AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1213850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213850 MtrunA17_Chr4g0063571 100.000 128 0 0 1 128 1 128 1.30e-91 260
Msa1213850 MtrunA17_Chr2g0307081 97.656 128 3 0 1 128 1 128 3.04e-89 254
Msa1213850 MtrunA17_Chr4g0061981 34.483 116 75 1 4 118 17 132 1.14e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213850 AT4G22380.1 93.750 128 8 0 1 128 1 128 1.17e-76 223
Msa1213850 AT5G20160.1 92.969 128 9 0 1 128 1 128 4.97e-76 221
Msa1213850 AT4G12600.1 89.844 128 13 0 1 128 1 128 1.31e-72 213
Msa1213850 AT5G20160.2 74.375 160 9 1 1 128 1 160 3.95e-69 205
Msa1213850 AT4G12600.2 55.738 183 26 2 1 128 1 183 2.20e-49 156
Msa1213850 AT5G20160.3 65.741 108 5 1 1 76 1 108 1.15e-31 108
Msa1213850 AT5G08180.2 35.938 128 73 1 8 126 24 151 3.79e-21 83.6
Msa1213850 AT5G08180.1 35.938 128 73 1 8 126 24 151 3.79e-21 83.6

Find 50 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAGCGGCTAGGGTTTA+TGG 0.198145 8_1:-74054249 None:intergenic
TAACTATAAGCAACTCAAAA+AGG 0.236397 8_1:+74054502 Msa1213850:CDS
GAAGACTTTATATATATAAT+TGG 0.242176 8_1:-74054192 None:intergenic
TATTTCTGAATTTGTTGTTA+TGG 0.302213 8_1:+74054645 Msa1213850:CDS
TTGTTGAACAAGATCCATTA+TGG 0.342840 8_1:-74054474 None:intergenic
TCCGTTACTTGCTGAGGATA+AGG 0.383122 8_1:+74054705 Msa1213850:CDS
AGCTACTAAGACCTTGAATA+GGG 0.384258 8_1:+74054621 Msa1213850:intron
TCAGCCAAAGGGTATGCCTT+AGG 0.396497 8_1:-74054434 None:intergenic
AGTTGTGCTGAAAGCGTCTT+CGG 0.405427 8_1:+74055759 Msa1213850:three_prime_UTR
GCGTCTTCGGAGGCCTTGAC+TGG 0.409377 8_1:+74055772 Msa1213850:three_prime_UTR
CAGCTACTAAGACCTTGAAT+AGG 0.416543 8_1:+74054620 Msa1213850:intron
TTCAGAAATACCCCTATTCA+AGG 0.432400 8_1:-74054632 None:intergenic
GTCTTCGTTTCTTATAATAA+CGG 0.434891 8_1:+74054209 Msa1213850:five_prime_UTR
AACGGTACCATTGTTAATTG+AGG 0.440521 8_1:-74054286 None:intergenic
TAACCCTAAGGCATACCCTT+TGG 0.467615 8_1:+74054430 Msa1213850:CDS
TACCACACAATAACAACCTT+CGG 0.470397 8_1:-74055853 None:intergenic
TCAGCTAACAATCACCATAA+TGG 0.473714 8_1:+74054460 Msa1213850:CDS
CAGCCAAAGGGTATGCCTTA+GGG 0.474338 8_1:-74054433 None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG 0.475355 8_1:-74054699 None:intergenic
TGTGTTCGTCACATCAAAAC+AGG 0.479560 8_1:+74055466 Msa1213850:CDS
CCATCTTCCGTTACTTGCTG+AGG 0.512018 8_1:+74054699 Msa1213850:CDS
CGCCGAAGGTTGTTATTGTG+TGG 0.518320 8_1:+74055851 Msa1213850:three_prime_UTR
GTCACATCAAAACAGGCACT+TGG 0.523031 8_1:+74055473 Msa1213850:CDS
TTATCCGTATGATGGTATGA+TGG 0.527837 8_1:+74055728 Msa1213850:three_prime_UTR
AAACTTCATTATCCGTATGA+TGG 0.529098 8_1:+74055720 Msa1213850:three_prime_UTR
AGAACAAGCAATCACTGGTC+GGG 0.539786 8_1:-74055513 None:intergenic
GTAACACTCATGCATCATAC+AGG 0.540573 8_1:-74056037 None:intergenic
TATCCGTATGATGGTATGAT+GGG 0.540926 8_1:+74055729 Msa1213850:three_prime_UTR
GAAGAGAATGAAAGCGGCTA+GGG 0.556939 8_1:-74054256 None:intergenic
GTCACAGAACAAGCAATCAC+TGG 0.562045 8_1:-74055518 None:intergenic
AAACTGAATTTACTAGTCAA+AGG 0.565236 8_1:-74055939 None:intergenic
CGAAGAGAATGAAAGCGGCT+AGG 0.565826 8_1:-74054257 None:intergenic
TTTGATGTGACGAACACATA+GGG 0.566367 8_1:-74055461 None:intergenic
CAGGCACTTGGGCGTGCTTG+TGG 0.575874 8_1:+74055485 Msa1213850:CDS
CACAACAAGTCGTCCAGTCA+AGG 0.578850 8_1:-74055785 None:intergenic
TTAGCTGAGCATCAGCCAAA+GGG 0.590874 8_1:-74054445 None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG 0.593594 8_1:-74054706 None:intergenic
AAGCCCATCATACCATCATA+CGG 0.597239 8_1:-74055732 None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG 0.598758 8_1:-74054446 None:intergenic
GTCTCAGATTCAGCAACTCA+AGG 0.599622 8_1:+74055565 Msa1213850:CDS
GTTCAATCCTCAATTAACAA+TGG 0.604789 8_1:+74054279 Msa1213850:exon
TGTTCTGTGACAACTAATGA+AGG 0.613805 8_1:+74055530 Msa1213850:CDS
CAGAACAAGCAATCACTGGT+CGG 0.618383 8_1:-74055514 None:intergenic
TGGAGAAGCAGTTAACCCTA+AGG 0.621032 8_1:+74054418 Msa1213850:CDS
TCACATCAAAACAGGCACTT+GGG 0.623134 8_1:+74055474 Msa1213850:CDS
TGTGCTGAAAGCGTCTTCGG+AGG 0.641398 8_1:+74055762 Msa1213850:three_prime_UTR
AGATGGAGAAGAATCTCAAG+AGG 0.644746 8_1:-74054682 None:intergenic
GCTACTAAGACCTTGAATAG+GGG 0.659558 8_1:+74054622 Msa1213850:intron
TTGAACGAAGAGAATGAAAG+CGG 0.665410 8_1:-74054262 None:intergenic
CACACAATAACAACCTTCGG+CGG 0.714102 8_1:-74055850 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGAATAAGAAATTA+GGG - chr8_1:74054544-74054563 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG + chr8_1:74055012-74055031 Msa1213850:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG - chr8_1:74055366-74055385 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG + chr8_1:74055370-74055389 Msa1213850:intron 10.0%
!! AAAAAATGAATAAGAAATTA+GGG - chr8_1:74054544-74054563 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG + chr8_1:74055012-74055031 Msa1213850:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG - chr8_1:74055366-74055385 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG + chr8_1:74055370-74055389 Msa1213850:intron 10.0%
!! GAAGACTTTATATATATAAT+TGG - chr8_1:74054195-74054214 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG - chr8_1:74054307-74054326 None:intergenic 15.0%
!! CAAAAAATGAATAAGAAATT+AGG - chr8_1:74054545-74054564 None:intergenic 15.0%
!! AATTATCAATCAATAATGAA+AGG - chr8_1:74054585-74054604 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG - chr8_1:74055832-74055851 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG - chr8_1:74055907-74055926 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG + chr8_1:74056068-74056087 Msa1213850:three_prime_UTR 15.0%
!! CTTTATATATATAATTGGTT+TGG - chr8_1:74054190-74054209 None:intergenic 15.0%
!! GAAGACTTTATATATATAAT+TGG - chr8_1:74054195-74054214 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG - chr8_1:74054307-74054326 None:intergenic 15.0%
!! CAAAAAATGAATAAGAAATT+AGG - chr8_1:74054545-74054564 None:intergenic 15.0%
!! AATTATCAATCAATAATGAA+AGG - chr8_1:74054585-74054604 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG - chr8_1:74055832-74055851 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG - chr8_1:74055907-74055926 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG + chr8_1:74056068-74056087 Msa1213850:three_prime_UTR 15.0%
!! CAAATTAAGAGTTAAACATA+GGG - chr8_1:74054334-74054353 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG - chr8_1:74054335-74054354 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG + chr8_1:74054645-74054664 Msa1213850:CDS 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG + chr8_1:74054829-74054848 Msa1213850:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG - chr8_1:74055001-74055020 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG + chr8_1:74055659-74055678 Msa1213850:intron 20.0%
!! CAAATTAAGAGTTAAACATA+GGG - chr8_1:74054334-74054353 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG - chr8_1:74054335-74054354 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG + chr8_1:74054645-74054664 Msa1213850:CDS 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG + chr8_1:74054829-74054848 Msa1213850:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG - chr8_1:74055001-74055020 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG + chr8_1:74055659-74055678 Msa1213850:intron 20.0%
! GTCTTCGTTTCTTATAATAA+CGG + chr8_1:74054209-74054228 Msa1213850:five_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG + chr8_1:74054502-74054521 Msa1213850:CDS 25.0%
! AACTATAAGCAACTCAAAAA+GGG + chr8_1:74054503-74054522 Msa1213850:CDS 25.0%
! AGTAATATTACTTGAAGACA+TGG - chr8_1:74055244-74055263 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG - chr8_1:74055357-74055376 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG + chr8_1:74055663-74055682 Msa1213850:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG + chr8_1:74055895-74055914 Msa1213850:three_prime_UTR 25.0%
! AAACTGAATTTACTAGTCAA+AGG - chr8_1:74055942-74055961 None:intergenic 25.0%
! GTCTTCGTTTCTTATAATAA+CGG + chr8_1:74054209-74054228 Msa1213850:five_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG + chr8_1:74054502-74054521 Msa1213850:CDS 25.0%
! AACTATAAGCAACTCAAAAA+GGG + chr8_1:74054503-74054522 Msa1213850:CDS 25.0%
! AGTAATATTACTTGAAGACA+TGG - chr8_1:74055244-74055263 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG - chr8_1:74055357-74055376 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG + chr8_1:74055663-74055682 Msa1213850:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG + chr8_1:74055895-74055914 Msa1213850:three_prime_UTR 25.0%
! AAACTGAATTTACTAGTCAA+AGG - chr8_1:74055942-74055961 None:intergenic 25.0%
GTTCAATCCTCAATTAACAA+TGG + chr8_1:74054279-74054298 Msa1213850:exon 30.0%
ATGATGAAAAATTTGCAGAC+TGG + chr8_1:74054398-74054417 Msa1213850:intron 30.0%
TTGTTGAACAAGATCCATTA+TGG - chr8_1:74054477-74054496 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG - chr8_1:74054929-74054948 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG + chr8_1:74054934-74054953 Msa1213850:intron 30.0%
TTTGTATCCATACAGATGTT+TGG + chr8_1:74054965-74054984 Msa1213850:intron 30.0%
TTGTATCCATACAGATGTTT+GGG + chr8_1:74054966-74054985 Msa1213850:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG + chr8_1:74055061-74055080 Msa1213850:intron 30.0%
AAACTTCATTATCCGTATGA+TGG + chr8_1:74055720-74055739 Msa1213850:three_prime_UTR 30.0%
GTTCAATCCTCAATTAACAA+TGG + chr8_1:74054279-74054298 Msa1213850:exon 30.0%
ATGATGAAAAATTTGCAGAC+TGG + chr8_1:74054398-74054417 Msa1213850:intron 30.0%
TTGTTGAACAAGATCCATTA+TGG - chr8_1:74054477-74054496 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG - chr8_1:74054929-74054948 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG + chr8_1:74054934-74054953 Msa1213850:intron 30.0%
TTTGTATCCATACAGATGTT+TGG + chr8_1:74054965-74054984 Msa1213850:intron 30.0%
TTGTATCCATACAGATGTTT+GGG + chr8_1:74054966-74054985 Msa1213850:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG + chr8_1:74055061-74055080 Msa1213850:intron 30.0%
AAACTTCATTATCCGTATGA+TGG + chr8_1:74055720-74055739 Msa1213850:three_prime_UTR 30.0%
TTGAACGAAGAGAATGAAAG+CGG - chr8_1:74054265-74054284 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG - chr8_1:74054289-74054308 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG + chr8_1:74054460-74054479 Msa1213850:CDS 35.0%
AGCTACTAAGACCTTGAATA+GGG + chr8_1:74054621-74054640 Msa1213850:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG - chr8_1:74054635-74054654 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG + chr8_1:74054714-74054733 Msa1213850:intron 35.0%
GCTGAGGATAAGGTATATTA+GGG + chr8_1:74054715-74054734 Msa1213850:intron 35.0%
TGCTATGCATAACAAATAGC+GGG - chr8_1:74054928-74054947 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG + chr8_1:74055054-74055073 Msa1213850:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG + chr8_1:74055055-74055074 Msa1213850:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG - chr8_1:74055137-74055156 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG + chr8_1:74055135-74055154 Msa1213850:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG + chr8_1:74055168-74055187 Msa1213850:intron 35.0%
CATGGTTTATCACACTTGTT+TGG - chr8_1:74055226-74055245 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG - chr8_1:74055464-74055483 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG - chr8_1:74055465-74055484 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG + chr8_1:74055530-74055549 Msa1213850:CDS 35.0%
! TGAATATTTGGTTGGTATGC+AGG + chr8_1:74055671-74055690 Msa1213850:intron 35.0%
TTATCCGTATGATGGTATGA+TGG + chr8_1:74055728-74055747 Msa1213850:three_prime_UTR 35.0%
TATCCGTATGATGGTATGAT+GGG + chr8_1:74055729-74055748 Msa1213850:three_prime_UTR 35.0%
! AATTTTCTTTTTACCGCCGA+AGG + chr8_1:74055837-74055856 Msa1213850:three_prime_UTR 35.0%
TACCACACAATAACAACCTT+CGG - chr8_1:74055856-74055875 None:intergenic 35.0%
TTGAACGAAGAGAATGAAAG+CGG - chr8_1:74054265-74054284 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG - chr8_1:74054289-74054308 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG + chr8_1:74054460-74054479 Msa1213850:CDS 35.0%
AGCTACTAAGACCTTGAATA+GGG + chr8_1:74054621-74054640 Msa1213850:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG - chr8_1:74054635-74054654 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG + chr8_1:74054714-74054733 Msa1213850:intron 35.0%
GCTGAGGATAAGGTATATTA+GGG + chr8_1:74054715-74054734 Msa1213850:intron 35.0%
TGCTATGCATAACAAATAGC+GGG - chr8_1:74054928-74054947 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG + chr8_1:74055054-74055073 Msa1213850:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG + chr8_1:74055055-74055074 Msa1213850:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG - chr8_1:74055137-74055156 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG + chr8_1:74055135-74055154 Msa1213850:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG + chr8_1:74055168-74055187 Msa1213850:intron 35.0%
CATGGTTTATCACACTTGTT+TGG - chr8_1:74055226-74055245 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG - chr8_1:74055464-74055483 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG - chr8_1:74055465-74055484 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG + chr8_1:74055530-74055549 Msa1213850:CDS 35.0%
! TGAATATTTGGTTGGTATGC+AGG + chr8_1:74055671-74055690 Msa1213850:intron 35.0%
TTATCCGTATGATGGTATGA+TGG + chr8_1:74055728-74055747 Msa1213850:three_prime_UTR 35.0%
TATCCGTATGATGGTATGAT+GGG + chr8_1:74055729-74055748 Msa1213850:three_prime_UTR 35.0%
! AATTTTCTTTTTACCGCCGA+AGG + chr8_1:74055837-74055856 Msa1213850:three_prime_UTR 35.0%
TACCACACAATAACAACCTT+CGG - chr8_1:74055856-74055875 None:intergenic 35.0%
!! CTCAAAAAGGGTGCTAATGA+AGG + chr8_1:74054515-74054534 Msa1213850:CDS 40.0%
CAGCTACTAAGACCTTGAAT+AGG + chr8_1:74054620-74054639 Msa1213850:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG + chr8_1:74054622-74054641 Msa1213850:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG - chr8_1:74054685-74054704 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG - chr8_1:74054709-74054728 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG - chr8_1:74054927-74054946 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG + chr8_1:74055466-74055485 Msa1213850:CDS 40.0%
TCACATCAAAACAGGCACTT+GGG + chr8_1:74055474-74055493 Msa1213850:CDS 40.0%
AAGCCCATCATACCATCATA+CGG - chr8_1:74055735-74055754 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG - chr8_1:74056040-74056059 None:intergenic 40.0%
!! CTCAAAAAGGGTGCTAATGA+AGG + chr8_1:74054515-74054534 Msa1213850:CDS 40.0%
CAGCTACTAAGACCTTGAAT+AGG + chr8_1:74054620-74054639 Msa1213850:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG + chr8_1:74054622-74054641 Msa1213850:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG - chr8_1:74054685-74054704 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG - chr8_1:74054709-74054728 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG - chr8_1:74054927-74054946 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG + chr8_1:74055466-74055485 Msa1213850:CDS 40.0%
TCACATCAAAACAGGCACTT+GGG + chr8_1:74055474-74055493 Msa1213850:CDS 40.0%
AAGCCCATCATACCATCATA+CGG - chr8_1:74055735-74055754 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG - chr8_1:74056040-74056059 None:intergenic 40.0%
ATGAAAGCGGCTAGGGTTTA+TGG - chr8_1:74054252-74054271 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG - chr8_1:74054259-74054278 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG + chr8_1:74054418-74054437 Msa1213850:CDS 45.0%
TAACCCTAAGGCATACCCTT+TGG + chr8_1:74054430-74054449 Msa1213850:CDS 45.0%
TTAGCTGAGCATCAGCCAAA+GGG - chr8_1:74054448-74054467 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG + chr8_1:74054705-74054724 Msa1213850:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG - chr8_1:74054975-74054994 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG + chr8_1:74054972-74054991 Msa1213850:intron 45.0%
CGAGACCTAACAACATTCAG+AGG - chr8_1:74055176-74055195 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG + chr8_1:74055473-74055492 Msa1213850:CDS 45.0%
AGAACAAGCAATCACTGGTC+GGG - chr8_1:74055516-74055535 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG - chr8_1:74055517-74055536 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG - chr8_1:74055521-74055540 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG + chr8_1:74055565-74055584 Msa1213850:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG + chr8_1:74055759-74055778 Msa1213850:three_prime_UTR 45.0%
CACACAATAACAACCTTCGG+CGG - chr8_1:74055853-74055872 None:intergenic 45.0%
ATGAAAGCGGCTAGGGTTTA+TGG - chr8_1:74054252-74054271 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG - chr8_1:74054259-74054278 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG + chr8_1:74054418-74054437 Msa1213850:CDS 45.0%
TAACCCTAAGGCATACCCTT+TGG + chr8_1:74054430-74054449 Msa1213850:CDS 45.0%
TTAGCTGAGCATCAGCCAAA+GGG - chr8_1:74054448-74054467 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG + chr8_1:74054705-74054724 Msa1213850:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG - chr8_1:74054975-74054994 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG + chr8_1:74054972-74054991 Msa1213850:intron 45.0%
CGAGACCTAACAACATTCAG+AGG - chr8_1:74055176-74055195 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG + chr8_1:74055473-74055492 Msa1213850:CDS 45.0%
AGAACAAGCAATCACTGGTC+GGG - chr8_1:74055516-74055535 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG - chr8_1:74055517-74055536 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG - chr8_1:74055521-74055540 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG + chr8_1:74055565-74055584 Msa1213850:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG + chr8_1:74055759-74055778 Msa1213850:three_prime_UTR 45.0%
CACACAATAACAACCTTCGG+CGG - chr8_1:74055853-74055872 None:intergenic 45.0%
CGAAGAGAATGAAAGCGGCT+AGG - chr8_1:74054260-74054279 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG - chr8_1:74054436-74054455 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG - chr8_1:74054437-74054456 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG - chr8_1:74054449-74054468 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG - chr8_1:74054702-74054721 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG + chr8_1:74054699-74054718 Msa1213850:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG - chr8_1:74055788-74055807 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG + chr8_1:74055851-74055870 Msa1213850:three_prime_UTR 50.0%
CGAAGAGAATGAAAGCGGCT+AGG - chr8_1:74054260-74054279 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG - chr8_1:74054436-74054455 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG - chr8_1:74054437-74054456 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG - chr8_1:74054449-74054468 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG - chr8_1:74054702-74054721 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG + chr8_1:74054699-74054718 Msa1213850:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG - chr8_1:74055788-74055807 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG + chr8_1:74055851-74055870 Msa1213850:three_prime_UTR 50.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG + chr8_1:74055762-74055781 Msa1213850:three_prime_UTR 55.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG + chr8_1:74055762-74055781 Msa1213850:three_prime_UTR 55.0%
CAGGCACTTGGGCGTGCTTG+TGG + chr8_1:74055485-74055504 Msa1213850:CDS 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG + chr8_1:74055772-74055791 Msa1213850:three_prime_UTR 65.0%
CAGGCACTTGGGCGTGCTTG+TGG + chr8_1:74055485-74055504 Msa1213850:CDS 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG + chr8_1:74055772-74055791 Msa1213850:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 74054190 74056093 74054190 ID=Msa1213850;Name=Msa1213850
chr8_1 mRNA 74054190 74056093 74054190 ID=Msa1213850-mRNA-1;Parent=Msa1213850;Name=Msa1213850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108|1|1|1|0.75|0.8|5|374|128
chr8_1 exon 74054190 74054300 74054190 ID=Msa1213850-mRNA-1:exon:25309;Parent=Msa1213850-mRNA-1
chr8_1 exon 74054416 74054536 74054416 ID=Msa1213850-mRNA-1:exon:25310;Parent=Msa1213850-mRNA-1
chr8_1 exon 74054623 74054726 74054623 ID=Msa1213850-mRNA-1:exon:25311;Parent=Msa1213850-mRNA-1
chr8_1 exon 74055455 74055586 74055455 ID=Msa1213850-mRNA-1:exon:25312;Parent=Msa1213850-mRNA-1
chr8_1 exon 74055693 74056093 74055693 ID=Msa1213850-mRNA-1:exon:25313;Parent=Msa1213850-mRNA-1
chr8_1 five_prime_UTR 74054190 74054297 74054190 ID=Msa1213850-mRNA-1:five_prime_utr;Parent=Msa1213850-mRNA-1
chr8_1 CDS 74054298 74054300 74054298 ID=Msa1213850-mRNA-1:cds;Parent=Msa1213850-mRNA-1
chr8_1 CDS 74054416 74054536 74054416 ID=Msa1213850-mRNA-1:cds;Parent=Msa1213850-mRNA-1
chr8_1 CDS 74054623 74054726 74054623 ID=Msa1213850-mRNA-1:cds;Parent=Msa1213850-mRNA-1
chr8_1 CDS 74055455 74055586 74055455 ID=Msa1213850-mRNA-1:cds;Parent=Msa1213850-mRNA-1
chr8_1 CDS 74055693 74055719 74055693 ID=Msa1213850-mRNA-1:cds;Parent=Msa1213850-mRNA-1
chr8_1 three_prime_UTR 74055720 74056093 74055720 ID=Msa1213850-mRNA-1:three_prime_utr;Parent=Msa1213850-mRNA-1
Gene Sequence

>Msa1213850

ATGACTGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGAATGTTCCCTATGTGTTCGTCACATCAAAACAGGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGATGCTATTGAGAAGCTGCTAATCTGA
Protein sequence

>Msa1213850

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI*