AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388550 MtrunA17_Chr8g0348681 90.343 321 31 0 1 321 9 329 0.0 573
Msa1388550 MtrunA17_Chr8g0348691 93.103 261 16 1 325 583 1 261 3.35e-174 493
Msa1388550 MtrunA17_Chr7g0248041 43.023 602 289 16 1 582 1 568 1.38e-141 423
Msa1388550 MtrunA17_Chr8g0387141 38.720 328 185 8 251 572 246 563 2.54e-61 213
Msa1388550 MtrunA17_Chr8g0387181 36.538 364 185 9 250 572 237 595 2.52e-60 211
Msa1388550 MtrunA17_Chr8g0387161 34.615 312 154 7 263 572 1 264 4.71e-49 172
Msa1388550 MtrunA17_Chr1g0186191 34.603 315 183 6 277 572 252 562 6.44e-49 179
Msa1388550 MtrunA17_Chr1g0186281 35.256 312 173 9 277 572 254 552 1.41e-48 178
Msa1388550 MtrunA17_Chr1g0186261 46.591 176 92 2 397 572 382 555 1.38e-45 169
Msa1388550 MtrunA17_Chr6g0479271 36.694 248 133 9 251 497 71 295 3.18e-31 124
Msa1388550 MtrunA17_Chr7g0241681 35.450 189 109 5 382 564 179 360 2.82e-28 116
Msa1388550 MtrunA17_Chr3g0086341 34.066 182 105 5 400 570 149 326 4.75e-22 99.8
Msa1388550 MtrunA17_Chr7g0241661 30.890 191 119 4 380 564 169 352 7.22e-20 91.7
Msa1388550 MtrunA17_Chr5g0420461 31.429 175 88 5 323 497 22 164 2.18e-16 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388550 AT2G46040.3 39.938 323 153 9 253 570 244 530 1.46e-64 221
Msa1388550 AT2G46040.1 39.938 323 153 9 253 570 244 530 1.46e-64 221
Msa1388550 AT2G46040.2 39.938 323 153 9 253 570 258 544 1.54e-64 222
Msa1388550 AT4G11400.2 34.694 343 184 8 263 581 225 551 6.24e-55 196
Msa1388550 AT4G11400.1 34.694 343 184 8 263 581 225 551 6.24e-55 196
Msa1388550 AT5G04110.1 38.415 164 87 5 400 552 361 521 4.56e-28 119
Msa1388550 AT5G04110.3 38.415 164 87 5 400 552 361 521 4.56e-28 119
Msa1388550 AT5G04110.2 38.415 164 87 5 400 552 362 522 4.59e-28 119
Msa1388550 AT5G04110.4 36.735 147 79 5 400 535 361 504 1.76e-20 95.5
Msa1388550 AT1G26580.3 32.124 193 110 6 396 570 65 254 6.40e-19 90.1
Msa1388550 AT1G26580.2 32.124 193 110 6 396 570 131 320 1.02e-18 89.7
Msa1388550 AT1G26580.1 32.124 193 110 6 396 570 131 320 1.02e-18 89.7
Msa1388550 AT2G03470.1 32.240 183 105 6 399 570 122 296 1.02e-17 86.7
Msa1388550 AT2G03470.2 32.240 183 105 6 399 570 121 295 1.06e-17 86.3
Msa1388550 AT1G13880.3 29.843 191 112 7 402 579 131 312 2.63e-16 82.0
Msa1388550 AT1G13880.1 29.843 191 112 7 402 579 130 311 2.66e-16 82.0
Msa1388550 AT1G13880.2 29.843 191 112 7 402 579 140 321 2.75e-16 82.0

Find 186 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCACTTAGTATCGCTTTC+AGG 0.202455 tig0021145:-37112 None:intergenic
TCAGGCATCGGAACAATTTC+AGG 0.206652 tig0021145:-35550 None:intergenic
AGAGAGGATATGAACATTTC+TGG 0.214678 tig0021145:+37413 Msa1388550:CDS
GTCTGGTTTGATGGGGTTTA+AGG 0.229952 tig0021145:+34946 Msa1388550:CDS
CTCCGACAATCTACAATCTT+AGG 0.256515 tig0021145:+36861 Msa1388550:CDS
GAAAGGTTGGTTTGTTGTTT+TGG 0.268550 tig0021145:+37791 Msa1388550:three_prime_UTR
GACGATCAAGTTATAGTAGA+TGG 0.274070 tig0021145:+37023 Msa1388550:CDS
CGAGCATTGAACAGCAAAAT+TGG 0.292781 tig0021145:+35659 Msa1388550:CDS
TATGGTATGCTTTGCGTTGC+AGG 0.295894 tig0021145:-37507 None:intergenic
GTGAGTTGTGGGATTTGGTT+GGG 0.298315 tig0021145:+34807 Msa1388550:CDS
TGATGGTGAGTTGTGGGATT+TGG 0.308604 tig0021145:+34802 Msa1388550:CDS
AAATGTTCATATCCTCTCTT+TGG 0.311222 tig0021145:-37409 None:intergenic
TGATAATATTGTTCCGGTAT+TGG 0.317593 tig0021145:+35420 Msa1388550:CDS
AAAGTTTCCCTGCTCTTGTT+AGG 0.326065 tig0021145:-37457 None:intergenic
AAAATATGATATGCAATCTA+TGG 0.330951 tig0021145:-37919 None:intergenic
CGTTGGTTCGACGCTGAAAC+TGG 0.334461 tig0021145:+34853 Msa1388550:CDS
CATCCCTCGATTCATCATTC+TGG 0.335310 tig0021145:-35221 None:intergenic
TGTGCTAGGCGAAGGAGATT+TGG 0.343980 tig0021145:+36955 Msa1388550:CDS
CCCTTTCCATGTGGCTACTC+TGG 0.347988 tig0021145:-37616 None:intergenic
TTAGTATCGCTTTCAGGATT+CGG 0.348247 tig0021145:-37106 None:intergenic
AAGGTTAAGGGTGGAGTTGA+TGG 0.350428 tig0021145:+35124 Msa1388550:CDS
GTTTGATGAGTCTGGTTTGA+TGG 0.354024 tig0021145:+34937 Msa1388550:CDS
GGTGAGTTGTGGGATTTGGT+TGG 0.366075 tig0021145:+34806 Msa1388550:CDS
CTGTCTACATTTATATATAG+TGG 0.366904 tig0021145:+37896 Msa1388550:three_prime_UTR
CAGGGTGGGAATGTGGTGGC+TGG 0.374383 tig0021145:+34995 Msa1388550:CDS
ATGAAGACGAGAAAAGGTTC+AGG 0.374801 tig0021145:+37365 Msa1388550:CDS
GTTGGGGAAGAATGTGGCTT+AGG 0.382436 tig0021145:+34824 Msa1388550:CDS
AAAATCAGAATTTGAAAGGT+TGG 0.383448 tig0021145:+37778 Msa1388550:three_prime_UTR
AGTGGTTTGGGACCCAAGTA+TGG 0.390417 tig0021145:+37128 Msa1388550:CDS
GCTAAGGTGTTTGATGAGTC+TGG 0.404181 tig0021145:+34929 Msa1388550:CDS
GAAAGCGATACTAAGTGGTT+TGG 0.405886 tig0021145:+37115 Msa1388550:CDS
TTTGATGAGTCTGGTTTGAT+GGG 0.408271 tig0021145:+34938 Msa1388550:CDS
CTTGACGAGCTTCGTGAAGA+TGG 0.408980 tig0021145:-36926 None:intergenic
CACGGTGGGAATGTGGTAAC+TGG 0.409472 tig0021145:+35097 Msa1388550:CDS
TCCCTAAGATTGTAGATTGT+CGG 0.412515 tig0021145:-36863 None:intergenic
AACTGAGGATGCGTCTGATT+TGG 0.415902 tig0021145:+35339 Msa1388550:CDS
GAATGAGTTGTTGTCGGGTC+AGG 0.421751 tig0021145:+34976 Msa1388550:CDS
TCATGCGGCTGCGCAGTTCC+AGG 0.424066 tig0021145:+37217 Msa1388550:CDS
TTCTCAATTTCTTGTCGAAA+GGG 0.425727 tig0021145:+37168 Msa1388550:CDS
TTACCATTGGCGTTTCGATA+AGG 0.427484 tig0021145:+37312 Msa1388550:CDS
TTGGATTTGTCTGGTGGTGA+TGG 0.430521 tig0021145:+35439 Msa1388550:CDS
TGTTTGTGATGGTGAGTTGT+GGG 0.431433 tig0021145:+34796 Msa1388550:CDS
TCCTGTCTTCCCCTTCCTAT+AGG 0.432461 tig0021145:-37193 None:intergenic
TCCTATAGGAAGGGGAAGAC+AGG 0.433625 tig0021145:+37192 Msa1388550:CDS
AAAGCGATACTAAGTGGTTT+GGG 0.433827 tig0021145:+37116 Msa1388550:CDS
TTGGTGAAGAGCGGTGAGTC+TGG 0.434679 tig0021145:+35358 Msa1388550:CDS
ATACAGCCAATGGCCATACT+TGG 0.437133 tig0021145:-37141 None:intergenic
AAACATTTCCTAACAAGAGC+AGG 0.439152 tig0021145:+37449 Msa1388550:CDS
GTGGAGGGCTTACAGTAATC+AGG 0.440528 tig0021145:+35582 Msa1388550:CDS
TGAGTTGTGGGATTTGGTTG+GGG 0.451450 tig0021145:+34808 Msa1388550:CDS
CTCTGTTTGATATGATGAGT+TGG 0.453588 tig0021145:+35497 Msa1388550:CDS
TTTGATGGGGTTTAAGGCTG+AGG 0.456932 tig0021145:+34952 Msa1388550:CDS
GTGAAGATGTTCGGAATTCG+TGG 0.464902 tig0021145:+37338 Msa1388550:CDS
GGTGGCTGGAGAGAAGGTTA+AGG 0.466762 tig0021145:+35009 Msa1388550:CDS
TATCGGTGATAATATTGTTC+CGG 0.468339 tig0021145:+35414 Msa1388550:CDS
GGTAACTGGAGAGAAGGTTA+AGG 0.476059 tig0021145:+35111 Msa1388550:CDS
GAGAGGATATGAACATTTCT+GGG 0.477135 tig0021145:+37414 Msa1388550:CDS
ATTCTCAATTTCTTGTCGAA+AGG 0.481791 tig0021145:+37167 Msa1388550:CDS
GTGGCTGGAGAGAAGGTTAA+GGG 0.482398 tig0021145:+35010 Msa1388550:CDS
GTTCCGGTATTGGATTTGTC+TGG 0.486514 tig0021145:+35430 Msa1388550:CDS
GGACAGATGAAGACGAGAAA+AGG 0.486764 tig0021145:+37359 Msa1388550:CDS
CTGACACATTCAACAGAACC+TGG 0.487585 tig0021145:-37235 None:intergenic
ATTGTTGTTGACGGGAAAGG+TGG 0.490249 tig0021145:+34764 Msa1388550:CDS
AACGCAAAGCATACCATAGC+AGG 0.490321 tig0021145:+37512 Msa1388550:CDS
TGTTCATATCCTCTCTTTGG+AGG 0.491805 tig0021145:-37406 None:intergenic
GAATCAAGAAATCCTACATC+GGG 0.494770 tig0021145:-35199 None:intergenic
CTGTTTGTGATGGTGAGTTG+TGG 0.494879 tig0021145:+34795 Msa1388550:CDS
GCATCGGCTCGCATCTGCGT+GGG 0.496198 tig0021145:+37046 Msa1388550:CDS
TCTGTTTGATATGATGAGTT+GGG 0.500139 tig0021145:+35498 Msa1388550:CDS
GAGAATCATGATGGTGATAT+CGG 0.501429 tig0021145:+35397 Msa1388550:CDS
TTGTCTGGTGGTGATGGAAG+TGG 0.503537 tig0021145:+35445 Msa1388550:CDS
GAGTCGTGTGTGATGAAAGT+TGG 0.506590 tig0021145:+34902 Msa1388550:CDS
TCCAGAGTAGCCACATGGAA+AGG 0.509426 tig0021145:+37615 Msa1388550:three_prime_UTR
GGGTTTGATGCTGTTTGTGA+TGG 0.509454 tig0021145:+34785 Msa1388550:CDS
TGGGACCCAAGTATGGCCAT+TGG 0.513214 tig0021145:+37135 Msa1388550:CDS
AGCTGTTGATTGTTGTTGAC+GGG 0.513550 tig0021145:+34756 Msa1388550:CDS
CCTCCTGTGTTGACAAATGT+TGG 0.518613 tig0021145:-37650 None:intergenic
ATAAGGTCGGTGAAGATGTT+CGG 0.518788 tig0021145:+37329 Msa1388550:CDS
CAAGTCGTCGTGCTGTGAAA+CGG 0.521739 tig0021145:+36987 Msa1388550:CDS
GTAACTGGAGAGAAGGTTAA+GGG 0.523633 tig0021145:+35112 Msa1388550:CDS
GCTAAAGCTAAGCTGGAGTT+AGG 0.528280 tig0021145:+37280 Msa1388550:CDS
GAATGTGGTGGCTGGAGAGA+AGG 0.530094 tig0021145:+35003 Msa1388550:CDS
TCCGACAATCTACAATCTTA+GGG 0.531884 tig0021145:+36862 Msa1388550:CDS
TGAGGTGAATGAGTTGTTGT+CGG 0.533812 tig0021145:+34970 Msa1388550:CDS
AAGGTTAAGGGTGGAGCTGA+TGG 0.534301 tig0021145:+35022 Msa1388550:CDS
GAAGTGGTTCTAGTCTTAAG+AGG 0.534929 tig0021145:+35461 Msa1388550:CDS
CATTGAACAGCAAAATTGGC+AGG 0.537300 tig0021145:+35663 Msa1388550:CDS
AATGAGTTGTTGTCGGGTCA+GGG 0.537370 tig0021145:+34977 Msa1388550:CDS
CCAGAGTAGCCACATGGAAA+GGG 0.539118 tig0021145:+37616 Msa1388550:three_prime_UTR
GATTTGGTTGGGGAAGAATG+TGG 0.539468 tig0021145:+34818 Msa1388550:CDS
CTGAGTGAGGGTAATGGTGA+TGG 0.540918 tig0021145:+35268 Msa1388550:CDS
CTTACAGTAATCAGGAGGCT+TGG 0.542653 tig0021145:+35590 Msa1388550:CDS
TAATCAGGAGGCTTGGAAGC+GGG 0.543149 tig0021145:+35597 Msa1388550:CDS
TGAGGGTAATGGTGATGGAA+TGG 0.545924 tig0021145:+35273 Msa1388550:CDS
GTGCGAATTTCGAGTCTAGT+GGG 0.546249 tig0021145:+35062 Msa1388550:CDS
GTGAAAGAAGTTCCCGATGT+AGG 0.550596 tig0021145:+35187 Msa1388550:CDS
AGTTCATCTGTGCTAGGCGA+AGG 0.552705 tig0021145:+36947 Msa1388550:CDS
GTCGGGTCAGGGTGGGAATG+TGG 0.553223 tig0021145:+34988 Msa1388550:CDS
TTACTGTAAGCCCTCCACTT+TGG 0.553928 tig0021145:-35577 None:intergenic
CTTGTCGAAAGGGATCCTAT+AGG 0.556633 tig0021145:+37178 Msa1388550:CDS
ACGATCAAGTTATAGTAGAT+GGG 0.556760 tig0021145:+37024 Msa1388550:CDS
GCGGGTTCTGTTGTTTCGAG+AGG 0.558329 tig0021145:+35615 Msa1388550:CDS
TGAAGACGAGAAAAGGTTCA+GGG 0.560005 tig0021145:+37366 Msa1388550:CDS
TTGATTGTTGTTGACGGGAA+AGG 0.563358 tig0021145:+34761 Msa1388550:CDS
GATGGAGGGAAAATAACTGT+AGG 0.564686 tig0021145:+35313 Msa1388550:CDS
GGATTCGGAGCAGTCCACTC+TGG 0.565588 tig0021145:-37091 None:intergenic
ATGGTCAGTGATACCGATGT+TGG 0.565824 tig0021145:+35241 Msa1388550:CDS
AAGCTGTTGATTGTTGTTGA+CGG 0.566769 tig0021145:+34755 Msa1388550:CDS
GAGGTGAATGAGTTGTTGTC+GGG 0.568676 tig0021145:+34971 Msa1388550:CDS
TCGTCAAGTTCATCTGTGCT+AGG 0.569671 tig0021145:+36941 Msa1388550:CDS
CAATCTACAATCTTAGGGAG+AGG 0.570505 tig0021145:+36867 Msa1388550:CDS
CCACCAGACAAATCCAATAC+CGG 0.573971 tig0021145:-35433 None:intergenic
GCCTGAAAAGCCAAAGTGGA+GGG 0.574127 tig0021145:+35567 Msa1388550:CDS
AGAAATTCACGGTGGGAATG+TGG 0.576328 tig0021145:+35090 Msa1388550:CDS
TACAGCCAATGGCCATACTT+GGG 0.577673 tig0021145:-37140 None:intergenic
GTGGGATCATCATACATGCT+AGG 0.577985 tig0021145:-36839 None:intergenic
TTGTTGTTGACGGGAAAGGT+GGG 0.578023 tig0021145:+34765 Msa1388550:CDS
CATCGGCTCGCATCTGCGTG+GGG 0.579313 tig0021145:+37047 Msa1388550:CDS
ACTGGAGAGAAGGTTAAGGG+TGG 0.579796 tig0021145:+35115 Msa1388550:CDS
ATCTACAATCTTAGGGAGAG+GGG 0.580993 tig0021145:+36869 Msa1388550:CDS
AATCTACAATCTTAGGGAGA+GGG 0.581096 tig0021145:+36868 Msa1388550:CDS
GGAAAGCTGAGTGAGGGTAA+TGG 0.581453 tig0021145:+35262 Msa1388550:CDS
CCGGTATTGGATTTGTCTGG+TGG 0.582583 tig0021145:+35433 Msa1388550:CDS
AAGTTATAGTAGATGGGCAT+CGG 0.582631 tig0021145:+37030 Msa1388550:CDS
TACCATAGCAGGCATACACT+TGG 0.584083 tig0021145:+37523 Msa1388550:CDS
AACATTTCCTAACAAGAGCA+GGG 0.584542 tig0021145:+37450 Msa1388550:CDS
CGACCTTATCGAAACGCCAA+TGG 0.585564 tig0021145:-37315 None:intergenic
CTCACTCAGCTTTCCAACAT+CGG 0.585605 tig0021145:-35254 None:intergenic
ATGGAGGGAAAATAACTGTA+GGG 0.586968 tig0021145:+35314 Msa1388550:CDS
GTTCATATCCTCTCTTTGGA+GGG 0.587128 tig0021145:-37405 None:intergenic
TCGAAAGGGATCCTATAGGA+AGG 0.587509 tig0021145:+37182 Msa1388550:CDS
CGATGCCTGAAAAGCCAAAG+TGG 0.588222 tig0021145:+35563 Msa1388550:CDS
TTGATGAGTCTGGTTTGATG+GGG 0.588528 tig0021145:+34939 Msa1388550:CDS
GGGTCAGGGTGGGAATGTGG+TGG 0.588589 tig0021145:+34991 Msa1388550:CDS
GAATGTGGTAACTGGAGAGA+AGG 0.588966 tig0021145:+35105 Msa1388550:CDS
TTTCACAGCACGACGACTTG+TGG 0.590229 tig0021145:-36984 None:intergenic
GAAAGGGATCCTATAGGAAG+GGG 0.590844 tig0021145:+37184 Msa1388550:CDS
ATCCTGAAAGCGATACTAAG+TGG 0.591141 tig0021145:+37110 Msa1388550:CDS
GGAATCAAGAAATCCTACAT+CGG 0.595001 tig0021145:-35200 None:intergenic
CGAAAGGGATCCTATAGGAA+GGG 0.595912 tig0021145:+37183 Msa1388550:CDS
AGTTGTTGTCGGGTCAGGGT+GGG 0.598266 tig0021145:+34981 Msa1388550:CDS
TCATCACTTGGTGACTACAA+AGG 0.599966 tig0021145:+37949 Msa1388550:three_prime_UTR
GGCATCGGCTCGCATCTGCG+TGG 0.600636 tig0021145:+37045 Msa1388550:CDS
GTAATCAGGAGGCTTGGAAG+CGG 0.600886 tig0021145:+35596 Msa1388550:CDS
GAGTCTGGATTGCTGAATGT+TGG 0.602152 tig0021145:+35373 Msa1388550:CDS
AGATTGTAGATTGTCGGAGT+GGG 0.602178 tig0021145:-36857 None:intergenic
CACCAAGTGTATGCCTGCTA+TGG 0.604240 tig0021145:-37525 None:intergenic
ATTCCAGAATGATGAATCGA+GGG 0.604352 tig0021145:+35218 Msa1388550:CDS
TAGTCTTAAGAGGAAGAGAG+AGG 0.607309 tig0021145:+35471 Msa1388550:CDS
CATTGGCGTTTCGATAAGGT+CGG 0.607511 tig0021145:+37316 Msa1388550:CDS
AGTGGGGAGAAAGAAATTCA+CGG 0.609700 tig0021145:+35079 Msa1388550:CDS
CGATGTTGGAAAGCTGAGTG+AGG 0.609819 tig0021145:+35255 Msa1388550:CDS
TAGGCGAAGGAGATTTGGAC+GGG 0.612279 tig0021145:+36960 Msa1388550:CDS
AGTGCGAATTTCGAGTCTAG+TGG 0.613148 tig0021145:+35061 Msa1388550:CDS
GATTCCAGAATGATGAATCG+AGG 0.616855 tig0021145:+35217 Msa1388550:CDS
AGCTAAGCTGGAGTTAGGCG+TGG 0.617435 tig0021145:+37285 Msa1388550:CDS
GATGAAAGTTGGTGATGCTA+AGG 0.617892 tig0021145:+34913 Msa1388550:CDS
TGCCTGAAAAGCCAAAGTGG+AGG 0.624771 tig0021145:+35566 Msa1388550:CDS
CAGCTTTACCCTCCAAAGAG+AGG 0.627360 tig0021145:+37397 Msa1388550:CDS
GGAACAATTTCAGGATCACA+AGG 0.631056 tig0021145:-35541 None:intergenic
CAGAATGATGAATCGAGGGA+TGG 0.631748 tig0021145:+35222 Msa1388550:CDS
TCTACAATCTTAGGGAGAGG+GGG 0.633262 tig0021145:+36870 Msa1388550:CDS
ATCAAGCTGAAGTGCCAGAG+TGG 0.634304 tig0021145:+37077 Msa1388550:CDS
TCTTAAGAGGAAGAGAGAGG+AGG 0.635943 tig0021145:+35474 Msa1388550:CDS
CCAACATTTGTCAACACAGG+AGG 0.637631 tig0021145:+37650 Msa1388550:three_prime_UTR
AATCCAACATTTGTCAACAC+AGG 0.638035 tig0021145:+37647 Msa1388550:three_prime_UTR
CTAGGCGAAGGAGATTTGGA+CGG 0.640028 tig0021145:+36959 Msa1388550:CDS
AAGATTGTAGATTGTCGGAG+TGG 0.640461 tig0021145:-36858 None:intergenic
GAGTTGTTGTCGGGTCAGGG+TGG 0.642746 tig0021145:+34980 Msa1388550:CDS
TGAGAATTCGATACAGCCAA+TGG 0.643809 tig0021145:-37151 None:intergenic
GGGAGAAAGAAATTCACGGT+GGG 0.651200 tig0021145:+35083 Msa1388550:CDS
GATGTTGGAAAGCTGAGTGA+GGG 0.661886 tig0021145:+35256 Msa1388550:CDS
TAGATTCCAGAGTAGCCACA+TGG 0.665441 tig0021145:+37610 Msa1388550:exon
TGCGAATTTCGAGTCTAGTG+GGG 0.671840 tig0021145:+35063 Msa1388550:CDS
TGTGGCTTAGGTGTTAACGT+TGG 0.673988 tig0021145:+34836 Msa1388550:CDS
AGGGGAAGACAGGATTCATG+CGG 0.683109 tig0021145:+37202 Msa1388550:CDS
AATGTTGGCGAGAATCATGA+TGG 0.684957 tig0021145:+35388 Msa1388550:CDS
TGGAGCTGATGGTGCTAACG+AGG 0.694652 tig0021145:+35033 Msa1388550:CDS
GCTGGAGAGAAGGTTAAGGG+TGG 0.695646 tig0021145:+35013 Msa1388550:CDS
TGGAGTTGATGGTGCTAACG+AGG 0.697135 tig0021145:+35135 Msa1388550:CDS
GCGTCTGATTTGGTGAAGAG+CGG 0.702691 tig0021145:+35349 Msa1388550:CDS
GAGGGCTTACAGTAATCAGG+AGG 0.703144 tig0021145:+35585 Msa1388550:CDS
AGGATCACAAGGGTTACTCG+CGG 0.705433 tig0021145:-35530 None:intergenic
GAACAATTTCAGGATCACAA+GGG 0.713883 tig0021145:-35540 None:intergenic
AATAACTGTAGGGACAACTG+AGG 0.724458 tig0021145:+35324 Msa1388550:CDS
GGGGAGAAAGAAATTCACGG+TGG 0.772176 tig0021145:+35082 Msa1388550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAAGCAATTTAAGTT+TGG - tig0021145:35724-35743 None:intergenic 15.0%
!!! ATATACATCATAGATTTTAA+AGG + tig0021145:37751-37770 Msa1388550:three_prime_UTR 15.0%
!!! TATACATCATAGATTTTAAA+GGG + tig0021145:37752-37771 Msa1388550:three_prime_UTR 15.0%
!! TTTCATGTGTAAATGATAAT+TGG + tig0021145:35922-35941 Msa1388550:intron 20.0%
!!! TCTTATTTTGAGCAAATAAT+TGG + tig0021145:35954-35973 Msa1388550:intron 20.0%
!! ATGACTTATTAAATTTCATG+TGG + tig0021145:36102-36121 Msa1388550:intron 20.0%
!! TGACTTATTAAATTTCATGT+GGG + tig0021145:36103-36122 Msa1388550:intron 20.0%
!! ACAAAATTAAATCATGATGA+AGG - tig0021145:36229-36248 None:intergenic 20.0%
!!! TGTTTTTAGTTAGTTAATGT+TGG + tig0021145:36432-36451 Msa1388550:intron 20.0%
!!! GTTTTTAGTTAGTTAATGTT+GGG + tig0021145:36433-36452 Msa1388550:intron 20.0%
!!! TGTTGTTGTTATATTAAAAG+TGG + tig0021145:36517-36536 Msa1388550:intron 20.0%
!!! AACCATGCTTTAAATAAAAA+GGG - tig0021145:36570-36589 None:intergenic 20.0%
!!! TAACCATGCTTTAAATAAAA+AGG - tig0021145:36571-36590 None:intergenic 20.0%
!!! CGTATTTTTTAGAGTAATAA+AGG + tig0021145:36696-36715 Msa1388550:intron 20.0%
!! GTTAAAAATCAGAATTTGAA+AGG + tig0021145:37774-37793 Msa1388550:three_prime_UTR 20.0%
!! AAAATATGATATGCAATCTA+TGG - tig0021145:37922-37941 None:intergenic 20.0%
!!! ATTTTCATTTTTTCATCACT+TGG + tig0021145:37937-37956 Msa1388550:three_prime_UTR 20.0%
! TGCTTGAAATTTAGTAAACT+TGG + tig0021145:35764-35783 Msa1388550:intron 25.0%
! GCTTGAAATTTAGTAAACTT+GGG + tig0021145:35765-35784 Msa1388550:intron 25.0%
! AACAGTTGACATTAAAACTT+GGG - tig0021145:36025-36044 None:intergenic 25.0%
! TAACAGTTGACATTAAAACT+TGG - tig0021145:36026-36045 None:intergenic 25.0%
! GAAATTTAATAAGTCATCAG+TGG - tig0021145:36099-36118 None:intergenic 25.0%
!!! AATATCTCAGCGTTTTTTTT+CGG - tig0021145:36353-36372 None:intergenic 25.0%
!! GAGATATTGTCATAGTATTT+TGG + tig0021145:36365-36384 Msa1388550:intron 25.0%
! ATTCAGTTTCCTTACTATTA+AGG + tig0021145:36486-36505 Msa1388550:intron 25.0%
! AATAACACACCTTAATAGTA+AGG - tig0021145:36498-36517 None:intergenic 25.0%
! TTGTTATATTAAAAGTGGCA+AGG + tig0021145:36522-36541 Msa1388550:intron 25.0%
! ATGGTTAACTTATGCATTTA+TGG + tig0021145:36584-36603 Msa1388550:intron 25.0%
! TGGTTAACTTATGCATTTAT+GGG + tig0021145:36585-36604 Msa1388550:intron 25.0%
! AAGTATCTAAATTGAATTGC+AGG + tig0021145:36658-36677 Msa1388550:intron 25.0%
! AGTAATAAAGGTTTATTAGC+TGG + tig0021145:36708-36727 Msa1388550:intron 25.0%
! GTTAATGCAAAAGGAAATTT+AGG - tig0021145:36779-36798 None:intergenic 25.0%
!! TGCAAAATTATACAAGGTTT+TGG - tig0021145:36803-36822 None:intergenic 25.0%
! AAAATCAGAATTTGAAAGGT+TGG + tig0021145:37778-37797 Msa1388550:three_prime_UTR 25.0%
! CTGTCTACATTTATATATAG+TGG + tig0021145:37896-37915 Msa1388550:three_prime_UTR 25.0%
!!! AGAAGCTTTTTAGAAATACA+GGG + tig0021145:37983-38002 Msa1388550:three_prime_UTR 25.0%
! GAAGTAGTTGAAGATTTTGA+TGG + tig0021145:35295-35314 Msa1388550:CDS 30.0%
!! TAGTTGAAGATTTTGATGGA+GGG + tig0021145:35299-35318 Msa1388550:CDS 30.0%
TATCGGTGATAATATTGTTC+CGG + tig0021145:35414-35433 Msa1388550:CDS 30.0%
TGATAATATTGTTCCGGTAT+TGG + tig0021145:35420-35439 Msa1388550:CDS 30.0%
TCTGTTTGATATGATGAGTT+GGG + tig0021145:35498-35517 Msa1388550:CDS 30.0%
! CAATTTAAGTTTGGCTAACT+CGG - tig0021145:35715-35734 None:intergenic 30.0%
! TGTAATTGTTCTGTTTCTCA+AGG + tig0021145:35890-35909 Msa1388550:intron 30.0%
!! TACAGTTTCATTTAGTTTGC+TGG + tig0021145:36044-36063 Msa1388550:intron 30.0%
!! ACAGTTTCATTTAGTTTGCT+GGG + tig0021145:36045-36064 Msa1388550:intron 30.0%
ATGCTAATGTGATGTGTATT+TGG + tig0021145:36281-36300 Msa1388550:intron 30.0%
TGCTAATGTGATGTGTATTT+GGG + tig0021145:36282-36301 Msa1388550:intron 30.0%
TAAATAAAAAGGGCCCATAA+TGG - tig0021145:36560-36579 None:intergenic 30.0%
AATGTATGCTATCCCAAAAA+GGG - tig0021145:36611-36630 None:intergenic 30.0%
TGACTCTGCAAAATTATACA+AGG - tig0021145:36809-36828 None:intergenic 30.0%
ACGATCAAGTTATAGTAGAT+GGG + tig0021145:37024-37043 Msa1388550:CDS 30.0%
ATTCTCAATTTCTTGTCGAA+AGG + tig0021145:37167-37186 Msa1388550:CDS 30.0%
TTCTCAATTTCTTGTCGAAA+GGG + tig0021145:37168-37187 Msa1388550:CDS 30.0%
!!! TAGCTTTTTTCTCAGAAATG+TGG - tig0021145:37264-37283 None:intergenic 30.0%
AAATGTTCATATCCTCTCTT+TGG - tig0021145:37412-37431 None:intergenic 30.0%
!! TCTGATTTGAAGAAGCTTTT+TGG + tig0021145:37577-37596 Msa1388550:CDS 30.0%
! TTCTAAAAAGCTTCTGTTTC+AGG - tig0021145:37978-37997 None:intergenic 30.0%
!! CAGAAGCTTTTTAGAAATAC+AGG + tig0021145:37982-38001 Msa1388550:three_prime_UTR 30.0%
! ACAGGGAAATTTTATTGTAG+AGG + tig0021145:38000-38019 Msa1388550:three_prime_UTR 30.0%
! CAGGGAAATTTTATTGTAGA+GGG + tig0021145:38001-38020 Msa1388550:three_prime_UTR 30.0%
! AAGCTGTTGATTGTTGTTGA+CGG + tig0021145:34755-34774 Msa1388550:CDS 35.0%
! TTTGATGAGTCTGGTTTGAT+GGG + tig0021145:34938-34957 Msa1388550:CDS 35.0%
!! AAGAGTGTGAATTTTGAGTC+TGG + tig0021145:35160-35179 Msa1388550:CDS 35.0%
GAATCAAGAAATCCTACATC+GGG - tig0021145:35202-35221 None:intergenic 35.0%
GGAATCAAGAAATCCTACAT+CGG - tig0021145:35203-35222 None:intergenic 35.0%
ATTCCAGAATGATGAATCGA+GGG + tig0021145:35218-35237 Msa1388550:CDS 35.0%
!! GTAGTTGAAGATTTTGATGG+AGG + tig0021145:35298-35317 Msa1388550:CDS 35.0%
ATGGAGGGAAAATAACTGTA+GGG + tig0021145:35314-35333 Msa1388550:CDS 35.0%
! GAGAATCATGATGGTGATAT+CGG + tig0021145:35397-35416 Msa1388550:CDS 35.0%
CTCTGTTTGATATGATGAGT+TGG + tig0021145:35497-35516 Msa1388550:CDS 35.0%
GAACAATTTCAGGATCACAA+GGG - tig0021145:35543-35562 None:intergenic 35.0%
! GCATTTGTTAGTTGTTACTG+TGG + tig0021145:35979-35998 Msa1388550:intron 35.0%
!! CAGTTTCATTTAGTTTGCTG+GGG + tig0021145:36046-36065 Msa1388550:intron 35.0%
!! AGTTTCATTTAGTTTGCTGG+GGG + tig0021145:36047-36066 Msa1388550:intron 35.0%
! CACTTGTCAGTTTTTCATGT+GGG + tig0021145:36128-36147 Msa1388550:intron 35.0%
! ACTTGTCAGTTTTTCATGTG+GGG + tig0021145:36129-36148 Msa1388550:intron 35.0%
TAATGTGATGTGTATTTGGG+TGG + tig0021145:36285-36304 Msa1388550:intron 35.0%
AATGTGATGTGTATTTGGGT+GGG + tig0021145:36286-36305 Msa1388550:intron 35.0%
GCAAACATTCTCACCATTAT+GGG + tig0021145:36544-36563 Msa1388550:intron 35.0%
!! GGCCCTTTTTATTTAAAGCA+TGG + tig0021145:36565-36584 Msa1388550:intron 35.0%
CAATGTATGCTATCCCAAAA+AGG - tig0021145:36612-36631 None:intergenic 35.0%
!!! GGTTTTGGTGTTAATGCAAA+AGG - tig0021145:36788-36807 None:intergenic 35.0%
TCCGACAATCTACAATCTTA+GGG + tig0021145:36862-36881 Msa1388550:CDS 35.0%
TCCCTAAGATTGTAGATTGT+CGG - tig0021145:36866-36885 None:intergenic 35.0%
AATCTACAATCTTAGGGAGA+GGG + tig0021145:36868-36887 Msa1388550:CDS 35.0%
GACGATCAAGTTATAGTAGA+TGG + tig0021145:37023-37042 Msa1388550:CDS 35.0%
AAGTTATAGTAGATGGGCAT+CGG + tig0021145:37030-37049 Msa1388550:CDS 35.0%
TTAGTATCGCTTTCAGGATT+CGG - tig0021145:37109-37128 None:intergenic 35.0%
AAAGCGATACTAAGTGGTTT+GGG + tig0021145:37116-37135 Msa1388550:CDS 35.0%
GAAAAAAGCTAAAGCTAAGC+TGG + tig0021145:37273-37292 Msa1388550:CDS 35.0%
AGAGAGGATATGAACATTTC+TGG + tig0021145:37413-37432 Msa1388550:CDS 35.0%
GAGAGGATATGAACATTTCT+GGG + tig0021145:37414-37433 Msa1388550:CDS 35.0%
AAACATTTCCTAACAAGAGC+AGG + tig0021145:37449-37468 Msa1388550:CDS 35.0%
AACATTTCCTAACAAGAGCA+GGG + tig0021145:37450-37469 Msa1388550:CDS 35.0%
AATCCAACATTTGTCAACAC+AGG + tig0021145:37647-37666 Msa1388550:three_prime_UTR 35.0%
!!! GAAAGGTTGGTTTGTTGTTT+TGG + tig0021145:37791-37810 Msa1388550:three_prime_UTR 35.0%
!!! TTTGTTGTTTTGGTCATGTC+TGG + tig0021145:37801-37820 Msa1388550:three_prime_UTR 35.0%
! CTAGTATGCTTCAGATTTTG+AGG + tig0021145:37861-37880 Msa1388550:three_prime_UTR 35.0%
! AGCTGTTGATTGTTGTTGAC+GGG + tig0021145:34756-34775 Msa1388550:CDS 40.0%
! TTGATTGTTGTTGACGGGAA+AGG + tig0021145:34761-34780 Msa1388550:CDS 40.0%
! TGTTTGTGATGGTGAGTTGT+GGG + tig0021145:34796-34815 Msa1388550:CDS 40.0%
!! GATGAAAGTTGGTGATGCTA+AGG + tig0021145:34913-34932 Msa1388550:CDS 40.0%
! GTTTGATGAGTCTGGTTTGA+TGG + tig0021145:34937-34956 Msa1388550:CDS 40.0%
! TTGATGAGTCTGGTTTGATG+GGG + tig0021145:34939-34958 Msa1388550:CDS 40.0%
TGAGGTGAATGAGTTGTTGT+CGG + tig0021145:34970-34989 Msa1388550:CDS 40.0%
AGTGGGGAGAAAGAAATTCA+CGG + tig0021145:35079-35098 Msa1388550:CDS 40.0%
GTAACTGGAGAGAAGGTTAA+GGG + tig0021145:35112-35131 Msa1388550:CDS 40.0%
!! AGTGTGAATTTTGAGTCTGG+TGG + tig0021145:35163-35182 Msa1388550:CDS 40.0%
!! GTGTGAATTTTGAGTCTGGT+GGG + tig0021145:35164-35183 Msa1388550:CDS 40.0%
!! TGTGAATTTTGAGTCTGGTG+GGG + tig0021145:35165-35184 Msa1388550:CDS 40.0%
GATTCCAGAATGATGAATCG+AGG + tig0021145:35217-35236 Msa1388550:CDS 40.0%
GATGGAGGGAAAATAACTGT+AGG + tig0021145:35313-35332 Msa1388550:CDS 40.0%
AATAACTGTAGGGACAACTG+AGG + tig0021145:35324-35343 Msa1388550:CDS 40.0%
AATGTTGGCGAGAATCATGA+TGG + tig0021145:35388-35407 Msa1388550:CDS 40.0%
!! GAAGTGGTTCTAGTCTTAAG+AGG + tig0021145:35461-35480 Msa1388550:CDS 40.0%
! TAGTCTTAAGAGGAAGAGAG+AGG + tig0021145:35471-35490 Msa1388550:CDS 40.0%
GGAACAATTTCAGGATCACA+AGG - tig0021145:35544-35563 None:intergenic 40.0%
!! CGAGCATTGAACAGCAAAAT+TGG + tig0021145:35659-35678 Msa1388550:CDS 40.0%
CATTGAACAGCAAAATTGGC+AGG + tig0021145:35663-35682 Msa1388550:CDS 40.0%
!! GTTTCATTTAGTTTGCTGGG+GGG + tig0021145:36048-36067 Msa1388550:intron 40.0%
CCACATGAAAAACTGACAAG+TGG - tig0021145:36130-36149 None:intergenic 40.0%
! CCACTTGTCAGTTTTTCATG+TGG + tig0021145:36127-36146 Msa1388550:intron 40.0%
ATGTGATGTGTATTTGGGTG+GGG + tig0021145:36287-36306 Msa1388550:intron 40.0%
ATTTGGGTGGGGAAAATTGT+TGG + tig0021145:36298-36317 Msa1388550:intron 40.0%
GGCAAACATTCTCACCATTA+TGG + tig0021145:36543-36562 Msa1388550:intron 40.0%
! ATGCATTTATGGGCCCTTTT+TGG + tig0021145:36595-36614 Msa1388550:intron 40.0%
! TGCATTTATGGGCCCTTTTT+GGG + tig0021145:36596-36615 Msa1388550:intron 40.0%
!! GATAGCATACATTGACTTGC+AGG + tig0021145:36618-36637 Msa1388550:intron 40.0%
AGATTGTAGATTGTCGGAGT+GGG - tig0021145:36860-36879 None:intergenic 40.0%
AAGATTGTAGATTGTCGGAG+TGG - tig0021145:36861-36880 None:intergenic 40.0%
CTCCGACAATCTACAATCTT+AGG + tig0021145:36861-36880 Msa1388550:CDS 40.0%
CAATCTACAATCTTAGGGAG+AGG + tig0021145:36867-36886 Msa1388550:CDS 40.0%
ATCTACAATCTTAGGGAGAG+GGG + tig0021145:36869-36888 Msa1388550:CDS 40.0%
ATCCTGAAAGCGATACTAAG+TGG + tig0021145:37110-37129 Msa1388550:CDS 40.0%
AACCACTTAGTATCGCTTTC+AGG - tig0021145:37115-37134 None:intergenic 40.0%
GAAAGCGATACTAAGTGGTT+TGG + tig0021145:37115-37134 Msa1388550:CDS 40.0%
TGAGAATTCGATACAGCCAA+TGG - tig0021145:37154-37173 None:intergenic 40.0%
TTACCATTGGCGTTTCGATA+AGG + tig0021145:37312-37331 Msa1388550:CDS 40.0%
ATAAGGTCGGTGAAGATGTT+CGG + tig0021145:37329-37348 Msa1388550:CDS 40.0%
ATGAAGACGAGAAAAGGTTC+AGG + tig0021145:37365-37384 Msa1388550:CDS 40.0%
TGAAGACGAGAAAAGGTTCA+GGG + tig0021145:37366-37385 Msa1388550:CDS 40.0%
GTTCATATCCTCTCTTTGGA+GGG - tig0021145:37408-37427 None:intergenic 40.0%
TGTTCATATCCTCTCTTTGG+AGG - tig0021145:37409-37428 None:intergenic 40.0%
AAAGTTTCCCTGCTCTTGTT+AGG - tig0021145:37460-37479 None:intergenic 40.0%
! TGAAGAAGCTTTTTGGACGT+CGG + tig0021145:37584-37603 Msa1388550:CDS 40.0%
!!! TGCTATTTTCCCTTTCCATG+TGG - tig0021145:37628-37647 None:intergenic 40.0%
! TCATCACTTGGTGACTACAA+AGG + tig0021145:37949-37968 Msa1388550:three_prime_UTR 40.0%
! ATTGTTGTTGACGGGAAAGG+TGG + tig0021145:34764-34783 Msa1388550:CDS 45.0%
! TTGTTGTTGACGGGAAAGGT+GGG + tig0021145:34765-34784 Msa1388550:CDS 45.0%
! GGGTTTGATGCTGTTTGTGA+TGG + tig0021145:34785-34804 Msa1388550:CDS 45.0%
! CTGTTTGTGATGGTGAGTTG+TGG + tig0021145:34795-34814 Msa1388550:CDS 45.0%
! TGATGGTGAGTTGTGGGATT+TGG + tig0021145:34802-34821 Msa1388550:CDS 45.0%
! GTGAGTTGTGGGATTTGGTT+GGG + tig0021145:34807-34826 Msa1388550:CDS 45.0%
! TGAGTTGTGGGATTTGGTTG+GGG + tig0021145:34808-34827 Msa1388550:CDS 45.0%
! GATTTGGTTGGGGAAGAATG+TGG + tig0021145:34818-34837 Msa1388550:CDS 45.0%
TGTGGCTTAGGTGTTAACGT+TGG + tig0021145:34836-34855 Msa1388550:CDS 45.0%
GAGTCGTGTGTGATGAAAGT+TGG + tig0021145:34902-34921 Msa1388550:CDS 45.0%
! GCTAAGGTGTTTGATGAGTC+TGG + tig0021145:34929-34948 Msa1388550:CDS 45.0%
! GTCTGGTTTGATGGGGTTTA+AGG + tig0021145:34946-34965 Msa1388550:CDS 45.0%
! TTTGATGGGGTTTAAGGCTG+AGG + tig0021145:34952-34971 Msa1388550:CDS 45.0%
GAGGTGAATGAGTTGTTGTC+GGG + tig0021145:34971-34990 Msa1388550:CDS 45.0%
AATGAGTTGTTGTCGGGTCA+GGG + tig0021145:34977-34996 Msa1388550:CDS 45.0%
! AGTGCGAATTTCGAGTCTAG+TGG + tig0021145:35061-35080 Msa1388550:CDS 45.0%
! GTGCGAATTTCGAGTCTAGT+GGG + tig0021145:35062-35081 Msa1388550:CDS 45.0%
! TGCGAATTTCGAGTCTAGTG+GGG + tig0021145:35063-35082 Msa1388550:CDS 45.0%
GGGAGAAAGAAATTCACGGT+GGG + tig0021145:35083-35102 Msa1388550:CDS 45.0%
AGAAATTCACGGTGGGAATG+TGG + tig0021145:35090-35109 Msa1388550:CDS 45.0%
GAATGTGGTAACTGGAGAGA+AGG + tig0021145:35105-35124 Msa1388550:CDS 45.0%
GGTAACTGGAGAGAAGGTTA+AGG + tig0021145:35111-35130 Msa1388550:CDS 45.0%
AAGGTTAAGGGTGGAGTTGA+TGG + tig0021145:35124-35143 Msa1388550:CDS 45.0%
GTGAAAGAAGTTCCCGATGT+AGG + tig0021145:35187-35206 Msa1388550:CDS 45.0%
CATCCCTCGATTCATCATTC+TGG - tig0021145:35224-35243 None:intergenic 45.0%
CAGAATGATGAATCGAGGGA+TGG + tig0021145:35222-35241 Msa1388550:CDS 45.0%
ATGGTCAGTGATACCGATGT+TGG + tig0021145:35241-35260 Msa1388550:CDS 45.0%
CTCACTCAGCTTTCCAACAT+CGG - tig0021145:35257-35276 None:intergenic 45.0%
GATGTTGGAAAGCTGAGTGA+GGG + tig0021145:35256-35275 Msa1388550:CDS 45.0%
!! TGAGGGTAATGGTGATGGAA+TGG + tig0021145:35273-35292 Msa1388550:CDS 45.0%
AACTGAGGATGCGTCTGATT+TGG + tig0021145:35339-35358 Msa1388550:CDS 45.0%
GAGTCTGGATTGCTGAATGT+TGG + tig0021145:35373-35392 Msa1388550:CDS 45.0%
! GTTCCGGTATTGGATTTGTC+TGG + tig0021145:35430-35449 Msa1388550:CDS 45.0%
CCACCAGACAAATCCAATAC+CGG - tig0021145:35436-35455 None:intergenic 45.0%
! TTGGATTTGTCTGGTGGTGA+TGG + tig0021145:35439-35458 Msa1388550:CDS 45.0%
TCTTAAGAGGAAGAGAGAGG+AGG + tig0021145:35474-35493 Msa1388550:CDS 45.0%
!! TCAGGCATCGGAACAATTTC+AGG - tig0021145:35553-35572 None:intergenic 45.0%
!!! CACTTTGGCTTTTCAGGCAT+CGG - tig0021145:35565-35584 None:intergenic 45.0%
TTACTGTAAGCCCTCCACTT+TGG - tig0021145:35580-35599 None:intergenic 45.0%
CTTACAGTAATCAGGAGGCT+TGG + tig0021145:35590-35609 Msa1388550:CDS 45.0%
GTGGGATCATCATACATGCT+AGG - tig0021145:36842-36861 None:intergenic 45.0%
TCTACAATCTTAGGGAGAGG+GGG + tig0021145:36870-36889 Msa1388550:CDS 45.0%
TCGTCAAGTTCATCTGTGCT+AGG + tig0021145:36941-36960 Msa1388550:CDS 45.0%
TACAGCCAATGGCCATACTT+GGG - tig0021145:37143-37162 None:intergenic 45.0%
ATACAGCCAATGGCCATACT+TGG - tig0021145:37144-37163 None:intergenic 45.0%
CTTGTCGAAAGGGATCCTAT+AGG + tig0021145:37178-37197 Msa1388550:CDS 45.0%
TCGAAAGGGATCCTATAGGA+AGG + tig0021145:37182-37201 Msa1388550:CDS 45.0%
CGAAAGGGATCCTATAGGAA+GGG + tig0021145:37183-37202 Msa1388550:CDS 45.0%
GAAAGGGATCCTATAGGAAG+GGG + tig0021145:37184-37203 Msa1388550:CDS 45.0%
CTGACACATTCAACAGAACC+TGG - tig0021145:37238-37257 None:intergenic 45.0%
GCTAAAGCTAAGCTGGAGTT+AGG + tig0021145:37280-37299 Msa1388550:CDS 45.0%
! TAGGCGTGGCTTTTTACCAT+TGG + tig0021145:37299-37318 Msa1388550:CDS 45.0%
CATTGGCGTTTCGATAAGGT+CGG + tig0021145:37316-37335 Msa1388550:CDS 45.0%
GTGAAGATGTTCGGAATTCG+TGG + tig0021145:37338-37357 Msa1388550:CDS 45.0%
GGACAGATGAAGACGAGAAA+AGG + tig0021145:37359-37378 Msa1388550:CDS 45.0%
TATGGTATGCTTTGCGTTGC+AGG - tig0021145:37510-37529 None:intergenic 45.0%
AACGCAAAGCATACCATAGC+AGG + tig0021145:37512-37531 Msa1388550:CDS 45.0%
TACCATAGCAGGCATACACT+TGG + tig0021145:37523-37542 Msa1388550:CDS 45.0%
! GAAGAAGCTTTTTGGACGTC+GGG + tig0021145:37585-37604 Msa1388550:CDS 45.0%
! AAGAAGCTTTTTGGACGTCG+GGG + tig0021145:37586-37605 Msa1388550:CDS 45.0%
TAGATTCCAGAGTAGCCACA+TGG + tig0021145:37610-37629 Msa1388550:exon 45.0%
! CCTCCTGTGTTGACAAATGT+TGG - tig0021145:37653-37672 None:intergenic 45.0%
CCAACATTTGTCAACACAGG+AGG + tig0021145:37650-37669 Msa1388550:three_prime_UTR 45.0%
!! GTTCGTGTTTTGAACAGCGT+AGG + tig0021145:37827-37846 Msa1388550:three_prime_UTR 45.0%
! GGTGAGTTGTGGGATTTGGT+TGG + tig0021145:34806-34825 Msa1388550:CDS 50.0%
GTTGGGGAAGAATGTGGCTT+AGG + tig0021145:34824-34843 Msa1388550:CDS 50.0%
GAATGAGTTGTTGTCGGGTC+AGG + tig0021145:34976-34995 Msa1388550:CDS 50.0%
GTGGCTGGAGAGAAGGTTAA+GGG + tig0021145:35010-35029 Msa1388550:CDS 50.0%
AAGGTTAAGGGTGGAGCTGA+TGG + tig0021145:35022-35041 Msa1388550:CDS 50.0%
GGGGAGAAAGAAATTCACGG+TGG + tig0021145:35082-35101 Msa1388550:CDS 50.0%
ACTGGAGAGAAGGTTAAGGG+TGG + tig0021145:35115-35134 Msa1388550:CDS 50.0%
!! TGGAGTTGATGGTGCTAACG+AGG + tig0021145:35135-35154 Msa1388550:CDS 50.0%
CGATGTTGGAAAGCTGAGTG+AGG + tig0021145:35255-35274 Msa1388550:CDS 50.0%
GGAAAGCTGAGTGAGGGTAA+TGG + tig0021145:35262-35281 Msa1388550:CDS 50.0%
! CTGAGTGAGGGTAATGGTGA+TGG + tig0021145:35268-35287 Msa1388550:CDS 50.0%
!! GCGTCTGATTTGGTGAAGAG+CGG + tig0021145:35349-35368 Msa1388550:CDS 50.0%
! CCGGTATTGGATTTGTCTGG+TGG + tig0021145:35433-35452 Msa1388550:CDS 50.0%
!! TTGTCTGGTGGTGATGGAAG+TGG + tig0021145:35445-35464 Msa1388550:CDS 50.0%
AGGATCACAAGGGTTACTCG+CGG - tig0021145:35533-35552 None:intergenic 50.0%
CGATGCCTGAAAAGCCAAAG+TGG + tig0021145:35563-35582 Msa1388550:CDS 50.0%
TGCCTGAAAAGCCAAAGTGG+AGG + tig0021145:35566-35585 Msa1388550:CDS 50.0%
GCCTGAAAAGCCAAAGTGGA+GGG + tig0021145:35567-35586 Msa1388550:CDS 50.0%
GTGGAGGGCTTACAGTAATC+AGG + tig0021145:35582-35601 Msa1388550:CDS 50.0%
GAGGGCTTACAGTAATCAGG+AGG + tig0021145:35585-35604 Msa1388550:CDS 50.0%
GTAATCAGGAGGCTTGGAAG+CGG + tig0021145:35596-35615 Msa1388550:CDS 50.0%
TAATCAGGAGGCTTGGAAGC+GGG + tig0021145:35597-35616 Msa1388550:CDS 50.0%
CTTGACGAGCTTCGTGAAGA+TGG - tig0021145:36929-36948 None:intergenic 50.0%
AGTTCATCTGTGCTAGGCGA+AGG + tig0021145:36947-36966 Msa1388550:CDS 50.0%
TGTGCTAGGCGAAGGAGATT+TGG + tig0021145:36955-36974 Msa1388550:CDS 50.0%
CTAGGCGAAGGAGATTTGGA+CGG + tig0021145:36959-36978 Msa1388550:CDS 50.0%
TAGGCGAAGGAGATTTGGAC+GGG + tig0021145:36960-36979 Msa1388550:CDS 50.0%
TTTCACAGCACGACGACTTG+TGG - tig0021145:36987-37006 None:intergenic 50.0%
CAAGTCGTCGTGCTGTGAAA+CGG + tig0021145:36987-37006 Msa1388550:CDS 50.0%
! ATCAAGCTGAAGTGCCAGAG+TGG + tig0021145:37077-37096 Msa1388550:CDS 50.0%
! AGTGGTTTGGGACCCAAGTA+TGG + tig0021145:37128-37147 Msa1388550:CDS 50.0%
TCCTATAGGAAGGGGAAGAC+AGG + tig0021145:37192-37211 Msa1388550:CDS 50.0%
TCCTGTCTTCCCCTTCCTAT+AGG - tig0021145:37196-37215 None:intergenic 50.0%
AGGGGAAGACAGGATTCATG+CGG + tig0021145:37202-37221 Msa1388550:CDS 50.0%
CGACCTTATCGAAACGCCAA+TGG - tig0021145:37318-37337 None:intergenic 50.0%
CAGCTTTACCCTCCAAAGAG+AGG + tig0021145:37397-37416 Msa1388550:CDS 50.0%
CACCAAGTGTATGCCTGCTA+TGG - tig0021145:37528-37547 None:intergenic 50.0%
TCCAGAGTAGCCACATGGAA+AGG + tig0021145:37615-37634 Msa1388550:three_prime_UTR 50.0%
CCAGAGTAGCCACATGGAAA+GGG + tig0021145:37616-37635 Msa1388550:three_prime_UTR 50.0%
!! CGTTGGTTCGACGCTGAAAC+TGG + tig0021145:34853-34872 Msa1388550:CDS 55.0%
AGTTGTTGTCGGGTCAGGGT+GGG + tig0021145:34981-35000 Msa1388550:CDS 55.0%
GAATGTGGTGGCTGGAGAGA+AGG + tig0021145:35003-35022 Msa1388550:CDS 55.0%
GGTGGCTGGAGAGAAGGTTA+AGG + tig0021145:35009-35028 Msa1388550:CDS 55.0%
GCTGGAGAGAAGGTTAAGGG+TGG + tig0021145:35013-35032 Msa1388550:CDS 55.0%
! TGGAGCTGATGGTGCTAACG+AGG + tig0021145:35033-35052 Msa1388550:CDS 55.0%
CACGGTGGGAATGTGGTAAC+TGG + tig0021145:35097-35116 Msa1388550:CDS 55.0%
TTGGTGAAGAGCGGTGAGTC+TGG + tig0021145:35358-35377 Msa1388550:CDS 55.0%
!!! GCCCTCCACTTTGGCTTTTC+AGG - tig0021145:35571-35590 None:intergenic 55.0%
!! GCGGGTTCTGTTGTTTCGAG+AGG + tig0021145:35615-35634 Msa1388550:CDS 55.0%
TGGGACCCAAGTATGGCCAT+TGG + tig0021145:37135-37154 Msa1388550:CDS 55.0%
AGCTAAGCTGGAGTTAGGCG+TGG + tig0021145:37285-37304 Msa1388550:CDS 55.0%
CCCTTTCCATGTGGCTACTC+TGG - tig0021145:37619-37638 None:intergenic 55.0%
GAGTTGTTGTCGGGTCAGGG+TGG + tig0021145:34980-34999 Msa1388550:CDS 60.0%
GGATTCGGAGCAGTCCACTC+TGG - tig0021145:37094-37113 None:intergenic 60.0%
GTCGGGTCAGGGTGGGAATG+TGG + tig0021145:34988-35007 Msa1388550:CDS 65.0%
GGGTCAGGGTGGGAATGTGG+TGG + tig0021145:34991-35010 Msa1388550:CDS 65.0%
CAGGGTGGGAATGTGGTGGC+TGG + tig0021145:34995-35014 Msa1388550:CDS 65.0%
!! GCATCGGCTCGCATCTGCGT+GGG + tig0021145:37046-37065 Msa1388550:CDS 65.0%
!! CATCGGCTCGCATCTGCGTG+GGG + tig0021145:37047-37066 Msa1388550:CDS 65.0%
TCATGCGGCTGCGCAGTTCC+AGG + tig0021145:37217-37236 Msa1388550:CDS 65.0%
!! GGCATCGGCTCGCATCTGCG+TGG + tig0021145:37045-37064 Msa1388550:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0021145 gene 34722 38041 34722 ID=Msa1388550;Name=Msa1388550
tig0021145 mRNA 34722 38041 34722 ID=Msa1388550-mRNA-1;Parent=Msa1388550;Name=Msa1388550-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|429|583
tig0021145 exon 34722 35684 34722 ID=Msa1388550-mRNA-1:exon:11171;Parent=Msa1388550-mRNA-1
tig0021145 exon 36824 38041 36824 ID=Msa1388550-mRNA-1:exon:11172;Parent=Msa1388550-mRNA-1
tig0021145 CDS 34722 35684 34722 ID=Msa1388550-mRNA-1:cds;Parent=Msa1388550-mRNA-1
tig0021145 CDS 36824 37612 36824 ID=Msa1388550-mRNA-1:cds;Parent=Msa1388550-mRNA-1
tig0021145 three_prime_UTR 37613 38041 37613 ID=Msa1388550-mRNA-1:three_prime_utr;Parent=Msa1388550-mRNA-1
Gene Sequence

>Msa1388550

ATGTTTGATGATGGACAGAAAGTTGATTTGCACAAGCTGTTGATTGTTGTTGACGGGAAAGGTGGGTTTGATGCTGTTTGTGATGGTGAGTTGTGGGATTTGGTTGGGGAAGAATGTGGCTTAGGTGTTAACGTTGGTTCGACGCTGAAACTGGTGTTTAGTAAGTACAAGAGTGTTTTTGAGTCGTGTGTGATGAAAGTTGGTGATGCTAAGGTGTTTGATGAGTCTGGTTTGATGGGGTTTAAGGCTGAGGTGAATGAGTTGTTGTCGGGTCAGGGTGGGAATGTGGTGGCTGGAGAGAAGGTTAAGGGTGGAGCTGATGGTGCTAACGAGGTGAAGAGTGCGAATTTCGAGTCTAGTGGGGAGAAAGAAATTCACGGTGGGAATGTGGTAACTGGAGAGAAGGTTAAGGGTGGAGTTGATGGTGCTAACGAGGTGAAGAGTGTGAATTTTGAGTCTGGTGGGGTGAAAGAAGTTCCCGATGTAGGATTTCTTGATTCCAGAATGATGAATCGAGGGATGGTCAGTGATACCGATGTTGGAAAGCTGAGTGAGGGTAATGGTGATGGAATGGAAGTAGTTGAAGATTTTGATGGAGGGAAAATAACTGTAGGGACAACTGAGGATGCGTCTGATTTGGTGAAGAGCGGTGAGTCTGGATTGCTGAATGTTGGCGAGAATCATGATGGTGATATCGGTGATAATATTGTTCCGGTATTGGATTTGTCTGGTGGTGATGGAAGTGGTTCTAGTCTTAAGAGGAAGAGAGAGGAGGCTCTGTTTGATATGATGAGTTGGGTTAAAAAAACCGCGAGTAACCCTTGTGATCCTGAAATTGTTCCGATGCCTGAAAAGCCAAAGTGGAGGGCTTACAGTAATCAGGAGGCTTGGAAGCGGGTTCTGTTGTTTCGAGAGGCTGCGTTTTACAAGAAAAAGTCGAGCATTGAACAGCAAAATTGGCAGAGTCAGAAGATGCATCCTAGCATGTATGATGATCCCACTCCGACAATCTACAATCTTAGGGAGAGGGGGAAACGTGACAAGAAACTATTTGAAAAACAAAAACCATCTTCACGAAGCTCGTCAAGTTCATCTGTGCTAGGCGAAGGAGATTTGGACGGGACCACAAGTCGTCGTGCTGTGAAACGGTTGCTTGCAAGTAGACGATCAAGTTATAGTAGATGGGCATCGGCTCGCATCTGCGTGGGGCAAAAGTATCAAGCTGAAGTGCCAGAGTGGACTGCTCCGAATCCTGAAAGCGATACTAAGTGGTTTGGGACCCAAGTATGGCCATTGGCTGTATCGAATTCTCAATTTCTTGTCGAAAGGGATCCTATAGGAAGGGGAAGACAGGATTCATGCGGCTGCGCAGTTCCAGGTTCTGTTGAATGTGTCAGATTCCACATTTCTGAGAAAAAAGCTAAAGCTAAGCTGGAGTTAGGCGTGGCTTTTTACCATTGGCGTTTCGATAAGGTCGGTGAAGATGTTCGGAATTCGTGGACAGATGAAGACGAGAAAAGGTTCAGGGAAGTGCTACAGCTTTACCCTCCAAAGAGAGGATATGAACATTTCTGGGATCATATTTTTGAAACATTTCCTAACAAGAGCAGGGAAACTTTAGTTAGCTACTACTTCAATGTCTATCTCCTGCAACGCAAAGCATACCATAGCAGGCATACACTTGGTGACATTCAGAGCGACGATGAAGAATCAGAATCTGATTTGAAGAAGCTTTTTGGACGTCGGGGATAG
Protein sequence

>Msa1388550

MFDDGQKVDLHKLLIVVDGKGGFDAVCDGELWDLVGEECGLGVNVGSTLKLVFSKYKSVFESCVMKVGDAKVFDESGLMGFKAEVNELLSGQGGNVVAGEKVKGGADGANEVKSANFESSGEKEIHGGNVVTGEKVKGGVDGANEVKSVNFESGGVKEVPDVGFLDSRMMNRGMVSDTDVGKLSEGNGDGMEVVEDFDGGKITVGTTEDASDLVKSGESGLLNVGENHDGDIGDNIVPVLDLSGGDGSGSSLKRKREEALFDMMSWVKKTASNPCDPEIVPMPEKPKWRAYSNQEAWKRVLLFREAAFYKKKSSIEQQNWQSQKMHPSMYDDPTPTIYNLRERGKRDKKLFEKQKPSSRSSSSSSVLGEGDLDGTTSRRAVKRLLASRRSSYSRWASARICVGQKYQAEVPEWTAPNPESDTKWFGTQVWPLAVSNSQFLVERDPIGRGRQDSCGCAVPGSVECVRFHISEKKAKAKLELGVAFYHWRFDKVGEDVRNSWTDEDEKRFREVLQLYPPKRGYEHFWDHIFETFPNKSRETLVSYYFNVYLLQRKAYHSRHTLGDIQSDDEESESDLKKLFGRRG*