AlfalfaGEDB | Msa1352150

M. sativa cultivar zhongmu-4 / Msa1352150

KEGG: K14165
GO: GO:0002791 GO:0002792 GO:0003674 GO:0003824 GO:0004439 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006629 GO:0006644 GO:0006650 GO:0006655 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0008962 GO:0009058 GO:0009987 GO:0010646 GO:0010648 GO:0010817 GO:0016020 GO:0016021 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0019866 GO:0023051 GO:0023057 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031975 GO:0032048 GO:0032049 GO:0032592 GO:0032879 GO:0032880 GO:0034593 GO:0034595 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045017 GO:0046471 GO:0046474 GO:0046486 GO:0046676 GO:0046883 GO:0046888 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0050796 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051223 GO:0051224 GO:0052866 GO:0060341 GO:0061178 GO:0061179 GO:0065007 GO:0065008 GO:0070201 GO:0071704 GO:0090087 GO:0090276 GO:0090278 GO:0090407 GO:0098573 GO:0106019 GO:1901576 GO:1903530 GO:1903531 GO:1904950
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352150	XP_003630688.1	99.145	117	1	0	1	117	1	117	5.13e-78	242

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352150	sp\|Q6NKR2\|TPMT2_ARATH	78.889	90	19	0	28	117	34	123	1.29e-48	157

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352150	G7L7D1	99.145	117	1	0	1	117	1	117	2.45e-78	242

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003220	Msa1352150	0.804535	2.062071e-49	-8.615850e-47
Msa0031180	Msa1352150	0.801734	7.830435e-49	-8.615850e-47
Msa0054530	Msa1352150	0.808558	2.920035e-50	-8.615850e-47
Msa0064920	Msa1352150	0.846814	1.715099e-59	-8.615850e-47
Msa0074750	Msa1352150	0.806836	6.779380e-50	-8.615850e-47
Msa0084340	Msa1352150	0.802434	5.620677e-49	-8.615850e-47
Msa0089980	Msa1352150	0.818213	2.204702e-52	-8.615850e-47
Msa0090010	Msa1352150	0.818452	1.946074e-52	-8.615850e-47
Msa0091780	Msa1352150	0.838247	3.221529e-57	-8.615850e-47
Msa1352150	Msa1372290	0.804864	1.760687e-49	-8.615850e-47
Msa1352150	Msa1393820	0.811541	6.648794e-51	-8.615850e-47
Msa1352150	Msa1426910	0.823471	1.360901e-53	-8.615850e-47
Msa1352150	Msa1435260	0.836011	1.201512e-56	-8.615850e-47
Msa1352150	Msa1450720	0.802637	5.104856e-49	-8.615850e-47
Msa0756380	Msa1352150	0.801127	1.042683e-48	-8.615850e-47
Msa0789560	Msa1352150	0.820186	7.833683e-53	-8.615850e-47
Msa0834750	Msa1352150	0.829533	4.882436e-55	-8.615850e-47
Msa0171870	Msa1352150	0.802801	4.722722e-49	-8.615850e-47
Msa0198300	Msa1352150	0.811988	5.314306e-51	-8.615850e-47
Msa0202730	Msa1352150	0.810644	1.040362e-50	-8.615850e-47
Msa0232340	Msa1352150	0.815102	1.098102e-51	-8.615850e-47
Msa0237200	Msa1352150	0.821376	4.172231e-53	-8.615850e-47
Msa0382370	Msa1352150	0.802146	6.444167e-49	-8.615850e-47
Msa0395980	Msa1352150	0.828107	1.080492e-54	-8.615850e-47
Msa0423840	Msa1352150	0.841909	3.569192e-58	-8.615850e-47
Msa0432160	Msa1352150	0.800498	1.401046e-48	-8.615850e-47
Msa0440510	Msa1352150	0.820715	5.924420e-53	-8.615850e-47
Msa0468250	Msa1352150	0.814479	1.508422e-51	-8.615850e-47
Msa0473980	Msa1352150	0.800159	1.642199e-48	-8.615850e-47
Msa0476680	Msa1352150	0.808536	2.951313e-50	-8.615850e-47
Msa0240650	Msa1352150	0.816777	4.643284e-52	-8.615850e-47
Msa0271790	Msa1352150	0.816471	5.437629e-52	-8.615850e-47
Msa0274970	Msa1352150	0.823221	1.556295e-53	-8.615850e-47
Msa0280150	Msa1352150	0.813862	2.064310e-51	-8.615850e-47
Msa0310430	Msa1352150	0.804443	2.155129e-49	-8.615850e-47
Msa0315680	Msa1352150	0.824249	8.940800e-54	-8.615850e-47
Msa0358400	Msa1352150	0.827909	1.205639e-54	-8.615850e-47
Msa0362890	Msa1352150	0.815953	7.097721e-52	-8.615850e-47
Msa1127430	Msa1352150	0.805707	1.171999e-49	-8.615850e-47
Msa0491000	Msa1352150	0.821840	3.260269e-53	-8.615850e-47
Msa0521010	Msa1352150	0.802991	4.314224e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352150	MtrunA17_Chr8g0389671	99.145	117	1	0	1	117	1	117	4.72e-82	242
Msa1352150	MtrunA17_Chr4g0055831	70.085	117	35	0	1	117	1	117	9.88e-53	168

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1352150	AT5G56610.1	78.889	90	19	28	117	34	123	1.31e-49	157
Msa1352150	AT2G35680.1	72.917	96	26	22	117	35	130	9.43e-48	156
Msa1352150	AT5G56610.2	76.923	39	9	28	66	34	72	1.47e-17	74.3

Find 53 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTACCTTATTACTAAATT+TGG	0.120439	tig0012581:+1319	None:intergenic
TTCAAGTGAGGAACATCTTT+AGG	0.249121	tig0012581:+356	None:intergenic
AGAGGAGAAAACAGGGAAAT+TGG	0.267287	tig0012581:+1398	None:intergenic
AATCGAAAATTAGCGATAAA+TGG	0.280192	tig0012581:+1358	None:intergenic
CGGTTCCAAATTTAGTAATA+AGG	0.281584	tig0012581:-1324	Msa1352150:intron
TCAAGTGAGGAACATCTTTA+GGG	0.305426	tig0012581:+357	None:intergenic
ATTTCGAGAGACAAATTGTT+AGG	0.308606	tig0012581:-1103	Msa1352150:CDS
TAGAGGATGCAGAGTGTAGT+AGG	0.335643	tig0012581:-1139	Msa1352150:CDS
ATTTATTTGTTTCAGGCTCA+TGG	0.339663	tig0012581:-29	Msa1352150:intron
GAATGAACCTTACGAAACTT+TGG	0.381107	tig0012581:-303	Msa1352150:CDS
GGCTCATGGGATCGATCATT+TGG	0.383349	tig0012581:-15	Msa1352150:CDS
TTGATGTGAGAGGAGAAAAC+AGG	0.406695	tig0012581:+1390	None:intergenic
ACAAAATTGAAACTGAGTTT+CGG	0.423331	tig0012581:-1001	Msa1352150:CDS
GATGAAGATCGAGGAATTAG+AGG	0.426977	tig0012581:-1156	Msa1352150:CDS
AGAGGATGCAGAGTGTAGTA+GGG	0.430320	tig0012581:-1138	Msa1352150:CDS
GTTCCGAGGGCATTCCTACT+CGG	0.437747	tig0012581:-1476	Msa1352150:five_prime_UTR
TTGTTAGGGTTGATGCTAAG+AGG	0.467061	tig0012581:-1088	Msa1352150:CDS
TTGAAGAACCTTGGTGTTGG+TGG	0.483829	tig0012581:-338	Msa1352150:CDS
TCCTCGATCTTCATCGCAAA+CGG	0.486060	tig0012581:+1164	None:intergenic
CGTTGGTTGGTGCAGGTGCA+CGG	0.492966	tig0012581:-1064	Msa1352150:CDS
TTTATTTGTTTCAGGCTCAT+GGG	0.493039	tig0012581:-28	Msa1352150:intron
GAGGAACATCTTTAGGGAAC+GGG	0.497949	tig0012581:+363	None:intergenic
TTCCGAGGGCATTCCTACTC+GGG	0.499228	tig0012581:-1475	Msa1352150:five_prime_UTR
AAGAGGGCGTTGGTTGGTGC+AGG	0.503871	tig0012581:-1071	Msa1352150:CDS
TTTCGAGAGACAAATTGTTA+GGG	0.504896	tig0012581:-1102	Msa1352150:CDS
GAAAGTGTTAGTAGAGTCGA+AGG	0.516859	tig0012581:+1434	None:intergenic
CACTTGAAGAACCTTGGTGT+TGG	0.517293	tig0012581:-341	Msa1352150:CDS
CGAAGGAGTAGAAACTAGAA+AGG	0.527195	tig0012581:+1451	None:intergenic
TGAAACTGAGTTTCGGTGGT+GGG	0.528954	tig0012581:-994	Msa1352150:CDS
AGTACATTATATAGAAGAGT+TGG	0.531536	tig0012581:+1029	None:intergenic
GTTCCTCACTTGAAGAACCT+TGG	0.547282	tig0012581:-347	Msa1352150:CDS
TTGAAACTGAGTTTCGGTGG+TGG	0.547833	tig0012581:-995	Msa1352150:CDS
TGATGTGAGAGGAGAAAACA+GGG	0.566835	tig0012581:+1391	None:intergenic
TGTTAGGGTTGATGCTAAGA+GGG	0.572220	tig0012581:-1087	Msa1352150:CDS
AACAGGGAAATTGGAAGAGA+CGG	0.574524	tig0012581:+1407	None:intergenic
GGTTGATGCTAAGAGGGCGT+TGG	0.576127	tig0012581:-1081	Msa1352150:CDS
GTAGTAAAACCTTGTTCCGA+GGG	0.582051	tig0012581:-1489	None:intergenic
GAGGATGCAGAGTGTAGTAG+GGG	0.592571	tig0012581:-1137	Msa1352150:CDS
GTAGGAATGCCCTCGGAACA+AGG	0.594851	tig0012581:+1480	None:intergenic
GATGCTAAGAGGGCGTTGGT+TGG	0.596262	tig0012581:-1077	Msa1352150:CDS
GATGGTACCAAAGTTTCGTA+AGG	0.596581	tig0012581:+296	None:intergenic
TGAGGAACATCTTTAGGGAA+CGG	0.601667	tig0012581:+362	None:intergenic
GTCCCGAGTAGGAATGCCCT+CGG	0.601684	tig0012581:+1473	None:intergenic
GGTAGTAAAACCTTGTTCCG+AGG	0.608262	tig0012581:-1490	None:intergenic
AAACTAGAAAGGTCCCGAGT+AGG	0.608876	tig0012581:+1462	None:intergenic
GTGGTGGGATCAAATTGATG+AGG	0.612151	tig0012581:-979	Msa1352150:intron
GAAAATTGATTTGATGTGAG+AGG	0.620012	tig0012581:+1380	None:intergenic
TCCGTTTGCGATGAAGATCG+AGG	0.626043	tig0012581:-1165	Msa1352150:exon
AAATTGAAACTGAGTTTCGG+TGG	0.642937	tig0012581:-998	Msa1352150:CDS
ACACCAAGGTTCTTCAAGTG+AGG	0.643756	tig0012581:+344	None:intergenic
GGAAATTGGAAGAGACGGTG+TGG	0.682880	tig0012581:+1412	None:intergenic
CTTACATGATACAACGACGA+TGG	0.702815	tig0012581:+278	None:intergenic
TAATTACACCACCAACACCA+AGG	0.708843	tig0012581:+330	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAATTATTATTTATTAGTT+AGG	-	tig0012581:995-1014	Msa1352150:CDS	10.0%
!!	GAATTATTATTTATTAGTTA+GGG	-	tig0012581:996-1015	Msa1352150:CDS	10.0%
!!	TGAATTATTATTTATTAGTT+AGG	-	tig0012581:995-1014	Msa1352150:CDS	10.0%
!!	GAATTATTATTTATTAGTTA+GGG	-	tig0012581:996-1015	Msa1352150:CDS	10.0%
!!	AAAAACCTAAAAATTCAAAA+GGG	+	tig0012581:223-242	None:intergenic	15.0%
!!	TAAAAACCTAAAAATTCAAA+AGG	+	tig0012581:224-243	None:intergenic	15.0%
!!!	TTATTTGATTTATTTGTTTC+AGG	-	tig0012581:1450-1469	Msa1352150:five_prime_UTR	15.0%
!!	AAAAACCTAAAAATTCAAAA+GGG	+	tig0012581:223-242	None:intergenic	15.0%
!!	TAAAAACCTAAAAATTCAAA+AGG	+	tig0012581:224-243	None:intergenic	15.0%
!!!	TTATTTGATTTATTTGTTTC+AGG	-	tig0012581:1450-1469	Msa1352150:five_prime_UTR	15.0%
!!	AGTTACCTTATTACTAAATT+TGG	+	tig0012581:170-189	None:intergenic	20.0%
!!	AAGATAATGAAATCAATGAA+AGG	+	tig0012581:199-218	None:intergenic	20.0%
!!!	AAATTGCAATTAGGAATTTT+TGG	-	tig0012581:1301-1320	Msa1352150:intron	20.0%
!!	AACAAATACAAACATAACAA+AGG	+	tig0012581:1406-1425	None:intergenic	20.0%
!!!	TAAAACTATTTCTGATATGT+TGG	-	tig0012581:1426-1445	Msa1352150:five_prime_UTR	20.0%
!!	AGTTACCTTATTACTAAATT+TGG	+	tig0012581:170-189	None:intergenic	20.0%
!!	AAGATAATGAAATCAATGAA+AGG	+	tig0012581:199-218	None:intergenic	20.0%
!!!	AAATTGCAATTAGGAATTTT+TGG	-	tig0012581:1301-1320	Msa1352150:intron	20.0%
!!	AACAAATACAAACATAACAA+AGG	+	tig0012581:1406-1425	None:intergenic	20.0%
!!!	TAAAACTATTTCTGATATGT+TGG	-	tig0012581:1426-1445	Msa1352150:five_prime_UTR	20.0%
!	AATCGAAAATTAGCGATAAA+TGG	+	tig0012581:131-150	None:intergenic	25.0%
!!	TTTTCGATTTTACGTCTAAT+CGG	-	tig0012581:142-161	Msa1352150:intron	25.0%
!!	TCTTTCCCTTTTGAATTTTT+AGG	-	tig0012581:215-234	Msa1352150:intron	25.0%
!	AGTACATTATATAGAAGAGT+TGG	+	tig0012581:460-479	None:intergenic	25.0%
!	ACAAAATTGAAACTGAGTTT+CGG	-	tig0012581:485-504	Msa1352150:intron	25.0%
!	ATTCTGAATTAGTGTATGTT+TGG	-	tig0012581:594-613	Msa1352150:intron	25.0%
!	TTCTGAATTAGTGTATGTTT+GGG	-	tig0012581:595-614	Msa1352150:intron	25.0%
!	TCAAGTATAATCAATTCTGT+AGG	-	tig0012581:762-781	Msa1352150:intron	25.0%
!	ATCAAAGAACATTCATAACA+TGG	+	tig0012581:1025-1044	None:intergenic	25.0%
!	TGTTATGAATGTTCTTTGAT+TGG	-	tig0012581:1025-1044	Msa1352150:CDS	25.0%
!!!	TTATTTCTAGTTTTTGTTGC+TGG	-	tig0012581:1093-1112	Msa1352150:CDS	25.0%
!!!	TATTTCTAGTTTTTGTTGCT+GGG	-	tig0012581:1094-1113	Msa1352150:CDS	25.0%
!	GATAGTCTAAAAAGCAAAAA+GGG	+	tig0012581:1240-1259	None:intergenic	25.0%
!	AGATAGTCTAAAAAGCAAAA+AGG	+	tig0012581:1241-1260	None:intergenic	25.0%
!	AAGCTAATTGTATGTTATGA+AGG	-	tig0012581:1277-1296	Msa1352150:intron	25.0%
!	TATGAAGGAAAATTGCAATT+AGG	-	tig0012581:1292-1311	Msa1352150:intron	25.0%
!!	TTGGAAAGAGTATATATAGT+TGG	-	tig0012581:1320-1339	Msa1352150:intron	25.0%
!!	TGGAAAGAGTATATATAGTT+GGG	-	tig0012581:1321-1340	Msa1352150:intron	25.0%
!	AATCGAAAATTAGCGATAAA+TGG	+	tig0012581:131-150	None:intergenic	25.0%
!!	TTTTCGATTTTACGTCTAAT+CGG	-	tig0012581:142-161	Msa1352150:intron	25.0%
!!	TCTTTCCCTTTTGAATTTTT+AGG	-	tig0012581:215-234	Msa1352150:intron	25.0%
!	AGTACATTATATAGAAGAGT+TGG	+	tig0012581:460-479	None:intergenic	25.0%
!	ACAAAATTGAAACTGAGTTT+CGG	-	tig0012581:485-504	Msa1352150:intron	25.0%
!	ATTCTGAATTAGTGTATGTT+TGG	-	tig0012581:594-613	Msa1352150:intron	25.0%
!	TTCTGAATTAGTGTATGTTT+GGG	-	tig0012581:595-614	Msa1352150:intron	25.0%
!	TCAAGTATAATCAATTCTGT+AGG	-	tig0012581:762-781	Msa1352150:intron	25.0%
!	ATCAAAGAACATTCATAACA+TGG	+	tig0012581:1025-1044	None:intergenic	25.0%
!	TGTTATGAATGTTCTTTGAT+TGG	-	tig0012581:1025-1044	Msa1352150:CDS	25.0%
!!!	TTATTTCTAGTTTTTGTTGC+TGG	-	tig0012581:1093-1112	Msa1352150:CDS	25.0%
!!!	TATTTCTAGTTTTTGTTGCT+GGG	-	tig0012581:1094-1113	Msa1352150:CDS	25.0%
!	GATAGTCTAAAAAGCAAAAA+GGG	+	tig0012581:1240-1259	None:intergenic	25.0%
!	AGATAGTCTAAAAAGCAAAA+AGG	+	tig0012581:1241-1260	None:intergenic	25.0%
!	AAGCTAATTGTATGTTATGA+AGG	-	tig0012581:1277-1296	Msa1352150:intron	25.0%
!	TATGAAGGAAAATTGCAATT+AGG	-	tig0012581:1292-1311	Msa1352150:intron	25.0%
!!	TTGGAAAGAGTATATATAGT+TGG	-	tig0012581:1320-1339	Msa1352150:intron	25.0%
!!	TGGAAAGAGTATATATAGTT+GGG	-	tig0012581:1321-1340	Msa1352150:intron	25.0%
!!	GAAAATTGATTTGATGTGAG+AGG	+	tig0012581:109-128	None:intergenic	30.0%
	CGGTTCCAAATTTAGTAATA+AGG	-	tig0012581:162-181	Msa1352150:intron	30.0%
	ATTATGTGAATCTGAATCGT+GGG	-	tig0012581:264-283	Msa1352150:intron	30.0%
	ATTTCGAGAGACAAATTGTT+AGG	-	tig0012581:383-402	Msa1352150:CDS	30.0%
	TTTCGAGAGACAAATTGTTA+GGG	-	tig0012581:384-403	Msa1352150:CDS	30.0%
!	TGAAGGTGTAGAATTGAATT+CGG	+	tig0012581:823-842	None:intergenic	30.0%
!	TACGCTAAAATTGATTCTGA+AGG	+	tig0012581:840-859	None:intergenic	30.0%
!!	GAATCAATTTTAGCGTAAGA+TGG	-	tig0012581:843-862	Msa1352150:intron	30.0%
	ATTTATTTGTTTCAGGCTCA+TGG	-	tig0012581:1457-1476	Msa1352150:five_prime_UTR	30.0%
	TTTATTTGTTTCAGGCTCAT+GGG	-	tig0012581:1458-1477	Msa1352150:five_prime_UTR	30.0%
!!	GAAAATTGATTTGATGTGAG+AGG	+	tig0012581:109-128	None:intergenic	30.0%
	CGGTTCCAAATTTAGTAATA+AGG	-	tig0012581:162-181	Msa1352150:intron	30.0%
	ATTATGTGAATCTGAATCGT+GGG	-	tig0012581:264-283	Msa1352150:intron	30.0%
	ATTTCGAGAGACAAATTGTT+AGG	-	tig0012581:383-402	Msa1352150:CDS	30.0%
	TTTCGAGAGACAAATTGTTA+GGG	-	tig0012581:384-403	Msa1352150:CDS	30.0%
!	TGAAGGTGTAGAATTGAATT+CGG	+	tig0012581:823-842	None:intergenic	30.0%
!	TACGCTAAAATTGATTCTGA+AGG	+	tig0012581:840-859	None:intergenic	30.0%
!!	GAATCAATTTTAGCGTAAGA+TGG	-	tig0012581:843-862	Msa1352150:intron	30.0%
	ATTTATTTGTTTCAGGCTCA+TGG	-	tig0012581:1457-1476	Msa1352150:five_prime_UTR	30.0%
	TTTATTTGTTTCAGGCTCAT+GGG	-	tig0012581:1458-1477	Msa1352150:five_prime_UTR	30.0%
	GATTATGTGAATCTGAATCG+TGG	-	tig0012581:263-282	Msa1352150:intron	35.0%
	AAATTGAAACTGAGTTTCGG+TGG	-	tig0012581:488-507	Msa1352150:intron	35.0%
!	TTAGTGTATGTTTGGGTTTG+CGG	-	tig0012581:602-621	Msa1352150:intron	35.0%
!!!	CTTCAACTCACTTTTTAGTC+AGG	+	tig0012581:657-676	None:intergenic	35.0%
!	CTGACTAAAAAGTGAGTTGA+AGG	-	tig0012581:655-674	Msa1352150:intron	35.0%
!!	GTTGAAGGTAAACTGATTTG+TGG	-	tig0012581:670-689	Msa1352150:intron	35.0%
	GAATTGAATTCGGACAAGTT+TGG	+	tig0012581:813-832	None:intergenic	35.0%
!	TGTTCTTTGATTGGTGTTTG+TGG	-	tig0012581:1034-1053	Msa1352150:CDS	35.0%
	TCAAGTGAGGAACATCTTTA+GGG	+	tig0012581:1132-1151	None:intergenic	35.0%
	TTCAAGTGAGGAACATCTTT+AGG	+	tig0012581:1133-1152	None:intergenic	35.0%
	GAATGAACCTTACGAAACTT+TGG	-	tig0012581:1183-1202	Msa1352150:five_prime_UTR	35.0%
	GATTATGTGAATCTGAATCG+TGG	-	tig0012581:263-282	Msa1352150:intron	35.0%
	AAATTGAAACTGAGTTTCGG+TGG	-	tig0012581:488-507	Msa1352150:intron	35.0%
!	TTAGTGTATGTTTGGGTTTG+CGG	-	tig0012581:602-621	Msa1352150:intron	35.0%
!!!	CTTCAACTCACTTTTTAGTC+AGG	+	tig0012581:657-676	None:intergenic	35.0%
!	CTGACTAAAAAGTGAGTTGA+AGG	-	tig0012581:655-674	Msa1352150:intron	35.0%
!!	GTTGAAGGTAAACTGATTTG+TGG	-	tig0012581:670-689	Msa1352150:intron	35.0%
	GAATTGAATTCGGACAAGTT+TGG	+	tig0012581:813-832	None:intergenic	35.0%
!	TGTTCTTTGATTGGTGTTTG+TGG	-	tig0012581:1034-1053	Msa1352150:CDS	35.0%
	TCAAGTGAGGAACATCTTTA+GGG	+	tig0012581:1132-1151	None:intergenic	35.0%
	TTCAAGTGAGGAACATCTTT+AGG	+	tig0012581:1133-1152	None:intergenic	35.0%
	GAATGAACCTTACGAAACTT+TGG	-	tig0012581:1183-1202	Msa1352150:five_prime_UTR	35.0%
	CGAAGGAGTAGAAACTAGAA+AGG	+	tig0012581:38-57	None:intergenic	40.0%
!!	GAAAGTGTTAGTAGAGTCGA+AGG	+	tig0012581:55-74	None:intergenic	40.0%
	AACAGGGAAATTGGAAGAGA+CGG	+	tig0012581:82-101	None:intergenic	40.0%
	AGAGGAGAAAACAGGGAAAT+TGG	+	tig0012581:91-110	None:intergenic	40.0%
	TGATGTGAGAGGAGAAAACA+GGG	+	tig0012581:98-117	None:intergenic	40.0%
	TTGATGTGAGAGGAGAAAAC+AGG	+	tig0012581:99-118	None:intergenic	40.0%
	GATGAAGATCGAGGAATTAG+AGG	-	tig0012581:330-349	Msa1352150:CDS	40.0%
!!	TTGTTAGGGTTGATGCTAAG+AGG	-	tig0012581:398-417	Msa1352150:intron	40.0%
!!	TGTTAGGGTTGATGCTAAGA+GGG	-	tig0012581:399-418	Msa1352150:intron	40.0%
!	TGAGGAACATCTTTAGGGAA+CGG	+	tig0012581:1127-1146	None:intergenic	40.0%
	TAATTACACCACCAACACCA+AGG	+	tig0012581:1159-1178	None:intergenic	40.0%
	GATGGTACCAAAGTTTCGTA+AGG	+	tig0012581:1193-1212	None:intergenic	40.0%
	CTTACATGATACAACGACGA+TGG	+	tig0012581:1211-1230	None:intergenic	40.0%
	TCGTCGTTGTATCATGTAAG+TGG	-	tig0012581:1211-1230	Msa1352150:intron	40.0%
	TGGGTTGCGTTCAATTAGTA+CGG	-	tig0012581:1340-1359	Msa1352150:five_prime_UTR	40.0%
	CGAAGGAGTAGAAACTAGAA+AGG	+	tig0012581:38-57	None:intergenic	40.0%
!!	GAAAGTGTTAGTAGAGTCGA+AGG	+	tig0012581:55-74	None:intergenic	40.0%
	AACAGGGAAATTGGAAGAGA+CGG	+	tig0012581:82-101	None:intergenic	40.0%
	AGAGGAGAAAACAGGGAAAT+TGG	+	tig0012581:91-110	None:intergenic	40.0%
	TGATGTGAGAGGAGAAAACA+GGG	+	tig0012581:98-117	None:intergenic	40.0%
	TTGATGTGAGAGGAGAAAAC+AGG	+	tig0012581:99-118	None:intergenic	40.0%
	GATGAAGATCGAGGAATTAG+AGG	-	tig0012581:330-349	Msa1352150:CDS	40.0%
!!	TTGTTAGGGTTGATGCTAAG+AGG	-	tig0012581:398-417	Msa1352150:intron	40.0%
!!	TGTTAGGGTTGATGCTAAGA+GGG	-	tig0012581:399-418	Msa1352150:intron	40.0%
!	TGAGGAACATCTTTAGGGAA+CGG	+	tig0012581:1127-1146	None:intergenic	40.0%
	TAATTACACCACCAACACCA+AGG	+	tig0012581:1159-1178	None:intergenic	40.0%
	GATGGTACCAAAGTTTCGTA+AGG	+	tig0012581:1193-1212	None:intergenic	40.0%
	CTTACATGATACAACGACGA+TGG	+	tig0012581:1211-1230	None:intergenic	40.0%
	TCGTCGTTGTATCATGTAAG+TGG	-	tig0012581:1211-1230	Msa1352150:intron	40.0%
	TGGGTTGCGTTCAATTAGTA+CGG	-	tig0012581:1340-1359	Msa1352150:five_prime_UTR	40.0%
	AAACTAGAAAGGTCCCGAGT+AGG	+	tig0012581:27-46	None:intergenic	45.0%
!	TGAATCGTGGGTGTGCTTTT+AGG	-	tig0012581:276-295	Msa1352150:intron	45.0%
!	GAATCGTGGGTGTGCTTTTA+GGG	-	tig0012581:277-296	Msa1352150:intron	45.0%
	TCCTCGATCTTCATCGCAAA+CGG	+	tig0012581:325-344	None:intergenic	45.0%
	TAGAGGATGCAGAGTGTAGT+AGG	-	tig0012581:347-366	Msa1352150:CDS	45.0%
	AGAGGATGCAGAGTGTAGTA+GGG	-	tig0012581:348-367	Msa1352150:CDS	45.0%
	TTGAAACTGAGTTTCGGTGG+TGG	-	tig0012581:491-510	Msa1352150:intron	45.0%
	TGAAACTGAGTTTCGGTGGT+GGG	-	tig0012581:492-511	Msa1352150:intron	45.0%
!	GTGGTGGGATCAAATTGATG+AGG	-	tig0012581:507-526	Msa1352150:intron	45.0%
!	GAGGAACATCTTTAGGGAAC+GGG	+	tig0012581:1126-1145	None:intergenic	45.0%
	GTTCCTCACTTGAAGAACCT+TGG	-	tig0012581:1139-1158	Msa1352150:CDS	45.0%
	ACACCAAGGTTCTTCAAGTG+AGG	+	tig0012581:1145-1164	None:intergenic	45.0%
!	CACTTGAAGAACCTTGGTGT+TGG	-	tig0012581:1145-1164	Msa1352150:CDS	45.0%
!!	TTGAAGAACCTTGGTGTTGG+TGG	-	tig0012581:1148-1167	Msa1352150:CDS	45.0%
	GGGTTGCGTTCAATTAGTAC+GGG	-	tig0012581:1341-1360	Msa1352150:five_prime_UTR	45.0%
	AAACTAGAAAGGTCCCGAGT+AGG	+	tig0012581:27-46	None:intergenic	45.0%
!	TGAATCGTGGGTGTGCTTTT+AGG	-	tig0012581:276-295	Msa1352150:intron	45.0%
!	GAATCGTGGGTGTGCTTTTA+GGG	-	tig0012581:277-296	Msa1352150:intron	45.0%
	TCCTCGATCTTCATCGCAAA+CGG	+	tig0012581:325-344	None:intergenic	45.0%
	TAGAGGATGCAGAGTGTAGT+AGG	-	tig0012581:347-366	Msa1352150:CDS	45.0%
	AGAGGATGCAGAGTGTAGTA+GGG	-	tig0012581:348-367	Msa1352150:CDS	45.0%
	TTGAAACTGAGTTTCGGTGG+TGG	-	tig0012581:491-510	Msa1352150:intron	45.0%
	TGAAACTGAGTTTCGGTGGT+GGG	-	tig0012581:492-511	Msa1352150:intron	45.0%
!	GTGGTGGGATCAAATTGATG+AGG	-	tig0012581:507-526	Msa1352150:intron	45.0%
!	GAGGAACATCTTTAGGGAAC+GGG	+	tig0012581:1126-1145	None:intergenic	45.0%
	GTTCCTCACTTGAAGAACCT+TGG	-	tig0012581:1139-1158	Msa1352150:CDS	45.0%
	ACACCAAGGTTCTTCAAGTG+AGG	+	tig0012581:1145-1164	None:intergenic	45.0%
!	CACTTGAAGAACCTTGGTGT+TGG	-	tig0012581:1145-1164	Msa1352150:CDS	45.0%
!!	TTGAAGAACCTTGGTGTTGG+TGG	-	tig0012581:1148-1167	Msa1352150:CDS	45.0%
	GGGTTGCGTTCAATTAGTAC+GGG	-	tig0012581:1341-1360	Msa1352150:five_prime_UTR	45.0%
	GGAAATTGGAAGAGACGGTG+TGG	+	tig0012581:77-96	None:intergenic	50.0%
	TCCGTTTGCGATGAAGATCG+AGG	-	tig0012581:321-340	Msa1352150:CDS	50.0%
	GAGGATGCAGAGTGTAGTAG+GGG	-	tig0012581:349-368	Msa1352150:CDS	50.0%
	GGCTCATGGGATCGATCATT+TGG	-	tig0012581:1471-1490	Msa1352150:five_prime_UTR	50.0%
	TAGGAATGCCCTCGGAACAA+GGG	+	tig0012581:8-27	None:intergenic	50.0%
	GGAAATTGGAAGAGACGGTG+TGG	+	tig0012581:77-96	None:intergenic	50.0%
	TCCGTTTGCGATGAAGATCG+AGG	-	tig0012581:321-340	Msa1352150:CDS	50.0%
	GAGGATGCAGAGTGTAGTAG+GGG	-	tig0012581:349-368	Msa1352150:CDS	50.0%
	GGCTCATGGGATCGATCATT+TGG	-	tig0012581:1471-1490	Msa1352150:five_prime_UTR	50.0%
!!	GTTCCGAGGGCATTCCTACT+CGG	-	tig0012581:10-29	Msa1352150:CDS	55.0%
!!	TTCCGAGGGCATTCCTACTC+GGG	-	tig0012581:11-30	Msa1352150:CDS	55.0%
!	GGTTGATGCTAAGAGGGCGT+TGG	-	tig0012581:405-424	Msa1352150:intron	55.0%
!!	GATGCTAAGAGGGCGTTGGT+TGG	-	tig0012581:409-428	Msa1352150:intron	55.0%
	AGGAATGCCCTCGGAACAAG+GGG	+	tig0012581:7-26	None:intergenic	55.0%
	GTAGGAATGCCCTCGGAACA+AGG	+	tig0012581:9-28	None:intergenic	55.0%
!!	GTTCCGAGGGCATTCCTACT+CGG	-	tig0012581:10-29	Msa1352150:CDS	55.0%
!!	TTCCGAGGGCATTCCTACTC+GGG	-	tig0012581:11-30	Msa1352150:CDS	55.0%
!	GGTTGATGCTAAGAGGGCGT+TGG	-	tig0012581:405-424	Msa1352150:intron	55.0%
!!	GATGCTAAGAGGGCGTTGGT+TGG	-	tig0012581:409-428	Msa1352150:intron	55.0%
	GTCCCGAGTAGGAATGCCCT+CGG	+	tig0012581:16-35	None:intergenic	60.0%
!!	AAGAGGGCGTTGGTTGGTGC+AGG	-	tig0012581:415-434	Msa1352150:intron	60.0%
!	CGTTGGTTGGTGCAGGTGCA+CGG	-	tig0012581:422-441	Msa1352150:intron	60.0%
	GTCCCGAGTAGGAATGCCCT+CGG	+	tig0012581:16-35	None:intergenic	60.0%
!!	AAGAGGGCGTTGGTTGGTGC+AGG	-	tig0012581:415-434	Msa1352150:intron	60.0%
!	CGTTGGTTGGTGCAGGTGCA+CGG	-	tig0012581:422-441	Msa1352150:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0012581	gene	7	1501	7	ID=Msa1352150;Name=Msa1352150
tig0012581	mRNA	7	1501	7	ID=Msa1352150-mRNA-1;Parent=Msa1352150;Name=Msa1352150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=210\|1\|0.75\|1\|0.66\|0.5\|4\|0\|117
tig0012581	exon	7	36	7	ID=Msa1352150-mRNA-1:exon:8051;Parent=Msa1352150-mRNA-1
tig0012581	exon	283	405	283	ID=Msa1352150-mRNA-1:exon:8050;Parent=Msa1352150-mRNA-1
tig0012581	exon	980	1210	980	ID=Msa1352150-mRNA-1:exon:8049;Parent=Msa1352150-mRNA-1
tig0012581	exon	1325	1501	1325	ID=Msa1352150-mRNA-1:exon:8048;Parent=Msa1352150-mRNA-1
tig0012581	five_prime_UTR	1325	1501	1325	ID=Msa1352150-mRNA-1:five_prime_utr;Parent=Msa1352150-mRNA-1
tig0012581	five_prime_UTR	1178	1210	1178	ID=Msa1352150-mRNA-1:five_prime_utr;Parent=Msa1352150-mRNA-1
tig0012581	CDS	980	1177	980	ID=Msa1352150-mRNA-1:cds;Parent=Msa1352150-mRNA-1
tig0012581	CDS	283	405	283	ID=Msa1352150-mRNA-1:cds;Parent=Msa1352150-mRNA-1
tig0012581	CDS	7	36	7	ID=Msa1352150-mRNA-1:cds;Parent=Msa1352150-mRNA-1

Gene Sequence	>Msa1352150 ATGAAGATCGAGGAATTAGAGGATGCAGAGTGTAGTAGGGGTGAAGAAGAGAATTTCGAGAGACAAATTGTTAGGGTTGATGCTAAGAGGGCGTTGGTTGGTGCAGGTGCACGGATCTTGTTTTATCCAACTCTTCTATATAATGTACTTCGTAACAAAATTGAAACTGAGTTTCGGTGGTGGGATCAAATTGATGAGTTTTTGTTGCTGGGCGCAGTCCCGTTCCCTAAAGATGTTCCTCACTTGAAGAACCTTGGTGTTGGTGGTGTAATTACTTTGAATGAACCTTACGAAACTTTGGTACCATCGTCGTTGTATCATGCTCATGGGATCGATCATTTGGTAATTCCC
Protein sequence	>Msa1352150 MKIEELEDAECSRGEEENFERQIVRVDAKRALVGAGARILFYPTLLYNVLRNKIETEFRWWDQIDEFLLLGAVPFPKDVPHLKNLGVGGVITLNEPYETLVPSSLYHAHGIDHLVIP