACTT+GGG + chr3_4:2779172-2779191 Msa0485220:CDS 40.0%
AAGCCCATCATACCATCATA+CGG - chr3_4:2779433-2779452 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG - chr3_4:2779739-2779758 None:intergenic 40.0%
ATGAAAGCGGCTAGGGTTTA+TGG - chr3_4:2777953-2777972 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG - chr3_4:2777960-2777979 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG + chr3_4:2778119-2778138 Msa0485220:CDS 45.0%
TAACCCTAAGGCATACCCTT+TGG + chr3_4:2778131-2778150 Msa0485220:CDS 45.0%
TTAGCTGAGCATCAGCCAAA+GGG - chr3_4:2778149-2778168 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG + chr3_4:2778406-2778425 Msa0485220:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG - chr3_4:2778673-2778692 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG + chr3_4:2778670-2778689 Msa0485220:intron 45.0%
CGAGACCTAACAACATTCAG+AGG - chr3_4:2778874-2778893 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG + chr3_4:2779171-2779190 Msa0485220:CDS 45.0%
AGAACAAGCAATCACTGGTC+GGG - chr3_4:2779214-2779233 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG - chr3_4:2779215-2779234 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG - chr3_4:2779219-2779238 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG + chr3_4:2779263-2779282 Msa0485220:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG + chr3_4:2779457-2779476 Msa0485220:three_prime_UTR 45.0%
CACACAATAACAACCTTCGG+CGG - chr3_4:2779551-2779570 None:intergenic 45.0%
ATGAAAGCGGCTAGGGTTTA+TGG - chr3_4:2777953-2777972 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG - chr3_4:2777960-2777979 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG + chr3_4:2778119-2778138 Msa0485220:CDS 45.0%
TAACCCTAAGGCATACCCTT+TGG + chr3_4:2778131-2778150 Msa0485220:CDS 45.0%
TTAGCTGAGCATCAGCCAAA+GGG - chr3_4:2778149-2778168 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG + chr3_4:2778406-2778425 Msa0485220:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG - chr3_4:2778673-2778692 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG + chr3_4:2778670-2778689 Msa0485220:intron 45.0%
CGAGACCTAACAACATTCAG+AGG - chr3_4:2778874-2778893 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG + chr3_4:2779171-2779190 Msa0485220:CDS 45.0%
AGAACAAGCAATCACTGGTC+GGG - chr3_4:2779214-2779233 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG - chr3_4:2779215-2779234 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG - chr3_4:2779219-2779238 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG + chr3_4:2779263-2779282 Msa0485220:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG + chr3_4:2779457-2779476 Msa0485220:three_prime_UTR 45.0%
CACACAATAACAACCTTCGG+CGG - chr3_4:2779551-2779570 None:intergenic 45.0%
CGAAGAGAATGAAAGCGGCT+AGG - chr3_4:2777961-2777980 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG - chr3_4:2778137-2778156 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG - chr3_4:2778138-2778157 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG - chr3_4:2778150-2778169 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG - chr3_4:2778403-2778422 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG + chr3_4:2778400-2778419 Msa0485220:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG - chr3_4:2779486-2779505 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG + chr3_4:2779549-2779568 Msa0485220:three_prime_UTR 50.0%
CGAAGAGAATGAAAGCGGCT+AGG - chr3_4:2777961-2777980 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG - chr3_4:2778137-2778156 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG - chr3_4:2778138-2778157 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG - chr3_4:2778150-2778169 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG - chr3_4:2778403-2778422 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG + chr3_4:2778400-2778419 Msa0485220:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG - chr3_4:2779486-2779505 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG + chr3_4:2779549-2779568 Msa0485220:three_prime_UTR 50.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG + chr3_4:2779460-2779479 Msa0485220:three_prime_UTR 55.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG + chr3_4:2779460-2779479 Msa0485220:three_prime_UTR 55.0%
CAGGCACTTGGGCGTGCTTG+TGG + chr3_4:2779183-2779202 Msa0485220:CDS 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG + chr3_4:2779470-2779489 Msa0485220:three_prime_UTR 65.0%
CAGGCACTTGGGCGTGCTTG+TGG + chr3_4:2779183-2779202 Msa0485220:CDS 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG + chr3_4:2779470-2779489 Msa0485220:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 2777891 2779792 2777891 ID=Msa0485220;Name=Msa0485220
chr3_4 mRNA 2777891 2779792 2777891 ID=Msa0485220-mRNA-1;Parent=Msa0485220;Name=Msa0485220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108|1|1|1|0.75|0.8|5|375|128
chr3_4 exon 2777891 2778001 2777891 ID=Msa0485220-mRNA-1:exon:930;Parent=Msa0485220-mRNA-1
chr3_4 exon 2778117 2778237 2778117 ID=Msa0485220-mRNA-1:exon:931;Parent=Msa0485220-mRNA-1
chr3_4 exon 2778324 2778427 2778324 ID=Msa0485220-mRNA-1:exon:932;Parent=Msa0485220-mRNA-1
chr3_4 exon 2779153 2779284 2779153 ID=Msa0485220-mRNA-1:exon:933;Parent=Msa0485220-mRNA-1
chr3_4 exon 2779391 2779792 2779391 ID=Msa0485220-mRNA-1:exon:934;Parent=Msa0485220-mRNA-1
chr3_4 five_prime_UTR 2777891 2777998 2777891 ID=Msa0485220-mRNA-1:five_prime_utr;Parent=Msa0485220-mRNA-1
chr3_4 CDS 2777999 2778001 2777999 ID=Msa0485220-mRNA-1:cds;Parent=Msa0485220-mRNA-1
chr3_4 CDS 2778117 2778237 2778117 ID=Msa0485220-mRNA-1:cds;Parent=Msa0485220-mRNA-1
chr3_4 CDS 2778324 2778427 2778324 ID=Msa0485220-mRNA-1:cds;Parent=Msa0485220-mRNA-1
chr3_4 CDS 2779153 2779284 2779153 ID=Msa0485220-mRNA-1:cds;Parent=Msa0485220-mRNA-1
chr3_4 CDS 2779391 2779417 2779391 ID=Msa0485220-mRNA-1:cds;Parent=Msa0485220-mRNA-1
chr3_4 three_prime_UTR 2779418 2779792 2779418 ID=Msa0485220-mRNA-1:three_prime_utr;Parent=Msa0485220-mRNA-1
Gene Sequence

>Msa0485220

ATGACTGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGAATGTTCCCTATGTGTTCGTCACATCAAAACAGGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGATGCTATTGAGAAGCTGCTAATCTGA
Protein sequence

>Msa0485220

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI*